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CD63 exosome

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https://www.readbyqxmd.com/read/27882487/delivery-of-small-interfering-rna-to-inhibit-vascular-endothelial-growth-factor-in-zebrafish-using-natural-brain-endothelia-cell-secreted-exosome-nanovesicles-for-the-treatment-of-brain-cancer
#1
Tianzhi Yang, Brittany Fogarty, Bret LaForge, Salma Aziz, Thuy Pham, Leanne Lai, Shuhua Bai
Although small interfering RNA (siRNA) holds great therapeutic promise, its delivery to the disease site remains a paramount obstacle. In this study, we tested whether brain endothelial cell-derived exosomes could deliver siRNA across the blood-brain barrier (BBB) in zebrafish. Natural exosomes were isolated from brain endothelial bEND.3 cell culture media and vascular endothelial growth factor (VEGF) siRNA was loaded in exosomes with the assistance of a transfection reagent. While fluorescence-activated cell flow cytometry and immunocytochemistry staining studies indicated that wild-type exosomes significantly increased the uptake of fluorescence-labeled siRNA in the autologous brain endothelial cells, decreased fluorescence intensity was observed in the cells treated with the tetraspanin CD63 antibody-blocked exosome-delivered formulation (p < 0...
November 23, 2016: AAPS Journal
https://www.readbyqxmd.com/read/27881345/-extraction-and-identification-of-semen-derived-exosomes-using-peg6000
#2
Cheng Yang, Wen-Bin Guo, Wan-Song Zhang, Jun Bian, Jian-Kun Yang, Tao Qi, Chun-Yan Wang, Cun-Dong Liu
OBJECTIVE: To extract and identify semen-derived exosome using PEG6000. METHODS: Exosomes were extracted from semen specimens from 6 healthy volunteers with step-by-step centrifugations and ultracentrifugation prior to 8% PEG6000 enrichment. The extracted exosomes were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting. RESULTS: The pellets obtained were round or elliptic membrane vesicles 30 to 150 nm in diameter with intact double membranes and contained low electron density material...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27872619/ebola-vp40-in-exosomes-can-cause-immune-cell-dysfunction
#3
Michelle L Pleet, Allison Mathiesen, Catherine DeMarino, Yao A Akpamagbo, Robert A Barclay, Angela Schwab, Sergey Iordanskiy, Gavin C Sampey, Benjamin Lepene, Sergei Nekhai, M J Aman, Fatah Kashanchi
Ebola virus (EBOV) is an enveloped, ssRNA virus from the family Filoviridae capable of causing severe hemorrhagic fever with up to 80-90% mortality rates. The most recent outbreak of EBOV in West Africa starting in 2014 resulted in over 11,300 deaths; however, long-lasting persistence and recurrence in survivors has been documented, potentially leading to further transmission of the virus. We have previously shown that exosomes from cells infected with HIV-1, HTLV-1 and Rift Valley Fever virus are able to transfer viral proteins and non-coding RNAs to naïve recipient cells, resulting in an altered cellular activity...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27856179/exosomal-proteins-as-prognostic-biomarkers-in-non-small-cell-lung-cancer
#4
B Sandfeld-Paulsen, N Aggerholm-Pedersen, R Bæk, K R Jakobsen, P Meldgaard, B H Folkersen, T R Rasmussen, K Varming, M M Jørgensen, B S Sorensen
BACKGROUND: Use of exosomes as biomarkers in non-small cell lung cancer (NSCLC) is an intriguing approach in the liquid-biopsy era. Exosomes are nano-sized vesicles with membrane-bound proteins that reflect their originating cell. Prognostic biomarkers are needed to improve patient selection for optimal treatment. We here evaluate exosomes by protein phenotyping as a prognostic biomarker in NSCLC. METHODS: Exosomes from plasma of 276 NSCLC patients were phenotyped using the Extracellular Vesicle Array; 49 antibodies captured the proteins on the exosomes, and a cocktail of biotin-conjugated antibodies binding the general exosome markers CD9, CD81 and CD63 was used to visualise the captured exosomes...
October 21, 2016: Molecular Oncology
https://www.readbyqxmd.com/read/27802194/screening-of-exosomal-micrornas-from-colorectal-cancer-cells
#5
Cillian Clancy, Sonja Khan, Claire L Glynn, Emma Holian, Peter Dockery, Pierce Lalor, James A L Brown, Myles R Joyce, Michael J Kerin, Roisin M Dwyer
BACKGROUND: Cells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown. OBJECTIVE: To identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRNAs. METHODS: Exosomes were isolated from HCT-116 and HT-29 cell lines...
September 30, 2016: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/27795705/cd63-and-glut-1-overexpression-could-predict-a-poor-clinical-outcome-in-gist-a-study-of-54-cases-with-follow-up
#6
Piotr Lewitowicz, Jarosław Matykiewicz, Dorota Koziel, Magdalena Chrapek, Agata Horecka-Lewitowicz, Stanislaw Gluszek
Background and Goals. In light of current knowledge, it seems that alternations underlying GISTs are well explained, although all that is enhanced by various aspects on a daily basis. More recently, attention has been pointed towards exosomes as important particles able to modify healthy and also diseased tissues including cancer. The goal of the present study was an analysis of CD9, CD63, and GLUT-1 as a marker of hypoxia status within 54 cases of GIST and evaluation of their predictive value. Methods. 54 cases of patients suffering from GIST were enrolled into the study, predominantly in the gastric location...
2016: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/27779585/exosomes-not-protein-or-lipids-in-mesenteric-lymph-activate-inflammation-unlocking-the-mystery-of-post-shock-multiple-organ-failure
#7
Mitsuaki Kojima, Joao Antonio Gimenes-Junior, Simone Langness, Koji Morishita, Ophelie Lavoie-Gagne, Brian Eliceiri, Todd W Costantini, Raul Coimbra
BACKGROUND: Previous studies have shown that mesenteric lymph (ML) has a crucial role in driving the systemic inflammatory response after trauma/hemorrhagic shock (T/HS). The specific mediators in the ML that contribute to its biological activity remain unclear despite decades of study. Exosomes are extracellular vesicles that are shed into body fluids such as serum and urine that can mediate intercellular communication. We hypothesized that exosomes are present in the ML after trauma/shock and are responsible for the biological activity of ML...
October 25, 2016: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/27765945/different-populations-of-wnt-containing-vesicles-are-individually-released-from-polarized-epithelial-cells
#8
Qiuhong Chen, Ritsuko Takada, Chiyo Noda, Satoru Kobayashi, Shinji Takada
Accumulating evidence suggests that exosomes are heterogeneous in molecular composition and physical properties. Here we examined whether epithelial cells secrete a heterogeneous population of exosomes, and if that is the case, whether epithelial cell polarity affects release of different populations of exosomes, especially that of those carrying Wnt. Sucrose-density ultracentrifugation and molecular marker analysis revealed that different populations of exosomes or exosome-like vesicles were released from MDCK cells depending on the cell polarity...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27725852/microrna-155-in-exosomes-secreted-from-helicobacter-pylori-infection-macrophages-immunomodulates-inflammatory-response
#9
Jianjun Wang, Zhiyong Deng, Zeyou Wang, Jianhong Wu, Tao Gu, Yibiao Jiang, Guangxin Li
Exosomes containing microRNA-155 act as molecule carriers during immune cell-cell communication and play an important role in the inflammatory response of H. pylori infection macrophages. Previous reports have found that miR-155 was over-expressed in H. pylori infection macrophages, but the significance of which is still unknown. In this study, we analyzed the impact of miR-155 loaded in exosomes derived from macrophages to the inflammatory response of H. pylori infection macrophages and possible mechanisms...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27722146/quantitative-analysis-of-exosomes-from-murine-lung-cancer-cells-by-flow-cytometry
#10
Kyung-Taek Rim, Soo-Jin Kim
In vivo studies regarding biochemical, molecular biological, and histopathological changes in cancer tissues have been widely performed by the administration of carcinogens in rodents. In these established methods, dissection of the animal following sacrifice must be carried out. Exosomes are cell-derived vesicles that are present in all body fluids and these vesicles have specific roles within cells. Thus, much attention is given to the clinical application of exosomes that can possibly be used for prediction and therapy and as biomarkers related to cancer...
September 2016: Journal of Cancer Prevention
https://www.readbyqxmd.com/read/27716356/exosomes-derived-from-hcc-cells-induce-sorafenib-resistance-in-hepatocellular-carcinoma-both-in-vivo-and-in-vitro
#11
Zhen Qu, Junhua Wu, Junyi Wu, Dongjun Luo, Chunping Jiang, Yitao Ding
BACKGROUND: Exosomes are carriers of intercellular information and regulate the tumor microenvironment. They play an important role in drug resistance by transporting RNA molecules and proteins. However, their effects on sorafenib resistance in hepatocellular carcinoma (HCC) are not completely understood. METHODS: Exosomes were isolated from two invasive hepatoma cell lines (MHCC-97 L and MHCC-97H), and their roles in regulating sorafenib resistance in liver cancer cells as well as the underlying molecular mechanisms were determined...
September 30, 2016: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/27707921/the-intra-cellular-cholesterol-transport-inhibitor-u18666a-inhibits-the-exosome-dependent-release-of-mature-hepatitis-c-virus
#12
Fabian Elgner, Huimei Ren, Regina Medvedev, Daniela Ploen, Kiyoshi Himmelsbach, Klaus Boller, Eberhard Hildt
BACKGROUND: HCV particles are described as lipoviroparticles which are released similar to VLDLs. However, the release mechanism is still poorly understood; the canonical ERGIC pathway as well as the endosome-dependent release has been proposed. Recently, also the role of exosomes in the transmission of HCV has been reported. Only a minor fraction of the de novo synthesized lipoviroparticles are finally released by the infected cell. METHODS: To investigate the relevance of MVBs for viral morphogenesis and release, the MVB inhibitor U18666A was used...
October 5, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27697217/placental-exosomes-and-pre-eclampsia-maternal-circulating-levels-in-normal-pregnancies-and-early-and-late-onset-pre-eclamptic-pregnancies
#13
Preenan Pillay, Niren Maharaj, Jagidesa Moodley, Irene Mackraj
INTRODUCTION AND AIM: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biological cells under normal and pathological conditions. Although there have been reports of circulating exosomes in normal pregnancy, the relevance of placental-derived exosomes in normal and abnormal pregnancies still needs to be elucidated. The aim of this study was to quantify total and placental-derived exosomes in maternal plasma from normal (N), early onset- and late onset-preeclampsia (PE)...
October 2016: Placenta
https://www.readbyqxmd.com/read/27644331/dual-wavelength-surface-plasmon-resonance-for-determining-the-size-and-concentration-of-sub-populations-of-extracellular-vesicles
#14
Deborah L M Rupert, Ganesh V Shelke, Gustav Emilsson, Virginia Claudio, Stephan Block, Cecilia Lässer, Andreas Dahlin, Jan O Lötvall, Marta Bally, Vladimir P Zhdanov, Fredrik Höök
Accurate concentration determination of subpopulations of extracellular vesicles (EVs), such as exosomes, is of importance both in the context of understanding their fundamental biological role and of potentially using them as disease biomarkers. In principle, this can be achieved by measuring the rate of diffusion-limited mass uptake to a sensor surface modified with a receptor designed to only bind the subpopulation of interest. However, a significant error is introduced if the targeted EV subpopulation has a size, and thus hydrodynamic diffusion coefficient, that differs from the mean size and diffusion coefficient of the whole EV population and/or if the EVs become deformed upon binding to the surface...
October 4, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27599779/quantification-of-plasma-exosome-is-a-potential-prognostic-marker-for-esophageal-squamous-cell-carcinoma
#15
Yasunori Matsumoto, Masayuki Kano, Yasunori Akutsu, Naoyuki Hanari, Isamu Hoshino, Kentaro Murakami, Akihiro Usui, Hiroshi Suito, Masahiko Takahashi, Ryota Otsuka, Hu Xin, Aki Komatsu, Keiko Iida, Hisahiro Matsubara
Exosomes play important roles in cancer progression. Although its contents (e.g., proteins and microRNAs) have been focused on in cancer research, particularly as potential diagnostic markers, the exosome behavior and methods for exosome quantification remain unclear. In the present study, we analyzed the tumor-derived exosome behavior and assessed the quantification of exosomes in patient plasma as a biomarker for esophageal squamous cell carcinoma (ESCC). A CD63-GFP expressing human ESCC cell line (TE2-CD63-GFP) was made by transfection, and mouse subcutaneous tumor models were established...
September 5, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27592404/hepatoprotective-effect-of-exosomes-from-human-induced-pluripotent-stem-cell-derived-mesenchymal-stromal-cells-against-hepatic-ischemia-reperfusion-injury-in-rats
#16
Kate Nong, Weiwei Wang, Xin Niu, Bin Hu, Chenchao Ma, Yueqing Bai, Bo Wu, Yang Wang, Kaixing Ai
BACKGROUND: This study aimed to evaluate the effect of exosomes produced by human-induced pluripotent stem cell-derived mesenchymal stromal cells (hiPSC-MSCs-Exo) on hepatic ischemia-reperfusion (I/R) injury. METHODS: Exosomes were isolated and concentrated from conditioned medium using ultracentrifugation and ultrafiltration. hiPSC-MSCs-Exo were injected systemically via the inferior vena cava in a rat model of 70% warm hepatic I/R injury, and the therapeutic effect was evaluated...
August 31, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27579288/microvesicles-preparation-from-mesenchymal-stem-cells
#17
Fariba Rad, Ali Akbar Pourfathollah, Fatemeh Yari, Saeed Mohammadi, Maryam Kheirandish
BACKGROUND: Extracellular vesicles are particles ranged from 30 nm to 5μm and subcategorized into three groups; exosomes, microvesicles and apoptotic bodies, each of which have different biological impact. Lack of a standard method for the detection and isolation of MVs has led to a challenging issue that is a worth considering. In this study, we isolated MVs from the conditioned medium of UC-MSCs by four different schemes of ultracentrifugation. METHODS: We examined the efficacy of differential centrifugation ranging from 10,000×g to 60,000×g on UCMSCs- derived microvesicles yield and purity...
2016: Medical Journal of the Islamic Republic of Iran
https://www.readbyqxmd.com/read/27568281/the-impact-of-various-preanalytical-treatments-on-the-phenotype-of-small-extracellular-vesicles-in-blood-analyzed-by-protein-microarray
#18
Rikke Bæk, Evo K L Søndergaard, Kim Varming, Malene M Jørgensen
The research field of extracellular vesicles (EVs) is increasing immensely and the potential uses of EVs seem endless. They are found in large numbers in various body fluids, and blood samples may well serve as liquid biopsies. However, these small membrane-derived entities of cellular origin are not straightforward to work with in regard to isolation and characterization. A broad range of relevant preanalytical issues was tested, with a focus on the phenotypic impact of smaller EVs. The influences of the i) blood collection tube used, ii) incubation time before the initial centrifugation, iii) transportation/physical stress, iv) storage temperature and time (short term and long term), v) choice of centrifugation protocol, vi) freeze-thaw cycles, and vii) exosome isolation procedure (ExoQuick™) were examined...
November 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/27559612/y-box-protein-1-is-required-to-sort-micrornas-into-exosomes-in-cells-and-in-a-cell-free-reaction
#19
Matthew J Shurtleff, Morayma M Temoche-Diaz, Kate V Karfilis, Sayaka Ri, Randy Schekman
Exosomes are small vesicles that are secreted from metazoan cells and may convey selected membrane proteins and small RNAs to target cells for the control of cell migration, development and metastasis. To study the mechanisms of RNA packaging into exosomes, we devised a purification scheme based on the membrane marker CD63 to isolate a single exosome species secreted from HEK293T cells. Using immunoisolated CD63-containing exosomes we identified a set of miRNAs that are highly enriched with respect to their cellular levels...
August 25, 2016: ELife
https://www.readbyqxmd.com/read/27542209/boar-seminal-plasma-exosomes-maintain-sperm-function-by-infiltrating-into-the-sperm-membrane
#20
Jian Du, Jian Shen, Yuanxian Wang, Chuanying Pan, Weijun Pang, Hua Diao, Wuzi Dong
Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect of boar seminal plasma exosomes on sperm function during long-term liquid storage. Boar seminal plasma exosomes had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such as AWN, CD9 and CD63 by western blot analysis. The effect on sperm parameters of adding different ratio of boar seminal plasma exosomes to boar sperm preparations was analyzed...
August 16, 2016: Oncotarget
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