Hejie Wang, Minmin Dou, Jun Li, Peng Cao, Junling Li, Tianyan Guo, Dipeng Zhao, Ajab Khan, Yingliang Li, Baojun Li, Jian Qin, Rong Du
The purpose of this study was to establish a system for the isolation, culture, and differentiation of sheep myoblasts, and to explore the expression patterns as well as mutual relationships of muscle-specific genes. Sheep fetal myoblasts (SFMs) were isolated by two-step enzymatic digestion, purified by differential adhesion and identified using immunofluorescence techniques. Two percent horse serum was used to induce differentiation in SFMs. Real-time quantitative and Western blot analyses were respectively used to detect the mRNA and protein expressions of muscle-specific genes including MyoD, MyoG, Myf5, Myf6, PAX3, PAX7, myomaker, desmin, MYH1, MYH2, MYH4, MYH7, and MSTN during the differentiation of SFMs...
September 30, 2022: In Vitro Cellular & Developmental Biology. Animal