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https://www.readbyqxmd.com/read/28522710/draft-genome-sequences-of-the-xylose-fermenting-yeast-scheffersomyces-shehatae-nbrc-1983-t-and-a-thermotolerant-isolate-of-s-%C3%A2-shehatae-aty839-jcm-18690
#1
Natsumi Okada, Ayumi Tanimura, Hideki Hirakawa, Masako Takashima, Jun Ogawa, Jun Shima
Draft genome sequences of the type strain (NBRC 1983) and a thermotolerant isolate (ATY839) of the xylose-fermenting yeast Scheffersomyces shehatae were determined. The genome sizes and presumed open reading frames were highly similar between strains NBRC 1983(T) and ATY839.
May 18, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28474089/erythrobacter-alti-sp-nov-a-marine-alphaproteobacterium-isolated-from-seawater
#2
Jaewoo Yoon
A polyphasic taxonomic study was performed on strain KMU-34(T), which was isolated from seawater in the Republic of Korea. The bacterial cells were Gram-stain-negative, strictly aerobic, chemoheterotrophic, yellow-pigmented and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain was affiliated with the family Erythrobacteraceae of the class Alphaproteobacteria and that it showed highest sequence similarity (98.6%) to Erythrobacter atlanticus s21-N3(T)...
May 4, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28471330/characterization-and-gene-cloning-of-l-xylulose-reductase-involved-in-l-arabinose-catabolism-from-the-pentose-fermenting-fungus-rhizomucor-pusillus
#3
Shino Yamasaki-Yashiki, Hidenobu Komeda, Kazuhiro Hoshino, Yasuhisa Asano
l-Xylulose reductase (LXR) catalyzes the reduction of l-xylulose to xylitol in the fungal l-arabinose catabolic pathway. LXR (RpLXR) was purified from the pentose-fermenting zygomycetous fungus Rhizomucor pusillus NBRC 4578. The native RpLXR is a homotetramer composed of 29 kDa subunits and preferred NADPH as a coenzyme. The Km values were 8.71 mM for l-xylulose and 3.89 mM for dihydroxyacetone. The lxr3 (Rplxr3) gene encoding RpLXR consists of 792 bp and encodes a putative 263 amino acid protein (Mr = 28,341)...
May 4, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28466301/comparative-metabolomic-studies-of-alkanivorax-xenomutans-showing-differential-power-output-in-a-three-chambered-microbial-fuel-cell
#4
Ganesh Mahidhara, Sasikala Ch, Venkata Ramana Ch
Metabolomic study of electrogenic bacteria is a necessity to understand the extent of complex organic matter degradation and to invent new co-culture techniques to achieve complete degradation. In this study, we have subjected Alkanivorax xenomutans (KCTC 23751(T); NBRC 108843(T)), a bacterium capable for biodegradation of complex hydrocarbons, to oxic and anoxic conditions in a three chambered microbial fuel cell. In an attempt to understand the molecular mechanisms during the electrogenic processes of A. xenomutans, intra cellular (endo metabolome or the fingerprint) and exo metabolome (extracellular metabolome or the foot print) were analyzed under oxic and anoxic conditions, using FTIR and GC-MS...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28466299/streptomyces-sp-at37-isolated-from-a-saharan-soil-produces-a-furanone-derivative-active-against-multidrug-resistant-staphylococcus-aureus
#5
El Hadj Driche, Nasserdine Sabaou, Christian Bijani, Abdelghani Zitouni, Frédéric Pont, Florence Mathieu, Boubekeur Badji
A novel actinobacterium strain, named AT37, showed a strong activity against some multidrug-resistant Staphylococcus aureus, including methicillin-resistant S. aureus MRSA ATCC 43300, other clinical isolates of MRSA and vancomycin resistant S. aureus VRSA S1. The strain AT37 was isolated from a Saharan soil by a dilution agar plating method using chitin-vitamin agar medium supplemented with rifampicin. The morphological and chemical studies indicated that this strain belonged to the genus Streptomyces. Its 16S rRNA gene sequence was determined and a database search indicated that it was closely associated with the type strain of Streptomyces novaecaesareae NBRC 13368(T) with 99...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28434178/massilia-chloroacetimidivorans-sp-nov-a-chloroacetamide-herbicide-degrading-bacterium-isolated-from-soil
#6
Hyosun Lee, Dong-Uk Kim, Sooyeon Park, Jung-Hoon Yoon, Jong-Ok Ka
A Gram-stain negative, rod-shaped, aerobic bacterial strain, designated TA-C7e(T), was isolated from an agricultural soil obtained from Taean region, Korea. Phylogenetically, the strain was grouped with the genus Massilia and shared 95.33-97.97% 16S rRNA sequence similarity with the type strains of other species of the genus Massilia. The DNA-DNA hybridisation between TA-C7e(T) and Massilia haematophila KACC 13771(T), Massilia suwonesis KACC 12635(T), and Massilia yuzhufengensis KACC 16569(T) revealed a low level of relatedness (<40%)...
April 22, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28428295/multiple-genome-sequences-of-heteropolysaccharide-forming-acetic-acid-bacteria
#7
Julia U Brandt, Frank Jakob, Andreas J Geissler, Jürgen Behr, Rudi F Vogel
We report here the complete genome sequences of the acetic acid bacteria (AAB) Acetobacter aceti TMW 2.1153, A. persici TMW 2.1084, and Neoasaia chiangmaiensis NBRC 101099, which secrete biotechnologically relevant heteropolysaccharides (HePSs) into their environments. Upon genome sequencing of these AAB strains, the corresponding HePS biosynthesis pathways were identified.
April 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28401363/characterization-of-marinovum-faecis-sp-nov-an-alphaproteobacterium-isolated-from-marine-sediment
#8
Jaewoo Yoon, So Young Kim, Songhee Park, Hyukjae Choi
A Gram-negative, strictly aerobic, chemoheterotrophic, beige-pigmented, ovoid bacterium, designated YP194(T), was isolated from marine sediment in the Republic of Korea. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain belongs to the family Rhodobacteraceae of the class Alphaproteobacteria, with high sequence similarity (98.4%) to Marinovum algicola FF3(T). The DNA-DNA relatedness values between strains YP194(T) and M. algicola FF3(T) were 34.1 ± 2.7%. The DNA G+C content of strain YP194(T) was 63...
April 11, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28400025/influence-of-levan-producing-acetic-acid-bacteria-on-buckwheat-sourdough-breads
#9
Tharalinee Ua-Arak, Frank Jakob, Rudi F Vogel
Buckwheat sourdoughs supplemented with molasses as natural sucrose source were fermented with levan-producing Gluconobacter (G.) albidus TMW 2.1191 and Kozakia (K.) baliensis NBRC 16680. Cell growth, concomitant levan and low-molecular-weight metabolite production were monitored. Sourdough breads were prepared with different sourdoughs from both strains (24, 30 and 48 h fermentation, respectively) and analyzed with respect to bread volume, crumb hardness and sensory characteristics. During fermentation, levan, acetic and gluconic acids were increasingly produced, while spontaneously co-growing lactic acid bacteria additionally formed acetic and lactic acids...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28378221/bioresolution-of-racemic-phenyl-glycidyl-ether-by-a-putative-recombinant-epoxide-hydrolase-from-streptomyces-griseus-nbrc-13350
#10
Priya Saini, Naveen Kumar, Shadil Ibrahim Wani, Shilpi Sharma, Swapandeep Singh Chimni, Dipti Sareen
In order to produce enantiomerically pure epoxides for the synthesis of value-added chemicals, a novel putative epoxide hydrolase (EH) sgeh was cloned and overexpressed in pET28a/Escherichia coli BL21(DE3). The 1047 bp sgeh gene was mined from Streptomyces griseus NBRC 13350 genome sequence. The recombinant hexahistidyl-tagged SGEH was purified (16.6-fold) by immobilized metal-affinity chromatography, with 90% yield as a homodimer of 100 kDa. The recombinant E. coli whole cells overexpressing SGEH could kinetically resolve racemic phenyl glycidyl ether (PGE) into (R)-PGE with 98% ee, 40% yield, and enantiomeric ratio (E) of 20...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28360164/draft-genome-sequence-of-caenibacillus-caldisaponilyticus-b157-t-a-thermophilic-and-phospholipase-producing-bacterium-isolated-from-acidulocompost
#11
Yoshiyuki Tsujimoto, Ryo Saito, Takehiko Sahara, Nobutada Kimura, Naoki Tsuruoka, Yasushi Shigeri, Kunihiko Watanabe
Caenibacillus caldisaponilyticus B157(T) (= NBRC 111400(T) = DSM 101100(T)), in the family Sporolactobacillaceae, was isolated from acidulocompost as a thermophilic and phospholipid-degrading bacterium. Here, we report the 3.36-Mb draft genome sequence, with a G+C content of 51.8%, to provide the genetic information coding for phospholipases.
March 30, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28342817/complete-genome-sequence-of-streptomyces-griseochromogenes-atcc-14511-t-a-producer-of-nucleoside-compounds-and-diverse-secondary-metabolites
#12
Lijuan Wu, Guoqing Chen, Guozhong Feng
ATCC 14511(T) (=DSM 40499, =NBRC 13413) is a type strain of Streptomyces griseochromogenes. It is known as a producer of nucleoside antibiotic, blasticidin S. In this report we present the complete genome sequence of S. griseochromogenes ATCC 14511(T), which consists of 10,764,674bp with a linear chromosome, 9822 protein-coding genes, 6 rRNA operons, 74 tRNA and 3 sRNA. The genomic analysis revealed that 52 putative gene clusters are involved in the biosynthesis of secondary metabolites, including four gene clusters of nucleoside antibiotics...
March 22, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28280009/complete-genome-sequence-of-spiroplasma-sp-nbrc-100390
#13
Mindia Haryono, Wen-Sui Lo, Gail E Gasparich, Chih-Horng Kuo
Spiroplasma sp. NBRC 100390 was initially described as a duplicate of S. atrichopogonis GNAT3597(T) (=ATCC BAA-520(T)) but later found to be different in the 16S rDNA sequences. Here, we report the complete genome sequence of this bacterium to establish its identity and to facilitate future investigation.
March 9, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28260703/streptomyces-cameroonensis-sp-nov-a-geldanamycin-producer-that-promotes-theobroma-cacao-growth
#14
Thaddée Boudjeko, Romaric Armel Mouafo Tchinda, Mina Zitouni, Joëlle Aimée Vera Tchatchou Nana, Sylvain Lerat, Carole Beaulieu
The taxonomy of an actinobacterial strain, designated JJY4(T), was established using a polyphasic approach. JJY4(T) was isolated from the rhizosphere of Chromolaena odorata in Yaoundé (Cameroon) during a project for the selection of biological control agents. Strain JJY4(T) exhibited antimicrobial activities against bacteria, fungi, and oomycetes. Strain JJY4(T) also exhibited the traits of plant growth-promoting rhizobacteria such as the solubilization of inorganic phosphate, production of siderophores and indole-3-acetic acid, and 1-aminocyclopropane-1-carboxylate deaminase activity...
March 31, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28251548/jindonia-aestuariivivens-gen-nov-sp-nov-isolated-from-a-tidal-flat-on-the-south-western-sea-in-republic-of-korea
#15
Sooyeon Park, Sun Young Yoon, Min-Ji Ha, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-flagellated, and coccoid, ovoid or rod-shaped bacterium, designated JDTF-65(T), was isolated from a tidal flat on the south-western sea in Republic of Korea. Strain JDTF-65(T) grew optimally at 25°C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Strain JDTF-65(T) exhibited 16S rRNA gene sequence similarities of 97.1-97.6% to the type strains of 'Aliisedimentitalea scapharcae', Phaeobacter gallaeciensis, Phaeobacter inhibens, Leisingera aquimarina, Tropicibacter litoreus, Sulfitobacter pseudonitzschiae, and Pseudoseohaeicola caenipelagi...
March 1, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28231272/vibrio-japonicus-sp-nov-a-novel-member-of-the-nereis-clade-in-the-genus-vibrio-isolated-from-the-coast-of-japan
#16
Hiroyasu Doi, Ikuko Osawa, Hayamitsu Adachi, Manabu Kawada
A novel Vibrio strain, JCM 31412T, was isolated from seawater collected from the Inland Sea (Setonaikai), Japan, and characterized as a Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, ovoid-shaped bacterium with one polar flagellum. Based on 16S rDNA gene identity, strain JCM 31412T showed a close relationship with type strains of Vibrio brasiliensis (LMG 20546T, 98.2% identity), V. harveyi (NBRC 15634T, 98.2%), V. caribbeanicus (ATCC BAA-2122T, 97.8%) and V. proteolyticus (NBRC 13287T, 97...
2017: PloS One
https://www.readbyqxmd.com/read/28209826/complete-genome-sequence-of-the-polychlorinated-biphenyl-degrading-bacterium-pseudomonas-putida-kf715-nbrc-110667-isolated-from-biphenyl-contaminated-soil
#17
Hikaru Suenaga, Atsushi Yamazoe, Akira Hosoyama, Nobutada Kimura, Jun Hirose, Takahito Watanabe, Hidehiko Fujihara, Taiki Futagami, Masatoshi Goto, Kensuke Furukawa
Pseudomonas putida KF715 (NBRC 110667) utilizes biphenyl as a sole source of carbon and degrades polychlorinated biphenyls (PCBs). Here, we report a complete genome sequence of the KF715 strain, which comprises a circular chromosome and four plasmids. Biphenyl catabolic genes were located on the largest plasmid, pKF715A.
February 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28209823/draft-genome-sequencing-of-the-highly-halotolerant-and-allopolyploid-yeast-zygosaccharomyces-rouxii-nbrc-1876
#18
Atsushi Sato, Kenichiro Matsushima, Kenshiro Oshima, Masahira Hattori, Yasuji Koyama
The highly halotolerant and allopolyploid yeast Zygosaccharomyces rouxii is industrially used for the food production in high concentrations of salt, such as brewing soy sauce and miso paste. Here, we report the draft genome sequence of Z. rouxii NBRC 1876 isolated from miso paste.
February 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28174421/streptomyces-euryhalinus-sp-nov-a-new-actinomycete-isolated-from-a-mangrove-forest
#19
Kaushik Biswas, Jayanta D Choudhury, Riddhi Mahansaria, Malay Saha, Joydeep Mukherjee
A Gram-positive, aerobic, non-motile actinomycete (strain MS 3/20(T)) was isolated from the sediment of the Sundarbans mangrove forest in India. On International Streptomyces Project (ISP) medium 2, the isolate produced yellowish brown to red aerial hyphae that carried spiny-surfaced spores in a retinaculum-apertum arrangement. Whole-cell hydrolysate of the strain contained LL-diaminopimelic acid and galactose. Predominant menaquinones were MK-9(H8) and MK-9(H6). Diagnostic polar lipids were glycolipid, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, unidentified phospholipid and unidentified amino lipid...
February 8, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28155814/nonomuraea-rhodomycinica-sp-nov-isolated-from-peat-swamp-forest-soil-in-thailand
#20
Paranee Sripreechasak, Wongsakorn Phongsopitanun, Khomsan Supong, Pattama Pittayakhajonwut, Takuji Kudo, Moriya Ohkuma, Somboon Tanasupawat
The taxonomic position of actinomycete, strain NR4-ASC07T isolated from soil sample collected from Sirindhorn peat swamp forest, Narathiwat Province, Thailand, was clarified using a polyphasic approach. On the basis of morphological and chemotaxonomic characteristics, it was classified in the members of the genus Nonomuraea. It produced tightly closed spiral spore chains on aerial mycelium as well as forming the pseudosporangium. Whole-cell hydrolysate contained meso-diaminopimelic acid, glucose, ribose, madurose and mannose...
February 2, 2017: International Journal of Systematic and Evolutionary Microbiology
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