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Renwen Zhang, Xiaoxue Han, Zhanfeng Xia, Xiaoxia Luo, Chuanxing Wan, Lili Zhang
A novel actinomycete strain, designated TRM 49605(T), was isolated from a desert soil sample from Lop Nur, Xinjiang, north-west China, and characterised using a polyphasic taxonomic approach. The strain exhibited antifungal activity against the following strains: Saccharomyces cerevisiae, Curvularia lunata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Penicillium citrinum, Candida albicans and Candida tropicalis; Antibacterial activity against Bacillus subtilis, Staphylococcus epidermidis and Micrococcus luteus; and no antibacterial activity against Escherichia coli...
October 17, 2016: Antonie Van Leeuwenhoek
Hisayuki Komaki, Akira Hosoyama, Shuhei Yabe, Akira Yokota, Yoshihito Uchino, Hideaki Takano
Here, we report the draft genome sequence of Thermogemmatispora onikobensis NBRC 111776(T), an aerial mycelium- and spore-forming thermophilic bacterium belonging to the class Ktedonobacteria The genome contains five biosynthetic gene clusters coding for secondary metabolites, such as terpene, thiopeptide, lantipeptide, nonribosomal peptide, and lassopeptide, suggesting the potential to produce secondary metabolites.
October 13, 2016: Genome Announcements
Petra Wrent, Eva-María Rivas, José M Peinado, María-Isabel de Silóniz
Based on IGS-PCR RFLP polymorphism, we previously detected two Z. rouxii strains (CECT 11923 and CECT 10425) that clustered with hybrid strains (NCYC 1682, NCYC 3060 and NCYC 3061). Given the recently recognized important industrial role of hybrids, their detection is very useful. Based on the IGS1 rDNA region alignment of hybrid strains and the Z. rouxii CECT 11923 and CECT 10425, in this work, we developed a pair of Zygosaccharomyces hybrid-specific primers, HibZF/HibZR. Positive amplicons were only obtained in the Zygosaccharomyces spp...
September 25, 2016: International Journal of Food Microbiology
Julia U Brandt, Frank Jakob, Jürgen Behr, Andreas J Geissler, Rudi F Vogel
BACKGROUND: Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan. Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose. Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources...
October 4, 2016: Microbial Cell Factories
Hua-Lei Wu, Jian-Dong Zhang, Chao-Feng Zhang, Xiao-Jun Fan, Hong-Hong Chang, Wen-Long Wei
Four uncharacterized ω-transaminases (ωTAs) from Pseudomonas putida NBRC 14164 have been identified and cloned from the pool of fully sequenced genomes. The genes were functionally expressed in Escherichia coli BL21, and the enzymes were purified and characterized. Four TAs showed highly (S)-selective ωTA activity and converted (S)-α-methylbenzylamine and pyruvate to acetophenone and L-Ala. The maximum activity of cloned enzymes was in the pH range of 8.0-8.5 (Pp36420), 8.5-9.5 (Pp21050), 9.0-9.5 (PpspuC), and 9...
October 6, 2016: Applied Biochemistry and Biotechnology
Sooyeon Park, Min-Ji Ha, Sun Young Yoon, Yong-Taek Jung, Jung-Hoon Yoon
A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-19T, was isolated from a tidal flat in oido, an island of South Korea, and subjected to polyphasic taxonomic study. Strain OITF-19T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain OITF-19T belonged to the genus Algoriphagus, clustering with the type strain of A. namhaensis showing 96...
September 30, 2016: International Journal of Systematic and Evolutionary Microbiology
Jong-Hwa Kim, Jung-Hoon Yoon, Wonyong Kim
Gram-stain-negative, non-motile, non-spore-forming, ovoid rod bacterium, designated CAU1172T, was isolated from lagoon sediments along the east coast in Republic of Korea. Strain CAU1172T formed yellow-pigment on marine agar. Growth occurred at 20-37C (optimum, 30C), at pH 6.5-10 (optimum, 7.5), and in the presence of 0-4 % NaCl (optimum, 1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain CAU1172T formed a separate lineage within the genus Altererythrobacter, and was most closely related to Altererythrobacter gangjinensis KJ7T (96...
September 29, 2016: International Journal of Systematic and Evolutionary Microbiology
Hideyuki Kouzui, Koki Tokikawa, Masataka Satomi, Takumi Negoro, Katsuya Shimabukuro, Katsuhiko Fujii
Cellulolytic and agarolytic bacterial strain 12-2T was isolated from a piece of cotton rope fragment washed ashore on a beach and was studied phenotypically, genotypically and phylogenetically. Analyses of 16S rRNA gene and gyrB sequences and DNA base composition suggested that the strain is a member of Gilvimarinus. However, similarities of 16S rRNA gene and gyrB of the strain with the type strains of Gilvimarinus speices were no higher than 97.9% and 78.7%, respectively, suggesting that the strain is distinct...
September 29, 2016: International Journal of Systematic and Evolutionary Microbiology
Daniel A Russell, Carlos A Guerrero Bustamante, Rebecca A Garlena, Graham F Hatfull
Here, we report the complete genome sequence of Gordonia terrae 3612, also known by the strain designations ATCC 25594, NRRL B-16283, and NBRC 100016. The genome sequence reveals it to be free of prophage and clustered regularly interspaced short palindromic repeats (CRISPRs), and it is an effective host for the isolation and characterization of Gordonia bacteriophages.
September 29, 2016: Genome Announcements
Sooyeon Park, Yong-Taek Jung, Sona Kim, Jung-Hoon Yoon
A Gram-stain-negative, facultative aerobic, non-flagellated, and rod-shaped bacterium, designated AR-13(T), was isolated from a seawater on the East Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain AR-13(T) grew optimally at 30°C, at pH 7.0-8.0 and in the presence of 0-0.5% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain AR-13(T) fell within the clade comprising the type strains of Arcobacter species, clustering coherently with the type strain of Arcobacter venerupis...
October 2016: Journal of Microbiology / the Microbiological Society of Korea
Peng Bao, Ke-Qing Xiao, Hui-Jiao Wang, Hao Xu, Peng-Peng Xu, Yan Jia, Max M Häggblom, Yong-Guan Zhu
A novel nitrate- and selenite reducing bacterium strain ZYK(T) was isolated from a rice paddy soil in Dehong, Yunnan, China. Strain ZYK(T) is a facultative anaerobe and grows in up to 150, 000 ppm O2. The comparative genomics analysis of strain ZYK(T) implies that it shares more orthologues with B. subtilis subsp. subtilis NCIB 3610(T) (ANIm values, 85.4-86.7%) than with B. azotoformans NBRC 15712(T) (ANIm values, 84.4-84.7%), although B. azotoformans NBRC 15712(T) (96.3% 16S rRNA gene sequence similarity) is the closest Bacillus species according to 16S rRNA gene comparison...
September 28, 2016: Scientific Reports
Takahiro Ogawa, Hiromi Kato, Mitsuru Higashide, Mami Nishimiya, Yoko Katayama
Carbonyl sulfide (COS) is an atmospheric trace gas and one of the sources of stratospheric aerosol contributing to climate change. Although one of the major sinks of COS is soil, the distribution of COS degradation ability among bacteria remains unclear. Seventeen out of 20 named bacteria belonging to Actinomycetales had COS degradation activity at mole fractions of 30 parts per million by volume (ppmv) COS. Dietzia maris NBRC 15801(T) and Mycobacterium sp. THI405 had the activity comparable to a chemolithoautotroph Thiobacillus thioparus THI115 that degrade COS by COS hydrolase for energy production...
September 25, 2016: FEMS Microbiology Letters
Chongxi Liu, Lan Ye, Yao Li, Shanwen Jiang, Hui Liu, Kai Yan, Wensheng Xiang, Xiangjing Wang
A phoslactomycins-producing actinomycete, designated strain NEAU-ML8T, was isolated from a millipede (Kronopolites svenhedind Verhoeff) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ML8T belongs to the genus Streptomyces with the highest sequence similarities to Streptomyces lydicus NBRC 13058T (99.39 %) and Streptomyces chattanoogensis DSM 40002T (99.25 %). The maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with S...
September 25, 2016: International Journal of Systematic and Evolutionary Microbiology
Minenosuke Matsutani, Hideki Hirakawa, Eri Hiraoka, Gunjana Theeragool, Toshiharu Yakushi, Kazunobu Matsushita
Acetobacter pasteurianus SKU1108 is a typical thermotolerant acetic acid bacterium. In this study, the complete genome sequence of the SKU1108 strain was elucidated, and information on genomic modifications due to the thermal adaptation of SKU1108 was updated. In order to obtain a clearer understanding of the genetic background responsible for thermotolerance, the SKU1108 genome was compared with those of two closely related complete genome strains, thermotolerant A. pasteurianus 386B and mesophilic A. pasteurianus NBRC 3283...
September 24, 2016: Microbes and Environments
Osamu Yamada, Masayuki Machida, Akira Hosoyama, Masatoshi Goto, Toru Takahashi, Taiki Futagami, Youhei Yamagata, Michio Takeuchi, Tetsuo Kobayashi, Hideaki Koike, Keietsu Abe, Kiyoshi Asai, Masanori Arita, Nobuyuki Fujita, Kazuro Fukuda, Ken-Ichi Higa, Hiroshi Horikawa, Takeaki Ishikawa, Koji Jinno, Yumiko Kato, Kohtaro Kirimura, Osamu Mizutani, Kaoru Nakasone, Motoaki Sano, Yohei Shiraishi, Masatoshi Tsukahara, Katsuya Gomi
Awamori is a traditional distilled beverage made from steamed Thai-Indica rice in Okinawa, Japan. For brewing the liquor, two microbes, local kuro (black) koji mold Aspergillus luchuensis and awamori yeast Saccharomyces cerevisiae are involved. In contrast, that yeasts are used for ethanol fermentation throughout the world, a characteristic of Japanese fermentation industries is the use of Aspergillus molds as a source of enzymes for the maceration and saccharification of raw materials. Here we report the draft genome of a kuro (black) koji mold, A...
September 20, 2016: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
Yong-Guang Zhang, Xin-Hua Lu, Yan-Bo Ding, Xing-Kui Zhou, Hong-Fei Wang, Jian-Wei Guo, Yong-Hong Liu, Yan-Qing Duan, Wen-Jun Li
An alkalitolerant actinomycete strain, designated EGI 80674T, was isolated from a rhizosphere soil of Halocnermum strobilaceum (Pall.) Bieb of Xinjiang, north-west China and subjected to a polyphasic taxonomic characterization. Strain EGI 80674T formed white-colored aerial hyphae with long spore chains. Whole-cell hydrolysates of the isolate contained meso-diaminopimelic acid as the diagnostic diamino acid with no diagnostic sugars. The major fatty acids identified were iso-C16:0, anteiso-C17:0 and 10-methyl-C18:0TBSA...
September 8, 2016: International Journal of Systematic and Evolutionary Microbiology
Yudai Matsuda, Taiki Iwabuchi, Takayuki Fujimoto, Takayoshi Awakawa, Yu Nakashima, Takahiro Mori, Huiping Zhang, Fumiaki Hayashi, Ikuro Abe
Paraherquonin (1), a fungal meroterpenoid produced by Penicillium brasilianum NBRC 6234, possesses a unique, highly congested hexacyclic molecular architecture. Here we identified the biosynthetic gene cluster of 1 (the prh cluster) and elucidated the pathway up to berkeleydione (2), which serves as the key intermediate for the biosynthesis of 1 as well as many other meroterpenoids. Interestingly, the nonheme iron and α-ketoglutarate-dependent dioxygenase PrhA constructs the cycloheptadiene moiety to afford 2 from preaustinoid A1 (6), probably via the homoallyl-homoallyl radical rearrangement...
September 28, 2016: Journal of the American Chemical Society
Chokchai Kittiwongwattana, Chitti Thawai
A bacterium designated as strain roo10T was isolated from roots of Jerusalem artichoke (Helianthus tuberosus). Cells are Gram-stain-negative and non-flagellated rods. The phylogenetic analysis indicated that it was a member of the genus Pseudoxanthomonas, and its close relatives included Pseudoxanthomonas kalamensis JA40T (97.8 % 16S rRNA gene sequence similarity), Pseudoxanthomonas sangjuensis 5GH38-5T (97.7 %) and Pseudoxanthomonas daejeonensis TR6-08T (97.1 %). Growth of strain roo10T occurred at pH ranging from 7 to 9...
August 30, 2016: International Journal of Systematic and Evolutionary Microbiology
Zhi-Qiang Wen, Bingzhi Chen, Xiao Li, Bing-Bing Li, Cheng-Huan Li, Qing-Hua Huang, Qi-Hui Zhang, Wei-Hao Dai, Yu-Ji Jiang
A novel actinomycete strain, designated Js-1T, was isolated from Tremella fuciformis collected from Gutian, Fujian Province, in southeastern China. The taxonomic status of this strain was determined by a polyphasic approach, which demonstrated that the novel strain was a member of the genus Streptomyces. The cell walls of this strain were found to contain LL-diaminopimelic acid, muramic acid, and glycine. An analysis of whole-cell hydrolysates revealed that no characteristic sugar was present. The key identified menaquinones were MK-9 (H6) and MK-9 (H8), while the diagnostic polar lipids were phosphatidyl ethanolamine, diphosphatidyl glycerol, phosphatidyl methyl ethanolamine, and phosphatidyl glycerol...
August 30, 2016: International Journal of Systematic and Evolutionary Microbiology
Zhen-Xing Xu, Qi-Yun Liang, De-Chen Lu, Guan-Jun Chen, Zong-Jun Du
A novel Gram-stain negative, non-motile, moderately halophilic, facultatively anaerobic and spherical bacterium designated strain SS9(T) was isolated from the gill homogenate of a shark. Cells of SS9(T) were observed to be 0.8-1.2 μm in diameter. The strain was found to grow optimally at 33 °C, pH 7.0-8.0 and in the presence of 6.0 % (w/v) NaCl. On the basis of 16S rRNA gene phylogeny, strain SS9(T) can be affiliated with the family Halomonadaceae and is closely related to Chromohalobacter marismortui NBRC 103155(T) (95...
August 26, 2016: Antonie Van Leeuwenhoek
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