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Bo Zhang, Zhi-Qiang Liu, Yu-Guo Zheng
A Gram-stain-negative, aerobic, rod-shaped, bacterium, C62-2T, was isolated from activated sludge in Fujian Province, China. Phylogenetic analysis of the 16S rRNA gene sequences showed that it was closely related to Pedobacter duraquae WB 2.1-25T (97.92 %), Pedobacter bambusae THG-G118T (97.40 %), Pedobacter cryoconitis A37T (97.37 %) and Pedobacter caeni LMG 22862T (97.3 %). Cells grew aerobically at 20-37 °C (optimum, 30 °C), pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-3.0 % (w/v) NaCl...
December 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Hiroshi Toda, Nobuya Itoh
The novel cryptic pKPAL3 plasmid was isolated from the Gram-positive microorganism Kocuria palustris IPUFS-1 and characterized in detail. pKPAL3 is a circular plasmid that is 4,443 bp in length. Open reading frame (ORF) and homology search analyses indicated that pKPAL3 possesses four ORFs; however, there were no replication protein coding genes predicted in the plasmid. Instead, there were two nucleotide sequence regions that showed significant identities with untranslated regions of K. rhizophila DC2201 (NBRC 103217) genomic sequences, and these sequences were essential for autonomous replication of pKPAL3 in Kocuria cells...
2017: Frontiers in Microbiology
Ram Hari Dahal, Jaisoo Kim
Two isolates of bacterial strains A15T and A17 were isolated from stream bank soil in Kyonggi University. Cells were aerobic, Gram-stain-negative, oxidase- and catalase-positive, motile, non-spore-forming, rod-shaped, opaque, and cream coloured. Both strains hydrolysed CM-cellulose. Strains were able to grow at 20-42 °C, pH 5.5-10.0 and at 1.5 % NaCl concentration (w/v). Indole test was positive. Analyses of phylogenetic trees based on its 16S rRNA gene sequences indicated that strain A15T formed a lineage within the family Rhodospirillaceae of the phylum Proteobacteria which was distinct from Ferrovibrio denitrificans S3T (98...
December 8, 2017: International Journal of Systematic and Evolutionary Microbiology
Masahito Hashimoto, Taichi Matsumoto, Miwa Tamura-Nakano, Mami Ozono, Shuhei Hashiguchi, Yasuo Suda
Acetobacter pasteurianus is characterized as a fermenting bacterium of kurozu, which is a common traditional Japanese black vinegar. Recently, we separated acid-resistant and low Toll-like receptor 4 (TLR4)-stimulatory lipopolysaccharides (LPS) from A. pasteurianus. We also showed that their lipid A parts possessed a novel sugar backbone that is responsible for the low TLR4-stimulatory and acid-resistant properties of the LPS. Outer membrane vesicles (OMVs) are nano-sized spherical structures secreted from many gram-negative bacteria...
December 2, 2017: Journal of Bioscience and Bioengineering
Gyu-Min Choi, Wan-Taek Im
A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated NF2-5-3T, was isolated from a paddy soil in Anseong city, Republic of Korea. This bacterium was characterized to determine its taxonomic position using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NF2-5-3T had a close relationship with, and was related most closely to, members of the genus Paraburkholderia, namely Paraburkholderia caribensis MWAP64T (98.8 % similarity), P. sabiae Br3407T (98...
November 29, 2017: International Journal of Systematic and Evolutionary Microbiology
Min Xiao, Nimaichand Salam, Lan Liu, Jian-Yu Jiao, Min-Ling Zheng, Juan Wang, Song Li, Cha Chen, Wen-Jun Li, Ping-Hua Qu
AFrancisella-like bacterium, designated strain SYSU HZH-2T, was isolated from a water sample collected from Haizhu Lake, Guangzhou, China. The bacterium was fastidious, and required an exogenous source of l-cysteine for its growth on artificial media. Cells were Gram-stain-negative, coccobacilli, non-motile and non-spore-forming. The strain shared highest 16S rRNA gene sequence similarities with Cysteiniphilum litorale SYSU D3-2T (94.6 % identity), Fangia hongkongensis UST040201-002T (93.2 %) and Caedibacter taeniospiralis 51T (91...
November 29, 2017: International Journal of Systematic and Evolutionary Microbiology
Nantawan Niemhom, Chitti Thawai
A novel actinomycete strain PS42-9(T), which formed short chains of spores borne on the tip of long sporophores arising from the substrate mycelium, was isolated from soil in Phu-Sang National Park, Phayao province, Thailand. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS42-9(T) were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive glycophospholipids...
November 21, 2017: International Journal of Systematic and Evolutionary Microbiology
N Khebizi, H Boudjella, C Bijani, N Bouras, H-P Klenk, F Pont, F Mathieu, N Sabaou
An actinobacterial strain, HG29, with potent activity against pathogenic, toxigenic and phytopathogenic fungi was isolated from a Saharan soil sample of Algeria. On the basis of morphological and chemotaxonomic characteristics, the strain was classified in the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99.3% with Streptomyces gancidicus NBRC 15412(T). The comparison of its cultural and physiological characteristics with this species revealed significant differences...
November 17, 2017: Journal de Mycologie Médicale
Jaewoo Yoon, Mina Yasumoto-Hirose, Hiroaki Kasai
A polyphasic taxonomic study was performed on a novel strain designated as 04OKA-3-121(T), which was isolated from the hard coral Galaxea fascicularis L. collected at Akajima, Okinawa, Japan. These bacterial cells were observed to be pale-yellow, Gram-stain-negative, strictly aerobic, chemoheterotrophic, non-spore forming, non-motile, and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel marine isolate is affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it shared the highest (93...
November 20, 2017: Current Microbiology
Misugi Uraji, Haruka Tamura, Eiichi Mizohata, Jiro Arima, Kun Wan, Ken'ichi Ogawa, Tsuyoshi Inoue, Tadashi Hatanaka
Feruloyl esterases (FAEs) are key enzymes required for the production of ferulic acid from agricultural biomass. Previously, we identified and characterized R18, an FAE from Streptomyces cinnamoneus NBRC 12852, which showed no sequence similarity with the known FAEs. To determine the region involved in its catalytic activity, we constructed chimeric enzymes using R18 and its homolog (TH2-18) from S. cinnamoneus strain TH-2. Although R18 and TH2-18 showed 74% identity in their primary sequence, the recombinant proteins of these two FAEs (rR18 and rTH2-18) showed very different specific activities toward ethyl ferulate...
November 17, 2017: Applied and Environmental Microbiology
Sooyeon Park, Jeehyun Choi, Su Jung Choi, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile, non-spore-forming bacterial strain, designated JDTF-31(T), was isolated from a tidal flat in Jindo, a South Korean island. Strain JDTF-31(T) grew optimally at 25 °C and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain JDTF-31(T) fell within the cluster comprising the type strains of Tenacibaculum species, joining the type strain of Tenacibaculum soleae. The novel strain exhibited 16S rRNA gene sequence similarity values of 98...
November 17, 2017: International Journal of Systematic and Evolutionary Microbiology
Leonardo Jose Silva, Danilo Tosta Souza, Diego Bonaldo Genuario, Harold Alexander Vargas Hoyos, Suikinai Nobre Santos, Luiz Henrique Rosa, Tiago Domingues Zucchi, Itamar Soares Melo
A novel actinobacterium, designated strain CMAA 1533(T), was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533(T) was found to grow over a wide range of temperatures (4-28 °C) and pH (4-10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533(T) belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus...
November 15, 2017: Antonie Van Leeuwenhoek
Dhiraj Kumar Chaudhary, Ram Hari Dahal, Jaisoo Kim
Two novel strains, J116-2(T) and J116-1, were isolated from forest soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow-coloured, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. The strains were non-sporulating, catalase-positive and oxidase-negative. Strains J116-2(T) and J116-1 were able to grow at 20-32 °C, pH 6.0-8.5, and 0-0.5 % (w/v) NaCl concentration. Based on 16S rRNA gene sequence analyses, strains J116-2(T) and J116-1 formed a distinct lineage within the family Chitinophagaceae of the phylum Bacteroidetes and were closely related to genera Taibaiella (89...
November 15, 2017: International Journal of Systematic and Evolutionary Microbiology
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, Lei Zhao, Min-Kyu Kim, Jae-Hyung Ahn, Jong-Ok Ka
A gamma radiation-resistant and pink-to-red pigmented bacterial strain, designated ID1504(T), was isolated from a car air-conditioning system sampled in Korea. The cells were observed to be Gram-stain negative, aerobic, motile with peritrichous flagella and short rod-shaped. Phylogenetically, the strain groups with the members of the genus Deinococcus and exhibits high 16S rRNA gene sequence similarities with Deinococcus arenae SA1(T) (94.0%), Deinococcus actinosclerus BM2(T) (93.9%) and Deinococcus soli N5(T) (93...
November 14, 2017: Antonie Van Leeuwenhoek
Peng Ding, Juan-Luan Bai, Tian-Tian Wang, Yong Sun, Cheng-Liang Cao, Ji-Hong Jiang, Sheng Qin
An actinomycete strain, designated KLBMP S0039(T), was isolated from the rhizosphere soil of Lycium Linn., collected from the coastal region in Lianyungang, Jiangsu Province, eastern PR China, and was studied to determine its taxonomic position. The isolate showed a combination of morphological and chemotaxonomic properties typical of the members of the genus Nocardia. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were galactose, arabinose, glucose and ribose...
November 10, 2017: International Journal of Systematic and Evolutionary Microbiology
Shanwen Jiang, Chenyu Piao, Yang Yu, Peng Cao, Chenxu Li, Fan Yang, Mutong Li, Wensheng Xiang, Chongxi Liu
A novel actinomycete, designated strain 1H-SSA4(T), was isolated from the head of an ant (Camponotus japonicus Mayr) and was found to produce angucyclinone antibiotics. A polyphasic approach was used to determine the taxonomic status of strain 1H-SSA4(T). The DNA G+C content of the draft genome sequence, consisting of 11.4 Mbp, was 70.0 mol%. 16S rRNA gene sequence similarity studies showed that strain 1H-SSA4(T) belongs to the genus Streptomyces with the highest sequence similarity to Streptomyces hygroscopicus subsp...
November 7, 2017: International Journal of Systematic and Evolutionary Microbiology
Marilize le Roes-Hill, Alaric Prins, Paul R Meyers
As part of an antibiotic screening program, an actinobacterium, strain HMC13(T), was isolated from soil collected from the banks of the Gamka River, Western Cape Province, South Africa. The isolate was found to produce branched mycelia that differentiated into spiral spore chains with spiny spores. 16S rRNA gene sequence analysis showed the strain to be closely related to Streptomyces caelestis NRRL 2418(T) (99.72%) and Streptomyces azureus NBRC 12744(T) (99.51%). Chemotaxonomic analyses confirmed the classification of the strain as a member of the genus Streptomyces: LL-DAP in the peptidoglycan, no diagnostic sugars in the whole cell sugar pattern, dominant menaquinones including MK9(H8), MK9(H6), and the polar lipids detected included phosphatidylethanolamine...
November 6, 2017: Antonie Van Leeuwenhoek
Ahmad Suparmin, Tatsuya Kato, Hideo Dohra, Enoch Y Park
Cordyceps militaris produces cordycepin, which is known to be a bioactive compound. Currently, cordycepin hyperproduction of C. militaris was carried out in a liquid surface culture because of its low productivity in a submerged culture, however the reason was not known. In this study, 4.92 g/L of cordycepin was produced at the 15th day of C. militaris NBRC 103752 liquid surface culture, but only 1 mg/L was produced in the submerged culture. RNA-Seq was used to clarify the gene expression profiles of the cordycepin biosynthetic pathways of the submerged culture and the liquid surface culture...
2017: PloS One
Guang-Da Feng, Xiong Xiong, Hong-Hui Zhu, Hua-Ping Li
A difficult to cultivate bacterial strain, designated 1PNM-26(T), isolated from a lead-zinc mine, was investigated using a polyphasic taxonomic approach. The strain was able to grow on solid medium but not in liquid medium. Cells were Gram-reaction-negative, aerobic, non-spore-forming, non-motile and rod-shaped. It showed positive reactions for catalase and oxidase and hydrolysis of aesculin. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 1PNM-26(T) represents a member of the genus Sphingomonas and forms a stable cluster with Sphingomonas morindae KCTC 42183(T), Sphingomonas polyaromaticivorans JCM 16711(T) and Sphingomonas oligoaromativorans NBRC 105508(T)...
November 1, 2017: International Journal of Systematic and Evolutionary Microbiology
Juan Li, Chisaki Kudo, Akio Tonouchi
A bacterial strain designated PtRA-8(T) was isolated from the trunk surface of a Japanese aspen tree (Populus tremula var. sieboldii). Cells of strain PtRA-8(T) were aerobic, non-motile, non-spore forming, Gram-stain-negative rods, 1.0‒2.0 µm in width and 3.0‒10.0 µm in length. The pH range for growth was between 5.5 and 7.5, with an optimum at 6.5. The temperature range for growth was between 10 and 37 °C, with an optimum at around 25‒30 °C. Strain PtRA-8(T) was highly resistant to UV irradiation, similar to its Deinococcus relatives...
October 28, 2017: Archives of Microbiology
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