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https://www.readbyqxmd.com/read/29049338/growing-plants-on-oily-nutrient-poor-soil-using-a-native-symbiotic-fungus
#1
Timothy S Repas, D Michael Gillis, Zakia Boubakir, Xiaohui Bao, Gary J Samuels, Susan G W Kaminskyj
The roots of land plants associate with microbes, including fungal symbionts that can confer abiotic stress tolerance. Bitumen extraction following oil-sand surface mining in the Athabasca region of Alberta, Canada removes plant nutrients but leaves a petrochemical residue, making the coarse tailings (CT) hostile to both plants and microbes. We isolated an endophyte strain of the Ascomycete Trichoderma harzianum we call TSTh20-1 (hereafter, TSTh) from a dandelion that was naturally growing on CT. TSTh colonization allowed tomato, wheat, and remediation seed mixtures to germinate and their seedlings to flourish on CT without the use of fertilizer...
2017: PloS One
https://www.readbyqxmd.com/read/29046282/clonality-in-context-hematopoietic-clones-in-their-marrow-environment
#2
James N Cooper, Neal S Young
Clonal hematopoiesis occurs normally, especially with aging, and in the setting of disease, not only myeloid cancers but in bone marrow failure. In cancer, malignant clones are characterized by recurrent somatic mutations in specific sets of genes, but the direct relationship of such mutations to leukemogenesis, when they occur in an apparently healthy older individual or after recovery from immune aplastic anemia, is uncertain. Here we emphasize a view of clonal evolution that stresses natural selection over deterministic ontogeny, and we stress the selective role of the marrow's, and organism's, environment...
October 18, 2017: Blood
https://www.readbyqxmd.com/read/29045922/the-impact-of-expired-commercial-drugs-on-non-target-marine-species-a-case-study-with-the-use-of-a-battery-of-biomarkers-in-hemocytes-of-mussels
#3
Nektarios Politakis, Alexia Belavgeni, Ioanna Efthimiou, Nikolina Charalampous, Chara Kourkouta, Stefanos Dailianis
The present study investigated the effects of two expired commercial medicines, like Buscopan Plus and Mesulid, commonly classified as household medical wastes, on hemocytes of mussel Mytilus galloprovincialis. Mussel hemocytes' lysosomal membrane stability (in terms of neutral red retention assay), superoxide anions (O2(·)(-)) and nitric oxides (NO, in terms of nitrites) production, lipid peroxidation (in terms of malondialdehyde/MDA content) and the formation of nuclear abnormalities (using the micronucleus/MN assay) were assessed in hemocytes of mussels treated for 7 days with appropriate amounts of each drug (the concentrations of active substances were considered in each case, due to the absence of data related with the excipients) as well as in hemocytes of post-treated/recovered mussels (7 days post-treatment/recovery period)...
October 14, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29045327/aging-impairs-whole-body-heat-loss-in-women-under-both-dry-and-humid-heat-stress
#4
Sean R Notley, Martin P Poirier, Stephen G Hardcastle, Andreas D Flouris, Pierre Boulay, Ronald J Sigal, Glen P Kenny
PURPOSE: This study was designed to determine whether age-related impairments in whole-body heat loss, which are known to exist in dry heat, also occur in humid heat in women. METHODS: To evaluate this possibility, 10 young (25 ± 4 yr) and 10 older (51 ± 7 yr) women matched for body surface area (young, 1.69 ± 0.11; older, 1.76 ± 0.14 m, P = 0.21) and peak oxygen consumption (V˙O2peak) (young, 38.6 ± 4.6; older, 34.8 ± 6.6 mL·kg·min, P = 0.15) performed four 15-min bouts of cycling at a fixed metabolic heat production rate (300 W; equivalent to ~45% V˙O2peak), each separated by a 15-min recovery, in dry (35°C, 20% relative humidity) and humid heat (35°C, 60% relative humidity)...
November 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29043555/impact-of-non-specific-normal-databases-on-perfusion-quantification-of-low-dose-myocardial-spect-studies
#5
Camilla Scabbio, Orazio Zoccarato, Simona Malaspina, Giovanni Lucignani, Angelo Del Sole, Michela Lecchi
AIM: To evaluate the impact of non-specific normal databases on the percent summed rest score (SR%) and stress score (SS%) from simulated low-dose SPECT studies by shortening the acquisition time/projection. METHODS: Forty normal-weight and 40 overweight/obese patients underwent myocardial studies with a conventional gamma-camera (BrightView, Philips) using three different acquisition times/projection: 30, 15, and 8 s (100%-counts, 50%-counts, and 25%-counts scan, respectively) and reconstructed using the iterative algorithm with resolution recovery (IRR) Astonish(TM) (Philips)...
October 17, 2017: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/29042653/silica-precipitation-potentially-controls-earthquake-recurrence-in-seismogenic-zones
#6
Hanae Saishu, Atsushi Okamoto, Makoto Otsubo
Silica precipitation is assumed to play a significant role in post-earthquake recovery of the mechanical and hydrological properties of seismogenic zones. However, the relationship between the widespread quartz veins around seismogenic zones and earthquake recurrence is poorly understood. Here we propose a novel model of quartz vein formation associated with fluid advection from host rocks and silica precipitation in a crack, in order to quantify the timescale of crack sealing. When applied to sets of extensional quartz veins around the Nobeoka Thrust of SW Japan, an ancient seismogenic splay fault, our model indicates that a fluid pressure drop of 10-25 MPa facilitates the formation of typical extensional quartz veins over a period of 6...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042194/knockdown-of-rmi1-impairs-dna-repair-under-dna-replication-stress
#7
Chang Xu, Lianying Fang, Yangyang Kong, Changyan Xiao, Mengmeng Yang, Li-Qing Du, Qiang Liu
RMI1 (RecQ-mediated genome instability protein 1) forms a conserved BTR complex with BLM, Topo IIIα, and RMI2, and its absence causes genome instability. It has been revealed that RMI1 localizes to nuclear foci with BLM and Topo IIIα in response to replication stress, and that RMI1 functions downstream of BLM in promoting replication elongation. However, the precise functions of RMI1 during replication stress are not completely understood. Here we report that RMI1 knockdown cells are hypersensitive to hydroxyurea (HU)...
October 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29041955/pluripotent-nontumorigenic-multilineage-differentiating-stress-enduring-cells-muse-cells-a-seven-year-retrospective
#8
REVIEW
Samantha C Fisch, María L Gimeno, Julia D Phan, Ariel A Simerman, Daniel A Dumesic, Marcelo J Perone, Gregorio D Chazenbalk
Multilineage differentiating stress enduring (Muse) cells, discovered in the spring of 2010 at Tohoku University in Sendai, Japan, were quickly recognized by scientists as a possible source of pluripotent cells naturally present within mesenchymal tissues. Muse cells normally exist in a quiescent state, singularly activated by severe cellular stress in vitro and in vivo. Muse cells have the capacity for self-renewal while maintaining pluripotent cell characteristics indicated by the expression of pluripotent stem cell markers...
October 18, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29041005/cardiogenic-shock-during-first-infusion-of-anthracycline-chemotherapy-in-a-patient-with-hodgkin-lymphoma-an-unusual-event
#9
Giulio Binaghi, Damiana Congia, Stefano Cossa, Stefania Massidda, Daniele Pasqualucci, Federica Pilo, Emanuela Serra, Emanuele Angelucci, Maurizio Porcu
INTRODUCTION: Hodgkin lymphoma (HL) is one of the most common types of cancers of the lymphatic system. The currently available therapies enable a cure in approximately 80-85% of treated patients. However, the cardiotoxicity of HL treatment has become a major cause of morbidity and mortality in survivors mainly related to the use of anthracycline. CASE REPORT: An HL, staged IIIB, was diagnosed in a 60-year-old man with no cardiovascular disease. During the first cycle of ABVD chemotherapy (Adriamycin; bleomycin; vinblastine; dacarbazine), near the end of the dacarbazine infusion, the patient presented a sudden cardiogenic shock characterized by a severe left ventricular systolic dysfunction...
October 18, 2017: Cardiology
https://www.readbyqxmd.com/read/29040771/contrasting-ecophysiological-strategies-related-to-drought-the-case-of-a-mixed-stand-of-scots-pine-pinus-sylvestris-and-a-submediterranean-oak-quercus-subpyrenaica
#10
Paula Martín-Gómez, Mònica Aguilera, Jesús Pemán, Eustaquio Gil-Pelegrín, Juan Pedro Ferrio
Submediterranean forests are considered an ecotone between Mediterranean and Eurosiberian ecosystems, and are very sensitive to global change. A decline of Scots pine (Pinus sylvestris L.) and a related expansion of oak species (Quercus spp.) have been reported in the Spanish Pre-Pyrenees. Although this has been associated with increasing drought stress, the underlying mechanisms are not fully understood, and suitable monitoring protocols are lacking. The aim of this study is to bring insight into the physiological mechanisms anticipating selective decline of the pines, with particular focus on carbon and water relations...
October 13, 2017: Tree Physiology
https://www.readbyqxmd.com/read/29038678/posttraumatic-growth-and-recovery-from-addiction
#11
Eyal Haroosh, Sara Freedman
Background: It is well documented that individuals coping with adverse events report both negative outcomes, such as posttraumatic stress symptoms, as well as positive changes, described as posttraumatic growth. Positive changes are also reported in people who have recovered from substance abuse. It seems plausible from the literature that both of these types of positive changes have elements in common. To date, no published studies have examined positive outcomes among people who have recovered from addiction...
2017: European Journal of Psychotraumatology
https://www.readbyqxmd.com/read/29038164/pka-creb-signaling-prevents-adriamycin-induced-podocyte-apoptosis-via-upregulation-of-mitochondrial-respiratory-chain-complexes
#12
Kewei Xie, Mingli Zhu, Peng Xiang, Xiaohuan Chen, Ayijiaken Kasimumali, Renhua Lu, Qin Wang, Shan Mou, Zhaohui Ni, Leyi Gu, Huihua Pang
Previous work showed that the activation of protein kinase A (PKA) signaling promoted mitochondrial fusion and prevented podocyte apoptosis. The cAMP response element binding protein (CREB) is the main downstream transcription factor of PKA signaling. Herein, we show that the pKA-agonist pCPT-cAMP prevented the production of adriamycin (ADR)-induced reactive oxygen species and apoptosis in podocytes, which were inhibited by CREB RNAi. The activation of PKA enhanced mitochondrial function and prevented the ADR-induced decrease of mitochondrial respiratory chain complex I subunits, NADH-ubiquinone oxidoreductase complex (ND) 1/3/4 genes, and protein expression...
October 16, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29038013/a-transcriptomics-assessment-of-oxygen-temperature-interactions-reveals-novel-candidate-genes-underlying-variation-in-thermal-tolerance-and-survival
#13
Leigh Boardman, Katherine A Mitchell, John S Terblanche, Jesper G Sørensen
While single stress responses are fairly well researched, multiple, interactive stress responses are not -despite the obvious importance thereof. Here, using D. melanogaster, we investigated the effects of simultaneous exposures to low O2 (hypoxia) and varying thermal conditions on mortality rates, estimates of thermal tolerance and the transcriptome. We used combinations of 21 (normoxia), 10 or 5 kPa O2 with control (23°C), cold (4°C) or hot (31°C) temperature exposures before assaying chill coma recovery time (CCRT) and heat knock down time (HKDT) as measures of cold and heat tolerance respectively...
October 13, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29037399/comparison-of-heat-strain-recovery-in-different-anti-heat-stress-clothing-ensembles-after-work-to-exhaustion
#14
Yijie Zhao, Wen Yi, Albert P C Chan, Daniel W M Chan
A hot environment combined with physically demanding tasks can subject workers to a higher risk of heat stress. A series of regulations and guidelines have been proposed to design appropriate anti-heat stress work uniform to reduce body heat strain. The present study aimed to examine heat strain recovery in different anti-heat stress clothing ensembles after work to exhaustion in the heat. 10 healthy males performed intermittent treadmill running/walking to exhaustion, followed by 30min passive recovery sitting in a climatic chamber, which simulated the hot and humid outdoor environment (34°C temperature, 60% relative humidity, 0...
October 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29037397/development-of-the-embryonic-heat-shock-response-and-the-impact-of-repeated-thermal-stress-in-early-stage-lake-whitefish-coregonus-clupeaformis-embryos
#15
Lindy M Whitehouse, Chance S McDougall, Daniel I Stefanovic, Douglas R Boreham, Christopher M Somers, Joanna Y Wilson, Richard G Manzon
Lake whitefish (Coregonus clupeaformis) embryos were exposed to thermal stress (TS) at different developmental stages to determine when the heat shock response (HSR) can be initiated and if it is altered by exposure to repeated TS. First, embryos were subject to one of three different TS temperatures (6, 9, or 12°C above control) at 4 points in development (21, 38, 60 and 70 days post-fertilisation (dpf)) for 2h followed by a 2h recovery to understand the ontogeny of the HSR. A second experiment explored the effects of repeated TS on the HSR in embryos from 15 to 75 dpf...
October 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29036865/differences-in-low-back-pain-occurrence-over-a-6-month-period-between-four-recovery-stress-groups
#16
Tobias Mierswa, Michael Kellmann
BACKGROUND: Psychosocial work factors and general recovery experiences are hypothesised to influence the risk of low back pain (LBP) occurrence by influencing the acute recovery-stress state. So far, however, direct links between the acute recovery-stress state and LBP occurrence have not been investigated in detail. OBJECTIVE: The aim of this study was to investigate differences in low back pain occurrence between four distinct recovery-stress groups over a period of 6 month...
October 8, 2017: Work: a Journal of Prevention, Assessment, and Rehabilitation
https://www.readbyqxmd.com/read/29035591/effect-of-intensified-endurance-training-on-pacing-and-performance-in-4000m-cycling-time-trials
#17
Alice M Wallett, Amy L Woods, Nathan G Versey, Laura A Garvican-Lewis, Marijke Welvaert, Kevin G Thompson
Studies examining pacing strategies during 4000m cycling time-trials (TTs) typically ensure participants are not pre-fatigued, however competitive cyclists often undertake TTs when already pre-fatigued. This study aimed to determine how TT pacing strategies and sprint characteristics of cyclists change during an intensified training period (mesocycle). Thirteen cyclists, regularly competing in A and B grade cycling races and consistently training (>10 h·wk-1 for 4±1 years), completed a six-week training mesocycle...
October 16, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/29034471/lipid-signaling-affects-primary-fibroblast-collective-migration-and-anchorage-in-response-to-stiffness-and-microtopography
#18
Michael A Mkrtschjan, Snehal B Gaikwad, Kevin J Kappenman, Christopher Solís, Sagar Dommaraju, Long Le, Tejal A Desai, Brenda Russell
Cell migration is regulated by several mechanotransduction pathways, which consist of sensing and converting mechanical microenvironmental cues to internal biochemical cellular signals, such as protein phosphorylation and lipid signaling. While there has been significant progress in understanding protein changes in the context of mechanotransduction, lipid signaling is more difficult to investigate. In this study, physical cues of stiffness (10 kPa, 100 kPa, 400 kPa, and glass), and microrod or micropost topography were manipulated in order to reprogram primary fibroblasts and assess the effects of lipid signaling on the actin cytoskeleton...
October 15, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29033925/caco-2-invasion-by-cronobacter-sakazakii-and-salmonella-enterica-exposed-to-drying-and-heat-treatments-in-dried-state-in-milk-powder
#19
Emilie Lang, Stéphane Guyot, Pablo Alvarez-Martin, Jean-Marie Perrier-Cornet, Patrick Gervais
Due to the ability of foodborne pathogens to survive in low moisture food, the decontamination of milk powder is an important issue in food protection. The safety of food products is, however, not always insured and the different steps in the processing of food involve physiological and metabolic changes in bacteria. Among these changes, virulence properties may also be affected. In this study, the effect of drying and successive thermal treatments on the invasion capacity of Salmonella Typhimurium, Salmonella Senftenberg, and Cronobacter sakazakii was assessed...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29033512/heart-rate-recovery-as-a-novel-test-for-predicting-cardiac-involvement-in-beta-thalassemia-major
#20
Selcuk Kucukseymen, Isa Oner Yuksel, Goksel Cagirci, Erkan Koklu, Volkan Karakus, Serkan Cay, Gorkem Kus, Erdal Kurtoglu, Sakir Arslan
BACKGROUND: Abnormal heart rate recovery (HRR) is predictive of cardiac mortality. Autonomic abnormalities in beta-thalassemia major (TM) patients have been reported in previous studies. However, the importance of low HRR in exercise stress test in TM patients has not yet been ascertained. Therefore, this study will be the first of its kind in the literature. METHODS: Exercise stress test was performed on 56 TM patients who were being treated at the Thalassemia Center of our hospital, along with 46 non-TM iron deficiency anemia (IDA) patients as a control group...
July 2017: Acta Cardiologica Sinica
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