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https://www.readbyqxmd.com/read/28222245/translation-rewiring-at-the-heart-of-phenotype-switching-in-melanoma
#1
Eleonora Leucci, Pierre Close, Jean-Christophe Marine
During tumor progression cancer cells are constantly challenged by intrinsic oncogene-induced and extrinsic micro-environmental stress signals such as hypoxia, nutrient deprivation, oxidative and genotoxic stress. Under these suboptimal growth conditions cancer cells activate an Integrative Stress Response (ISR), which results in a transient decrease in energy consumption followed by a cellular adaptation phase and possibly recovery, if the environmental conditions become more favorable. Translation is a very high energy-consuming process; it is therefore not surprising that to minimize energy consumption cancer cells turn down global protein synthesis rates while reprograming translation towards specific transcripts required for survival and adaptation...
February 21, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/28221856/enumeration-and-identification-of-coliform-bacteria-injured-by-chlorine-or-fungicide-mixed-with-agricultural-water
#2
Hidemi Izumi, Yuji Nakata, Ayano Inoue
Chemical sanitizers may induce no injury (bacteria survive), sublethal injury (bacteria are injured), or lethal injury (bacteria die). The proportion of coliform bacteria that were injured sublethally by chlorine and fungicide mixed with agricultural water (pond water), which was used to dilute the pesticide solution, was evaluated using the thin agar layer (TAL) method. In pure cultures of Enterobacter cloacae , Escherichia coli , and E. coli O157:H7 (representing a human pathogen), the percentage of chlorine-injured cells was 69 to 77% for dilute electrolyzed water containing an available chlorine level of 2 ppm...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28220500/neuroplus-biofeedback-improves-attention-resilience-and-injury-prevention-in-elite-soccer-players
#3
Aiace Rusciano, Giuliano Corradini, Ivilin Stoianov
Performance and injury prevention in elite soccer players are typically investigated from physical-tactical, biomechanical, and metabolic perspectives. However, executive functions, visuospatial abilities, and psychophysiological adaptability or resilience are also fundamental for efficiency and well-being in sports. Based on previous research associating autonomic flexibility with prefrontal cortical control, we designed a novel integrated autonomic biofeedback training method called Neuroplus to improve resilience, visual attention, and injury prevention...
February 21, 2017: Psychophysiology
https://www.readbyqxmd.com/read/28220475/dyadic-flexibility-mediates-the-relation-between-parent-conflict-and-infants-vagal-reactivity-during-the-face-to-face-still-face
#4
Alex Busuito, Ginger A Moore
Parent conflict is related to attenuated infant vagal reactivity, suggesting less effective regulation. Because infants' self-regulation develops in the context of coregulation, the current study examined a novel measure, flexibility, purported to reflect dyadic reorganization in response to contextual demands. Flexibility was expected to mediate the relation between greater conflict and lesser vagal reactivity during the reunion episode of the Face-to-Face Still-Face (FFSF). Six-month-old infants' and their mothers' (N = 53) affective behaviors were observed during the FFSF and heart rate data were collected for infants...
February 21, 2017: Developmental Psychobiology
https://www.readbyqxmd.com/read/28220140/nbs-lrr-protein-pik-h4-interacts-with-osbihd1-to-balance-rice-blast-resistance-and-growth-by-coordinating-ethylene-brassinosteroid-pathway
#5
Hao Liu, Shuangyu Dong, Fengwei Gu, Wei Liu, Guili Yang, Ming Huang, Wuming Xiao, Yongzhu Liu, Tao Guo, Hui Wang, Zhiqiang Chen, Jiafeng Wang
The regulation of innate immunity and plant growth, along with the trade-off between them, affects the defense and recovery mechanisms of the plant after it is attacked by pathogens. Although it is known that hormonal crosstalk plays a major role in regulating interaction of plant growth and PAMP-triggered immunity, the relationship between plant growth and effector-triggered immunity (ETI) remains unclear. In a large-scale yeast two-hybrid screening for Pik-H4-interacting proteins, a homeodomain transcription factor OsBIHD1 was identified, which is previously known to function in biotic and abiotic stress responses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28216637/mutations-underlying-episodic-ataxia-type-1-antagonize-kv1-1-rna-editing
#6
Elizabeth A Ferrick-Kiddie, Joshua J C Rosenthal, Gregory D Ayers, Ronald B Emeson
Adenosine-to-inosine RNA editing in transcripts encoding the voltage-gated potassium channel Kv1.1 converts an isoleucine to valine codon for amino acid 400, speeding channel recovery from inactivation. Numerous Kv1.1 mutations have been associated with the human disorder Episodic Ataxia Type-1 (EA1), characterized by stress-induced ataxia, myokymia, and increased prevalence of seizures. Three EA1 mutations, V404I, I407M, and V408A, are located within the RNA duplex structure required for RNA editing. Each mutation decreased RNA editing both in vitro and using an in vivo mouse model bearing the V408A allele...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28216547/mid-term-follow-up-of-school-aged-children-with-borderline-long-qt-interval
#7
Aya Miyazaki, Heima Sakaguchi, Yu Matsumura, Yosuke Hayama, Kanae Noritake, Jun Negishi, Etsuko Tsuda, Yoshihiro Miyamoto, Takeshi Aiba, Wataru Shimizu, Kengo Kusano, Isao Shiraishi, Hideo Ohuchi
BACKGROUND: There are no definitive diagnostic criteria or follow-up strategies for long QT syndrome (LQTS) in children with a borderline long QT interval (b-LQT).Methods and Results:We retrospectively evaluated the clinical course, genetic testing results, corrected QT interval (QTc), and LQTS score of 59 school-aged children (5-18 years old) with a b-LQT (400≤QTc<500 ms). Syncope, but neither aborted cardiac arrest nor sudden cardiac death, occurred in 2 patients during the follow-up (6±3...
February 18, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28216514/diallyl-trisulfide-augments-ischemia-induced-angiogenesis-via-an-endothelial-nitric-oxide-synthase-dependent-mechanism
#8
Ryo Hayashida, Kazuhisa Kondo, Sumio Morita, Kazumasa Unno, Satoshi Shintani, Yuuki Shimizu, John W Calvert, Rei Shibata, Toyoaki Murohara
BACKGROUND: Hydrogen sulfide (H2S) exerts beneficial actions against the development of cardiovascular disease. Diallyl trisulfide (DATS) is an organic polysulfide found in garlic oil that liberates H2S under physiological conditions. This study investigated whether DATS modulates endothelial cell function, as well as revascularization processes in a mouse model of hind-limb ischemia.Methods and Results:Wild-type (WT), endothelial nitric oxide synthase-deficient (eNOS-KO) and Akt1-heterogenic deficient (Akt-Het) mice were subjected to unilateral hindlimb ischemia (HLI)...
February 17, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28215878/neuroactive-steroids-and-ptsd-treatment
#9
REVIEW
Ann M Rasmusson, Christine E Marx, Suzanne L Pineles, Andrea Locci, Erica R Scioli-Salter, Yael I Nillni, Jennifer J Liang, Graziano Pinna
This review highlights early efforts to translate pre-clinical and clinical findings regarding the role of neuroactive steroids in stress adaptation and PTSD into new therapeutics for PTSD. Numerous studies have demonstrated PTSD-related alterations in resting levels or the reactivity of neuroactive steroids and their targets. These studies also have demonstrated substantial variability in the dysfunction of specific neuroactive steroid systems among PTSD subpopulations. These variabilities have been related to the developmental timing of trauma, severity and type of trauma, genetic background, sex, reproductive state, lifestyle influences such as substance use and exercise, and the presence of comorbid conditions such as depression and chronic pain...
February 12, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28215415/diagnostic-and-prognostic-value-of-lead-avr-during-exercise-testing-in-patients-suspected-of-having-myocardial-ischemia
#10
Max Wagener, Roger Abächerli, Ursina Honegger, Nicolas Schaerli, Gil Prêtre, Raphael Twerenbold, Christian Puelacher, Germaine Sunier, Philipp Reddiess, Maria Rubini Gimenez, Karin Wildi, Jasper Boeddinghaus, Thomas Nestelberger, Patrick Badertscher, Zaid Sabti, Ramun Schmid, Remo Leber, Dayana Flores Widmer, Samyut Shrestha, Ivo Strebel, Damian Wild, Stefan Osswald, Michael Zellweger, Christian Mueller, Tobias Reichlin
We aimed to assess the diagnostic and prognostic value of ST-segment deviation in aVR, a lead often ignored in clinical practice, during exercise testing and to compare it to the most widely used criterion of ST-segment depression in V5. We enrolled 1,596 patients with suspected myocardial ischemia referred for nuclear perfusion imaging undergoing bicycle stress testing. ST-segment amplitudes in leads aVR and V5 were automatically measured. The presence of inducible myocardial ischemia was the diagnostic end point and adjudicated based on nuclear perfusion imaging and coronary angiography...
January 5, 2017: American Journal of Cardiology
https://www.readbyqxmd.com/read/28214551/effect-of-benzalkonium-chloride-on-trabecular-meshwork-cells-in-a-new-in-vitro-3d-trabecular-meshwork-model-for-glaucoma
#11
Meryem Bouchemi, Christophe Roubeix, Karima Kessal, Luisa Riancho, Anne-Laure Raveu, Hayet Soualmia, Christophe Baudouin, Françoise Brignole-Baudouin
PURPOSE: To validate a new culture model of primary human trabecular meshwork cells (p-hTMCs) using Matrigel®, in order to mimic in vitro 3D-TM organization, and to investigate the proinflammatory effect of benzalkonium chloride (BAK) in 3D p-hTMC cultures. METHODS: p-hTMCs, seeded onto Matrigel®-coated inserts were stimulated with BAK (10(-4)%), dexamethasone (DEX) (10(-6)M) or transforming growth factor-beta 2 (TGF-β2) (5ng/ml) for 48h and observed with confocal microscopy...
February 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28214242/injury-and-recovery-of-escherichia-coli-atcc25922-cells-treated-by-high-hydrostatic-pressure-at-400-600%C3%A2-mpa
#12
Keitarou Kimura, Kazuya Morimatsu, Takashi Inaoka, Kazutaka Yamamoto
Escherichia coli cells were inactivated by high hydrostatic pressure (HHP) at 400-600 MPa and their recovery under various conditions was evaluated by colony counting and flow cytometer (FCM) analyses. The lag time in colony formation and an improved recovery of cells under less oxidative conditions (pyruvate addition to the medium and incubation in anaerobic conditions) were observed for HHP treated cells, which indicated that a significant portion of cells were injured and recovered during incubation after HHP treatment...
February 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28213166/influence-of-clamping-stress-and-duration-on-the-trauma-of-liver-tissue-during-surgery-operation
#13
Jin Wang, Qing-Yuan Yu, Wei Li, Bing-Rui Wang, Zhong-Rong Zhou
BACKGROUND: Tissue grasping damage often occurs in minimally invasive surgery, which would increase the postoperative recovery time and the risk of surgical complications. The purpose of this study was to evaluate the relationship between liver tissue trauma and compression stress magnitude and duration during tissue clamping operation. METHODS: The clamping experiments of liver tissues in vivo were conducted by using a universal soft tissue mechanical testing machine under different clamping stress magnitudes and durations...
February 10, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28212641/local-constraints-to-access-appropriate-malaria-treatment-in-the-context-of-parasite-resistance-in-cambodia-a-qualitative-study
#14
Jesse Verschuere, Tom Decroo, Dara Lim, Jean-Marie Kindermans, Chea Nguon, Rekol Huy, Yasmine Alkourdi, Koen Peeters Grietens, Charlotte Gryseels
BACKGROUND: Despite emerging drug resistance in Cambodia, artemisinin-based combination therapy (ACT) is still the most efficacious therapy. ACT is available free of charge in the Cambodian public sector and at a subsidized rate in the private sector. However, un- and mistreated cases in combination with population movements may lead to the further spread of resistant parasites, stressing the importance of understanding how the perceived aetiology of malaria and associated health-seeking behaviour may delay access to appropriate treatment...
February 17, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28212395/delayed-apoptosis-allows-islet-%C3%AE-cells-to-implement-an-autophagic-mechanism-to-promote-cell-survival
#15
Heather L Hayes, Brett S Peterson, Jonathan M Haldeman, Christopher B Newgard, Hans E Hohmeier, Samuel B Stephens
Increased β-cell death coupled with the inability to replicate existing β-cells drives the decline in β-cell mass observed in the progression of both major forms of diabetes. Understanding endogenous mechanisms of islet cell survival could have considerable value for the development of novel strategies to limit β-cell loss and thereby promote β-cell recovery. Insulinoma cells have provided useful insight into β-cell death pathways but observations made in cell lines sometimes fail to translate to primary islets...
2017: PloS One
https://www.readbyqxmd.com/read/28211094/comparison-of-procedural-sequences-in-same-day-painless-bidirectional-endoscopy-a-single-center-prospective-randomized-study
#16
Yan Cao, Jun Yang, Jiao Li, Xin Ao, Kai-Yuan Zhang, Xiao-Chun Shen, Dong-Feng Chen, Chun-Hui Lan
AIMS: To compare the efficacy and safety of esophagogastroduodenoscopy (EGD)-colonoscopy and colonoscopy-EGD sequences for patients subjected to same-day bidirectional endoscopy under remifentanil and propofol sedation. METHOD: A total of 209 eligible outpatients scheduled for diagnostic same-day bidirectional endoscopy between February 16, 2016 and April 30, 2016 were randomly assigned to the EGD-colonoscopy (n=106) and colonoscopy-EGD (n=103) sequence groups. The primary endpoint was the total dose of propofol required for the procedure...
February 17, 2017: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/28210899/interleukin-6-as-possible-early-marker-of-stress-response-after-femoral-fracture
#17
Goran Pesic, Jovana Jeremic, Tamara Nikolic, Vladimir Zivkovic, Ivan Srejovic, Aleksandra Vranic, Jovana Bradic, Branko Ristic, Aleksandar Matic, Nikola Prodanovic, Vladimir Jakovljevic
Bone fracture healing is a complex process which at best results in full recovery of function and structure of injured bone tissue, but all the mechanisms involved in this process, and their mutual interaction, are not fully understood. Despite advancement of surgical procedures, this type of fractures is still a major public health concern. In the last few decades, a lot of attention is focused on the oxygen-free radicals and inflammatory response markers as important factors of skeletal injury. Thus, the aim of the present study was to follow the changes in redox balance and inflammatory response in elderly patients with femoral fractures during the earliest stages of fracture healing, by measuring the values of the observed markers immediately after fracture, as well as the first, third, and seventh postoperative day...
February 16, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28209683/evaluation-of-6-remote-first-nations-community-based-buprenorphine-programs-in-northwestern-ontario-retrospective-study
#18
Solomon Mamakwa, Meldon Kahan, Dinah Kanate, Mike Kirlew, David Folk, Sharon Cirone, Sara Rea, Pierre Parsons, Craig Edwards, Janet Gordon, Fiona Main, Len Kelly
OBJECTIVE: To evaluate established opioid addiction treatment programs that use traditional healing in combination with buprenorphine-naloxone maintenance treatment in 6 First Nations communities in the Sioux Lookout region of northwestern Ontario. DESIGN: Retrospective cohort study. SETTING: Six First Nations communities in northwestern Ontario. PARTICIPANTS: A total of 526 First Nations participants in opioid-dependence treatment programs...
February 2017: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/28208930/precautionary-measures-for-successful-open-heart-surgery-in-g6pd-deficient-patient-a-case-report
#19
Rupesh Kumar
Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is among the most common enzymatic disorders of red blood cells. Cardiac surgeries on this group of individuals are associated with an additional risk in terms of impaired oxygenation, prolonged ventilation and increased risk of haemolysis. These patients have a very low threshold for haemolysis due to oxidative stress. Many commonly used drugs also predispose the individual for haemolysis when they are subjected to surgery. Here we present a known case of G6PD deficient patient with symptoms of breathlessness for the last nine years who was taken for surgery with pre-planned precautionary measures to avoid unnecessary haemolysis...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28208242/chemiluminescence-of-lucigenin-allantoin-and-its-application-for-the-detection-of-allantoin
#20
Muhammad Saqib, Baohua Lou, Mohamed Ibrahim Halawa, Shimeles Addisu Kitte, Zhongyuan Liu, Guobao Xu
Allantoin has been reported as a promising biomarker for monitoring of oxidative stress in humans and widely utilized in a variety of topical pharmaceuticals and cosmetics. Currently, the detection of allantoin is achieved by using chromatographic coupled techniques, which needs sample pre-extraction, derivatization, complex matrixes, and expensive instrumentation. Herein we report both the intense chemiluminescence of allantoin with lucigenin and the chemiluminescent detection of allantoin for the first time...
February 7, 2017: Analytical Chemistry
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