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NaHS and vasodilation

Mei Wang, Youyang Hu, Yifei Fan, Yan Guo, Fanglin Chen, Shuo Chen, Qinglin Li, Zhiwu Chen
BACKGROUND/AIM: H2S is a novel vasoactivator. To verify the hypothesis that H2S may act as an endothelium-derived hyperpolarizing factor (EDHF) in the rat cerebrovasculature, the role of H2S in endothelium-derived relaxing factor (EDRF)-mediated responses was investigated. METHODS: Cystathionine-γ-lyase (CSE) was knocked down with an siRNA technique. Artery diameter, hyperpolarization and Ca2+-activated K+ (KCa) current were measured. RESULTS: CSE knockdown was indicated by a decrease in protein and mRNA expression in the rat middle cerebral artery (MCA) and cerebral basilar artery (CBA)...
October 13, 2016: Journal of Vascular Research
Marcin Magierowski, Katarzyna Magierowska, Jakub Szmyd, Marcin Surmiak, Zbigniew Sliwowski, Slawomir Kwiecien, Tomasz Brzozowski
BACKGROUND: Alendronate is an inhibitor of osteoclast-mediated bone resorption, but its clinical utility is limited due to gastrointestinal complications including bleeding erosions. AIMS: We studied whether potent vasodilators hydrogen sulfide (H2S) and carbon monoxide (CO) can protect against alendronate-induced gastric lesions in rats exposed to mild stress. METHODS: Three series (A, B, and C) of Wistar rats received alendronate (150-700 mg/kg i...
November 2016: Digestive Diseases and Sciences
Yetik-Anacak Gunay, Sevin Gulnur, Ozzayım Ozge, Dereli Mehmet Vehbi, Ahmed Asif
Reactive oxygen species (ROS) decreases bioavailability of nitric oxide (NO) and impairs NO-dependent relaxations. Like NO, hydrogen sulfide (H2S) is an antioxidant and vasodilator; however, the effect of ROS on H2S-induced relaxations is unknown. Here we investigated whether ROS altered the effect of H2S on vascular tone in mouse aorta and determined whether resveratrol (RVT) protects it via H2S. Pyrogallol induced ROS formation. It also decreased H2S formation and relaxation induced by l-cysteine and in mouse aorta...
August 15, 2016: Vascular Pharmacology
Elise R Hedegaard, Anja Gouliaev, Anna K Winther, Daniel D R Arcanjo, Mathilde Aalling, Nirthika S Renaltan, Mark E Wood, Matthew Whiteman, Nini Skovgaard, Ulf Simonsen
Endogenous hydrogen sulfide (H2S) is involved in the regulation of vascular tone. We hypothesized that the lowering of calcium and opening of potassium (K) channels as well as calcium-independent mechanisms are involved in H2S-induced relaxation in rat mesenteric small arteries. Amperometric recordings revealed that free [H2S] after addition to closed tubes of sodium hydrosulfide (NaHS), Na2S, and GYY4137 [P-(4-methoxyphenyl)-P-4-morpholinyl-phosphinodithioic acid] were, respectively, 14%, 17%, and 1% of added amount...
January 2016: Journal of Pharmacology and Experimental Therapeutics
Gunay Yetik-Anacak, Mehmet V Dereli, Gulnur Sevin, Ozge Ozzayım, Yasemin Erac, Asif Ahmed
INTRODUCTION: Resveratrol (RVT) found in red wine protects against erectile dysfunction and relaxes penile tissue (corpus cavernosum) via a nitric oxide (NO) independent pathway. However, the mechanism remains to be elucidated. Hydrogen sulfide (H2 S) is a potent vasodilator and neuromodulator generated in corpus cavernosum. AIMS: We investigated whether RVT caused the relaxation of mice corpus cavernosum (MCC) through H2 S. METHODS: H2 S formation is measured by methylene blue assay and vascular reactivity experiments have been performed by DMT strip myograph in CD1 MCC strips...
October 2015: Journal of Sexual Medicine
Olan Jackson-Weaver, Jessica M Osmond, Jay S Naik, Laura V Gonzalez Bosc, Benjimen R Walker, Nancy L Kanagy
Ca(+) sparks are vascular smooth muscle cell (VSMC) Ca(2+)-release events that are mediated by ryanodine receptors (RyR) and promote vasodilation by activating large-conductance Ca(2+)-activated potassium channels and inhibiting myogenic tone. We have previously reported that exposing rats to intermittent hypoxia (IH) to simulate sleep apnea augments myogenic tone in mesenteric arteries through loss of hydrogen sulfide (H2S)-induced dilation. Because we also observed that H2S can increase Ca(2+) spark activity, we hypothesized that loss of H2S after IH exposure reduces Ca(2+) spark activity and that blocking Ca(2+) spark generation reduces H2S-induced dilation...
December 1, 2015: American Journal of Physiology. Heart and Circulatory Physiology
Kwi Young Kang, Chan Hong Jeon, Sung Jae Choi, Bo Young Yoon, Chan-Bum Choi, Chang Hoon Lee, Chang-Hee Suh, Choong Won Lee, Chul Soo Cho, Eon Jeong Nam, Eun-Mi Koh, Ho-Youn Kim, Hyo Jin Choi, Hyoun-Ah Kim, Jae-Bum Jun, Jaejoon Lee, Jinseok Kim, Jong Dae Ji, Jun Ki Min, Ki Jo Kim, Kichul Shin, Min Wook So, Seong Ryul Kwon, Seong-Kyu Kim, Seong-Su Nah, Seung-Ki Kwok, Soo-Kon Lee, Sung Won Lee, Sung-Hwan Park, Won Park, Yong-Beom Park, Young Ho Lee, Shin-Seok Lee, Dae Hyun Yoo
OBJECTIVES: Pulmonary arterial hypertension (PAH) is a major cause of mortality in connective tissue disease (CTD). The survival rates and mortality-predictive factors of a nationwide registry of Korean patients with CTD-PH measured by echocardiography were determined. METHODS: Patients with CTD-PH were enrolled between April 2008 and December 2012. Hemodynamic parameters and clinical data (WHO-functional class [FC], organ involvement, laboratory tests and treatment agents) were recorded...
July 27, 2015: International Journal of Rheumatic Diseases
Jun Han, Jia-li Xuan, Hao-ran Hu, Zhi-wu Chen
To investigate the effects and potential mechanisms of hyperoside (Hyp) on the vascular endothelium function in middle cerebral artery (MCA) ex vivo in rats. Isolated arterial segments from MCAs of rats were used for surveying vasomotoricity in a pressurized chamber. Transmembrane potential was recorded by using glass microelectrodes to evaluate hyperpolarization. Hyp (1 x 10(-6)-1 x 10(-4) mol . L-1) was utilized to observe the effect on 1 x 10(-7) mol . L-1 U46619-preconstricted MCA in rats. The results showed that 1 x 10(-6)-1 x 10(-4) mol ...
December 2014: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
H Xue, S Zhou, L Xiao, Q Guo, S Liu, Y Wu
As a novel gasotransmitter, hydrogen sulfide (H(2)S) has vasodilating and antihypertensive effects in cardiovascular system. Thus, we hypothesized that H(2)S might have beneficial effects on thoracic endothelial function in two-kidney one-clip (2K1C) rats, a model of renovascular hypertension. Sodium hydrosulfide (NaHS, 56 micromol/kg/day) was administrated intra-peritoneally from the third day after the 2K1C operation. Along with the development of hypertension, the systolic blood pressure (SBP) was measured before the operation and each week thereafter...
2015: Physiological Research
S Takır, G Z Ortaköylü, A Toprak, B S Uydeş-Doğan
Hydrogen sulphide (H2S) is known to be produced endogenously in ocular tissues with the highest levels in the retina and cornea. However, it is yet unclear whether it can modulate retinal arterial tone. Herein, we aimed to investigate the effectiveness and the mechanism of the action of H2S in the isolated bovine retinal arteries. For this purpose, the probable vasorelaxant and inhibitory effects of H2S on vascular reactivity were tested comparatively in the retinal arteries by using the donor, sodium hydrosulphide (NaHS)...
March 2015: Experimental Eye Research
Zaid Altaany, YoungJun Ju, Guangdong Yang, Rui Wang
The gasotransmitter hydrogen sulfide (H(2)S), which is generated by cystathionine γ-lyase (CSE), signals by modifying proteins through S-sulfhydration and potentially other mechanisms. A target protein for H(2)S is endothelial nitric oxide synthase (eNOS), an enzyme that generates nitric oxide (NO), which causes vasodilation. We investigated whether H(2)S-induced S-sulfhydration affected the S-nitrosylation and phosphorylation of eNOS and the functional effects of changes in these posttranslational modifications on eNOS activity...
September 9, 2014: Science Signaling
Pitchai Balakumar, Ying Hui Nyo, Raja Renushia, Devarajan Raaginey, Ann Nah Oh, Rajavel Varatharajan, Sokkalingam A Dhanaraj
Dipyridamole is a platelet inhibitor indicated for the secondary prevention of transient ischemic attack. It inhibits the enzyme phosphodiesterase, elevates cAMP and cGMP levels and prevents platelet aggregation. Dipyridamole inhibits the cellular uptake of adenosine into red blood cells, platelets and endothelial cells that results in increased extracellular availability of adenosine, leading to modulation of cardiovascular function. The antiplatelet action of dipyridamole might offer therapeutic benefits in secondary stroke prevention in combination with aspirin...
September 2014: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Fiaz Ud Din Ahmad, Munavvar A Sattar, Hassaan A Rathore, Yong Chia Tan, Safia Akhtar, Oh Hui Jin, Yen Pei Pei, Nor A Abdullah, Edward J Johns
Oxidative stress and suppressed H2S production lead to increased renal vascular resistance, disturbed glomerular hemodynamics, and abnormal renal sodium and water handling, contribute to the pathogenesis and maintenance of essential hypertension in man and the spontaneously hypertensive rat. This study investigated the impact of H2S and tempol alone and in combination on blood pressure and renal hemodynamics and excretory functions in the SHR. Groups of WKY rats or SHR (n=6) were treated for 4 weeks either as controls or received NaHS (SHR+NaHS), tempol (SHR+Tempol), or NaHS plus tempol (SHR+NaHS +Tempol)...
May 2014: Renal Failure
Kim M Holwerda, Suzanne D Burke, Marijke M Faas, Zsuzsanna Zsengeller, Isaac E Stillman, Peter M Kang, Harry van Goor, Amy McCurley, Iris Z Jaffe, S Ananth Karumanchi, A Titia Lely
Soluble fms-like tyrosine kinase 1 (sFlt1), a circulating antiangiogenic protein, is elevated in kidney diseases and contributes to the development of preeclampsia. Hydrogen sulfide is a vasorelaxant and proangiogenic gas with therapeutic potential in several diseases. Therefore, we evaluated the potential therapeutic effect and mechanisms of action of hydrogen sulfide in an animal model of sFlt1-induced hypertension, proteinuria, and glomerular endotheliosis created by adenovirus-mediated overexpression of sFlt1 in Sprague-Dawley rats...
April 2014: Journal of the American Society of Nephrology: JASN
Hua Cao, Xiaohong Zhou, Jianping Zhang, Xinli Huang, Yu Zhai, Xuejing Zhang, Li Chu
Hydrogen sulfide (H2S) displays vasodilative, anti-oxidative, anti-inflammatory and cytoprotective activities. The objective of this study was to evaluate the inhibitory effect of H2S on bleomycin (BLM)-induced pulmonary fibrosis in rats and its possible mechanisms. Fifty-four pathogen-free Male Wistar rats were randomly divided into three groups: control, BLM and H2S treated groups with 18 rats in each group. Each group was then divided into three subgroups based on time of study (7, 14 and 28 day). Pulmonary fibrosis model was established by a single intratracheal instillation of BLM A5 (5 mg/kg)...
January 30, 2014: Toxicology Letters
Mohammad R Al-Magableh, Barbara K Kemp-Harper, Hooi H Ng, Alyson A Miller, Joanne L Hart
The aim of this study was to examine the ability of H2S, released from NaHS to protect vascular endothelial function under conditions of acute oxidative stress by scavenging superoxide anions (O2(-)) and suppressing vascular superoxide anion production. O2(-) was generated in Krebs' solution by reacting hypoxanthine with xanthine oxidase (Hx-XO) or with the O2(-) generator pyrogallol to model acute oxidative stress in vitro. O2(-) generation was measured by lucigenin-enhanced chemiluminescence. Functional responses in mouse aortic rings were assessed using a small vessel myograph...
January 2014: Naunyn-Schmiedeberg's Archives of Pharmacology
Shilpi Agarwal, Meenu Gupta, Bijan Choudhury
In this work, acyltransferase activity of a new bacterial isolate Bacillus smithii strain IITR6b2 was utilized for the synthesis of nicotinic acid hydroxamate (NAH), a heterocyclic class of hydroxamic acid. NAH is an important pyridine derivative and has found its role as bioligand, urease inhibitor, antityrosinase, antioxidant, antimetastatic, and vasodilating agents. Amidase having acyltransferase activity with nicotinamide is suitable for nicotinic acid hydroxamate production. However, amidase can also simultaneously hydrolyze nicotinamide and nicotinic acid hydroxamate to nicotinic acid...
September 2013: Journal of Industrial Microbiology & Biotechnology
Olan Jackson-Weaver, Jessica M Osmond, Melissa A Riddle, Jay S Naik, Laura V Gonzalez Bosc, Benjimen R Walker, Nancy L Kanagy
We have previously shown that hydrogen sulfide (H₂S) reduces myogenic tone and causes relaxation of phenylephrine (PE)-constricted mesenteric arteries. This effect of H₂S to cause vasodilation and vascular smooth muscle cell (VSMC) hyperpolarization was mediated by large-conductance Ca(2+)-activated potassium channels (BKCa). Ca(2+) sparks are ryanodine receptor (RyR)-mediated Ca(2+)-release events that activate BKCa channels in VSMCs to cause membrane hyperpolarization and vasodilation. We hypothesized that H₂S activates Ca(2+) sparks in small mesenteric arteries...
June 1, 2013: American Journal of Physiology. Heart and Circulatory Physiology
Marcin Magierowski, Katarzyna Jasnos, Sławomir Kwiecień, Tomasz Brzozowski
Hydrogen sulfide (H2S) is commonly known as a toxic gas with an unpleasant odor. However, in the human body it plays a role as a gaseous transmitter involved in the control of physiological processes. Studies published so far have shown that H2S increased synaptic long-term potentiation in the central nervous system and exerted the inflammatory and anti-inflammatory effects on vascular endothelium. These effects clearly depend on the concentration of this gaseous molecule. H2S exerts vasodilatory effect in the cardiovascular system similar to those exhibited by carbon monoxide or nitric oxide...
2013: Postȩpy Higieny i Medycyny Doświadczalnej
A Martelli, L Testai, M C Breschi, K Lawson, N G McKay, F Miceli, M Taglialatela, V Calderone
Hydrogen sulphide (H2S) has been recently hypothesized to be an endogenous adipocyte-derived relaxing factor, evoking vasorelaxation of conductance and resistance vessels. Although the activation of ATP-sensitive potassium channels is known to play a central role in H2S-induced vasorelaxation, activation of vascular Kv7 voltage-gated potassium channels has also been suggested. To investigate this possibility, the ability of selective activators and blockers of distinct classes of potassium channels to affect vasodilation induced by the H2S-donor NaHS, as well as NaHS-induced Rb(+) efflux in endothelium-denuded rat aortic rings, was investigated...
April 2013: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
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