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electromagnetic cell differentiation

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https://www.readbyqxmd.com/read/28144594/effects-of-rf-emf-exposure-from-gsm-mobile-phones-on-proliferation-rate-of-human-adipose-derived-stem-cells-an-in-vitro-study
#1
D Shahbazi-Gahrouei, B Hashemi-Beni, Z Ahmadi
BACKGROUND: As the use of mobile phones is increasing, public concern about the harmful effects of radiation emitted by these devices is also growing. In addition, protection questions and biological effects are among growing concerns which have remained largely unanswered. Stem cells are useful models to assess the effects of radiofrequency electromagnetic fields (RF-EMF) on other cell lines. Stem cells are undifferentiated biological cells that can differentiate into specialized cells...
December 2016: Journal of Biomedical Physics & Engineering
https://www.readbyqxmd.com/read/28002911/extracorporeal-shock-waves-induce-osteogenic-differentiation-of-human-bone-marrow-stromal-cells
#2
A Notarnicola, G Vicenti, G Maccagnano, F Silvestis, P Cafforio, B Moretti
The effects of treatment with shock waves (SW) on osteoblastic cells have already been described. Furthermore, the effects of treatment with SW are also determined by the contextual stimulation of other cell lines, in particular of mesenchymal cells. This is the first experimental study of stimulation of a human mesenchymal stem cell line, taken from bone marrow, using SW (electromagnetic device), with two energy levels. The results showed a significant increase in expression of the main osteoblastic differentiation genes: BMP2, alkaline phosphatase, osteocalcin, COL1A1, RUNX2...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/27986082/mesenchymal-stem-cells-as-therapeutic-target-of-biophysical-stimulation-for-the-treatment-of-musculoskeletal-disorders
#3
REVIEW
Marco Viganò, Valerio Sansone, Maria Cristina d'Agostino, Pietro Romeo, Carlotta Perucca Orfei, Laura de Girolamo
BACKGROUND: Musculoskeletal disorders are regarded as a major cause of worldwide morbidity and disability, and they result in huge costs for national health care systems. Traditional therapies frequently turned out to be poorly effective in treating bone, cartilage, and tendon disorders or joint degeneration. As a consequence, the development of novel biological therapies that can treat more effectively these conditions should be the highest priority in regenerative medicine. Mesenchymal stem cells (MSCs) represent one of the most promising tools in musculoskeletal tissue regenerative medicine, thanks to their proliferation and differentiation potential and their immunomodulatory and trophic ability...
December 16, 2016: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/27901473/influence-of-solar-and-geomagnetic-activity-in-gymnodinium-catenatum-dinophyceae-cultures
#4
Paulo Vale
Laboratory cultures of the paralytic shellfish poisoning producing microalga Gymnodinium catenatum were subjected to a hypo-osmotic shock and changes in cell concentration were observed in two separate experiments of 8 and 24 hours duration, respectively. The increase in geomagnetic activity (GMA), radio and X-ray fluxes and solar X-ray flares were negatively correlated with cell numbers. Cell losses were observed in the short experiment, but not in the longest one. GMA action was related to the course of the experimental period, while electromagnetic radiation (EMR) was only significantly related when the previous hours before the experiments were considered...
January 2017: General Physiology and Biophysics
https://www.readbyqxmd.com/read/27881341/-low-frequency-pulsed-electromagnetic-fields-promotes-rat-osteoblast-differentiation-in-vitro-through-camp-pka-signal-pathway
#5
Qing-Qing Fang, Zhi-Zhong Li, Jian Zhou, Wen-Gui Shi, Juan-Li Yan, Yan-Fang Xie, Ke-Ming Chen
OBJECTIVE: To study whether low-frequency pulsed electromagnetic fields promotes the differentiation of cultured rat osteoblasts through the cAMP/PKA signal pathway. METHODS: Rat calvarial osteoblasts isolated by enzyme digestion were exposed to 50 Hz 0.6 mT low-frequency pulsed electromagnetic field for varying lengths of time, and the concentration of cAMP and levels of phosphorylated PKA in the cells were assayed. In cells treated with DDA to inhibit the activity of adenylate cyclase, the changes of ALP activity and transcription of osteogenic gene were detected after exposure to low-frequency pulsed electromagnetic field...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27859405/effects-of-bmp9-and-pulsed-electromagnetic-fields-on-the-proliferation-and-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells
#6
Tingting Wang, Pei Wang, Zhizhong Cao, Xingxing Wang, Dalin Wang, Yaxian Shen, Da Jing, Erping Luo, Weizhong Tang
Periodontal ligament stem cells (PDLSCs) have been confirmed to have self-renewal capacity and multidifferentiation potential and are good candidates for periodontal tissue regeneration. Pulsed electromagnetic field (PEMF) has been demonstrated to promote osteogenesis in non-union fractures, partly by regulating mesenchymal stem cells or osteoblast activity. However, there is no report about the osteo-inductive effect of PEMF stimulation on human PDLSCs (hPDLSCs). Thus, we tested the hypothesis that PEMF biophysical stimulation alone has an influence on the proliferation and osteogenic differentiation of hPDLSCs...
January 2017: Bioelectromagnetics
https://www.readbyqxmd.com/read/27857683/in-vitro-magnetic-stimulation-a-simple-stimulation-device-to-deliver-defined-low-intensity-electromagnetic-fields
#7
Stephanie Grehl, David Martina, Catherine Goyenvalle, Zhi-De Deng, Jennifer Rodger, Rachel M Sherrard
Non-invasive brain stimulation (NIBS) by electromagnetic fields appears to benefit human neurological and psychiatric conditions, although the optimal stimulation parameters and underlying mechanisms remain unclear. Although, in vitro studies have begun to elucidate cellular mechanisms, stimulation is delivered by a range of coils (from commercially available human stimulation coils to laboratory-built circuits) so that the electromagnetic fields induced within the tissue to produce the reported effects are ill-defined...
2016: Frontiers in Neural Circuits
https://www.readbyqxmd.com/read/27856256/pulsed-electromagnetic-field-inhibits-rankl-dependent-osteoclastic-differentiation-in-raw264-7-cells-through-the-ca-2-calcineurin-nfatc1-signaling-pathway
#8
Jie Zhang, Haixia Xu, Zhongyu Han, Ping Chen, Qiang Yu, Yutian Lei, Zongze Li, Ming Zhao, Jing Tian
Pulsed electromagnetic field (PEMF) has been reported to improve bone healing in osteoporosis patients. However, the precise mechanism has remained largely unknown. This study aimed to investigate the effects of PEMF on nuclear factor κB ligand (RANKL)-dependent osteoclastic differentiation and the Ca(2+)-calcineurin-NFATc1 signaling pathway in RAW264.7 cells in vitro. Treating RAW264.7 cells with RANKL for 4 days induced osteoclastic differentiation in vitro, and the formation of multinucleated osteoclasts, bone resorption-pit formation, tartrate-resistant acid phosphatase (TRAP) activity and the protein levels of cathepsin K, TRAP, Nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) and matrix metalloproteinase 9 (MMP-9) were significantly decreased...
January 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27797153/the-use-of-electric-magnetic-and-electromagnetic-field-for-directed-cell-migration-and-adhesion-in-regenerative-medicine
#9
REVIEW
Christina L Ross
Directed cell migration and adhesion is essential to embryonic development, tissue formation and wound healing. For decades it has been reported that electric field (EF), magnetic field (MF) and electromagnetic field (EMF) can play important roles in determining cell differentiation, migration, adhesion, and evenwound healing. Combinations of these techniques have revealed new and exciting explanations for how cells move and adhere to surfaces; how the migration of multiple cells are coordinated and regulated; how cellsinteract with neighboring cells, and also to changes in their microenvironment...
October 31, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27790871/combined-effect-of-pulsed-electromagnetic-field-and-sound-wave-on-in-vitro-and-in-vivo-neural-differentiation-of-human-mesenchymal-stem-cells
#10
Yun-Kyong Choi, Enerelt Urnukhsaikhan, Hee-Hoon Yoon, Young-Kwon Seo, Hyunjin Cho, Jong-Seob Jeong, Soo-Chan Kim, Jung-Keug Park
Biophysical wave stimulus has been used as an effective tool to promote cellular maturation and differentiation in the construction of engineered tissue. Pulsed electromagnetic fields (PEMFs) and sound waves have been selected as effective stimuli that can promote neural differentiation. The aim of this study was to investigate the synergistic effect of PEMFs and sound waves on the neural differentiation potential in vitro and in vivo using human bone marrow mesenchymal stem cells (hBM-MSCs). In vitro, neural-related genes in hBM-MSCs were accelerated by the combined exposure to both waves more than by individual exposure to PEMFs or sound waves...
October 28, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27635198/mesenchymal-stem-cells-that-located-in-the-electromagnetic-fields-improves-rat-model-of-parkinson-s-disease
#11
Majid Jadidi, Saeed Moghadas Biat, Hamid Reza Sameni, Manouchehr Safari, Abbas Ali Vafaei, Laya Ghahari
OBJECTIVES: The main characteristic of mesenchymal stem cells (MSCs) is their ability to produce other cell types. Electromagnetic field (EMF) stimulates differentiation of MSCs into other cells. In this study, we investigated whether EMF can effect on the differentiation of MSCs into dopaminergic (DA) neurons. MATERIALS AND METHODS: An EMF with a frequency of 50 Hz and two intensities of 40 and 400 µT 1hr/day was generated around the cells for a week. Afterwards, these cells were injected into the left ventricle of Parkinsonian rats...
July 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27630630/exposure-of-elf-emf-and-rf-emf-increase-the-rate-of-glucose-transport-and-tca-cycle-in-budding-yeast
#12
Kang-Wei Lin, Chuan-Jun Yang, Hui-Yong Lian, Peng Cai
In this study, we investigated the transcriptional response to 50 Hz extremely low frequency electromagnetic field (ELF-EMF) and 2.0 GHz radio frequency electromagnetic field (RF-EMF) exposure by Illumina sequencing technology using budding yeast as the model organism. The transcription levels of 28 genes were upregulated and those of four genes were downregulated under ELF-EMF exposure, while the transcription levels of 29 genes were upregulated and those of 24 genes were downregulated under RF-EMF exposure...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27599240/cell-therapy-for-spinal-cord-injury-informed-by-electromagnetic-waves
#13
Jack Finnegan, Hui Ye
Spinal cord injury devastates the CNS, besetting patients with symptoms including but not limited to: paralysis, autonomic nervous dysfunction, pain disorders and depression. Despite the identification of several molecular and genetic factors, a reliable regenerative therapy has yet to be produced for this terminal disease. Perhaps the missing piece of this puzzle will be discovered within endogenous electrotactic cellular behaviors. Neurons and stem cells both show mediated responses (growth rate, migration, differentiation) to electromagnetic waves, including direct current electric fields...
October 2016: Regenerative Medicine
https://www.readbyqxmd.com/read/27527723/effects-of-exposure-to-extremely-low-frequency-electromagnetic-fields-on-the-differentiation-of-th17-t-cells-and-regulatory-t-cells
#14
Yun-Jung Lee, Kyeong Eun Hyung, Jong-Sun Yoo, Ye Won Jang, Soo Jeong Kim, Do Ik Lee, Sang Joon Lee, So-Young Park, Ji Hoon Jeong, Kwang Woo Hwang
The potential risks that electromagnetic fields (EMF) pose to human physiology have been debated for several decades, especially considering that EMF is almost omnipresent and some occupations involve regular exposure to particularly strong fields. In the present study, the effects of 60 Hz 0.3 mT EMF on CD4+ T cells were evaluated. Production of T cell related cytokines, IFN-γ and IL-2, was not altered in CD4+ T cells that were exposed to EMF, and cell proliferation was also unaffected. The expression of genes present in a subset of Th17 cells was upregulated following EMF exposure, and the production of effector cytokines of the IL-17A subset also increased...
October 2016: General Physiology and Biophysics
https://www.readbyqxmd.com/read/27520819/molecular-mechanisms-contributing-to-the-growth-and-physiology-of-an-extremophile-cultured-with-dielectric-heating
#15
Kathleen D Cusick, Baochuan Lin, Anthony P Malanoski, Sarah M Strycharz-Glaven, Allison Cockrell-Zugell, Lisa A Fitzgerald, Jeffrey A Cramer, Daniel E Barlow, Thomas J Boyd, Justin C Biffinger
: The effect of microwave frequency electromagnetic fields on living microorganisms is an active and highly contested area of research. One of the major drawbacks to using mesophilic organisms to study microwave radiation effects is the unavoidable heating of the organism, which has limited the scale (<5 ml) and duration (<1 h) of experiments. However, the negative effects of heating a mesophile can be mitigated by employing thermophiles (organisms able to grow at temperatures of >60°C)...
October 15, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27515859/effect-of-extremely-low-frequency-electromagnetic-field-on-map2-and-nestin-gene-expression-of-hair-follicle-dermal-papilla-cells
#16
Marzie Moraveji, Nooshin Haghighipour, Hamid Keshvari, Tannaz Nourizadeh Abbariki, Mohammad Ali Shokrgozar, Amir Amanzadeh
INTRODUCTION: In recent years, the extremely low frequency electromagnetic field (ELF-EMF) has attracted a great deal of scientific interest. The ELF-EMF signal is able to control ion transport across ion channels and therefore induce cell differentiation. AIM: The purpose of this study was to investigate the effect of ELF-EMF (50 Hz, 1 mT) on MAP2 and Nestin gene expression of dermal papilla mesenchymal cells (DPCs). METHODS: In order to examine the effect of chemical and electromagnetic factors on gene expression, 4 experimental groups, namely chemical (cell exposure to chemical signals), EMF (exposing cells to ELF-EMF), chemical-EMF (subjecting cells to chemical signals and ELF-EMF) and control (with no treatment) groups, were prepared, treated for 5 days, and studied...
August 19, 2016: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/27398987/the-regenerative-effects-of-electromagnetic-field-on-spinal-cord-injury
#17
REVIEW
Christina L Ross, Ishaq Syed, Thomas L Smith, Benjamin S Harrison
Traumatic spinal cord injury (SCI) is typically the result of direct mechanical impact to the spine, leading to fracture and/or dislocation of the vertebrae along with damage to the surrounding soft tissues. Injury to the spinal cord results in disruption of axonal transmission of signals. This primary trauma causes secondary injuries that produce immunological responses such as neuroinflammation, which perpetuates neurodegeneration and cytotoxicity within the injured spinal cord. To date there is no FDA-approved pharmacological agent to prevent the development of secondary SCI and induce regenerative processes aimed at healing the spinal cord and restoring neurological function...
2017: Electromagnetic Biology and Medicine
https://www.readbyqxmd.com/read/27356174/effects-of-electromagnetic-fields-on-osteoporosis-a-systematic-literature-review
#18
REVIEW
Rong Wang, Hua Wu, Yong Yang, Mingyu Song
Electromagnetic fields (EMFs) as a safe, effective and noninvasive treatment have been researched and used for many years in orthopedics, and the common use clinically is to promote fracture healing. The effects of EMFs on osteoporosis have not been well concerned. The balance between osteoblast and osteoclast activity as well as the balance between osteogenic differentiation and adipogenic differentiation of bone marrow mesenchymal stem cells plays an important role in the process of osteoporosis. A number of recent reports suggest that EMFs have a positive impact on the balances...
2016: Electromagnetic Biology and Medicine
https://www.readbyqxmd.com/read/27355896/choice-of-osteoblast-model-critical-for-studying-the-effects-of-electromagnetic-stimulation-on-osteogenesis-in-vitro
#19
Anna-Maria Bique, Emilia Kaivosoja, Marko Mikkonen, Mervi Paulasto-Kröckel
The clinical benefits of electromagnetic field (EMF) therapy in enhancing osteogenesis have been acknowledged for decades, but agreement regarding the underlying mechanisms continues to be sought. Studies have shown EMFs to promote osteoblast-like cell proliferation, or contrarily, to induce differentiation and enhance mineralization. Typically these disparities have been attributed to methodological differences. The present paper argues the possibility that the chosen osteoblast model impacts stimulation outcome...
2016: Electromagnetic Biology and Medicine
https://www.readbyqxmd.com/read/27332788/pulsed-electromagnetic-field-assisted-reduced-graphene-oxide-substrates-for-multidifferentiation-of-human-mesenchymal-stem-cells
#20
Ki-Taek Lim, Hoon Seonwoo, Kyung Soon Choi, Hexiu Jin, Kyung-Je Jang, Jangho Kim, Jin-Woo Kim, Soo Young Kim, Pill-Hoon Choung, Jong Hoon Chung
Electromagnetic fields (EMFs) can modulate cell proliferation, DNA replication, wound healing, cytokine expression, and the differentiation of mesenchymal stem cells (MSCs). Graphene, a 2D crystal of sp(2) -hybridized carbon atoms, has entered the spotlight in cell and tissue engineering research. However, a combination of graphene and EMFs has never been applied in tissue engineering. This study combines reduced graphene oxide (RGO) and pulsed EMFs (PEMFs) on the osteogenesis and neurogenesis of MSCs. First, the chemical properties of RGO are measured...
August 2016: Advanced Healthcare Materials
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