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electromagnetic cell differentiation

Majid Jadidi, Saeed Moghadas Biat, Hamid Reza Sameni, Manouchehr Safari, Abbas Ali Vafaei, Laya Ghahari
OBJECTIVES: The main characteristic of mesenchymal stem cells (MSCs) is their ability to produce other cell types. Electromagnetic field (EMF) stimulates differentiation of MSCs into other cells. In this study, we investigated whether EMF can effect on the differentiation of MSCs into dopaminergic (DA) neurons. MATERIALS AND METHODS: An EMF with a frequency of 50 Hz and two intensities of 40 and 400 µT 1hr/day was generated around the cells for a week. Afterwards, these cells were injected into the left ventricle of Parkinsonian rats...
July 2016: Iranian Journal of Basic Medical Sciences
Kang-Wei Lin, Chuan-Jun Yang, Hui-Yong Lian, Peng Cai
In this study, we investigated the transcriptional response to 50 Hz extremely low frequency electromagnetic field (ELF-EMF) and 2.0 GHz radio frequency electromagnetic field (RF-EMF) exposure by Illumina sequencing technology using budding yeast as the model organism. The transcription levels of 28 genes were upregulated and those of four genes were downregulated under ELF-EMF exposure, while the transcription levels of 29 genes were upregulated and those of 24 genes were downregulated under RF-EMF exposure...
2016: Frontiers in Microbiology
Jack Finnegan, Hui Ye
Spinal cord injury devastates the CNS, besetting patients with symptoms including but not limited to: paralysis, autonomic nervous dysfunction, pain disorders and depression. Despite the identification of several molecular and genetic factors, a reliable regenerative therapy has yet to be produced for this terminal disease. Perhaps the missing piece of this puzzle will be discovered within endogenous electrotactic cellular behaviors. Neurons and stem cells both show mediated responses (growth rate, migration, differentiation) to electromagnetic waves, including direct current electric fields...
October 2016: Regenerative Medicine
Yun-Jung Lee, Kyeong Eun Hyung, Jong-Sun Yoo, Ye Won Jang, Soo Jeong Kim, Do Ik Lee, Sang Joon Lee, So-Young Park, Ji Hoon Jeong, Kwang Woo Hwang
The potential risks that electromagnetic fields (EMF) pose to human physiology have been debated for several decades, especially considering that EMF is almost omnipresent and some occupations involve regular exposure to particularly strong fields. In the present study, the effects of 60 Hz 0.3 mT EMF on CD4+ T cells were evaluated. Production of T cell related cytokines, IFN-γ and IL-2, was not altered in CD4+ T cells that were exposed to EMF, and cell proliferation was also unaffected. The expression of genes present in a subset of Th17 cells was upregulated following EMF exposure, and the production of effector cytokines of the IL-17A subset also increased...
August 16, 2016: General Physiology and Biophysics
Kathleen D Cusick, Baochuan Lin, Anthony P Malanoski, Sarah M Strycharz-Glaven, Allison Cockrell-Zugell, Lisa A Fitzgerald, Jeffrey A Cramer, Daniel E Barlow, Thomas J Boyd, Justin C Biffinger
: The effect of microwave frequency electromagnetic fields on living microorganisms is an active and highly contested area of research. One of the major drawbacks to using mesophilic organisms to study microwave radiation effects is the unavoidable heating of the organism, which has limited the scale (<5 ml) and duration (<1 h) of experiments. However, the negative effects of heating a mesophile can be mitigated by employing thermophiles (organisms able to grow at temperatures of >60°C)...
October 15, 2016: Applied and Environmental Microbiology
Marzie Moraveji, Nooshin Haghighipour, Hamid Keshvari, Tannaz Nourizadeh Abbariki, Mohammad Ali Shokrgozar, Amir Amanzadeh
INTRODUCTION: In recent years, the extremely low frequency electromagnetic field (ELF-EMF) has attracted a great deal of scientific interest. The ELF-EMF signal is able to control ion transport across ion channels and therefore induce cell differentiation. AIM: The purpose of this study was to investigate the effect of ELF-EMF (50 Hz, 1 mT) on MAP2 and Nestin gene expression of dermal papilla mesenchymal cells (DPCs). METHODS: In order to examine the effect of chemical and electromagnetic factors on gene expression, 4 experimental groups, namely chemical (cell exposure to chemical signals), EMF (exposing cells to ELF-EMF), chemical-EMF (subjecting cells to chemical signals and ELF-EMF) and control (with no treatment) groups, were prepared, treated for 5 days, and studied...
August 19, 2016: International Journal of Artificial Organs
Christina L Ross, Ishaq Syed, Thomas L Smith, Benjamin S Harrison
Traumatic spinal cord injury (SCI) is typically the result of direct mechanical impact to the spine, leading to fracture and/or dislocation of the vertebrae along with damage to the surrounding soft tissues. Injury to the spinal cord results in disruption of axonal transmission of signals. This primary trauma causes secondary injuries that produce immunological responses such as neuroinflammation, which perpetuates neurodegeneration and cytotoxicity within the injured spinal cord. To date there is no FDA-approved pharmacological agent to prevent the development of secondary SCI and induce regenerative processes aimed at healing the spinal cord and restoring neurological function...
July 11, 2016: Electromagnetic Biology and Medicine
Rong Wang, Hua Wu, Yong Yang, Mingyu Song
Electromagnetic fields (EMFs) as a safe, effective and noninvasive treatment have been researched and used for many years in orthopedics, and the common use clinically is to promote fracture healing. The effects of EMFs on osteoporosis have not been well concerned. The balance between osteoblast and osteoclast activity as well as the balance between osteogenic differentiation and adipogenic differentiation of bone marrow mesenchymal stem cells plays an important role in the process of osteoporosis. A number of recent reports suggest that EMFs have a positive impact on the balances...
2016: Electromagnetic Biology and Medicine
Anna-Maria Bique, Emilia Kaivosoja, Marko Mikkonen, Mervi Paulasto-Kröckel
The clinical benefits of electromagnetic field (EMF) therapy in enhancing osteogenesis have been acknowledged for decades, but agreement regarding the underlying mechanisms continues to be sought. Studies have shown EMFs to promote osteoblast-like cell proliferation, or contrarily, to induce differentiation and enhance mineralization. Typically these disparities have been attributed to methodological differences. The present paper argues the possibility that the chosen osteoblast model impacts stimulation outcome...
2016: Electromagnetic Biology and Medicine
Ki-Taek Lim, Hoon Seonwoo, Kyung Soon Choi, Hexiu Jin, Kyung-Je Jang, Jangho Kim, Jin-Woo Kim, Soo Young Kim, Pill-Hoon Choung, Jong Hoon Chung
Electromagnetic fields (EMFs) can modulate cell proliferation, DNA replication, wound healing, cytokine expression, and the differentiation of mesenchymal stem cells (MSCs). Graphene, a 2D crystal of sp(2) -hybridized carbon atoms, has entered the spotlight in cell and tissue engineering research. However, a combination of graphene and EMFs has never been applied in tissue engineering. This study combines reduced graphene oxide (RGO) and pulsed EMFs (PEMFs) on the osteogenesis and neurogenesis of MSCs. First, the chemical properties of RGO are measured...
August 2016: Advanced Healthcare Materials
Bettina Hiemer, Josefin Ziebart, Anika Jonitz-Heincke, Philip Christian Grunert, Yukun Su, Doris Hansmann, Rainer Bader
The application of electromagnetic fields to support the bone-healing processes is a therapeutic approach for patients with musculoskeletal disorders. The ASNIS-III s-series screw is a bone stimulation system providing electromagnetic stimulation; however, its influence on human osteoblasts (hOBs) has not been extensively investigated. Therefore, in the present study, the impact of this system on the viability and differentiation of hOBs was examined. We used the ASNIS-III s screw system in terms of a specific experimental test set-up...
July 2016: International Journal of Molecular Medicine
Mengyao Liu, Carlin Lee, Dominique Laron, Nianli Zhang, Erik I Waldorff, James T Ryaby, Brian Feeley, Xuhui Liu
The post-surgery integrity of the tendons and muscle quality are the two major factors in success of rotator cuff (RC) repair. Though surgical techniques for rotator cuff repair have significantly improved in the past two decades, there are no effective treatments to improve tendon-to-bone healing and muscle quality after repair at this point in time. Pulsed electromagnetic fields (PEMF) have previously been used for promoting fracture healing. Previous studies have shown that PEMF has a positive role in promoting osteoblast precursors proliferation and differentiation...
May 2, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Aleksandra Maziarz, Beata Kocan, Mariusz Bester, Sylwia Budzik, Marian Cholewa, Takahiro Ochiya, Agnieszka Banas
The electromagnetic field (EMF) has a great impact on our body. It has been successfully used in physiotherapy for the treatment of bone disorders and osteoarthritis, as well as for cartilage regeneration or pain reduction. Recently, EMFs have also been applied in in vitro experiments on cell/stem cell cultures. Stem cells reside in almost all tissues within the human body, where they exhibit various potential. These cells are of great importance because they control homeostasis, regeneration, and healing. Nevertheless, stem cells when become cancer stem cells, may influence the pathological condition...
2016: Stem Cell Research & Therapy
Sibel Türedi, Hatice Hancı, Serdar Çolakoğlu, Haydar Kaya, Ersan Odacı
The effects on human health of electromagnetic field (EMF) have begun to be seriously questioned with the entry into daily life of devices establishing EMF, such as cell phones, wireless fidelity, and masts. Recent studies have reported that exposure to EMF, particularly during pregnancy, affects the developing embryo/fetus. The aim of this study was therefore to examine the effects of exposure to continuous 900-Megahertz (MHz) EMF applied in the prenatal period on ovarian follicle development and oocyte differentiation...
June 2016: International Journal of Radiation Biology
Wei-Hong Hei, Soochan Kim, Joo-Cheol Park, Young-Kwon Seo, Soung-Min Kim, Jeong Won Jahng, Jong-Ho Lee
The purpose of this study was to investigate the effect of Schwann-like cells combined with pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Schwann-like cells were derived from human dental pulp stem cells (hDPSCs) and verified with CD104, S100, glial fibrillary acidic protein (GFAP), laminin, and P75(NTR) immunocytochemistry. Gene expression of P75(NTR) and S100 were analyzed. Male Sprague-Dawley rats (200-250g, 6-week-old) were divided into seven groups (n = 10 each): control, sham, PEMF, hDPSCs, hDPSCs + PEMF, Schwann-like cells, Schwann-like cells + PEMF...
March 15, 2016: Bioelectromagnetics
Hee-Gyeong Yi, Kyung Shin Kang, Jung Min Hong, Jinah Jang, Moon Nyeo Park, Young Hun Jeong, Dong-Woo Cho
In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration. However, these studies were conducted using different frequencies of EMF to stimulate chondrocytes. Thus, it is necessary to investigate the effect of EMF frequency on cartilage formation. In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads...
July 2016: Journal of Biomedical Materials Research. Part A
Letizia Ferroni, Ilaria Tocco, Andrea De Pieri, Martina Menarin, Enrico Fermi, Adriano Piattelli, Chiara Gardin, Barbara Zavan
AIM: Pulsed electromagnetic field (PEMF) therapy has been documented to be an effective, non-invasive, safe treatment method for a variety of clinical conditions, especially in settings of recalcitrant healing. The underlying mechanisms on the different biological components of tissue regeneration are still to be elucidated. The aim of the present study was to characterize the effects of extremely low frequency (ELF)-PEMFs on commitment of mesenchymal stem cell (MSCs) culture system, through the determination of gene expression pattern and cellular morphology...
May 1, 2016: Life Sciences
Selvam Anbarasan, Ulaganathan Baraneedharan, Solomon Fd Paul, Harpreet Kaur, Subramoniam Rangaswami, Emmanuel Bhaskar
BACKGROUND: Pulsed electromagnetic field (PEMF) is used to treat bone and joint disorders for over 30 years. Recent studies demonstrate a significant effect of PEMF on bone and cartilage proliferation, differentiation, synthesis of extracellular matrix (ECM) and production of growth factors. The aim of this study is to assess if PEMF of low frequency, ultralow field strength and short time exposure have beneficial effects on in-vitro cultured human chondrocytes. MATERIALS AND METHODS: Primary human chondrocytes cultures were established using articular cartilage obtained from knee joint during joint replacement surgery...
January 2016: Indian Journal of Orthopaedics
Qinlong Ma, Chunhai Chen, Ping Deng, Gang Zhu, Min Lin, Lei Zhang, Shangcheng Xu, Mindi He, Yonghui Lu, Weixia Duan, Huifeng Pi, Zhengwang Cao, Liping Pei, Min Li, Chuan Liu, Yanwen Zhang, Min Zhong, Zhou Zhou, Zhengping Yu
Exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) can enhance hippocampal neurogenesis in adult mice. However, little is focused on the effects of ELF-EMFs on embryonic neurogenesis. Here, we studied the potential effects of ELF-EMFs on embryonic neural stem cells (eNSCs). We exposed eNSCs to ELF-EMF (50 Hz, 1 mT) for 1, 2, and 3 days with 4 hours per day. We found that eNSC proliferation and maintenance were significantly enhanced after ELF-EMF exposure in proliferation medium. ELF-EMF exposure increased the ratio of differentiated neurons and promoted the neurite outgrowth of eNSC-derived neurons without influencing astrocyes differentiation and the cell apoptosis...
2016: PloS One
Alexis J Haas, Yann Le Page, Maxim Zhadobov, Ronan Sauleau, Yves Le Dréan
Technologies for wireless telecommunication systems using millimeter waves (MMW) will be widely deployed in the near future. Forthcoming applications in this band, especially around 60GHz, are mainly developed for high data-rate local and body-centric telecommunications. At those frequencies, electromagnetic radiations have a very shallow penetration into biological tissues, making skin keratinocytes, and free nerve endings of the upper dermis the main targets of MMW. Only a few studies assessed the impact of MMW on neuronal cells, and none of them investigated a possible effect on neuronal differentiation...
April 8, 2016: Neuroscience Letters
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