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Mannose binding lectin

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https://www.readbyqxmd.com/read/28207759/investigations-on-therapeutic-glucocerebrosidases-through-paired-detection-with-fluorescent-activity-based-probes
#1
Wouter W Kallemeijn, Saskia Scheij, Sascha Hoogendoorn, Martin D Witte, Daniela Herrera Moro Chao, Cindy P A A van Roomen, Roelof Ottenhoff, Herman S Overkleeft, Rolf G Boot, Johannes M F G Aerts
Deficiency of glucocerebrosidase (GBA) causes Gaucher disease (GD). In the common non-neuronopathic GD type I variant, glucosylceramide accumulates primarily in the lysosomes of visceral macrophages. Supplementing storage cells with lacking enzyme is accomplished via chronic intravenous administration of recombinant GBA containing mannose-terminated N-linked glycans, mediating the selective uptake by macrophages expressing mannose-binding lectin(s). Two recombinant GBA preparations with distinct N-linked glycans are registered in Europe for treatment of type I GD: imiglucerase (Genzyme), contains predominantly Man(3) glycans, and velaglucerase (Shire PLC) Man(9) glycans...
2017: PloS One
https://www.readbyqxmd.com/read/28197138/genetic-and-phenotypic-screening-of-mannose-binding-lectin-in-relation-to-risk-of-recurrent-vulvovaginal-infections-in-women-of-north-india-a-prospective-cohort-study
#2
Namarta Kalia, Jatinder Singh, Sujata Sharma, Hardesh Arora, Manpreet Kaur
Recurrent Vulvovaginal Infections (RVVI) is common problem associated with women of reproductive age. The function and deleterious effect of Mannose Binding Lectin 2 (MBL2) common polymorphisms are reported to be associated with various diseases. However, the role of MBL2 promoter gene polymorphisms and their combined effect with structural variant along with Serum Mannose Binding Lectin (sMBL) levels in RVVI has not been investigated. The study included 258 RVVI cases and 203 age matched healthy controls. These were investigated for the distribution of MBL2 codon 54 and promoter polymorphisms by Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28157181/roles-of-n-glycans-in-the-polymerization-dependent-aggregation-of-mutant-ig-%C3%AE-chains-in-the-early-secretory-pathway
#3
Chiara Giannone, Claudio Fagioli, Caterina Valetti, Roberto Sitia, Tiziana Anelli
The polymeric structure of secretory IgM allows efficient antigen binding and complement fixation. The available structural models place the N-glycans bound to asparagines 402 and 563 of Ig-μ chains within a densely packed core of native IgM. These glycans are found in the high mannose state also in secreted IgM, suggesting that polymerization hinders them to Golgi processing enzymes. Their absence alters polymerization. Here we investigate their role following the fate of aggregation-prone mutant μ chains lacking the Cμ1 domain (μ∆)...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28150103/purification-biochemical-characterization-and-amino-acid-sequence-of-a-novel-type-of-lectin-from-aplysia-dactylomela-eggs-with-antibacterial-antibiofilm-potential
#4
Rômulo Farias Carneiro, Renato Cézar Farias Torres, Renata Pinheiro Chaves, Mayron Alves de Vasconcelos, Bruno Lopes de Sousa, André Castelo Rodrigues Goveia, Francisco Vassiliepe Arruda, Maria Nágila Carneiro Matos, Helena Matthews-Cascon, Valder Nogueira Freire, Edson Holanda Teixeira, Celso Shiniti Nagano, Alexandre Holanda Sampaio
A new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HCl-activated Sepharose™ media. Hemagglutination caused by ADEL was inhibited by several galactosides, mainly galacturonic acid (Ka = 6.05 × 10(6) M(-1)). The primary structure of ADEL consists of 217 residues, including 11 half-cystines involved in five intrachain and one interchain disulfide bond, resulting in a molecular mass of 57,228 ± 2 Da, as determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry...
February 1, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28144635/expression-analysis-of-lily-type-lectin-isotypes-in-the-rock-bream-oplegnathus-fasciatus-in-the-tissue-developmental-stage-and-viral-infection
#5
Young Mee Lee, In Jung Yang, Jae Koo Noh, Hyun Chul Kim, Choul-Ji Park, Jong-Won Park, Gyeong Eon Noh, Woo-Jin Kim, Kyung-Kil Kim
Lectins belong to the pattern-recognition receptors (PRRs) class and play important roles in the recognition and elimination of pathogens via the innate immune system. Recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks in the early immune response of fish. However, this study is limited to information that the lectin is involved in the innate immune response against viral infection. In the present study, the lily-type lectin-2 and -3 of Oplegnathus fasciatus (OfLTL-2 and 3) have been presented to be included B-lectin domain and two D-mannose binding sites in the amino acid sequence that an important feature for the fundamental structure...
December 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28132614/insight-into-the-intermolecular-recognition-mechanism-involved-in-complement-component-4-activation-through-serine-protease-trypsin
#6
Vikrant Kumar Sinha, Om Prakash Sharma, Muthuvel Suresh Kumar
Serine protease cleaved Complement component 4 (C4) at sessile loop, which is significant for completion of lectin and classical complement pathways at the time of infections. The co-crystalized structure of C4 with Mannose-binding protein-associated serine protease 2 (MASP2) provided the structural and functional aspects of its interaction and underlined the C4 activation by MASP2. The same study also revealed the significance of Complement control protein (CCP) domain through mutational study, where mutated CCP domain led to the inhibition of C4 activation...
January 29, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28130769/secretory-expression-and-characterization-of-chinese-narcissus-gna-like-lectin-in-pichia-pastoris
#7
Xinyu Li, Pengpeng Zhang, Ning Zhang, Xiao Liang, Wenya Wang, Qipeng Yuan, Qiang Li
Narcissus tazetta lectin (NTL) is a GNA-like lectin, which has a wide potential application in medicine, agriculture, and glycobiology. In the present paper, the codon-optimized ntl gene was transformed into the yeast Pichia pastoris; SDS-PAGE gel and western blotting analysis revealed that the recombinant lectin was expressed successfully in Pichia yeast. The similarity between the recombinant NTL and the native NTL was confirmed by circular dichroism (CD) and hemagglutination assay further. In the 5-L scale fermentator, the protein yield was as high as 1...
January 27, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28117986/sequence-controlled-glycopolymers-via-step-growth-polymerization-of-precision-glycomacromolecules-for-lectin-receptor-clustering
#8
Christoph Gerke, Morten F Ebbesen, Dennis Jansen, Sophia Boden, Tanja Freichel, Laura Hartmann
A versatile approach for the synthesis of sequence-controlled multiblock copolymers, using a combination of solid phase synthesis and step-growth polymerization by photoinduced thiol-ene coupling (TEC) is presented. Following this strategy, a series of sequence-controlled glycopolymers is derived from the polymerization of a hydrophilic spacer macromonomer and different glycomacromonomers bearing between one to five α-d-Mannose (Man) ligands. Through the solid phase assembly of the macromonomers, the number and positioning of spacer and sugar moieties is controlled and translates into the sequence-control of the final polymer...
February 8, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28111019/flexibility-in-mannan-binding-lectin-associated-serine-proteases-1-and-2-provides-insight-on-lectin-pathway-activation
#9
Ruodan Nan, Christopher M Furze, David W Wright, Jayesh Gor, Russell Wallis, Stephen J Perkins
The lectin pathway of complement is activated by complexes comprising a recognition component (mannose-binding lectin, serum ficolins, collectin-LK or collectin-K1) and a serine protease (MASP-1 or MASP-2). MASP-1 activates MASP-2, and MASP-2 cleaves C4 and C4b-bound C2. To clarify activation, new crystal structures of Ca(2+)-bound MASP dimers were determined, together with their solution structures from X-ray scattering, analytical ultracentrifugation, and atomistic modeling. Solution structures of the CUB1-EGF-CUB2 dimer of each MASP indicate that the two CUB2 domains were tilted by as much as 90° compared with the crystal structures, indicating considerable flexibility at the EGF-CUB2 junction...
February 7, 2017: Structure
https://www.readbyqxmd.com/read/28106805/synthetic-assembly-of-mannose-moieties-using-polymer-chemistry-and-the-biological-evaluation-of-its-interaction-towards-concanavalin-a
#10
Deepti Diwan, Kohei Shinkai, Toshihiro Tetsuka, Bin Cao, Hidenao Arai, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Protein-carbohydrate interactions exhibit myriad intracellular recognition events, so understanding and investigating their specific interaction with high selectivity and strength are of crucial importance. In order to examine the effect of multivalent binding on the specificity of protein-carbohydrate interactions, we synthesized mannose glycosides as a novel type of glycosylated monomer and glycopolymers of polyacrylamide derivatives with α-mannose (α-Man) by radical polymerization and monitored their strength of interaction with concanavalin A (Con A) by surface plasmon resonance (SPR) detection...
January 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28103877/rhodococcus-erythropolis-as-a-host-for-expression-secretion-and-glycosylation-of-mycobacterium-tuberculosis-proteins
#11
Antonio J Vallecillo, Cristina Parada, Pedro Morales, Clara Espitia
BACKGROUND: Glycosylation is one of the most abundant posttranslational polypeptide chain modification in nature. Although carbohydrate modification of protein antigens from many microbial pathogens constitutes important components of B cell epitopes, the role in T cell immunity is not completely understood. There is growing evidence about the importance of these modifications in host bacteria interactions in tuberculosis. It is known, that the sugars present in some Mycobacterium tuberculosis glycoproteins play an important role in both humoral and cellular immune response against the pathogen...
January 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28098871/lectin-pcl-inhibits-the-warburg-effect-of-pc3-cells-by-combining-with-egfr-and-inhibiting-hk2
#12
Hong Zhang, Xia Du, Ting-Ting Sun, Chun-Liu Wang, Ye Li, Shou-Zhen Wu
Prostatic carcinoma is the most aggressive tumor in adult men. Warburg effect is an important characteristic of tumor cell metabolism including prostate cancer cells, in which hexokinase 2 (HK2), a major rate-limiting enzyme involved in Warburg effect, is selectively upregulated. The lectin PCL is a mannose binding lectin which induces tumor cell apoptosis and autophagy. In the present study, we report that PCL could lower glucose consumption and lactate production, shift the Warburg effect by inhibiting the expression of HK2 in PC3 cells and the suppression of HK2 by siRNA reversed the effect of PCL on glucose consumption and lactate production...
January 16, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28097447/mannose-binding-lectin-mbl-codon-54-rs1800450-polymorphism-predisposes-towards-medium-vessel-vasculitis-in-patients-with-systemic-lupus-erythematosus
#13
Vir Singh Negi, Panneer Devaraju, Durga Prasanna Misra, Vikramraj K Jain, Jignesh Babulal Usdadiya, Paul T Antony, Reena Gulati
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease with multiple etiological factors. Mannose-binding lectin (MBL) plays a key role in innate immunity by activating antibody-independent lectin complement pathway, opsonisation, phagocytosis, and immune complex (IC) clearance. Genetic polymorphisms in the promoter and coding regions of MBL gene affect the circulatory levels and biological activity of MBL. Defects in MBL can lead to defective opsonisation and, hence, hamper clearance of apoptotic debris, the persistence of which can drive autoantibody formation in lupus...
January 17, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28086930/mannose-binding-lectin-associated-serine-protease-2-masp-2-contributes-to-poor-disease-outcome-in-humans-and-mice-with-pneumococcal-meningitis
#14
E Soemirien Kasanmoentalib, Mercedes Valls Seron, Bart Ferwerda, Michael W Tanck, Aeilko H Zwinderman, Frank Baas, Arie van der Ende, Matthijs C Brouwer, Diederik van de Beek
BACKGROUND: Pneumococcal meningitis is the most common and severe form of bacterial meningitis. Fatality rates are substantial, and long-term sequelae develop in about half of survivors. Disease outcome has been related to the severity of the pro-inflammatory response in the subarachnoid space. The complement system, which mediates key inflammatory processes, has been implicated as a modulator of pneumococcal meningitis disease severity in animal studies. METHODS: We investigated mannose-binding lectin-associated serine protease (MASP-2) levels in cerebrospinal fluid (CSF) samples derived from the diagnostic lumbar puncture, which was available for 307 of 792 pneumococcal meningitis episodes included in our prospective nationwide cohort study (39%), and the association between these levels and clinical outcome...
January 3, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28079948/induction-of-antibodies-directed-against-branched-core-o-mannosyl-glycopeptides-selectivity-complimentary-to-the-cona-lectin
#15
Jin Yu, Oliver C Grant, Christian Pett, Sabine Strahl, Robert J Woods, Ulrika Westerlind
Mammalian protein O-mannosylation comprise a group of post-translational modifications (PTMs) involved in muscle and brain development. Despite the enormous progress made, to date the biological impact of the mammalian O-mannosyl glycoproteome remains largely unknown. Tools are still needed to investigate the structure, role and abundance of O-mannosyl glycans. While O-mannosyl branching has been shown to be of relevance in integrin-dependent cell-migration, and also plays a role in demyelinating diseases, a broader understanding of the biological roles of branched O-mannosyl glycans is lacking in part due to the paucity of detection tools...
January 12, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28069433/dual-recognition-activity-of-a-rhamnose-binding-lectin-to-pathogenic-bacteria-and-zooxanthellae-in-stony-coral-pocillopora-damicornis
#16
Zhi Zhou, Xiaopeng Yu, Jia Tang, Yunjie Zhu, Guangmei Chen, Liping Guo, Bo Huang
Rhamnose-binding lectin (RBL) is a type of Ca(2+)-independent lectin with tandem repeat carbohydrate-recognition domain, and is crucial for the innate immunity in many invertebrates. In this study, the cDNA sequence encoding RBL in coral Pocillopora damicornis (PdRBL-1) was cloned. The PdRBL-1 protein shared highest amino acid sequence similarity (55%) with the polyp of Hydra vulgaris, and contained a signal peptide and two tandem carbohydrate-recognition domains in which all cysteine residues were conserved...
January 7, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28056798/high-rate-of-in-stent-restenosis-after-coronary-intervention-in-carriers-of-the-mutant-mannose-binding-lectin-allele
#17
Zsolt Bagyura, Loretta Kiss, Balázs Berta, Ágnes Szilágyi, Kristóf Hirschberg, Gábor Széplaki, Árpád Lux, Zsolt Szelid, Pál Soós, Béla Merkely
BACKGROUND: In-stent restenosis occurs in 10-30% of patients following bare metal stent (BMS) implantation and has various risk factors. Mannose-binding lectin (MBL) is known to have effect on the progression of atherosclerosis. Single nucleotide polymorphisms (SNP) of the MBL2 gene intron 1 (codon 52, 54, 57) are known to modulate the bioavailability of the MBL protein. Our aim was to identify the association of these polymorphisms of the MBL gene in the occurrence of in-stent restenosis after coronary artery bare metal stent implantation...
January 5, 2017: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/28052761/increased-activity-of-the-complement-system-in-cerebrospinal-fluid-of-the-patients-with-non-hiv-cryptococcal-meningitis
#18
Lei Shen, Jianming Zheng, Yan Wang, Mengqi Zhu, Haoxiang Zhu, Qi Cheng, Qian Li
BACKGROUNDS: Cryptococcal meningitis (CM) has been known to lead to significant morbidity and mortality. The relative contribution of the complement system in protection and pathogenesis during CM remains largely unknown. The purpose of this study was to evaluate the baseline complement component profiles in human cerebrospinal fluid (CSF) and plasma from non-HIV patients with CM, and therefore to provide insights of possible roles of the complement system in CM. METHODS: CSF and blood samples from forty two CM patients not infected with HIV and thirteen non-CM control patients (Ctrl) were retrospectively selected and evaluated from the patients admitted to the hospital with a suspected diagnosis of CM...
January 4, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28046067/c-type-lectin-receptor-dectin-2-binds-to-an-endogenous-protein-%C3%AE-glucuronidase-on-dendritic-cells
#19
Daiki Mori, Kensuke Shibata, Sho Yamasaki
C-type lectin receptors (CLRs) recognize pathogen-derived ligands and abnormal self that trigger protective immune responses. However, the precise nature of self ligands recognized by CLRs remains to be determined. Here, we found that Dectin-2 recognizes bone marrow-derived dendritic cells (BMDCs) using Dectin-2-expressing reporter cells. This activity was inhibited by an excessive amount of mannose, and by the mutation of mannose-binding motif in Dectin-2. β-glucuronidase (Gusb) was identified as a protein bound to Dectin-2 and mutations of N-glycosylation sites in Gusb impaired the binding of Gusb to Dectin-2...
2017: PloS One
https://www.readbyqxmd.com/read/28032346/mannose-binding-lectin-and-l-ficolin-polymorphisms-in-patients-with-community-acquired-pneumonia-caused-by-intracellular-pathogens
#20
Gijs van Kempen, Sabine Meijvis, Hendrik Endeman, Bart Vlaminckx, Bob Meek, Ben de Jong, Ger T Rijkers, Willem Jan Bos
Community acquired pneumonia (CAP) is the leading infectious disease requiring hospitalisation in the Western World. Genetic variability affecting the host response to infection may play a role in susceptibility and outcome in CAP patients. Mannose binding lectin (MBL) and L-ficolin (L-FCN) are two important activators of the complement system and they can enhance phagocytosis by opsonisation. In a prospective cohort of 505 Dutch CAP patients and 227 controls we studied whether polymorphisms in the MBL (MBL2) and FCN (FCN2) genes influenced susceptibility and outcome...
December 29, 2016: Immunology
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