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Mannose binding lectin

Laura Llull, Steffen Thiel, Sergio Amaro, Álvaro Cervera, Anna M Planas, Ángel Chamorro
Activation of the inflammatory generating complement system might play a pathogenic role in spontaneous subarachnoid hemorrhage (SAH). We studied whether plasma and cerebrospinal fluid (CSF) levels of complement proteins were associated with angiographic vasospasm and cerebral ischemic lesions after SAH. Ficolin-1 (M-ficolin), ficolin-3 (H-ficolin), mannose-binding lectin (MBL), MBL-associated serine protease 2 (MASP-2), MASP-3, and MAp44 were analyzed in plasma of 45 SAH patients at 24 h after bleeding. Additionally, ficolin-1 levels were measured in cerebrospinal fluid (CSF) samples obtained 24 h after bleeding in 19 patients with external ventricular drainage placement...
October 12, 2016: Molecular Neurobiology
Abirami Arasu, Venkatesh Kumaresan, Rajesh Palanisamy, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Munuswamy-Ramanujam Ganesh, Jesu Arockiaraj
Antimicrobial peptides (AMPs) are innate molecules that are found in a wide variety of species ranging from bacteria to humans. In recent years, excessive usage of antibiotics resulted in development of multi-drug resistant pathogens which made researchers to focus on AMPs as potential substitute for antibiotics. Lily type mannose-binding lectin is an extended super-family of structurally and evolutionarily related sugar binding proteins. These lectins are well-known AMPs which play important roles in fish defense mechanism...
October 8, 2016: Developmental and Comparative Immunology
Shigeyuki Kawa
High serum immunoglobulin (Ig) G4 concentration and abundant IgG4-bearing plasma cell infiltration are characteristic features in autoimmune pancreatitis (AIP). AIP is also complicated with a variety of other organ involvements that commonly share marked IgG4-bearing plasma cell infiltration, suggesting the existence of a systemic disease associated with IgG4 currently recognized as IgG4-related disease (IgG4-RD). However, it is controversial whether IgG4 plays a role in the pathogenesis of AIP or IgG4-RD through such characteristic attributes as Fab-arm exchange and rheumatoid factor (RF)-like activity...
September 21, 2016: Current Topics in Microbiology and Immunology
Xiang He, Lihua Li, Hong Xu, Jiang Xi, Xufeng Cao, Haoran Xu, Songhao Rong, Yilun Dong, Chuan Wang, Rongjun Chen, Jinghong Xu, Xiaoling Gao, Zhengjun Xu
Abiotic stresses have a significant impact on plant productivity and crop quality. Although plant lectins are thought to play important roles in plant defense signaling during pathogen attack, little is known about the contribution of plant lectins to stress resistance. We cloned and functionally characterized a rice jacalin-related mannose-binding lectin gene, OsJRL, from rice 'Nipponbare'. The cDNA of OsJRL contained a 438 bp open reading frame, which encodes a polypeptide of 145 amino acids. OsJRL was localized in the nucleus and cytoplasm...
October 8, 2016: Plant Biology
Toshi Horie, Megumi Inomata, Takeshi Into, Yoshiaki Hasegawa, Noriyuki Kitai, Fuminobu Yoshimura, Yukitaka Murakami
Bacterial glycoproteins are associated with physiological and pathogenic functions of bacteria. It remains unclear whether bacterial glycoproteins can bind to specific classes of lectins expressed on host cells. Tannerella forsythia is a gram-negative oral anaerobe that contributes to the development of periodontitis. In this study, we aimed to find lectin-binding glycoproteins in T. forsythia. We performed affinity chromatography of wheat germ agglutinin, which binds to N-acetylglucosamine (GlcNAc) and sialic acid (Sia), and identified OmpA-like protein as the glycoprotein that has the highest affinity...
2016: PloS One
Fei Chen, Cheng-Gang Ren, Tong Zhou, Yu-Jia Wei, Chuan-Chao Dai
Endophytes and plants can establish specific long-term symbiosis through the accumulation of secondary metabolites. Previous studies have shown that the endophytic fungus Gilmaniella sp. AL12 can stimulate Atractylodes lancea to produce volatile oils. The purpose of this report is to investigate key factors involved in the stimulation of A. lancea by AL12 and reveal the mechanism. We identified the active component from AL12 as an extracellular mannan with a polymerization degree of 26-42. Differential membrane proteomics of A...
October 5, 2016: Scientific Reports
Sabrina Lusvarghi, Katheryn Lohith, Jeanne Morin-Leisk, Rodolfo Ghirlando, Jenny E Hinshaw, Carole A Bewley
Carbohydrate binding proteins such as griffithsin, cyanovirin-N, and BanLec are potent HIV entry inhibitors and promising microbicides. Each binds to high-mannose glycans on the surface envelope glycoprotein gp120, yet the mechanisms by which they engage viral spikes and exhibit inhibition constants ranging from nanomolar to picomolar are not understood. To determine the structural and mechanistic basis for recognition and potency, we selected a panel of lectins possessing different valencies per subunit, oligomeric states, and relative orientations of carbohydrate binding sites to systematically probe their contributions to inhibiting viral entry...
October 3, 2016: ACS Infectious Diseases
Na Kong, Sheng Xie, Juan Zhou, Margarita Menéndez, Dolores Solís, JaeHyeung Park, Giampiero Proietti, Olof Ramström, Mingdi Yan
A new conjugation method for the immobilization of carbohydrates on nanomaterials was demonstrated simply by mixing perfluorophenyl azide-functionalized silica nanoparticles (SNPs), an amine-derivatized carbohydrate, and phenylacetaldehyde under ambient conditions without any catalyst. The density of carbohydrates on the glyconanoparticles was determined using the quantitative (19)F NMR ((19)F qNMR) technique; for example, the density of d-mannose (Man) on Man-SNPs was 2.5 ± 0.2 × 10(-16) nmol/nm(2). The glyconanoparticles retained their binding affinity and selectivity toward cognate lectins...
October 6, 2016: ACS Applied Materials & Interfaces
Diana Karpman, Ramesh Tati
Complement is activated during Shiga toxin-producing Escherichia coli-associated hemolytic uremic syndrome (STEC-HUS). There is evidence of complement activation via the alternative pathway in STEC-HUS patients as well as from in vivo and in vitro models. Ozaki et al. demonstrate activation of the mannose-binding lectin (MBL) pathway in Shiga toxin-treated mice expressing human MBL2, but lacking murine Mbls. Treatment with anti-human MBL2 antibody was protective, suggesting that MBL pathway activation also contributes to Shiga toxin-mediated renal injury...
October 2016: Kidney International
Laura Choteau, Francis Vasseur, Frederic Lepretre, Martin Figeac, Corine Gower-Rousseau, Laurent Dubuquoy, Daniel Poulain, Jean-Frederic Colombel, Boualem Sendid, Samir Jawhara
No abstract text is available yet for this article.
2016: Scientific Reports
A Liukkonen, Q He, U K Gürsoy, P J Pussinen, K Gröndahl-Yli-Hannuksela, J Liukkonen, T Sorsa, A L Suominen, S Huumonen, E Könönen
BACKGROUND AND OBJECTIVE: Mannose-binding lectin (MBL) plays an important role in innate immunity. MBL deficiency is usually caused by mutations in exon 1 of the MBL structural gene (MBL2). Our aim was to investigate MBL2 polymorphisms and their relation to salivary levels of periodontal inflammatory/tissue destruction markers and two major periodontitis-associated bacteria. MATERIAL AND METHODS: Salivary samples from 222 subjects were available for genotyping by pyrosequencing...
September 14, 2016: Journal of Periodontal Research
Hui-Lian Wang, Xi Lu, Xudong Yang, Nan Xu
PURPOSE: High-risk human papillomavirus (HR-HPV) infection is the main known cause of cervical cancer. Mannose-binding lectin (MBL) is a recognition molecule that mediates phagocytosis and activates complement. METHODS: We performed a meta-analysis to investigate the association of MBL-2 functional polymorphisms with HPV infection and cervical cancer (CC). RESULTS: The meta-analyses indicated an association between the MBL2 exon 1 polymorphisms and susceptibility to HPV infection in the recessive model (OO vs...
September 13, 2016: Archives of Gynecology and Obstetrics
Anna Sobiepanek, Małgorzata Milner-Krawczyk, Małgorzata Lekka, Tomasz Kobiela
Malignant melanoma is one of the most dangerous skin cancer originating from melanocytes. Thus, an early and proper melanoma diagnosis influences significantly the therapy efficiency. The melanoma recognition is still difficult, and generally, relies on subjective assessments. In particular, there is a lack of quantitative methods used in melanoma diagnosis and in the monitoring of tumour progression. One such method can be the atomic force microscopy (AFM) working in the force spectroscopy mode combined with quartz crystal microbalance (QCM), both applied to quantify the molecular interactions...
August 27, 2016: Biosensors & Bioelectronics
Donghao Xie, Xue-Qing Feng, Xi-Le Hu, Lin Liu, Zhihong Ye, Jun Cao, Guo-Rong Chen, Xiao-Peng He, Yi-Tao Long
This paper describes the development of a "diffusion-to-surface" ratiometric graphene electrosensor for the selective detection of live cells and pathogens that highly express mannose-binding proteins (MBPs). MBPs have been implicated in many pathological processes and are identified on specific types of bacteria. Consequently, MBPs are a promising biomarker for targeted disease diagnosis and therapy. Here, we develop a unique electrosensor that features a ratiometric voltammetric signal for the selective probing of MBPs...
September 28, 2016: ACS Applied Materials & Interfaces
Andrea Gelemanović, Katie Dobberpuhl, Goran Krakar, Inga Patarčić, Ivana Kolčić, Ozren Polašek
AIM: To summarize available evidence on the role of host genetics in the susceptibility to congenital and childhood cytomegalovirus (CMV) infections by conducting a systematic review of published studies. METHODS: We searched online databases (PubMed, Web of Science, Scopus and HuGe Navigator) for relevant studies with well-defined inclusion and exclusion criteria and assessed the risk of bias using novel Confounding-Selection-Information bias score (CSI). RESULTS: 5105 studies were initially identified, but only 5 met all the inclusion criteria and were analyzed in detail...
August 31, 2016: Croatian Medical Journal
Xinfang Dong, Yanping Luo, Qi Gao, Xiaoling Lu, Qian Wang, Yuan Zhang, Xun Liu, Lifeng Zhang, Jingqiu Wang, Xingming Ma, Bingdong Zhu
Tuberculosis is a chronic infectious disease, which caused by Mycobacterium tuberculosis. It typically affects the functions of the lung and causes high morbidity and mortality rates worldwide. The lectin pathway, one of the complement cascade systems, provides the primary line of defense against invading pathogens. However, what is the specific effection between tuberculosis and complement is unknown. Mannose-binding lectin (MBL), a recognition subunit, binds to arrays of carbohydrates on the surfaces of pathogens, which results in the activation of MBL-associated serine protease-2 to trigger a downstream reaction cascade of complement system...
October 2016: Microbial Pathogenesis
Yong Liu, Ning-Qiu Li, Xin-Peng Zhao, Bin Yue, Shu-Wen He, Zhi-Xin Gao, Shun Zhou, Min Zhang
C-type lectins (CTLs) are important pattern recognition receptors (PRRs) that play vital roles in innate immunity. In teleosts, a number of CTLs have been reported, but their in vivo effects on host defense are still limited. In this study, a CTL homolog (SsLec1) was identified from black rockfish, Sebastes schlegelii, and its structure, expression and biological function was analyzed. The open reading frame of SsLec1 is 633 bp, with a 5'- untranslated region (UTR) of 36 bp and a 3'- UTR of 117 bp. The deduced amino acid sequence of SsLec1 shares the highest overall identity (73...
October 2016: Fish & Shellfish Immunology
Sakihito Kitajima, Kenji Miura, Wataru Aoki, Katsuyuki T Yamato, Toki Taira, Ryuta Murakami, Shunsuke Aburaya
The cytoplasm of laticifers, which are plant cells specialized for rubber production and defense against microbes and herbivores, is a latex. Although laticifers share common functions, the protein constituents of latexes are highly variable among plant species and even among organs. In this study, transcriptomic and proteomic analyses of Euphorbia tirucalli's (Euphorbiaceae) latex were conducted to determine the molecular basis of the laticifer's functions in this plant. The hybrid de novo assembly of Illumina mRNA-seq and expressed sequence tags obtained by Sanger's sequencing revealed 26,447 unigenes...
August 18, 2016: Plant Physiology and Biochemistry: PPB
Patricia R Pereira, Jennifer L Meagher, Harry C Winter, Irwin J Goldstein, Vânia M F Paschoalin, Joab T Silva, Jeanne A Stuckey
Tarin, the Colocasia esculenta lectin from the superfamily of α-d-mannose-specific plant bulb lectins, is a tetramer of 47 kDa composed of two heterodimers. Each heterodimer possesses homologous monomers of ~11.9 (A chain) and ~12.7 (B chain) kDa. The structures of apo and carbohydrate-bound tarin were solved to 1.7 Å and 1.91 Å, respectively. Each tarin monomer forms a canonical β-prism II fold, common to all members of Galanthus nivalis agglutinin (GNA) family, which is partially stabilized by a disulfide bond and a conserved hydrophobic core...
August 24, 2016: Glycobiology
Felix Poppelaars, Mariana Gaya da Costa, Stefan P Berger, Solmaz Assa, Anita H Meter-Arkema, Mohamed R Daha, Willem J van Son, Casper F M Franssen, Marc A J Seelen
No abstract text is available yet for this article.
2016: Journal of Translational Medicine
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