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https://www.readbyqxmd.com/read/28551114/a-mwcnts-pt-nanohybrids-based-highly-sensitive-electrochemiluminescence-sensor-for-flavonoids-assay
#1
Hong Ke, Xin Zhang, Weiwei Guo, Amin Zhang, Zhiming Wang, Chusen Huang, Nengqin Jia
Electrochemiluminescent (ECL) assay has gradually drawn increasing interest to be considered as one of the potential strategies to be applicable in a wide range of medicine target determination. In this protocol, a facile and ultrasensitive ECL sensor platform was developed aiming at rapidly quantitative detection of polyphenolic flavonoids based on multiwall carbon nanotubes (MWCNTs)/platinum nanoparticles (PtNPs) nanohybrids/nafion/Ru(bpy)3(2+) modified glassy carbon electrode (GCE) in Ru(bpy)3(2+)/tri-n-propylamine (TPA) co-reaction system...
August 15, 2017: Talanta
https://www.readbyqxmd.com/read/28551055/fresh-osteochondral-allografts-procurement-and-tissue-donation-in-europe
#2
S Schmidt, A Schulte, S Schwarz, N Hofmann, S Tietz, M Boergel, S U Sixt
Fresh osteochondral allografts are a well-established treatment for large, full-thickness cartilage defects. The clinical outcome for carefully selected patients is very favorable, especially for the young and active and graft survival up to 25 years has been described in the literature. Furthermore, a high patient satisfaction rate has been reported, but the biggest obstacle to overcome is the availability of tissue for transplantation. Large fresh bone allografts for cartilage damage repair only can be harvested from organ donors following organ removal or cadaveric donors, preferably in the setting of an operation room to minimize possible contamination of the tissue...
May 11, 2017: Injury
https://www.readbyqxmd.com/read/28550979/status-of-blood-transfusion-in-world-health-organization-eastern-mediterranean-region-who-emr-successes-and-challenges
#3
REVIEW
Arezoo Darbandi, Pargol Mashati, Amir Yami, Arshia Gharehbaghian, Mehdi Tabrizi Namini, Ahmad Gharehbaghian
BACKGROUND: Blood products are used for patient treatment and survival in the cases of major surgery, hematological disorders or cancer therapy. Presently the main blood components are not yet replaceable by artificial products and all activities related to blood transfusion is highly dependent on the healthcare development of each country. The World Health Organization Eastern Mediterranean Region (WHO-EMR) comprises of 21 member states with variable socio-economic status effective on blood transfusion activities...
May 8, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28550713/state-of-the-art-on-oocyte-cryopreservation-in-female-cancer-patients-a-critical-review-of-the-literature
#4
REVIEW
Claudia Massarotti, Paola Scaruffi, Matteo Lambertini, Valentino Remorgida, Lucia Del Mastro, Paola Anserini
During the last decades, important advances in therapeutic options have led to increased survival rates in cancer patients; however, cancer treatments are associated with several potential adverse effects including infertility in those diagnosed during their reproductive years. A proper discussion about fertility preservation options before the use of therapies with potential gonadotoxicity (i.e. oncofertility counseling) is standard of care and should be offered to all patients of childbearing age. Temporary ovarian suppression with LH-RH analogs, oocyte and embryo cryopreservation are standard strategies for fertility preservation in female cancer patients...
May 9, 2017: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/28549594/-recommendations-for-the-organ-donation-from-patients-with-brain-or-medullary-primitive-tumors-on-behalf-of-the-association-of-the-neuro-oncologists-of-french-expression-anocef-and-the-club-of-neuro-oncology-of-the-french-society-of-neurosurgery
#5
Didier Frappaz, Emilie Le Rhun, Arnaud Dagain, Benoît Averland, Luc Bauchet, Alexandre Faure, Christian Guillaume, Sonia Zouaoui, François Provot, Florence Vachiery, Luc Taillandier, Khê Hoang-Xuan
Requests of organs to be transplanted increase. As a matter of urgency, it is not always easy to decide if a patient carrier of a brain tumor can be candidate in the donation. After a review of the literature, the members of the Association of the Neuro-oncologists of French Expression (ANOCEF) and the Club of Neuro-oncology of the French Society of Neurosurgery propose consensual recommendations in case of donor carrier of primitive tumor intra-cranial or intra-medullary. A contact with the neuro-oncologist/neurosurgeon will allow to discuss the indication in case of glioma of grade I/II/III, according to the grade, the current status (absence of progressive disease), the number of surgeries and of lines of treatment...
May 23, 2017: Bulletin du Cancer
https://www.readbyqxmd.com/read/28549399/a-systematic-review-investigating-psychosocial-aspects-of-egg-sharing-in-the-united-kingdom-and-their-potential-effects-on-egg-donation-numbers
#6
Timothy Bracewell-Milnes, Srdjan Saso, Hossam Abdalla, Meen-Yau Thum
This review aims to provide an up-to-date knowledge of the psychosocial aspects of egg donation from the perspectives of the egg share donor and their recipient. It explores the motives, experiences and attitudes of egg sharers and their views towards donor anonymity and disclosure. Conclusions are made on how these findings can guide clinical practice and improve egg sharing numbers. A systematic search of peer-reviewed journals of four computerized databases was undertaken. Eleven studies were included in the review...
May 26, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28548666/zinc-and-the-iron-donor-frataxin-regulate-oligomerization-of-the-scaffold-protein-to-form-new-fe-s-cluster-assembly-centers
#7
B K Galeano, W Ranatunga, O Gakh, D Y Smith, J R Thompson, G Isaya
Early studies of the bacterial Fe-S cluster assembly system provided structural details for how the scaffold protein and the cysteine desulfurase interact. This work and additional work on the yeast and human systems elucidated a conserved mechanism for sulfur donation but did not provide any conclusive insights into the mechanism for iron delivery from the iron donor, frataxin, to the scaffold. We previously showed that oligomerization is a mechanism by which yeast frataxin (Yfh1) can promote assembly of the core machinery for Fe-S cluster synthesis both in vitro and in cells, in such a manner that the scaffold protein, Isu1, can bind to Yfh1 independent of the presence of the cysteine desulfurase, Nfs1...
May 26, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28548609/a-pilot-study-to-validate-measures-of-the-theory-of-reasoned-action-for-organ-donation-behavior
#8
Shui Hung Wong, Amy Yin Man Chow
The present study aimed at taking the first attempt in validating the measures generated based on the theory of reasoned action (TRA). A total of 211 university students participated in the study, 95 were included in the exploratory factor analysis and 116 were included in the confirmatory factor analysis. The TRA measurements were established with adequate psychometric properties, internal consistency and construct validity. Findings also suggested that attitude towards organ donation has both a cognitive and affective nature, while the subjective norm of the family seems to be important to students' views on organ donation...
May 26, 2017: Death Studies
https://www.readbyqxmd.com/read/28547759/blood-donors-attitudes-towards-voluntary-non-remunerated-donation-in-trinidad-and-tobago
#9
K S Charles, A Poon King, A Ramai, K Rajnath, D Ramkissoon, S Ramkissoon, C Ramlal, K Ramnarine, K Rampersad, G Legall, S Pooransingh, A D Chantry
OBJECTIVES: To assess the attitude towards voluntary non-remunerated blood donation among blood donors in Trinidad and Tobago (TRT). BACKGROUND: Blood donors in TRT are either family replacement (F/R, 87%) or remunerated (13%). There is chronic blood shortage and high seroreactivity for transfusion-transmissible infections (TTI) in donors. Converting existing to voluntary non-remunerated donors (VNRD) reduces the need to recruit news donors in achieving 100% VNRD...
May 26, 2017: Transfusion Medicine
https://www.readbyqxmd.com/read/28546462/alternative-donor-graft-sources-for-adults-with-hematologic-malignancies-a-donor-for-all-patients-in-2017
#10
REVIEW
Tamila L Kindwall-Keller, Karen K Ballen
Hematopoietic stem cell transplant (HSCT) is potentially curative for a wide variety of malignant diseases, including acute and leukemias, lymphoma, and myelodysplasia. Choice of a stem cell donor is dependent on donor availability, donor compatibility and health, recipient disease type, and recipient condition. Current sources of stem cell donation for HSCT are matched sibling donors (MSDs), matched unrelated donors (MUDs), 1-antigen mismatched unrelated donors (MMUDs), haploidentical donors (haplo), and umbilical cord blood (UCB) units...
May 25, 2017: Oncologist
https://www.readbyqxmd.com/read/28546425/the-first-crystal-structure-of-a-family-129-glycoside-hydrolase-from-a-probiotic-bacterium-reveals-critical-residues-and-metal-co-factors
#11
Mayo Sato, Dorothee Liebschner, Yusuke Yamada, Naohiro Matsugaki, Takatoshi Arakawa, Siobhan S Wills, Mitchell Hattie, Keith A Stubbs, Tasuku Ito, Toshiya Senda, Hisashi Ashida, Shinya Fushinobu
The α-N-acetylgalactosaminidase from the probiotic bacterium Bifidobacterium bifidum (NagBb) belongs to the glycoside hydrolase (GH) family 129 and hydrolyzes the glycosidic bond of Tn antigen (GalNAcα1-Ser/Thr). NagBb is involved in assimilation of O-glycans on mucin glycoproteins by B. bifidum in the human gastrointestinal tract, but its catalytic mechanism has remained elusive because of a lack of sequence homology around putative catalytic residues and of other structural information. Here we report the X-ray crystal structure of NagBb, representing the first GH129 family structure, solved by the single-wavelength anomalous dispersion method based on sulfur atoms of the native protein...
May 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28545474/a-retrospective-study-to-assess-the-evaluation-of-living-related-kidney-donors-and-their-outcomes-following-nephrectomy-at-kenyatta-national-hospital
#12
A Muturi, V Kotecha, S Kanyi
BACKGROUND: Kidney transplantation is the renal replacement therapy of choice for end stage renal disease. To ensure safety regular audit of the donation process is necessary. The aim of this study was to assess the evaluation of potential living related kidney donors and document their outcomes following nephrectomy. METHODS: This was a retrospective descriptive study involving all living related kidney donors seen at Kenyatta National Hospital (KNH) renal unit from 2010 to 2014...
May 25, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28545163/coccygeal-body-revisited-an-immunohistochemical-study-using-donated-elderly-cadavers
#13
Zhe Wu Jin, Kwang Ho Cho, Hyung Suk Jang, Gen Murakami, Jose Francisco Rodríguez-Vázquez, Masahito Yamamoto, Shin-Ichi Abe
To describe the normal anatomy and histology of the adult coccygeal body (CB) and to discuss about the origin and function. Using immunohistochemistry, we examined 29 CBs found in 32 elderly donated cadavers without macroscopic pathology in the pelvis. The CB was usually located in or near the anococcygeal ligaments. It was almost always composed of multiple masses or nodules of round glomus cells (smooth muscle actin, or SMA++). However, the CB sometimes contained abundant dilated veins with scattered glomus cells...
May 25, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28544420/gold-i-hydrides-as-proton-acceptors-in-dihydrogen-bond-formation
#14
Ignacy Cukrowski, Jurgens Hendrik de Lange, Ferdinand Groenewald, Helgard G Raubenheimer
Wavefunction and DFT calculations indicate that anionic dihydride complexes of AuI form strong to moderate directed Au-H···H bonds with one or two HF, H₂O and NH₃ prototype proton donor molecules. The largely electrostatic interaction is influenced by relativistic effects which, however, do not increase the binding energy. Very weak Au···H associations - exhibiting a corresponding bond path - occur between neutral AuH and HF units, although ultimately F becomes the preferred donor atom in the most stable structure...
May 25, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28544308/use-of-a-new-end-stage-kidney-disease-risk-calculator-in-the-kidney-disease-improving-global-outcomes-guideline-to-evaluate-the-impact-of-different-living-kidney-donor-candidate-assessments
#15
Darren Lee, Momena Manzoor, Geoff Harley, John Whitlam, Natasha Cook, Suet-Wan Choy, Megan Sandiford, Charlotte Gibson, Lawrence P McMahon, Matthew A Roberts
AIM: The Kidney Disease Improving Global Outcomes (KDIGO) guideline recommends the incorporation of a new risk calculator that quantifies the end stage kidney disease (ESKD) risk based on a composite profile of risk factors in living kidney donor candidates (LKDCs). We compared the ESKD risk estimates in previously declined versus accepted LKDCs to evaluate the predictive capacity and potential impact of this tool. METHODS: Baseline 15-year and lifetime ESKD risk estimates without donation were calculated using the risk calculator for LKDCs assessed from two centres between 2007 and 2015...
May 20, 2017: Nephrology
https://www.readbyqxmd.com/read/28544228/synthesis-of-benzodihydrofurans-by-asymmetric-c-h-insertion-reactions-of-donor-donor-carbenoids
#16
Jared Thomas Shaw, Kellan N Lamb, Richard A Squitieri, Srinivasa R Chintala, Ada J Kwong, Edward I Balmond, Cristian Soldi, Olga Dmytrenko, Marta Castiñeira Reis, Ryan Chung, J Bennett Addison, James C Fettinger, Jason C Hein, Dean J Tantillo, Joseph M Fox
Metal carbenoids appended with two electron-donating groups, known as "donor/donor" carbenoids, undergo diastereo- and enantioselective rhodium-catalyzed C-H insertion reactions with ether substrates to form benzodihydrofurans. Unlike the reactions of carbenoids with electron-withdrawing groups attached, the attenuated electrophilicity enables these reactions to be conducted in Lewis basic solvents (e.g., acetonitrile) and in the presence of water. The diazo precursors for these species are prepared in situ from hydrazone using a mild and chemoselective oxidant (MnO2)...
May 22, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28544131/sclerocorneal-graft-and-sequential-removal-of-melted-cornea-after-severe-corneal-burn-with-perforation
#17
Anja Viestenz, Berthold Seitz, Hans-Gert Struck, Arne Viestenz
INTRODUCTION: Corneal burn grade IV usually leads to blindness. Several different surgical techniques remain challenging owing to the extensive tissue damage. Here, we introduce a novel technique with a 15 mm corneoscleral and limbal homologous graft combined with sequential autologous corneal removal ab interno, with a vitrectomy probe to save the anterior chamber angle. In vivo anatomy with optical coherence tomography is the surgical key. METHOD: A large 15 mm sclerocorneal graft is sutured on top of the remainder of the destroyed cornea and sclera after removal of the epithelium and conjunctiva, with anterior synechiolysis if necessary, peripheral iridectomy and conjunctivoplasty...
May 22, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28544047/comparison-of-oral-fluid-collection-methods-for-the-molecular-detection-of-hepatitis-b-virus
#18
Moyra Machado Portilho, Ana Carolina da Fonseca Mendonça, Vanessa Alves Marques, Leticia Cancella Nabuco, Cristiane Alves Villela-Nogueira, Claudia Ivantes, Lia Laura Lewis-Ximenez, Elisabeth Lampe, Livia Melo VIllar
BACKGROUND: This study aims to compare the efficiency of four oral fluid collection methods (Salivette, FTA Card, spitting and DNA-Sal) to detect HBV DNA by qualitative PCR. MATERIALS AND METHODS: Seventy-four individuals (32 HBV reactive and 42 with no HBV markers) donated serum and oral fluid. In-house qualitative PCR to detect HBV was used for both samples and commercial quantitative PCR for serum. RESULTS: HBV DNA was detected in all serum samples from HBV-infected individuals, and it was not detected in control group...
May 19, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28543884/psychological-factors-related-to-donation-behaviour-among-chinese-adults-results-from-a-longitudinal-investigation
#19
H Hu, T Wang, Q Fu
BACKGROUND AND OBJECTIVES: Little is known about the psychological factors currently influencing blood donation in China. This study investigated the structure of psychological factors and their correlation with donation behaviour of adults in a transforming city in China over a 6-month period. METHODS: Participants were recruited in Nanjing from May 2013 to April 2014. Preliminary focus group interviews with 102 participants were conducted to generate new items for a Theory of Planned Behaviour (TPB) questionnaire...
May 22, 2017: Transfusion Medicine
https://www.readbyqxmd.com/read/28543832/an-investigation-of-the-impact-of-melbourne-zoo-s-seal-the-loop-donate-call-to-action-on-visitor-satisfaction-and-behavior
#20
Sarah Mellish, Ben Sanders, Carla A Litchfield, Elissa L Pearson
Modern zoos are uniquely positioned to educate the public about environmental issues and promote conservation action. This report investigates the introduction of a donation request during an interactive fur seal presentation (as part of Melbourne Zoo's "Seal-the-Loop" initiative) on visitor satisfaction, perceptions of donation as a way to help wild fur seals, and donation behaviors. Comparisons are made between three groups surveyed upon exit: (1) viewed the interactive fur seal presentation prior to the donation request implementation (pledge-presentation: N = 86; see Mellish, Pearson, Sanders, and Litchfield []; International Zoo Yearbook 129:129-154); (2) viewed the interactive fur seal presentation including the donation request (donate-presentation: N = 82); and (3) viewed the fur seal exhibit and donation point but not the presentation and were not directly asked to make a donation (donate-exhibit: N = 82)...
May 23, 2017: Zoo Biology
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