keyword
MENU ▼
Read by QxMD icon Read
search

Reinhardtii

keyword
https://www.readbyqxmd.com/read/28815214/chlamydomonas-reinhardtii-lfo1-is-an-isdg-family-heme-oxygenase
#1
Lisa J Lojek, Allison J Farrand, Jennifer H Wisecaver, Crysten E Blaby-Haas, Brian W Michel, Sabeeha S Merchant, Antonis Rokas, Eric P Skaar
Heme is essential for respiration across all domains of life. However, heme accumulation can lead to toxicity if cells are unable to either degrade or export heme or its toxic by-products. Under aerobic conditions, heme degradation is performed by heme oxygenases, enzymes which utilize oxygen to cleave the tetrapyrrole ring of heme. The HO-1 family of heme oxygenases has been identified in both bacterial and eukaryotic cells, whereas the IsdG family has thus far been described only in bacteria. We identified a hypothetical protein in the eukaryotic green alga Chlamydomonas reinhardtii, which encodes a protein containing an antibiotic biosynthesis monooxygenase (ABM) domain consistent with those associated with IsdG family members...
July 2017: MSphere
https://www.readbyqxmd.com/read/28812653/adaptation-of-phytoplankton-to-a-decade-of-experimental-warming-linked-to-increased-photosynthesis
#2
C-Elisa Schaum, Samuel Barton, Elvire Bestion, Angus Buckling, Bernardo Garcia-Carreras, Paula Lopez, Chris Lowe, Samraat Pawar, Nicholas Smirnoff, Mark Trimmer, Gabriel Yvon-Durocher
Phytoplankton photosynthesis is a critical flux in the carbon cycle, accounting for approximately 40% of the carbon dioxide fixed globally on an annual basis and fuelling the productivity of aquatic food webs. However, rapid evolutionary responses of phytoplankton to warming remain largely unexplored, particularly outside the laboratory, where multiple selection pressures can modify adaptation to environmental change. Here, we use a decade-long experiment in outdoor mesocosms to investigate mechanisms of adaptation to warming (+4 °C above ambient temperature) in the green alga Chlamydomonas reinhardtii, in naturally assembled communities...
March 20, 2017: Nature ecology & evolution
https://www.readbyqxmd.com/read/28810200/influences-of-tio2-nanoparticles-on-dietary-metal-uptake-in-daphnia-magna
#3
Cheng Tan, Wen-Xiong Wang
Increasing applications of titanium dioxide nanoparticles (nano-TiO2) have intensified the risk of environmental contamination. Since nano-TiO2 can absorb metals and be consumed as 'food' by zooplankton but also can interact with phytoplankton, they could significantly disturb the existing metal assimilation patterns. In the present study, we quantified the dietary assimilation of Cd and Zn from nano-TiO2 and algae (Chlamydomonas reinhardtii) at comparable particle concentrations as well as in complex food environment (variable food quality and quantity) in a freshwater zooplankton Daphnia magna using the radiotracer technique...
August 11, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28808314/cellular-toxicity-pathways-of-inorganic-and-methyl-mercury-in-the-green-microalga-chlamydomonas-reinhardtii
#4
Rebecca Beauvais-Flück, Vera I Slaveykova, Claudia Cosio
Contamination by mercury (Hg) is a worldwide concern because of Hg toxicity and biomagnification in aquatic food webs. Nevertheless, bioavailability and cellular toxicity pathways of inorganic (IHg) and methyl-Hg (MeHg) remain poorly understood. We analyzed the uptake, transcriptomic, and physiological responses in the microalga Chlamydomonas reinhardtii exposed to IHg or MeHg. Bioavailability of MeHg was up to 27× higher than for IHg. Genes involved in cell processes, energy metabolism and transport were dysregulated by both Hg species...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28798763/maturation-of-plastid-c-type-cytochromes
#5
REVIEW
Stéphane T Gabilly, Patrice P Hamel
Cytochromes c are hemoproteins, with the prosthetic group covalently linked to the apoprotein, which function as electron carriers. A class of cytochromes c is defined by a CXXCH heme-binding motif where the cysteines form thioether bonds with the vinyl groups of heme. Plastids are known to contain up to three cytochromes c. The membrane-bound cytochrome f and soluble cytochrome c6 operate in photosynthesis while the activity of soluble cytochrome c6A remains unknown. Conversion of apo- to holocytochrome c occurs in the thylakoid lumen and requires the independent transport of apocytochrome and heme across the thylakoid membrane followed by the stereospecific attachment of ferroheme via thioether linkages...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28781053/identification-of-chlamydomonas-central-core-centriolar-proteins-reveals-a-role-for-human-wdr90-in-ciliogenesis
#6
Virginie Hamel, Emmanuelle Steib, Romain Hamelin, Florence Armand, Susanne Borgers, Isabelle Flückiger, Coralie Busso, Natacha Olieric, Carlos Oscar S Sorzano, Michel O Steinmetz, Paul Guichard, Pierre Gönczy
Centrioles are evolutionarily conserved macromolecular structures that are fundamental to form cilia, flagella, and centrosomes. Centrioles are 9-fold symmetrical microtubule-based cylindrical barrels comprising three regions that can be clearly distinguished in the Chlamydomonas reinhardtii organelle: an ∼100-nm-long proximal region harboring a cartwheel; an ∼250-nm-long central core region containing a Y-shaped linker; and an ∼150-nm-long distal region ending at the transitional plate. Despite the discovery of many centriolar components, no protein has been localized specifically to the central core region in Chlamydomonas thus far...
July 29, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28775289/reaction-dynamics-of-the-chimeric-channelrhodopsin-c1c2
#7
Yusaku Hontani, Marco Marazzi, Katja Stehfest, Tilo Mathes, Ivo H M van Stokkum, Marcus Elstner, Peter Hegemann, John T M Kennis
Channelrhodopsin (ChR) is a key protein of the optogenetic toolkit. C1C2, a functional chimeric protein of Chlamydomonas reinhardtii ChR1 and ChR2, is the only ChR whose crystal structure has been solved, and thus uniquely suitable for structure-based analysis. We report C1C2 photoreaction dynamics with ultrafast transient absorption and multi-pulse spectroscopy combined with target analysis and structure-based hybrid quantum mechanics/molecular mechanics calculations. Two relaxation pathways exist on the excited (S1) state through two conical intersections CI1 and CI2, that are reached via clockwise and counter-clockwise rotations: (i) the C13=C14 isomerization path with 450 fs via CI1 and (ii) a relaxation path to the initial ground state with 2...
August 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28751903/evolution-of-daily-gene-co-expression-patterns-from-algae-to-plants
#8
Pedro de Los Reyes, Francisco J Romero-Campero, M Teresa Ruiz, José M Romero, Federico Valverde
Daily rhythms play a key role in transcriptome regulation in plants and microalgae orchestrating responses that, among other processes, anticipate light transitions that are essential for their metabolism and development. The recent accumulation of genome-wide transcriptomic data generated under alternating light:dark periods from plants and microalgae has made possible integrative and comparative analysis that could contribute to shed light on the evolution of daily rhythms in the green lineage. In this work, RNA-seq and microarray data generated over 24 h periods in different light regimes from the eudicot Arabidopsis thaliana and the microalgae Chlamydomonas reinhardtii and Ostreococcus tauri have been integrated and analyzed using gene co-expression networks...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28742931/dynamics-of-lipids-and-metabolites-during-the-cell-cycle-of-chlamydomonas-reinhardtii
#9
Jessica Jüppner, Umarah Mubeen, Andrea Leisse, Camila Caldana, Henrike Brust, Martin Steup, Marion Herrmann, Dirk Steinhauser, Patrick Giavalisco
Metabolites and lipids are the final products of enzymatic processes, distinguishing the different cellular functions and activities of single cells or whole tissues. Understanding these cellular functions within a well-established model system, requires a systemic collection of molecular and physiological information. In the current report, the green algae Chlamydomonas reinhardtii was selected to establish a comprehensive workflow for the detailed multi-omics analysis of a synchronously growing cell culture system...
July 25, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28739495/the-deep-thioredoxome-in-chlamydomonas-reinhardtii-new-insights-into-redox-regulation
#10
María Esther Pérez-Pérez, Adeline Mauriès, Alexandre Maes, Nicolas J Tourasse, Marion Hamon, Stéphane D Lemaire, Christophe H Marchand
Thiol-based redox post-translational modifications have emerged as important mechanisms of signaling and regulation in all organisms, and thioredoxin plays a key role by controlling the thiol-disulfide status of target proteins. Recent redox proteomic studies revealed hundreds of proteins regulated by glutathionylation and nitrosylation in the unicellular green alga Chlamydomonas reinhardtii, while much less is known about the thioredoxin interactome in this organism. By combining qualitative and quantitative proteomic analyses, we have comprehensively investigated the Chlamydomonas thioredoxome and 1188 targets have been identified...
August 7, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28718986/the-effect-of-selection-history-on-extinction-risk-during-severe-environmental-change
#11
Josianne Lachapelle, Nick Colegrave, Graham Bell
Environments rarely remain the same over time, and populations are therefore frequently at risk of going extinct when changes are significant enough to reduce fitness. While many studies have investigated what attributes of the new environments and of the populations experiencing these changes will affect their probability of going extinct, limited work has been directed toward determining the role of population history on the probability of going extinct during severe environmental change. Here we compare the extinction risk of populations with a history of selection in a benign environment, to populations with a history of selection in one or two stressful environments...
July 18, 2017: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/28710366/mirnas-in-the-alga-chlamydomonas-reinhardtii-are-not-phylogenetically-conserved-and-play-a-limited-role-in-responses-to-nutrient-deprivation
#12
Adam Voshall, Eun-Jeong Kim, Xinrong Ma, Tomohito Yamasaki, Etsuko N Moriyama, Heriberto Cerutti
The unicellular alga Chlamydomonas reinhardtii contains many types of small RNAs (sRNAs) but the biological role(s) of bona fide microRNAs (miRNAs) remains unclear. To address their possible function(s) in responses to nutrient availability, we examined miRNA expression in cells cultured under different trophic conditions (mixotrophic in the presence of acetate or photoautotrophic in the presence or absence of nitrogen). We also reanalyzed miRNA expression data in Chlamydomonas subject to sulfur or phosphate deprivation...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710131/gene-regulatory-networks-for-the-haploid-to-diploid-transition-of-chlamydomonas-reinhardtii
#13
Sunjoo Joo, Yoshiki Nishimura, Evan Cronmiller, Ran Ha Hong, Thamali Kariyawasam, Ming Hsiu Wang, Nai Chun Shao, Saif-El-Din El Akkad, Takamasa Suzuki, Tetsuya Higashiyama, EonSeon Jin, Jae-Hyeok Lee
The sexual cycle of the unicellular Chlamydomonas reinhardtii culminates in the formation of diploid zygotes which differentiate into dormant spores that eventually undergo meiosis. Mating between gametes induces rapid cell wall shedding via the enzyme g-lysin; cell fusion is followed by heterodimerization of sex-specific homeobox transcription factors, GSM1 and GSP1, and initiation of zygote-specific gene expression. To investigate the genetic underpinnings of the zygote developmental pathway, we performed comparative transcriptome analysis of both pre- and post-fertilization samples...
July 14, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28708270/transcriptional-and-cellular-effects-of-benzotriazole-uv-stabilizers-uv-234-and-uv-328-in-the-freshwater-invertebrates-chlamydomonas-reinhardtii-and-daphnia-magna
#14
Maeva Giraudo, Guillaume Cottin, Marta Esperanza, Pierre Gagnon, Amila O De Silva, Magali Houde
Benzotriazole UV-stabilizers (BZT-UVs) are compounds used in many applications and products to prevent photochemical degradation. Despite their widespread presence in aquatic ecosystems and persistence in the environment, there are very limited data on their effects and toxicity, and their modes of action remain largely unknown. The objectives of this study were to evaluate the chronic effects of two BZT-UVs, 2-(2H-benzotriazol-2-yl)-4,6-bis(1-methyl-1-phenylethyl)phenol (UV-234) and 2-(2H-benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV-328), on the freshwater green algae Chlamydomonas reinhardtii and the freshwater crustacean Daphnia magna...
July 14, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28706759/mild-and-selective-protein-release-of-cell-wall-deficient-microalgae-with-pulsed-electric-field
#15
Gerard P 't Lam, Jelmer A van der Kolk, Akshita Chordia, Marian H Vermuë, Giuseppe Olivieri, Michel H M Eppink, René H Wijffels
Pulsed electric field (PEF) is considered to be a very promising technology for mild cell disruption. The application of PEF for microalgae that have a rigid cell wall, however, is hampered by the presence of that rigid outer cell wall. A cell wall free mutant of C. reinhardtii was used to mimic pretreated microalgae with removed cell wall, to investigate the possibility of using PEF for protein release from microalgae. A complete release of hydrophilic proteins from the cell wall free mutants was observed whereas PEF treatment on the cell wall containing species resulted in substantially lower protein yields...
July 3, 2017: ACS Sustainable Chemistry & Engineering
https://www.readbyqxmd.com/read/28704927/the-tor-signaling-network-in-the-model-unicellular-green-alga-chlamydomonas-reinhardtii
#16
REVIEW
María Esther Pérez-Pérez, Inmaculada Couso, José L Crespo
Cell growth is tightly coupled to nutrient availability. The target of rapamycin (TOR) kinase transmits nutritional and environmental cues to the cellular growth machinery. TOR functions in two distinct multiprotein complexes, termed TOR complex 1 (TORC1) and TOR complex 2 (TORC2). While the structure and functions of TORC1 are highly conserved in all eukaryotes, including algae and plants, TORC2 core proteins seem to be missing in photosynthetic organisms. TORC1 controls cell growth by promoting anabolic processes, including protein synthesis and ribosome biogenesis, and inhibiting catabolic processes such as autophagy...
July 12, 2017: Biomolecules
https://www.readbyqxmd.com/read/28703942/low-temperature-and-phosphate-deficiency-responsive-elements-control-dgtt3-expression-in-chlamydomonas-reinhardtii
#17
Xiaowen Fei, Ping Li, Xinghan Li, Xiaodong Deng
acyl-CoA:Diacylglycerol acyltransferases (DGAT) catalyse the final step of the triacylglycerol biosynthesis. Two major gene families have been shown to encode DGATs, DGAT1 and DGAT2. Abiotic factors such as low temperatures, nitrogen or phosphorus deficiency was reported to play important roles in the growth and development in green algae. Whether DGATs are induced by low temperatures or phosphorus deficiency, and the corresponding promoter elements are not reported yet. In this study, we found DGTT3 to have a significant response to low temperatures, phosphorus deficiency, and other stresses, such as high concentrations of NaCl, 20 μM GA, and 20 μM abscisic acid...
July 13, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28693951/gene-silencing-of-stearoyl-acp-desaturase-enhances-the-stearic-acid-content-in-chlamydomonas-reinhardtii
#18
L de Jaeger, J Springer, E J H Wolbert, D E Martens, G Eggink, R H Wijffels
In this study, stearoyl-ACP desaturase (SAD), the enzyme that converts stearic acid into oleic acid, is silenced by artificial microRNA in the green microalga Chlamydomonas reinhardtii. Two different constructs, which target different positions on the mRNA of stearoyl-ACP desaturase, were tested. The mRNA levels for SAD were reduced after the silencing construct was induced. In one of the strains, the reduction in SAD mRNA resulted in a doubling of the stearic acid content in triacylglycerol molecules, which shows that stearic acid production in microalgae is possible...
June 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28692856/nutrient-scavenging-and-energy-management-acclimation-responses-in-nitrogen-and-sulfur-deprived-chlamydomonas
#19
REVIEW
Shai Saroussi, Emanuel Sanz-Luque, Rick G Kim, Arthur R Grossman
Photosynthetic organisms have evolved to modulate their metabolism to accommodate the highly dynamic light and nutrient conditions in nature. In this review we discuss ways in which the green alga Chlamydomonas reinhardtii acclimates to nitrogen and sulfur deprivation, conditions that would limit the anabolic use of excitation energy because of a markedly reduced capacity for cell growth and division. Major aspects of this acclimation process are stringently regulated and involve scavenging the limited nutrient from internal and external sources, and the redirection of fixed carbon toward energy storage (e...
July 7, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28688014/comparative-proteomic-analysis-of-chlamydomonas-reinhardtii-control-and-a-salinity-tolerant-strain-revealed-a-differential-protein-expression-pattern
#20
Sayamon Sithtisarn, Kittisak Yokthongwattana, Bancha Mahong, Sittiruk Roytrakul, Atchara Paemanee, Narumon Phaonakrop, Chotika Yokthongwattana
Proteins involved in membrane transport and trafficking, stress and defense, iron uptake and metabolism, as well as proteolytic enzymes, were remarkably up-regulated in the salinity-tolerant strain of Chlamydomonas reinhardtii. Excessive concentration of NaCl in the environment can cause adverse effects on plants and microalgae. Successful adaptation of plants to long-term salinity stress requires complex cellular adjustments at different levels from molecular, biochemical and physiological processes. In this study, we developed a salinity-tolerant strain (ST) of the model unicellular green alga, Chlamydomonas reinhardtii, capable of growing in medium containing 300 mM NaCl...
July 7, 2017: Planta
keyword
keyword
87792
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"