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https://www.readbyqxmd.com/read/28647256/bioavailability-of-wastewater-derived-dissolved-organic-nitrogen-to-green-microalgae-selenastrum-capricornutum-chlamydomonas-reinhardtii-and-chlorella-vulgaris-with-without-presence-of-bacteria
#1
Jingyi Sun, Halis Simsek
Effluent dissolved organic nitrogen (DON) is problematic in nutrient sensitive surface waters and needs to be reduced to meet demanding total dissolved nitrogen discharge limits. Bioavailable DON (ABDON) is a portion of DON utilized by algae or algae+bacteria, while biodegradable DON (BDON) is a portion of DON decomposable by bacteria. ABDON and BDON in a two-stage trickling filter (TF) wastewater treatment plant was evaluated using three different microalgal species, Selenastrum capricornutum, Chlamydomonas reinhardtii and Chlorella vulgaris and mixed cultured bacteria...
July 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28646418/in-vivo-nmr-as-a-tool-for-probing-molecular-structure-and-dynamics-in-intact-chlamydomonas-reinhardtii-cells
#2
Fatemeh Azadi-Chegeni, Christo Schiphorst, Anjali Pandit
We report the application of NMR dynamic spectral editing for probing the structure and dynamics of molecular constituents in fresh, intact cells and in freshly prepared thylakoid membranes of Chlamydomonas reinhardtii (Cr.) green algae. For isotope labeling, wild-type Cr. cells were grown on (13)C acetate-enriched minimal medium. 1D (13)C J-coupling based and dipolar-based MAS NMR spectra were applied to distinguish (13)C resonances of different molecular components. 1D spectra were recorded over a physiological temperature range, and whole-cell spectra were compared to those taken from thylakoid membranes, evaluating their composition and dynamics...
June 23, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28645261/genome-wide-analysis-of-udp-glycosyltransferase-super-family-in-brassica-rapa-and-brassica-oleracea-reveals-its-evolutionary-history-and-functional-characterization
#3
Jingyin Yu, Fan Hu, Komivi Dossa, Zhaokai Wang, Tao Ke
BACKGROUND: Glycosyltransferases comprise a highly divergent and polyphyletic multigene family that is involved in widespread modification of plant secondary metabolites in a process called glycosylation. According to conserved domains identified in their amino acid sequences, these glycosyltransferases can be classified into a single UDP-glycosyltransferase (UGT) 1 superfamily. RESULTS: We performed genome-wide comparative analysis of UGT genes to trace evolutionary history in algae, bryophytes, pteridophytes, and angiosperms; then, we further investigated the expansion mechanisms and function characterization of UGT gene families in Brassica rapa and Brassica oleracea...
June 23, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28644828/a-mutant-of-chlamydomonas-without-lhcsr-maintains-high-rates-of-photosynthesis-but-has-reduced-cell-division-rates-in-sinusoidal-light-conditions
#4
Michael Cantrell, Graham Peers
The LHCSR protein belongs to the light harvesting complex family of pigment-binding proteins found in oxygenic photoautotrophs. Previous studies have shown that this complex is required for the rapid induction and relaxation of excess light energy dissipation in a wide range of eukaryotic algae and moss. The ability of cells to rapidly regulate light harvesting between this dissipation state and one favoring photochemistry is believed to be important for reducing oxidative stress and maintaining high photosynthetic efficiency in a rapidly changing light environment...
2017: PloS One
https://www.readbyqxmd.com/read/28641882/ferredoxin-dependent-bilin-reductases-in-eukaryotic-algae-ubiquity-and-diversity
#5
Nathan C Rockwell, J Clark Lagarias
Linear tetrapyrroles (bilins) are produced from heme by heme oxygenase, usually forming biliverdin IXα (BV). Fungi and bacteria use BV as chromophore for phytochrome photoreceptors. Oxygenic photosynthetic organisms use BV as a substrate for ferredoxin-dependent bilin reductases (FDBRs), enzymes that produce diverse reduced bilins used as light-harvesting pigments in phycobiliproteins and as photoactive photoreceptor chromophores. Bilin biosynthesis is essential for phototrophic growth in Chlamydomonas reinhardtii despite the absence of phytochromes or phycobiliproteins in this organism, raising the possibility that bilins are more generally required for phototrophic growth by algae...
May 31, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28638375/valorization-of-spent-escherichia-coli-media-using-green-microalgae-chlamydomonas-reinhardtii-and-feedstock-production
#6
Jian-Guo Zhang, Fang Zhang, Kiran Thakur, Fei Hu, Zhao-Jun Wei
The coupling of Chlamydomonas reinhardtii biomass production for nutrients removal of Escherichia coli anaerobic broth (EAB) is thought to be an economically feasible option for the cultivation of microalgae. The feasibility of growing microalgae in using EAB high in nutrients for the production of more biomass was examined. EAB comprised of nutrient-abundant effluents, which can be used to produce microalgae biomass and remove environment pollutant simultaneously. In this study, C. reinhardtii 21gr (cc1690) was cultivated in different diluted E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28637830/a-light-harvesting-complex-like-protein-in-maintenance-of-photosynthetic-components-in-chlamydomonas
#7
Lei Zhao, Dongmei Cheng, Xiahe Huang, Mei Chen, Luca Dall'Osto, Jiale Xing, Liyan Gao, Lingyu Li, Yale Wang, Roberto Bassi, Lianwei Peng, Yingchun Wang, Jean-David Rochaix, Fang Huang
Using a genetic approach we have identified and characterized a novel protein, named Msf1 (Maintenance Factor for PSI), which is required for the maintenance of specific components of the photosynthetic apparatus in the green alga Chlamydomonas reinhardtii. Msf1 belongs to the superfamily of light-harvesting complex (LHC) proteins with three trans-membrane domains and consensus chlorophyll binding sites. Loss of Msf1 leads to reduced accumulation of PSI and chlorophyll-binding proteins/complexes. Msf1 is a component of a thylakoid complex containing key enzymes of the tetrapyrrole biosynthetic pathway thus revealing a possible link between Msf1 and chlorophyll biosynthesis...
June 21, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28636910/prp40-homolog-a-is-a-novel-centrin-target
#8
Adalberto Díaz Casas, Walter J Chazin, Belinda Pastrana-Ríos
Pre-mRNA processing protein 40 (Prp40) is a nuclear protein that has a role in pre-mRNA splicing. Prp40 possesses two leucine-rich nuclear export signals, but little is known about the function of Prp40 in the export process. Another protein that has a role in protein export is centrin, a member of the EF-hand superfamily of Ca(2+)-binding proteins. Prp40 was found to be a centrin target by yeast-two-hybrid screening using both Homo sapiens centrin 2 (Hscen2) and Chlamydomonas reinhardtii centrin (Crcen). We identified a centrin-binding site within H...
June 20, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28633328/identification-and-characterization-of-a-solute-carrier-cia8-involved-in-inorganic-carbon-acclimation-in-chlamydomonas-reinhardtii
#9
Marylou C Machingura, Joanna Bajsa-Hirschel, Susan M Laborde, Joshua B Schwartzenburg, Bratati Mukherjee, Ananya Mukherjee, Steve V Pollock, Britta Förster, G Dean Price, James V Moroney
The supply of inorganic carbon (Ci) at the site of fixation by Rubisco is a key parameter for efficient CO2 fixation in aquatic organisms including the green alga, Chlamydomonas reinhardtii. Chlamydomonas reinhardtii cells, when grown on limiting CO2, have a CO2-concentrating mechanism (CCM) that functions to concentrate CO2 at the site of Rubisco. Proteins thought to be involved in inorganic carbon uptake have been identified and localized to the plasma membrane or chloroplast envelope. However, current CCM models suggest that additional molecular components are involved in Ci uptake...
June 13, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28630812/molecular-properties-of-a-dtd-channelrhodopsin-from-guillardia-theta
#10
Yumeka Yamauchi, Masae Konno, Shota Ito, Satoshi P Tsunoda, Keiichi Inoue, Hideki Kandori
Microbial rhodopsins are membrane proteins found widely in archaea, eubacteria and eukaryotes (fungal and algal species). They have various functions, such as light-driven ion pumps, light-gated ion channels, light sensors and light-activated enzymes. A light-driven proton pump bacteriorhodopsin (BR) contains a DTD motif at positions 85, 89, and 96, which is unique to archaeal proton pumps. Recently, channelrhodopsins (ChRs) containing the DTD motif, whose sequential identity is ~20% similar to BR and to cation ChRs in Chlamydomonas reinhardtii (CrCCRs), were found...
2017: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/28630648/comparative-metabolic-profiling-of-the-lipid-producing-green-microalga-chlorella-reveals-that-nitrogen-and-carbon-metabolic-pathways-contribute-to-lipid-metabolism
#11
Hui Chen, Yanli Zheng, Jiao Zhan, Chenliu He, Qiang Wang
Microalgae are a promising feedstock for biofuel production. Microalgal metabolic pathways are heavily influenced by environmental factors. For instance, lipid metabolism can be induced by nitrogen-limiting conditions. However, the underlying mechanisms of lipid biosynthesis are unclear. In this study, we analyzed the global metabolic profiles of three genetically closely related Chlorella strains (C1, C2, and C3) with significant differences in lipid productivity to identify the contributions of key metabolic pathways to lipid metabolism...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28628990/kinetic-modelling-of-starch-and-lipid-formation-during-mixotrophic-nutrient-limited-microalgal-growth
#12
Gonzalo M Figueroa-Torres, Jon K Pittman, Constantinos Theodoropoulos
Microalgal starch and lipids, carbon-based storage molecules, are useful as potential biofuel feedstocks. In this work, cultivation strategies maximising starch and lipid formation were established by developing a multi-parameter kinetic model describing microalgal growth as well as starch and lipid formation, in conjunction with laboratory-scale experiments. Growth dynamics are driven by nitrogen-limited mixotrophic conditions, known to increase cellular starch and lipid contents whilst enhancing biomass growth...
June 2, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28624997/expression-and-purification-of-cytochrome-p450-55b1-from-chlamydomonas-reinhardtii-and-its-application-in-nitric-oxide-biosensing
#13
Bin Gong, Xiaosheng Liang, Yong Li, Qian Xiao, Panchun Yang, Yunhua Wu
Cytochrome P450 55B1 from Chlamydomonas reinhardtii is reported to function as a nitric oxide reductase (NOR). Here, we expressed the cytochrome P450 55B1 gene with an HIS-tag in E scherichia coli using a pET28a vector. The native protein was produced at a level of 1.59 μmol/g of total protein, with approximately 85% of the P450 being soluble. The CYP55B1 protein was characterized spectrally and purified by a HIS-trap column. This procedure allowed recovery of 45% of the expressed protein and CYP55B1 with a specific content of 0...
June 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28623688/exposure-to-sublethal-concentrations-of-co3o4-and-mn2o3-nanoparticles-induced-elevated-metal-body-burden-in-daphnia-magna
#14
Margit Heinlaan, Marge Muna, Katre Juganson, Olena Oriekhova, Serge Stoll, Anne Kahru, Vera I Slaveykova
Despite the significant progress made in ecotoxicological research on nanoparticles (NPs), there is still very limited information available regarding the biological effects of certain types of NPs such as Co3O4 and Mn2O3. Only a couple of studies provide data on their impact on aquatic organisms whereas, alarmingly, these NPs have been proposed to have high toxicity potential. In addition, more data are needed to determine whether the adverse effects the metal NPs induce on aquatic organisms are rather due to their chemical or particulate nature...
June 6, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28620699/evolution-of-chlamydomonas-reinhardtii-ferredoxins-and-their-interactions-with-fefe-hydrogenases
#15
REVIEW
Anne Sawyer, Martin Winkler
Ferredoxins are soluble iron sulphur proteins which function as electron donors in a number of metabolic pathways in a broad range of organisms. In photosynthetic organisms, PETF, or ferredoxin 1 (FDX1), is the most studied ferredoxin due to its essential role in photosynthesis, where it transfers electrons from photosystem I to ferredoxin-NADP(+) oxidoreductase. However, PETF can also transfer electrons to a large number of other proteins. One important PETF electron acceptor found in green microalgae is the biologically and biotechnologically important [FeFe]-hydrogenase HYDA, which catalyses the production of molecular hydrogen (H2) from protons and electrons...
June 15, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28619534/cryptochrome-photoreceptors-in-green-algae-unexpected-versatility-of-mechanisms-and-functions
#16
Tilman Kottke, Sabine Oldemeyer, Sandra Wenzel, Yong Zou, Maria Mittag
Green algae have a highly complex and diverse set of cryptochrome photoreceptor candidates including members of the following subfamilies: plant, plant-like, animal-like, DASH and cryptochrome photolyase family 1 (CPF1). While some green algae encode most or all of them, others lack certain members. Here we present an overview about functional analyses of so far investigated cryptochrome photoreceptors from the green algae Chlamydomonas reinhardtii (plant and animal-like cryptochromes) and Ostreococcus tauri (CPF1) with regard to their biological significance and spectroscopic properties...
May 31, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28618080/electrocatalytic-mechanism-involving-michaelis-menten-kinetics-at-the-preparative-scale-theory-and-applicability-to-photocurrents-from-photosynthetic-algae-suspension-with-quinones
#17
Guillaume Longatte, Manon Guille-Collignon, Frédéric Lemaître
In the past years, many strategies were implemented to take benefits from oxygenic photosynthesis to harvest photosynthetic electrons and produce a significant photocurrent. Therefore, electrochemical tools were considered and globally relied on the electron transfer(s) between the photosynthetic chain and a collecting electrode. In this context, we recently reported the implementation of an electrochemical set-up at the preparative scale to produce photocurrents from a Chlamydomonas reinhardtii algae suspension with an appropriate mediator (2,6-DCBQ) and a carbon gauze as the working electrode...
June 15, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28615673/phototaxis-beyond-turning-persistent-accumulation-and-response-acclimation-of-the-microalga-chlamydomonas-reinhardtii
#18
Jorge Arrieta, Ana Barreira, Maurizio Chioccioli, Marco Polin, Idan Tuval
Phototaxis is an important reaction to light displayed by a wide range of motile microorganisms. Flagellated eukaryotic microalgae in particular, like the model organism Chlamydomonas reinhardtii, steer either towards or away from light by a rapid and precisely timed modulation of their flagellar activity. Cell steering, however, is only the beginning of a much longer process which ultimately allows cells to determine their light exposure history. This process is not well understood. Here we present a first quantitative study of the long timescale phototactic motility of Chlamydomonas at both single cell and population levels...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28612271/overexpression-of-dnaj-like-chaperone-enhances-carotenoid-synthesis-in-chlamydomonas-reinhardtii
#19
Tateki Morikawa, Yusuke Uraguchi, Shohei Sanda, Satoshi Nakagawa, Shigeki Sawayama
Production of functional carotenoids using microalgae may facilitate the commercialization of anti-aging nutritional supplements. The green alga Chlamydomonas reinhardtii uses a non-mevalonate (MEP) pathway for isopentenyl diphosphate (IPP) synthesis. Two enzymes thought to play important roles in this MEP pathway to IPP synthesis are 1-deoxy-D-xylulose 5-phosphate synthase (DXS) and reductase (DXR). DnaJ-like chaperone (Orange protein) is thought to support phytoene synthase, a key enzyme in plant carotenoid synthesis...
June 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28608341/isolation-and-characterization-of-argonaute-mutants-in-chlamydomonas
#20
Tomohito Yamasaki
Random insertional mutagenesis and subsequent reverse genetic screening allow the isolation of mutants of interest. Here I describe the protocol for generating a tag insertion line and subsequent PCR-based screening for ARGONAUTE mutants as an example of a reverse genetic screen for the unicellular green alga Chlamydomonas reinhardtii.
2017: Methods in Molecular Biology
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