keyword
https://read.qxmd.com/read/38533034/colorimetric-and-fluorescent-dual-biosensor-based-on-zirconium-and-preasodium-metal-organic-framework-zr-pr-mof-for-mirna-191-detection
#1
JOURNAL ARTICLE
Mahsa Dehnoei, Elnaz Ahmadi-Sangachin, Morteza Hosseini
MicroRNAs (miRNAs) are associated with certain types of cancer, tumor stages, and responses to treatment, thus efficient methods are required to identify them quickly and accurately. Abnormal expression of microRNA-191 (miR-191) has been linked to particular cancers and several other health conditions, such as diabetes and Alzheimer's disease. In this study, a new dual-biosensor based on the zirconium and preasodium-based metal-organic framework (Zr/Pr MOF) was developed for the rapid, ultrasensitive, and selective detection of miRNA-191...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38532845/acute-response-in-circulating-micrornas-following-a-single-bout-of-short-sprint-and-heavy-strength-training-in-well-trained-cyclists
#2
JOURNAL ARTICLE
Anita Ryningen, Kari Rostad, Elisabeth Ersvær, Gry Sjøholt, Gøran Paulsen, Hilde Gundersen, Morten Kristoffersen, Lise Bjørkhaug
Background: Heavy strength (HS) and short-sprint (SS) are commonly used training methods for competitive road cyclists, with the aim to improve the anaerobic power and short time cycling performance. Knowledge of how such training methods affects biochemical as well as molecular factors, are particularly important for determining individual recovery and long-term adaptations. The primary aim of the current study was to investigate the expression levels of small non-coding RNAs in response to HS and SS training in elite cyclists as potential biomarkers for individual optimal restitution time...
2024: Frontiers in Physiology
https://read.qxmd.com/read/38532190/exosomal-micrornas-in-parkinson-s-disease-insights-into-biomarker-potential-and-disease-pathology
#3
REVIEW
Deepthy Chandran, Syam Krishnan, Madhusoodanan Urulangodi, Srinivas Gopala
Parkinson's disease (PD) is a prevalent neurodegenerative condition primarily affecting the elderly population. Despite its high incidence in aged individuals, there are no reliable blood-based biomarkers for clinical diagnosis of PD and early screening of susceptible individuals. Recent studies have revealed the significance of exosomes in mediating cell-to-cell communications by transferring bioactive molecules, such as proteins, nucleic acids (including miRNAs), lipids, and metabolites, between cells. Due to their ability to carry diverse molecular cargo and their involvement in various physiological and pathological processes, exosomes have gained significant attention as potential disease biomarkers...
March 27, 2024: Neurological Sciences
https://read.qxmd.com/read/38528160/diagnostic-and-prognostic-significance-of-mir-486-5p-in-patients-who-underwent-minimally-invasive-surgery-for-lumbar-spinal-stenosis
#4
JOURNAL ARTICLE
Heqing Zhang, Dong Liu, Xiaoguang Fan
BACKGROUND: This study aimed to investigate the expression and clinical value of microRNA miR-486-5p in diagnosing lumbar spinal stenosis (LSS) patients and predicting the clinical outcomes after minimally invasive spinal surgery (MISS) in LSS patients, and the correlation of miR-486-5p with inflammatory responses in LSS patients. METHODS: This study included 52 LSS patients, 46 patients with lumbar intervertebral disk herniation (LDH) and 42 healthy controls. Reverse transcription quantitative PCR was used to detect miR-486-5p expression...
March 25, 2024: European Spine Journal
https://read.qxmd.com/read/38527406/electrochemiluminescence-biosensor-for-specific-detection-of-pancreatic-ductal-carcinoma-through-dual-targeting-of-muc1-and-mirna-196a
#5
JOURNAL ARTICLE
Yuxuan Chen, Zhuoxin Ye, Mo Ma, Jukun Yang, Ruiyan Liu, Yan Zhang, Pinyi Ma, Daqian Song
Pancreatic ductal adenocarcinoma (PDAC) poses significant diagnostic challenges due to its asymptomatic nature in its early stages, low specificity of conventional in vitro assays, and limited efficacy of surgical interventions. However, clinical specificity of the current serum biomarkers is suboptimal, leading to diagnostic inaccuracies and oversights. Therefore, this study introduced a novel dual-target electrochemiluminescence (ECL) biosensor to address these critical issues. The ECL biosensor synergistically employs the serum biomarker MUC1 and microRNA-196a to detect early-stage PDAC precisely...
March 22, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38525737/circulating-mirnas-as-novel-clinical-biomarkers-in-temporal-lobe-epilepsy
#6
REVIEW
Lorenza Guarnieri, Nicola Amodio, Francesca Bosco, Sara Carpi, Martina Tallarico, Luca Gallelli, Vincenzo Rania, Rita Citraro, Antonio Leo, Giovambattista De Sarro
Temporal lobe epilepsy (TLE) represents the most common form of refractory focal epilepsy. The identification of innovative clinical biomarkers capable of categorizing patients with TLE, allowing for improved treatment and outcomes, still represents an unmet need. Circulating microRNAs (c-miRNAs) are short non-coding RNAs detectable in body fluids, which play crucial roles in the regulation of gene expression. Their characteristics, including extracellular stability, detectability through non-invasive methods, and responsiveness to pathological changes and/or therapeutic interventions, make them promising candidate biomarkers in various disease settings...
March 17, 2024: Non-Coding RNA
https://read.qxmd.com/read/38523358/cancer-specific-risk-prediction-with-a-serum-microrna-signature
#7
JOURNAL ARTICLE
Janhavi R Raut, Megha Bhardwaj, Ben Schöttker, Bernd Holleczek, Petra Schrotz-King, Hermann Brenner
We recently derived and validated a serum-based microRNA risk score (miR-score) that predicted colorectal cancer (CRC) occurrence with very high accuracy within 14 years of follow-up in a population-based cohort study from Germany (ESTHER cohort). Here, we aimed to evaluate associations of the CRC-specific miR-score with the risk of developing other common cancers, including female breast cancer (BC), lung cancer (LC), and prostate cancer (PC), in the ESTHER cohort. MicroRNAs (miRNAs) were profiled by quantitative real-time PCR in serum samples collected at baseline from randomly selected incident cases of BC (n = 90), LC (n = 88), and PC (n = 93) and participants without diagnosis of CRC, LC, BC, or PC (controls, n = 181) until the end of the 17-year follow-up...
March 24, 2024: Cancer Science
https://read.qxmd.com/read/38521570/isothermal-strand-displacement-polymerase-reaction-isdpr-assisted-microchip-electrophoresis-for-highly-sensitive-detection-of-cancer-associated-micrornas
#8
JOURNAL ARTICLE
Jingyi Chen, Jingzi Zhang, Qihui Xie, Zhaohui Chu, Yuqi Lu, Fan Zhang, Qingjiang Wang
More and more studies have found that microRNAs (miRNAs) are markers of cancer, and detection of miRNAs is beneficial for early diagnosis and prognosis of cancer. In this paper, the isothermal strand displacement polymerase reaction (ISDPR), which is an enzyme-assisted nucleic acid amplification method, was studied to combine with microchip electrophoresis (MCE) for a simultaneously detection of two cancer related miRNAs named microRNA-21 (miR-21) and microRNA-221 (miR-221). In the ISDPR amplification, two different DNA hairpins (HPs) were specifically designed, so that miR-21 and miR-221 could respectively bind to HPs and started ISDPR amplification to generate two different products which were ultimately detected by MCE...
April 29, 2024: Analytica Chimica Acta
https://read.qxmd.com/read/38516774/mirna%C3%A2-21-promotes-the-progression-of-acute-liver-failure-via-the-klf6-autophagy-il%C3%A2-23-signaling-pathway
#9
JOURNAL ARTICLE
Suxia Bao, Weiyang Zheng, Rong Yan, Jie Xu
Acute liver failure (ALF) is a complex syndrome characterized by overactivation of innate immunity, and the recruitment and differentiation of immune cells at inflammatory sites. The present study aimed to explore the role of microRNA (miRNA/miR)‑21 and its potential mechanisms underlying inflammatory responses in ALF. Baseline serum miR‑21 was analyzed in patients with ALF and healthy controls. In addition, miR‑21 antagomir was injected via the tail vein into C57BL/6 mice, and lipopolysaccharide/D‑galactosamine (LPS/GalN) was injected into mice after 48 h...
May 2024: Molecular Medicine Reports
https://read.qxmd.com/read/38515724/profile-of-serum-micrornas-in-heart-failure-with-reduced-and-preserved-ejection-fraction-correlation-with-myocardial-remodeling
#10
JOURNAL ARTICLE
Layde Rosane Paim, Luis Miguel da Silva, Lígia M Antunes-Correa, Vinicius Citelli Ribeiro, Roberto Schreiber, Eduarda O Z Minin, Larissa C M Bueno, Elisangela C P Lopes, Renan Yamaguti, Andréa Coy-Canguçu, Sergio San Juan Dertkigil, Andrei Sposito, Jose Roberto Matos-Souza, Thiago Quinaglia, Tomas G Neilan, Licio A Velloso, Wilson Nadruz, Michael Jerosch-Herold, Otavio R Coelho-Filho
BACKGROUND AND AIMS: Cardiomyocyte hypertrophy and interstitial fibrosis are key components of myocardial remodeling in Heart Failure (HF) with preserved (HFpEF) or reduced ejection fraction (HFrEF). MicroRNAs (miRNAs) are non-coding, evolutionarily conserved RNA molecules that may offer novel insights into myocardial remodeling. This study aimed to characterize miRNA expression in HFpEF (LVEF <mml:math xmlns:mml="https://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mo>≥</mml:mo></mml:mrow></mml:math> 45%) and HFrEF (LVEF < 45%) and its association with myocardial remodeling...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38512134/correlation-study-on-the-prognostic-value-of-mir-21-and-s-100b-protein-levels-in-neonatal-hypoxic-ischemic-encephalopathy-undergoing-hypothermia-therapy
#11
JOURNAL ARTICLE
Yan Liu, Nuo-Xuan Liu
OBJECTIVE: To evaluate the variations in serum levels of microRNA-21 (miR-21) and S-100B protein in neonates with hypoxic-ischemic encephalopathy (HIE) after receiving hypothermia therapy and explore the correlation of these biomarkers with the neurodevelopmental prognosis of the infants. METHODS: This retrospective analysis included 90 neonatal HIE patients diagnosed and treated between January 2019 and December 2022. Real-time quantitative PCR and enzyme-linked immunosorbent assay (ELISA) methods were used to measure miR-21 and S-100B protein levels...
March 21, 2024: International Journal of Neuroscience
https://read.qxmd.com/read/38511059/systematic-review-and-meta-analysis-of-dysregulated-micrornas-derived-from-liquid-biopsies-as-biomarkers-for-amyotrophic-lateral-sclerosis
#12
JOURNAL ARTICLE
Hemerson Casado Gama, Mariana A Amorós, Mykaella Andrade de Araújo, Congzhou M Sha, Mirella P S Vieira, Rayssa G D Torres, Gabriela F Souza, Janaína A Junkes, Nikolay V Dokholyan, Daniel Leite Góes Gitaí, Marcelo Duzzioni
The discovery of disease-specific biomarkers, such as microRNAs (miRNAs), holds the potential to transform the landscape of Amyotrophic Lateral Sclerosis (ALS) by facilitating timely diagnosis, monitoring treatment response, and accelerating drug discovery. Such advancement could ultimately improve the quality of life and survival rates for ALS patients. Despite more than a decade of research, no miRNA biomarker candidate has been translated into clinical practice. We conducted a systematic review and meta-analysis to quantitatively synthesize data from original studies that analyzed miRNA expression from liquid biopsies via PCR and compared them to healthy controls...
June 2024: Non-Coding RNA Research
https://read.qxmd.com/read/38508125/highly-sensitive-mirna-21-detection-with-enzyme-free-cascade-amplification-biosensor
#13
JOURNAL ARTICLE
Qiuyan Huang, Kun Wang, Yuan Wang
In this study, we present an enzyme-free fluorescence biosensor for the highly sensitive detection of miRNA-21, a crucial biomarker in clinical diagnosis. Our innovative approach combines catalytic hairpin assembly (CHA) and entropy-driven amplification into a cascade amplification strategy. MicroRNA initiates the catalytic hairpin assembly reaction, liberating the trigger region needed for the entropy-driven amplification reaction. This triggers a series of strand displacement reactions, resulting in the separation of the fluorescence resonance energy transfer pair and an amplified fluorescence signal from FAM...
March 15, 2024: Talanta
https://read.qxmd.com/read/38505169/topical-delivery-of-microrna-125b-by-framework-nucleic-acids-for-psoriasis-treatment
#14
JOURNAL ARTICLE
Yunfeng Han, Long Xi, Fang Leng, Chenjie Xu, Ying Zheng
PURPOSE: Psoriasis is a chronic and recurrent inflammatory dermatitis characterized by T cell imbalance and abnormal keratinocyte proliferation. MicroRNAs (miRNAs) hold promise as therapeutic agents for this disease; however, their clinical application is hindered by poor stability and limited skin penetration. This study demonstrates the utilization of Framework Nucleic Acid (FNA) for the topical delivery of miRNAs in psoriasis treatment. METHODS: By utilizing miRNA-125b as the model drug, FNA-miR-125b was synthesized via self-assembly...
2024: International Journal of Nanomedicine
https://read.qxmd.com/read/38504194/circular-rna-hsa_circ_0005519-contributes-to-acute-kidney-injury-via-sponging-microrna-98-5p
#15
JOURNAL ARTICLE
Linsen Jiang, Manxin Huang, Jun Ge, Xuefeng Zhang, Ye Liu, Hang Liu, Xiaoming Liu, Lili Jiang
BACKGROUND: This study intends to explore the role and molecular mechanism of hsa_circ_0005519 in acute kidney injury (AKI). METHODS: We conducted reverse transcription-qPCR for human serum to determine levels of hsa_circ_0005519 in AKI patients and healthy controls. Hsa_circ_0005519 was inhibited for expression in HK-2 cells using specific siRNAs. A number of techniques, MTT and ELISA assays, were used to analyze the potential role of hsa_circ_0005519 in cell viability, oxidative stress, and inflammation of LPS-induced HK-2 cells...
March 19, 2024: BMC Nephrology
https://read.qxmd.com/read/38503014/ischemia-and-reperfusion-injured-liver-derived-exosomes-elicit-acute-lung-injury-through-mir-122-5p-regulated-alveolar-macrophage-polarization
#16
JOURNAL ARTICLE
Jingshu Lyu, Mingwei Sheng, Yingli Cao, Lili Jia, Chen Zhang, Yiqi Weng, Wenli Yu
Acute lung injury (ALI) is a common postoperative complication, particularly in pediatric patients after liver transplantation. Hepatic ischemia-reperfusion (HIR) increases the release of exosomes (IR-Exos) in peripheral circulation. However, the role of IR-Exos in the pathogenesis of ALI induced by HIR remains unclear. Here, we explored the role of exosomes derived from the HIR-injured liver in ALI development. Intravenous injection of IR-Exos caused lung inflammation in naive rats, whereas pretreatment with an inhibitor of exosomal secretion (GW4869) attenuated HIR-related lung injury...
March 18, 2024: International Immunopharmacology
https://read.qxmd.com/read/38499957/dynamics-of-serum-exosome-microrna-profile-altered-by-chemically-induced-estropause-and-rescued-by-estrogen-therapy-in-female-mice
#17
JOURNAL ARTICLE
Bianka Machado Zanini, Bianca Machado de Avila, Driele Neske Garcia, Jéssica Damé Hense, Gabriel Barreto Veiga, Mariana Machado Barreto, Sarah Ashiqueali, Jeffrey B Mason, Hariom Yadav, Michal Masternak, Augusto Schneider
The decline in the ovarian reserve leads to menopause and reduced serum estrogens. MicroRNAs are small non-coding RNAs, which can regulate gene expression and be secreted by cells and trafficked in serum via exosomes. Serum miRNAs regulate tissue function and disease development. Therefore, the aim of this study was to identify miRNA profiles in serum exosomes of mice induced to estropause and treated with 17β-estradiol (E2). Female mice were divided into three groups including control (CTL), injected with 4-Vinylcyclohexene diepoxide (VCD), and injected with VCD plus E2 (VCD + E2 )...
March 19, 2024: GeroScience
https://read.qxmd.com/read/38496750/prostate-derived-circulating-micrornas-add-prognostic-value-to-prostate-cancer-risk-calculators
#18
JOURNAL ARTICLE
Morgan L Zenner, Brenna Kirkpatrick, Trevor R Leonardo, Michael J Schlicht, Alejandra Cavazos Saldana, Candice Loitz, Klara Valyi-Nagy, Mark Maienschein-Cline, Peter H Gann, Michael Abern, Larisa Nonn
Prostate cancer is the second leading cause of malignancy-related deaths among American men. Active surveillance is a safe option for many men with less aggressive disease, yet definitively determining low-risk cancer is challenging with biopsy alone. Herein, we sought to identify prostate-derived microRNAs in patient sera and serum extracellular vesicles, and determine if those microRNAs improve upon the current clinical risk calculators for prostate cancer prognosis before and after biopsy. Prostate-derived intracellular and extracellular vesicle-contained microRNAs were identified by small RNA sequencing of prostate cancer patient explants and primary cells...
November 2023: J Extracell Biol
https://read.qxmd.com/read/38490646/intracranial-self-stimulation-reverses-impaired-spatial-learning-and-regulates-serum-microrna-levels-in-a-streptozotocin-induced-rat-model-of-alzheimer-disease
#19
JOURNAL ARTICLE
Andrea Riberas-Sánchez, Irene Puig-Parnau, Laia Vila-Solés, Soleil Garcia-Brito, Laura Aldavert-Vera, Pilar Segura-Torres, Gemma Huguet, Elisabet Kádár
BACKGROUND: The assessment of deep brain stimulation (DBS) as a therapeutic alternative for treating Alzheimer disease (AD) is ongoing. We aimed to determine the effects of intracranial self-stimulation at the medial forebrain bundle (MFB-ICSS) on spatial memory, neurodegeneration, and serum expression of microRNAs (miRNAs) in a rat model of sporadic AD created by injection of streptozotocin. We hypothesized that MFB-ICSS would reverse the behavioural effects of streptozotocin and modulate hippocampal neuronal density and serum levels of the miRNAs...
2024: Journal of Psychiatry & Neuroscience: JPN
https://read.qxmd.com/read/38487855/potential-diagnostic-value-of-serum-micrornas-for-19-cancer-types-a-meta-analysis-of-bioinformatics-data
#20
JOURNAL ARTICLE
ChauMyThanh Tran, PhongSon Dinh
Cancer is the second most common cause of mortality worldwide, accounting for almost 10 million deaths in 2020. These deaths were partly due to delayed diagnosis that led to deferred treatment. Therefore, new diagnostic methods are necessary to enhance the accuracy of noninvasive cancer detection. The present study developed a microRNA (miRNA)-based serum diagnostic marker for detecting a wide range of cancers. The study involved 61,019 serum samples from 19 different cancer types. A miRNA prediction model was established through bioinformatics analysis of serum samples from various cancer pathologies and qRT-PCR results from studies in PubMed aligned to the analysis criteria...
March 15, 2024: Journal of Biomolecular Structure & Dynamics
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