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Serum microRNA

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https://www.readbyqxmd.com/read/28644508/expression-of-serum-microrna-378-and-its-clinical-significance-in-renal-cell-carcinoma
#1
Lixin Shi, Lei Zhang, Chunyang Wang, Shengkun Sun, Xiyuan Cao, Xu Zhang
Studies have demonstrated that miRNA-378 is expressed in various malignant tumors. In the present study, we aimed to explore the expression of serum miRNA-378 and its clinical significance in renal cell carcinoma (RCC) patients. A total of 75 RCC patients, 63 renal cysts (RC) patients and 75 healthy controls were selected. The miRNA-378 level in RCC and RC groups was significantly higher than in healthy control group, with RCC group having the highest level. The miRNA-378 levels were significantly decreased within the same group after surgery...
April 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28643872/circulating-microrna-as-candidates-for-early-embryonic-viability-in-cattle
#2
K G Pohler, J A Green, L A Moley, S Gunewardena, W T Hung, R R Payton, X Hong, L K Christenson, T W Geary, M F Smith
Blood-borne extracellular vesicles (i.e. exosomes and microvesicles) carrying microRNAs (miRNAs) could make excellent biomarkers of disease and different physiologic states, including pregnancy status. We tested the hypothesis that circulating extracellular vesicle-derived miRNAs might differentiate the pregnancy status of cows that had maintained pregnancy to Day 30 from non-pregnant cows or from those that exhibited embryonic mortality between Days 17-30 of gestation. Cows were randomly assigned for artificial insemination with fertile semen (n= 36) or dead semen (n = 8; control group) on Day 0 (day of estrus)...
June 23, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28643818/serum-micrornas-are-altered-in-various-stages-of-chronic-kidney-disease-a-preliminary-study
#3
COMMENT
Benjamin Brigant, Valérie Metzinger-Le Meuth, Ziad A Massy, Nathalie McKay, Sophie Liabeuf, Marion Pelletier, Marion Sallée, Eléonore M'Baya-Moutoula, Pascale Paul, Tilman B Drueke, Stéphane Burtey, Laurent Metzinger
BACKGROUND: MicroRNAs (miRNAs) are innovative and informative blood-based biomarkers involved in numerous pathophysiological processes. In this study and based on our previous experimental data, we investigated miR-126, miR-143, miR-145, miR-155 and miR-223 as potential circulating biomarkers for the diagnosis and prognosis of patients with chronic kidney disease (CKD). The primary objective of this study was to assess the levels of miRNA expression at various stages of CKD. METHODS: RNA was extracted from serum, and RT-qPCR was performed for the five miRNAs and cel-miR-39 (internal control)...
February 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28642811/actinidia-chinensis-planch-improves-the-indices-of-antioxidant-and-anti-inflammation-status-of-type-2-diabetes-mellitus-by-activating-keap1-and-nrf2-via-the-upregulation-of-microrna-424
#4
Longfeng Sun, Xiaofei Li, Gang Li, Bing Dai, Wei Tan
The fruit juice of Actinidia chinensis Planch. has antioxidant and anti-inflammation properties on patients with type 2 diabetes mellitus (T2DM), but the molecular mechanism was unclear. The patients took the juice and the serum level of antioxidant miR-424, Kelch-like ECH-associated protein 1 (Keap1), erythroid-derived 2-like 2 (Nrf2), and biochemical indices were measured. The juice increased the levels of serum microRNA-424, Keap1, and Nrf2 and reduced the levels of interleukin-1 (IL-1) beta and IL-6 in T2DM patients...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28641313/evaluation-of-serum-microrna-biomarkers-for-gastric-cancer-based-on-blood-and-tissue-pools-profiling-the-importance-of-mir-21-and-mir-331
#5
Marek Sierzega, Marcin Kaczor, Piotr Kolodziejczyk, Jan Kulig, Marek Sanak, Piotr Richter
BACKGROUND: High stability and disease-specific disarrangements suggest that microRNA molecules (miRNAs) present in body fluids are ideally suited for diagnostic applications, including gastric cancer (GC). However, the actual source of circulating miRNA biomarkers in GC has not been adequately evaluated, particularly in the Western populations that have some distinct characteristics compared with Asian patients. METHODS: Twenty treatment-naive patients with GC along with 20 cancer-free controls were recruited...
June 22, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28640790/microrna-155-deficiency-in-kupffer-cells-ameliorates-liver-ischemia-reperfusion-injury-in-mice
#6
Yakun Li, Dongxia Ma, Zhimin Wang, Jun Yang
BACKGROUND: MicroRNA-155 (miR-155) is known to be involved in autoimmune diseases, inflammation, and transplantation. However, its role in a warm hepatic ischemia-reperfusion (IR) model has not been fully elucidated. METHODS: Partial hepatic IR was performed in wild-type and miR-155-deficient mice treated with or without GdCl3, and then the serum transaminase concentration and histology were analyzed. Kupffer cells (KCs) were isolated from the liver after IR, and immunohistochemistry was used to evaluate activation and polarization...
July 2017: Transplantation
https://www.readbyqxmd.com/read/28638795/potential-prognostic-biomarkers-in-pancreatic-juice-of-resectable-pancreatic-ductal-adenocarcinoma
#7
REVIEW
Shefali Agrawal
Despite potentially curative surgery pancreatic cancer has a dismal prognosis. Serum cancer antigen 19-9 (CA 19-9) correlates with tumor burden, resectability and survival in patients with pancreatic ductal adenocarcinoma. Identification of novel biomarkers may facilitate early diagnosis of pancreatic cancer and improve survival. Pancreatic juice is a rich source of cancer-specific proteins rendering it a promising tool for identifying biomarkers. Recent proteomic and microRNA expression analyses have identified several biomarkers of potential diagnostic and prognostic value...
June 10, 2017: World Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28638270/existence-of-a-strong-correlation-of-biomarkers-and-mirna-in-females-with-metabolic-syndrome-and-obesity-in-a-population-of-west-virginia
#8
Perrine Goguet-Rubio, Rebecca L Klug, Dana L Sharma, Krithika Srikanthan, Nitin Puri, Vishal H Lakhani, Alexandra Nichols, Kathleen M O'Hanlon, Nader G Abraham, Joseph I Shapiro, Komal Sodhi
Objectives: Metabolic syndrome causes complications like cardiovascular disease and type 2 diabetes mellitus (T2DM). As metabolic syndrome develops, altered levels of cytokines and microRNAs (miRNA) are measurable in the circulation. We aimed to construct a panel detecting abnormal levels of cytokines and miRNAs in patients at risk for metabolic syndrome. Methods: Participants included 54 patients from a Family Medicine Clinic at Marshall University School of Medicine, in groups of: Control, Obese, and Metabolic Syndrome (MetS)...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28638226/validation-of-a-serum-microrna-panel-as-biomarkers-for-early-diagnosis-of-hepatocellular-carcinoma-post-hepatitis-c-infection-in-egyptian-patients
#9
Moustafa Nouh Elemeery, Ahmed Noah Badr, Marwa Anwar Mohamed, Doaa Ahmed Ghareeb
AIM: To investigate the prospective importance of serum micro (mi)RNAs (miR-125b, miR-138b, miR-1269, miR-214-5p, miR-494, miR375 and miR-145) as early biomarkers for the diagnosis of hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC). METHODS: Two-hundred and fifty HCV4a patients, 224 HCV4a-HCC patients, and 84 healthy controls were enrolled in the study. Expression levels of miR214-5p, miR-125b, miR-1269 and miR-375 were quantified using quantitative real-time PCR...
June 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28637496/mir21-sensitized-b-lymphoma-cells-to-abt-199-via-icos-icosl-mediated-interaction-of-treg-cells-with-endothelial-cells
#10
Zhong Zheng, Peng-Peng Xu, Li Wang, Hui-Jin Zhao, Xiang-Qin Weng, Hui-Juan Zhong, Bin Qu, Jie Xiong, Yan Zhao, Xue-Feng Wang, Anne Janin, Wei-Li Zhao
BACKGROUND: MicroRNAs (miRs) are involved in tumor progression by regulating tumor cells and tumor microenvironment. MiR21 is overexpressed in diffuse large B-cell lymphoma (DLBCL) and its biological impact on tumor microenvironment remains unclear. METHODS: MiR21 was assessed by quantitative RT-PCR in patients with newly diagnosed DLBCL. The mechanism of action of miR21 on lymphoma progression and tumor angiogenesis was examined in vitro in B-lymphoma cell lines and in vivo in a murine xenograft model...
June 21, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28637221/digital-direct-detection-of-micrornas-using-single-molecule-arrays
#11
Limor Cohen, Mark R Hartman, Aaron Amardey-Wellington, David R Walt
MicroRNAs (miRNAs) are involved in many biological pathways, and detecting miRNAs accurately is critical for diagnosing a variety of diseases including cancer. However, most current methods for miRNA detection require lengthy sample preparation and amplification steps that can bias the results. In addition, lack of specificity and reproducibility give rise to various challenges in detection of circulating miRNAs in biological samples. In this work, we applied the Single Molecule Array (Simoa) technique to develop an ultra-sensitive sandwich assay for direct detection of multiple miRNAs without pre-amplification...
June 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28636562/exosome-encapsulated-micrornas-as-circulating-biomarkers-for-colorectal-cancer
#12
Shushan Yan, Bing Han, Shunyuan Gao, Xiaochen Wang, Zengfang Wang, Fakai Wang, Jianjun Zhang, Donghua Xu, Beicheng Sun
Currently available studies have suggested that a number of exosome-encapsulated microRNAs (miRNAs) are recognized as stable biomarkers for cancers. However, little is known about the effect of exosomal miRNAs on colorectal cancer (CRC). The aim of study is to identify specific miRNAs in serum exosomes, which may serve as potential diagnostic and prognostic biomarkers and therapeutic targets for CRC. Microarray analyses of miRNAs in serum exosomes from 3 primary CRC patients and 3 healthy controls were performed...
June 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28632432/serum-micrornas-as-early-indicators-for-estimation-of-exposure-degree-in-response-to-ionizing-irradiation
#13
Wenjun Wei, Jinpeng He, Jufang Wang, Nan Ding, Bing Wang, Sulan Lin, Xurui Zhang, Junrui Hua, He Li, Burong Hu
Exposure to ionizing radiation from nuclear devices, spaceflights or terrorist attacks represents a major threat to human health and public security. After a radiological incident, noninvasive biomarkers that can facilitate rapid assessment of exposure risk in the early stages are urgently needed for optimal medical treatment. Serum microRNAs (miRNAs) are ideal biomarkers because they are stable in response to environmental changes, they are common among different species and are easily collected. Here, we performed miRNA PCR arrays to analyze miRNA expression profiles at 24 h postirradiation...
June 20, 2017: Radiation Research
https://www.readbyqxmd.com/read/28628844/serum-micrornas-in-buffalo-cows-potential-biomarkers-of-pregnancy
#14
Gabriella Guelfi, Valentina Stefanetti, Silvio De Luca, Andrea Giontella, Vittoria Lucia Barile, Olimpia Barbato
MiRNAs (microRNA) constitute a large family of single-stranded, non-coding small RNAs. Although the functions and target genes of most miRNAs are still unknown, it has been well established that they are involved in embryogenesis, organogenesis and neonatal birth. In recent decades, interest in buffalo breeding has largely increased worldwide thus focusing the attention on this species as a dairy purpose animal. Problems related to long calving interval, late puberty and seasonal anestrus hamper reproductive efficiency in this species...
June 4, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28628652/microrna-433-3p-promotes-osteoblast-differentiation-through-targeting-dkk1-expression
#15
Xiaolin Tang, Jiantao Lin, Guanhai Wang, Jianlin Lu
Dickkopf-1 (DKK1) is a powerful antagonist of canonical WNT signaling pathway, and is regarded as a biomarker for osteoporosis. Its expression is highly correlated with bone mass and osteoblasts maturation. In this study, mouse primary bone marrow cells and osteoblast cell lines were used. Luciferase reporter assay and western blotting methods were employed to validate if miRNA-433-3p epigenetically regulated DKK1 translation. Rat bone marrow derived osteoblasts were infected with lentivirus vector in which miR-433-3p was constructed...
2017: PloS One
https://www.readbyqxmd.com/read/28627619/expression-of-dicer-in-rheumatoid-arthritis-is-associated-with-disease-activity-and-balances-the-production-of-tnf%C3%A2-%C3%AE
#16
Shujun Wang, Min Yuan, Lijun Song, Xiuying Zhang, Qin Geng, Hongju Zhang, Xingfu Li
Gene expression can be altered through RNA interference (RNAi), including microRNA (miRNA) or small interfering RNA. Alterations of miRNAs in rheumatoid arthritis (RA) have been reported, however, the components of the RNAi machinery in RA remain to be fully elucidated. The aim of the present study was to detect the levels of Dicer, Argonaute2 and Drosha, components of the RNAi machinery, in the peripheral blood mononuclear cells of patients with RAusingreverse transcription‑quantitative polymerase chain reaction, and to compare the results with disease activity and clinical features...
June 9, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28626163/lower-serum-levels-of-mir-29c-3p-and-mir-19b-3p-as-biomarkers-for-alzheimer-s-disease
#17
Yuquan Wu, Juan Xu, Jing Xu, Jun Cheng, Demin Jiao, Chun Zhou, Yi Dai, Qingyong Chen
MicroRNAs (miRNAs) are short noncoding RNA that participate in posttranscriptional gene regulation. However, little is understood about the roles of miRNAs in Alzheimer's disease (AD). In this study, we used next-generation sequencing on RNA extracted from the serum samples of 20 AD patients and 20 controls, yielding a total of 72 miRNAs with significantly changed expression levels. Among these candidates, we selected 9 miRNAs with most significant alteration in disease, and validated their expression levels using RT-qPCR analysis on serum samples from 45 AD patients and 40 control subjects...
2017: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28620131/exosomes-containing-differential-expression-of-microrna-and-mrna-in-osteosarcoma-that-can-predict-response-to-chemotherapy
#18
Ji-Feng Xu, Ya-Ping Wang, Shui-Jun Zhang, Yu Chen, Hai-Feng Gu, Xiao-Fan Dou, Bing Xia, Qing Bi, Shun-Wu Fan
A major challenge in osteosarcoma (OS) is the selection of the most effective chemotherapeutic agents for individual patients, while the administration of ineffective chemotherapy increases mortality and decreases quality of life in patients. This emphasizes the need to evaluate every patient's probability of responding to each chemotherapeutic agent. We developed a profiling strategy for serum exosomal microRNAs and mRNAs in OS patients with differential chemotherapeutic responses. Twelve miRNAs were up regulated and 18 miRNAs were under regulated significantly in OS patient with poor chemotherapeutic response when compared with those in good chemotherapeutic response (p<0...
June 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28618945/the-expression-profiling-of-serum-mir-92a-mir-375-and-mir-760-in-colorectal-cancer-an-egyptian-study
#19
Ahmed Elshafei, Olfat Shaker, Ossama Abd El-Motaal, Tarek Salman
Dysregulation in microRNA expression is a common feature in colorectal cancer. Due to the inconsistent results regarding serum miR-92a expression pattern and the insufficient studies on serum miR-375 and miR-760, we aimed in this study to investigate their expression profile and diagnostic and prognostic power in Egyptian colorectal cancer patients. The expression profile of miR-92a, miR-375, and miR-760 was determined in the sera of 64 colorectal cancer patients using quantitative real-time reverse transcription polymerase chain reaction in comparison to 27 healthy control subjects...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28617546/down-expression-of-mir-373-predicts-poor-prognosis-of-glioma-and-could-be-a-potential-therapeutic-target
#20
S-Y Jing, S-Q Jing, L-L Liu, L-F Xu, F Zhang, J-L Gao
OBJECTIVE: MicroRNAs (miRNAs) are epigenetic regulators of gene expression, and their deregulation plays an important role in human cancer, including glioma. The main objective of this work was to investigate the expression level of miR-373 and its clinical significance in glioma. PATIENTS AND METHODS: The expression levels of miR-373 in glioma tissues and non-neoplastic brain tissues were measured by the qRT-PCR assay. Patients were divided into two groups based on the median miR-373 expression...
May 2017: European Review for Medical and Pharmacological Sciences
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