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Serum microRNA

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https://www.readbyqxmd.com/read/28317854/sets-of-serum-exosomal-micrornas-as-candidate-diagnostic-biomarkers-for-kawasaki-disease
#1
Hong-Ling Jia, Chao-Wu Liu, Li Zhang, Wei-Jun Xu, Xue-Juan Gao, Jun Bai, Yu-Fen Xu, Ming-Guo Xu, Gong Zhang
Although Kawasaki disease is the main cause of acquired heart disease in children, no diagnostic biomarkers are available. We aimed to identify candidate biomarkers for diagnosing Kawasaki disease using serum exosomal microRNAs (miRNAs). Using frozen serum samples from a biobank, high-throughput microarray technologies, two-stage real-time quantitative PCR, and a self-referencing strategy for data normalization, we narrowed down the list of biomarker candidates to a set of 4 miRNAs. We further validated the diagnostic capabilities of the identified miRNAs (namely, CT(miR-1246)-CT(miR-4436b-5p) and CT(miR-197-3p)-CT(miR-671-5p)) in 79 samples from two hospitals...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28299339/cytoplasmic-localization-of-wt1-and-decrease-of-mirna-16-1-in-nephrotic-syndrome
#2
Pablo Zapata-Benavides, Mariela Arellano-Rodríguez, Juan José Bollain-Y-Goytia, Moisés Armides Franco-Molina, Gloria Azucena Rangel-Ochoa, Esperanza Avalos-Díaz, Rafael Herrera-Esparza, Cristina Rodríguez-Padilla
Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) expression in podocytes. Downregulation of WT1 has been shown to be affected by certain microRNAs (miRNAs). Twenty-one patients with idiopathic NS (68.75% were SSNS and 31.25% SRNS) and 10 healthy controls were enrolled in the study...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28298326/let-7-and-microrna-148-regulate-parathyroid-hormone-levels-in-secondary-hyperparathyroidism
#3
Vitali Shilo, Irit Mor-Yosef Levi, Roy Abel, Aleksandra Mihailović, Gilad Wasserman, Tally Naveh-Many, Iddo Z Ben-Dov
Secondary hyperparathyroidism commonly complicates CKD and associates with morbidity and mortality. We profiled microRNA (miRNA) in parathyroid glands from experimental hyperparathyroidism models and patients receiving dialysis and studied the function of specific miRNAs. miRNA deep-sequencing showed that human and rodent parathyroids share similar profiles. Parathyroids from uremic and normal rats segregated on the basis of their miRNA expression profiles, and a similar finding was observed in humans. We identified parathyroid miRNAs that were dysregulated in experimental hyperparathyroidism, including miR-29, miR-21, miR-148, miR-30, and miR-141 (upregulated); and miR-10, miR-125, and miR-25 (downregulated)...
March 15, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28292471/elevated-microrna-520g-in-pre-eclampsia-inhibits-migration-and-invasion-of-trophoblasts
#4
Liansheng Jiang, Anxiong Long, Longyi Tan, Mao Hong, Jingjing Wu, Leiming Cai, Qian Li
INTRODUCTION: Pre-eclampsia (PE) is a common and severe obstetric complication. MicroRNAs (miRs) have emerged as molecules that are associated with the disease. METHODS: Quantitative reverse transcription PCR (RT-qPCR) was used for serum miR-520g characterization from 19 severe pre-eclamptic and 19 normal pregnancies. In situ hybridation was adopted to localize microRNA-520g (miR-520g). Migration and invasion of HTR-8/SVneo cells were evaluated after miR-520g mimic treatment with transwell system...
March 2017: Placenta
https://www.readbyqxmd.com/read/28288169/the-mir-200-family-is-increased-in-dysplastic-lesions-in-ulcerative-colitis-patients
#5
Amy Lewis, Carla Felice, Tomoko Kumagai, Cecilia Lai, Kriti Singh, Rosemary R Jeffery, Roger Feakins, Eleni Giannoulatou, Alessandro Armuzzi, Noor Jawad, James O Lindsay, Andrew Silver
BACKGROUND: Colorectal cancer (CRC) is a life-threatening complication of ulcerative colitis (UC), and patients are routinely screened for the development of precancerous lesions (dysplasia). However, rates of CRC development in patients with confirmed low-grade dysplasia vary widely between studies, suggesting a large degree of heterogeneity between these lesions that is not detectable macroscopically. A better understanding of the underlying molecular changes that occur in dysplasia will help to identify lesions at higher risk of malignancy...
2017: PloS One
https://www.readbyqxmd.com/read/28288140/microrna-377-suppresses-initiation-and-progression-of-esophageal-cancer-by-inhibiting-cd133-and-vegf
#6
B Li, W W Xu, L Han, K T Chan, S W Tsao, N P Y Lee, S Law, L Y Xu, E M Li, K W Chan, Y R Qin, X Y Guan, Q Y He, A L M Cheung
Esophageal cancer is one of the most lethal cancers worldwide with poor survival and limited therapeutic options. The discovery of microRNAs created a new milestone in cancer research. miR-377 is located in chromosome region 14q32, which is frequently deleted in esophageal squamous cell carcinoma (ESCC), but the biological functions, clinical significance and therapeutic implication of miR-377 in ESCC are largely unknown. In this study, we found that miR-377 expression was significantly downregulated in tumor tissue and serum of patients with ESCC...
March 13, 2017: Oncogene
https://www.readbyqxmd.com/read/28285201/simple-sensitive-and-label-free-electrochemical-detection-of-micrornas-based-on-the-in-situ-formation-of-silver-nanoparticles-aggregates-for-signal-amplification
#7
Leilei Liu, Yong Chang, Ning Xia, Peizhen Peng, Liping Zhang, Mengsha Jiang, Jiebin Zhang, Lin Liu
This work presented a simple, sensitive and label-free electrochemical method for the detection of microRNAs (miRNAs). It is based on the boronate ester covalent interaction between 4-mercaptophenylboronic acid (MPBA) and cis-diol at the 3'-terminal of miRNAs and the MPBA-induced in situ formation of citrate-capped silver nanoparticles (AgNPs) aggregates as labels on the electrode surface. In this design, MPBA acted as the cross-linker of AgNPs assembly. Specifically, the thiolated hairpin-like DNA probe was assembled onto the gold nanoparticles (nano-Au) modified electrode surface through the Ag-S interaction...
February 24, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28284697/mir-27a-controls-triacylglycerol-synthesis-in-bovine-mammary-epithelial-cells-by-targeting-peroxisome-proliferator-activated-receptor-gamma
#8
K Q Tang, Y N Wang, L S Zan, W C Yang
Growing evidence has revealed that microRNA are central elements in milk fat synthesis in mammary epithelial cells. A negative regulator of adipocyte fat synthesis, miR-27a has been reported to be involved in the regulation of milk fat synthesis in goat mammary epithelial cells; however, the regulatory role of miR-27a in bovine milk fat synthesis remains unclear. In the present study, primary bovine mammary epithelial cells (BMEC) were harvested from mid-lactation cows and cultured in Dulbecco's modified Eagle's medium/F-12 medium with 10% fetal bovine serum, 5 μg/mL of insulin, 1 μg/mL of hydrocortisone, 2 μg/mL of prolactin, 1 μg/mL of progesterone, 100 U/mL of penicillin, and 100 μg/mL of streptomycin...
March 8, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28283537/increased-osteoclastogenesis-in-patients-with-vertebral-fractures-following-discontinuation-of-denosumab-treatment
#9
Athanasios D Anastasilakis, Maria P Yavropoulou, Polyzois Makras, Grigorios T Sakellariou, Foteini Papadopoulou, Spyridon Gerou, Socrates Papapoulos
OBJECTIVE: To test the hypothesis that rebound of bone remodeling is responsible for clinical vertebral fractures reported in a few patients with osteoporosis after cessation of denosumab treatment. DESIGN: In this case-control study we compared clinical and biochemical characteristics of postmenopausal women with clinical vertebral fractures 8 to 16 months after the last injection of denosumab (Dmab/Fx+, n=5) with those of treatment-naïve women with such fractures (Fx+, n=5)...
March 10, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28279125/micrornas-as-novel-biomarkers-for-the-diagnosis-and-prognosis-of-mild-and-severe-traumatic-brain-injury
#10
Valentina Di Pietro, Marco Ragusa, David James Davies, Zhangjie Su, Jon Hazeldine, Giacomo Lazzarino, Lisa J Hill, Nicholas Crombie, Mark Foster, Michele Purrello, A Logan, Antonio Belli
Traumatic brain injury (TBI) is the leading cause of death and disability under the age of 45 years in Western countries. Despite many studies, no reliable biomarkers have been found to assess TBI severity and predict recovery. MicroRNA (miRNA) profiling has become widely used to identify biomarkers and therapeutic targets. The expression of 754 miRNAs was analysed in serum of 5 mild TBI (mTBI) with extra-cranial injury (EC) patients, 5 severe TBI (sTBI) with EC patients and 5 healthy volunteers (HV) at 1 day and 15 days post injury, by using the TaqMan® Array Human MicroRNA A+B Cards...
March 9, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/28278511/mechanism-of-microrna-146a-notch2-signaling-regulating-il-6-in-graves-ophthalmopathy
#11
Ning Wang, Feng-E Chen, Zi-Wen Long
BACKGROUND/AIMS: We intended to investigate the significance of microRNA-146a, Notch2 and IL-6 on Graves ophthalmopathy (GO) and the relationships among them. METHODS: About 27 GO patients were incorporated in this study, including 13 patients with inactive GO and14 patients with active GO. Another 15 patients who had previously received strabismus orthopedics or ophthalmectomy due to trauma were selected as the control population. QRT-PCR assay was used to detect microRNA-146a and Notch2 expression levels in plasma...
March 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28277542/microrna-27a-promotes-podocyte-injury-via-ppar%C3%AE-mediated-%C3%AE-catenin-activation-in-diabetic-nephropathy
#12
Zhanmei Zhou, Jiao Wan, Xiaoyan Hou, Jian Geng, Xiao Li, Xiaoyan Bai
Podocyte injury has a pivotal role in the pathogenesis of diabetic nephropathy (DN). MicroRNA-27a (miR-27a), peroxisome proliferator-activated receptor γ (PPARγ) and β-catenin pathways have been involved in the pathogenesis of DN. Herein, we asked whether miR-27a mediates podocyte injury through PPARγ/β-catenin signaling in DN. The functional relevance of miR-27a, PPARγ and β-catenin were investigated in cultured podocytes and glomeruli of diabetic rats and patients using in vitro and in vivo approaches...
March 9, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28275578/micrornas-for-the-prediction-of-early-response-to-sorafenib-treatment-in-human-hepatocellular-carcinoma
#13
Naoshi Nishida, Tadaaki Arizumi, Satoru Hagiwara, Hiroshi Ida, Toshiharu Sakurai, Masatoshi Kudo
BACKGROUND: Several studies suggest the role of circulating microRNAs (miRNAs) as biomarkers of hepatocellular carcinoma (HCC). However, the serum miRNA profile associated with the response to sorafenib remains to be elucidated. The aim of this study was to clarify the specific miRNAs in serum that could predict the early response of HCC to sorafenib treatment. SUMMARY: Analyzing the sera from 16 HCC patients, we selected five miRNAs that showed differences in serum levels between patients with and without tumor responses among 179 known secretory miRNAs by using locked nucleic acid probe-based quantitative PCR...
February 2017: Liver Cancer
https://www.readbyqxmd.com/read/28272709/expression-of-microrna-122-and-microrna-22-in-hbv-related-liver-cancer-and-the-correlation-with-clinical-features
#14
D-D Qiao, J Yang, X-F Lei, G-L Mi, S-L Li, K Li, C-Q Xu, H-L Yang
OBJECTIVE: MicroRNAs (miR) participate in cell proliferation, apoptosis and transformation, as they can regulate gene expression and intracellular signal transduction for various physiological processes. MiR-122 and miR-22 are known to be related with occurrence and progression of hepatitis B virus (HBV)-related hepatocellular cancer (HCC). This study recruited HBV-related HCC patients, whose expression levels of miR-122 and miR-22 were determined to analyze the correlation with clinical and pathological indexes...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28271451/the-utility-of-biomarkers-in-osteoporosis-management
#15
REVIEW
Patrick Garnero
The measurement of bone turnover markers is useful for the clinical investigation of patients with osteoporosis. Among the available biochemical markers, the measurements of serum procollagen type I N-terminal propeptide (PINP) and the crosslinked C-terminal telopeptide (serum CTX) have been recommended as reference markers of bone formation and bone resorption, respectively. The important sources of preanalytical and analytical variability have been identified for both markers, and precise measurement can now be obtained...
March 7, 2017: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/28270244/expression-profiling-of-circulating-mirnas-in-mouse-serum-in-response-to-echinococcus-multilocularis-infection
#16
Xiaola Guo, Yadong Zheng
Echinococcus multilocularis is a most pathogenic zoonotic tapeworm that causes devastating echinococcosis in both humans and animals. Circulating microRNAs (miRNAs) are stably existed in the serum/plasma of mammalian hosts during helminthic infection. In this study, we compared the host-circulating miRNA expression in the sera from the E. multilocularis-infected and uninfected mice. A total of 58 host-origin serum miRNAs were differentially expressed (2 ⩾ fold change, P < 0·05), of which 21 were upregulated and 37 were significantly downregulated...
March 8, 2017: Parasitology
https://www.readbyqxmd.com/read/28267418/microrna-125b-5p-mediates-post-transcriptional-regulation-of-hepatitis-b-virus-replication-via-the-lin28b-let-7-axis
#17
Wanyu Deng, Xiaoyong Zhang, Zhiyong Ma, Yong Lin, Mengji Lu
MicroRNAs (miRNAs) are able to modulate hepatitis B virus (HBV) replication and play an important role in the pathogenesis of HBV infection. Recently, we have identified that serum miR-125b-5p level correlated with HBV DNA levels and liver necroinflammation. In the present study, we addressed how miR-125b-5p regulated HBV replication at the different steps, inclduing viral transcription, assembly, and virion production and investigated the underlying mechanisms. We found that miR-125b-5p overexpression increased HBV replication without altering HBV transcription...
March 7, 2017: RNA Biology
https://www.readbyqxmd.com/read/28265343/high-fat-diet-dysregulates-microrna-17-5p-and-triggers-retinal-inflammation-role-of-endoplasmic-reticulum-stress
#18
Maha Coucha, Islam N Mohamed, Sally L Elshaer, Osinakachuk Mbata, Megan L Bartasis, Azza B El-Remessy
AIM: To elucidate how high diet-induced endoplasmic reticulum-stress upregulates thioredoxin interacting protein expression in Müller cells leading to retinal inflammation. METHODS: Male C57Bl/J mice were fed either normal diet or 60% high fat diet for 4-8 wk. During the 4 wk study, mice received phenyl-butyric acid (PBA); endoplasmic reticulum-stress inhibitor; for 2 wk. Insulin resistance was assessed by oral glucose tolerance. Effects of palmitate-bovine serum albumin (BSA) (400 μmol/L) were examined in retinal Müller glial cell line and primary Müller cells isolated from wild type and thioredoxin interacting protein knock-out mice...
February 15, 2017: World Journal of Diabetes
https://www.readbyqxmd.com/read/28264458/maternal-low-protein-diet-modulates-glucose-metabolism-and-hepatic-micrornas-expression-in-the-early-life-of-offspring-%C3%A2
#19
Jia Zheng, Xinhua Xiao, Qian Zhang, Tong Wang, Miao Yu, Jianping Xu
Emerging studies revealed that maternal protein restriction was associated with increased risk of type 2 diabetes mellitus in adulthood. However, the mechanisms of its effects on offspring, especially during early life of offspring, are poorly understood. Here, it is hypothesized that impaired metabolic health in offspring from maternal low-protein diet (LPD) is associated with perturbed miRNAs expression in offspring as early as the weaning age. We examined the metabolic effects on the C57BL/6J mice male offspring at weaning from dams fed with LPD or normal chow diet (NCD) throughout pregnancy and lactation...
February 27, 2017: Nutrients
https://www.readbyqxmd.com/read/28262692/mir-139-5p-as-a-novel-serum-biomarker-for-recurrence-and-metastasis-in-colorectal-cancer
#20
Jinsei Miyoshi, Shusuke Toden, Kazuhiro Yoshida, Yuji Toiyama, Steven R Alberts, Masato Kusunoki, Frank A Sinicrope, Ajay Goel
Approximately 30-50% of colorectal cancer (CRC) patients who undergo curative resection subsequently experience tumor recurrence or metastasis. Although microRNAs (miRNAs) are a class of small noncoding RNAs frequently deregulated in various human malignancies, it remains unknown if these can help predict recurrence and metastasis in CRC patients. MiRNAs were initially screened using miRNA-microarray and miRNA-seq datasets with or without recurrence. Candidate miRNAs were then tested in two independent cohorts of 111 stage II/III and 139 stage I-III CRC patients, as well as serum samples and matched primary and metastatic liver tissues...
March 6, 2017: Scientific Reports
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