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Serum microRNA

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https://www.readbyqxmd.com/read/28545106/serum-microrna-signatures-as-liquid-biopsies-for-interrogating-hepatotoxic-mechanisms-and-liver-pathogenesis-in-human
#1
Julian Krauskopf, Theo M de Kok, Shelli J Schomaker, Mark Gosink, Deborah A Burt, Patricia Chandler, Roscoe L Warner, Kent J Johnson, Florian Caiment, Jos C Kleinjans, Jiri Aubrecht
MicroRNAs (miRNAs) released into the peripheral circulation upon cellular injury have shown a promise as a new class of tissue-specific biomarkers. We were first to demonstrate that next-generation sequencing analysis of serum from human subjects with acetaminophen-induced liver injury revealed a specific signature of circulating miRNAs. We consequently hypothesized that different types of hepatic liver impairments might feature distinct signatures of circulating miRNAs and that this approach might be useful as minimally invasive diagnostic "liquid biopsies" enabling the interrogation of underlying molecular mechanisms of injury in distant tissues...
2017: PloS One
https://www.readbyqxmd.com/read/28540143/association-between-coronary-artery-disease-and-microrna-literature-review-and-clinical-perspective
#2
Rehan Malik, Raja S Mushtaque, Usman A Siddiqui, Adnan Younus, Muhammad A Aziz, Choudhry Humayun, Kanaan Mansoor, Muhammad A Latif, Salman Waheed, Salman Assad, Idrees Khan, Syed M Bukhari, Daniel DelCampo, Ali Adus, Swetha Gannarapu
BACKGROUND: Until recently, circulating micro-RNAs (miRNAs) have attracted major interest as novel biomarkers for the early diagnosis of coronary artery disease (CAD). This review article summarizes the available evidence on the correlation of micro-RNAs with both the clinical and subclinical coronary artery disease and highlights the necessity for exploring miRNAs as a potential diagnostic and prognostic biomarker of early CAD in an adult population. METHODS: A systematic literature analysis and retrieval online systems Public/Publisher MEDLINE/ Excerpta Medica Database /Medical Literature Analysis and Retrieval System Online,(PUBMED/EMBASE/MEDLINE) search were conducted for relevant information...
April 23, 2017: Curēus
https://www.readbyqxmd.com/read/28537679/mir-15a-expression-analysis-in-non-small-cell-lung-cancer-a549-cells-under-local-hypoxia-microenvironment
#3
X-G Jing, T-F Chen, C Huang, H Wang, L An, Z Cheng, G-J Zhang
OBJECTIVE: Lung cancer is a common tumor in the clinic. Hypoxia is an important biological characteristic in the solid malignant tumor. MiRNA participates in cell proliferation, differentiation, and apoptosis. This study tested hypoxia-inducible factor 1-α (HIF-1α) in lung cancer patients and analyzed the microRNA-15a (miR-15a) expression in A549 cells under different local hypoxia microenvironments. PATIENTS AND METHODS: A total of 40 non-small cell lung cancer (NSCLC) patients in First Affiliated Hospital of Zhengzhou University between Jan 2015 and Jan 2016 were involved in this study...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28537131/acupuncture-ameliorates-postoperative-ileus-via-il-6-mir-19a-kit-axis-to-protect-interstitial-cells-of-cajal
#4
Jingjing Deng, Shu Yang, Qing Yuan, Yuzhong Chen, Dandan Li, Haimei Sun, Xinghua Tan, Fuchun Zhang, Deshan Zhou
Acupuncture is a therapy effective in treating postoperative ileus (POI); its underlying mechanisms remain unclear. MicroRNAs (miRNAs) participate in inflammation and injury to the interstitial cells of Cajal (ICCs), both of which are considered to be contributors to POI. C-kit, encoding KIT, a specific marker of ICCs, is predicted to be targeted by miR-19a, an inflammation-related miRNA. Therefore, we investigated a possible link between inflammation, miR-19a, and ICCs in POI, as well as the mechanism by which these factors are affected by acupuncture...
May 24, 2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28529949/microrna-expression-varies-according-to-glucose-tolerance-measurement-platform-and-biological-source
#5
S Dias, S Hemmings, C Muller, J Louw, C Pheiffer
Dysregulated microRNA (miRNA) expression is observed during type 2 diabetes (T2D), although the consistency of miRNA expression across measurement platform and biological source is uncertain. Here we report miRNA profiling in the whole blood and serum of South African women with different levels of glucose tolerance, using next generation sequencing (NGS) and quantitative real time PCR (qRT-PCR). Whole blood-derived miRNAs from women with newly diagnosed T2D (n = 4), impaired glucose tolerance (IGT) (n = 4), and normal glucose tolerance (NGT) (n = 4) were subjected to NGS, whereafter transcript levels of selected miRNAs were quantified in the whole blood and serum of these women using qRT-PCR...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28523996/micro-rnas-exercise-and-cellular-plasticity-in-humans-the-impact-of-dietary-factors-and-hypoxia
#6
Timothée Watier, Anthony Mj Sanchez
Physical exercise is a stress that modulates a plethora of cellular mechanisms to promote morphological and metabolic adaptations. Substantial efforts have been made to better understand the modulation of pathways involved in cell plasticity during exercise, but human explorations may be limited because invasive procedures are often required. In recent years, minimally invasive methods for the analysis of microRNAs (miRNAs) modulation in response to exercise have been developed. miRNAs are small molecules that belong to a family of non-coding RNAs...
May 19, 2017: MicroRNA
https://www.readbyqxmd.com/read/28523463/immature-exosomes-derived-from-microrna-146a-overexpressing-dendritic-cells-act-as-antigen-specific-therapy-for-myasthenia-gravis
#7
Weifan Yin, Song Ouyang, Zhaohui Luo, Qiuming Zeng, Bo Hu, Liqun Xu, Yuan Li, Bo Xiao, Huan Yang
Myasthenia gravis (MG) is a neurological autoimmune disease characterized by fluctuating weakness of certain voluntary muscles. Current treatments for MG are largely directed at suppressing the whole immune system by using immunosuppressants or glucocorticoids and often cause several side effects. The ideal therapeutic methods for MG should suppress aberrant immunoactivation specifically, while retaining normal function of the immune system. In this study, we first produced exosomes from microRNA-146a overexpressing dendritic cells (DCs)...
May 18, 2017: Inflammation
https://www.readbyqxmd.com/read/28522697/circulating-microrna-125b-predicts-the-presence-and-progression-of-uremic-vascular-calcification
#8
Chia-Ter Chao, You-Pi Liu, Sheng-Fang Su, Hsiang-Yuan Yeh, Hsuan-Yu Chen, Pei-Jung Lee, Wan-Jiun Chen, Yee-Ming Lee, Jenq-Wen Huang, Chih-Kang Chiang, Kuan-Yu Hung, Huei-Wen Chen
OBJECTIVE: Vascular calcification (VC) is a major cause of mortality in patients with end-stage renal diseases. Biomarkers to predict the progression of VC early are in urgent demand. APPROACH AND RESULTS: We identified circulating, cell-free microRNAs as potential biomarkers using in vitro VC models in which both rat and human aortic vascular smooth muscle cells were treated with high levels of phosphate to mimic uremic hyperphosphatemia. Using an Affymetrix microRNA array, we found that miR-125b and miR-382 expression levels declined significantly as biomineralization progressed, but this decline was only observed for miR-125b in the culture medium...
May 18, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28514744/characterization-of-mouse-serum-exosomal-small-rna-content-the-origins-and-their-roles-in-modulating-inflammatory-response
#9
Xin Zhou, Zinan Jiao, Juling Ji, Shuyuan Li, Xiaodi Huang, Xiaoshuang Lu, Heng Zhao, Jingwen Peng, Xinya Chen, Qiuhong Ji, Yuhua Ji
In the last decade, although studies on exosomal microRNAs (miRNAs) derived from serum and other body fluids have increased dramatically; the contents and biological significance of serum exosomes under normal conditions remain unclear. In the present study, we profiled the small RNA content of mouse serum exosomes (mSEs) using small RNAseq and found that fragments of transfer RNAs (tRNAs) and miRNAs were the two predominant exosomal RNA species, accounting for approximately 60% and 10% of mapped reads, respectively...
April 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28514291/role-of-microrna-130a-in-the-pathogeneses-of-obstructive-sleep-apnea-hypopnea-syndrome-associated-pulmonary-hypertension-by-targeting-the-gax-gene
#10
Zhe An, Dan Wang, Guang Yang, Wen-Qi Zhang, Jin Ren, Jin-Ling Fu
OBJECTIVE: The purpose of this study was to elucidate the role of microRNA-130a (miR-130a) in obstructive sleep apnea hypopnea syndrome (OSAHS)-associated pulmonary hypertension (PHT) by targeting the growth arrest-specific homeobox (GAX) gene. METHODS: A total of 108 patients with OSAHS-associated PHT were recruited as the OSAHS-associated PHT group and 110 healthy individuals were randomly selected as the normal control group. Human umbilical vein endothelial cells (HUVECs) were selected and divided into the control, miR-130a mimic, mimic negative control (NC), miR-130a inhibitor, and inhibitor-NC groups...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28512857/interest-of-variations-in-mir-152-and-mir-122-in-a-series-of-hepatocellular-carcinomas-related-to-hcv-infection
#11
Elodie Miquelestorena-Standley, Anne Tallet, Christine Collin, Eric Piver, Anne De Muret, Ephrem Salamé, Pascal Bourlier, Thibault Kervarrec, Serge Guyétant, Jean-Christophe Pagès
AIM: Hepatocellular carcinoma (HCC) is a common outcome of chronic hepatitis C virus (HCV) infection and constitutes the main burden of this disease. The molecular mechanisms underlying the development of HCC are multiple and might involve certain microRNA (miR). Since discordant results have been reported concerning the detection of expression of miR-152 and miR-122 in HCC, our aim was to measure the levels of both miRs in serum and liver samples. METHODS: We analyzed miR-152 and miR-122 expression by RT-qPCR in a serum cohort from 14 HCV-infected patients who developed HCC, 20 HCV+ patients without HCC and 19 control patients...
May 17, 2017: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://www.readbyqxmd.com/read/28509372/differential-expression-of-micrornas-related-to-irritable-bowel-syndrome-in-a-rabbit-model
#12
Qinqin Yang, Xiaoping Xu, Hongshu Zhao, Yueqin Cai, Yongming Pan, Jianqin Xu, Quanxin Ma, Minli Chen
AIM: In the study we evaluated differential expression of miRNAs in the white hair and black eyes(WHBE) rabbit model of irritable bowel syndrome(IBS). METHODS: WHBE IBS rabbits were exposed to moist heat, stress, and stimulation with a low-dose laxative. Intestional propulsion rate(IPR) was measured. Blood samples were taken to detect changes in serum 5-hydroxytryptamine(5-HT) and dopamine(DA) levels, and colon tissues were to detect c-fos by immunohistochemistry...
May 16, 2017: Journal of Digestive Diseases
https://www.readbyqxmd.com/read/28503423/microrna-126-a-promising-novel-biomarker-in-peripheral-blood-for-diabetic-retinopathy
#13
Li-Li Qin, Mei-Xia An, Yan-Li Liu, Han-Chun Xu, Zhi-Qing Lu
AIM: To investigate the content of serum microRNA-126 (miR-126) and its role in screening retinal endothelial injury and early diagnosis of proliferative diabetic retinopathy. METHODS: The study included 184 serum samples, 59 samples from healthy individuals, 44 samples from diabetes mellitus (DM) patients without diabetic retinopathy (NDR), 42 from non-proliferative diabetic retinopathy (NPDR) patients and 39 samples from proliferative diabetic retinopathy (PDR) patients...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28501737/the-bcr-abl-tyrosine-kinase-inhibitor-nilotinib-stimulates-expression-of-il-1%C3%AE-in-vascular-endothelium-in-association-with-downregulation-of-mir-3p
#14
Masumi Sukegawa, Xiangmin Wang, Chie Nishioka, Bin Pan, Kailin Xu, Hiroshi Ohkawara, Yoichi Hamasaki, Masayuki Mita, Kenichi Nakamura, Masatoshi Okamoto, Hiromi Shimura, Masatsugu Ohta, Takayuki Ikezoe
BCR/ABL tyrosine kinase inhibitors (TKIs) have significantly improved the prognosis for in dividuals with chronic myeloid leukemia (CML). However, many patients treated with TKIs suffer from TKI-related complications. In particular, vascular events such as peripheral artery occlusive disease have become aserious clinical problem for patients who receive the TKI, nilotinib. At present, the molecular mechanisms by which TKIs cause vascular endothelial cell insults remain unknown.This study explored the effects of the TKIs, imatinib, nilotinib and dasatinib, on vascular endothelial cells in vitro, and found that only nilotinib induced expression of interleukin-1β (IL-1β) by vascular endothelial cells...
May 5, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28500393/association-of-serum-micrornas-with-islet-autoimmunity-disease-progression-and-metabolic-impairment-in-relatives-at-risk-of-type-1-diabetes
#15
Isaac V Snowhite, Gloria Allende, Jay Sosenko, Ricardo L Pastori, Shari Messinger Cayetano, Alberto Pugliese
AIMS/HYPOTHESIS: MicroRNAs (miRNAs) are key regulators of gene expression and novel biomarkers for many diseases. We investigated the hypothesis that serum levels of some miRNAs would be associated with islet autoimmunity and/or progression to type 1 diabetes. METHODS: We measured levels of 93 miRNAs most commonly detected in serum. This retrospective cohort study included 150 autoantibody-positive and 150 autoantibody-negative family-matched siblings enrolled in the TrialNet Pathway to Prevention Study...
May 12, 2017: Diabetologia
https://www.readbyqxmd.com/read/28499456/role-of-micrornas-in-premature-ovarian-insufficiency
#16
REVIEW
Ying Guo, Junyan Sun, Dongmei Lai
Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea lasting for a minimum of 4 months. The typical characteristics comprised of declined estrogen and raised serum concentrations of follicle-stimulating hormone (FSH) in women <40-year-old, primarily originating from iatrogenic factors, karyotypic abnormalities, and genetic factors. However, the etiology of POI remains unknown in approximately 90% of cases. POI could lead to infertility, osteoporosis, cardiovascular disorder, and cognitive dysfunction...
May 12, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28494871/mirna-32-drives-brown-fat-thermogenesis-and-trans-activates-subcutaneous-white-fat-browning-in-mice
#17
Raymond Ng, Nurul Attiqah Hussain, Qiongyi Zhang, Chengwei Chang, Hongyu Li, Yanyun Fu, Lei Cao, Weiping Han, Walter Stunkel, Feng Xu
Brown adipose tissue (BAT) activation and subcutaneous white fat browning are essential components of the thermogenic response to cold stimulus in mammals. microRNAs have been shown to regulate both processes in cis. Here, we identify miR-32 as a BAT-specific super-enhancer-associated miRNA in mice that is selectively expressed in BAT and further upregulated during cold exposure. Inhibiting miR-32 in vivo led to impaired cold tolerance, decreased BAT thermogenesis, and compromised white fat browning as a result of reduced serum FGF21 levels...
May 9, 2017: Cell Reports
https://www.readbyqxmd.com/read/28494029/characterization-of-mir-122-independent-propagation-of-hcv
#18
Chikako Ono, Takasuke Fukuhara, Daisuke Motooka, Shota Nakamura, Daisuke Okuzaki, Satomi Yamamoto, Tomokazu Tamura, Hiroyuki Mori, Asuka Sato, Kentaro Uemura, Yuzy Fauzyah, Takeshi Kurihara, Takahiro Suda, Akira Nishio, Su Su Hmwe, Toru Okamoto, Tomohide Tatsumi, Tetsuo Takehara, Kazuaki Chayama, Takaji Wakita, Kazuhiko Koike, Yoshiharu Matsuura
miR-122, a liver-specific microRNA, is one of the determinants for liver tropism of hepatitis C virus (HCV) infection. Although miR-122 is required for efficient propagation of HCV, we have previously shown that HCV replicates at a low rate in miR-122-deficient cells, suggesting that HCV-RNA is capable of propagating in an miR-122-independent manner. We herein investigated the roles of miR-122 in both the replication of HCV-RNA and the production of infectious particles by using miR-122-knockout Huh7 (Huh7-122KO) cells...
May 11, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28492809/serum-microrna-30c-levels-are-correlated-with-disease-progression-in-xinjiang-uygur-patients-with-chronic-hepatitis-b
#19
J Zhang, J Ma, H Wang, L Guo, J Li
We aimed to investigate the potential role and mechanism of microRNA-30c (miR-30c) in the pathological development of chronic hepatitis B (CHB). The serum levels of miR-30c in hepatitis B virus (HBV) carrier Xinjiang Uygur patients with inactive, low-replicative, high-replicative and HBe antigen-positive CHB were investigated. HepG2 cells were co-transfected with pHBV1.3 and miR-30c mimic or inhibitor or scramble RNA. The effects of miR-30c dysregulation on HBV replication and gene expression, cell proliferation and cell cycle were then investigated...
May 4, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28492505/mesoporous-silica-nanoparticles-as-carriers-for-intracellular-delivery-of-nucleic-acids-and-subsequent-therapeutic-applications
#20
REVIEW
Wenzhang Cha, Rengen Fan, Yufeng Miao, Yong Zhou, Chenglin Qin, Xiangxiang Shan, Xinqiang Wan, Jinbo Li
Nucleic acids, including DNA, microRNA (miRNA), small interfering RNA (siRNA), and antisense oligonucleotide (ASO), are powerful gene regulators, which have been demonstrated as promising drug candidates for therapeutic treatments. Nevertheless, poor cellular membrane permeability and serum stability have greatly hindered the applications of nucleic acids in biomedicine. To address these issues, associate carriers that can encapsulate and protect nucleic acids are urgently required. Mesoporous silica nanoparticles (MSNs or MSNPs), which are nanomaterials with excellent biocompatibility, large surface area for functionalization, and tunable pore size for encapsulating different cargos, are emerging as novel and ideal biomaterials for different biomedical applications...
May 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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