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Serum microRNA

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https://www.readbyqxmd.com/read/28748559/introduction-of-2-6-diaminopurines-into-serinol-nucleic-acid-sna-improves-anti-mirna-performance
#1
Yukiko Kamiya, Yuka Donoshita, Hiroshi Kamimoto, Keiji Murayama, Jumpei Ariyoshi, Hiroyuki Asanuma
MicroRNAs (miRNAs) are endogenous small RNAs that regulate gene expression at the post-transcriptional level by sequence-specific hybridization. Anti-miRNA oligonucleotides (AMOs) are inhibitors of miRNA activity. Chemical modifications of AMOs are required to increase binding affinity and stability in serum and cells. In this study we synthesized AMOs with our original acyclic nucleic acid, serinol nucleic acid (SNA) backbone and with an artificial nucleobase 2,6-diaminopurine. The AMO composed of only SNA had strong nuclease resistance and blocked endogenous miRNA activity...
July 26, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28742209/the-expression-significance-of-serum-mir-21-in-patients-with-osteosarcoma-and-its-relationship-with-chemosensitivity
#2
Y Hua, Z Jin, F Zhou, Y-Q Zhang, Y Zhuang
OBJECTIVE: To investigate the expression level of serum microRNA-21 (miR-21) in 69 patients with osteosarcoma, to analyze the changes in miR-21 expression in the serum and tumor tissues before and after chemotherapy in patients with osteosarcoma, and to investigate the relationship between the expression of serum miR-21 in patients with osteosarcoma and chemosensitivity. PATIENTS AND METHODS: Real-time polymerase chain reaction (RT-PCR) was used to detect the expression level of serum miR-21 in 69 patients with osteosarcoma before and after chemotherapy, and analyze the relationship between the expression of miR-21 with tumor necrosis grading and chemosensitivity...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28740547/down-regulated-mir-23a-contributes-to-the-metastasis-of-cutaneous-melanoma-by-promoting-autophagy
#3
Weinan Guo, Huina Wang, Yuqi Yang, Sen Guo, Weigang Zhang, Yu Liu, Xiuli Yi, Jingjing Ma, Tao Zhao, Lin Liu, Zhe Jian, Ling Liu, Gang Wang, Tianwen Gao, Qiong Shi, Chunying Li
Melanoma is among the most aggressive tumors, and the occurrence of metastasis leads to a precipitous drop in the patients' survival. Therefore, identification of metastasis-associated biomarkers and therapeutic targets will contribute a lot to improving melanoma theranostics. Recently, microRNAs (miRNAs) have been implicated in modulating cancer invasion and metastasis, and are proved as potential non-invasive biomarkers in sera for various tumors. Here, we reported miR-23a as a novel metastasis-associated miRNA that played a remarkable role in modulating melanoma invasive and metastatic capacity and was of great value in predicting melanoma metastasis and prognosis...
2017: Theranostics
https://www.readbyqxmd.com/read/28738533/mir-200a-expression-in-cd4-t-cells-correlates-with-the-expression-of-th17-treg-cells-and-relevant-cytokines-in-psoriasis-vulgaris-a-case-control-study
#4
Xiao-Yan Wang, Xing-Yu Chen, Jun Li, Hong-Ying Zhang, Juan Liu, Le-Dong Sun
The main aim of this study is to investigate the underlying relationship shared between microRNA-200a (miR-200a) and its link to concentrations of Th17 and Treg cells, mRNA expressions of their specific transcription factors retinoic acid-related orphan receptor γt (RORγt) and head box protein 3 (FOXP3) and relevant cytokines in patients with psoriasis vulgaris (PV). A total of 189 patients previously diagnosed with PV were selected as the experimental group, whilst 109 healthy individuals as the control group...
July 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738524/elevated-microrna-125b-promotes-inflammation-in-rheumatoid-arthritis-by-activation-of-nf-%C3%AE%C2%BAb-pathway
#5
Bo Zhang, Li-Song Wang, Yu-Hu Zhou
microRNA-125b (miR-125b) has been reported to be increased in rheumatoid arthritis (RA). However, the role of miR-125b in RA remains to be fully elucidated. We aimed to explore the functional role of miR-125b in RA, as well as the underlying mechanism. The expression of miR-125b in serum and synovial tissues of patients with RA and healthy controls was confirmed. In addition, the levels of miR-125b in lipopolysaccharide (LPS)-stimulated fibroblast-like synoviocytes (FLS) were also measured. FLS were transfected with miR-125b mimic, antisense oligonucleotides (ASO)-miR-125b, pcDNA-inhibitor of NF-κB (IκB)-α or corresponding controls, and then stimulated with LPS or incubated with nuclear factor kappa B (NF-κB) inhibitors pyrrolidine dithiocarbamate (PDTC)...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28732737/microrna-181a-and-its-target-smad-7-as-potential-biomarkers-for-tracking-child-acute-lymphoblastic-leukemia
#6
Marwa Nabhan, Manal L Louka, Eman Khairy, Fathy Tash, Randa Ali-Labib, Safinaz El-Habashy
Acute lymphoblastic leukemia (ALL) is the most common pediatric hematologic tumor. MiR-181a was expected to have a role in the development of hematological malignancies; it might act as tumor suppressor or oncogene. Smad7 was selected as miR-181a target pair. It is a negative regulator for the TGF-β1 signaling pathway. In this study, relative expression levels of miR-181a by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), both Smad 7 and TGF β1 proteins levels by enzyme linked immunosorbent assay (ELISA) were all measured in serum of 60 child, 30 with ALL and 30 age and sex matched healthy child as control group...
July 18, 2017: Gene
https://www.readbyqxmd.com/read/28731182/il%C3%A2-6-stat3-mir%C3%A2-34a-protects-against-neonatal-lung-injury-patients
#7
Huajun Liu, Wenbin Liu, Xueqing Tang, Taisen Wang, Xianlin Sun, Jie Lv
The present study aimed to investigate the protective role of the interleukin (IL)‑6/signal transducer and activator of transcription 3 (STAT3)/microRNA (miR)‑34a signaling pathway in patients with neonatal lung injury (NLI) and the underlying molecular mechanisms of these effects. It was demonstrated that miR‑34a serum expression was significantly upregulated in patients and mice with NLI. Meanwhile, IL‑6 and phosphorylated‑STAT3 protein expression, and tumor necrosis factor (TNF)‑α, IL‑1β and IL‑18 activity levels in NLI mice were significantly induced compared with the normal control group...
July 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28731129/expression-profile-of-c19mc-micrornas-in-placental-tissue-of-patients-with-preterm-prelabor-rupture-of-membranes-and-spontaneous-preterm-birth
#8
Ilona Hromadnikova, Katerina Kotlabova, Katarina Ivankova, Ladislav Krofta
The aim of the study was to demonstrate that preterm birth (PTB) is associated with altered C19MC microRNA expression profile in placental tissues. Gene expression of 15 placental specific microRNAs (miR‑512‑5p, miR‑515‑5p, miR‑516‑5p, miR‑517‑5p, miR‑518b, miR‑518f‑5p, miR‑519a, miR‑519d, miR‑519e‑5p, miR‑520a‑5p, miR‑520h, miR‑524‑5p, miR‑525‑5p, miR‑526a and miR‑526b‑5p) was compared between groups: 34 spontaneous PTB, 108 preterm prelabor rupture of membranes (PPROM) and 20 term in labor pregnancies...
July 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28723645/altered-micrornas-expression-in-t-cells-of-patients-with-sle-involved-in-the-lack-of-vitamin-d
#9
Dao-Jun Chen, Lan-Ju Li, Xiao-Ke Yang, Tao Yu, Rui-Xue Leng, Hai-Feng Pan, Dong-Qing Ye
Vitamin D has been recognized as a potent immunomodulator and its deficiency is common in different population groups including patients with SLE. As miRNAs regulation plays a significant role in SLE, the present study aimed to evaluate the association between vitamin D status and miRNAs levels in patients with SLE. The serum concentrations of vitamin D (25-hydroxyvitamin D) and the levels of six miRNAs in T cells from patients with SLE were measured in 42 SLE cases and 48 healthy controls. Vitamin D treatment was also performed in isolated and cultured T cells from SLE patients in different times and doses...
July 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28722430/multi-amplified-sensing-of-microrna-by-a-small-dna-fragment-driven-enzymatic-cascade-reaction
#10
Eunjung Kim, Philip D Howes, Spencer W Crowder, Molly M Stevens
Combining technological developments such as nanomaterials, DNA nanotechnology, and functional enzymes has great potential to facilitate next generation high performance molecular diagnostic systems. In this work, we describe a microRNA (miRNA) detection assay that combines target recycling and isothermal amplification in an elegantly designed enzyme-mediated cascade reaction. Target recycling is driven by the action of duplex-specific nuclease (DSN), resulting in highly amplified translation of input miRNA to short output DNA fragments...
January 27, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28719617/microrna-155-deficiency-leads-to-decreased-autoantibody-levels-and-reduced-severity-of-nephritis-and-pneumonitis-in-pristane-induced-lupus
#11
Harald Leiss, Wilhelm Salzberger, Barbara Jacobs, Irina Gessl, Nicolas Kozakowski, Stephan Blüml, Antonia Puchner, Attila Kiss, Bruno K Podesser, Josef S Smolen, Georg H Stummvoll
OBJECTIVE: We herein examine the role of endogenous miR155 in the development of systemic manifestations in pristane induced lupus. MATERIALS AND METHODS: Systemic lupus in miR155-deficient and wild type mice was induced upon injection of pristane and analyzed after 8 months, PBS-injected mice served as controls. Glomerulonephritis and pneumonitis were quantified using the kidney biopsy score and a newly adapted histomorphometric image analysis system; lung tissue was further analyzed by tissue cytometry...
2017: PloS One
https://www.readbyqxmd.com/read/28719355/mirna-223-suppresses-foxo1-and-functions-as-a-potential-tumor-marker-in-breast-cancer
#12
Y-T Wei, D-W Guo, X-Z Hou, D-Q Jiang
Breast cancer is the most commonly diagnosed cancer in women and a leading cause of cancer mortality. MicroRNAs (miRNAs) have been found to play a key role in proliferation, metastasis and invasion of cancer. In previous study, we found that miRNA-223 was significant expression inexosome derived from peripheral blood serum of breast cancer patients than in samples from control subjects, Therefor,the role ofmiRNA-223willbe researched in MCF-7 breast cancer cells.In this study, to explore the role of miRNA-223in influencing cell proliferation, metastasis and invasion of breast cancer, TargetScan tools (http://www...
May 20, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28713918/endothelial-growth-medium-suppresses-apoptosis-of-mesenchymal-stem-cells-in-vitro-via-decrease-of-mir%C3%A2-29a
#13
Qianqian Wu, Tao Fang, Min Chen, Guoxian Qi
The administration of mesenchymal stem cells (MSCs) in cases of cardiac ischemia/reperfusion injury (IRI) has been associated with a significant reduction of myocardial cell death and an effective improvement in cardiac function. However, one major limiting factor in MSCs transplantation therapy is the low survival rate of the transplanted cells. The present study aimed to demonstrate that human amnion‑derived mesenchymal stem cells (hAMSCs) cultured with endothelial growth medium (EGM‑2) exhibited reduced apoptosis when exposed to serum‑free and hypoxic conditions; and that the expression of microRNA (miR)‑29a decreased significantly...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28710399/downregulation-of-blood-serum-microrna-29-family-in-patients-with-parkinson-s-disease
#14
Xiaochen Bai, Yilin Tang, Mei Yu, Lei Wu, Fengtao Liu, Jianliang Ni, Zishan Wang, Jinghui Wang, Jian Fei, Wei Wang, Fang Huang, Jian Wang
There is currently no reliable and easily applicable diagnostic marker for Parkinson's disease (PD). The aims of the present study were to compare the expression profiles of the microRNA29 family (miR-29s) in blood serum from patients with PD with healthy controls and to clarify whether the expression of miR-29s is correlated with disease severity, duration or L-dopa therapy and whether expression depends on the gender and age of patients. The levels of blood serum miR-29s in 80 patients with PD and 80 unaffected controls were assessed by reverse transcription-quantitative real-time PCR...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28709088/integrated-amplified-aptasensor-with-in-situ-precise-preparation-of-copper-nanoclusters-for-ultrasensitive-electrochemical-detection-of-microrna-21
#15
Yijia Wang, Xinying Zhang, Lang Zhao, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang
MicroRNA 21 (MIR21) has garnered much attention in recent years as an important disease biomarker. The detection of it in human system shows great significance for the healthy evaluation and major diseases early detection. Herein, a novel approach tactfully manipulates the in-situ precise preparation of copper nanoclusters on overlapping Y-shaped ds-DNA for MIR21 analysis were developed in the proposed integrated aptasensor. In the presence of target MIR21, overlapping Y-shaped ds-DNA was constructed on electrode...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28707877/free-energy-driven-lock-open-assembly-based-optical-dna-sensor-for-cancer-related-microrna-detection-with-a-shortened-time-to-result
#16
Xiyu Zhu, Ruoyu Wang, Xiaohong Zhou, Hanchang Shi
Quantification of cancer biomarker microRNAs (miRs) by exquisitely designed biosensors with a short time-to-result is of great clinical significance. With immobilized capture probes (CPs) and fluorescent-labeled signal probes (SPs), surface-involved sandwich-type (SST) biosensors serve as powerful tools for rapid, highly sensitive, and selective detection of miR in complex matrices as opposed to the conventional techniques. One key challenge for such SST biosensors is the existence of false-negative signals when the amount of miRs exceeds SPs in solution phase for a surface with a limited number of CP...
July 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28704935/entrainment-of-breast-cell-lines-results-in-rhythmic-fluctuations-of-micrornas
#17
Rafael Chacolla-Huaringa, Jorge Moreno-Cuevas, Victor Trevino, Sean-Patrick Scott
Circadian rhythms are essential for temporal (~24 h) regulation of molecular processes in diverse species. Dysregulation of circadian gene expression has been implicated in the pathogenesis of various disorders, including hypertension, diabetes, depression, and cancer. Recently, microRNAs (miRNAs) have been identified as critical modulators of gene expression post-transcriptionally, and perhaps involved in circadian clock architecture or their output functions. The aim of the present study is to explore the temporal expression of miRNAs among entrained breast cell lines...
July 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28702050/circulating-micrornas-in-serum-from-cattle-challenged-with-bovine-viral-diarrhea-virus
#18
Tasia M Taxis, Fernando V Bauermann, Julia F Ridpath, Eduardo Casas
Bovine viral diarrhea virus (BVDV) is an RNA virus that is often associated with respiratory disease in cattle. MicroRNAs have been proposed as indicators of exposure to respiratory pathogens. The objective of this study was to identify microRNAs in cattle that had been challenged with a non-cytopathic field strain of BVDV. Five colostrum deprived neonate Holstein calves were inoculated with BVDV (challenged) and 4 were mock challenged (control). Serum from all calves was collected at four different times: prior to challenge (day 0) and at 4, 9, and 16 days post-challenge...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28701355/mir-4632-mediates-pdgfbb-induced-proliferation-and-anti-apoptosis-of-human-pulmonary-artery-smooth-muscle-cells-via-targeting-cjun
#19
Zhengjiang Qian, Yanjiao Li, Jidong Chen, Xiang Li, Deming Gou
MicroRNAs (miRNAs) can regulate proliferative status of pulmonary artery smooth muscle cells (PASMCs), which is a core factor modulating pulmonary vascular remodeling diseases, such as atherosclerosis and pulmonary arterial hypertension (PAH). Our previous work has shown that miR-4632, a rarely reported miRNA, is significantly downregulated in Platelet-Derived Growth Factor (PDGF)BB-stimulated human pulmonary artery smooth muscle cells (HPASMCs), yet its cell function and the underlying molecular mechanisms remain to be elucidated...
July 12, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28700503/mir-210-and-mir-155-as-potential-diagnostic-markers-for-pre-eclampsia-pregnancies
#20
Lu Gan, Zheng Liu, Ming Wei, Yulong Chen, Xiaomei Yang, Lihong Chen, Xiaomin Xiao
Pre-eclampsia (PE) is one of the leading causes of maternal and neonatal morbidity and mortality. In recent years, many studies have shown that microRNAs (miRNA) play important roles in the development of PE. However, the molecular pathogenesis of PE remains unknown.In the present study, we performed a case-control study to verify the differential expression of 4 candidate miRNAs (miR-210, miR-155, miR-125b-5p, and miR-125a-5p) in 20 PE pregnancies and 20 healthy pregnancies. The real-time quantitative reverse transcriptase-polymerase chain reaction has been utilized to estimate the Ct values in both groups...
July 2017: Medicine (Baltimore)
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