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Serum microRNA

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https://www.readbyqxmd.com/read/28927067/expression-of-mir-146a-in-patients-with-ovarian-cancer-and-its-clinical-significance
#1
Miłosz Wilczyński, Ewelina Żytko, Bożena Szymańska, Monika Dzieniecka, Marek Nowak, Justyna Danielska, Grzegorz Stachowiak, Jacek R Wilczyński
The aim of the present retrospective study was to compare microRNA (miR)-146a expression levels in primary tumors and omental metastases of 48 patients, who had undergone surgery for advanced ovarian serous cancer. Possible correlations between miR-146a expression level and clinicopathological features were investigated, including chemosensitivity and survival. miR-146a was evaluated in formalin-fixed, paraffin-embedded samples. miR-146a expression level in primary tumors was demonstrated to be increased in comparison with normal ovary tissues (P=0...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28919434/ultrasensitive-microrna-21-detection-based-on-dna-hybridization-chain-reaction-and-sybr-green-dye
#2
Zhi Li, Bingchen Li, Yunlei Zhou, Huanshun Yin, Jun Wang, Shiyun Ai
It is extremely important for quantifying trace microRNAs in the biomedical applications. In this study, an ultrasensitive, rapid and efficient label-free fluorescence method was proposed and applied for detecting microRNA-21 in serum of gastric cancer patients based on DNA hybridization chain reaction (HCR). DNA H1 and DNA H2 were designed and used as hairpin probes, the HCR was proceeded in the presence of target microRNAs. Amounts of SYBR Green І dyes were used as signal molecules to intercalate long DNA concatemers from HCR, which guaranteed the model of label-free fluorescence and strong fluorescence density...
September 15, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28917266/urinary-biomarkers-in-prostate-cancer-detection-and-monitoring-progression
#3
REVIEW
Duojia Wu, Jie Ni, Julia Beretov, Paul Cozzi, Mark Willcox, Valerie Wasinger, Bradley Walsh, Peter Graham, Yong Li
Prostate cancer (CaP) is the most common cancer in men and the second leading cause of cancer deaths in males in Australia. Although serum prostate-specific antigen (PSA) has been the most widely used biomarker in CaP detection for decades, PSA screening has limitations such as low specificity and potential association with over-diagnosis. Current biomarkers used in the clinic are not useful for the early detection of CaP, or monitoring its progression, and have limited value in predicting response to treatment...
October 2017: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/28914881/microrna-200c-impairs-uterine-receptivity-formation-by-targeting-fut4-and-%C3%AE-1-3-fucosylation
#4
Qin Zheng, Dandan Zhang, Y U Yang, Xinyuan Cui, Jiaqi Sun, Caixia Liang, Huamin Qin, Xuesong Yang, Shuai Liu, Qiu Yan
Successful embryo implantation requires the establishment of a receptive endometrium. Poor endometrial receptivity has generally been considered as a major cause of infertility. Protein glycosylation is associated with many physiological and pathological processes. The fucosylation is catalyzed by the specific fucosyltransferases. Fucosyltransferase IV (FUT4) is the key enzyme for the biosynthesis of α1,3-fucosylated glycans carried by glycoproteins, and the previous studies showed FUT4 expression changed dynamically during perimplantation...
September 15, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28904856/mir-101-inhibits-ovarian-carcinogenesis-by-repressing-the-expression-of-brain-derived-neurotrophic-factor
#5
Ying Xu, Lei Xu, Jianbin Zheng, Lei Geng, Shuping Zhao
Ovarian cancer is one of the most lethal malignant gynecological tumors as a result of difficulties in early-stage detection and a lack of effective treatments for patients with advanced or recurrent cancer. In the present study, we aimed to explore whether some of the microRNA (miRNA) content of serum might be related to ovarian cancer, as well as the role of these miRNAs and their intercellular transport via exosomes in ovarian cancer. We first detected the expression of six candidate miRNAs in ovarian cancer tissues and adjacent nontumor ovarian samples from 36 patients and confirmed the altered expression of four miRNAs...
September 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28900486/upregulation-of-serum-mir-10b-is-associated-with-poor-prognosis-in-patients-with-melanoma
#6
Ming Bai, Hailin Zhang, Loubin Si, Nanze Yu, Ang Zeng, Ru Zhao
Aberrant expression of microRNAs (miRNAs) are believed to play a central role in the initiation and development of cancer. The aim of our study was to determine the clinical significance of serum miR-10b in melanoma. A total of 85 and 30 serum samples were obtained from patients with melanoma and healthy volunteers respectively. qRT-PCR was performed to evaluate the expression level of miR-10b in the melanoma cell lines and the serum samples from the participants. Then the clinical significance of serum miR-10b was further determined...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28891208/serum-microrna-150-predicts-prognosis-for-early-stage-non-small-cell-lung-cancer-and-promotes-tumor-cell-proliferation-by-targeting-tumor-suppressor-gene-srcin1
#7
Liren Zhang, Jing Lin, Yuanqing Ye, Taro Oba, Emanuela Gentile, Jie Lian, Jing Wang, Yang Zhao, Jian Gu, Ignacio I Wistuba, Jack A Roth, Lin Ji, Xifeng Wu
This integrative multistage study was aimed to identify circulating miRNAs as prognostic biomarkers and investigate treatment target for early-stage non-small cell lung cancer (NSCLC) patients. In stage I-II NSCLC patients, we screened and validated miRNA ratio signatures predictive of prognosis in serum. In tumor, we found that the expression of miR-150 in identified miRNA signatures was also associated with survival. Increased miR-150 expression promoted NSCLC cell proliferation and migration and vice versa...
September 10, 2017: Clinical Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28886070/microrna-mir-301a-is-a-novel-cardiac-regulator-of-cofilin-2
#8
Ashraf Yusuf Rangrez, Phillip Hoppe, Christian Kuhn, Elisa Zille, Johanne Frank, Norbert Frey, Derk Frank
Calsarcin-1 deficient mice develop dilated cardiomyopathy (DCM) phenotype in pure C57BL/6 genetic background (Cs1-ko) despite severe contractile dysfunction and robust activation of fetal gene program. Here we performed a microRNA microarray to identify the molecular causes of this cardiac phenotype that revealed the dysregulation of several microRNAs including miR-301a, which was highly downregulated in Cs1-ko mice compared to the wild-type littermates. Cofilin-2 (Cfl2) was identified as one of the potential targets of miR-301a using prediction databases, which we validated by luciferase assay and mutation of predicted binding sites...
2017: PloS One
https://www.readbyqxmd.com/read/28882868/genetic-ablation-of-microrna-33-attenuates-inflammation-and-abdominal-aortic-aneurysm-formation-via-several-anti-inflammatory-pathways
#9
Tetsushi Nakao, Takahiro Horie, Osamu Baba, Masataka Nishiga, Tomohiro Nishino, Masayasu Izuhara, Yasuhide Kuwabara, Hitoo Nishi, Shunsuke Usami, Fumiko Nakazeki, Yuya Ide, Satoshi Koyama, Masahiro Kimura, Naoya Sowa, Satoko Ohno, Hiroki Aoki, Koji Hasagawa, Kazuhisa Sakamoto, Kenji Minatoya, Takeshi Kimura, Koh Ono
OBJECTIVE: Abdominal aortic aneurysm (AAA) is an increasingly prevalent and ultimately fatal disease with no effective pharmacological treatment. Because matrix degradation induced by vascular inflammation is the major pathophysiology of AAA, attenuation of this inflammation may improve its outcome. Previous studies suggested that microRNA-33 inhibition and genetic ablation of microRNA-33 increased serum high-density lipoprotein cholesterol and attenuated atherosclerosis. APPROACH AND RESULTS: MicroRNA-33a-5p expression in central zone of human AAA was higher than marginal zone...
September 7, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28881648/correlation-between-microrna-143-in-peripheral-blood-mononuclear-cells-and-disease-severity-in-patients-with-psoriasis-vulgaris
#10
Yi-Zhi Zheng, Chun-Feng Chen, Li-Ying Jia, Tu-Gen Yu, Jie Sun, Xiao-Yong Wang
This study aims to explore the correlation between microRNA-143 (miR-143) expression in peripheral blood mononuclear cells (PBMCs) and disease severity in patients with psoriasis vulgaris. From March 2014 to November 2015, 194 patients with psoriasis vulgaris (102 patients in progressive stage and 92 patients in stable stage) were selected as the case group and 175 healthy people as a control group were enrolled in this study. ELISA was used to detect the levels of IL-17 and VEGF in serum. The qRT-PCR assay was performed to detect the relative expression of miR-143 in PBMCs...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28878865/micrornas-and-clinical-implications-in-hepatocellular-carcinoma
#11
Amal Ahmed Mohamed, Zainab A Ali-Eldin, Tamer A Elbedewy, Magdy El-Serafy, Fatma A Ali-Eldin, Hossameldin AbdelAziz
AIM: To assess the role of some circulating miRNAs (miR-23a, miR-203, miR338, miR-34, and miR-16) as tumor markers for diagnosis of hepatocellular carcinoma (HCC). METHODS: One hundred and seventy-one subjects were enrolled, 57 patients with HCC, 57 patients with liver cirrhosis (LC) and 57 healthy subjects as control group. Severity of liver disease was assessed by Child Pugh score. Tumor staging was done using Okuda staging system. Quantification of Micro RNA (miR-23a, miR-203, miR338, miR-34, and miR-16) was performed...
August 18, 2017: World Journal of Hepatology
https://www.readbyqxmd.com/read/28878369/peroxisome-proliferator-activated-receptor-gamma-in-obesity-and-colorectal-cancer-the-role-of-epigenetics
#12
T K Motawi, O G Shaker, M F Ismail, N H Sayed
Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor that is deregulated in obesity. PPARγ exerts diverse antineoplastic effects. Attempting to determine the clinical relevance of the epigenetic mechanisms controlling the expression PPARγ and susceptibility to colorectal cancer (CRC) in obese subjects, this study investigated the role of some microRNAs and DNA methylation on the deregulation of PPARγ. Seventy CRC patients (34 obese and 36 lean), 22 obese and 24 lean healthy controls were included...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28872300/ultra-sensitive-microrna-assay-via-surface-plasmon-resonance-responses-of-au-ag-nanorods-etching
#13
Yu Gu, Juan Song, Mei-Xing Li, Tingting Zhang, Wei Zhao, Jing-Juan Xu, Maili Liu, Hong-Yuan Chen
Quantification of trace serum circulate microRNAs is extremely important in clinical diagnosis but remains a great challenge. Herein we developed an ultra-sensitive platform for microRNA 141 (miR-141) detection based on a silver coated gold nanorods (Au@Ag NRs) etching process accompanied by surface plasmon resonance (SPR) shift. Both SPR absorption and scattering responses were monitored. Combined amplification cascades of catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) with the sensitive SPR responses of plasmonic Au@Ag NRs, the proposed bioassay exhibited ultra-high sensitivity toward miRNA-141 with dynamic range from 5...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28869447/high-concentration-of-mir-133-is-a-useful-marker-for-the-diagnosis-of-lymphoma-associated-hemophagocytic-syndrome
#14
Wuping Li, Yun Zhong, Yuerong Shuang, Hui Huang, Yan Huang, Li Yu, Xianbao Huang
BACKGROUND: Lymphoma associated hemophagocytic syndrome (LAHS) is one of the major adult secondary hemophagocytic lymphohistiocytosis (HLH). Early diagnosis and treatment contribute to improved outcome. No enlarge lymph nodes can often delay the diagnosis of underlying lymphoma. OBJECTIVE: To find out criteria distinguishing LAHS from HLH induced by benign diseases. METHODS: clinical characteristic and laboratory feature of 31 patients with HLH (10 benign disease-associated HLH and 21 LAHS) were analyzed retrospectively...
August 23, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28864214/microrna-218-acts-by-repressing-tnfr1-mediated-activation-of-nf-%C3%AE%C2%BAb-which-is-involved-in-muc5ac-hyper-production-and-inflammation-in-smoking-induced-bronchiolitis-of-copd
#15
Hui Xu, Qian Sun, Lu Lu, Fei Luo, Liang Zhou, Jianping Liu, Lei Cao, Qiushi Wang, Junchao Xue, Qianlei Yang, Ping Yang, Jiachun Lu, Quanyong Xiang, Qizhan Liu
Dysregulation of microRNAs (miRNAs) has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD), which is largely attributable to cigarette smoke (CS). However, little is known about the effect of miRNAs on CS-induced mucus hypersecretion and the inflammatory response in the airway epithelium, which are pathological characteristics of COPD-related chronic bronchiolitis. As determined in the present investigation, population-based data indicate that smokers with COPD had serious airflow obstruction and inflammation, whereas smokers without COPD had mild airflow obstruction and inflammation...
August 31, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28862713/meta-analysis-of-micrornas-as-potential-biomarkers-for-detecting-esophageal-carcinoma-in-asian-populations
#16
Mei Li, Fei Wu, Yu Ji, Lan Yang, Feng Li
BACKGROUND: An Increasing number of studies in the literature have shown that microRNAs (miRNAs) can be used as early diagnostic markers for esophageal carcinoma (EC), but their conclusions remain controversial. Hence, we performed this meta-analysis to evaluate the diagnostic accuracy of using miRNAs in EC and to provide an experimental basis for early diagnosis of the disease. METHODS: This meta-analysis included 39 Asian studies from 18 articles, which covered 3,708 EC patients and 2,689 healthy controls...
August 28, 2017: International Journal of Biological Markers
https://www.readbyqxmd.com/read/28854209/identification-of-a-serum-induced-transcriptional-signature-associated-with-metastatic-cervical-cancer
#17
Anna Palatnik, Shuyun Ye, Christina Kendziorski, Marissa Iden, Jessica S Zigman, Martin J Hessner, Janet S Rader
OBJECTIVE: Tumor cells that escape local tissue control can convert inflammatory cells from tumor suppressors to tumor promoters. Moreover, soluble immune-modulating factors secreted from the tumor environment can be difficult to identify in patient serum due to their low abundance. We used an alternative strategy to infer a metastatic signature induced by sera of cervical cancer patients. METHODS: Sera from patients with local and metastatic cervical cancer were used to induce a disease-specific transcriptional signature in cultured, healthy peripheral blood mononuclear cells (PBMCs)...
2017: PloS One
https://www.readbyqxmd.com/read/28852755/intelligent-identification-of-multi-level-nanopore-signatures-for-accurate-detection-of-cancer-biomarkers
#18
Jian-Hua Zhang, Xiu-Ling Liu, Zheng-Li Hu, Yi-Lun Ying, Yi-Tao Long
To achieve accurate detection of cancer biomarkers with nanopore sensors, the precise recognition of multi-level current blockage events (signature) is a pivotal problem. However, it remains rather a challenge to identify the multi-level current blockages of target biomarkers in nanopore experiments, especially for the nanopore analysis of serum samples. In this work, we combined a modified DBSCAN (Density-Based Spatial Clustering of Applications with Noise) algorithm with the Viterbi training algorithm of the hidden Markov model (HMM) to achieve intelligent retrieval of multi-level current signatures from microRNA in serum samples...
August 30, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28852498/serum-micrornas-are-altered-in-various-stages-of-chronic-kidney-disease-a-preliminary-study
#19
Benjamin Brigant, Valérie Metzinger-Le Meuth, Ziad A Massy, Nathalie McKay, Sophie Liabeuf, Marion Pelletier, Marion Sallée, Eléonore M'Baya-Moutoula, Pascale Paul, Tilman B Drueke, Stéphane Burtey, Laurent Metzinger
[This retracts the article DOI: 10.1093/ckj/sfw060.].
August 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28845337/a-carbon-nanotube-reporter-of-mirna-hybridization-events-in-vivo
#20
Jackson D Harvey, Prakrit V Jena, Hanan A Baker, Gül H Zerze, Ryan M Williams, Thomas V Galassi, Daniel Roxbury, Jeetain Mittal, Daniel A Heller
MicroRNAs and other small oligonucleotides in biofluids are promising disease biomarkers, yet conventional assays require complex processing steps that are unsuitable for point-of-care testing or for implantable or wearable sensors. Single-walled carbon nanotubes are an ideal material for implantable sensors, owing to their emission in the near-infrared spectral region, photostability and exquisite sensitivity. Here, we report an engineered carbon-nanotube-based sensor capable of real-time optical quantification of hybridization events of microRNA and other oligonucleotides...
2017: Nature biomedical engineering
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