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Lixia Fu, Jing Guan, Yujia Zhang, Pei Ma, Yuanyuan Zhuang, Jinye Bai, Yasi Ding, Qi Hou, Wan Gong, Mingbao Lin, Wensheng Zheng, Jianmin Zhang
Background: Tulobuterol patch, one of strongest bronchodilators, was recently shown to improve bronchial hyperresponsiveness and significantly decrease the sputum eosinophil counts by combining with nonspecific anti-inflammatory drugs on patients with asthma. However, there is limited study on the anti-inflammatory activities of tulobuterol patch and its potential machenism. Results: The tulobuterol patch significantly ameliorated inflammatory cell infiltration in the lung tissue, reduced the number of total leukocytes and its differential count, markedly reduced the production of IL-1β, TNF-α, IL-6, CCL-11 and IL-4 in bronchial alveolar lavage fluid, as well as a reduction in IL-4/IFN-γ ratio...
February 23, 2018: Oncotarget
De-Gang Ji, Yan Zhang, Song-Mei Yao, Xu-Jie Zhai, Li-Rong Zhang, Yao-Zhong Zhang, Hui Li
Caveolin-1 (Cav-1), as a membrane protein involved in the formation of caveolae, binds steroid receptors and endothelial nitric oxide synthase, limiting its translocation and activation. In the present study, we investigated the role of Cav-1 in the progression of hepatic fibrosis induced by carbon tetrachloride (CCl4 ) in murine animals. Therefore, the wild type (WT) and Cav-1-knockout (Cav-1-/- ) mice were used in our study and subjected to CCl4 . The results indicated that CCl4 induced the decrease of Cav-1 expression in liver tissue samples...
March 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Fang-Tian Bu, Yu Chen, Hai-Xia Yu, Xin Chen, Yang Yang, Xue-Yin Pan, Qin Wang, Yu-Ting Wu, Cheng Huang, Xiao-Ming Meng, Jun Li
SUMOylation and deSUMOylation, a dynamic process, is proved to be involved in various fibrotic diseases. Here, we found SENP2, one of deSUMOylation protease family member, was decreased in CCl4 -induced mice fibrotic liver tissues, primary HSCs and restored after spontaneously recovery. In addition, HSC-T6 cells with TGF-β1 treatment resulted in a significant reduction of SENP2. Ectopic expression of SENP2 hindered cells activation and proliferation induced by TGF-β1 while knockdown of SENP2 showed an opposite effect...
March 10, 2018: Toxicology Letters
Zhonghua Li, Wei Zeng, Shiyi Ye, Jian Lv, Axiu Nie, Bingzhou Zhang, Yumei Sun, Heyou Han, Qigai He
The nucleocapsid (N) protein is a major structural component of porcine epidemic diarrhea virus (PEDV), which is predicted to be a multifunctional protein in viral replication. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a cellular protein participating in the splicing of pre-mRNA in the nucleus and translation regulation in the cytoplasm. According to our previous proteomic study about PEDV infection in vivo, hnRNP A1 was thought to be a cellular factor influencing PEDV replication. In this report, PEDV N protein was discovered to colocalize with cellular hnRNP A1 in perinuclear region of PEDV infected cells...
March 13, 2018: Viruses
Yoshihito Shima, Yasushi Kawaguchi, Masataka Kuwana
OBJECTIVES: To evaluate the anti-interleukin(IL)-6 receptor antibody tocilizumab (TCZ) as a treatment of systemic sclerosis (SSc), a randomized parallel group study was conducted, and compared their results and baseline cytokine/chemokine profiles. METHODS: Patients were assigned to a TCZ add-on group (TCZ group, n = 7) and a conventional therapy group (Conv group, n = 6). TCZ (8 mg/kg/month) for 6 months, and the modified Rodnan total skin score (mRSS) was used to compare the efficacy...
March 13, 2018: Modern Rheumatology
Sheng-Hsun Hsieh, Yung-Hui Li, Wei Wang, Chung-Hao Tien
In this study, we maneuvered a dual-band spectral imaging system to capture an iridal image from a cosmetic-contact-lens-wearing subject. By using the independent component analysis to separate individual spectral primitives, we successfully distinguished the natural iris texture from the cosmetic contact lens (CCL) pattern, and restored the genuine iris patterns from the CCL-polluted image. Based on a database containing 200 test image pairs from 20 CCL-wearing subjects as the proof of concept, the recognition accuracy (False Rejection Rate: FRR) was improved from FRR = 10...
March 6, 2018: Sensors
Wendy I Baltzer, Sarah Smith-Ostrin, Jennifer J Warnock, Craig G Ruaux
OBJECTIVE To assess clinical effects of an omega-3 fatty acid and protein-enriched diet, physical rehabilitation, or both in dogs following tibial plateau leveling osteotomy (TPLO) and arthroscopic surgery for cranial cruciate ligament (CCL) disease. DESIGN Randomized, prospective clinical trial. ANIMALS 48 dogs with unilateral CCL disease. PROCEDURES Dogs were randomly assigned to receive a dry omega-3 fatty acid and protein-enriched dog food formulated to support joint health (test food [TF]), a dry food formulated for maintenance of adult dogs (control food [CF]), TF plus rehabilitation (TF-R), or CF plus rehabilitation (CF-R)...
March 15, 2018: Journal of the American Veterinary Medical Association
Chun-Miao Ji, Bin Wang, Jiyong Zhou, Yao-Wei Huang
A monkey cell line Vero (ATCC CCL-81) is commonly used for porcine epidemic diarrhea virus (PEDV) propagation in vitro. However, it is still controversial whether the porcine aminopeptidase N (pAPN) counterpart on Vero cells (Vero-APN) confers PEDV entry. We found that endogenous expression of Vero-APN was undetectable in the mRNA and the protein levels in Vero cells. We cloned the partial Vero-APN gene (3340-bp) containing exons 1 to 9 from cellular DNA and subsequently generated two APN-knockout Vero cell lines by CRISPR/Cas9 approach...
March 1, 2018: Virology
Haitao Sun, Guanxin Chen, Bin Wen, Jialing Sun, Haiyan An, Jie Pang, Wei Xu, Xuemei Yang, Songqi He
Oligo-peptide I-C-F-6 is a Carapax trionycis extract component that has an effect on hepatic fibrosis, however, its mechanism of action is still unclear. This study investigated whether oligo-peptide I-C-F-6 could inhibit liver fibrosis by suppressing NF-κB and Wnt/β-catenin signaling, which are important in liver fibrosis. HSC-T6 cells were treated with oligo-peptide I-C-F-6, and rats were divided randomly into five groups: control (saline), CCl4 , CCl4 plus oligo-peptide I-C-F-6 (0.12 and 0.24 mg/kg), and CCl4 plus colchicine (0...
February 2, 2018: Journal of Pharmacological Sciences
Lovorka Grgurevic, Igor Erjavec, Ivica Grgurevic, Ivo Dumic-Cule, Jelena Brkljacic, Donatella Verbanac, Mario Matijasic, Hana Cipcic Paljetak, Rudjer Novak, Mihovil Plecko, Jadranka Bubic-Spoljar, Dunja Rogic, Vera Kufner, Martina Pauk, Tatjana Bordukalo-Niksic, Slobodan Vukicevic
Liver fibrosis is a progressive pathological process resulting in an accumulation of excess extracellular matrix proteins. We discovered that bone morphogenetic protein 1-3 (BMP1-3), an isoform of the metalloproteinase Bmp1 gene, circulates in the plasma of healthy volunteers and its neutralization decreases the progression of chronic kidney disease in 5/6 nephrectomized rats. Here, we investigated the potential role of BMP1-3 in a chronic liver disease. Rats with carbon tetrachloride (CCl4 )-induced liver fibrosis were treated with monoclonal anti-BMP1-3 antibodies...
February 27, 2018: Growth Factors
Rajesh Parmar, Hardik Patel, Naveen Yadav, Ritika Parikh, Khyati Patel, Aditi Mohankrishnan, Vishakha Bhurani, Urja Joshi, Sarat Kumar Dalai
Immunization with radiation-attenuated sporozoites (RAS) shown to confer complete sterile protection against Plasmodia liver-stage (LS) infection that lasts about 6 to 9 months in mice. We have found that the intermittent infectious sporozoite challenge to immune mice following RAS vaccination extends the longevity of sterile protection by maintaining CD8+ T cell memory responses to LS infection. It is reported that CD8α+ dendritic cells (DCs) are involved in the induction of LS-specific CD8+ T cells following RAS or genetically attenuated parasite (GAP) vaccination...
2018: Frontiers in Immunology
Daniela Dörfel, Christian J Lechner, Simone Joas, Tanja Funk, Michael Gutknecht, Julia Salih, Julian Geiger, Korbinian N Kropp, Stefanie Maurer, Martin R Müller, Hans-Georg Kopp, Helmut R Salih, Frank Grünebach, Susanne M Rittig
In chronic myeloid leukemia (CML), the translocation t(9;22) results in the fusion protein BCR-ABL (breakpoint cluster region-abelson murine leukemia), a tyrosine kinase mediating oncogenic signaling which is successfully targeted by treatment with BCR-ABL inhibitors like imatinib. However, BCR-ABL inhibitors may also affect antitumor immunity. For instance, it was reported that imatinib impairs the function of dendritic cells (DCs) that play a central role in initiating and sustaining T cell responses. Meanwhile, second generation BCR-ABL inhibitors like nilotinib, which inhibits BCR-ABL with enhanced potency have become standard of treatment, at least in patients with BCR-ABL kinase domain mutations...
February 21, 2018: Cancer Immunology, Immunotherapy: CII
Franziska Hampel, Peter Hallscheidt, Christof Sohn, Bettina Schlehe, Kerstin A Brocker
AIMS: To perform pelvimetry in nulliparous and primiparous women using 3 Tesla magnetic resonance imaging (3T MRI). METHODS: Twenty-five nulliparous volunteers and 25 primiparous women underwent pelvic 3T MRI within one week after vaginal childbirth in a prospective clinical single-center trial. The pelvimetric parameters interspinous distance (ISD), intertuberous distance (ITD), sagittal outlet (SO), obstetric conjugate (OC), and coccygeal curved length (CCL) were adapted from anthropometric measurements as well as from sonographic and computed tomography-based pelvimetry performed on high-resolution T2-weighted images...
February 21, 2018: Neurourology and Urodynamics
Philip Foerstner, Rida Rehman, Sofia Anastasiadou, Melanie Haffner-Luntzer, Daniela Sinske, Anita Ignatius, Francesco Roselli, Bernd Knoell
Traumatic brain injury (TBI) induces a neuroinflammatory response resulting in astrocyte and microglia activation at the lesion site. This involves up-regulation of neuroinflammatory genes including chemokines and interleukins. However, so far there is lack of knowledge on transcription factors (TFs) modulating this TBI associated gene expression response. Herein we analyzed activating transcription factor 3 (ATF3), a TF encoding a regeneration-associated gene (RAG) predominantly studied in peripheral nervous system (PNS) injury...
February 20, 2018: Journal of Neurotrauma
Sahar Y Al-Okbi, Doha A Mohamed, Thanaa E Hamed, Amr E Edris, Karem Fouda
The aim of the present research was to investigate the effect of fish oil, crude Nigella sative oil and combined fish oil/Nigella sative volatile oil as hepato-regenerative and renal protective supplements. The oils were administered as emulsions to rat model with liver injury induced by CCl4 . Plasma activities of transaminases (AST and ALT) were evaluated as liver function indicators, while plasma creatinine and urea and creatinine clearance were determined as markers of kidney function. Plasma malondialdehyde (MDA), nitrite (NO) and tumor necrosis factor-α (TNF-α) were estimated to assess the exposure to oxidative stress and subsequent inflammation...
February 19, 2018: Journal of Oleo Science
Scholastica Onyebuchi Anadozie, Jacob Ayodele Akinyemi, Shadrach Agunbiade, Basiru Olaitan Ajiboye, Olusola Bolaji Adewale
Bryophyllum pinnatum (B. pinnatum) (Lam.) Oken is used in tropical Africa for the treatment of several diseases such as kidney and urinary disorders. This study was aimed to evaluate the effect of B. pinnatum on arginase II activity and its prevention against renal oxidative damage occasioned by CCl4 in rats. Rats were randomly divided into six groups; group I served as the control, group II served as carbon tetrachloride (CCl4 ) intoxicated group, group III-V animals were pre-treated with silymarin (25 mg/kg body weight), 25 mg/kg body weight aqueous extracts of Bryophyllum pinnatum (AEBP) and 50 mg/kg body weight AEBP, respectively, for 14 days, followed by a single injection of CCl4...
February 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Kelvin K W To, Lu Lu, Carol H Y Fong, Alan K L Wu, Ka-Yi Mok, Cyril C Y Yip, Yi-Hong Ke, Kong-Hung Sze, Susanna K P Lau, Ivan F N Hung, Kwok-Yung Yuen
OBJECTIVES: We assessed the immunological response of hospitalized adult patients with rhinovirus infection, including critically-ill patients. METHODS: The differential white blood cell (WBC) count and the levels of 29 plasma cytokines/chemokines were compared between 50 adult hospitalized patients with rhinovirus infection and 100 age-matched controls with influenza virus infection. RESULTS: The demographics and comorbidities were similar between rhinovirus and influenza patients, but severe disease was more common for the rhinovirus cohort...
February 15, 2018: Journal of Infection
Meng-Bo Hu, Hua Xu, Wen-Hui Zhu, Pei-De Bai, Ji-Meng Hu, Tian Yang, Hao-Wen Jiang, Qiang Ding
High-fat diet (HFD) -induced obesity is associated with more aggressive and lethal prostate cancer (PCa) in males, although the exact underlying mechanisms remain unclear. In the present study, transgenic adenocarcinoma of mouse prostate (TRAMP) models fed on an HFD (40% fat) or a control diet (CD; 16% fat) were generated, and cancer differentiation, local invasion and metastasis were compared at 20, 24 and 28 weeks. Mouse sera from each group were collected, and adipokines and cytokines were measured using multiplex immunoassays...
February 2018: Oncology Letters
Youwen Cao, Jinlin He, Jie Liu, Mingzu Zhang, Peihong Ni
To improve the therapeutic efficacy and circulation stability in vivo, we synthesized a new kind of drug delivery carrier based on folic acid-conjugated polyphosphoester via the combined reactions of Michael addition polymerization and esteri-fication. The produced amphiphilic polymer, abbreviated as P(EAEP-AP)-LA-FA, could self-assemble into nanoparticles (NPs) with core-shell structure in water and reversible core cross-linked by lipoyl groups. Using the core cross-linked FA-conjugated nanoparticles (CCL-FA NPs) to encapsulate hydrophobic anticancer drug doxorubicin (DOX), we studied the stability of NPs, in vitro drug release, cellular uptake and targeting intracellular release, compared with both uncross-linked FA-conjugated nanoparticles (UCL-FA NPs) and core cross-linked nanoparticles without FA-conjugated (CCL NPs)...
February 12, 2018: ACS Applied Materials & Interfaces
Bei Zhong, Yu Li, Xiaodong Liu, Dongsheng Wang
The present study aimed to determine the expression of mast cells, C-C motif chemokine ligand 2 (CCL-2) and C-C motif chemokine receptor 2 (CCR2) in gastric cancer tumor tissue; and the association of mast cells with the proliferation, migration, invasion and apoptosis of gastric cancer cells. In addition, whether the stem cell factor (SCF)/c-Kit pathway was associated with the secretion of CCL-2 by gastric cancer cells was explored. Flow cytometry analysis and immunohistochemistry were used to observe the relative number of mast cells, and reverse transcription-quantitative polymerase chain reaction and western blot analysis were utilized to determine the expression of CCL-2 and CCR2 mRNA and protein...
January 2018: Oncology Letters
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