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https://www.readbyqxmd.com/read/28654245/effects-of-in-utero-pfos-exposure-on-transcriptome-lipidome-and-function-of-mouse-testis
#1
Keng Po Lai, Jetty Chung-Yung Lee, Hin Ting Wan, Jing Woei Li, Aman Yi-Man Wong, Ting-Fung Chan, Camille Oger, Jean-Marie Galano, Thierry Durand, Kin Sum Leung, Cherry C Leung, Rong Li, Chris Kong-Chu Wong
Transcriptomic and LC-MS/MS-based targeted lipidomic analyses were conducted to identify the effects of in utero PFOS exposure on neonatal testes and its relation to testicular dysfunction in adult offspring. Pregnant mice were orally administered 0.3 and 3 µg PFOS/g body weight until term. Neonatal testes (P1) were collected for the detection of PFOS, and were subjected to omics study. Integrated pathway analyses using DAVID, KEGG and IPA underlined the effects of PFOS exposure on lipid metabolism, oxidative stress and cell junction signaling in testes...
June 27, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28651702/effect-of-dha-supplementation-on-oxylipin-levels-in-plasma-and-immune-cell-stimulated-blood
#2
Jan Philipp Schuchardt, Annika I Ostermann, Lisa Stork, Sabrina Fritzsch, Heike Kohrs, Theresa Greupner, Andreas Hahn, Nils Helge Schebb
INTRODUCTION: EPA and DHA cause different physiological effects, which are in many cases mediated via their oxidative metabolites (oxylipins). However, metabolism studies investigating the effect of either EPA or DHA on comprehensive oxylipin patterns are lacking. MATERIAL AND METHODS: The short and long term (1, 3, 6, and 12 week) effect of 1076mg/d DHA (free of EPA) on free (unesterified) oxylipin concentrations in plasma and lipopolysacharid (LPS) stimulated blood of 12 healthy men (mean age 25...
June 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28651009/interleukin-4-receptor-alpha-is-still-required-after-th2-polarization-for-the-maintenance-and-the-recall-of-protective-immunity-to-nematode-infection
#3
Justin Komguep Nono, Hlumani Ndlovu, Nada Abdel Aziz, Thabo Mpotje, Lerato Hlaka, Frank Brombacher
There is currently no vaccine against parasitic nematodes and the knowledge on the mechanisms by which protective immunity against this class of parasites is achieved is continuously expanding. Nematode parasites trigger a host protective type 2 immune response via interleukin-4 receptor alpha (IL-4Rα). Despite this central role, it is not known whether IL-4Rα has a role in maintaining host type 2 immune responses following polarization. To determine the role of IL-4Rα after polarization, we used a recently established strain of rosaCreERT2-/+IL-4Rα-/Lox mice where il4rα gene deletion can be temporally controlled...
June 26, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28645539/gli1-labeled-adult-mesenchymal-stem-progenitor-cells-and-hedgehog-signaling-contribute-to-endochondral-heterotopic-ossification
#4
Chen Kan, Lijun Chen, Yangyang Hu, Na Ding, Yuyun Li, Tammy L McGuire, Haimei Lu, John A Kessler, Lixin Kan
Heterotopic ossification (HO), acquired or hereditary, endochondral or intramembranous, is the formation of true bone outside the normal skeleton. Since perivascular Gli1+ progenitors contribute to injury induced organ fibrosis, and CD133 is expressed by a variety of populations adult stem cells, this study utilized Cre-lox based genetic lineage tracing to test the contribution to endochondral HO of adult stem/progenitor cells that expressed either Gli1 or CD133. We found that both lineages contributed broadly to different normal tissues with distinct patterns, but that only Gli1-creERT labeled stem/progenitor cells contributed to all stages of endochondral HO in a BMP dependent, injury induced, transgenic mouse model...
June 20, 2017: Bone
https://www.readbyqxmd.com/read/28642826/elucidation-of-the-role-of-lectin-like-oxldl-receptor-1-in-the-metabolic-responses-of-macrophages-to-human-oxldl
#5
Danielle W Kimmel, William P Dole, David E Cliffel
Atherogenesis is the narrowing of arteries due to plaque build-up that results in cardiovascular disease that can lead to death. The macrophage lectin-like oxidized LDL receptor-1 (LOX-1), also called the oxidized low-density lipoprotein receptor 1 (OLR1), is currently thought to aid in atherosclerotic disease progression; therefore metabolic studies have potential to both provide mechanistic validation for the role of LOX-1 in disease progression and provide valuable information regarding biomarker strategies and clinical imaging...
2017: Journal of Lipids
https://www.readbyqxmd.com/read/28641662/-effect-of-pdk1-on-neutrophil-differentiation
#6
Wei-Li Wang, Le Wang, Tian-Yuan Hu, Cong Li, Wei-Ping Yuan
OBJECTIVE: To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio, number and differentiation of neutrophil. METHODS: The PDK1 expression level of Vav-Cre;PDK1(lox/lox) mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis. RESULTS: The RNA expression level of bone marrow cells of Vav-Cre;PDK1(lox/lox) mouse was dramatically lower than that of the control mouse...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28640638/enzyme-mediated-conversion-of-fad-to-8-formyl-fad-in-formate-oxidase-results-in-modified-cofactor-with-enhanced-catalytic-properties
#7
John M Robbins, Michael G Souffrant, Donald Hamelberg, Giovanni Gadda, Andreas S Bommarius
Flavins, including flavin adenine dinucleotide (FAD), are fundamental catalytic cofactors responsible for the redox functionality of a diverse set of proteins. Alternatively, modified flavin analogues are rarely found in nature as their incorporation typically results in inactivation of flavoproteins, thus leading to the disruption of important cellular pathways. Here, we report that the fungal flavoenzyme formate oxidase (FOX) catalyzes the slow conversion of non-covalently bound FAD to 8-formyl FAD (8-fFAD), and that this conversion results in a nearly 10-fold increase in formate oxidase activity...
June 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28633105/effects-of-triacontanol-on-ascorbate-glutathione-cycle-in-brassica-napus-l-exposed-to-cadmium-induced-oxidative-stress
#8
Elham Asadi Karam, Viviana Maresca, Sergio Sorbo, Batool Keramat, Adriana Basile
The ability of exogenous triacontanol (TRIA), a plant growth regulator, to reduce Cd toxicity was studied in canola (Brassica napus L.) plants. The following biological parameters were examined in canola seedlings to investigate TRIA-induced tolerance to Cd toxicity: seedling growth, chlorophyll damage and antioxidant response. In particular, TRIA application reduced Cd-induced oxidative damage, as shown by reduction of ROS content, lipoxygenase (LOX) activity and lipid peroxidation level. TRIA pretreatment increased non-enzymatic antioxidant contents (ascorbate, AsA, glutathione and GSH), phytochelatin content (PCs) and activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), monodehydroascorbate reductase (MDHAR), dehydro ascorbate reductase (DHAR), and glutathione reductase (GR), so reducing the oxidative stress...
June 20, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28627266/anti-inflammatory-effect-of-a-powder-formulation-of-coconut-inflorescence-sap-against-ox-ldl-induced-inflammatory-responses-in-human-peripheral-blood-mononuclear-cells-by-modulating-tlr-nf-%C3%B2-b-signaling-pathway
#9
Ratheesh M, Svenia P Jose, Asha S, Sandya S, Girish Kumar B, Krishnakumar Im
An inflammatory response induced by oxidized low density lipoprotein (ox-LDL) was reported to play an important role in the pathogenesis of atherosclerosis. The purpose of this study was to explore the anti-inflammatory effect of a novel powder formulation of coconut inflorescence sap (CSP) against ox-LDL induced inflammatory responses in human peripheral blood mononuclear cells (hPBMCs). The hPBMCs were isolated from healthy human volunteers and cultured in collagen coated plates at 37°C. The cells were grouped as Group I (Control), Group II (ox-LDL treated) and Group III (ox-LDL+CSP treated)...
June 19, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/28625237/-study-on-mechanisms-and-myocardial-protective-effect-of-qishen-yiqi-dropping-pills-on-rats-with-myocardial-infarction
#10
Quan Yang, Yunshan Cao
OBJECTIVE: To approach the mechanisms and myocardial protective effect of Qishen Yiqi dropping pills on rats with myocardial infarction. METHODS: Sixty clean healthy male Sprague-Dawley (SD) rats were randomly divided into sham operation group, model group and observation group (each n = 20). The rat model of acute myocardial infarction (AMI) was established by ligation of left anterior descent (LAD) branch of coronary artery. After modeling, the rats in observation group were given 0...
June 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28624448/identification-of-functional-hypoxia-inducible-factor-response-elements-in-the-human-lysyl-oxidase-gene-promoter
#11
Victoria Wang, David A Davis, Robert Yarchoan
Human lysyl oxidase (LOX) is a hypoxia-responsive gene whose product catalyzes collagen crosslinking and is thought to be important in cancer metastasis and osteoarthritis. We previously demonstrated that LOX was upregulated by hypoxia inducible factor 2 (HIF-2) more strongly than hypoxia inducible 1 (HIF-1). We further investigated the response of the LOX gene and LOX promoter to HIFs. LOX mRNA, measured by real time reverse transcriptase-PCR, was strongly up-regulated (almost 40-fold), by transfection of HEK-293T cells with a plasmid encoding the HIF-2α subunit of HIF-2, but only three-fold by a plasmid encoding HIF-1α...
June 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28624291/vascular-lysyl-oxidase-over-expression-alters-extracellular-matrix-structure-and-induces-oxidative-stress
#12
Saray Varona, Ana B García-Redondo, Jose Martínez-González, Mercedes Salaices, Ana M Briones, Cristina Rodríguez
INTRODUCTION: Lysyl oxidase (LOX) participates in the assembly of collagen and elastin fibres. The impact of vascular LOX over-expression on extracellular matrix (ECM) structure and its contribution to oxidative stress has been analysed. METHODS: Studies were conducted on mice over-expressing LOX (Tg), specifically in smooth muscle cells (VSMC). Gene expression was assessed by real-time PCR analysis. Sirius Red staining, H2O2 production and NADPH oxidase activity were analysed in different vascular beds...
June 14, 2017: Clínica e Investigación en Arteriosclerosis
https://www.readbyqxmd.com/read/28623868/ozone-induced-foliar-damage-and-release-of-stress-volatiles-is-highly-dependent-on-stomatal-openness-and-priming-by-low-level-ozone-exposure-in-phaseolus-vulgaris
#13
Shuai Li, Peter C Harley, Ülo Niinemets
Acute ozone exposure triggers major emissions of volatile organic compounds (VOC), but quantitatively, it is unclear how different ozone doses alter the start and the total amount of these emissions, and the induction rate of different stress volatiles. It is also unclear whether priming (i.e., pre-exposure to lower O3 concentrations) can modify the magnitude and kinetics of volatile emissions. We investigated photosynthetic characteristics and VOC emissions in Phaseolus vulgaris following acute ozone exposure (600 nmol mol(-1) for 30 min) under illumination and in darkness and after priming with 200 nmol mol(-1) O3 for 30 min...
June 17, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28611577/the-anti-inflammatory-effects-of-lipoxygenase-and-cyclo-oxygenase-inhibitors-in-inflammation-induced-human-fetal-glia-cells-and-the-a%C3%AE-degradation-capacity-of-human-fetal-astrocytes-in-an-ex-vivo-assay
#14
Rea Pihlaja, Merja Haaparanta-Solin, Juha O Rinne
Chronic inflammation is a common phenomenon present in the background of multiple neurodegenerative diseases, including Alzheimer's disease (AD). The arachidonic acid pathway overproduces proinflammatory eicosanoids during these states and glial cells in the brain gradually lose their vital functions of protecting and supporting neurons. In this study, the role of different key enzymes of the eicosanoid pathway mediating inflammatory responses was examined in vitro and ex vivo using human fetal glial cells...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28611151/fate-mapping-neurons-and-glia-derived-from-dbx1-expressing-progenitors-in-mouse-preb%C3%A3-tzinger-complex
#15
Andrew Kottick, Caroline A Martin, Christopher A Del Negro
The brainstem preBötzinger complex (preBötC) generates the inspiratory breathing rhythm, and its core rhythmogenic interneurons are derived from Dbx1-expressing progenitors. To study the neural bases of breathing, tamoxifen-inducible Cre-driver mice and Cre-dependent reporters are used to identify, record, and perturb Dbx1 preBötC neurons. However, the relationship between tamoxifen administration and reporter protein expression in preBötC neurons and glia has not been quantified. To address this problem, we crossed mice that express tamoxifen-inducible Cre recombinase under the control of the Dbx1 gene (Dbx1(Cre)(ERT)(2)) with Cre-dependent fluorescent reporter mice (Rosa26(tdTomato)), administered tamoxifen at different times during development, and analyzed tdTomato expression in the preBötC of their offspring...
June 2017: Physiological Reports
https://www.readbyqxmd.com/read/28601780/sirt1-inhibition-causes-oxidative-stress-and-inflammation-in-patients-with-coronary-artery-disease
#16
Shih-Hung Chan, Ching-Hsia Hung, Jhih-Yuan Shih, Pei-Ming Chu, Yung-Hsin Cheng, Huei-Chen Lin, Kun-Ling Tsai
Coronary artery disease (CAD) is the primary critical cardiovascular event. Endothelial cell and monocyte dysfunction with subsequent extravagant inflammation are the main causes of vessel damage in CAD. Thus, strategies that repress cell death and manage unsuitable pro-inflammatory responses in CAD are potential therapeutic strategies for improving the clinical prognosis of patients with CAD. SIRT1 (Sirtuin 1) plays an important role in regulating cellular physiological processes. SIRT1 is also thought to protect the cardiovascular system by means of its antioxidant, anti-inflammation and anti-apoptosis activities...
June 2, 2017: Redox Biology
https://www.readbyqxmd.com/read/28599988/pharmacological-evaluation-and-molecular-docking-of-new-di-tert-butylphenol-compound-lqfm-091-a-new-dual-5-lox-cox-inhibitor
#17
Roberta Campos Lino, Daiany Priscila Bueno da Silva, Iziara Ferreira Florentino, Dayane Moreira da Silva, José Luís Rodrigues Martins, Daniel da Costa Batista, Karla Carneiro de Siqueira Leite, Bianca Villavicencio, Géssica A Vasconcelos, Andreia Luiza Pereira Silva, Renato Ivan de Ávila, Hugo Verli, Marize Campos Valadares, Eric de Souza Gil, Boniek G Vaz, Luciano M Lião, Ricardo Menegatti, Elson Alves Costa
Dual 5-LOX/COX inhibitors are potential new dual drugs to treat inflammatory conditions. This research aimed to design, synthesis and to evaluate the anti-inflammatory and antinociceptive effects of the new compound, which is derived from nimesulide and darbufelone lead compounds. The new dual inhibitor 5-LOX/COX has the possible advantage of gastrointestinal safety. A voltammetric experiment was conducted to observe the drug's antioxidative effect. A formalin test, a hot plate test and carrageenan-induced mechanical hyperalgesia were employed to evaluate the analgesic nature of LQFM-091...
June 10, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28596376/cre-lox-studies-identify-resident-macrophages-as-the-major-source-of-circulating-coagulation-factor-xiii-a
#18
Cora M L Beckers, Kingsley R Simpson, Kathryn J Griffin, Jane M Brown, Lih T Cheah, Kerrie A Smith, Jean Vacher, Paul A Cordell, Mark T Kearney, Peter J Grant, Richard J Pease
OBJECTIVE: To establish the cellular source of plasma factor (F)XIII-A. APPROACH AND RESULTS: A novel mouse floxed for the F13a1 gene, FXIII-A(flox/flox) (Flox), was crossed with myeloid- and platelet-cre-expressing mice, and cellular FXIII-A mRNA expression and plasma and platelet FXIII-A levels were measured. The platelet factor 4-cre.Flox cross abolished platelet FXIII-A and reduced plasma FXIII-A to 23±3% (P<0.001). However, the effect of platelet factor 4-cre on plasma FXIII-A was exerted outside of the megakaryocyte lineage because plasma FXIII-A was not reduced in the Mpl(-/-) mouse, despite marked thrombocytopenia...
June 8, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28595908/association-of-angiotensin-ii-receptor-1-and-lectin-like-oxidized-low-density-lipoprotein-receptor-1-mediates-the-cardiac-hypertrophy%C3%A2-induced-by-oxidized-low-density-lipoprotein
#19
Yunzi Zhao, Yuqiu Li, Xiaohui Ning, Keping Chen, Shu Zhang
To date the molecular mechanism of cardiac hypertrophy has not been completely elucidated. Since oxidized low-density lipoprotein (ox-LDL) is considered a risk marker for early ventricular remodeling, we speculated that ox-LDL may be related to cardiac hypertrophy. We observed the significantly upregulation of plasma ox-LDL and hypertrophic responses, such as cardiomyocyte size and specific gene expressions in Apo E(-/-) mice fed with high fat diet, accompanied by the upregulation of AT1-R and lectin-like oxidized low-density protein receptor 1 (LOX-1)...
June 5, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28595877/lipoxygenase-inhibitors-protect-acute-lymphoblastic-leukemia-cells-from-ferroptotic-cell-death
#20
Lukas Probst, Jasmin Dächert, Barbara Schenk, Simone Fulda
Ferroptosis has recently been identified as a mode of programmed cell death. However, little is yet known about the signaling mechanisms. Here, we report that lipoxygenases (LOX) contribute to the regulation of RSL3-induced ferroptosis in acute lymphoblastic leukemia (ALL) cells. We show that the glutathione (GSH) peroxidase 4 (GPX4) inhibitor RSL3 triggers lipid peroxidation, production of reactive oxygen species (ROS) and cell death in ALL cells. All these events are impeded in the presence of Ferrostatin-1 (Fer-1), a small-molecule inhibitor of lipid peroxidation...
June 5, 2017: Biochemical Pharmacology
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