Miguel Fernández, Juan J Alvear-Arias, Emerson M Carmona, Christian Carrillo, Antonio Pena-Pichicoi, Erick O Hernandez-Ochoa, Alan Neely, Osvaldo Alvarez, Ramon Latorre, Jose A Garate, Carlos Gonzalez
The majority of voltage-gated ion channels contain a defined voltage-sensing domain and a pore domain composed of highly conserved amino acid residues that confer electrical excitability via electromechanical coupling. In this sense, the voltage-gated proton channel (Hv1) is a unique protein in that voltage-sensing, proton permeation and pH-dependent modulation involve the same structural region. In fact, these processes synergistically work in concert, and it is difficult to separate them. To investigate the process of Hv1 voltage sensor trapping, we follow voltage-sensor movements directly by leveraging mutations that enable the measurement of Hv1 channel gating currents...
December 28, 2023: International Journal of Molecular Sciences