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Acid-sensing ion channels

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https://www.readbyqxmd.com/read/27896621/distinct-roles-of-asic3-and-trpv1-receptors-in-electroacupuncture-induced-segmental-and-systemic-analgesia
#1
Juanjuan Xin, Yangshuai Su, Zhaokun Yang, Wei He, Hong Shi, Xiaoyu Wang, Ling Hu, Xiaochun Yu, Xianghong Jing, Bing Zhu
Previous studies have demonstrated the effects of different afferent fibers on electroacupuncture (EA)-induced analgesia. However, contributions of functional receptors expressed on afferent fibers to the EA analgesia remain unclear. This study investigates the roles of acid-sensing ion channel 3 (ASIC3) and transient receptor potential vanilloid 1 (TRPV1) receptors in EA-induced segmental and systemic analgesia. Effects of EA at acupoint ST36 with different intensities on the C-fiber reflex and mechanical and thermal pain thresholds were measured among the ASIC3(-/-), TRPV1(-/-), and C57BL/6 mice...
November 28, 2016: Frontiers of Medicine
https://www.readbyqxmd.com/read/27879566/the-effect-of-an-acid-sensing-ion-channels-asics-inhibitor-on-pain-related-behavior-by-nucleus-pulposus-applied-on-the-nerve-root-in-rats
#2
Yoshihiro Kobayashi, Miho Sekiguchi, Shin-Ichi Konno
STUDY DESIGN: Controlled, interventional, animal study. OBJECTIVE: To examine the effect of an inhibitor of acid-sensing ion channel 3 (ASIC3) on pain-related behavior induced by application of the nucleus pulposus (NP) onto the dorsal root ganglion (DRG) in rats. SUMMARY OF BACKGROUND DATA: ASIC3 is associated with acidosis pain in inflamed or ischemic tissues and is expressed in sensory neurons and NP cells. The ASIC3 inhibitor, APETx2, increases the mechanical threshold of pain in models of knee osteoarthritis or postoperative pain...
November 22, 2016: Spine
https://www.readbyqxmd.com/read/27872215/intramolecular-interactions-that-control-voltage-sensitivity-in-the-jshak1-potassium-channel-from-polyorchis-penicillatus
#3
Nazlee Sharmin, Warren J Gallin
Voltage-gated potassium ion (Kv) channel proteins respond to changes in membrane potential by changing the probability of K(+) flux through an ion-selective pore. Kv channels from different paralogous and orthologous families have widely varying V50 values. The voltage-sensing transmembrane helices (S4) of different channels contain 4-7 basic residues that are responsible for transducing changes in transmembrane potential into the energy required to shift the equilibrium between the open- and closed-channel conformations...
November 21, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27853274/acidic-ph-promotes-intervertebral-disc-degeneration-acid-sensing-ion-channel-3-as-a-potential-therapeutic-target
#4
Hamish T J Gilbert, Nathan Hodson, Pauline Baird, Stephen M Richardson, Judith A Hoyland
The aetiology of intervertebral disc (IVD) degeneration remains poorly understood. Painful IVD degeneration is associated with an acidic intradiscal pH but the response of NP cells to this aberrant microenvironmental factor remains to be fully characterised. The aim here was to address the hypothesis that acidic pH, similar to that found in degenerate IVDs, leads to the altered cell/functional phenotype observed during IVD degeneration, and to investigate the involvement of acid-sensing ion channel (ASIC) -3 in the response...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27853254/gastrodin-protects-against-chronic-inflammatory-pain-by-inhibiting-spinal-synaptic-potentiation
#5
Mei-Mei Xiao, Yu-Qi Zhang, Wen-Ting Wang, Wen-Juan Han, Zhen Lin, Rou-Gang Xie, Zhi Cao, Na Lu, San-Jue Hu, Sheng-Xi Wu, Hui Dong, Ceng Luo
Tissue injury is known to produce inflammation and pain. Synaptic potentiation between peripheral nociceptors and spinal lamina I neurons has been proposed to serve as a trigger for chronic inflammatory pain. Gastrodin is a main bioactive constituent of the traditional Chinese herbal medicine Gastrodia elata Blume, which has been widely used as an analgesic since ancient times. However, its underlying cellular mechanisms have remained elusive. The present study demonstrated for the first time that gastrodin exhibits an analgesic effect at the spinal level on spontaneous pain, mechanical and thermal pain hypersensitivity induced by peripheral inflammation, which is not dependent on opioid receptors and without tolerance...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27827430/lactate-is-a-potent-inhibitor-of-the-capsaicin-receptor-trpv1
#6
Jeanne de la Roche, Isabella Walther, Waleria Leonow, Axel Hage, Mirjam Eberhardt, Martin Fischer, Peter W Reeh, Susanne Sauer, Andreas Leffler
Tissue ischemia results in an accumulation of lactate and local or systemic lactic acidosis. In nociceptive sensory neurons, lactate was reported to sensitize or activate the transient receptor potential ion channel TRPA1 and acid-sensing ion channels (ASICs). However, it is unclear how lactate modulates the TRPV1 regarded as the main sensor for acidosis in sensory neurons. In this study we investigated the effects of lactate (LA) on recombinant and native TRPV1 channels and on TRPV1-mediated release of neuropeptides from mouse nerves...
November 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27820848/acid-sensing-ion-channel-1a-contributes-to-airway-hyperreactivity-in-mice
#7
Leah R Reznikov, David K Meyerholz, Ryan J Adam, Mahmoud Abou Alaiwa, Omar Jaffer, Andrew S Michalski, Linda S Powers, Margaret P Price, David A Stoltz, Michael J Welsh
Neurons innervating the airways contribute to airway hyperreactivity (AHR), a hallmark feature of asthma. Several observations suggested that acid-sensing ion channels (ASICs), neuronal cation channels activated by protons, might contribute to AHR. For example, ASICs are found in vagal sensory neurons that innervate airways, and asthmatic airways can become acidic. Moreover, airway acidification activates ASIC currents and depolarizes neurons innervating airways. We found ASIC1a protein in vagal ganglia neurons, but not airway epithelium or smooth muscle...
2016: PloS One
https://www.readbyqxmd.com/read/27820820/asic1a-deficient-mice-show-unaltered-neurodegeneration-in-the-subacute-mptp-model-of-parkinson-disease
#8
Daniel Komnig, Silke Imgrund, Arno Reich, Stefan Gründer, Björn H Falkenburger
Inflammation contributes to the death of dopaminergic neurons in Parkinson disease and can be accompanied by acidification of extracellular pH, which may activate acid-sensing ion channels (ASIC). Accordingly, amiloride, a non-selective inhibitor of ASIC, was protective in an acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease. To complement these findings we determined MPTP toxicity in mice deficient for ASIC1a, the most common ASIC isoform in neurons. MPTP was applied i...
2016: PloS One
https://www.readbyqxmd.com/read/27770439/non-proton-ligand-sensing-domain-of-acid-sensing-ion-channel-3-is-required-for-itch-sensation
#9
Zhen Lei, Abdul Sami Shaikh, Wenshuai Zheng, Xiao Yu, Jingui Yu, Jingxin Li
Itch, the unpleasant sensation that evokes a desire to scratch, accompanies numerous skin and nervous system disorders. However, the molecular mechanisms of itch are unclear. Acid sensing ion channel 3 (ASIC3) is a sensor of acidic and primary inflammatory pain. The whole-cell patch-clamp technique was used to determine the effect of Chloroquine (CQ)on ASICs currents in primary sensory neurons or the Chinese hamster ovary (CHO) cells transfected with rat-ASIC1a or- ASIC3. Site-directed mutagenesis of plasmid was performed...
October 22, 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27760555/a-modulatory-role-of-asics-on-gabaergic-synapses-in-rat-hippocampal-cell-cultures
#10
Maksim Storozhuk, Elena Kondratskaya, Lyudmila Nikolaenko, Oleg Krishtal
Rapid acidification occurring during synaptic vesicle release can activate acid-sensing ion channels (ASICs) both on pre- and postsynaptic neurons. In the latter case, a fraction of postsynaptic current would be mediated by cation-selective acid-sensing ion channels. Additionally, in both cases, activation of acid-sensing ion channels could modulate synaptic strength by affecting transmitter release and/or sensitivity of postsynaptic receptors. To address potential involvement of acid-sensing ion channels in mediation/modulation of synaptic transmission at hippocampal GABAergic synapses, we studied effects of three structurally different blockers of acid-sensing ion channels on evoked postsynaptic currents using the patch-clamp technique...
October 19, 2016: Molecular Brain
https://www.readbyqxmd.com/read/27746861/acid-sensing-ion-channel-1a-regulates-the-survival-of-nucleus-pulposus-cells-in-the-acidic-environment-of-degenerated-intervertebral-discs
#11
Feng Cai, Feng Wang, Xin Hong, Xin-Hui Xie, Rui Shi, Zhi-Yang Xie, Xiao-Tao Wu
OBJECTIVES: Activation of acid-sensing ion channel 1a (ASIC1a) is responsible for tissue injury caused by acidosis in nervous systems. But its physiological and pathological roles in nucleus pulposus cells (NPCs) are unclear. The aim of this study is to investigate whether ASIC1a regulates the survival of NPCs in the acidic environment of degenerated discs. MATERIALS AND METHODS: NPCs were isolated and cultured followed by immunofluorescent staining and Western-blot analysis for ASIC1a...
August 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27730452/emerging-targets-for-the-management-of-osteoarthritis-pain
#12
Anne-Marie Malfait, Richard J Miller
Worldwide, osteoarthritis (OA) is one of the leading causes of chronic pain, for which adequate relief is not available. Ongoing peripheral input from the affected joint is a major factor in OA-associated pain. Therefore, this review focuses predominantly on peripheral targets emerging in the preclinical and clinical arena. Nerve growth factor is the most advanced of these targets, and its blockade has shown tremendous promise in clinical trials in knee OA. A number of different types of ion channels, including voltage-gated sodium channels and calcium channels, transient receptor potential channels, and acid-sensing ion channels, are important for neuronal excitability and play a role in pain genesis...
October 12, 2016: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/27725147/corrigendum-to-acid-sensing-ion-channels-and-transient-receptor-potential-ion-channels-in-zebrafish-taste-buds-ann-anat-207c-2016-32-37
#13
M Levanti, B Randazzo, E Viña, G Montalbano, O Garcia-Suarez, A Germanà, J A Vega, F Abbate
No abstract text is available yet for this article.
October 17, 2016: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/27698929/enac-deg-in-tumor-development-and-progression
#14
Cui Liu, Li-Li Zhu, Si-Guang Xu, Hong-Long Ji, Xiu-Min Li
The epithelial Na(+) channel/degenerin (ENaC/DEG) superfamily, including the acid-sensing ion channels (ASICs), is characterized by a high degree of similarity in structure but highly diverse in physiological functions. These ion channels have been shown to be important in several physiological functions of normal epithelial cells, including salt homeostasis, fluid transportation and cell mobility. There is increasing evidence suggesting that ENaC/DEG channels are critically engaged in cancer cell biology, such as proliferation, migration, invasion and apoptosis, playing a role in tumor development and progression...
2016: Journal of Cancer
https://www.readbyqxmd.com/read/27687800/participation-of-peripheral-trpv1-trpv4-trpa1-and-asic-in-a-magnesium-sulfate-induced-local-pain-model-in-rat
#15
Dragana Srebro, Sonja Vučković, Milica Prostran
We previously showed that magnesium sulfate (MS) has systemic antinociceptive and local peripheral pronociceptive effects. The role of transient receptor potential (TRP) channels and acid-sensing ion channels (ASICs) in the mechanism of action of MS has not been investigated in detail. The aim of this study was to explore the participation of TRP channels in the pronociceptive action of MS in rats after its intraplantar injection. The paw withdrawal threshold (PWT) to mechanical stimuli was measured by the electronic von Frey test...
December 17, 2016: Neuroscience
https://www.readbyqxmd.com/read/27676696/nerve-growth-factor-release-from-the-urothelium-increases-via-activation-of-bladder-c-fiber-in-rats-with-cerebral-infarction
#16
Ryusei Yokokawa, Hironobu Akino, Hideaki Ito, Xinmin Zha, Osamu Yokoyama
AIMS: There are some reports that bladder C-fibers are partially involved in detrusor overactivity in patients with brain lesions. We investigated the contribution of bladder C-fiber to decreased bladder capacity in rats with cerebral infarction. METHODS: Cerebral infarction was induced under halothane anesthesia by left middle cerebral artery occlusion with 4-0 nylon thread in female Sprague-Dawley rats. Intramural amounts of ATP and prostaglandin E2 , in vivo and in vitro ATP, NGF, and prostaglandin E2 release from the distended bladder urothelium, and changes in mRNA expressions of sensor molecules and receptors were monitored 6 h after the occlusion...
September 27, 2016: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/27638213/low-cost-functional-plasticity-of-trpv1-supports-heat-tolerance-in-squirrels-and-camels
#17
Willem J Laursen, Eve R Schneider, Dana K Merriman, Sviatoslav N Bagriantsev, Elena O Gracheva
The ability to sense heat is crucial for survival. Increased heat tolerance may prove beneficial by conferring the ability to inhabit otherwise prohibitive ecological niches. This phenomenon is widespread and is found in both large and small animals. For example, ground squirrels and camels can tolerate temperatures more than 40 °C better than many other mammalian species, yet a molecular mechanism subserving this ability is unclear. Transient receptor potential vanilloid 1 (TRPV1) is a polymodal ion channel involved in the detection of noxious thermal and chemical stimuli by primary afferents of the somatosensory system...
October 4, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27634419/the-interaction-between-ngf-induced-hyperalgesia-and-acid-provoked-pain-in-the-infrapatellar-fat-pad-and-tibialis-anterior-muscle-of-healthy-volunteers
#18
T K Munkholm, L Arendt-Nielsen
BACKGROUND: Tissue pH is lowered in inflamed tissues, and the increased proton concentration activates acid-sensing ion channels (ASICs), contributing to pain and hyperalgesia. ASICs can be upregulated by nerve growth factor (NGF). The aim of this study was to investigate two new human experimental pain models combining NGF- and acid-induced pain in a randomized, controlled, double-blind study. METHODS: In experiment 1, volunteers (N = 16) received an injection of either NGF or isotonic saline in each infrapatellar fat pad (IFP)...
September 15, 2016: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/27617341/design-of-ru-ii-complexes-based-on-anthraimidazoledione-functionalized-terpyridine-ligand-for-improvement-of-room-temperature-luminescence-characteristics-and-recognition-of-selective-anions-experimental-and-dft-td-dft-study
#19
Debiprasad Mondal, Manoranjan Bar, Shruti Mukherjee, Sujoy Baitalik
In this work we report synthesis and characterization of three rigid and linear rodlike monometallic Ru(II) complexes based on a terpyridine ligand tightly connected to 9,10-anthraquinone electron-acceptor unit through phenyl-imidazole spacer. The motivation of designing these complexes is to enhance their excited-state lifetimes at room temperature. Interestingly it is found that all three complexes exhibit luminescence at room temperature with excited-state lifetimes in the range of 1.6-52.8 ns, depending upon the coligand as well as the solvent...
October 3, 2016: Inorganic Chemistry
https://www.readbyqxmd.com/read/27580245/asic-and-enac-type-sodium-channels-conformational-states-and-the-structures-of-the-ion-selectivity-filters
#20
Israel Hanukoglu
The acid-sensing ion channels (ASICs) and epithelial sodium channels (ENaC) are members of a superfamily of channels that play critical roles in mechanosensation, chemosensation, nociception, and regulation of blood volume and pressure. These channels look and function like a tripartite funnel that directs the flow of Na(+) ions into the cytoplasm via the channel pore in the membrane. The subunits that form these channels share a common structure with two transmembrane segments (TM1 and TM2) and a large extracellular part...
August 31, 2016: FEBS Journal
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