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https://www.readbyqxmd.com/read/28822283/assessment-of-integrated-process-based-on-autohydrolysis-and-robust-delignification-process-for-enzymatic-saccharification-of-bamboo
#1
Tian-Ying Chen, Jia-Long Wen, Bing Wang, Han-Min Wang, Chuan-Fu Liu, Run-Cang Sun
In this study, bamboo (Phyllostachys pubescens) was successfully deconstructed using an integrated process (autohydrolysis and subsequent delignification). Xylooligosaccharides, high-purity lignin, and digestible substrates for producing glucose can be consecutively collected during the integrated process. The structural change and fate of lignin during autohydrolysis process was deeply investigated. Additionally, the structural characteristics and active functional groups of the lignin fractions obtained by these delignification processes were thoroughly investigated by NMR (2D-HSQC and (31)P NMR) and GPC techniques...
August 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28808883/removal-of-water-soluble-extractives-improves-the-enzymatic-digestibility-of-steam-pretreated-softwood-barks
#2
Balázs Frankó, Karin Carlqvist, Mats Galbe, Gunnar Lidén, Ola Wallberg
Softwood bark contains a large amounts of extractives-i.e., soluble lipophilic (such as resin acids) and hydrophilic components (phenolic compounds, stilbenes). The effects of the partial removal of water-soluble extractives before acid-catalyzed steam pretreatment on enzymatic digestibility were assessed for two softwood barks-Norway spruce and Scots pine. A simple hot water extraction step removed more than half of the water-soluble extractives from the barks, which improved the enzymatic digestibility of both steam-pretreated materials...
August 14, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28803154/prospects-recent-advancements-and-challenges-of-different-wastewater-streams-for-microalgal-cultivation
#3
REVIEW
Abhishek Guldhe, Sheena Kumari, Luveshan Ramanna, Prathana Ramsundar, Poonam Singh, Ismail Rawat, Faizal Bux
Microalgae are recognized as one of the most powerful biotechnology platforms for many value added products including biofuels, bioactive compounds, animal and aquaculture feed etc. However, large scale production of microalgal biomass poses challenges due to the requirements of large amounts of water and nutrients for cultivation. Using wastewater for microalgal cultivation has emerged as a potential cost effective strategy for large scale microalgal biomass production. This approach also offers an efficient means to remove nutrients and metals from wastewater making wastewater treatment sustainable and energy efficient...
August 9, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28802003/recombinant-lactococcus-lactis-for-efficient-conversion-of-cellodextrins-into-l-lactic-acid
#4
Chiara Gandini, Loredana Tarraran, Denis Kalemasi, Enrica Pessione, Roberto Mazzoli
Lactic acid bacteria (LAB) are among the most interesting organisms for industrial processes with a long history of application as food starters and biocontrol agents, and an underexploited potential for biorefineries converting biomass into high-value compounds. Lactic acid (LA), their main fermentation product, is among the most requested chemicals owing to its broad range of applications. Notably, LA polymers, i.e., polylactides, have high potential as biodegradable substitutes of fossil-derived plastics...
August 12, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28801256/new-innovative-ecosystems-in-france-to-develop-the-bioeconomy
#5
REVIEW
Thierry Stadler, Jean-Marie Chauvet
The cluster's policy initiated in France in 2005 was a formidable opportunity to boost innovation in the bioeconomy and to develop innovation platforms allowing industrial demonstration and feasibility at technology readiness level (TRL) from 5 to 9. The IAR Cluster (Industries and Agro-Resources), based in the Hauts-de-France and Grand Est regions is completely dedicated to the bioeconomy. IAR's work has contributed to build a complete innovation ecosystem on bioeconomy by opening up relationships between actors along the full value chain...
August 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28780267/coproduction-of-lignin-and-glucose-from-vine-shoots-by-eco-friendly-strategies-toward-the-development-of-an-integrated-biorefinery
#6
Izaskun Dávila, Patricia Gullón, María A Andrés, Jalel Labidi
The objective of this work was to study the suitability of the pretreated vine shoots as a source of lignin and to determine its structural features. The best conditions to achieve the aim of this work were 12% NaOH, 124°C and 105min, as they permitted a removal of 67.7% of the lignin present in the pretreated vine shoots and the obtaining of a solid with a 69.4% of glucan. This delignified solid was subjected to an enzymatic hydrolysis achieving a conversion of glucan to glucose close to 100%. The characterization of lignins extracted from pretreated vine shoots was carried out for the first time and the following techniques were employed: a quantitative acid hydrolysis, HPSEC, TGA, FTIR and Pyrolysis-GC/MS...
July 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28764567/microalgae-a-robust-green-bio-bridge-between-energy-and-environment
#7
Yimin Chen, Changan Xu, Seetharaman Vaidyanathan
Microalgae are a potential candidate for biofuel production and environmental treatment because of their specific characteristics (e.g. fast growth, carbon neutral, and rich lipid accumulations). However, several primary bottlenecks still exist in current technologies, including low biomass conversion efficiency, bio-invasion from the external environment, limited or costly nutrient sources, and high energy and capital input for harvest, and stalling its industrial progression. Coupling biofuel production with environmental treatment renders microalgae a more feasible feedstock...
August 1, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28755283/adsorptive-detoxification-of-fermentation-inhibitors-in-acid-pretreated-liquor-using-functionalized-polymer-designed-by-molecular-simulation
#8
Leena P Devendra, Ashok Pandey
Acid pretreatment is the most common method employed in the lignocellulosic biorefinery leading to the separation of pentose and hexose sugar. The liquor obtained after pretreatment (acid pretreatment liquor or APL) needs to be detoxified prior to fermentation. The aim of this study was to design functional groups on a polymer matrix which are selective in their interaction to inhibitors with little or no specificity to sugars. Molecular modeling was used as a tool to design a suitable adsorbent for selective adsorption of inhibitors from a complex mixture of APL...
July 28, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28747994/lignin-valorization-lignin-nanoparticles-as-high-value-bio-additive-for-multifunctional-nanocomposites
#9
Dong Tian, Jinguang Hu, Jie Bao, Richard P Chandra, Jack N Saddler, Canhui Lu
BACKGROUND: Although conversion of low value but high-volume lignin by-product to its usable form is one of the determinant factors for building an economically feasible integrated lignocellulose biorefinery, it has been challenged by its structural complexity and inhomogeneity. We and others have shown that uniform lignin nanoparticles can be produced from a wide range of technical lignins, despite the varied lignocellulosic biomass and the pretreatment methods/conditions applied. This value-added nanostructure lignin enriched with multifunctional groups can be a promising versatile material platform for various downstream utilizations especially in the emerging nanocomposite fields...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28738503/integration-of-protein-extraction-with-a-stream-of-byproducts-from-marine-macroalgae-a-model-forms-the-basis-for-marine-bioeconomy
#10
Tejal K Gajaria, Poornima Suthar, Ravi S Baghel, Nikunj B Balar, Preeti Sharnagat, Vaibhav A Mantri, C R K Reddy
The present study describes an advanced biorefinery model for marine macroalgae that assumes significant importance in the context of marine bio-economy. The method investigated in this study integrates the extraction of crude proteins with recovery of minerals rich sap, lipids, ulvan and cellulose from fresh biomass of Ulva lactuca. The protein content extracted was 11±2.12% on dry weight basis with recovery efficiency of 68.75±4.01%. The amino acid composition of crude protein fraction showed iso-leucine as the most abundant amino acid with 16...
July 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28737443/fertirrigation-with-sugarcane-vinasse-foreseeing-potential-impacts-on-soil-and-water-resources-through-vinasse-characterization
#11
Lucas T Fuess, Isabella J Rodrigues, Marcelo L Garcia
This paper reports the characterization of the polluting potential of sugarcane vinasse, the main wastewater from ethanol production. Compositional data from vinasse samples collected from sugarcane biorefineries were used to predict negative effects on the soil, water resources and crops potentially associated with fertirrigation, the primary final destination of vinasse in Brazil. High risks of soil salinization were associated with the land disposal of vinasse, as evidenced by the high levels of total dissolved solids (TDS; >4,000 mg L(-1)) and electrical conductivity (>6...
July 24, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28731563/improving-fructose-utilization-and-butanol-production-by-clostridium-acetobutylicum-via-extracellular-redox-potential-regulation-and-intracellular-metabolite-analysis
#12
Li-Jie Chen, You-Duo Wu, Chuang Xue, Feng-Wu Bai
Jerusalem artichoke (JA) can grow well in marginal lands with high biomass yield, and thus is a potential energy crop for biorefinery. The major biomass of JA is from tubers, which contain inulin that can be easily hydrolyzed into a mixture of fructose and glucose, but fructose utilization for producing butanol as an advanced biofuel is poor compared to glucose-based ABE fermentation by Clostridium acetobutylicum. In this article, the impact of extracellular redox potential (ORP) on the process was studied using a mixture of fructose and glucose to simulate the hydrolysate of JA tubers...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731473/ninety-nine-de-novo-assembled-genomes-from-the-moose-alces-alces-rumen-microbiome-provide-new-insights-into-microbial-plant-biomass-degradation
#13
Olov Svartström, Johannes Alneberg, Nicolas Terrapon, Vincent Lombard, Ino de Bruijn, Jonas Malmsten, Ann-Marie Dalin, Emilie El Muller, Pranjul Shah, Paul Wilmes, Bernard Henrissat, Henrik Aspeborg, Anders F Andersson
The moose (Alces alces) is a ruminant that harvests energy from fiber-rich lignocellulose material through carbohydrate-active enzymes (CAZymes) produced by its rumen microbes. We applied shotgun metagenomics to rumen contents from six moose to obtain insights into this microbiome. Following binning, 99 metagenome-assembled genomes (MAGs) belonging to 11 prokaryotic phyla were reconstructed and characterized based on phylogeny and CAZyme profile. The taxonomy of these MAGs reflected the overall composition of the metagenome, with dominance of the phyla Bacteroidetes and Firmicutes...
July 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28721652/effects-of-liquid-hot-water-pretreatment-on-enzymatic-hydrolysis-and-physicochemical-changes-of-corncobs
#14
Saksit Imman, Navadol Laosiripojana, Verawat Champreda
Liquid hot water (LHW) pretreatment is an efficient chemical-free strategy for enhancing enzymatic digestibility of lignocellulosic biomass for conversion to fuels and chemicals in biorefinery. In this study, effects of LHW on removals of hemicelluloses and lignin from corncobs were studied under varying reaction conditions. LHW pretreatment at 160 °C for 10 min promoted the highest levels of hemicellulose solubilization into the liquid phase, resulting into the maximized pentose yield of 58.8% in the liquid and more than 60% removal of lignin from the solid, with 73...
July 19, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28721445/a-two-step-bioprocessing-strategy-in-pentonic-acids-production-from-lignocellulosic-pre-hydrolysate
#15
Xin Zhou, Lu Huang, Yong Xu, Shiyuan Yu
Efficient utilization (over needless disposal) of biorefinery pre-hydrolysate is an economically relevant practice for improving biorefinery financial prospects. The liquid fraction obtained after acid hydrolysis pretreatment of lignocellulosic biomass, called pre-hydrolysate, are predominantly comprised of hemicellulosic carbohydrates. Using a two-step bioprocess, the hexoses were selectively fermented to ethanol by S. cerevisiae to clear the path for Gluconobacter oxydans transformation of pentoses to a high purity pentonic acids solution...
July 18, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28710860/coupling-the-pretreatment-and-hydrolysis-of-lignocellulosic-biomass-by-the-expression-of-beta-xylosidases
#16
Lucía Martín Pérez, Laura Benítez Casanova, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Sandra Gavaldá Martín, Laura Ledesma-García, Noelia Valbuena, Bruno Díez García, Francisco Manuel Reyes-Sosa
Thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years. Although the needs for the reduction of the energy intensity and chemical consumption in the pretreatment step are well known, the reduction of the severity of the process strongly affects the enzymatic hydrolysis yield. To explore the formulation requirements of the well known cellulolytic cocktail from Myceliophthora thermophila on mild pretreated raw materials, this cocktail was tested on steam exploded corn stover without acid impregnation...
July 15, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28709070/ethanol-production-in-a-simultaneous-saccharification-and-fermentation-process-with-interconnected-reactors-employing-hydrodynamic-cavitation-pretreated-sugarcane-bagasse-as-raw-material
#17
Ruly Terán Hilares, João Vitor Ienny, Paulo Franco Marcelino, Muhammad Ajaz Ahmed, Felipe A F Antunes, Silvio Silvério da Silva, Júlio César Dos Santos
In this study, sugarcane bagasse (SCB) pretreated with alkali assisted hydrodynamic cavitation (HC) was investigated for simultaneous saccharification and fermentation (SSF) process for bioethanol production in interconnected column reactors using immobilized Scheffersomyces stipitis NRRL-Y7124. Initially, HC was employed for the evaluation of the reagent used in alkaline pretreatment. Alkalis (NaOH, KOH, Na2CO3, Ca(OH)2) and NaOH recycled black liquor (successive batches) were used and their pretreatment effectiveness was assessed considering the solid composition and its enzymatic digestibility...
July 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28707104/microbial-hydrocarbon-formation-from-biomass
#18
Adrie J J Straathof, Maria C Cuellar
Fossil carbon sources mainly contain hydrocarbons, and these are used on a huge scale as fuel and chemicals. Producing hydrocarbons from biomass instead is receiving increased attention. Achievable yields are modest because oxygen atoms need to be removed from biomass, keeping only the lighter carbon and hydrogen atoms. Microorganisms can perform the required conversions, potentially with high selectivity, using metabolic pathways that often end with decarboxylation. Metabolic and protein engineering are used successfully to achieve hydrocarbon production levels that are relevant in a biorefinery context...
July 14, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28704740/comparative-assessment-of-selected-sugarcane-biorefinery-centered-systems-in-brazil-a-multi-criteria-method-based-on-sustainability-indicators
#19
Edgard Gnansounou, Catarina M Alves, Elia Ruiz Pachón, Pavel Vaskan
This work proposes a new sustainability assessment framework aiming to compare selected options of biorefineries subject to provide the same services to a community. At this end, a concept of biorefinery-centered system helps to develop a joint resources and policy-oriented comparison. When an option of biorefinery cannot provide the given amounts of certain services from its own production, it complements its supply portfolio by purchasing the lacking amounts from complementary and conventional production systems...
July 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28704553/screening-and-characterizing-of-xylanolytic-and-xylose-fermenting-yeasts-isolated-from-the-wood-feeding-termite-reticulitermes-chinensis
#20
Sameh Samir Ali, Jian Wu, Rongrong Xie, Feng Zhou, Jianzhong Sun, Miao Huang
The effective fermentation of xylose remains an intractable challenge in bioethanol industry. The relevant xylanase enzyme is also in a high demand from industry for several biotechnological applications that inevitably in recent times led to many efforts for screening some novel microorganisms for better xylanase production and fermentation performance. Recently, it seems that wood-feeding termites can truly be considered as highly efficient natural bioreactors. The highly specialized gut systems of such insects are not yet fully realized, particularly, in xylose fermentation and xylanase production to advance industrial bioethanol technology as well as industrial applications of xylanases...
2017: PloS One
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