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https://www.readbyqxmd.com/read/28634124/cell-disruption-and-lipid-extraction-for-microalgal-biorefineries-a-review
#1
REVIEW
Soo Youn Lee, Jun Muk Cho, Yong Keun Chang, You-Kwan Oh
The microalgae-based biorefinement process has attracted much attention from academic and industrial researchers attracted to its biofuel, food and nutraceutical applications. In this paper, recent developments in cell-disruption and lipid-extraction methods, focusing on four biotechnologically important microalgal species (namely, Chlamydomonas, Haematococcus, Chlorella, and Nannochloropsis spp.), are reviewed. The structural diversity and rigidity of microalgal cell walls complicate the development of efficient downstream processing methods for cell-disruption and subsequent recovery of intracellular lipid and pigment components...
June 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28631780/tuning-the-photocatalytic-activity-of-bismuth-wolframate-towards-selective-oxidations-for-the-biorefinery-driven-by-solar-light
#2
Rosaria Ciriminna, Riccardo Delisi, Francesco Parrino, Leonardo Palmisano, Mario Pagliaro
The sol-gel entrapment of nanostructured Bi2WO6 enhances the activity and the selectivity of the short-gap semiconductor in the sunlight-driven photo-oxidation of trans-ferulic and trans-cinnamic acid dissolved in water with air as the primary oxidant. Valuable products such as vanillin, benzaldehyde, benzoic acid and vanillic acid are obtained. This provides the proof of concept that photocatalysis could be a promising technology in tomorrow's solar biorefineries.
June 20, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28631029/syngas-biorefinery-and-syngas-utilization
#3
Sashini De Tissera, Michael Köpke, Sean D Simpson, Christopher Humphreys, Nigel P Minton, Peter Dürre
Autotrophic acetogenic bacteria are able to capture carbon (CO or CO2) through gas fermentation, allowing them to grow on a spectrum of waste gases from industry (e.g., steel manufacture and oil refining, coal, and natural gas) and to produce ethanol. They can also consume syn(thesis) gas (CO and H2) made from the gasification of renewable/sustainable resources, such as biomass and domestic/agricultural waste. Acetogenic gas fermentation can, therefore, produce ethanol in any geographic region without competing for food or land...
June 20, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28629103/lignocentric-analysis-of-a-carbohydrate-producing-lignocellulosic-biorefinery-process
#4
Robert H Narron, Qiang Han, Sunkyu Park, Hou-Min Chang, Hasan Jameel
A biologically-based lignocellulosic biorefinery process for obtaining carbohydrates from raw biomass was investigated across six diverse biomasses (three hardwoods & three nonwoods) for the purpose of decoding lignin's influence on sugar production. Acknowledging that lignin could positively alter the economics of an entire process if valorized appropriately, we sought to correlate the chemical properties of lignin within the process to the traditional metrics associated with carbohydrate production-cellulolytic digestibility and total sugar recovery...
June 2, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28625987/metabolic-engineering-of-actinobacillus-succinogenes-provides-insights-into-succinic-acid-biosynthesis
#5
Michael T Guarnieri, Yat-Chen Chou, Davinia Salvachúa, Ali Mohagheghi, Peter C St John, Darren J Peterson, Yannick J Bomble, Gregg T Beckham
Actinobacillus succinogenes, a gram-negative, facultative anaerobe, exhibits the native capacity to convert pentose and hexose sugars to succinic acid (SA) with high yield as a tricarboxylic acid (TCA) cycle intermediate. In addition, A. succinogenes is capnophilic, incorporating CO2 into SA, making this organism an ideal candidate host for conversion of lignocellulosic sugars and CO2 to an emerging commodity bioproduct sourced from renewable feedstocks. In this work, we report the development of facile metabolic engineering capabilities in A...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28624938/synergetic-integration-of-laccase-and-versatile-peroxidase-with-magnetic-silica-microspheres-towards-remediation-of-biorefinery-wastewater
#6
Dhanya Vishnu, Gerard Neeraj, Ramachandran Swaroopini, Ravi Shobana, Vaidyanathan Vinoth Kumar, Hubert Cabana
In this study, a tailor-made biocatalyst consisting of a co-immobilized lignolytic enzyme cascade on multi-functionalized magnetic silica microspheres (MSMS) was developed. Physical adsorption was the most promising strategy for the synthesis of individual immobilized laccase (IL), immobilized versatile peroxidase (IP), as well as co-immobilized laccase (Lac) and versatile peroxidase (VP) with an enzyme activity recovery of about 79, 93, 27, and 27.5%, respectively. Similarly, the biocatalytic load of 116, 183, 23...
June 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28624475/oxidoreductases-on-their-way-to-industrial-biotransformations
#7
REVIEW
Angel T Martínez, Francisco J Ruiz-Dueñas, Susana Camarero, Ana Serrano, Dolores Linde, Henrik Lund, Jesper Vind, Morten Tovborg, Owik M Herold-Majumdar, Martin Hofrichter, Christiane Liers, René Ullrich, Katrin Scheibner, Giovanni Sannia, Alessandra Piscitelli, Cinzia Pezzella, Mehmet E Sener, Sibel Kılıç, Willem J H van Berkel, Victor Guallar, Maria Fátima Lucas, Ralf Zuhse, Roland Ludwig, Frank Hollmann, Elena Fernández-Fueyo, Eric Record, Craig B Faulds, Marta Tortajada, Ib Winckelmann, Jo-Anne Rasmussen, Mirjana Gelo-Pujic, Ana Gutiérrez, José C Del Río, Jorge Rencoret, Miguel Alcalde
Fungi produce heme-containing peroxidases and peroxygenases, flavin-containing oxidases and dehydrogenases, and different copper-containing oxidoreductases involved in the biodegradation of lignin and other recalcitrant compounds. Heme peroxidases comprise the classical ligninolytic peroxidases and the new dye-decolorizing peroxidases, while heme peroxygenases belong to a still largely unexplored superfamily of heme-thiolate proteins. Nevertheless, basidiomycete unspecific peroxygenases have the highest biotechnological interest due to their ability to catalyze a variety of regio- and stereo-selective monooxygenation reactions with H2O2 as the source of oxygen and final electron acceptor...
June 14, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28624245/a-biorefinery-for-nannochloropsis-induction-harvesting-and-extraction-of-epa-rich-oil-and-high-value-protein
#8
REVIEW
Elvis T Chua, Peer M Schenk
Microalgae have been studied as biofactories for almost four decades. Yet, even until today, many aspects of microalgae farming and processing are still considered exploratory because of the uniqueness of each microalgal species. Thus, it is important to develop the entire process of microalgae farming: from culturing to harvesting, and down to extracting the desired high-value products. Based on its rapid growth and high oil productivities, Nannochloropsis sp. is of particular interest to many industries for the production of high-value oil containing omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), but also several other products...
May 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28623567/molecular-identification-and-comparative-evaluation-of-tropical-marine-microalgae-for-biodiesel-production
#9
Sanyo Sabu, I S Bright Singh, Valsamma Joseph
Marine microalgae have emerged as important feedstock for liquid biofuel production. The identification of lipid-rich native microalgal species with high growth rate and optimal fatty acid profile and biodiesel properties is the most challenging step in microalgae-based biodiesel production. In this study, attempts have been made to bio-prospect the biodiesel production potential of marine and brackish water microalgal isolates from the west coast of India. A total of 14 microalgal species were isolated, identified using specific molecular markers and based on the lipid content; seven species with total lipid content above 20% of dry cell weight were selected for assessing biodiesel production potential in terms of lipid and biomass productivities, nile red fluorescence, fatty acid profile and biodiesel properties...
June 16, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28616073/the-transcription-factor-pdr-1-is-a-multi-functional-regulator-and-key-component-of-pectin-deconstruction-and-catabolism-in-neurospora-crassa
#10
Nils Thieme, Vincent W Wu, Axel Dietschmann, Asaf A Salamov, Mei Wang, Jenifer Johnson, Vasanth R Singan, Igor V Grigoriev, N Louise Glass, Chris R Somerville, J Philipp Benz
BACKGROUND: Pectin is an abundant component in many fruit and vegetable wastes and could therefore be an excellent resource for biorefinery, but is currently underutilized. Fungal pectinases already play a crucial role for industrial purposes, such as for foodstuff processing. However, the regulation of pectinase gene expression is still poorly understood. For an optimal utilization of plant biomass for biorefinery and biofuel production, a detailed analysis of the underlying regulatory mechanisms is warranted...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28611790/saccharification-performances-of-miscanthus-at-the-pilot-and-miniaturized-assay-scales-genotype-and-year-variabilities-according-to-the-biomass-composition
#11
Nassim Belmokhtar, Stéphanie Arnoult, Brigitte Chabbert, Jean-Paul Charpentier, Maryse Brancourt-Hulmel
HIGHLIGHTS Biomass production and cell wall composition are differentially impacted by harvesting year and genotypes, influencing then cellulose conversion in miniaturized assay.Using a high-throughput miniaturized and semi-automated method for performing the pretreatment and saccharification steps at laboratory scale allows for the assessment of these factors on the biomass potential for producing bioethanol before moving to the industrial scale. The large genetic diversity of the perennial grass miscanthus makes it suitable for producing cellulosic ethanol in biorefineries...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28610443/valorization-of-potato-peel-a-biorefinery-approach
#12
Pranav D Pathak, Sachin A Mandavgane, Nikhil Manoj Puranik, Swapnil Jaygopal Jambhulkar, Bhaskar D Kulkarni
Potato is the fourth main crop consumed worldwide and is an important constituent in the human diet. Consequently, potato is widely used in food-processing industries. However, these industries generate massive amounts of potato peel (PP) as a by-product, which is usually considered a waste, and is discarded. Interestingly, recent research suggests that PP is a valuable source of bioactive compounds, which can be converted into value-added products. In this study, we review the physicochemical composition and valorization of PP...
June 14, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28609755/single-step-microwave-assisted-hot-water-extraction-of-hemicelluloses-from-selected-lignocellulosic-materials-a-biorefinery-approach
#13
Gezahegn T Mihiretu, Malin Brodin, Annie F Chimphango, Karin Øyaas, Bård H Hoff, Johann F Görgens
The viability of single-step microwave-induced pressurized hot water conditions for co-production of xylan-based biopolymers and bioethanol from aspenwood sawdust and sugarcane trash was investigated. Extraction of hemicelluloses was conducted using microwave-assisted pressurized hot water system. The effects of temperature and time on extraction yield and enzymatic digestibility of resulting solids were determined. Temperatures between 170-200°C for aspenwood and 165-195°C for sugarcane trash; retention times between 8-22min for both feedstocks, were selected for optimization purpose...
May 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28609211/bioprospecting-of-functional-cellulases-from-metagenome-for-second-generation-biofuel-production-a-review
#14
Rameshwar Tiwari, Lata Nain, Nikolaos E Labrou, Pratyoosh Shukla
Second generation biofuel production has been appeared as a sustainable and alternative energy option. The ultimate aim is the development of an industrially feasible and economic conversion process of lignocellulosic biomass into biofuel molecules. Since, cellulose is the most abundant biopolymer and also represented as the photosynthetically fixed form of carbon, the efficient hydrolysis of cellulose is the most important step towards the development of a sustainable biofuel production process. The enzymatic hydrolysis of cellulose by suites of hydrolytic enzymes underlines the importance of cellulase enzyme system in whole hydrolysis process...
June 13, 2017: Critical Reviews in Microbiology
https://www.readbyqxmd.com/read/28604584/lignin-from-micro-to-nanosize-production-methods
#15
REVIEW
Stefan Beisl, Angela Miltner, Anton Friedl
Lignin is the second most abundant biopolymer after cellulose. It has long been obtained as a by-product of cellulose production in pulp and paper production, but had rather low added-value applications. A changing paper market and the emergence of biorefinery projects should generate vast amounts of lignin with the potential of value addition. Nanomaterials offer unique properties and the preparation of lignin nanoparticles and other nanostructures has therefore gained interest as a promising technique to obtain value-added lignin products...
June 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28602076/rapid-generation-of-universal-synthetic-promoters-for-controlled-gene-expression-in-both-gas-fermenting-and-saccharolytic-clostridium-species
#16
Gaohua Yang, Dechen Jia, Lin Jin, Yuqian Jiang, Yong Wang, Weihong Jiang, Yang Gu
Engineering solventogenic clostridia, a group of important industrial microorganisms, to realize their full potential in biorefinery application is still hindered by the absence of plentiful biological parts. Here, we developed an effective approach for rapid generation of a synthetic promoter library in solventogenic clostridia based on a dual-reporter system (catP-lacZ) and a widely used strong thl promoter. The yielded artificial promoters, spanning 2 orders of magnitude, comprised two modular components (the core promoter region and the spacer between RBS and the translation-initiating code), and the strongest promoter had an over 10-fold-higher activity than the original expression part Pthl...
June 16, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28601395/manipulating-environmental-stresses-and-stress-tolerance-of-microalgae-for-enhanced-production-of-lipids-and-value-added-products-a-review
#17
REVIEW
Bailing Chen, Chun Wan, Muhammad Aamer Mehmood, Jo-Shu Chang, Fengwu Bai, Xinqing Zhao
Microalgae have promising potential to produce lipids and a variety of high-value chemicals. Suitable stress conditions such as nitrogen starvation and high salinity could stimulate synthesis and accumulation of lipids and high-value products by microalgae, therefore, various stress-modification strategies were developed to manipulate and optimize cultivation processes to enhance bioproduction efficiency. On the other hand, advancements in omics-based technologies have boosted the research to globally understand microalgal gene regulation under stress conditions, which enable further improvement of production efficiency via genetic engineering...
May 31, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28600944/investigation-of-food-waste-valorization-through-sequential-lactic-acid-fermentative-production-and-anaerobic-digestion-of-fermentation-residues
#18
Francesca Demichelis, Daniel Pleissner, Silvia Fiore, Silvia Mariano, Ivette Michelle Navarro Gutiérrez, Roland Schneider, Joachim Venus
This work concerns the investigation of the sequential production of lactic acid (LA) and biogas from food waste (FW). LA was produced from FW using a Streptococcus sp. strain via simultaneous saccharification and fermentation (SSF) and separate enzymatic hydrolysis and fermentation (SHF). Via SHF a yield of 0.33gLA/gFW (productivity 3.38gLA/L·h) and via SSF 0.29gLA/gFW (productivity 2.08gLA/L·h) was obtained. Fermentation residues and FW underwent anaerobic digestion (3wt% TS). Biogas yields were 0.71, 0...
May 31, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28597311/integrated-process-for-ethanol-biogas-and-edible-filamentous-fungi-based-animal-feed-production-from-dilute-phosphoric-acid-pretreated-wheat-straw
#19
Ramkumar B Nair, Maryam M Kabir, Patrik R Lennartsson, Mohammad J Taherzadeh, Ilona Sárvári Horváth
Integration of wheat straw for a biorefinery-based energy generation process by producing ethanol and biogas together with the production of high-protein fungal biomass (suitable for feed application) was the main focus of the present study. An edible ascomycete fungal strain Neurospora intermedia was used for the ethanol fermentation and subsequent biomass production from dilute phosphoric acid (0.7 to 1.2% w/v) pretreated wheat straw. At optimum pretreatment conditions, an ethanol yield of 84 to 90% of the theoretical maximum, based on glucan content of substrate straw, was observed from fungal fermentation post the enzymatic hydrolysis process...
June 8, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28597218/uncovering-a-new-moral-dilemma-of-economic-optimization-in-biotechnological-processing
#20
Marek Vochozka, Vojtěch Stehel, Anna Maroušková
The trend of emerging biorefineries is to process the harvest as efficiently as possible and without any waste. From the most valuable phytomass, refined medicines, enzymes, dyes and other special reactants are created. Functional foods, food ingredients, oils, alcohol, solvents, plastics, fillers and a wide variety of other chemical products follow. After being treated with nutrient recovery techniques (for fertilizer production), biofuels or soil improvers are produced from the leftovers. Economic optimization algorithms have confirmed that such complex biorefineries can be financially viable only when a high degree of feedstock concentration is included...
June 8, 2017: Science and Engineering Ethics
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