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Xin Zhou, Xuelian Zhou, Xiusheng Tang, Yong Xu
One of the major obstacles in process of lignocellulosic biorefinery is the utilization of pre-hydrolysate from pre-treatment. Although lignocellulosic pre-hydrolysate can serve as an economic starting material for xylonic acid production, the advancement of xylonic acid or xylonate is still limited by further commercial value or applications. In the present study, xylose in the high concentration wheat straw pre-hydrolysate was first in-situ biooxidized to xylonate by Gluconobacter oxydans. To meet the needs of commercialization, crude powdered calcium xylonate was prepared by drying process and calcium xylonate content in the prepared crude product was more than 70%...
April 10, 2018: Bioresource Technology
Anh Duc Nguyen, In Yeub Hwang, Ok Kyung Lee, Donghyuk Kim, Marina G Kalyuzhnaya, Rina Mariyana, Susila Hadiyati, Min Sik Kim, Eun Yeol Lee
Methane is considered a next-generation feedstock, and methanotrophic cell-based biorefinery is attractive for production of a variety of high-value compounds from methane. In this work, we have metabolically engineered Methylomicrobium alcaliphilum 20Z for 2,3-butanediol (2,3-BDO) production from methane. The engineered strain 20Z/pBudK.p, harboring the 2,3-BDO synthesis gene cluster (budABC) from Klebsiella pneumoniae, accumulated 2,3-BDO in methane-fed shake flask cultures with a titer of 35.66mg/L. Expression of the most efficient gene cluster was optimized using selection of promoters, translation initiation rates (TIR), and the combination of 2,3-BDO synthesis genes from different sources...
April 16, 2018: Metabolic Engineering
Andrea Corona, Morten Ambye-Jensen, Giovanna Croxatto Vega, Michael Zwicky Hauschild, Morten Birkved
The Green biorefinery (GBR) is a biorefinery concept that converts fresh biomass into value-added products. The present study combines a Process Flowsheet Simulation (PFS) and Life Cycle Assessment (LCA) to evaluate the technical and environmental performance of different GBR configurations and the cascading utilization of the GBR output. The GBR configurations considered in this study, test alternatives in the three main steps of green-biorefining: fractionation, precipitation, and protein separation. The different cascade utilization alternatives analyse different options for press-pulp utilization, and the LCA results show that the environmental profile of the GBR is highly affected by the utilization of the press-pulp and thus by the choice of conventional product replaced by the press-pulp...
April 13, 2018: Science of the Total Environment
Libo Zhang, Wenxiu Zheng, Ziming Wang, Yubo Ma, Ling Jiang, Tianfu Wang
The aim of this work was to study the degradation of lignin in raw wood via pretreatment with heteropoly acids as substitutes for traditional H2 SO4 in γ-valerolactone/water. By optimizing catalyst concentration, reaction time and temperature, the optimal lignin degradation conditions are obtained (130 °C, 3 h and 20 mM silicotungstic acid). SEM and FTIR measurements demonstrated the efficient lignin degradation ability of HPAs in the GVL/H2 O solvent, with negligible damage to cellulose within the raw wood...
April 5, 2018: Bioresource Technology
Kurt Wagemann, Nils Tippkötter
The terms bioeconomy and biorefineries are used for a variety of processes and developments. This short introduction is intended to provide a delimitation and clarification of the terminology as well as a classification of current biorefinery concepts. The basic process diagrams of the most important biorefinery types are shown.
April 13, 2018: Advances in Biochemical Engineering/biotechnology
Kristina Medhi, Arti Mishra, Indu Shekhar Thakur
We report here the draft genome sequence of Paracoccus denitrificans strain ISTOD1 of 4.9 Mb, isolated from wastewater. It has been identified as a heterotrophic nitrifying and aerobic denitrifying bacterium. Genomic analysis revealed genes related to nitrogen and phosphorus removal, showing that the strain holds potential for bioremediation and biorefinery uses.
April 12, 2018: Genome Announcements
Vera Novy, Bernd Brunner, Bernd Nidetzky
BACKGROUND: Saccharomyces cerevisiae, engineered for L-lactic acid production from glucose and xylose, is a promising production host for lignocellulose-to-lactic acid processes. However, the two principal engineering strategies-pyruvate-to-lactic acid conversion with and without disruption of the competing pyruvate-to-ethanol pathway-have not yet resulted in strains that combine high lactic acid yields (YLA ) and productivities (QLA ) on both sugar substrates. Limitations seemingly arise from a dependency on the carbon source and the aeration conditions, but the underlying effects are poorly understood...
April 11, 2018: Microbial Cell Factories
Haiwei Guo, Daniel M Miles-Barrett, Andrew R Neal, Tao Zhang, Changzhi Li, Nicholas J Westwood
As societal challenges go, the development of efficient biorefineries as a means of reducing our dependence on petroleum refineries is high on the list. One of the core strengths of the petroleum refinery is its ability to produce a huge range of different products using all of the components of the starting material. In contrast, the target of using all the biopolymers present in lignocellulosic biomass is far from realised. Even though our ability to use the carbohydrate-based components has advanced, our plans for lignin lag behind (with the notable exception of vanillin production)...
January 21, 2018: Chemical Science
Xia Wang, Qiaoning He, Yongfu Yang, Jingwen Wang, Katie Haning, Yun Hu, Bo Wu, Mingxiong He, Yaoping Zhang, Jie Bao, Lydia M Contreras, Shihui Yang
Biorefinery of biomass-based biofuels and biochemicals by microorganisms is a competitive alternative of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for commercial production of desirable bioproducts through metabolic engineering. In this review, we summarize the metabolic engineering progress achieved in Z. mobilis to expand its substrate and product ranges as well as to enhance its robustness against stressful conditions such as inhibitory compounds within the lignocellulosic hydrolysates and slurries...
April 5, 2018: Metabolic Engineering
Lucas Tadeu Fuess, Marcelo Loureiro Garcia, Marcelo Zaiat
Sugarcane vinasse has been widely used as a soil fertilizer in the Brazilian sucro-alcohol industry for recycling potassium and water. However, the potential negative effects from long-term soil fertirrigation represent a major drawback regarding this practice, whereas the application of biodigestion represents an efficient method for reducing the polluting organic load and recovering bioenergy from vinasse. Regardless of the predicted use for vinasse, an understanding of the potential of each option is imperative, as the seasonal alterations in the inorganic/organic fractions of vinasse directly affect its management...
April 4, 2018: Science of the Total Environment
Jimmy Anderson Martínez-Ruano, Ashley Sthefanía Caballero-Galván, Daissy Lorena Restrepo-Serna, Carlos Ariel Cardona
Two scenarios for the biogas production using Banana Peel as raw material were evaluated. The first scenario involves the stand-alone production of biogas and the second scenario includes the biogas production together with other products under biorefinery concept. In both scenarios, the influence of the production scale on the process economy was assessed and feasibility limits were defined. For this purpose, the mass and energy balances were established using the software Aspen Plus along with kinetic models reported in the literature...
April 7, 2018: Environmental Science and Pollution Research International
Veera Hämäläinen, Toni Grönroos, Anu Suonpää, Matti Wilhem Heikkilä, Bastiaan Romein, Petri Ihalainen, Sara Malandra, Klara R Birikh
Main hurdles of lignin valorization are its diverse chemical composition, recalcitrance, and poor solubility due to high-molecular weight and branched structure. Controlled fragmentation of lignin could lead to its use in higher value products such as binders, coatings, fillers, etc. Oxidative enzymes (i.e., laccases and peroxidases) have long been proposed as a potentially promising tool in lignin depolymerization. However, their application was limited to ambient pH, where lignin is poorly soluble in water...
2018: Frontiers in Bioengineering and Biotechnology
Kenta Fujii, Yurie Tominaga, Jyumpei Okunaka, Hisashi Yagi, Takashi Ohshiro, Hirokazu Suzuki
Seaweeds are a nonlignocellulosic biomass, but they are often abundant in unique polysaccharides that common microbes can hardly utilize; therefore, polysaccharide degradation is key for the full utilization of seaweed biomass. Here, we isolated 13 thermophiles from seaweed homogenates that had been incubated at high temperature. All of the isolates were Gram-positive and preferentially grew at 60-70 °C. Most formed endospores and were tolerant to seawater salinity. Despite different sources, all isolates were identical regarding 16S rRNA gene sequences and were categorized as Geobacillus thermodenitrificans...
April 4, 2018: Applied Microbiology and Biotechnology
Ryan L Clark, Laura L McGinley, Hugh M Purdy, Travis C Korosh, Jennifer L Reed, Thatcher W Root, Brian F Pfleger
Cyanobacteria are photosynthetic microorganisms whose metabolism can be modified through genetic engineering for production of a wide variety of molecules directly from CO2 , light, and nutrients. Diverse molecules have been produced in small quantities by engineered cyanobacteria to demonstrate the feasibility of photosynthetic biorefineries. Consequently, there is interest in engineering these microorganisms to increase titer and productivity to meet industrial metrics. Unfortunately, differing experimental conditions and cultivation techniques confound comparisons of strains and metabolic engineering strategies...
March 27, 2018: Metabolic Engineering
Giorgia Pagliano, Valeria Ventorino, Antonio Panico, Ida Romano, Alessandro Robertiello, Francesco Pirozzi, Olimpia Pepe
Dairy wastes can be conveniently processed and valorized in a biorefinery value chain since they are abundant, zero-cost and all year round available. For a comprehensive knowledge of the microbial species involved in producing biofuels and valuable intermediates from dairy wastes, the changes in bacterial and archaeal population were evaluated when H2 , CH4 and chemical intermediates were produced. Batch anaerobic tests were conducted with a mixture of mozzarella cheese whey and buttermilk as organic substrate, inoculated with 1% and 3% w/v industrial animal manure pellets...
March 26, 2018: Journal of Environmental Management
Chang Dou, Rick Gustafson, Renata Bura
Background: In the biofuel industry, land productivity is important to feedstock growers and conversion process product yield is important to the biorefinery. The crop productivity, however, may not positively correlate with bioconversion yield. Therefore, it is important to evaluate sugar yield and biomass productivity. In this study, 2-year-old poplar trees harvested in the first coppice cycle, including one low-productivity hybrid and one high-productivity hybrid, were collected from two poplar tree farms...
2018: Biotechnology for Biofuels
Tiago Pinto, Luísa Gouveia, Joana Ortigueira, Ganesh D Saratale, Patrícia Moura
In this work, hydrogen (H2 ) was produced through the fermentation of Spirogyra sp. biomass by Clostridium butyricum DSM 10702. Macronutrient stress was applied to increase the carbohydrate content in Spirogyra, and a 36% (w/w) accumulation of carbohydrates was reached by nitrogen depletion. The use of wet microalga as fermentable substrate was compared with physically and chemically treated biomass for increased carbohydrate solubilisation. The combination of drying, bead beating and mild acid hydrolysis produced a saccharification yield of 90...
March 23, 2018: Journal of Bioscience and Bioengineering
Kiril Manevski, Poul E Lærke, Jørgen E Olesen, Uffe Jørgensen
No abstract text is available yet for this article.
March 23, 2018: Science of the Total Environment
Rudina Bleta, Benedetto Schiavo, Natale Corsaro, Paula Alexandra Costa, Alberto Giaconia, Leonardo Interrante, Eric Monflier, Giuseppe Pipitone, Anne Ponchel, Salvatore Sau, Onofrio Scialdone, Sebastien Tilloy, Alessandro Galia
Hydrothermal liquefaction (HTL) is a promising technology for the production of biocrude oil from microalgae. Although this catalyst-free technology is efficient under high-temperature and high-pressure conditions, the biocrude yield and quality can be further improved by using heterogeneous catalysts. The design of robust catalyst that preserve their performance under hydrothermal conditions will be therefore very important in the development of biorefinery technologies. In this work, we describe two different synthetic routes, i...
March 26, 2018: ACS Applied Materials & Interfaces
Sara Casado López, Mao Peng, Tedros Yonatan Issak, Paul Daly, Ronald P de Vries, Miia R Mäkelä
Fungi can decompose plant biomass to small oligo- and monosaccharides to be used as carbon sources. Some of these small molecules may induce metabolic pathways and production of extracellular enzymes targeted for degradation of plant cell wall polymers. Despite extensive studies in ascomycete fungi, little is known about the nature of inducers for the lignocellulolytic systems of basidiomycetes. In this study, we analyzed six sugars, known to induce expression of lignocellulolytic genes in ascomycetes, for their role as inducer in the basidiomycete white-rot fungus Dichomitus squalens using a transcriptomic approach...
March 23, 2018: Applied and Environmental Microbiology
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