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https://www.readbyqxmd.com/read/28731563/improving-fructose-utilization-and-butanol-production-by-clostridium-acetobutylicum-via-extracellular-redox-potential-regulation-and-intracellular-metabolite-analysis
#1
Li-Jie Chen, You-Duo Wu, Chuang Xue, Feng-Wu Bai
Jerusalem artichoke (JA) can grow well in marginal lands with high biomass yield, and thus is a potential energy crop for biorefinery. The major biomass of JA is from tubers, which contain inulin that can be easily hydrolyzed into a mixture of fructose and glucose, but fructose utilization for producing butanol as an advanced biofuel is poor compared to glucose-based ABE fermentation by Clostridium acetobutylicum. In this article, the impact of extracellular redox potential (ORP) on the process was studied using a mixture of fructose and glucose to simulate the hydrolysate of JA tubers...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731473/ninety-nine-de-novo-assembled-genomes-from-the-moose-alces-alces-rumen-microbiome-provide-new-insights-into-microbial-plant-biomass-degradation
#2
Olov Svartström, Johannes Alneberg, Nicolas Terrapon, Vincent Lombard, Ino de Bruijn, Jonas Malmsten, Ann-Marie Dalin, Emilie El Muller, Pranjul Shah, Paul Wilmes, Bernard Henrissat, Henrik Aspeborg, Anders F Andersson
The moose (Alces alces) is a ruminant that harvests energy from fiber-rich lignocellulose material through carbohydrate-active enzymes (CAZymes) produced by its rumen microbes. We applied shotgun metagenomics to rumen contents from six moose to obtain insights into this microbiome. Following binning, 99 metagenome-assembled genomes (MAGs) belonging to 11 prokaryotic phyla were reconstructed and characterized based on phylogeny and CAZyme profile. The taxonomy of these MAGs reflected the overall composition of the metagenome, with dominance of the phyla Bacteroidetes and Firmicutes...
July 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28721652/effects-of-liquid-hot-water-pretreatment-on-enzymatic-hydrolysis-and-physicochemical-changes-of-corncobs
#3
Saksit Imman, Navadol Laosiripojana, Verawat Champreda
Liquid hot water (LHW) pretreatment is an efficient chemical-free strategy for enhancing enzymatic digestibility of lignocellulosic biomass for conversion to fuels and chemicals in biorefinery. In this study, effects of LHW on removals of hemicelluloses and lignin from corncobs were studied under varying reaction conditions. LHW pretreatment at 160 °C for 10 min promoted the highest levels of hemicellulose solubilization into the liquid phase, resulting into the maximized pentose yield of 58.8% in the liquid and more than 60% removal of lignin from the solid, with 73...
July 19, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28721445/a-two-step-bioprocessing-strategy-in-pentonic-acids-production-from-lignocellulosic-pre-hydrolysate
#4
Xin Zhou, Lu Huang, Yong Xu, Shiyuan Yu
Efficient utilization (over needless disposal) of biorefinery pre-hydrolysate is an economically relevant practice for improving biorefinery financial prospects. The liquid fraction obtained after acid hydrolysis pretreatment of lignocellulosic biomass, called pre-hydrolysate, are predominantly comprised of hemicellulosic carbohydrates. Using a two-step bioprocess, the hexoses were selectively fermented to ethanol by S. cerevisiae to clear the path for Gluconobacter oxydans transformation of pentoses to a high purity pentonic acids solution...
July 18, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28710860/coupling-the-pretreatment-and-hydrolysis-of-lignocellulosic-biomass-by-the-expression-of-beta-xylosidases
#5
Lucía Martín Pérez, Laura Benítez Casanova, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Sandra Gavaldá Martín, Laura Ledesma-García, Noelia Valbuena, Bruno Díez García, Francisco Manuel Reyes-Sosa
Thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years. Although the needs for the reduction of the energy intensity and chemical consumption in the pretreatment step are well known, the reduction of the severity of the process strongly affects the enzymatic hydrolysis yield. To explore the formulation requirements of the well known cellulolytic cocktail from Myceliophthora thermophila on mild pretreated raw materials, this cocktail was tested on steam exploded corn stover without acid impregnation...
July 15, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28709070/ethanol-production-in-a-simultaneous-saccharification-and-fermentation-process-with-interconnected-reactors-employing-hydrodynamic-cavitation-pretreated-sugarcane-bagasse-as-raw-material
#6
Ruly Terán Hilares, João Vitor Ienny, Paulo Franco Marcelino, Muhammad Ajaz Ahmed, Felipe A F Antunes, Silvio Silvério da Silva, Júlio César Dos Santos
In this study, sugarcane bagasse (SCB) pretreated with alkali assisted hydrodynamic cavitation (HC) was investigated for simultaneous saccharification and fermentation (SSF) process for bioethanol production in interconnected column reactors using immobilized Scheffersomyces stipitis NRRL-Y7124. Initially, HC was employed for the evaluation of the reagent used in alkaline pretreatment. Alkalis (NaOH, KOH, Na2CO3, Ca(OH)2) and NaOH recycled black liquor (successive batches) were used and their pretreatment effectiveness was assessed considering the solid composition and its enzymatic digestibility...
July 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28707104/microbial-hydrocarbon-formation-from-biomass
#7
Adrie J J Straathof, Maria C Cuellar
Fossil carbon sources mainly contain hydrocarbons, and these are used on a huge scale as fuel and chemicals. Producing hydrocarbons from biomass instead is receiving increased attention. Achievable yields are modest because oxygen atoms need to be removed from biomass, keeping only the lighter carbon and hydrogen atoms. Microorganisms can perform the required conversions, potentially with high selectivity, using metabolic pathways that often end with decarboxylation. Metabolic and protein engineering are used successfully to achieve hydrocarbon production levels that are relevant in a biorefinery context...
July 14, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28704740/comparative-assessment-of-selected-sugarcane-biorefinery-centered-systems-in-brazil-a-multi-criteria-method-based-on-sustainability-indicators
#8
Edgard Gnansounou, Catarina M Alves, Elia Ruiz Pachón, Pavel Vaskan
This work proposes a new sustainability assessment framework aiming to compare selected options of biorefineries subject to provide the same services to a community. At this end, a concept of biorefinery-centered system helps to develop a joint resources and policy-oriented comparison. When an option of biorefinery cannot provide the given amounts of certain services from its own production, it complements its supply portfolio by purchasing the lacking amounts from complementary and conventional production systems...
July 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28704553/screening-and-characterizing-of-xylanolytic-and-xylose-fermenting-yeasts-isolated-from-the-wood-feeding-termite-reticulitermes-chinensis
#9
Sameh Samir Ali, Jian Wu, Rongrong Xie, Feng Zhou, Jianzhong Sun, Miao Huang
The effective fermentation of xylose remains an intractable challenge in bioethanol industry. The relevant xylanase enzyme is also in a high demand from industry for several biotechnological applications that inevitably in recent times led to many efforts for screening some novel microorganisms for better xylanase production and fermentation performance. Recently, it seems that wood-feeding termites can truly be considered as highly efficient natural bioreactors. The highly specialized gut systems of such insects are not yet fully realized, particularly, in xylose fermentation and xylanase production to advance industrial bioethanol technology as well as industrial applications of xylanases...
2017: PloS One
https://www.readbyqxmd.com/read/28704386/nannochloropsis-oceania-derived-defatted-meal-as-an-alternative-to-fishmeal-in-atlantic-salmon-feeds
#10
Mette Sørensen, Yangyang Gong, Fridrik Bjarnason, Ghana K Vasanth, Dalia Dahle, Mark Huntley, Viswanath Kiron
Defatted microalgal biomass derived from biorefinery can be potential feed ingredients for carnivorous fish. The present study investigated the growth, feed intake:gain and health parameters in Atlantic salmon fed for 84 days with defatted Nannochloropsis oceania as a fishmeal replacer. Fish fed feeds containing the algal biomass (at 10 and 20% inclusion, alga groups) were compared with groups that consumed alga-devoid feeds (control group). The fish that received 20% alga tended to have reduced weight gain and specific growth rate...
2017: PloS One
https://www.readbyqxmd.com/read/28702084/modification-of-the-nanostructure-of-lignocellulose-cell-walls-via-a-non-enzymatic-lignocellulose-deconstruction-system-in-brown-rot-wood-decay-fungi
#11
Barry Goodell, Yuan Zhu, Seong Kim, Kabindra Kafle, Daniel Eastwood, Geoffrey Daniel, Jody Jellison, Makoto Yoshida, Leslie Groom, Sai Venkatesh Pingali, Hugh O'Neill
Wood decayed by brown rot fungi and wood treated with the chelator-mediated Fenton (CMF) reaction, either alone or together with a cellulose enzyme cocktail, was analyzed by small angle neutron scattering (SANS), sum frequency generation (SFG) spectroscopy, Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), atomic force microscopy (AFM), and transmission electron microscopy (TEM). Results showed that the CMF mechanism mimicked brown rot fungal attack for both holocellulose and lignin components of the wood...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28702082/enzymatic-degradation-of-sulfite-pulped-softwoods-and-the-role-of-lpmos
#12
Piotr Chylenski, Dejan M Petrović, Gerdt Müller, Marie Dahlström, Oskar Bengtsson, Martin Lersch, Matti Siika-Aho, Svein Jarle Horn, Vincent G H Eijsink
BACKGROUND: Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass, especially the discovery of lytic polysaccharide monooxygenases (LPMOs), have opened new perspectives for process design and optimization. Softwood biomass is an abundant resource in many parts of the world, including Scandinavia, but efficient pretreatment and subsequent enzymatic hydrolysis of softwoods are challenging. Sulfite pulping-based pretreatments, such as in the BALI™ process, yield substrates that are relatively easy to degrade...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28696037/unravelling-biocomplexity-of-electroactive-biofilms-for-producing-hydrogen-from-biomass
#13
Alex J Lewis, Maria F Campa, Terry C Hazen, Abhijeet P Borole
Leveraging nature's biocomplexity for solving human problems requires better understanding of the syntrophic relationships in engineered microbiomes developed in bioreactor systems. Understanding the interactions between microbial players within the community will be key to enhancing conversion and production rates from biomass streams. Here we investigate a bioelectrochemical system employing an enriched microbial consortium for conversion of a switchgrass-derived bio-oil aqueous phase (BOAP) into hydrogen via microbial electrolysis (MEC)...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28691765/cooking-with-active-oxygen-and-solid-alkali-caosa-a-promising-alternative-approach-for-lignocellulosic-biorefineries
#14
Yetao Jiang, Xianhai Zeng, Rafael Luque, Xing Tang, Yong Sun, Tingzhou Lei, Shijie Liu, Lu Lin
Lignocellulosic biomass, a matrix of the biopolymers including cellulose, hemicellulose and lignin, has gathered increasing attention in recent years in the production of chemicals, fuels and materials based on biorefinery processes due to its renewability and availability. Fractionating lignocellulose is considered as the foundational step to establish an economical and sustainable lignocellulosic biorefinery. This Minireview summarized a newly developed oxygen delignification for lignocellulose fractionation called cooking with active oxygen and solid alkali (CAOSA), which could fractionate lignocellulose into its constituents while remaining in processable form...
July 10, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28690167/evaluation-of-lignins-from-side-streams-generated-in-an-olive-tree-pruning-based-biorefinery-bioethanol-production-and-alkaline-pulping
#15
José I Santos, Úrsula Fillat, Raquel Martín-Sampedro, María E Eugenio, María J Negro, Ignacio Ballesteros, Alejandro Rodríguez, David Ibarra
In modern lignocellulosic-based biorefineries, carbohydrates can be transformed into biofuels and pulp and paper, whereas lignin is burned to obtain energy. However, a part of lignin could be converted into value-added products including bio-based aromatic chemicals, as well as building blocks for materials. Then, a good knowledge of lignin is necessary to define its valorisation procedure. This study characterized different lignins from side-streams produced from olive tree pruning bioethanol production (lignins collected from steam explosion pretreatment with water or phosphoric acid as catalysts, followed by simultaneous saccharification and fermentation process) and alkaline pulping (lignins recovered from kraft and soda-AQ black liquors)...
July 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28688047/assessment-of-antioxidant-and-antimicrobial-properties-of-lignin-from-corn-stover-residue-pretreated-with-low-moisture-anhydrous-ammonia-and-enzymatic-hydrolysis-process
#16
Mingming Guo, Tony Jin, Nhuan P Nghiem, Xuetong Fan, Phoebe X Qi, Chan Ho Jang, Lingxiao Shao, Changqing Wu
Lignin accounts for 15-35% of dry biomass materials. Therefore, developing value-added co-products from lignin residues is increasingly important to improve the economic viability of biofuel production from biomass resources. The main objective of this work was to study the lignin extracts from corn stover residue obtained from a new and improved process for bioethanol production. Extraction conditions that favored high lignin yield were optimized, and antioxidant and antimicrobial activities of the resulting lignin were investigated...
July 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28683391/biodegradable-alternative-for-removing-toxic-compounds-from-sugarcane-bagasse-hemicellulosic-hydrolysates-for-valorization-in-biorefineries
#17
T Silva-Fernandes, J C Santos, F Hasmann, R C L B Rodrigues, H J Izario Filho, M G A Felipe
Among the major challenges for hemicellulosic hydrolysate application in fermentative processes, there is the presence of toxic compounds generated during the pretreatment of the biomass, which can inhibit microbial growth. Therefore, the development of efficient, biodegradable and cost-effective detoxification methods for lignocellulosic hydrolysates is crucial. In this work, two tannin-based biopolymers (called A and B) were tested in the detoxification of sugarcane bagasse hydrolysate for subsequent fermentation by Candida guilliermondii...
June 15, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28676418/waste-derived-bioeconomy-in-india-a-perspective
#18
REVIEW
Venkata Mohan S, Chiranjeevi P, Shikha Dahiya, Naresh Kumar A
Environmental and climatic change issues, population explosion, rapid urbanization, depletion of fossil reserves, need for energy security, huge waste generation, etc. are some of the inherent issues associated with the fossil based linear economy which need sustainable attention. In this realm, the world is gradually transforming from fossil-based economy to a sustainable circular economy. The biogenic waste which is generated in enormous quantity in India can be considered as potential feedstock for structuring the bio-based economy...
July 1, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28669588/gh62-arabinofuranosidases-structure-function-and-applications
#19
REVIEW
Casper Wilkens, Susan Andersen, Claire Dumon, Jean-Guy Berrin, Birte Svensson
Motivated by industrial demands and ongoing scientific discoveries continuous efforts are made to identify and create improved biocatalysts dedicated to plant biomass conversion. α-1,2 and α-1,3 arabinofuranosyl specific α-l-arabinofuranosidases (EC 3.2.1.55) are debranching enzymes catalyzing hydrolytic release of α-l-arabinofuranosyl residues, which decorate xylan or arabinan backbones in lignocellulosic and pectin constituents of plant cell walls. The CAZy database classifies α-l-arabinofuranosidases in Glycoside Hydrolase (GH) families GH2, GH3, GH43, GH51, GH54 and GH62...
June 29, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28665433/fast-microwave-assisted-acidolysis-a-new-biorefinery-approach-for-the-zero-waste-utilisation-of-lignocellulosic-biomass-to-produce-high-quality-lignin-and-fermentable-saccharides
#20
Long Zhou, Fabio Santomauro, Jiajun Fan, Duncan Macquarrie, James Clark, Christopher J Chuck, Vitaliy Budarin
Generally, biorefineries convert lignocellulosic biomass into a range of biofuels and further value added chemicals. However, conventional biorefinery processes focus mainly on the cellulose and hemicellulose fractions and therefore produce only low quality lignin, which is commonly burnt to provide process heat. To make full use of the biomass, more attention needs to be focused on novel separation techniques, where high quality lignin can be isolated that is suitable for further valorisation into aromatic chemicals and fuel components...
June 30, 2017: Faraday Discussions
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