Erradicate

With the aim to erradicate Enzootic Bovine Leukosis from Poland, a more sensitive real-time polymerase chain reaction was required and developed to detect proviral Bovine leukaemia virus (BLV) DNA, the causative agent of Enzootic Bovine Leukosis (EBL). This new method proved more sensitive for our needs, than the current protocols available in the public domain. DNA was extracted from peripheral blood leukocytes of 51 cattle, which had given rise to doubtful serological test results by ELISA, and from mesenteric lymph nodes of six cattle that were slaughtered as EBL suspect cases...

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Just say NO to biofilms: NO-donors are used to disperse a bacterial biofilm so that co-administered antibiotics will kill the more susceptible unattached cells. The chemically stable cephalosporin-3'-diazeniumdiolate NO-donor prodrug is activated by bacterial β-lactamases and facilitates this two-step biofilm erradication.

Diabetes is a chronic disease and as a consequence of the overproduction of reactive oxygen species (ROS), is related with oxidative stress. There are different sources of ROS, of which mitochondria is the main one. Oxidative stress seems to play an important role in mitochondria- mediated disease processes, though the exact molecular mechanisms responsible remain elusive. There are evidences which supports the idea that impaired mitochondrial function is a cause of the insulin insensitivity in different type of cells that arised as a result of an insufficient supply of energy or defects in the insulin signaling pathway...

BACKGROUND: Ventricular assist devices (VAD) are implanted with a growing frequency in patients with end-stage heart failure. In spite of technical and therapeutic advances, there is still a high incidence of device infections and healing disturbances with a considerable mortality. Reconstructive plastic surgery is able to provide help in erradicating infections and covering defects. PATIENTS AND METHODS: 11 patients with device infections and soft tissue defects were treated in our institution...

BACKGROUND: Sequential therapy is used as an alternative to growing antibiotic resistance of Helicobacter pylori to the standard triple therapy. Despite the success it had in Europe, we have no information regarding this therapy in our region. OBJECTIVES: To evaluate the eradication rate of Helicobacter pylori using sequential therapy and show its adverse effects. METHODS: We performed a prospective, observational, open descriptive study. 31 patients were evaluated who were treated with sequential therapy in the following way: the first 5 days omeprazole 20 mg and amoxicillin 1 g every 12 hours and following 5 days omeprazole 20 mg, clarithromycin 500 mg and tinidazole 500 mg every 12 hours...

OBJECTIVE: Show the efficacy of a tumor prosthesis after the resection of a giant cell knee tumor in the proximal tibia and the recovery of knee function. MATERIAL AND METHODS: This is a report of a retrospective observational clinical case of a male, 36-year-old patient with history of left knee arthrodesis in 1996 following the resection of a giant cell tumor in the distal femur. The latter relapsed in 2004 and a solitary pulmonary tumor node was detected. Broad resection of the femur and the arthrodesed tibia was performed together with the placement of a tumor prosthesis...

OBJECTIVE: To determine whether erradication of Helicobacter pylori (HP) infection from gastric mucosae is associated with changes in symptoms severity and satisfaction degree in patients with non-ulcer dyspepsia (NUD). METHODS: Prospective cohort study in patients with NUD and HP infection that were evaluated for six months following treatment, made up of 70 HP Negative patients in whom HP infection was erradicated, and 28 HP Positive patients in whom infection was not eradicated following treatment...

Radiation therapy (radiotherapy) is the medical use of ionizing radiation as part of cancer treatment to erradicate malignant cells. Normal tissue tolerance is currently a major dose-limiting factor. As molecular oxygen plays a critical role in creating the radiation damage, we propose a novel approach, that is, the use of acoustic radiation force (ARF) to suppress the normal tissue oxygenation, for the purpose of protecting the normal tissue and increasing its tolerance during radiotherapy. This paper investigated the effects of ARF on tissue oxygenation...

A new rifamycin derivative 3-(4-cinnamyl-piperazinyl iminomethyl) rifamycin SV (T9) and its sodium salt (T11, Rifacinna((R))) were in vitro, in vivo, toxicologically and clinically investigated in comparison with rifampicin, rifapentine, rifabutin, rifalazil. Our experiments showed that Rifacinna exhibits excellent in vitro activity against Gram(+), Gram (-) aerobic, anaerobic pathogens and mycobacteria. Rifacinna is active against Staphylococcus, Streptococcus spp. including MRSA, with MIC90- 0.06-0.5 mg/L; against Gram(+), Gram (-) anaerobes with MIC90 0...

OBJECTIVE: To validate an epidemiological score for identifying dyspeptic patients at high risk of being H. pylori positive. METHODS: Cross-sectional study including 434 users of primary health care units in the city of Pelotas, Southern Brazil, aged 18-45 years, and with symptoms of non-investigated dyspepsia, between 2006 and 2007. Dyspepsia was diagnosed according to Roma-II. The gold standard for H. pylori infection was the (13)C-urea-breath-test. The association between presence of H...

The patient with bleeding esophageal varices represents always a challenge to the medical team carrying a high mortality in spite of advances in pharmacologic and endoscopic treatments, notwithstanding that bleeding is just one added complication in the setting of the ailments of portal hypertension. Sclerotherapy, once the treatment of choice, has been displaced in recent year by variceal ligature, with less morbidity and equal success rate, both of them being technically simple. In the same way, variceal ligature has displaced sclerotherapy as treatment for secondary prophylaxis, with less complications and less sessions needed for variceal erradication and bleeding prevention...

OBJECTIVE: to assess the efficacy of rabeprazole (RPZ), amoxicillin (Am), and clarithromycin (Cla) (7 vs. 14 days) in the eradication of H. pylori, and to determine the effect of strain-specific antibiotic resistance and host CYP2C19 status. MATERIAL AND METHODS: first, we determined the CYP2C19 status of 100 healthy subjects to establish a sample size for the clinical trial. Then, 59 H. pylori-infected patients were randomized to receive RPZ (20 mg daily) plus Cla (500 mg b...

The rate of deep wound infections in spinal lumbar fusions is around 0.7% to 11.6%, being one of the causes of morbidity in acute phase. The aim of this study was to evaluate the management of spinal infection after internal lumbar fusions. Two hundred and sixty patients, who underwent to spinal surgery with lumbar fusion and iliac bone grafting, were analized, from January 1997 to January 2005. Wound infection was observed in eight (3%) cases. The average of age was 56 years, with a higher prevalence in males (5 patients)...

Every year, forty percent of the world population is at risk of contracting malaria. Hopes for the erradication of this disease during the 20th century were dashed by the ability of Plasmodium falciparum, its most deadly causative agent, to develop resistance to available drugs. Efforts to produce an effective vaccine have so far been unsuccessful, enhancing the need to develop novel antimalarial drugs. In this review, we summarize our knowledge concerning existing antimalarials, mechanisms of drug-resistance development, the use of drug combination strategies and the quest for novel anti-plasmodial compounds...

BACKGROUND: Hyperbaric oxygen therapy (HBO) leads to a significant increase of oxygen supply in poorly perfused tissue. Several physiological mechanisms of this treatment modality are suitable for having a positive effect on infected large autologous free bone transplants. CASE REPORT: In a 49-year-old male with a metastasized squamous cell carcinoma of the floor of the mouth radical tumour surgery with subsequent radiation was performed. Nine years later an infected osteoradionecrosis of the mandible emerged which required a continuity-resection with simultaneously performed reconstruction of the mandible using an autologous free transplanted iliac bone graft...

The Venezuelan Creole breed cattle is a Bos taurus well adapted to tropical conditions; however, it has demonstrated a low fertility rate. Recently, improvement in animal production by selection based on chromosomal analysis has allowed for the erradication of abnormalities involved in fertility problems, especially that of the 1 29 translocation. In the present work chromosomal analyses were carried out on 60 Creole bulls. Heparinized peripheral blood cells were cultured rendering metaphase spreads and subsequently stained by G- and C-banding techniques...

The erradication of poliomyelitis in the world is a goal that requires the adoption of effective and safe strategies for its attainment. Knowing how long the strains of poliovirus derived from the oral attenuated virus vaccine may circulate and remain in the environment was essential to define the measures to be taken and was also the objective of our paper. Specimens of stools and sewage water, which were weekly obtained at the end of the National Polio Vaccination Campaign, in 1998, were analyzed. Viruses were isolated and identified by culture and neutralization tests for the identification of poliovirus...

PURPOSE OF THE STUDY: Authors aimed to analyse indications for third and more than three times cesarean sections (c. s.), as well as, to make an assessment of the adequacy of the taken decisions. METHODS AND MATERIALS: It was conducted a retrospective study for third and more than three times cesarean, including period 1991-2003 in the Department of Obstetrics and Gynaecology, Medical University, Pleven. During that time 28645 births has taken place, 2971 of which are by cesarean section...

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