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Jing Zhu, Xiao Li, Hao Qi, Zetong Gu, Shangxin Song, Xiangli Yang, Guanghong Zhou, Chunbao Li
We investigated the impact of six protein diets on oxidation and anti-oxidation status in the muscle of young rats. Rats were fed six protein diets for 14 days, including casein (control), and proteins isolated from soy, fish, chicken, pork and beef. Grx1, Trx1 and other oxidative metabolic indices in muscle were quantified. Compared with the casein diet, the soy protein diet had a similar oxidation level, but higher GSH and lower SOD activities. The chicken and fish protein groups had lower GSH and higher SOD activities, the pork protein group showed lower Grx1 levels than the casein group and the beef protein group showed the highest GSH, Grx1 and Trx1 levels as reflected by RT-PCR, Western blotting and immunohistochemistry analyses...
October 13, 2017: Scientific Reports
Gregory H Tyson, Epiphanie Nyirabahizi, Emily Crarey, Claudine Kabera, Claudia Lam, Crystal Rice-Trujillo, Patrick F McDermott, Heather Tate
Bacteria of the genus Enterococcus are important human pathogens that are frequently resistant to a number of clinically important antibiotics. They are also used as a marker of animal fecal contamination of human foods, and are employed as sentinel organisms for tracking trends in resistance to antimicrobials with gram-positive activity. As part of the National Antimicrobial Resistance Monitoring System (NARMS), we evaluated several retail meat commodities for the presence of enterococci from 2002-2014, finding 92...
October 13, 2017: Applied and Environmental Microbiology
Kamonnaree Chotinantakul, Nittaya Chansiw, Seiji Okada
OBJECTIVES: The aim of this study is to evaluate the prevalence of resistant phenotypes and genes of Enterococcus spp. to explore the range of resistant profiles from Thai traditional fermented pork. METHODS: A total of 120 Thai fermented pork specimens in Chiang Rai, Thailand were collected and antimicrobial resistance was identified using 11 antimicrobial agents in enterococcal isolates, via an agar disk diffusion method. The antibiotic resistant genes from resistant phenotypes and virulence genes were observed...
October 10, 2017: Journal of Global Antimicrobial Resistance
Ying Sun, Yiming Pang, Jingxu Zhang, Zhiwen Li, Jufen Liu, Bin Wang
High-accuracy analyses of polycyclic aromatic hydrocarbons (PAHs) in lipid matrix-based biological samples are highly necessary. We investigated the cleanup performance of the commercially available molecularly imprinted polymers (MIPs) to analyze PAHs in various biological samples (i.e., butter, peanut oil, pork belly, and human umbilical cord), and compared this method with the commonly used gel permeation chromatography (GPC). After primary cleanup with a MIP or GPC column, the extracts were further cleaned with a neutral aluminum oxide column...
October 12, 2017: Analytical and Bioanalytical Chemistry
Richard P Smith, Victor Andres, Francesca Martelli, Becky Gosling, Francisco Marco-Jimenez, Kelly Vaughan, Monika Tchorzewska, Rob Davies
AIMS: The control of Salmonella in pig production is necessary for public and animal health and vaccination was evaluated as a strategy to decrease pig prevalence. METHODS AND RESULTS: The study examined the efficacy of a live Salmonella Typhimurium vaccine, administered to sows on eight commercial farrow-to-finish herds experiencing clinical salmonellosis or Salmonella carriage associated with S. Typhimurium or its monophasic variants. Results of longitudinal Salmonella sampling were compared against eight similarly selected and studied control farms...
October 10, 2017: Journal of Applied Microbiology
Maicon Sbardella, Aline M C Racanicci, Franz D Gois, Cristiane B de Lima, Dannielle L Migotto, Leandro B Costa, Valdomiro S Miyada
BACKGROUND: The effects of dietary levels of hop β-acids on physical attributes, lipid oxidation, and chemical composition of pork meat was evaluated. Thirty-two castrated male pigs obtained from a complete block design feeding experiment (6.23 ± 0.42 kg initial BW to 20.45±0.95 kg final BW) and fed diets supplemented with 0, 120, 240 or 360 mg kg(-1) hop β-acids during 35-d were slaughtered to sample Longissimus dorsi muscle for meat analysis. RESULTS: No effects (P>0...
October 11, 2017: Journal of the Science of Food and Agriculture
Aiguo Luo, Jia Feng, Bianfang Hu, Junping Lv, C-Y Oliver Chen, Shulian Xie
This study examined the effect of Spirulina platensis polysaccharides (SPP) at 0.1%, 0.25%, and 0.5% (wt/wt) of Chinese-style sausages on lipid peroxidation, microbiological and sensory properties during 24 d stored at 4 °C. During the storage, pH, lightness (L(*) ) values, DPPH radical scavenging activity and sensory scores decreased with time and TBARS, TVB-N, mesophilic, and psychrotrophic total viable counts increased. However, the magnitude of the changes was attenuated with the addition of SPP as compared to control...
October 12, 2017: Journal of Food Science
Nuntiput Putthanachote, Supannee Promthet, Cameron Hurst, Krittika Suwanrungruang, Peechanika Chopjitt, Surapon Wiangnon, Sam Li-Sheng Chen, Amy Ming-Fang Yen, Tony Hsiu-Hsi Chen
BACKGROUND: Previous studies have found that polymorphisms of the DNA repair gene X-ray repair cross-complementing group 1(XRCC1) and environmental factors are both associated with an increased risk of stomach cancer, but no study has reported on the potential additive effect of these factors among Thai people. The aim of this study was to investigate whether the risk of stomach cancer from XRCC1 gene polymorphisms was modified by environmental factors in the Thai population. METHODS: Hospital-based matched case-control study data were collected from 101 new stomach cancer cases and 202 controls, which were recruited from2002 to 2006 and were matched for gender and age...
October 11, 2017: BMC Cancer
Simone Belluco, Giulia Simonato, Marzia Mancin, Mario Pietrobelli, Antonia Ricci
BACKGROUND: Toxoplasmosis is a zoonotic disease causing severe symptoms in pregnant women and immunocompromised individuals. On average, worldwide, around 30% of people are seropositive. The oral transmission route is of great significance and food, particularly meat, is an important transmission vehicle for T. gondii. However, the role of different food matrices is debated. OBJECTIVES: The aim of this review was to assess the risk of humans developing acute T. gondii infection via the foodborne route...
October 11, 2017: Critical Reviews in Food Science and Nutrition
Ye Zou, Huan Bian, Pengpeng Li, Zhilan Sun, Chong Sun, Muhan Zhang, Zhiming Geng, Weimin Xu, Daoying Wang
The aim of this study was to investigate the optimal conditions of ultrasound-assisted alkaline extraction (UAAE) on pork liver protein isolate (UPLPI) and its physico-chemical properties. Response surface methodology was used to determine the optimal conditions for UAAE, which were at ultrasonic power 265 W, ultrasonic time 42 min, NaOH concentration 0.80%, temperature 50°C, and solvent/raw material ratio 70. The extraction yield and efficiency of UPLPI were significantly improved over the conventional alkaline extraction (PLPI)...
October 10, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
Alberto Arnedo-Pena, Iraya Vivas-Fornas, Noemi Meseguer-Ferrer, María Dolores Tirado-Balaguer, Alberto Yagüe-Muñoz, Silvia Herrera-León, Susana Sabater-Vidal, María Ángeles Romeu-García, Ana Vizcaino Batllés, Juan Bautista Bellido-Blasco, Rosario Moreno-Muñoz
INTRODUCTION: Salmonella infections (SI) are common in Spain. The aim of this study was to appraise risk factors and the clinical characteristics of sporadic Salmonella Typhimurium infections compared with other sporadic salmonella serotype infections (OSI). METHODS: From September 2014 to August 2015, a case-case study was carried out by the Epidemiology Division of the Public Health Centre of Castellon. Case 1 consisted of patients with sporadic S. Typhimurium infections, while case 2 comprised OSI patients, assessed according to the stool cultures analyzed by the Microbiology Laboratories of Hospital General de Castellon and Hospital de La Plana in Vila-real...
October 7, 2017: Enfermedades Infecciosas y Microbiología Clínica
J M Martins, A Albuquerque, J A Neves, A B Freitas, R Charneca, J L Tirapicos
Data evaluating the effect of oleic-acid-enriched diets and outdoor rearing (with access to pasture) on the quality of Alentejano (AL) pork and fat are limited. The aim of this work was to study the effects of feeding low (LO) or high oleic acid (HO) diets to AL pigs reared in individual pens (IND) or outdoors (OUT) with access to pasture. Biceps femoris and dorsal subcutaneous fat (DSF) samples were collected to analyse chemical composition and lipids fatty acid (FA) profile. The expression of seven genes involved in lipogenesis, lipolysis and FA transport, as well as two transcription factors were also evaluated in the muscle...
October 8, 2017: Journal of Animal Physiology and Animal Nutrition
Jiaquan Xu, Shengrui Xu, Yipo Xiao, Konstantin Chingin, Haiyan Lu, Runhan Yan, Huanwen Chen
Rapid quantitative determination of bulk molecular concentration in solid samples without sample pretreatment is demonstrated by the internal extractive electrospray ionization mass spectrometry (iEESI-MS) analysis of six β-agonists, including salbutamol (Sal), clenbuterol (Cle), ractopamine (Rac), terbutaline (Ter), tulobuterol (Tul), brombuterol (Bro), in pork tissue samples. Single sample analysis only required 1 min. The linear range of detection was about 0.01-1000 μg/kg (R2>0.9994). The limit-of-detection (LOD) varied from 0...
October 9, 2017: Analytical Chemistry
Wei-Liang Wu, Xiao-Ling Deng, Shao-Jun Zhou, Hui Liang, Xing-Fen Yang, Jian Wen, Xiao-Ming Li, Chao-Zheng Zhang, Yong-Hui Zhang, Fei Zou
Persistent organic pollutants such as polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like polychlorinated biphenyls (DL-PCBs) consisting of non-ortho and mono-ortho PCBs are suggested to be very hazardous and have adverse effects on human health. However, their levels and congener profiles in retail foods marketed in Guangdong Province of China have not been elucidated thus far. Thus, in this study, 226 individual samples of beef, freshwater fish, and pork marketed across four regions of Guangdong Province were randomly collected during 2013-2015 to determine their levels of PCDD/Fs and DL-PCBs...
October 4, 2017: Science of the Total Environment
C Siamupa, N Saasa, A M Phiri
African swine fever (ASF) is a worldwide disease of pigs endemic in most sub-Saharan African countries. Zambia has been experiencing outbreaks of ASF for many years because the disease is endemic in the eastern part of the country, with incursion into the central part of Lusaka Province. The latest outbreaks of ASF in Lusaka occurred in 2013 with substantial pig mortalities, loss in trade, and cost of control measures and compensation of affected farmers. The aims of the study were to identify market value chain-related factors that were associated with ASF outbreaks and assess why these outbreaks are becoming frequent despite control measures being put in place...
October 6, 2017: Tropical Animal Health and Production
Fen-Fen Chen, Yan Xiong, Ying Peng, Yun Gao, Jin Qin, Gui-Yan Chu, Wei-Jun Pang, Gong-She Yang
Intramuscular fat (IMF) content affects the tenderness, juiciness, and flavor of pork. An increasing number of studies are focusing on the functions of microRNAs (miRs) during porcine intramuscular preadipocyte development. Previous studies have proved that miR-425-5p was enriched in porcine skeletal muscles and played important roles in multiple physiological processes; however, its functions during intramuscular adipogenesis remain unclear. To explore the role of miR-425-5p in porcine intramuscular adipogenesis, miR-425-5p agomir and inhibitor were used to perform miR-425-5p overexpression and knockdown in intramuscular preadipocytes, respectively...
October 6, 2017: International Journal of Molecular Sciences
S Bonardi, I Bruini, L Bolzoni, P Cozzolino, M Pierantoni, F Brindani, P Bellotti, M Renzi, S Pongolini
The inactivation of Salmonella during curing of Italian traditional pork salami was investigated. A total of 150 batches of ground raw meat (GRM) used for salami manufacturing by four producers were tested for Salmonella by real-time PCR followed by ISO 6579 cultural confirmation and MPN enumeration. Salami produced with Salmonella positive GRMs were re-tested at the end of their curing period. Aw, pH and NaCl content were also measured. Detection of Salmonella was performed testing both 25 and 50g of the samples...
December 4, 2017: International Journal of Food Microbiology
Macarena Egea, Mª Belén Linares, Marta Gil, Mª Belén López, Mª Dolores Garrido
BACKGROUND: Consumers highly sensitive to androstenone will probably reject meat from entire male pigs, which tends to have high levels of this hormone. To avoid this, the effect of different masking strategies (sprinkling with mixed spices or fennel, marinating and breading with garlic-parsley or curry) on the sensory parameters of pork loin chops obtained from entire animals with high levels of androstenone (AND) (1.0-2.9 mg kg (-1) AND in fat) and castrated animals (<0.4 mg kg (-1) AND in fat), both with low levels of skatole (SKA) (<0...
October 5, 2017: Journal of the Science of Food and Agriculture
N A Sidorova, V L Popov, O S Lavrukova, A N Prikhod'ko, S N Lyabzina, E I Tikhomirova
The objective of the present study was to characterize the specific features of corpse putrification under the influence of necrobiome enzymatic systems depending on the duration of the post-mortem period. We present the results of investigations into the enzymatic activity of the dominant species of microorganisms making up the post-mortem microbiome. The domestic pork carcasses weighing 50-70 kg were used as an experimental putrification model. The study revealed the characteristic features of protein decomposition under the influence of proteolytic enzymes of pseudomonads, bacilli, and clostridia, such as alteration in the amount of necrobionts producing proteases in the entire carcass and its fragments during biodegradation in the air over 30 and 136 days of the post-mortem period...
2017: Sudebno-meditsinskaia Ekspertiza
Victor Neira, Natalia Ramos, Rodrigo Tapia, Juan Arbiza, Andrónico Neira-Carrillo, Manuel Quezada, Álvaro Ruiz, Sergio A Bucarey
BACKGROUND: Porcine circovirus type 2 (PCV2) is a very small, non-enveloped and icosahedral virus, with circular single stranded DNA genome. This virus is the most ubiquitous and persistent pathogen currently affecting the swine industry worldwide. PCV2 has been implicated as the major causative agent of postweaning multisystemic wasting syndrome (PMWS), a disease which is characterized by severe immunosuppressive effects in the porcine host. Worldwide PCV2 isolates have been classified into four different genotypes, PCV2a, PCV2b, PCV2c and PCVd...
October 4, 2017: Virology Journal
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