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https://www.readbyqxmd.com/read/28438938/a-tick-antivirulence-protein-potentiates-antibiotics-against-staphylococcus-aureus
#1
Nabil M Abraham, Lei Liu, Brandon L Jutras, Kristen Murfin, Ali Acar, Timur O Yarovinsky, Erica Sutton, Martin Heisig, Christine Jacobs-Wagner, Erol Fikrig
New strategies are needed to combat antibiotic resistance, especially against pathogens such as methicillin-resistant Staphylococcus aureus A tick antifreeze glycoprotein, IAFGP, possess potent anti-biofilm properties against a variety of clinical pathogens including S. aureus Synergy between IAFGP, or a peptide (P1) representative of a repeat region of the protein, with different antibiotics was assessed in vitro Antibiotics that synergized with either IAFPG or P1 were further evaluated in vivo using vertebrate and invertebrate infection models...
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28438061/the-pattern-and-magnitude-of-invivo-thrombin-generation-differ-in-women-with-preeclampsia-and-in-those-with-sga-fetuses-without-preeclampsia
#2
Offer Erez, Roberto Romero, Edi Vaisbuch, Juan Pedro Kusanovic, Shali Mazaki-Tovi, Tinnakorn Chaiworapongsa, Francesca Gotsch, Pooja Mittal, Samuel S Edwin, Chia-Ling Nhan-Chang, Nandor Gabor Than, Chong Jai Kim, Sun Kwon Kim, Lami Yeo, Moshe Mazor, Sonia S Hassan
OBJECTIVE: We aimed to determine the differences in the pattern and magnitude of thrombin generation between patients with preeclampsia (PE) and those with SGA. METHODS: This cross-sectional study included women in the following groups: 1) normal pregnancy (NP) (n = 49); 2) PE (n = 56); and 3) SGA (n = 28). Maternal plasma thrombin generation (TGA) was measured, calculating: a) lag time (LT); b) velocity index (VI); c) peak thrombin concentration (PTC); d) time-to-peak thrombin concentration (TPTC); and e) endogenous thrombin potential (ETP)...
April 24, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28411451/anisotropic-tough-double-network-hydrogel-from-fish-collagen-and-its-spontaneous-in%C3%A2-vivo-bonding-to-bone
#3
Md Tariful Islam Mredha, Nobuto Kitamura, Takayuki Nonoyama, Susumu Wada, Keiko Goto, Xi Zhang, Tasuku Nakajima, Takayuki Kurokawa, Yasuaki Takagi, Kazunori Yasuda, Jian Ping Gong
Soft supporting tissues in the human body, such as cartilages and ligaments, are tough materials and firmly fixed to bones. These soft tissues, once injured, cannot regenerate spontaneously in vivo. Developing tough and biocompatible hydrogels as artificial soft supporting tissues would substantially improve outcomes after soft tissue injury. Collagen is the main rigid component in soft connective tissues which is organized in various hierarchical arrays. We have successfully developed a novel class of collagen fibril-based tough hydrogels based on the double network (DN) concept using swim bladder collagen (SBC) extracted from Bester sturgeon fish...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28405827/mgmt-promoter-methylation-status-in-merkel-cell-carcinoma-in-vitro-versus-invivo
#4
Giuseppina Improta, Cathrin Ritter, Angela Pettinato, Valeria Vasta, David Schrama, Filippo Fraggetta, Jürgen C Becker
PURPOSE: Expression of O6-methylguanine-DNA methyltransferase (MGMT) in Merkel cell carcinoma (MCC) is very variable; thus, we tested whether this may be due to differential methylation of the MGMT gene promoter. METHODS: Quantitative analysis of MGMT mRNA and protein expression, as well as MGMT promoter methylation status, was performed in a series of tissue samples of MCC tumors, representing both primary and metastatic lesions, as well as in six MCC cell lines...
April 12, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28394817/comparative-bone-regeneration-potential-studies-of-collagen-heparin-and-polydopamine-coated-multichannelled-bcp-granules
#5
Gun Hee Lee, Preeti Makkar, Kallyanshis Paul, Byong Taek Lee
The present work is a comparative assessment of the bone regeneration potentiality of bone substitutes comprised of bioactive polymer coated biphasic calcium phosphate (BCP) granules. The bone substitutes were primarily composed of multichanneled BCP granules which were coated separately with biochemical polymer coatings, namely collagen, heparin or polydopamine, using chemical methods. The morphological features and chemical structure of the granules and surface coatings were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) respectively...
April 6, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28390086/virtual-screening-and-biological-evaluation-of-novel-antipyretics-compounds
#6
Thamires Quadros Froes, Miriam C C Melo, Gloria E P Souza, Marcelo Santos Castilho, Denis M Soares
Due to the absence ofsafety of the antipyretics to patients withcardiovascular dysfunction,new targets to treatinflammation have been pursued. mPGES-1 is a promising target because its inhibition would not cause the side effectsrelated to COX inhibition. In order to identify novel inhibitorsof mPGES-1, we developed a ligand-basedpharmacophoremodel that differentiates true inhibitors from decoys andenlightens the structure-activity relationships for known mPGES-1 inhibitors.The model (4 hydrophobic centers, 2 hydrogen-bond acceptor and 2 hydrogen-bond donor points) was employed to select lead-like compoundsfrom ZINC database forin vivo evaluation...
April 8, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28371660/exposure-of-heligmosomoides-polygyrus-and-trichuris-muris-to-albendazole-albendazole-sulfoxide-mebendazole-and-oxantel-pamoate-in%C3%A2-vitro-and-in%C3%A2-vivo-to-elucidate-the-pathway-of-drug-entry-into-these-gastrointestinal-nematodes
#7
Noemi Cowan, Charles Meier, Anna Neodo, Jennifer Keiser
Millions of people are treated with anthelmintics to control soil-transmitted helminth infections; yet, drug distribution in the plasma and gastrointestinal tract compartments and the pathway of drug uptake into gastrointestinal nematodes responsible for the pharmacological effect are unknown. We assessed the distribution and uptake of albendazole, albendazole sulfoxide, albendazole sulfone in the hookworm Heligmosomoides polygyrus in vitro and in vivo as well as the distribution and uptake of albendazole, mebendazole, and oxantel pamoate in the whipworm Trichuris muris in vitro and invivo...
March 24, 2017: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/28362770/pupil-segmentation-adaptive-optics-for-invivo-mouse-retinal-fluorescence-imaging
#8
Daniel J Wahl, Christine Huang, Stefano Bonora, Yifan Jian, Marinko V Sarunic
Adaptive Optics (AO) for scanning laser ophthalmoscopy enables high-resolution retinal imaging that can be used for preclinical research of diseases causing vision loss. Pupil Segmentation (PS) is an approach to wavefront-sensorless AO that acquires images within subregions across the imaging pupil to measure the wavefront slopes at the corresponding locations of the beam. We present PS-AO as an approach to correct ocular aberrations in ∼7  s, implemented to minimize respiratory motion from an anesthetized mouse...
April 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28267666/rf-inhomogeneity-compensation-using-method-of-fourier-synthesis
#9
Navin Khaneja
In this paper, we propose a new method for design of composite pulses that are robust to rf-amplitude (rf-inhomogeneity). We call this, the method of Fourier synthesis. The method is general enough to design excitation, inversion, refocusing or arbitary flip angle pulses that are robust to rf-amplitude. The method can be tailored to have amplitude selective excitation. We experimentally show rf-compensation over a order of magnitude (20db) variation in rf-amplitude. The method is expected to find use in invivo NMR studies using surface coils, where there is large dispersion in rf-amplitude over the sample...
February 15, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28223335/cooperation-of-musashi-2-numb-mdm2-and-p53-in-drug-resistance-and-malignant-biology-of-pancreatic-cancer
#10
Weiwei Sheng, Ming Dong, Chuanping Chen, Zixin Wang, Yunwei Li, Kewei Wang, Yuji Li, Jianping Zhou
Our earlier work showed that Musashi (MSI)-2 promoted the development of pancreatic cancer (PC) by down-regulating Numb, which prevented murine double-minute (MDM)-2-mediated p53 ubiquitin degradation. Thus, we investigate the relationship among MSI2, Numb, MDM2, and p53 in PC in vitro and invivo, an association that has not been reported to our knowledge. MSI2 had no relationship with mutant p53 (mtp53) and wild-type p53 (wtp53) in normal PC cells. However, in response to gemcitabine or cisplatin treatment, MSI2 silencing simultaneously down-regulated MDM2 and up-regulated Numb and wtp53 protein levels...
February 21, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28193696/astrocytic-orosomucoid-2-modulates-microglial-activation-and-neuroinflammation
#11
Myungjin Jo, Jong-Heon Kim, Gyunjee Song, Minchul Seo, Eun Mi Hwang, Kyoungho Suk
Orosomucoid (ORM) is an acute-phase protein that belongs to the immunocalin subfamily, a group of small-molecule binding proteins with immunomodulatory functions. Little is known about the role of ORM proteins in the central nervous system. The aim of the present study was to investigate the brain expression of ORM and its role in neuroinflammation. Expression of Orm2, but not Orm1 or Orm3, was highly induced in the mouse brain after systemic injection of lipopolysaccharide (LPS). Plasma levels of ORM2 were also significantly higher in patients with cognitive impairment than in normal subjects...
February 13, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28179526/a-sequence-independent-unstructured-internal-ribosome-entry-site-is-responsible-for-internal-expression-of-the-coat-protein-of-turnip-crinkle-virus
#12
Jared May, Philip Johnson, Huma Saleem, Anne E Simon
To maximize the coding potential of viral genomes, internal ribosome entry sites (IRES) can be used to bypass the traditional requirement of a 5' cap and some/all of the associated translation initiation factors. Although viral IRES typically contain higher-order RNA structure, an unstructured sequence of about 84 nucleotides (nt) immediately upstream of the Turnip crinkle virus (TCV) coat protein (CP) open reading frame (ORF) has been found to promote internal expression of the CP from the genomic RNA (gRNA) both in vitro and in vivo An absence of extensive RNA structure was predicted using RNA folding algorithms and confirmed by selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) RNA structure probing...
April 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28089609/de-immunized-and-functional-therapeutic-deft-versions-of-a-long-lasting-recombinant-alpha-interferon-for-antiviral-therapy
#13
Eduardo F Mufarrege, Sofía Giorgetti, Marina Etcheverrigaray, Frances Terry, William Martin, Anne S De Groot
Interferon α (IFN-α) exerts potent antiviral, immunomodulatory, and antiproliferative activity and have proven clinical utility in chronic hepatitis B and C virus infections. However, repeated IFN-α administration induces neutralizing antibodies (NAb) against the therapeutic in a significant number of patients. Associations between IFN-α immunogenicity and loss of efficacy have been described. So as to improve the in vivo biological efficacy of IFN-α, a long lasting hyperglycosylated protein (4N-IFN) derived from IFN-α2b wild type (WT-IFN) was developed...
March 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28076290/tailored-interactive-sequences-for-continuous-mr-image-guided-freehand-biopsies-of-different-organs-in-an-open-system-at-1-0-tesla-t-initial-experience
#14
Florian Streitparth, Christian Althoff, Martin Jonczyk, Felix Guettler, Martin Maurer, Hendrik Rathke, Keno Moritz Sponheuer, Bernd Hamm, Ulf K Teichgräber, Maximilian de Bucourt
OBJECTIVES: To assess the feasibility, image quality, and accuracy of freehand biopsies of liver, bone, muscle, vertebral disc, soft tissue, and other lesions using balanced steady-state free precession (SSFP, balanced fast field echo: bFFE), spoiled and nonspoiled gradient echo (FFE), and turbo spin echo (TSE) sequences for interactive continuous navigation in an open magnetic resonance imaging (MRI) system at 1.0 tesla (T). METHODS: Twenty-six MR-guided biopsies (five liver, five bone, four muscle, four vertebral disc, one lung, one kidney, one suprarenal gland, and five soft or other tissue) were performed in 23 patients in a 1...
January 11, 2017: Biomedizinische Technik. Biomedical Engineering
https://www.readbyqxmd.com/read/28048356/su-g-tep4-13-interfraction-treatment-monitoring-using-integrated-invivo-epid-images
#15
D Defoor, N Papanikolaou, S Stathakis
PURPOSE: To investigate inter-fraction differences of dose delivery by analyzing portal images acquired during treatment and implement an automated system to generate a report for each fraction. Large differences in images between fractions can alert the physicist of possible machine performance issues or patient set-up errors. METHODS: A Varian Novalis Tx equipped with a HD120 MLC and aS1000 electronic portal imaging device (EPID) was used in our study. EPID images are acquired in continuous acquisition mode for 32 volumetric arc therapy (VMAT) patients...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/27972911/fabrication-of-anti-vitiligo-ointment-containing-psoralia-corylifolia-invitro-and-invivo-characterization
#16
I Hussain, N Hussain, A Manan, B Ali, A Rashid
No abstract text is available yet for this article.
November 2016: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27800485/clinical-application-of-cone-beam-computed-tomography-of-the-rabbit-head-part-1-normal-dentition
#17
G G Riggs, Boaz Arzi, Derek D Cissell, David C Hatcher, Philip H Kass, Amy Zhen, Frank J M Verstraete
Domestic rabbits (Oryctolagus cuniculus) are increasingly popular as household pets; therefore, veterinarians need to be familiar with the most common diseases afflicting rabbits including dental diseases. Diagnostic approaches for dental disease include gross oral examination, endoscopic oral examination, skull radiography, and computed tomography (CT). CT overcomes many limitations of standard radiography by permitting cross-sectional images of the rabbit head in multiple planes without superimposition of anatomic structures...
2016: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/27725960/drug-delivery-via-cell-membrane-fusion-using-lipopeptide-modified-liposomes
#18
Jian Yang, Azadeh Bahreman, Geert Daudey, Jeroen Bussmann, René C L Olsthoorn, Alexander Kros
Efficient delivery of drugs to living cells is still a major challenge. Currently, most methods rely on the endocytotic pathway resulting in low delivery efficiency due to limited endosomal escape and/or degradation in lysosomes. Here, we report a new method for direct drug delivery into the cytosol of live cells in vitro and invivo utilizing targeted membrane fusion between liposomes and live cells. A pair of complementary coiled-coil lipopeptides was embedded in the lipid bilayer of liposomes and cell membranes respectively, resulting in targeted membrane fusion with concomitant release of liposome encapsulated cargo including fluorescent dyes and the cytotoxic drug doxorubicin...
September 28, 2016: ACS Central Science
https://www.readbyqxmd.com/read/27720400/evaluation-of-quail-and-turkey-egg-yolk-for-cryopreservation-of-nili-ravi-buffalo-bull-semen
#19
S Akhter, B A Rakha, M S Ansari, A U Husna, S Iqbal, M Khalid
Egg yolk is used as a cryoprotectant for semen in different mammalian species including buffalo. Egg yolk from different sources may affect freezability of buffalo bull semen. Quail egg yolk (QEY) and turkey egg yolk (TEY) in Tris-citric acid extender was evaluated for post-thaw quality and in vivo fertility rate of cryopreserved buffalo bull semen. Ejaculates were collected on weekly basis from six Nili-Ravi buffalo bulls (12 ejaculates/bull) for a period of 6 weeks and diluted at 37 °C with tris-citric egg yolk extender (50 × 10(6) motile spermatozoa mL(-1)) containing different levels of QEY or TEY (5%, 10%, 15%, and 20%) or 20% chicken egg yolk (CEY; controls) and cryopreserved...
January 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/27692837/central-zone-lesions-on-magnetic-resonance-imaging-should-we-be-concerned
#20
Wei Phin Tan, Andrew Mazzone, Stephanie Shors, Nency Antoine, Shahid Ekbal, Narendra Khare, Charles McKiel, Dennis Pessis, Leslie Deane
INTRODUCTION AND OBJECTIVE: The Prostate Imaging Reporting and Data System (PI-RADS) score was developed to evaluate lesions in the peripheral and transition zone on multiparametric magnetic resonance imaging (mpMRI) of the prostate. We aim to determine if the PI-RADS scoring system can be used to evaluate central zone lesions on mpMRI. MATERIALS AND METHODS: A retrospective review of 73 patients who underwent mpMRI/ultrasound (US) fusion-guided biopsy of 143 suspicious lesions between February 2014 and October 2015 was performed...
January 2017: Urologic Oncology
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