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Jian Yang, Azadeh Bahreman, Geert Daudey, Jeroen Bussmann, René C L Olsthoorn, Alexander Kros
Efficient delivery of drugs to living cells is still a major challenge. Currently, most methods rely on the endocytotic pathway resulting in low delivery efficiency due to limited endosomal escape and/or degradation in lysosomes. Here, we report a new method for direct drug delivery into the cytosol of live cells in vitro and invivo utilizing targeted membrane fusion between liposomes and live cells. A pair of complementary coiled-coil lipopeptides was embedded in the lipid bilayer of liposomes and cell membranes respectively, resulting in targeted membrane fusion with concomitant release of liposome encapsulated cargo including fluorescent dyes and the cytotoxic drug doxorubicin...
September 28, 2016: ACS Central Science
S Akhter, B A Rakha, M S Ansari, A U Husna, S Iqbal, M Khalid
Egg yolk is used as a cryoprotectant for semen in different mammalian species including buffalo. Egg yolk from different sources may affect freezability of buffalo bull semen. Quail egg yolk (QEY) and turkey egg yolk (TEY) in Tris-citric acid extender was evaluated for post-thaw quality and in vivo fertility rate of cryopreserved buffalo bull semen. Ejaculates were collected on weekly basis from six Nili-Ravi buffalo bulls (12 ejaculates/bull) for a period of 6 weeks and diluted at 37 °C with tris-citric egg yolk extender (50 × 10(6) motile spermatozoa mL(-1)) containing different levels of QEY or TEY (5%, 10%, 15%, and 20%) or 20% chicken egg yolk (CEY; controls) and cryopreserved...
September 9, 2016: Theriogenology
Wei Phin Tan, Andrew Mazzone, Stephanie Shors, Nency Antoine, Shahid Ekbal, Narendra Khare, Charles McKiel, Dennis Pessis, Leslie Deane
INTRODUCTION AND OBJECTIVE: The Prostate Imaging Reporting and Data System (PI-RADS) score was developed to evaluate lesions in the peripheral and transition zone on multiparametric magnetic resonance imaging (mpMRI) of the prostate. We aim to determine if the PI-RADS scoring system can be used to evaluate central zone lesions on mpMRI. MATERIALS AND METHODS: A retrospective review of 73 patients who underwent mpMRI/ultrasound (US) fusion-guided biopsy of 143 suspicious lesions between February 2014 and October 2015 was performed...
September 27, 2016: Urologic Oncology
Stephen Michael Vaughan, Chung How Kau, Peter Daniel Waite
OBJECTIVES: To set forth a universal standard methodology for quantifying volumetric and linear changes in the craniofacial complex, utilizing three-dimensional data captured from a cleft-lip palate patient who underwent rigid external device (RED) distraction. METHODS: Cone beam computed tomography images of a 14-year-old patient were captured using a Kodak 9500 (Atlanta, GA) Cone Beam system device and a stereophotogrammetric system (3dMDface(TM) Atlanta, GA)...
September 2016: Journal of Craniofacial Surgery
Wei Phin Tan, Carol Lin, Meri Chen, Leslie A Deane
OBJECTIVE: To evaluate whether periprostatic fat volume and periprostatic fat ratio as determined by multiparametric magnetic resonance imaging (mpMRI) correlate with the presence of high-grade prostate cancer. MATERIALS AND METHODS: A total of 295 consecutive patients (median age: 64, range: 38-84) underwent mpMRI of the prostate gland between August 2013 and February 2015. All patients underwent a 3 Tesla mpMRI. Using DynaCAD (Invivo, Gainesville, FL), we calculated the prostate volume and volume of the periprostatic fat seen on mpMRI...
September 1, 2016: Urology
Levent Vahdettin, Seçil Aksoy, Ulaş Öz, Kaan Orhan
BACKGROUND/AIM: The aim of this study was to create a database of 3D cephalometric measurements of Turkish Cypriot patients using a 3D rendering software program. MATERIALS AND METHODS: The study population comprised 121 subjects who had undergone cone beam CT imaging (CBCT). Invivo 5.1 software was used to generate cephalograms from the CBCT dataset that were then linked to the 3D hard-tissue surface representations. In total, 38 angular and 28 linear widely used measurements were recorded...
2016: Turkish Journal of Medical Sciences
Ajay K Chaudhary, Shruti Chaudhary, Kanjaksha Ghosh, A Nadkarni
Controlled remodeling of the extracellular matrix (ECM) is essential for cell growth, invasion and metastasis. Matrix metalloproteinases (MMPs) are a family of secreted, zincdependent endopeptidases capable of degradation of ECM components. The expression and activity of MMPs in a variety of human cancers have been intensively studied. They play important roles at different steps of malignant tumor formation and have central significance in embryogenesis, tissue remodeling, inflammation, angiogenesis and metastasis...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Bhavana Sujanamulk, Rajalakshmi Chintamaneni, Anuradha Chennupati, Prashant Nahar, Rattaiah Setty Chaluvadi, Ramakrishna Vemugunta, Meka Venkata Poorna Prabhat
INTRODUCTION: Lawsonia Inermis (LI) is a shrub cultivated throughout India. Many in vitro studies have been done on antifungal activity of LI, although none of the studies have been conducted invivo. AIM: To evaluate the antifungal efficacy of ethanolic extract of crude lawsone in comparison with listerine mouth wash in known diabetics and wearing dentures. MATERIALS AND METHODS: A total of 60 subjects were taken and randomly divided into two groups of 30 each...
June 2016: Journal of Clinical and Diagnostic Research: JCDR
Hemant K Patel, Virendra Gajbhiye, Prashant Kesharwani, Narendra K Jain
The present investigation was aimed at developing various ligands-anchored dendrimers and comparing their brain targeting potential at one platform. Sialic acid (S), glucosamine (G) and concanavalin A (C) anchored poly(propyleneimine) (PPI) dendritic nanoconjugates were developed and evaluated for delivery of anti-cancer drug, paclitaxel (PTX) to the brain. MTT assay on U373MG human astrocytoma cells indicated IC50 values of 0.40, 0.65, 0.95, 2.00 and 3.50μM for PTX loaded SPPI, GPPI, CPPI, PPI formulations, and free PTX, respectively...
November 15, 2016: Journal of Colloid and Interface Science
T Haider, R Plasenzotti, H Bergmeister, G Mach, G Kleinpeter, M Aguilar-Perez, C Sherif
BACKGROUND AND PURPOSE: Thrombolytic therapy represented the gold standard for the treatment of ischemic stroke. Its drawbacks include increased bleeding risk and low recanalization rates. Mechanical thrombectomy is a new promising therapy option. Devices used for this procedure were substantially improved during recent years. New devices require extensive preclinical invivo testing. We therefore sought to translate the commonly used pig model to a simplified and cheaper rabbit model...
September 15, 2016: Journal of Neuroscience Methods
Leila Sadeghi, Farzeen Tanwir, Vahid Yousefi Babadi
Medicago sativa Linn. or alfalfa (Leguminosae) has been used traditionally as an effective cure for CNS, heart and metabolic disorders and digestive aids. Alfalfa is a resistant plant against stress due to small antioxidant molecules and enzymes. Our previous work demonstrated that 100 μg/ml of 50 nm size Fe2O3-NP causes harsh oxidative stress in HepG2 cells and 100 mg/kg of same nanoparticle causes extreme damage in rat's liver. Therefore it can be used as a useful model for invivo and invitro studies of oxidative stress...
July 18, 2016: Regulatory Toxicology and Pharmacology: RTP
Jorge C Coro, Roberto L Velasquez, Ivette M Coro, Timothy T Wheeler, Susan P McGorray, Sadao Sato
INTRODUCTION: The purpose of this study was to examine the relationship of the 3-dimensional (3D) posterior occlusal plane (POP) and the mandibular 3D spatial position. The relationship of the POP to mandibular morphology was also investigated. METHODS: Retrospective data from a convenience sample of pretreatment diagnostic cone-beam computed tomography scans were rendered using InVivo software (Anatomage, San Jose, Calif). The sample consisted of 111 subjects (51 male, 60 female) and included growing and nongrowing subjects of different races and ethnicities...
July 2016: American Journal of Orthodontics and Dentofacial Orthopedics
Lanlan Sheng, Weiguo Qu, Yang Li, Zhenyu Qu, Ji Wang
OBJECTIVE: This research aimed to analyze the three-dimensional position of mandibular canal (MC) and man of MC and its relationship with the surrounding structures dibular morphology of normal young males and females by using data from cone beam computed tomography (CBCT), as well as to provide an anatomical basis for clinical surgery of the mandible. METHODS: Normal occlusion and CBCT scans of 29 normal young people were conducted. InVivo 5 software was used to reconstruct the mandible, anchor the points, and measure the jaw shape and three-dimensional course of MC...
April 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
Fawaz Alzoubi, Nima Massoomi, Anders Nattestad
The aim of this study is to present a method, using three CAD/CAM surgical guides, to accurately obtain the desired bone reduction followed by immediate implant placements and loading for patients diagnosed with terminal dentition. Patients who had bone reduction, implants placed and immediately loaded using Anatomage Invivo 5 CAD/CAM surgical guides between the period 2013 to 2015 were evaluated retrospectively. Patients diagnosed with terminal dentition and treated using the "three-guide technique" were identified...
June 8, 2016: Journal of Oral Implantology
Fawaz Alzoubi, Nima Massoomi, Anders Nattestad
The aim of this study is to assess the accuracy of immediately placed implants using Anatomage-Invivo5 CAD/CAM surgical guides and compare it to conventional delayed implant placement protocol. Patients who had implants placed using Anatomage Invivo 5 CAD/CAM surgical guides between the period 2012 to 2015 were evaluated retrospectively. Patients who received immediate implant placements and/or delayed implant placements replacing 1-2 teeth were included in this study. Pre and post-surgical images were superimposed to evaluate deviations at the crest, apex, and angle...
June 6, 2016: Journal of Oral Implantology
Vishal Arya, Lavina Taneja, Ankit Srivastava, Swati Nandlal
INTRODUCTION: Teas is known for its anticariogenic properties and various mechanisms have been invoked to explain this effect. One such proposed mechanism is inhibition of salivary alpha amylase activity by endogenous tannins present in tea. AIM: The objective of the present study was to determine whether or not the ingestion of black tea decoction inhibits the enzyme salivary amylase and thus interferes with the release of maltose from intraoral entrapped particles of food...
March 2016: Journal of Clinical and Diagnostic Research: JCDR
Kamal Nath, Robin Victor, Subrata Naskar
It is well established fact that a combination of pharmacological therapy plus cognitive behaviour therapy (CBT) - exposure and response prevention (ERP) is considered first line for the treatment of obsessive compulsive disorder (OCD). This case presented here supports this point in unusual way of ERP administration in an atypical and resistant case of OCD proved to be beneficial over pharmacotherapy. The case was atypical in the sense that it had many overvalued ideas, superstitions and religious beliefs playing major role in its aetiology...
March 2016: Journal of Clinical and Diagnostic Research: JCDR
Mansoureh Masoudi, Sepideh Miraj, Mahmoud Rafieian-Kopaei
INTRODUCTION: There is a growing tendency towards herbal medicines for treatment of vaginitis. Antibacterial and antifungal effects of Myrtus communis L and Berberis vulgaris have been demonstrated invitro and invivo. AIM: This study aimed to compare the therapeutic effects of the vaginal gel of Berberis vulgaris 5% (in metronidazole base) and Myrtus communis L 2% (in metronidazole base) with only metronidazole vaginal gel 0.75% on bacterial vaginosis. MATERIALS AND METHODS: This study was a randomized clinical trial research on 120 married women aged 18-40 years affected by bacterial vaginosis attended for treatment to gynaecology clinic of Hajar Hospital (Shahrekord, Iran)...
March 2016: Journal of Clinical and Diagnostic Research: JCDR
Ramya Sugumar, Abhinand Ponneri Adithavarman, Anusha Dakshinamoorthi, Darling Chellathai David, Padmavathi Kannan Ragunath
INTRODUCTION: Pneumocystis jirovecii is a fungus that causes Pneumocystis pneumonia in HIV and other immunosuppressed patients. Treatment of Pneumocystis pneumonia with the currently available antifungals is challenging and associated with considerable adverse effects. There is a need to develop drugs against novel targets with minimal human toxicities. Histone Acetyl Transferase (HAT) Rtt109 is a potential therapeutic target in Pneumocystis jirovecii species. HAT is linked to transcription and is required to acetylate conserved lysine residues on histone proteins by transferring an acetyl group from acetyl CoA to form e-N-acetyl lysine...
March 2016: Journal of Clinical and Diagnostic Research: JCDR
Carlos Armando Villagomez Hoyos, Matthias Bo Stuart, Kristoffer Lindskov Hansen, Michael Bachmann Nielsen, Jorgen Arendt Jensen
This paper presents a novel approach for estimating 2-D flow angles using a high-frame-rate ultrasound method. The angle estimator features high accuracy and low standard deviation (SD) over the full 360° range. The method is validated on Field II simulations and phantom measurements using the experimental ultrasound scanner SARUS and a flow rig before being tested in vivo. An 8-MHz linear array transducer is used with defocused beam emissions. In the simulations of a spinning disk phantom, a 360° uniform behavior on the angle estimation is observed with a median angle bias of 1...
June 2016: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
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