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https://www.readbyqxmd.com/read/29132725/computational-study-of-the-role-of-fluid-content-and-flow-on-the-lumbar-disc-response-in-cyclic-compression-replication-of-in-vitro-and-in-vivo-conditions
#1
Petra Velísková, Maxim Bashkuev, Aboulfazl Shirazi-Adl, Hendrik Schmidt
The intervertebral disc viscoelastic response is governed primarily by its fluid content and flow. Invivo measurements demonstrate that the disc volume, fluid content, height and nucleus pressure completely recover during resting even after diurnal loading with twice longer duration (16 vs. 8 h). In view of much longer periods required for the recovery of disc height and pressure in vitro, concerns have been raised on the fluid inflow through the endplates that might be hampered by clogged blood vessels post mortem...
November 3, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29121225/time-dependent-glucocorticoid-administration-differently-affects-peripheral-circadian-rhythm-in-rats
#2
Tao Wu, Zhengwei Fu
There is a growing recognition that glucocorticoid (GC) acts as an internal timing signal for peripheral circadian oscillators. However, the transcription process of GC-related clock gene in the peripheral tissues is not fully understood. The present study was designed to explore the potential role of clock genes in the GC-induced peripheral circadian gene expression invivo. Real-time RT-PCR analysis indicated that the transcript levels of Per1 and Dec1 were rapidly up-regulated within 0.5 and 1 h in the heart and kidney respectively after stimulation with dexamethasone (Dex)...
November 7, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29096755/ziziphus-jujube-a-review-study-of-its-anticancer-effects-in-various-tumor-models-invitro-and-invivo
#3
S Ebrahimi, H Mollaei, R Hoshyar
The growing rate of cancer incidences and inefficiency of current therapies have made scientists to find novel agents such as medicinal plants. Ziziphus jujube is one of these plants whose pharmaceutical effects have been studied for a long time. Recent investigations have proved that bioactive compounds of Ziziphus jujube including triterpenic acids, flavonoids, cerebrosides, phenolic acids, α tocopherol, β carotene, and polysaccharides have anti-proliferative effects on several cancerous cell lines and animals...
October 31, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29059438/loss-of-dna-damage-response-in-neuroblastoma-and-utility-of-a-parp-inhibitor
#4
Masatoshi Takagi, Misa Yoshida, Yoshino Nemoto, Hiroyuki Tamaichi, Rika Tsuchida, Masafumi Seki, Kumiko Uryu, Rina Nishii, Satoshi Miyamoto, Masahiro Saito, Ryoji Hanada, Hideo Kaneko, Satoru Miyano, Keisuke Kataoka, Kenichi Yoshida, Miki Ohira, Yasuhide Hayashi, Akira Nakagawara, Seishi Ogawa, Shuki Mizutani, Junko Takita
Background: Neuroblastoma (NB) is the most common solid tumor found in children, and deletions within the 11q region are observed in 11% to 48% of these tumors. Notably, such tumors are associated with poor prognosis; however, little is known regarding the molecular targets located in 11q. Methods: Genomic alterations of ATM , DNA damage response (DDR)-associated genes located in 11q ( MRE11A, H2AFX , and CHEK1 ), and BRCA1, BARD1, CHEK2, MDM2 , and TP53 were investigated in 45 NB-derived cell lines and 237 fresh tumor samples...
November 1, 2017: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/29059021/self-assembled-supramolecular-thermoreversible-%C3%AE-cyclodextrin-ethylene-glycol-injectable-hydrogels-with-difunctional-pluronic-%C3%A2-127-as-controlled-delivery-depot-of-curcumin-development-characterization-and-in-vitro-evaluation
#5
Samiullah Khan, Muhammad Usman Minhas, Mahmood Ahmad, Mohammad Sohail
This study aimed to design a novel thermoreversible compactself-assembled host macromolecule based on β-cyclodextrin and low molecular weight ethylene glycol with a difunctional guest molecule i.e. poly (ethylene oxide)-poly (propylene oxide)-poly (ethylene oxide) tri-block co-polymer (Pluronic(®) 127) through host-guest inclusion complexation in aqueous media using cold method. The injectable thermoreversible self-assembled supramolecular hydrogel was developed to encapsulate hydrophobic curcumin as anti-malignant agent andfor controlled delivery to systemic circulation after invivo application through subcutaneous route...
October 23, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29028222/role-of-rbp2-induced-er-and-igf1r-erbb-signaling-in-tamoxifen-resistance-in-breast-cancer
#6
Hee-Joo Choi, Hyeong-Seok Joo, Hee-Young Won, Kyueng-Whan Min, Hyung-Yong Kim, Taekwon Son, Young-Ha Oh, Jeong-Yeon Lee, Gu Kong
Background: Despite the benefit of endocrine therapy, acquired resistance during or after treatment still remains a major challenge in estrogen receptor (ER)-positive breast cancer. We investigated the potential role of histone demethylase retinoblastoma-binding protein 2 (RBP2) in endocrine therapy resistance of breast cancer. Methods: Survival of breast cancer patients according to RBP2 expression was analyzed in three different breast cancer cohorts including METABRIC (n = 1980) and KM plotter (n = 1764)...
April 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28979628/determination-in-vivo-viability-of-a-transfused-platelet-product-by-corrected-count-increment-and-percentage-platelet-response
#7
Nancy Wanjiru Thuku, Kibet Shikuku, Amos Mbugua
INTRODUCTION: For many years, platelet concentrates have been used for the prevention as well as treatment of bleeding disorders, especially in those patients with haematological problems involving platelet disorders as well as refractoriness, In addition, platelet concentrates (PCs) have been widely used to support patients undergoing bone marrow transplantation or who are receiving myelotoxic treatments. The aim of this study was to determine the quality of platelet concentrates by assessing platelet counts, volume, pH changes, swirling, residue of the red blood cells and white blood cell counts...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28962526/from-the-cover-metabolism-modulation-in-different-organs-by-silver-nanoparticles-an-nmr-metabolomics-study-of-a-mouse-model
#8
Ivana Jarak, Joana Carrola, António S Barros, Ana M Gil, Maria de Lourdes Pereira, Maria Luisa Corvo, Iola F Duarte
Although silver nanoparticles (AgNPs) are widely disseminated and show great potential in the biomedical field, there is a recognized need to better understand their action at the metabolic and functional levels. In this work, we have used NMR metabolomics, together with conventional clinical chemistry and histological examination, to characterize multi-organ and systemic metabolic responses to AgNPs intravenously administered to mice at 8 mg/kg body weight (a dose not eliciting overt toxicity). The major target organs of AgNPs accumulation, liver and spleen, showed the greatest metabolic changes, in a clear 2-stage response...
October 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28962518/from-the-cover-an-animal-free-in-vitro-three-dimensional-testicular-cell-coculture-model-for-evaluating-male-reproductive-toxicants
#9
Lei Yin, Hongye Wei, Shenxuan Liang, Xiaozhong Yu
Primary testicular cell coculture model has been used to evaluate testicular abnormalities during development, and was able to identify the testicular toxicity of phthalates. However, the primary testicular cell coculture model has disadvantages in employing animals for the isolation of testicular cells, and the complicated isolation procedure leads to inconsistent results. We developed an invitro testicular coculture model from rodent testicular cell lines, including spermatogonial cells, Sertoli cells, and Leydig cells with specified cell density and extracellular matrix (ECM) composition...
October 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28936163/quercetin-declines-apoptosis-ameliorates-mitochondrial-function-and-improves-retinal-ganglion-cell-survival-and-function-in-in-vivo-model-of-glaucoma-in-rat-and-retinal-ganglion-cell-culture-in-vitro
#10
Feng-Juan Gao, Sheng-Hai Zhang, Ping Xu, Bo-Qi Yang, Rong Zhang, Yun Cheng, Xu-Jiao Zhou, Wan-Jing Huang, Min Wang, Jun-Yi Chen, Xing-Huai Sun, Ji-Hong Wu
Glaucoma is a progressive neuropathy characterized by the loss of retinal ganglion cells (RGCs). Strategies that delay or halt RGC loss have been recognized as potentially beneficial for rescuing vision in glaucoma patients. Quercetin (Qcn) is a natural and important dietary flavonoid compound, widely distributed in fruits and vegetables. Mounting evidence suggests that Qcn has numerous neuroprotective effects. However, whether Qcn exerts neuroprotective effects on RGC in glaucoma is poorly understood. In this study, we investigated the protective effect of Qcn against RGC damage in a rat chronic ocular hypertension (COHT) model invivo and hypoxia-induced primary cultured RGC damage in vitro, and we further explored the underlying neuroprotective mechanisms...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28932139/predictability-of-bone-density-at-posterior-mandibular-implant-sites-using-cone-beam-computed-tomography-intensity-values
#11
Mustafa Alkhader, Malik Hudieb, Yousef Khader
OBJECTIVE: The aim of this study was to investigate the predictability of bone density at posterior mandibular implant sites using cone-beam computed tomography (CBCT) intensity values. MATERIALS AND METHODS: CBCT cross-sectional images for 436 posterior mandibular implant sites were selected for the study. Using Invivo software (Anatomage, San Jose, California, USA), two observers classified the bone density into three categories: low, intermediate, and high, and CBCT intensity values were generated...
July 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28903500/intrinsic-xenobiotic-metabolizing-enzyme-activities-in-early-life-stages-of-zebrafish-danio-rerio
#12
Jens C Otte, Bernadette Schultz, Daniela Fruth, Eric Fabian, Bennard van Ravenzwaay, Björn Hidding, Edward R Salinas
Early life stages of zebrafish (Danio rerio, zf) are gaining attention as an alternative invivo test system for drug discovery, early developmental toxicity screenings and chemical testing in ecotoxicological and toxicological testing strategies. Previous studies have demonstrated transcriptional evidence for xenobiotic metabolizing enzymes (XME) during early zf development. However, elaborate experiments on XME activities during development are incomplete. In this work, the intrinsic activities of representative phase I and II XME were monitored by transformation of putative zf model substrates analyzed using photometry and high pressure liquid chromatography techniques...
September 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28903498/from-the-cover-lifelong-exposure-of-c57bl-6n-male-mice-to-bisphenol-a-or-bisphenol-s-reduces-recovery-from-a-myocardial-infarction
#13
Amanda Kasneci, Jun Seong Lee, Tae Jin Yun, Jijun Shang, Shaun Lampen, Tamar Gomolin, Cheolho C Cheong, Lorraine E Chalifour
Bisphenol A (BPA) leaches from plastics to contaminate foodstuffs. Analogs, such as bisphenol S (BPS), are now used increasingly in manufacturing. Greater BPA exposure has been correlated with exacerbation of cardiovascular disease, including myocardial infarction (MI). To test the hypothesis that bisphenol exposure impairs cardiac healing, we exposed C57bl/6n mice to water containing 25ng/ml BPA or BPS from conception and surgically induced an MI in adult male progeny. Increased early death and cardiac dilation, and reduced cardiac function were found post-MI in BPA- and BPS-exposed mice...
September 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28903491/use-of-rat-primary-mesenteric-cells-for-the-prediction-of-pde4-inhibitor-drug-induced-vascular-injury
#14
Mark M Gosink, Robert E Chapin, Dean Wilkie, Scott Davenport, Steven W Kumpf, Bradley E Enerson, Christopher Houle, Petra Koza-Taylor, Todd A Wisialowski, Michael P Lawton
Drug-induced vascular injury (DIVI) in preclinical studies can delay, if not terminate, a drug development program. Clinical detection of DIVI can be very difficult as there are no definitive biomarkers known to reliably detect this disorder in all instances. The preclinical identification of DIVI requires detailed microscopic examination of a wide range of tissues although one of the most commonly affected areas in rats is the mesenteric vasculature. The reason for this predisposition of mesenteric arteries in rats as well as the exact mechanism and cell types involved in the initial development of these lesions have not been fully elucidated...
September 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28903487/from-the-cover-arsenic-induces-hippocampal-neuronal-apoptosis-and-cognitive-impairments-via-an-up-regulated-bmp2-smad-dependent-reduced-bdnf-trkb-signaling-in-rats
#15
Rukmani Pandey, Vipin Rai, Juhi Mishra, Kapil Mandrah, Somendu Kumar Roy, Sanghamitra Bandyopadhyay
Arsenic promotes hippocampal neuronal damage inducing cognitive impairments. However, mechanism arbitrating arsenic-mediated cognitive deficits remains less-known. Here, we identified that chronic exposure to environmentally relevant doses of arsenic increased apoptosis, characterized by caspase-3 activation, poly(ADP-ribose) polymerase cleavage and Terminal deoxynucleotidyl transferase dUTP nick-end labeling of rat hippocampal neurons, marked by NeuN. Investigating apoptotic mechanism through invivo and invitro studies revealed that arsenic promoted bone morphogenetic protein-2 (BMP2) expression, supported by increased BMP-receptor2 (BMPR2) and p-Smad1/5 in hippocampal neurons...
September 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28903484/novel-role-of-fbxw7-circular-rna-in-repressing-glioma-tumorigenesis
#16
Yibing Yang, Xinya Gao, Maolei Zhang, Sheng Yan, Chengjun Sun, Feizhe Xiao, Nunu Huang, Xuesong Yang, Kun Zhao, Huangkai Zhou, Suyun Huang, Bo Xie, Nu Zhang
Background: Circular RNAs (circRNAs) are RNA transcripts that are widespread in the eukaryotic genome. Recent evidence indicates that circRNAs play important roles in tissue development, gene regulation, and carcinogenesis. However, whether circRNAs encode functional proteins remains elusive, although translation of several circRNAs was recently reported. Methods: CircRNA deep sequencing was performed by using 10 pathologically diagnosed glioblastoma samples and their paired adjacent normal brain tissues...
March 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28881206/polyglycerolated-nanocarriers-with-increased-ligand-multivalency-for-enhanced-invivo-therapeutic-efficacy-of-paclitaxel
#17
Trang Huyen Le Kim, Jeong Heon Yu, Hwiseok Jun, Moon Young Yang, Mi-Jin Yang, Jae-Woo Cho, Jin Woong Kim, Jee Seon Kim, Yoon Sung Nam
Despite the excellent biocompatibility and antifouling effect of poly(ethylene glycol) (PEG), the high steric hindrance, limited chemical functionality, and low ligand multivalency of PEGylated nanocarriers often lead to inefficient cell targeting and intracellular trafficking. Hence, a new structure of hydrophilic corona allowing a higher ligand density without loss of excellent biocompatibility is highly desirable. Here we introduce tumor-targeted polyglycerolated (PGylated) nanocarriers that dramatically enhance the in vivo therapeutic efficacy of incorporated paclitaxel simply by increasing the surface density of hydrophobic tumor-targeting ligands...
August 29, 2017: Biomaterials
https://www.readbyqxmd.com/read/28811325/ppar%C3%AE-ligand-induced-annexin-a1-expression-determines-chemotherapy-response-via-deubiquitination-of-death-domain-kinase-rip-in-triple-negative-breast-cancers
#18
Luxi Chen, Yi Yuan, Shreya Kar, Madhu M Kanchi, Suruchi Arora, Ji E Kim, Pei F Koh, Einas Yousef, Ramar P Samy, Muthu K Shanmugam, Tuan Z Tan, Sung W Shin, Frank Arfuso, Han M Shen, Henry Yang, Boon C Goh, Joo I Park, Louis Gaboury, Peter E Lobie, Gautam Sethi, Lina Hk Lim, Alan P Kumar
Metastatic breast cancer is still remain incurable so far, new specifically targeted and more effective therapies for triple negative breast cancer (TNBC) are required in the clinic. In this study, our clinical data has established that basal and claudin-low subtypes of breast cancer (TNBC types) express significantly higher levels of Annexin A1 (ANXA1) with poor survival outcomes. Using human cancer cell lines which model the TNBC subtype, we observed a strong positive correlation between expression of ANXA1 and Peroxisome Proliferator-Activated Receptor gamma (PPARγ)...
August 15, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28679777/efficacy-of-onalespib-a-long-acting-second-generation-hsp90-inhibitor-as-a-single-agent-and-in-combination-with-temozolomide-against-malignant-gliomas
#19
Alessandro Canella, Alessandra M Welker, Ji Young Yoo, Jihong Xu, Fazly S Abas, Divya Kesanakurti, Prabakaran Nagarajan, Christine E Beattie, Erik P Sulman, Joseph Liu, Joy Gumin, Frederick F Lang, Metin N Gurcan, Balveen Kaur, Deepa Sampath, Vinay K Puduvalli
Purpose: HSP90, a highly conserved molecular chaperone that regulates the function of several oncogenic client proteins, is altered in glioblastoma. However, HSP90 inhibitors currently in clinical trials are short-acting, have unacceptable toxicities, or are unable to cross the blood-brain barrier (BBB). We examined the efficacy of onalespib, a potent, long-acting novel HSP90 inhibitor as a single agent and in combination with temozolomide (TMZ) against gliomas in vitro and in vivoExperimental Design: The effect of onalespib on HSP90, its client proteins, and on the biology of glioma cell lines and patient-derived glioma-initiating cells (GSC) was determined...
October 15, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28671655/switchable-acoustic-and-optical-resolution-photoacoustic-microscopy-for-in-vivo-small-animal-blood-vasculature-imaging
#20
Mohesh Moothanchery, Arunima Sharma, Manojit Pramanik
Photoacoustic microscopy (PAM) is a fast-growing invivo imaging modality that combines both optics and ultrasound, providing penetration beyond the optical mean free path (~1 mm in skin) with high resolution. By combining optical absorption contrast with the high spatial resolution of ultrasound in a single modality, this technique can penetrate deep tissues. Photoacoustic microscopy systems can have either a low acoustic resolution and probe deeply or a high optical resolution and probe shallowly. It is challenging to achieve high spatial resolution and large depth penetration with a single system...
June 26, 2017: Journal of Visualized Experiments: JoVE
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