R J Bevacqua, R Fernandez-Martín, V Savy, N G Canel, M I Gismondi, W A Kues, D F Carlson, S C Fahrenkrug, H Niemann, O A Taboga, S Ferraris, D F Salamone
The recently developed engineered nucleases, such as zinc-finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease (Cas) 9, provide new opportunities for gene editing in a straightforward manner. However, few reports are available regarding CRISPR application and efficiency in cattle. Here, the CRISPR/Cas9 system was used with the aim of inducing knockout and knock-in alleles of the bovine PRNP gene, responsible for mad cow disease, both in bovine fetal fibroblasts and in IVF embryos...
November 2016: Theriogenology