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Human skin substitute

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https://www.readbyqxmd.com/read/28412341/chitosan-a-potential-biopolymer-for-wound-management
#1
REVIEW
Ijaz Bano, Muhammad Arshad, Tariq Yasin, Muhammad Afzal Ghauri, Muhammad Younus
It has been seen that slow healing and non-healing wounds conditions are treatable but still challenging to humans. Wound dressing usually seeks for biocompatible and biodegradable recipe. Natural polysaccharides like chitosan have been examined for its antimicrobial and healing properties on the basis of its variation in molecular weight and degree of deacetylation. Chitosan adopts some vital characteristics for treatment of various kinds of wounds which include its bonding nature, antifungal, bactericidal and permeability to oxygen...
April 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28403359/oral-mucosa-an-alternative-epidermic-cell-source-to-develop-autologous-dermal-epidermal-substitutes-from-diabetic-subjects
#2
Daniela Guzmán-Uribe, Keila Neri Alvarado-Estrada, Mauricio Pierdant-Pérez, Bertha Torres-Álvarez, Jesus Martin Sánchez-Aguilar, Raúl Rosales-Ibáñez
Objective: The aim of this study was to obtain autologous dermal-epidermal skin substitutes from oral mucosa from diabetic subjects as a first step towards a possible clinical application for cases of diabetic foot. Material and Methods: Oral mucosa was obtained from diabetic and healthy subjects (n=20 per group). Epidermal cells were isolated and cultured using autologous fibrin to develop dermal-epidermal in vitro substitutes by the air-liquid technique with autologous human serum as a supplement media...
March 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28394544/fibrin-gel-as-a-scaffold-for-skin-substitute-production-and-clinical-experience
#3
(no author information available yet)
The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaff olds were prepared from commercial fibrin glue kits. Human fibroblasts were cultured in fibrin gel. Human keratinocytes were seeded on the top of the gel. Viability of cells was determined fluorimetrically. Scanning electron microscope and immunocytochemistry analysis of cultured cells were performed...
June 2016: Acta Clinica Croatica
https://www.readbyqxmd.com/read/28361280/temporary-skin-grafts-based-on-hybrid-graphene-oxide-natural-biopolymer-nanofibers-as-effective-wound-healing-substitutes-pre-clinical-and-pathological-studies-in-animal-models
#4
N Mahmoudi, N Eslahi, A Mehdipour, M Mohammadi, M Akbari, A Samadikuchaksaraei, A Simchi
In recent years, temporary skin grafts (TSG) based on natural biopolymers modified with carbon nanostructures have received considerable attention for wound healing. Developments are required to improve physico-mechanical properties of these materials to match to natural skins. Additionally, in-deep pre-clinical examinations are necessary to ensure biological performance and toxicity effect in vivo. In the present work, we show superior acute-wound healing effect of graphene oxide nanosheets embedded in ultrafine biopolymer fibers (60 nm) on adult male rats...
May 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28324936/a-preliminary-study-on-multi-wavelength-ppg-based-pulse-transit-time-detection-for-cuffless-blood-pressure-measurement
#5
Jing Liu, Yuan-Ting Zhang, Xiao-Rong Ding, Wen-Xuan Dai, Ni Zhao
Pulse transit time (PTT) has been widely studied as an index of blood pressure (BP) changes. In recent years, some prototypes of PTT-based wearable BP measurement devices have been developed, which can relieve users from the discomfort caused by the inflating cuff used in auscultatory and oscillometric BP measurement techniques. However, in the common practice for PTT detection, multi-site sensor implementation on human body is required, making it difficult for the integration of wearable devices. Since multi-wavelength (MW) photoplethysmogram (PPG) signals carry blood pulsation information of different blood vessels embedded in different skin depths, the time difference between different wavelength PPG signals collected at the same body site can be treated as a special PTT on a short length of blood vessels beneath the skin...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28323389/atomic-force-microscopy-in-the-production-of-a-biovital-skin-graft-based-on-human-acellular-dermal-matrix-produced-in-house-and-in-vitro-cultured-human-fibroblasts
#6
Wojciech Łabuś, Justyna Glik, Agnieszka Klama-Baryła, Diana Kitala, Małgorzata Kraut, Mariusz Maj, Mariusz Nowak, Marcelina Misiuga, Andrzej Marcinkowski, Barbara Trzebicka, Ryszard Poloczek, Marek Kawecki
The most efficient method in III° burn treatment is the use of the autologous split thickness skin grafts that were donated from undamaged body area. The main limitation of this method is lack of suitable donor sites. Tissue engineering is a useful tool to solve this problem. The goal of this study was to find the most efficient way of producing biovital skin substitute based on in house produced acellular dermal matrix ADM and in vitro cultured fibroblasts. Sixty samples of sterilized human allogeneic skin (that came from 10 different donors) were used to examine the influence of decellularizing substances on extracellular matrix and clinical usefulness of the test samples of allogeneic human dermis...
March 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28293507/soluble-epoxide-hydrolase-inhibition-and-epoxyeicosatrienoic-acid-treatment-improve-vascularization-of-engineered-skin-substitutes
#7
Dorothy M Supp, Jennifer M Hahn, Kevin L McFarland, Kelly A Combs, Kin Sing Stephen Lee, Bora Inceoglu, Debin Wan, Steven T Boyce, Bruce D Hammock
BACKGROUND: Autologous engineered skin substitutes comprised of keratinocytes, fibroblasts, and biopolymers can serve as an adjunctive treatment for excised burns. However, engineered skin lacks a vascular plexus at the time of grafting, leading to slower vascularization and reduced rates of engraftment compared with autograft. Hypothetically, vascularization of engineered skin grafts can be improved by treatment with proangiogenic agents at the time of grafting. Epoxyeicosatrienoic acids (EETs) are cytochrome P450 metabolites of arachidonic acid that are inactivated by soluble epoxide hydrolase (sEH)...
December 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28258125/a-drosophila-model-of-dominant-inclusion-body-myopathy-3-shows-diminished-myosin-kinetics-that-reduce-muscle-power-and-yield-myofibrillar-defects
#8
Jennifer A Suggs, Girish C Melkani, Bernadette M Glasheen, Mia M Detor, Anju Melkani, Nathan P Marsan, Douglas M Swank, Sanford I Bernstein
Inclusion body myopathy type 3 (IBM-3) patients display congenital joint contractures with early-onset muscle weakness that becomes more severe in adults. The disease arises from an autosomal dominant point mutation causing an E706K substitution in myosin heavy chain type IIa. We previously expressed the corresponding myosin mutation (E701K) in homozygous Drosophila indirect flight muscles and recapitulated the myofibrillar degeneration and inclusion bodies observed in the human disease. We also found that purified E701K myosin has dramatically reduced actin-sliding velocity and ATPase levels...
March 3, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28226573/a-preliminary-study-on-multi-wavelength-ppg-based-pulse-transit-time-detection-for-cuffless-blood-pressure-measurement
#9
Jing Liu, Yuan-Ting Zhang, Xiao-Rong Ding, Wen-Xuan Dai, Ni Zhao, Jing Liu, Yuan-Ting Zhang, Xiao-Rong Ding, Wen-Xuan Dai, Ni Zhao, Wen-Xuan Dai, Yuan-Ting Zhang, Xiao-Rong Ding, Ni Zhao, Jing Liu
Pulse transit time (PTT) has been widely studied as an index of blood pressure (BP) changes. In recent years, some prototypes of PTT-based wearable BP measurement devices have been developed, which can relieve users from the discomfort caused by the inflating cuff used in auscultatory and oscillometric BP measurement techniques. However, in the common practice for PTT detection, multi-site sensor implementation on human body is required, making it difficult for the integration of wearable devices. Since multi-wavelength (MW) photoplethysmogram (PPG) signals carry blood pulsation information of different blood vessels embedded in different skin depths, the time difference between different wavelength PPG signals collected at the same body site can be treated as a special PTT on a short length of blood vessels beneath the skin...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28223803/protein-nanocoatings-on-synthetic-polymeric-nanofibrous-membranes-designed-as-carriers-for-skin-cells
#10
Marketa Bacakova, Julia Pajorova, Denisa Stranska, Daniel Hadraba, Frantisek Lopot, Tomas Riedel, Eduard Brynda, Margit Zaloudkova, Lucie Bacakova
Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28209294/sulfated-glycosaminoglycans-and-non-classically-secreted-proteins-basic-fgf-and-epimorphin-coordinately-regulate-tgf-%C3%AE-induced-cell-behaviors-of-human-scar-dermal-fibroblasts
#11
Tomoatsu Horigome, Shinya Takumi, Kota Shirai, Takumi Kido, Natsumi Hagiwara-Chatani, Ayumi Nakashima, Naoki Adachi, Hiroko Yano, Yohei Hirai
BACKGROUND: Upon skin injuries, dermal fibroblasts actively produce transforming growth factor-β (TGF-β), which leads to the formation of α-smooth muscle actin (αSMA)-positive granulation tissues. The hyperplasia or incomplete regression of these tissues subsequently causes scar formation in the skin, where sulfated glycosaminoglycans (GAGs), side chains of unique proteoglycans, are supposed to play important roles. OBJECTIVE: The aim of this study is to clarify the effects of sulfated GAGs on dermal cell behaviors triggered by the TGF-β signaling, along with its possible regulators basic fibroblast growth factor (bFGF) and cell surface epimorphin...
February 3, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28194314/evolutionary-and-functional-implications-of-hypervariable-loci-within-the-skin-virome
#12
Geoffrey D Hannigan, Qi Zheng, Jacquelyn S Meisel, Samuel S Minot, Frederick D Bushman, Elizabeth A Grice
Localized genomic variability is crucial for the ongoing conflicts between infectious microbes and their hosts. An understanding of evolutionary and adaptive patterns associated with genomic variability will help guide development of vaccines and antimicrobial agents. While most analyses of the human microbiome have focused on taxonomic classification and gene annotation, we investigated genomic variation of skin-associated viral communities. We evaluated patterns of viral genomic variation across 16 healthy human volunteers...
2017: PeerJ
https://www.readbyqxmd.com/read/28094220/molecular-docking-discovery-synthesis-and-pharmacological-properties-of-new-6-substituted-2-3-phenoxyphenyl-4-phenyl-quinoline-derivatives-an-approach-to-developing-potent-dna-gyrase-inhibitors-antibacterial-agents
#13
Manikandan Alagumuthu, Sivakumar Arumugam
Synthesis and molecular validation of 6-substituted-2-(3-phenoxyphenyl)-4-phenylquinoline derivatives (4a-h) as antibacterial/DNA gyrase inhibitors reported. Primarily, 6-substituted-2-(3-phenoxyphenyl)-4-phenylquinoline derivatives were docked into the active sites of DNA gyrase A&B, to ensure the binding mode of the compounds, and the results were superior on DNA gyrase A over DNA gyrase B. Based on this, S. aureus DNA gyrase A assay was proposed and executed. Most prominent DNA gyrase inhibition showed by 6-fluoro-2-(3-phenoxyphenyl)-4-phenylquinoline (4c), IC50 0...
January 6, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28090513/an-update-and-review-of-cell-based-wound-dressings-and-their-integration-into-clinical-practice
#14
REVIEW
Austin Pourmoussa, Daniel J Gardner, Maxwell B Johnson, Alex K Wong
Chronic wounds affect over 4 million individuals and pose a significant burden to the US healthcare system. Diabetes, venous stasis, radiation or paralysis are common risk factors for chronic wounds. Unfortunately, the current standard of care (SOC) has a high relapse rate and these wounds continue to adversely affect patients' quality of life. Fortunately, advances in tissue engineering have allowed for the development of cell-based wound dressings that promote wound healing by improving cell migration and differentiation...
December 2016: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28087229/disease-associated-mutations-identify-a-novel-region-in-human-sting-necessary-for-the-control-of-type-i-interferon-signaling
#15
Isabelle Melki, Yoann Rose, Carolina Uggenti, Lien Van Eyck, Marie-Louise Frémond, Naoki Kitabayashi, Gillian I Rice, Emma M Jenkinson, Anaïs Boulai, Nadia Jeremiah, Marco Gattorno, Sefano Volpi, Olivero Sacco, Suzanne W J Terheggen-Lagro, Harm A W M Tiddens, Isabelle Meyts, Marie-Anne Morren, Petra De Haes, Carine Wouters, Eric Legius, Anniek Corveleyn, Frederic Rieux-Laucat, Christine Bodemer, Isabelle Callebaut, Mathieu P Rodero, Yanick J Crow
BACKGROUND: Gain-of-function mutations in transmembrane protein 173 (TMEM173) encoding stimulator of interferon genes (STING) underlie a recently described type I interferonopathy called STING-associated vasculopathy with onset in infancy (SAVI). OBJECTIVES: We sought to define the molecular and cellular pathology relating to 3 individuals variably exhibiting the core features of the SAVI phenotype including systemic inflammation, destructive skin lesions, and interstitial lung disease...
January 3, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28060331/biomechanical-characterization-of-human-soft-tissues-using-indentation-and-tensile-testing
#16
Michelle Griffin, Yaami Premakumar, Alexander Seifalian, Peter Edward Butler, Matthew Szarko
Regenerative medicine aims to engineer materials to replace or restore damaged or diseased organs. The mechanical properties of such materials should mimic the human tissues they are aiming to replace; to provide the required anatomical shape, the materials must be able to sustain the mechanical forces they will experience when implanted at the defect site. Although the mechanical properties of tissue-engineered scaffolds are of great importance, many human tissues that undergo restoration with engineered materials have not been fully biomechanically characterized...
December 13, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28042321/pre-vascularization-enhances-therapeutic-effects-of-human-mesenchymal-stem-cell-sheets-in-full-thickness-skin-wound-repair
#17
Lei Chen, Qi Xing, Qiyi Zhai, Mitchell Tahtinen, Fei Zhou, Lili Chen, Yingbin Xu, Shaohai Qi, Feng Zhao
Split thickness skin graft (STSG) implantation is one of the standard therapies for full thickness wound repair when full thickness autologous skin grafts (FTG) or skin flap transplants are inapplicable. Combined transplantation of STSG with dermal substitute could enhance its therapeutic effects but the results remain unsatisfactory due to insufficient blood supply at early stages, which causes graft necrosis and fibrosis. Human mesenchymal stem cell (hMSC) sheets are capable of accelerating the wound healing process...
2017: Theranostics
https://www.readbyqxmd.com/read/28041746/evaluation-of-cultured-human-dermal-and-dermo-epidermal-substitutes-focusing-on-extracellular-matrix-components-comparison-of-protein-and-rna-analysis
#18
Corien Oostendorp, Sarah Meyer, Monia Sobrio, Joyce van Arendonk, Ernst Reichmann, Willeke F Daamen, Toin H van Kuppevelt
Treatment of full-thickness skin defects with split-thickness skin grafts is generally associated with contraction and scar formation and cellular skin substitutes have been developed to improve skin regeneration. The evaluation of cultured skin substitutes is generally based on qualitative parameters focusing on histology. In this study we focused on quantitative evaluation to provide a template for comparison of human bio-engineered skin substitutes between clinical and/or research centers, and to supplement histological data...
December 29, 2016: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28040611/factors-affecting-the-mechanical-behavior-of-collagen-hydrogels-for-skin-tissue-engineering
#19
Marco Pensalfini, Alexander E Ehret, Silvia Stüdeli, Daniela Marino, Andres Kaech, Ernst Reichmann, Edoardo Mazza
The effect of the production factors yielding a functional dermal substitute was investigated by means of monotonic and cyclic uniaxial tensile tests, as well as electron microscopy visualizations. The role of (i) plastic compression, (ii) product incubation, and (iii) cell permanence in the collagenous matrix in order to achieve a skin-like behavior were characterized in terms of material and structural stiffness, in-plane kinematics, and cyclic response, as well as pore size and network density. The plastic compression resulted in a denser and stiffer material, while no corresponding change was observed in the behavior of the entire structure...
December 9, 2016: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28024598/design-and-characterization-of-3d-hybrid-collagen-matrixes-as-a-dermal-substitute-in-skin-tissue-engineering
#20
Giriprasath Ramanathan, Sivakumar Singaravelu, Thangavelu Muthukumar, Sitalakshmi Thyagarajan, Paramasivan Thirumalai Perumal, Uma Tiruchirapalli Sivagnanam
The highly interconnected porous dressing material was fabricated with the utilization of novel collagen (COL-SPG) for the efficient healing of the wound. Herein, we report the fabrication of 3D collagen impregnated with bioactive extract (COL-SPG-CPE) to get rid of infection at the wound site. The resultant 3D collagen matrix was characterized physiochemically using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and mechanical property. The dressing substrate possesses the high swelling ability, increase in the porosity, in vitro enzymatic degradability and antibacterial property...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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