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Human skin substitute

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https://www.readbyqxmd.com/read/27903244/cryopreservation-of-dermal-fibroblasts-and-keratinocytes-in-hydroxyethyl-starch-based-cryoprotectants
#1
Yahaira Naaldijk, Adiv A Johnson, Annett Friedrich-Stöckigt, Alexandra Stolzing
BACKGROUND: Preservation of human skin fibroblasts and keratinocytes is essential for the creation of skin tissue banks. For successful cryopreservation of cells, selection of an appropriate cryoprotectant agent (CPA) is imperative. The aim of this study was to identify CPAs that minimize toxic effects and allow for the preservation of human fibroblasts and keratinocytes in suspension and in monolayers. RESULTS: We cryopreserved human fibroblasts and keratinocytes with different CPAs and compared them to fresh, unfrozen cells...
December 1, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27878297/hydroxyproline-containing-collagen-peptide-derived-from-the-skin-of-the-alaska-pollack-inhibits-hiv-1-infection
#2
In Seung Jang, Sun Joo Park
The human immunodeficiency virus (HIV) is a lentivirus that results in acquired immunodeficiency syndrome (AIDS). HIV treatment involving chemical therapeutic agents has improved the quality of life of HIV/AIDS patients. The present study demonstrates that a hydroxyproline-containing marine collagen peptide (APHCP) derived from Alaska pollack inhibits HIV‑1 infection in the MT-4 human T cell‑line. APHCP inhibited HIV-1IIIB-induced cell lysis, syncytia formation, reverse transcriptase activity and viral p24 production at non‑cytotoxic concentrations; however, APHCP did not inhibit HIV‑2ROD infection in MT‑4 cells...
November 16, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27872793/improved-methods-to-produce-tissue-engineered-skin-substitutes-suitable-for-the-permanent-closure-of-full-thickness-skin-injuries
#3
Danielle Larouche, Laurence Cantin-Warren, Maxime Desgagné, Rina Guignard, Israël Martel, Akram Ayoub, Amélie Lavoie, Robert Gauvin, François A Auger, Véronique J Moulin, Lucie Germain
There is a clinical need for skin substitutes to replace full-thickness skin loss. Our group has developed a bilayered skin substitute produced from the patient's own fibroblasts and keratinocytes referred to as Self-Assembled Skin Substitute (SASS). After cell isolation and expansion, the current time required to produce SASS is 45 days. We aimed to optimize the manufacturing process to standardize the production of SASS and to reduce production time. The new approach consisted in seeding keratinocytes on a fibroblast-derived tissue sheet before its detachment from the culture plate...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27817210/evaluation-of-a-new-topical-skin-protectant-rd1433-for-the-prevention-and-treatment-of-incontinence-associated-dermatitis
#4
Hazem Matar, Joanne Larner, Vanessa Viegas, Sneha Kansagra, Karen L Atkinson, Satyajit Shetage, John T Skamarauskas, Baveetharan Theivendran, Virginia S Goldman, Robert P Chilcott
CONTEXT: Incontinence-associated dermatitis (IAD) is a type of moisture-associated dermatitis caused by repeated skin exposure to urine or stool. A product that could mitigate such symptoms would have a significant impact on cost of care and patients' quality of life. OBJECTIVE: This study compared the clinical efficacy of RD1433 and a comparator product (Vaseline®) in preventing and treating experimental IAD skin lesions. MATERIALS AND METHODS: For the "prevention" part of the study, skin sites in eight human volunteers were treated daily for five days with either RD1433 or Vaseline® immediately prior to synthetic urine exposure...
November 6, 2016: Cutaneous and Ocular Toxicology
https://www.readbyqxmd.com/read/27795423/dysregulated-glycoprotein-b-mediated-cell-cell-fusion-disrupts-varicella-zoster-virus-and-host-gene-transcription-during-infection
#5
Stefan L Oliver, Edward Yang, Ann M Arvin
: The highly conserved herpesvirus glycoprotein complex, gB/gH-gL, mediates membrane fusion during virion entry and cell-cell fusion. Varicella-zoster virus (VZV) characteristically forms multi-nucleated cells, or syncytia, during the infection of human tissues but little is known about this process. The cytoplasmic domain of VZV gB (gBcyt) has been implicated in cell-cell fusion regulation because a gB[Y881F] substitution causes hyperfusion. The gBcyt regulation is necessary for VZV pathogenesis as the hyperfusogenic mutant gB[Y881F] is severely attenuated in human skin xenografts...
October 26, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27775538/a-system-for-electrotactile-feedback-using-electronic-skin-and-flexible-matrix-electrodes-experimental-evaluation
#6
Marta Franceschi, Lucia Seminara, Strahinja Dosen, Matjia Strbac, Maurizio Valle, Dario Farina
Myoelectric prostheses are successfully controlled using muscle electrical activity, thereby restoring lost motor functions. However, the somatosensory feedback from the prosthesis to the user is still missing. The sensory substitution methods described in the literature comprise mostly simple position and force sensors combined with discrete stimulation units. The present study describes a novel system for sophisticated electrotactile feedback integrating advanced distributed sensing (electronic skin) and stimulation (matrix electrodes)...
October 19, 2016: IEEE Transactions on Haptics
https://www.readbyqxmd.com/read/27721966/human-split-thickness-skin-allograft-from-brain-dead-donors
#7
A Khodadadi, O Olang, A Makhllough, B Nozary Heshmati, F Azmoudeh Ardalan, S A Tavakoli
BACKGROUND: Looking for an appropriate skin substitute for temporary and permanent coverage of wounds remains one of the main obstacles of medical researchers. OBJECTIVE: To investigate the rate of inflammation, symbiosis, and survival of grafted allograft skin from brain-dead donors (BDDs) in rabbits. METHODS: After receiving negative serologic tests of BDDs, we prepared partial thickness skin grafts. They were then used in treating wounds of 5 rabbits in comparison with split-thickness skins taken from cardiac dead donors...
2016: International Journal of Organ Transplantation Medicine
https://www.readbyqxmd.com/read/27667541/production-and-characterization-of-polycaprolactone-hyaluronic-acid-chitosan-zein-electrospun-bilayer-nanofibrous-membrane-for-tissue-regeneration
#8
Daniela R Figueira, Sónia P Miguel, Kevin D de Sá, Ilídio J Correia
A bilayered electrospun membrane was produced in this study, using the electrospinning technique, to be applied as a skin substitute. The upper layer of the membrane was comprised by hyaluronic acid and polycaprolactone in order to provide mechanical support and also to act as a physical barrier against external threats. Chitosan and zein were used to produce the bottom layer that was loaded with salicylic acid, in order to confer anti-inflammatory and antimicrobial activity to this layer. The physicochemical properties of the membranes were determined and the obtained results showed that the produced electrospun membrane display an ideal porosity, appropriate mechanical properties, controlled water loss and a suitable salicylic acid release profile...
September 22, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27665246/in-view-of-standardization-part-2-management-of-challenges-in-the-initial-treatment-of-burn-patients-in-burn-centers-in-germany-austria-and-switzerland
#9
Benjamin Ziegler, Christoph Hirche, Johannes Horter, Jurij Kiefer, Paul Alfred Grützner, Thomas Kremer, Ulrich Kneser, Matthias Münzberg
INTRODUCTION: Initial therapy of severe burns in specialized burn trauma centers is a challenging task faced by the treating multi-professional and interdisciplinary team. A lack of consistent operating procedures and varying structural conditions was recently demonstrated in preliminary data of our group. These results raised the question on how specific treatment measures in acute burn care are met in the absence of standardized guidelines. MATERIAL AND METHODS: A specific questionnaire containing 57 multiple-choice questions was sent to all 22 major burn centers in Germany, Austria and Switzerland...
September 21, 2016: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/27651371/successful-grafting-of-tissue-engineered-fetal-skin
#10
L Mazzone, M Pratsinis, L Pontiggia, E Reichmann, M Meuli
PURPOSE: Fetal repair of spina bifida results in improved outcomes and has therefore become a standard clinical procedure in some highly specialized centers. However, optimization of the procedure technique and timing is needed. Both might be achieved by facilitating the procedure using laboratory-grown fetal skin substitutes. The aim of this study was therefore to test in vivo the suitability of such a fetal skin substitute for an in utero application. METHODS: Collagen-based hydrogels containing fetal ovine fibroblasts were seeded with fetal ovine keratinocytes and transplanted on immuno-incompetent nu/nu rats...
September 20, 2016: Pediatric Surgery International
https://www.readbyqxmd.com/read/27651370/myelinated-and-unmyelinated-nerve-fibers-reinnervate-tissue-engineered-dermo-epidermal-human-skin-analogs-in-an-in-vivo-model
#11
T Biedermann, A S Klar, S Böttcher-Haberzeth, E Reichmann, M Meuli
PURPOSE: The clinical application of autologous tissue-engineered skin analogs is an important strategy to cover large skin defects. Investigating biological dynamics, such as reinnervation after transplantation, is essential to improve the quality of such skin analogs. Previously, we have examined that our skin substitutes are reinnervated by host peripheral nerve fibers as early as 8 weeks after transplantation. Here, we wanted to investigate the presence and possible differences regarding myelinated and unmyelinated host nerve fibers 15 weeks after the transplantation of light and dark human tissue-engineered skin analogs...
September 20, 2016: Pediatric Surgery International
https://www.readbyqxmd.com/read/27558287/a-micromechanical-comparison-of-human-and-porcine-skin-before-and-after-preservation-by-freezing-for-medical-device-development
#12
S A Ranamukhaarachchi, S Lehnert, S L Ranamukhaarachchi, L Sprenger, T Schneider, I Mansoor, K Rai, U O Häfeli, B Stoeber
Collecting human skin samples for medical research, including developing microneedle-based medical devices, is challenging and time-consuming. Researchers rely on human skin substitutes and skin preservation techniques, such as freezing, to overcome the lack of skin availability. Porcine skin is considered the best substitute to human skin, but their mechanical resemblance has not been fully validated. We provide a direct mechanical comparison between human and porcine skin samples using a conventional mechano-analytical technique (microindentation) and a medical application (microneedle insertion), at 35% and 100% relative humidity...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27547189/engineered-biopolymeric-scaffolds-for-chronic-wound-healing
#13
REVIEW
Laura E Dickinson, Sharon Gerecht
Skin regeneration requires the coordinated integration of concomitant biological and molecular events in the extracellular wound environment during overlapping phases of inflammation, proliferation, and matrix remodeling. This process is highly efficient during normal wound healing. However, chronic wounds fail to progress through the ordered and reparative wound healing process and are unable to heal, requiring long-term treatment at high costs. There are many advanced skin substitutes, which mostly comprise bioactive dressings containing mammalian derived matrix components, and/or human cells, in clinical use...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27529988/from-single-nucleotide-substitutions-up-to-chromosomal-deletions-genetic-pause-of-leucism-associated-disorders-in-animals
#14
REVIEW
Katharina Fleck, Georg Erhardt, Gesine Lühken
Leucism is characterized by a complete or partial white skin and hair in combination with pigmented irides, which can be vivid blue or heterochromatic. This is due to a complete or partial lack of melanocytes. The underlying pathogenesis is a disturbed emigration or differentiation of neural crest-derived cells. Therefore, leucistic phenotypes can be associated with defects, which mainly impair sensory organs and nerves. In humans, a well-known example is the Waardenburg syndrome. Leucism-associated disorders were also described in mouse, rat, hamster, rabbit, mink, cat, dog, pig, sheep, llama, alpaca, cattle and horse...
July 2016: Berliner und Münchener Tierärztliche Wochenschrift
https://www.readbyqxmd.com/read/27513102/tissue-engineered-human-psoriatic-skin-supplemented-with-cytokines-as-an-in%C3%A2-vitro-model-to-study-plaque-psoriasis
#15
Claudia Pouliot-Bérubé, Karine Zaniolo, Sylvain L Guérin, Roxane Pouliot
AIM: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. MATERIALS & METHODS: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-α, IL-1α, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. RESULTS: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU)...
September 2016: Regenerative Medicine
https://www.readbyqxmd.com/read/27494318/echoic-sensory-substitution-information-in-a-single-obstacle-circumvention-task
#16
Andrew J Kolarik, Amy C Scarfe, Brian C J Moore, Shahina Pardhan
Accurate motor control is required when walking around obstacles in order to avoid collisions. When vision is unavailable, sensory substitution can be used to improve locomotion through the environment. Tactile sensory substitution devices (SSDs) are electronic travel aids, some of which indicate the distance of an obstacle using the rate of vibration of a transducer on the skin. We investigated how accurately such an SSD guided navigation in an obstacle circumvention task. Using an SSD, 12 blindfolded participants navigated around a single flat 0...
2016: PloS One
https://www.readbyqxmd.com/read/27458264/hair-follicle-and-sebaceous-gland-de-novo-regeneration-with-cultured-epidermal-stem-cells-and-skin-derived-precursors
#17
Xiaoxiao Wang, Xusheng Wang, Jianjun Liu, Ting Cai, Ling Guo, Shujuan Wang, Jinmei Wang, Yanpei Cao, Jianfeng Ge, Yuyang Jiang, Edward E Tredget, Mengjun Cao, Yaojiong Wu
: : Stem cell-based organ regeneration is purported to enable the replacement of impaired organs in the foreseeable future. Here, we demonstrated that a combination of cultured epidermal stem cells (Epi-SCs) derived from the epidermis and skin-derived precursors (SKPs) was capable of reconstituting functional hair follicles and sebaceous glands (SG). When Epi-SCs and SKPs were mixed in a hydrogel and implanted into an excisional wound in nude mice, the Epi-SCs formed de novo epidermis along with hair follicles, and SKPs contributed to dermal papilla in the neogenic hair follicles...
July 25, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27421141/in-silico-predicted-structural-and-functional-insights-of-all-missense-mutations-on-2b-domain-of-k1-k10-causing-genodermatoses
#18
Santasree Banerjee, Qian Wu, Yuyi Ying, Yanni Li, Matsuyuki Shirota, Dante Neculai, Chen Li
The K1 and K10 associated genodermatoses are characterized by clinical symptoms of mild to severe redness, blistering and hypertrophy of the skin. In this paper, we set out to computationally investigate the structural and functional effects of missense mutations on the 2B domain of K1/K10 heterodimer and its consequences in disease phenotype. We modeled the structure of the K1/K10 heterodimer based on crystal structures for the human homolog K5/K14 heterodimer, and identified that the missense mutations exert their effects on stability and assembly competence of the heterodimer by altering physico-chemical properties, interatomic interactions, and inter-residue atomic contacts...
July 13, 2016: Oncotarget
https://www.readbyqxmd.com/read/27371895/polymeric-membranes-modulate-human-keratinocyte-differentiation-in-specific-epidermal-layers
#19
Simona Salerno, Sabrina Morelli, Francesca Giordano, Amalia Gordano, Loredana De Bartolo
In vitro models of human bioengineered skin substitutes are an alternative to animal experimentation for testing the effects and toxicity of drugs, cosmetics and pollutants. For the first time specific and distinct human epidermal strata were engineered by using membranes and keratinocytes. To this purpose, biodegradable membranes of chitosan (CHT), polycaprolactone (PCL) and a polymeric blend of CHT-PCL were prepared by phase-inversion technique and characterized in order to evaluate their morphological, physico-chemical and mechanical properties...
October 1, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27323213/toxicity-of-nanoparticle-surface-coating-agents-structure-cytotoxicity-relationship
#20
Ying Zhang, Xiaoping Li, Hongtao Yu
Surface coating agents for metal nanoparticles, cationic alkyl ammonium bromides and anionic alkyl sulfates were tested against human skin keratinocytes (HaCaT) and blood T lymphocytes (TIB-152). The surfactants of short chain (C8) are not cytotoxic, but as their chain length increases, their cytotoxicity increases and levels off at C12 for cationic surfactants against both cell lines and for anionic surfactants against the TIB-152, but C14 for anionic surfactants against HaCaT. The cationic surfactants are more toxic than the anionic surfactants for HaCaT; while with similar cytotoxicity for TIB-152 cells...
June 20, 2016: Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews
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