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Human skin substitute

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https://www.readbyqxmd.com/read/28792664/collagen-coated-nano-electrospun-pcl-seeded-with-human-endometrial-stem-cells-for-skin-tissue-engineering-applications
#1
Shiva Sharif, Jafar Ai, Mahmoud Azami, Javad Verdi, Mohammad Ali Atlasi, Sadegh Shirian, Ali Samadikuchaksaraei
Human endometrial stem cells (hEnSCs) are known as an attractive source of stem cells for regenerative medicine. hEnSCs are easily isolated and are capable of repairing uterine through their strong ability of creating new capillaries. In this study, a three-dimensional (3D) nanofibrous polycaprolactone (PCL)/collagen scaffold was fabricated and characterized in order to be applied as a new approach for skin reconstruction. Furthermore, the behavior of hEnSCs on this scaffold was investigated. First, a PCL 3D scaffold was constructed using electrospinning technique...
August 9, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28774630/the-chlorination-transformation-characteristics-of-benzophenone-4-in-the-presence-of-iodide-ions
#2
Fan Yang, Dongbin Wei, Ming Xiao, Xuefeng Sun, Qiaorong Guo, Yi Liu, Yuguo Du
Benzophenone-type UV filters are a group of compounds widely used to protect human skin from damage of UV irradiation. Benzophenone-4 (BP-4) was targeted to explore its transformation behaviors during chlorination disinfection treatment in the presence of iodide ions. With the help of ultra performance liquid phase chromatograph and high-resolution quadrupole time-of-flight mass spectrometer, totally fifteen halogenated products were identified, and five out of them were iodinated products. The transformation mechanisms of BP-4 involved electrophilic substitution generating mono- or di-halogenated products, which would be oxidized into esters and further hydrolyzed into phenolic derivatives...
August 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28774590/human-adipose-mesenchymal-cells-inhibit-melanocyte-differentiation-and-the-pigmentation-of-human-skin-via-increased-expression-of-tgf-%C3%AE-1
#3
Agnes S Klar, Thomas Biedermann, Katarzyna Michalak, Teresa Michalczyk, Claudia Meuli-Simmen, Arnaud Scherberich, Martin Meuli, Ernst Reichmann
There is accumulating evidence that interactions between epidermal melanocytes and stromal cells play an important role in the regulation of skin pigmentation. In this study we established a pigmented dermo-epidermal skin model (melDESS) of human origin to investigate the effects of distinct stromal cells on melanogenesis. melDESS is a complex, clinically relevant skin equivalent composed of an epidermis containing both melanocytes and keratinocytes. Its dermal compartment consisted either of adipose tissue-derived stromal cells (ASC), dermal fibroblasts, or a mixture of both cell types...
July 31, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28736162/-skin-engineering-for-severe-burns
#4
J-J Lataillade, B Magne, E Bey, T Leclerc, M Trouillas
Severe burned patients need definitive and efficient wound coverage. Outcome of massive burns has been improved by using cultured epithelial autografts (CEA). Despite fragility, percentages of success take, cost of treatment and long-term tendency to contracture, this surgical technique has been developed in few burn centres. First improvements were to combine CEA and dermis-like substitute. Cultured skin substitutes provide earlier skin closure and satisfying functional result. These methods have been used successfully in massive burns...
July 20, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/28715139/uvb-exposure-of-a-humanized-skin-model-reveals-unexpected-dynamic-of-keratinocyte-proliferation-and-wnt-inhibitor-balancing
#5
Teresa Michalczyk, Thomas Biedermann, Sophie Böttcher-Haberzeth, Agnes S Klar, Martin Meuli, Ernst Reichmann
We developed human dermo-epidermal skin substitutes which are presently applied in phase I and II clinical trials. Here we used these very same skin equivalents containing melanocytes, named MelSkin, as an experimental skin model. We investigated the effects of UVB irradiation on the skin grafts transplanted on immune-compromised rats. The irradiation induces a strong wound healing response going along with massive proliferation of basal keratinocytes, basically quiescent under non-irradiated, homeostatic conditions...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28711498/promotion-of-hair-growth-by-newly-synthesized-ceramide-mimetic-compound
#6
Bu-Mahn Park, Soon-Sun Bak, Kyung-Oh Shin, Minhee Kim, Daehwan Kim, Sang-Hun Jung, Sekyoo Jeong, Young Kwan Sung, Hyun Jung Kim
Based on the crucial roles of ceramides in skin barrier function, use of ceramides or their structural mimetic compounds, pseudoceramides, as cosmetic ingredients are getting more popular. While currently used pseudoceramides are intended to substitute the structural roles of ceramides in stratum corneum, development of bioactive pseudoceramides has been repeatedly reported. In this study, based on the potential involvement of sphingolipids in hair cycle regulation, we investigated the effects of newly synthesized pseudoceramide, bis-oleamido isopropyl alcohol (BOI), on hair growth using cultured human hair follicles and animal models...
July 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28702969/bi-layered-collagen-nano-structured-membrane-prototype-collagen-matrix-10826%C3%A2-for-soft-tissue-regeneration-in-rabbits-an-in-vivo-ultra-structural-study-of-the-early-healing-phase
#7
D De Santis, G B Menchini Fabris, J Lotti, C Palumbo, M Ferretti, R Castellani, T Lotti, G Zanotti, F Gelpi, C Covani, P F Nocini
Collagen Matrix (CM) 10826 is a nanostructured bi-layered collagen membrane obtained from type I and III porcine collagen, which in vitro has shown to have the potential to be a substitute and/or stimulant for soft oral tissue regeneration. The objective of this study was to evaluate the in vivo potential and safety of this membrane for soft tissue regeneration in the early stage of wound healing. Two soft tissue wounds (test and control) were created on the back skin of 5 rabbits (female New Zealand White Rabbits specific pathogen free)...
July 13, 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28684336/coculture-of-mesenchymal-stem-cells-and-endothelial-cells-enhances-host-tissue-integration-and-epidermis-maturation-through-akt-activation-in-gelatin-methacryloyl-hydrogel-based-skin-model
#8
Xiaofei Zhang, Jun Li, Pengxiang Ye, Guifang Gao, Karen Hubbell, Xiaofeng Cui
A major challenge for clinical use of skin substitutes is insufficient host tissue integration leading to loosening and partial necrosis of the implant. In this present study, a three-dimensional (3D) coculture system constructed using human umbilical cord mesenchymal stem cells (uc-MSCs) and umbilical vein endothelial cells (HUVECs) encapsulated in gelatin methacryloyl (GelMA) hydrogels was evaluated to determine the outcomes of cell-cell interactions in vitro and in vivo. The results revealed that GelMA hydrogels displayed minor cytotoxicity on both cell types...
September 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28657190/repeated-subcutaneous-administrations-of-krokodil-causes-skin-necrosis-and-internal-organs-toxicity-in-wistar-rats-putative-human-implications
#9
Emanuele Amorim Alves, Pedro Brandão, João Filipe Neves, Sara Manuela Cravo, José Xavier Soares, Jean-Paul C Grund, José Alberto Duarte, Carlos M M Afonso, Annibal Duarte Pereira Netto, Félix Carvalho, Ricardo Jorge Dinis-Oliveira
OBJECTIVE: "Krokodil" is the street name for an impure homemade drug mixture used as a cheap substitute for heroin, containing desomorphine as the main opioid. Abscesses, gangrene, thrombophlebitis, limb ulceration and amputations, jaw osteonecrosis, skin discoloration, ulcers, skin infections, and bleeding are some of the typical reported signs in humans. This study aimed to understand the toxicity of krokodil using Wistar male rats as experimental model. METHODS: Animals were divided into seven groups and exposed subcutaneously to NaCl 0...
May 2017: Human Psychopharmacology
https://www.readbyqxmd.com/read/28640957/restoration-of-cutaneous-pigmentation-by-transplantation-to-mice-of-isogeneic-human-melanocytes-in-dermal-epidermal-engineered-skin-substitutes
#10
Steven T Boyce, Christopher M Lloyd, Mark C Kleiner, Viki B Swope, Zalfa Abdel-Malek, Dorothy M Supp
Autologous engineered skin substitutes (ESS) containing melanocytes (hM) may restore pigmentation, and photoprotection after grafting to full-thickness skin wounds. In the current study, normal hM were isolated from discard skin, propagated with or without tyrosinase inhibitors, cryopreserved, recovered into culture, and added to ESS (ESS-P) before transplantation. ESS-P were incubated in either UCMC160/161 or UCDM1 medium, scored for hM densities, and grafted to mice. The results showed that: sufficient hM can be propagated to expand donor tissue by 100-fold; incubation of hM in tyrosinase inhibitors reduced pigment levels but did not change hM recovery after cryopreservation; hM densities in ESS-P were greater after incubation in UCDM1 than UCMC160 medium; hM were localized to the dermal-epidermal junction of ESS-P; and UCDM1 medium promoted earlier pigment distribution and density...
June 22, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/28615133/platelet-rich-plasma-with-keratinocytes-and-fibroblasts-enhance-healing-of-full-thickness-wounds
#11
Jia Xian Law, Shiplu Roy Chowdhury, Aminuddin Bin Saim, Ruszymah Bt Hj Idrus
Advances in tissue engineering led to the development of various tissue-engineered skin substitutes (TESS) for the treatment of skin injuries. The majority of the autologous TESS required lengthy and costly cell expansion process to fabricate. In this study, we determine the possibility of using a low density of human skin cells suspended in platelet-rich plasma (PRP)-enriched medium to promote the healing of full-thickness skin wounds. To achieve this, full-thickness wounds of size 1.767 cm(2) were created at the dorsum part of nude mice and treated with keratinocytes (2 × 10(4) cells/cm(2)) and fibroblasts (3 × 10(4) cells/cm(2)) suspended in 10% PRP-enriched medium...
June 7, 2017: Journal of Tissue Viability
https://www.readbyqxmd.com/read/28613256/strawberry-based-cosmetic-formulations-protect-human-dermal-fibroblasts-against-uva-induced-damage
#12
Massimiliano Gasparrini, Tamara Y Forbes-Hernandez, Sadia Afrin, Patricia Reboredo-Rodriguez, Danila Cianciosi, Bruno Mezzetti, Josè L Quiles, Stefano Bompadre, Maurizio Battino, Francesca Giampieri
Extreme exposure of skin to Ultraviolet A (UVA)-radiation may induce a dysregulated production of reactive oxygen species (ROS) which can interact with cellular biomolecules leading to oxidative stress, inflammation, DNA damage, and alteration of cellular molecular pathways, responsible for skin photoaging, hyperplasia, erythema, and cancer. For these reasons, the use of dietary natural bioactive compounds with remarkable antioxidant activity could be a strategic tool to counteract these UVA-radiation-caused deleterious effects...
June 14, 2017: Nutrients
https://www.readbyqxmd.com/read/28585891/in%C3%A2-vitro-psoriasis-models-with-focus-on-reconstructed-skin-models-as-promising-tools-in-psoriasis-research
#13
Eline Desmet, Anesh Ramadhas, Jo Lambert, Mireille Van Gele
Psoriasis is a complex chronic immune-mediated inflammatory cutaneous disease associated with the development of inflammatory plaques on the skin. Studies proved that the disease results from a deregulated interplay between skin keratinocytes, immune cells and the environment leading to a persisting inflammatory process modulated by pro-inflammatory cytokines and activation of T cells. However, a major hindrance to study the pathogenesis of psoriasis more in depth and subsequent development of novel therapies is the lack of suitable pre-clinical models mimicking the complex phenotype of this skin disorder...
June 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28576031/fabrication-of-a-three-dimensional-spongy-scaffold-using-human-wharton-s-jelly-derived-extra-cellular-matrix-for-wound-healing
#14
Bahareh Beiki, Bahman Zeynali, Ehsan Seyedjafari
The Wharton's jelly (WJ) contains significant amounts of extracellular matrix (ECM) components and rich source of endogenous growth factors. In this study, we designed a new biomimetic spongy scaffold from decellularized WJ-derived ECM and used it as a skin substitute. Histological analysis and biochemical assays showed that bio-active molecules preserved in the fabricated scaffolds and that the scaffolds have highly interconnected porous structure. Cytotoxicity and mechanical evaluation of the scaffold indicated that it is non-toxic and has appropriate mechanical properties...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28522073/cell-based-skin-substitutes-accelerate-regeneration-of-extensive-burn-wounds-in-rats
#15
Sadrollah Motamed, Ehsan Taghiabadi, Hojjat Molaei, Niloofar Sodeifi, Seyed Esmaeil Hassanpour, Saeed Shafieyan, Enzollah Azargashb, Fatemeh Farajzadeh-Vajari, Nasser Aghdami, Amir Bajouri
BACKGROUND: This study investigated the effects of amniotic membrane combined with adipose-derived stem cells or fetal fibroblasts on regenerating extensive burns in rats. METHODS: Third degree burns of 1100-1800 mm(2) were induced on 32 Sprague-Dawley rats. Burned sites were excised and randomly covered with Vaseline gauze (control), human amniotic membrane (HAM), human fetal fibroblasts seeded on HAM (HAM-FF), or human adipose-derived stem cells seeded on HAM (HAM-ASC), and followed by wound closure and histological assessments...
April 27, 2017: American Journal of Surgery
https://www.readbyqxmd.com/read/28506627/rxrb-is-a-mhc-encoded-susceptibility-gene-associated-with-anti-topoisomerase-i-antibody-positive-systemic-sclerosis
#16
Akira Oka, Yoshihide Asano, Minoru Hasegawa, Manabu Fujimoto, Osamu Ishikawa, Masataka Kuwana, Yasushi Kawaguchi, Toshiyuki Yamamoto, Hiroki Takahashi, Daisuke Goto, Hirahito Endo, Masatoshi Jinnin, Shuhei Mano, Kazuyoshi Hosomichi, Tomotaka Mabuchi, Mahoko Takahashi Ueda, So Nakagawa, Stephan Beck, Seiamak Bahram, Kazuhiko Takehara, Shinichi Sato, Hironobu Ihn
Systemic sclerosis (SSc) is a systemic autoimmune and connective tissue disorder associated with the human leukocyte antigen (HLA) locus. However, the functional relationship between HLA gene(s) and disease development remains unknown. To elucidate major histocompatibility complex (MHC)-linked SSc genetics, we performed genotyping of MHC-borne microsatellites and HLA-DPB1 alleles using DNA obtained from 318 anti-topoisomerase I antibody-positive patients and 561 healthy controls, all of Japanese descent. Those results revealed 2 MHC haplotypes associated with SSc...
May 12, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28457550/effects-of-rare-sugar-d-allulose-on-acid-production-and-probiotic-activities-of-dairy-lactic-acid-bacteria
#17
H Kimoto-Nira, N Moriya, S Hayakawa, K Kuramasu, H Ohmori, S Yamasaki, M Ogawa
It has recently been reported that the rare sugar d-allulose has beneficial effects, including the suppression of postprandial blood glucose elevation in humans, and can be substituted for sucrose as a low-calorie food ingredient. To examine the applications of d-allulose in the dairy industry, we investigated the effects of d-allulose on the acid production of 8 strains of yogurt starter (Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus) and 4 strains of lactococci, including potential probiotic candidates derived from dairy products...
July 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28412341/chitosan-a-potential-biopolymer-for-wound-management
#18
REVIEW
Ijaz Bano, Muhammad Arshad, Tariq Yasin, Muhammad Afzal Ghauri, Muhammad Younus
It has been seen that slow healing and non-healing wounds conditions are treatable but still challenging to humans. Wound dressing usually seeks for biocompatible and biodegradable recipe. Natural polysaccharides like chitosan have been examined for its antimicrobial and healing properties on the basis of its variation in molecular weight and degree of deacetylation. Chitosan adopts some vital characteristics for treatment of various kinds of wounds which include its bonding nature, antifungal, bactericidal and permeability to oxygen...
September 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28403359/oral-mucosa-an-alternative-epidermic-cell-source-to-develop-autologous-dermal-epidermal-substitutes-from-diabetic-subjects
#19
Daniela Guzmán-Uribe, Keila Neri Alvarado-Estrada, Mauricio Pierdant-Pérez, Bertha Torres-Álvarez, Jesus Martin Sánchez-Aguilar, Raúl Rosales-Ibáñez
Objective: The aim of this study was to obtain autologous dermal-epidermal skin substitutes from oral mucosa from diabetic subjects as a first step towards a possible clinical application for cases of diabetic foot. Material and Methods: Oral mucosa was obtained from diabetic and healthy subjects (n=20 per group). Epidermal cells were isolated and cultured using autologous fibrin to develop dermal-epidermal in vitro substitutes by the air-liquid technique with autologous human serum as a supplement media...
March 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28394544/fibrin-gel-as-a-scaffold-for-skin-substitute-production-and-clinical-experience
#20
(no author information available yet)
The purpose of this study was to create a fibrin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaff olds were prepared from commercial fibrin glue kits. Human fibroblasts were cultured in fibrin gel. Human keratinocytes were seeded on the top of the gel. Viability of cells was determined fluorimetrically. Scanning electron microscope and immunocytochemistry analysis of cultured cells were performed...
June 2016: Acta Clinica Croatica
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