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Human skin substitute

Juliane Ertl, Melanie Pichlsberger, Alexandru-Cristian Tuca, Paul Wurzer, Jakob Fuchs, Stefan H Geyer, Barbara Maurer-Gesek, Wolfgang J Weninger, Dagmar Pfeiffer, Vladimir Bubalo, Daryousch Parvizi, Lars-Peter Kamolz, Ingrid Lang
OBJECTIVE: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. METHODS: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm® as carrier and evaluated wound healing parameters...
May 2018: Placenta
Sarah E Hancock, Ramesh Ailuri, David L Marshall, Simon H J Brown, Jennifer T Saville, Venkateswara R Narreddula, Nathan R Boase, Berwyck L J Poad, Adam J Trevitt, Mark D P Willcox, Michael J Kelso, Todd W Mitchell, Stephen J Blanksby
The (O-acyl)-ω-hydroxy fatty acids (OAHFA) are an unusual lipid subclass present in the skin, vernix caseosa and meibomian gland secretions. While they are structurally related to the general class of fatty acid esters of hydroxy fatty acids (FAHFA), the ultra-long chain (30-34 carbons) and putative ω-substitution of the backbone hydroxy fatty acid suggest OAHFA have unique biochemistry. Complete structural elucidation of OAHFA has been challenging due to their low abundance within complex lipid matrices...
June 15, 2018: Journal of Lipid Research
Zihui Fang, Jiujiu Huang, Jie Chen, Shaopeng Xu, Zhaojian Xiang, Mei Hong
Organic anion transporting polypeptides (OATPs, gene symbol SLCO) are important membrane transporter proteins that mediate the uptake of wide ranges of endogenous and exogenous compounds. OATP2B1 has been found in multiple organs and tissues including the liver, small intestine, kidney, brain, placenta, heart, skin as well as skeletal muscle and is proposed to be involved in the uptake of orally administered drugs. Quite a few reports have demonstrated that transmembrane domains (TMs) are crucial for proper functions of OATP family members...
June 5, 2018: Molecular Pharmacology
Michael A Towler, Elaine W Rush, Melissa K Richardson, Calvin L Williams
Chronic venous leg ulcers are responsible for significant morbidity and health care costs worldwide. This pilot study evaluated the effectiveness 2 biologically active grafts, TheraSkin and Apligraf, in conjunction with compression therapy. The study, not industry-sponsored, was designed and conducted as a prospective, head-to-head, single-site, randomized clinical trial to assess differences in healing rates, adverse outcomes, and treatment costs. The healing rates were different but not statistically significant, there were no adverse outcomes, and TheraSkin averaged $2495...
July 2018: Clinics in Podiatric Medicine and Surgery
Wen-Shyan Huang, Yi-Wen Wang, Kun-Che Hung, Pai-Shan Hsieh, Keng-Yen Fu, Lien-Guo Dai, Nien-Hsien Liou, Kuo-Hsing Ma, Jiang-Chuan Liu, Niann-Tzyy Dai
Background: To treat skin color disorders, such as vitiligo or burns, melanocytes are transplanted for tissue regeneration. However, melanocyte distribution in the human body varies with age and location, making it difficult to select the optimal donor skin to achieve a desired color match. Determining the correlations with the desired skin color measurement based on CIELAB color, epidermal melanocyte numbers, and melanin content of individual melanocytes is critical for clinical application...
2018: PeerJ
Gaëtan Le-Bel, Sergio Cortez Ghio, Danielle Larouche, Lucie Germain, Sylvain L Guérin
Electrophoretic mobility shift assays and Western blots are simple, efficient, and rapid methods to study DNA-protein interactions and protein expression, respectively. Primary cultures and subcultures of epithelial cells are widely used for the production of tissue-engineered substitutes and are gaining popularity as a model for gene expression studies. The preservation of stem cells through the culture process is essential to produce high quality substitutes. However, the increase in the number of cell passages is associated with a decrease in their ability to proliferate until senescence is reached...
May 27, 2018: Methods in Molecular Biology
Pengju Zhao, Ying Yu, Wen Feng, Heng Du, Jian Yu, Huimin Kang, Xianrui Zheng, Zhiquan Wang, George E Liu, Catherine W Ernst, Xueqin Ran, Jiafu Wang, Jian-Feng Liu
Background: Meishan is a pig breed indigenous to China and famous for its high fecundity. The traits of Meishan are strongly associated with its distinct evolutionary history and domestication. However, the genomic evidence linking the domestication of Meishan pigs with the unique features are still poorly understood. The goal of this study is to investigate the genomic signatures and evolutionary evidence related to phenotypic traits of Meishan by large-scale sequencing. Results: We found the unique domestication of Meishan pigs happened at the Taihu Basin area between the Majiabang and the Liangzhu culture, during which 300 protein-coding genes have undergone positive selection...
May 22, 2018: GigaScience
Robin Augustine
Significant progress has been made over the past few decades in the development of in vitro-engineered substitutes that mimic human skin, either as grafts for the replacement of lost skin, or for the establishment of in vitro human skin models. Tissue engineering has been developing as a novel strategy by employing the recent advances in various fields such as polymer engineering, bioengineering, stem cell research and nanomedicine. Recently, an advancement of 3D printing technology referred as bioprinting was exploited to make cell loaded scaffolds to produce constructs which are more matching with the native tissue...
May 12, 2018: Progress in Biomaterials
J Zundel, S A Ansari, H M Trivedi, J G Masters, S Mascaro
BACKGROUND: The purpose of this research is to characterize the effects of mouthwash solutions on oral friction and moisture using a quantitative in vitro approach. MATERIALS AND METHODS: The frictional coefficient of in vitro porcine tongue samples was measured using a magnetic levitation haptic device equipped with a custom tactor designed to mimic human skin. A commercially available moisture meter was used to measure moisture content of the samples. Tongue samples were first tested before treatment, then after application of saliva (either human or artificial), and again after application of 1 of 11 different mouthwash solutions...
May 7, 2018: Skin Research and Technology
Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, Amir Bajouri
Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11-14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells...
May 8, 2018: Methods in Molecular Biology
Bryan D Moyer, Justin K Murray, Joseph Ligutti, Kristin Andrews, Philippe Favreau, John B Jordan, Josie H Lee, Dong Liu, Jason Long, Kelvin Sham, Licheng Shi, Reto Stöcklin, Bin Wu, Ruoyuan Yin, Violeta Yu, Anruo Zou, Kaustav Biswas, Les P Miranda
Identification of voltage-gated sodium channel NaV1.7 inhibitors for chronic pain therapeutic development is an area of vigorous pursuit. In an effort to identify more potent leads compared to our previously reported GpTx-1 peptide series, electrophysiology screening of fractionated tarantula venom discovered the NaV1.7 inhibitory peptide JzTx-V from the Chinese earth tiger tarantula Chilobrachys jingzhao. The parent peptide displayed nominal selectivity over the skeletal muscle NaV1.4 channel. Attribute-based positional scan analoging identified a key Ile28Glu mutation that improved NaV1...
2018: PloS One
Qian Xu, Linru Guo, Sigen A, Yongsheng Gao, Dezhong Zhou, Udo Greiser, Jack Creagh-Flynn, Hong Zhang, Yixiao Dong, Lara Cutlar, Fagang Wang, Wenguang Liu, Wei Wang, Wenxin Wang
Adjusting biomaterial degradation profiles to match tissue regeneration is a challenging issue. Herein, biodegradable hyperbranched poly(β-amino ester)s (HP-PBAEs) were designed and synthesized via "A2 + B4" Michael addition polymerization, and displayed fast gelation with thiolated hyaluronic acid (HA-SH) via a "click" thiol-ene reaction. HP-PBAE/HA-SH hydrogels showed tunable degradation profiles both in vitro and in vivo using diamines with different alkyl chain lengths and poly(ethylene glycol) diacrylates with varied PEG spacers...
February 28, 2018: Chemical Science
Arun Prabhu Rameshbabu, Kamakshi Bankoti, Sayanti Datta, Elavarasan Subramani, Anupam Apoorva, Paulomi Ghosh, Priti Prasanna Maity, Padmavati Manchikanti, Koel Chaudhury, Santanu Dhara
Regeneration of full-thickness wound without scar formation is a multifaceted process which depends on in situ dynamic interactions between the tissue-engineered skin substitutes and newly formed reparative tissue. However, the majority of the tissue-engineered skin substitutes used so far in full-thickness wound healing cannot mimic the natural extracellular matrix (ECM) complexity and thus are incapable of providing a suitable niche for endogenous tissue repair. Herein, we demonstrated a simple approach to fabricate porous Hybrid Extracellular Matrix Sponges (HEMS) using Placental Extracellular Matrix (pECM) and Silk Fibroin (SF) for full-thickness wound healing...
May 2, 2018: ACS Applied Materials & Interfaces
Ewa Krzywinska, Marek Dominick Zorawski, Agnieszka Taracha, Grzegorz Kotarba, Agnieszka Kikulska, Michal Mlacki, Katarzyna Kwiatkowska, Tomasz Wilanowski
The mammalian Grainyhead-like 3 (GRHL3) transcription factor is essential for epithelial development and plays a protective role against squamous cell carcinoma of the skin and of the oral cavity. A single nucleotide polymorphism (SNP) in GRHL3, rs141193530 (p.P455A), is associated with non-melanoma skin cancer in human patients. Moreover, it is known that this SNP, as well as another variant, rs41268753 (p.T454M), are associated with nonsyndromic cleft palate and that rs41268753 negatively affects GRHL3 transcriptional activity...
July 2018: Biochimica et Biophysica Acta
Lia M G Neves, Nivaldo A Parizotto, Marcia R Cominetti, Ardeshir Bayat
The use of dermal substitutes to treat skin defects such as ulcers has shown promising results, suggesting a potential role for skin substitutes for treating acute and chronic wounds. One of the main drawbacks with the use of dermal substitutes is the length of time from engraftment to graft take, plus the risk of contamination and failure due to this prolonged integration. Therefore, the use of adjuvant energy-based therapeutic modalities to augment and accelerate the rate of biointegration by dermal substitute engraftments is a desirable outcome...
April 24, 2018: Journal of Biophotonics
Carolina Torres, Melina Elizabeth Barrios, Robertina Viviana Cammarata, Matías Victoria, Xavier Fernandez-Cassi, Silvia Bofill-Mas, Rodney Colina, María Dolores Blanco Fernández, Viviana Andrea Mbayed
New human polyomaviruses have been described in the last years, including the Merkel-cell polyomavirus (MCPyV; Human polyomavirus 5) and the Human polyomavirus 6 (HPyV6). Although their infection is usually asymptomatic, in immunocompromised host can cause life-threatening pathologies, such as the Merkel cell carcinoma, an aggressive skin neoplasia associated to the MCPyV. Despite being prevalent viruses in population, epidemiological data from South America are scarce, as well as the characterization of the viral types circulating and their origin...
April 20, 2018: Molecular Phylogenetics and Evolution
Saghar Mowlazadeh Haghighi, Yang Zhou, Jixun Dai, Jonathon R Sawyer, Victor J Hruby, Minying Cai
Melanoma skin cancer is the fastest growing cancer in the US [1]. A great need exists for improved formulations and mechanisms to prevent and protect human skin from cancers and other skin damage caused by sunlight exposure. Current efforts to prevent UV damage to human skin, which in many cases leads to melanoma and other skin cancers. The primordial melanocortin-1 receptor (MC1R) is involved in regulating skin pigmentation and hair color, which is a natural prevention from UV damage. The endogenous melanocortin agonists induce pigmentation and share a core pharmacophore sequence "His-Phe-Arg-Trp", and it was found that substitution of the Phe by D-Phe results in increasing melanocortin receptor potency...
May 10, 2018: European Journal of Medicinal Chemistry
Lara Cristóbal, Miguel A Ortega, Ángel Asúnsolo, Beatriz Romero, Melchor Álvarez-Mon, Julia Buján, Andrés A Maldonado, Natalio García-Honduvilla
Despite advances in regenerative medicine and tissue engineering, human skin substitutes remain a clear goal to achieve. Autografts remain the principal clinical option. The long-term changes in dermis, as well as its response after injuries, are not well known. Research in this field has been hindered by a lack of experimental animal models. This study analyzes the architectural dermal scaffold (collagen and elastin fibers plus fibrillin-microfibrils) changes in a model of human skin pressure ulcers in mice...
April 17, 2018: Histology and Histopathology
Cornelius Domhan, Philipp Uhl, Anna Meinhardt, Stefan Zimmermann, Christian Kleist, Thomas Lindner, Karin Leotta, Walter Mier, Michael Wink
As evolutionary optimized defense compounds, antimicrobial peptides (AMPs) represent a powerful tool against bacterial infections. Ranalexin, an AMP found in the skin of the American bullfrog (Rana catesbeiana), is primarily active against Gram-positive bacteria with a minimum inhibitory concentration (MIC) of 8 - 16 mg/L but shows weaker activity against Gram-negative bacteria (> 64 mg/L). By substitution of 6 N-terminal amino acids by decanoic (C10:0) to myristic (C14:0) acid, we were able to develop lipopeptide derivatives with enhanced antimicrobial activity...
April 9, 2018: International Journal of Antimicrobial Agents
Luyao Zhang, Xiaoling Chen, Yue Wu, Mei Zhou, Chengbang Ma, Xinping Xi, Tianbao Chen, Brian Walker, Chris Shaw, Lei Wang
The first amphibian skin secretion-derived Bowman-Birk type chymotrypsin inhibitor is described here from the Asian green frog, Hylarana erythraea, and was identified by use of molecular cloning and tandem mass spectrometric amino acid sequencing. It was named Hylarana erythraea chymotrypsin inhibitor (HECI) and in addition to inhibition of chymotrypsin (Ki = 3.92 ± 0.35 μM), the peptide also inhibited the 20 S proteasome (Ki = 8.55 ± 1.84  μM). Additionally, an analogue of HECI, named K9 -HECI, in which Phe9 was substituted by Lys9 at the P1 position, was functional as a trypsin inhibitor...
April 11, 2018: Scientific Reports
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