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https://www.readbyqxmd.com/read/29346445/transcriptome-analysis-of-skin-fibroblasts-with-dominant-negative-col3a1-mutations-provides-molecular-insights-into-the-etiopathology-of-vascular-ehlers-danlos-syndrome
#1
Nicola Chiarelli, Giulia Carini, Nicoletta Zoppi, Marco Ritelli, Marina Colombi
Vascular Ehlers-Danlos syndrome (vEDS) is a dominantly inherited connective tissue disorder caused by mutations in the COL3A1 gene that encodes type III collagen (COLLIII), which is the major expressed collagen in blood vessels and hollow organs. The majority of disease-causing variants in COL3A1 are glycine substitutions and in-frame splice mutations in the triple helix domain that through a dominant negative effect are associated with the severe clinical spectrum potentially lethal of vEDS, characterized by fragility of soft connective tissues with arterial and organ ruptures...
2018: PloS One
https://www.readbyqxmd.com/read/29342076/neuromorphic-vibrotactile-stimulation-of-fingertips-for-encoding-object-stiffness-in-telepresence-sensory-substitution-and-augmentation-applications
#2
Francesca Sorgini, Luca Massari, Jessica D'Abbraccio, Eduardo Palermo, Arianna Menciassi, Petar B Petrovic, Alberto Mazzoni, Maria Chiara Carrozza, Fiona N Newell, Calogero M Oddo
We present a tactile telepresence system for real-time transmission of information about object stiffness to the human fingertips. Experimental tests were performed across two laboratories (Italy and Ireland). In the Italian laboratory, a mechatronic sensing platform indented different rubber samples. Information about rubber stiffness was converted into on-off events using a neuronal spiking model and sent to a vibrotactile glove in the Irish laboratory. Participants discriminated the variation of the stiffness of stimuli according to a two-alternative forced choice protocol...
January 17, 2018: Sensors
https://www.readbyqxmd.com/read/29337163/utility-of-ultra-deep-sequencing-for-detection-of-varicella-zoster-virus-antiviral-resistance-mutations
#3
Mélanie Mercier-Darty, David Boutolleau, Raphaël Lepeule, Christophe Rodriguez, Sonia Burrel
We report the first application of ultra-deep sequencing (UDS) to varicella-zoster virus (VZV) genotypic antiviral testing in a case of acyclovir-resistant VZV infection initially detected by Sanger sequencing within a deeply immunocompromised heart transplant recipient. As added-value compared to Sanger analysis, UDS revealed complex dynamics of viral population under antiviral pressure. Varicella-zoster virus (VZV) is a ubiquitous human herpesvirus affecting populations worldwide. VZV is commonly acquired in youth whose primary infection usually manifests as benign varicella (chickenpox)...
January 11, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29336231/a-new-and-universal-classification-system-of-skin-substitutes-inspired-by-factorial-design
#4
Evan Davison-Kotler, Vaibhav Sharma, Norbert Kang, Elena García-Gareta
The complexity of the dermal layer of skin means that damage to this section can result in permanent impairment of function. Partial or total dermal loss is a feature of deep burns and chronic wounds such as pressure sores or diabetic ulcers. The issues posed by traditional skin grafts have led to substantial research being carried out in the fields of tissue engineering and biomaterials science to develop a vast array of alternative skin substitutes. Given the large number of different materials, manufacturing methods, and techniques for implementation described for artificial skin substitutes, many classification systems have been created to simplify their categorization...
January 16, 2018: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/29334134/a-comprehensive-next-generation-sequencing-assay-for-the-diagnosis-of-epidermolysis-bullosa
#5
Anne W Lucky, Neha Dagaonkar, Karen Lammers, Ammar Husami, Diane Kissell, Kejian Zhang
BACKGROUND: Historically, diagnosis of epidermolysis bullosa has required skin biopsies for electron microscopy, direct immunofluorescence to determine which gene(s) to choose for genetic testing, or both. METHODS: To avoid these invasive tests, we developed a high-throughput next-generation sequencing (NGS)-based diagnostic assay called EBSEQ that allows simultaneous detection of mutations in 21 genes with known roles in epidermolysis bullosa pathogenicity. Mutations are confirmed with traditional Sanger sequencing...
January 15, 2018: Pediatric Dermatology
https://www.readbyqxmd.com/read/29324789/an-l213a-variant-of-%C3%AE-glycosidase-from-sulfolobus-solfataricus-with-increased-%C3%AE-l-arabinofuranosidase-activity-converts-ginsenoside-rc-to-compound-k
#6
Ji-Hyeon Choi, Kyung-Chul Shin, Deok-Kun Oh
Compound K (C-K) is a crucial pharmaceutical and cosmetic component because of disease prevention and skin anti-aging effects. For industrial application of this active compound, the protopanaxadiol (PPD)-type ginsenosides should be transformed to C-K. β-Glycosidase from Sulfolobus solfataricus has been reported as an efficient C-K-producing enzyme, using glycosylated PPD-type ginsenosides as substrates. β-Glycosidase from S. solfataricus can hydrolyze β-d-glucopyranoside in ginsenosides Rc, C-Mc1, and C-Mc, but not α-l-arabinofuranoside in these ginsenosides...
2018: PloS One
https://www.readbyqxmd.com/read/29324430/a-prospective-multicenter-study-to-compare-a-disposable-high-fluid-capacity-underpad-to-nonpermeable-disposable-reusable-containment-products-on-incontinence-associated-dermatitis-rates-among-skilled-nursing-facility-residents
#7
Glenda Motta, Catherine T Milne
Due to the high prevalence of incontinence among skilled nursing facility (SNF) residents, incontinence-associated derma- titis (IAD) is a common occurrence. In addition, facility staff may mistakenly identify IAD as a pressure injury. A prospective, descriptive, multicenter study was conducted in 3 Connecticut facilities to evaluate the effect of substituting a disposable, high- uid capacity underpad for nonpermeable disposable and reusable containment products on the rate of IADs. Residents with and without IAD but with high IAD risk scores who were bed- or chairbound or ambulatory and used disposable nonpermeable briefs and underpads or reusable, laundered containment products when in bed longer than 2 hours were randomly enrolled and observed for a 4-week period...
December 2017: Ostomy/wound Management
https://www.readbyqxmd.com/read/29322228/-necrotizing-fasciitis-of-the-hand-and-forearm-acute-surgical-treatment-and-defect-reconstruction-with-matriderm%C3%A2-and-split-thickness-skin-graft
#8
B Niedermueller, G Singer, P Pickl, M Jesacher
This case report describes a 55-year-old male patient with type II necrotizing fasciitis (NF) of the hand and forearm. The rapid progression of the tissue infection could be successfully stopped with radical surgical débridement and antibiotic therapy. For the reconstruction of the extensive loss of soft tissue a combination of split-thickness skin graft (STSG) and the synthetic dermal substitute MatriDerm® was used. In cases of NF, MatriDerm® and STSG provide a rapidly available and simple alternative to other reconstruction techniques...
January 10, 2018: Der Unfallchirurg
https://www.readbyqxmd.com/read/29320588/a-guide-to-tissue-engineered-skin-substitutes
#9
Penelope J Kallis, Adam J Friedman, Hadar Lev-Tov
<p>Wounds that exhibit delayed healing have a tremendous impact on health care expenditures and place patients at serious risk for severe complications including death. The healing of a chronic wound requires the restoration of multiple factors that normally work in concert to repair the damaged skin barrier. Skin substitutes have shown great promise for use as adjunctive therapies for refractory wounds by providing cells, soluble mediators, and extracellular matrix materials needed to stimulate healing...
January 1, 2018: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/29316315/scarless-wound-healing-transitioning-from-fetal-research-to-regenerative-healing
#10
REVIEW
Alessandra L Moore, Clement D Marshall, Leandra A Barnes, Matthew P Murphy, Ryan C Ransom, Michael T Longaker
Since the discovery of scarless fetal skin wound healing, research in the field has expanded significantly with the hopes of advancing the finding to adult human patients. There are several differences between fetal and adult skin that have been exploited to facilitate scarless healing in adults including growth factors, cytokines, and extracellular matrix substitutes. However, no one therapy, pathway, or cell subtype is sufficient to support scarless wound healing in adult skin. More recently, products that contain or mimic fetal and adult uninjured dermis were introduced to the wound healing market with promising clinical outcomes...
January 9, 2018: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29314321/percutaneous-fetoscopic-closure-of-large-open-spina-bifida-using-a-bilaminar-skin-substitute
#11
Denise A Lapa Pedreira, Gregório L Acacio, Rodrigo T Gonçalves, Renato Augusto M Sá, Reynaldo A Brandt, Ramen Chmait, Eftichia Kontopoulos, Ruben A Quintero
OBJECTIVE: We have previously described our percutaneous fetoscopic technique for the treatment of open spina bifida (OSB). However, approximately 20-30% of OSB defects are too large to allow primary skin closure. We hereby describe a modification of our standard technique using a bilaminar skin substitute to allow closure of such large spinal defects. The aim of this study was to report our clinical experience with the use of a bilaminar skin substitute and a percutaneous fetoscopic technique for the prenatal closure of large spina bifida defects...
January 4, 2018: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29313702/a-comparison-of-scaffold-free-and-scaffold-based-reconstructed-human-skin-models-as-alternatives-to-animal-use
#12
Beste Kinikoglu
Tissue engineered full-thickness human skin substitutes have various applications in the clinic and in the laboratory, such as in the treatment of burns or deep skin defects, and as reconstructed human skin models in the safety testing of drugs and cosmetics and in the fundamental study of skin biology and pathology. So far, different approaches have been proposed for the generation of reconstructed skin, each with its own advantages and disadvantages. Here, the classic tissue engineering approach, based on cell-seeded polymeric scaffolds, is compared with the less-studied cell self-assembly approach, where the cells are coaxed to synthesise their own extracellular matrix (ECM)...
December 2017: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/29298786/compound-heterozygous-mutations-with-novel-missense-abca12-mutation-in-harlequin-ichthyosis
#13
Benny Kai Guo Loo, Melissa Jeanne Batilando, Ene Choo Tan, Mark Jean Aan Koh
Harlequin ichthyosis (HI) is the most severe form of autosomal recessive congenital ichthyosis, presenting at birth with distinctive facial features and thick, plate-like scales over the entire body. The abnormal skin barrier predisposes the patient to multiple complications, including dehydration and sepsis. Mortality rates of babies with HI have been greatly reduced since the introduction of systemic retinoid therapy. Mutations in ABCA12 have been found to lead to HI. Most of these mutations are truncation or deletion mutations in the conserved region of the protein, leading to severe loss of ABCA12 function...
January 3, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29247230/gene-activated-matrix-bone-marrow-derived-mesenchymal-stem-cells-constructs-regenerate-sweat-glands-like-structure-in-vivo
#14
Pranish Kolakshyapati, Xiuyuan Li, Chunye Chen, Mingxia Zhang, Weiqiang Tan, Lie Ma, Changyou Gao
It is a significant challenge to regenerate full-thickness skin defects with sweat glands. Various skin substitutes have been developed to resolve this issue with minimal success. In this study, to yield a novel construct for in situ regeneration of sweat glands, the collagen-chitosan porous scaffold was combined with Lipofectamine 2000/pDNA-EGF complexes to obtain the gene-activated scaffold (GAS), which was then seeded with bone marrow-derived mesenchymal stem cells (BM-MSCs). The porous scaffold functionalized as a reservoir for the incorporated gene complexes which were released in a sustained manner...
December 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29211404/management-of-long-segment-anterior-urethral-stricture-%C3%A2-8cm-using-buccal-mucosal-bm-graft-and-penile-skin-ps-flap-outcome-and-predictors-of-failure
#15
Gamal A Alsagheer, Atef Fathi, Mohamed Sayed Abdel-Kader, Ahmed M Hasan, Omar Mohamed, Osama Mahmoud, Ahmad Abolyosr
PURPOSE: To evaluate the surgical outcome and predictors of failure of substitution urethroplasty using either dorsal onlay buccal mucosal (BM) graft or ventral onlay penile skin flap (PS) for anterior urethral stricture ≥ 8cm. PATIENTS AND METHODS: Between March 2010 and January 2016, 50 patients with anterior urethral stricture ≥ 8 cm were treated at our hospital. The surgical outcome and success rate were assessed. The predictors of failure were analyzed using multivariate analysis...
December 7, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/29208214/proof-of-concept-of-a-new-autologous-skin-substitute-for-the-treatment-of-deep-wounds-in-dogs
#16
L Ramió-Lluch, S Cerrato, P Brazis, R M Rabanal, D Fondevila, A Puigdemont
Autologous skin grafts are effective for the repair of large skin wounds, but the availability of large amounts of skin is often limited. Through bioengineering, several autologous skin substitutes have been developed for use in human clinical practice. However, few skin substitutes are available for use in animals. The aim of this study was to develop and assess an engineered autologous skin substitute for the treatment of deep wounds in veterinary medicine. Canine keratinocytes and fibroblasts were isolated after double enzyme digestion from 8mm punch biopsies from four healthy Beagle dogs...
December 2017: Veterinary Journal
https://www.readbyqxmd.com/read/29207659/2-3-4-dihydroxybenzylidene-malononitrile-as-a-novel-anti-melanogenic-compound
#17
Bonggi Lee, Kyoung Mi Moon, Jong Seung Lim, Yeojin Park, Do Hyun Kim, Sujin Son, Hyoung Oh Jeong, Dae Hyun Kim, Eun Kyeong Lee, Ki Wung Chung, Hye Jin An, Pusoon Chun, Arnold Y Seo, Ju-Hye Yang, Bong-Seon Lee, Jin Yeul Ma, Won-Kyung Cho, Hyung Ryong Moon, Hae Young Chung
Tyrosinase is a key player in ultraviolet-induced melanogenesis. Because excessive melanin accumulation in the skin can induce hyperpigmentation, the development of tyrosinase inhibitors has attracted attention in cosmetic-related fields. However, side effects including toxicity and low selectivity have limited the use of many tyrosinase inhibitors in cosmetics. We synthesized 12 novel 2-(substituted benzylidene)malononitrile derivatives and investigated their anti-melanogenic activities. Of these 12 compounds, 2-(3, 4-dihydroxy benzylidene)malononitrile (BMN11) exhibited the strongest inhibitory activity against tyrosinase (IC50 = 17...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29207551/hyaluronan-based-nanohydrogels-as-effective-carriers-for-transdermal-delivery-of-lipophilic-agents-towards-transdermal-drug-administration-in-neurological-disorders
#18
Seong Uk Son, Jae-Woo Lim, Taejoon Kang, Juyeon Jung, Eun-Kyung Lim
We suggest a convenient nanoemulsion fabrication method to create hyaluronan (HA)-based nanohydrogels for effective transdermal delivery. First, hyaluronan-conjugated dodecylamine (HA-Do) HA-based polymers to load the lipophilic agents were synthesized with hyaluronan (HA) and dodecylamine (Do) by varying the substitution ratio of Do to HA. The synthetic yield of HA-Do was more than 80% (HA-Do (A): 82.7 ± 4.7%, HA-Do (B): 87.1 ± 3.9% and HA-Do (C): 81.4 ± 4.5%). Subsequently, nanohydrogels were fabricated using the nanoemulsion method...
December 4, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29207342/accelerated-skin-wound-healing-by-selective-11%C3%AE-hydroxylase-cyp11b1-inhibitors
#19
Juliette Emmerich, Chris J van Koppen, Jens L Burkhart, Roger T Engeli, Qingzhong Hu, Alex Odermatt, Rolf W Hartmann
Previous studies have shown that inhibition of cortisol biosynthesis in skin leads to accelerated wound healing. Here, pyridylmethyl pyridine type 11β-hydroxylase (CYP11B1) inhibitors were optimized for topical application to avoid systemic side effects. The resulting very potent, non-toxic CYP11B1 inhibitor 14 (IC50 = 0.8 nM) exhibited good selectivity over 11β-HSD1, CYP17A1 and CYP19A1. The compound showed high stability toward human plasma (t1/2= > 150 min, as a substitute for wound fluid) and low stability toward HLS9 (t1/2 = 19 min) for rapid metabolic clearance after absorption...
November 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29202478/%C3%AE-%C3%AE-tcr-recruits-the-syk-pi3k-axis-to-drive-proinflammatory-differentiation-program
#20
Ryunosuke Muro, Takeshi Nitta, Kenta Nakano, Tadashi Okamura, Hiroshi Takayanagi, Harumi Suzuki
γδT cells produce inflammatory cytokines and have been implicated in the pathogenesis of cancer, infectious diseases, and autoimmunity. The T cell receptor (TCR) signal transduction that specifically regulates the development of IL-17-producing γδT (γδT17) cells largely remains unclear. Here, we showed that the receptor proximal tyrosine kinase Syk is essential for γδTCR signal transduction and development of γδT17 in the mouse thymus. Zap70, another tyrosine kinase essential for the development of αβT cells, failed to functionally substitute for Syk in the development of γδT17...
December 4, 2017: Journal of Clinical Investigation
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