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Skin substitute

Sanne D'hondt, Brecht Guillemyn, Delfien Syx, Sofie Symoens, Riet De Rycke, Leen Vanhoutte, Wendy Toussaint, Bart N Lambrecht, Anne De Paepe, Douglas R Keene, Yoshihiro Ishikawa, Hans Peter Bächinger, Sophie Janssens, Mathieu J M Bertrand, Fransiska Malfait
Type III collagen is a major fibrillar collagen consisting of three identical α1 (III)-chains that is particularly present in tissues exhibiting elastic properties, such as the skin and the arterial wall. Heterozygous mutations in the COL3A1 gene result in vascular Ehlers-Danlos syndrome (vEDS), a severe, life-threatening disorder, characterized by thin, translucent skin and propensity to arterial, intestinal and uterine rupture. Most human vEDS cases result from a missense mutation substituting a crucial glycine residue in the triple helical domain of the α1 (III)-chains...
March 15, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Georg Dewald
Hereditary angioedema (HAE) is a genetically heterogeneous disease that is characterized by recurrent skin swelling, abdominal pain attacks, and potentially life-threatening upper airway obstruction. The two classic types, HAE types I and II, are both caused by mutations in the complement C1 inhibitor (SERPING1) gene resulting either in a quantitative or a qualitative deficiency of C1 inhibitor. In so-called HAE type III, in contrast, patients show normal C1 inhibitor measurements in plasma ('HAE with normal C1 inhibitor')...
March 25, 2018: Biochemical and Biophysical Research Communications
Danny Mangual, Luis A Bisbal-Matos, Ricardo Jiménez-Lee, Román Vélez, Miguel Noy
The case of a 27-year-old Hispanic female who presented with an occipito-parietal tumor after suffering trauma to the area. A physical examination revealed no tenderness to palpation and with evidence of healing ulcerations. The biopsy was consistent with a synovial sarcoma. A wide excision of the mass (15cm x 14cm x 6cm) followed by a pericranial flap was performed. A follow-up CT showed recurrence involving the parietal sagittal sinus. After a second biopsy the mass was determined to be a small-cell sarcoma, consistent with Ewing's sarcoma...
March 2018: Puerto Rico Health Sciences Journal
Johannes Wohlrab, Petra Staubach, Matthias Augustin, Lisa Eisert, Andreas Hünerbein, Alexander Nast, Holger Reimann, Klaus Strömer, Vera Mahler
The present guidelines are aimed at dermatology residents and board-certified dermatologists as well as policymakers and insurance companies. Developed by dermatologists in collaboration with pharmacists using a formal consensus process (S2k), they include general aspects with respect to pharmacokinetics and regulatory terminology. Recommendations are provided on the various indications for extemporaneous preparations and their quality assurance. The importance of pharmaceutical vehicles and problems associated with substituting one vehicle for another are discussed...
March 2018: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
Yousheng Yan, Zhaoyan Meng, Shengju Hao, Fang Wang, Xiaohua Jin, Daguang Sun, Huafang Gao, Xu Ma
BACKGROUND: Dystrophic epidermolysis bullosa (DEB) is an inherited skin disorder with variable severity and heterogeneous genetic involvement. Recessive DEB (RDEB) is a rare heritable blistering skin condition caused by loss-of-function mutations in the COL7A1 gene. AIM: This study aimed to determine the genetic basis of three Chinese RDEB patients from different families and identify correlations between phenotype and genotype. METHODS: All three patients were diagnosed with RDEB based on typical phenotype...
January 2018: Annals of Clinical and Laboratory Science
Mazaher Gholipourmalekabadi, Alexander M Seifalian, Aleksandra M Urbanska, Mir Davood Omrani, John G Hardy, Zahra Madjd, Seyed Mahmoud Hashemi, Hossein Ghanbarian, Peiman Brouki Milan, Masoud Mozafari, Rui L Reis, Subhas C Kundu, Ali Samadikuchaksaraei
Severe burn injuries can lead to delay in healing and devastating scar formation. Attempts are made to develop a suitable skin substitute for scarless healing of such skin wounds. Currently, there is no effective strategy yet for a complete scarless healing after the thermal injuries. In our recent work we fabricate and evaluated a 3D protein-based artificial skin made from decellularized human amniotic membrane (AM) and electrospun nanofibrous silk fibroin (ESF) in vitro. We also characterize both biophysical and cell culture investigation to establish in vitro performance of the developed bilayer scaffolds...
March 12, 2018: Biomacromolecules
Gautam Achar, C R Shahini, Siddappa A Patil, Jan Grzegorz Małecki, Szu-Hua Pan, Albert Lan, Xuan-Ren Chen, Srinivasa Budagumpi
In this contribution, a series of sterically-encumbered coumarin substituted benzimidazole-based N-heterocyclic carbene (NHC) precursors (1-12) and their silver(I)-NHC complexes (13-24) are reported. Molecular structure of NHC precursors 8 and 12 and cationic complexes 15 and 16 was established by single crystal X-ray diffraction method. The silver(I) complexes demonstrated various significant intramolecular agostic-like interactions operating between the metal center and the hydrogen atoms of the substituents alongside a variety of feeble π-π stacking interactions...
February 22, 2018: Journal of Inorganic Biochemistry
Haiying Li, Yuhan Xue, Bei Jia, Yun Bai, Yueyue Zuo, Shugeng Wang, Yanyan Zhao, Wenzhi Yang, Hongbo Tang
A series of hyaluronic acid grafted pullulan (HA-g-Pu) polymers with different hyaluronic acid (HA) moieties degrees of substitution (DS) were synthesized and characterized by fourier transform infrared (FT-IR), proton nuclear magnetic resonance (1 H NMR) and differential scanning calorimetry measurement (DSC). Compared to pure HA, HA-g-Pu polymers obtained better anti-enzymatic degradation ability in vitro, and the degradation rate of HA-g-Pu polymers depended on their different DS of HA moieties. The HA-g-Pu films were made of leaf-shape cascading arrangement with many small porous ranging from 0 to 100 μm in diameter when observed by scanning electron microscopy (SEM)...
May 15, 2018: Carbohydrate Polymers
Hee Jin Jung, Min Jung Lee, Yeo Jin Park, Sang Gyun Noh, A Kyoung Lee, Kyoung Mi Moon, Eun Kyeong Lee, Eun Jin Bang, Yun Jung Park, Su Jeong Kim, Jungho Yang, Sultan Ullah, Pusoon Chun, Young Suk Jung, Hyung Ryong Moon, Hae Young Chung
As part of continued efforts for the development of new tyrosinase inhibitors, (Z)-5-(substituted benzylidene)-2-iminothiazolidin-4-one derivatives (1a - 1l) were rationally synthesized and evaluated for their inhibitory potential in vitro. These compounds were designed and synthesized based on the structural attributes of a β-phenyl-α,β-unsaturated carbonyl scaffold template. Among these compounds, (Z)-5-(3-hydroxy-4-methoxybenzylidene)-2-iminothiazolidin-4-one (1e, MHY773) exhibited the greatest tyrosinase inhibition (IC50 = 2...
March 9, 2018: Bioscience, Biotechnology, and Biochemistry
Jeevitaa Kshersagar, Ravi Kshirsagar, Shashikant Desai, Raghvendra Bohara, Meghnad Joshi
Direct application of amnion has greater risk of immunological rejection and infection. Decellularization is an effective method to lower the risk of immune complications and infections. The bioreactor assembly with multiple cassettes was designed for decellurization of multiple amnions with different cell types simultaneously in single run. A detergent-based protocol was modified to remove all cellular components from amnion and diminish the DNA content to render it non-immunogenic. Amnion (n = 10) were treated with 2% sodium dodecyl sulphate (SDS), 5% dimethyl sulfoxide (DMSO) and 2% sodium deoxycholeate (SD)...
March 5, 2018: Cell and Tissue Banking
Thomas Später, Florian S Frueh, Philipp Karschnia, Michael D Menger, Matthias W Laschke
Dermal substitutes are frequently used for the initial coverage of extensive skin defects. The seeding of these implants with adipose tissue-derived microvascular fragments (ad-MVF) has recently been shown to accelerate their vascularization and incorporation. In the present study we analyzed whether these processes are affected by a thromboprophylactic therapy with the low molecular weight heparin (LMWH) enoxaparin (enox). Green fluorescent protein (GFP)+ ad-MVF were isolated from enox- (8 mg/kg s.c.) and vehicle-treated (0...
March 5, 2018: Wound Repair and Regeneration
Céline Thomet, Ali Modarressi, Eva Meia Rüegg, Pavel Dulguerov, Brigitte Pittet-Cuénod
Long-segment tracheal reconstruction remains a challenge. The ideal tracheal substitute should be an epithelialized tube to prevent stenosis and sufficiently rigid to maintain airflow patency. An autologous technique using a radial forearm free flap reinforced by rib cartilage has been recently described for tracheal reconstruction. We report here two cases of complex tracheal reconstruction with a modification of this technique, which consists of the creation of two independent skin paddles to allow the reconstruction of the trachea and a second adjacent defect (eg, cervical skin, esophagus)...
February 27, 2018: Annals of Plastic Surgery
Dhaval R Kalaria, Mayank Singhal, Vandana Patravale, Virginia Merino, Yogeshvar N Kalia
Effective treatment of Parkinson's disease (PD) involves administration of therapeutic agents with complementary mechanisms of action in order to replenish, sustain or substitute endogenous dopamine. The objective of this study was to investigate anodal co-iontophoresis of pramipexole (PRAM; dopamine agonist) and rasagiline (RAS; MAO-B inhibitor) in vitro and in vivo. Passive permeation of PRAM and RAS (20 mM each) across porcine skin after 6 h was 15.7±1.9 and 16.0±2.9 µg/cm2 , respectively. Co-iontophoresis at 0...
February 22, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Angelica Panico, Federica Paladini, Mauro Pollini
Skin injuries represent a health problem with consequences in terms of morbidity, disability and life quality. Numerous strategies have been developed for the treatment of wounds, including skin substitutes, biomembranes, scaffolds, and smart dressings. The excellent properties of fibroin can be exploited for the development of advanced wound dressing biomaterials, aiming at promoting the wound healing process. In this work, silk fibroin films modified through the addition of glucose were developed to enhance flexibility of medical device without affecting the biocompatibility, to promote wound healing and to improve the patient well-being...
February 16, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Mariam Mir, Murtaza Najabat Ali, Afifa Barakullah, Ayesha Gulzar, Munam Arshad, Shizza Fatima, Maliha Asad
Wounds are of a variety of types and each category has its own distinctive healing requirements. This realization has spurred the development of a myriad of wound dressings, each with specific characteristics. It is unrealistic to expect a singular dressing to embrace all characteristics that would fulfill generic needs for wound healing. However, each dressing may approach the ideal requirements by deviating from the 'one size fits all approach', if it conforms strictly to the specifications of the wound and the patient...
February 14, 2018: Progress in Biomaterials
A Dakiw Piaceski, D Larouche, K Ghani, F Bisson, S Cortez Ghio, S Larochelle, M J Moulin, M Caruso, L Germain
The combination of gene therapy and tissue engineering is one of the most promising strategies for the treatment of recessive dystrophic epidermolysis bullosa (RDEB). RDEB is a rare genetic disease characterised by mutations in the COL7A1 gene, encoding type VII collagen (COLVII), which forms anchoring fibrils at the dermal-epidermal junction of the skin. This disease causes severe blistering and only palliative treatments are offered. In this study, the base of a strategy combining gene therapy and a tissue-engineered skin substitute (TES), which would be suitable for the permanent closure of skin wounds, was set-up...
February 14, 2018: European Cells & Materials
Gangqiang Zhang, Xiangqiong Zeng, Yibo Su, F X Borras, Matthijn B de Rooij, Tianhui Ren, Emile van der Heide
The frictional performances of surgical sutures have been found to play a vital role in their functionality. The purpose of this paper is to understand the frictional performance of multifilament surgical sutures interacting with skin substitute, by means of a penetration friction apparatus (PFA). The influence of the size of the surgical suture was investigated. The relationship between the friction force and normal force was considered, in order to evaluate the friction performance of a surgical suture penetrating a skin substitute...
February 3, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Fabrizio Spada, Tanya M Barnes, Kerryn A Greive
Derivatives of hydrocortisone, such as mometasone furoate, a (2') furoate-17 ester with chlorine substitutions at positions 9 and 21, have been designed to improve efficacy and reduce the incidence of adverse effects. An extensive literature search of MEDLINE, Embase and other databases was conducted to review the safety and efficacy of various formulations of topical mometasone furoate. Mometasone furoate exhibits high potency with greater anti-inflammatory activity and a longer duration of action than betamethasone...
February 7, 2018: Australasian Journal of Dermatology
Phoebe F Lamie, John N Philoppes, Lucie Rárová
A new series of 1,2-diaryl-4-substituted-benzylidene-5(4H)-imidazolone derivatives 4a-l was synthesized. Their structures were confirmed by different spectroscopic techniques (IR, 1 H NMR, DEPT-Q NMR, and mass spectroscopy) and elemental analyses. Their cytotoxic activities in vitro were evaluated against breast, ovarian, and liver cancer cell lines and also normal human skin fibroblasts. Cyclooxygenase (COX)-1, COX-2 and lipoxygenase (LOX) inhibitory activities were measured. The synthesized compounds showed selectivity toward COX-2 rather than COX-1, and the IC50 values (0...
February 5, 2018: Archiv der Pharmazie
Dogeon Yoon, Dajeong Yoon, Hyuk-Jin Cha, Ji-Seon Lee, Wook Chun
The use of artificial dermis as a skin substitute represents a field of active study, as acellular dermal matrices from cadavers are susceptible to infection owing to their human origin. One such alternative dermal replacement scaffold, INSUREGRAF®, is derived primarily from extracellular matrix proteins such as collagen and elastin and has been clinically used to treat severe skin wounds such as burns. This scaffold has proven to be useful to minimize wound contraction and scar formation owing to its biocompatibility, interconnected pore structure, sufficient biodegradability, and suitable mechanical properties...
February 1, 2018: Biomedical Materials
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