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External tissue expander

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https://www.readbyqxmd.com/read/29695257/comparative-omics-and-feeding-manipulations-in-chicken-indicate-a-shift-of-the-endocrine-role-of-visceral-fat-towards-reproduction
#1
Susanne Bornelöv, Eyal Seroussi, Sara Yosefi, Sharon Benjamini, Shoval Miyara, Mark Ruzal, Manfred Grabherr, Nima Rafati, Anna-Maja Molin, Ken Pendavis, Shane C Burgess, Leif Andersson, Miriam Friedman-Einat
BACKGROUND: The mammalian adipose tissue plays a central role in energy-balance control, whereas the avian visceral fat hardly expresses leptin, the key adipokine in mammals. Therefore, to assess the endocrine role of adipose tissue in birds, we compared the transcriptome and proteome between two metabolically different types of chickens, broilers and layers, bred towards efficient meat and egg production, respectively. RESULTS: Broilers and layer hens, grown up to sexual maturation under free-feeding conditions, differed 4...
April 26, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29684429/simultaneous-in-vitro-generation-of-human-cd34-derived-dendritic-cells-and-mast-cells-from-non-mobilized-peripheral-blood-mononuclear-cells
#2
Pavla Taborska, Jirina Bartunkova, Daniel Smrz
Dendritic cells (DCs) and mast cells (MCs) are key players of the immune system, often coming in close proximity in peripheral tissues. The interplay of these cells is, however, still poorly understood especially with regards to human cells. The reason for that is the absence of a well established in vitro human cell-based study system that would allow a simultaneous preparation of both cell types. In this study, we show a method for simultaneous generation of DCs and MCs from CD34+ stem cell progenitors that were isolated from the non-adherent fraction of non-mobilized peripheral blood mononuclear cells of healthy donors...
April 20, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29676570/caznos-nd-3-emits-tissue-penetrating-near-infrared-light-upon-force-loading
#3
Lejing Li, Lothar Wondraczek, Lihua Li, Yu Zhang, Ye Zhu, Mingying Peng, Chuanbin Mao
Mechanoluminescent (ML) materials are mechano-optical converters that can emit light under an external mechanical stimulus. All the existing ML materials can only emit light from near ultraviolet to red, which is outside the near-infrared (NIR) windows desired for biomechanical imaging. No studies have been done on doping rare earth (RE) ions with photoluminescence (PL) in the NIR region into a compound to form a ML material that emits NIR light in response to an external force. Here, we show that doping RE ions with a NIR PL into an inorganic compound does not usually result in the formation of a NIR ML material, which can only be achieved in the combination of Nd3+ ions and a CaZnOS compound among the combinations we studied...
April 20, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29595729/discussion-external-volume-expansion-up-regulates-cxcl12-expression-and-enhances-mesenchymal-stromal-cell-recruitment-toward-expanded-prefabricated-adipose-tissue-in-rats
#4
Yvonne I Heit, Dennis P Orgill
No abstract text is available yet for this article.
April 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29553526/using-a-microfluidics-device-for-mechanical-stimulation-and-high-resolution-imaging-of-c-elegans
#5
Holger Fehlauer, Adam L Nekimken, Anna A Kim, Beth L Pruitt, Miriam B Goodman, Michael Krieg
One central goal of mechanobiology is to understand the reciprocal effect of mechanical stress on proteins and cells. Despite its importance, the influence of mechanical stress on cellular function is still poorly understood. In part, this knowledge gap exists because few tools enable simultaneous deformation of tissue and cells, imaging of cellular activity in live animals, and efficient restriction of motility in otherwise highly mobile model organisms, such as the nematode Caenorhabditis elegans. The small size of C...
February 19, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29485570/pulling-and-pushing-stem-cells-to-control-their-differentiation
#6
Nureddin Ashammakhi, Outi Kaarela, Patrizia Ferretti
Much has already been done to achieve precisely controlled and customised regenerative therapies. Thanks to recent advances made in several areas relevant to regenerative medicine including the use of stimuli-responsive materials, 4-dimensional biofabrication, inducible pluripotent stem cells, control of stem cell fate using chemical and physical factors, minimal access delivery, and information-communication technology. In this short perspective, recent advances are discussed with a focus on a recent report on the use of mechanical stretching of nanoparticle-laden stem cells by using external magnetic field to induce defined cardiac line differentiation...
May 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29476784/genome-wide-identification-evolution-and-expression-analysis-of-cyclic-nucleotide-gated-channels-in-tobacco-nicotiana-tabacum-l
#7
Zarqa Nawaz, Kaleem U Kakar, Raqeeb Ullah, Shizou Yu, Jie Zhang, Qing-Yao Shu, Xue-Liang Ren
Tobacco (Nicotiana tabacum) serve as the top leading commercial, non-food, and model crop worldwide. Cyclic nucleotide-gated channels (CNGCs) are ligand-gated, calcium-permeable, divalent, cation-selective channels, involved in important biological functions. Here, we systematically characterized thirty-five CNGC genes in the genome of Nicotiana tabacum, and classified into four phylogenetic groups. Evolutionary analysis showed that NtabCNGC family of N. tabacum originated from the parental genome of N. sylvestris and N...
February 21, 2018: Genomics
https://www.readbyqxmd.com/read/29466657/chemiluminescent-biosensors-for-detection-of-second-messenger-cyclic-di-gmp
#8
Andrew B Dippel, Wyatt A Anderson, Robert S Evans, Samuel Deutsch, Ming C Hammond
Bacteria colonize highly diverse and complex environments, from gastrointestinal tracts to soil and plant surfaces. This colonization process is controlled in part by the intracellular signal cyclic di-GMP, which regulates bacterial motility and biofilm formation. To interrogate cyclic di-GMP signaling networks, a variety of fluorescent biosensors for live cell imaging of cyclic di-GMP have been developed. However, the need for external illumination precludes the use of these tools for imaging bacteria in their natural environments, including in deep tissues of whole organisms and in samples that are highly autofluorescent or photosensitive...
March 5, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29408235/cell-extrusion-a-stress-responsive-force-for-good-or-evil-in-epithelial-homeostasis
#9
REVIEW
Shizue Ohsawa, John Vaughen, Tatsushi Igaki
Epithelial tissues robustly respond to internal and external stressors via dynamic cellular rearrangements. Cell extrusion acts as a key regulator of epithelial homeostasis by removing apoptotic cells, orchestrating morphogenesis, and mediating competitive cellular battles during tumorigenesis. Here, we delineate the diverse functions of cell extrusion during development and disease. We emphasize the expanding role for apoptotic cell extrusion in exerting morphogenetic forces, as well as the strong intersection of cell extrusion with cell competition, a homeostatic mechanism that eliminates aberrant or unfit cells...
February 5, 2018: Developmental Cell
https://www.readbyqxmd.com/read/29396297/in-vitro-regeneration-of-patient-specific-ear-shaped-cartilage-and-its-first-clinical-application-for-auricular-reconstruction
#10
Guangdong Zhou, Haiyue Jiang, Zongqi Yin, Yu Liu, Qingguo Zhang, Chen Zhang, Bo Pan, Jiayu Zhou, Xu Zhou, Hengyun Sun, Dan Li, Aijuan He, Zhiyong Zhang, Wenjie Zhang, Wei Liu, Yilin Cao
Microtia is a congenital external ear malformation that can seriously influence the psychological and physiological well-being of affected children. The successful regeneration of human ear-shaped cartilage using a tissue engineering approach in a nude mouse represents a promising approach for auricular reconstruction. However, owing to technical issues in cell source, shape control, mechanical strength, biosafety, and long-term stability of the regenerated cartilage, human tissue engineered ear-shaped cartilage is yet to be applied clinically...
February 2018: EBioMedicine
https://www.readbyqxmd.com/read/29394768/-a-case-report-of-post-radiochemotherapy-perineum-abscess-concurrent-with-recurrence-of-vaginal-cancer-for-which-total-pelvic-exenteration-was-performed
#11
Hiroki Imamura, Kohei Murata, Yoshinori Kagawa, Rieko Okubo, Takuya Sakamoto, Atsushi Naito, Kohei Murakami, Yoshiteru Katsura, Yoshiaki Ohmura, Atsushi Takeno, Shinichi Nakatsuka, Kimihiko Ito, Yutaka Takeda, Takeshi Kato, Shigeyuki Tamura
We report a case of a woman who was suffering from post-radiochemotherapy perineum abscess concurrent with the recurrence of vaginal cancer for which total pelvic exenteration was performed. A 66-year-oldwoman presentedat our hospital with irregular genital bleeding in November 2014. A series of examinations showedthat she was suffering from vaginal cancer(cT2N0M0, cStage II ). A radiochemotherapy regimen(external irradiation 45 Gy/25 Fr, CDDP 40mg/m2, 5 course)was commencedin January 2015. In the meantime, MRI revealedsome therapeutic effect, but in October 2015, MRI indicated the enlargement of the primary tumor(PD)...
November 2017: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/29327551/-analysis-of-the-axial-stretching-force-of-the-small-splint-fixation-system
#12
Hong-Gang Zhong, Wei-Hua Liu, Fu-Hui Dong
OBJECTIVE: To measure the axial stretching force produced by the small splint fixation system in the treatment of the fracture. METHODS: Dumbbell shaped plexiglass was machined to model the bone shaft with expanded two ends. Force transducer was set at the model fracture site to measure the stretching force produced by fracture fixation of the small splint with cloth aqueous bag that simulated the muscles and other soft tissue underneath. RESULTS: There was positive relationship between the axial stretching force produced at the model fracture site and the transverse pressing force exerted by the external plates...
January 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29322847/tissue-expanders-with-a-focus-on-extremity-reconstruction
#13
REVIEW
Abdul R Arain, Keegan Cole, Christopher Sullivan, Samik Banerjee, Jillian Kazley, Richard L Uhl
Acute traumatic or surgical wounds that cannot be primarily closed often cause substantial morbidity and mortality. This often leads to increased costs from higher material expenses, more involved nursing care, and longer hospital stays. Advancements in soft tissue expansion has made it a popular alternative to facilitate early closure without the need for more complicated plastic surgical procedures. Areas covered: In this review, we briefly elaborate on the history and biomechanics of tissue expansion and provide comprehensive descriptions of traditional internal tissue expanders and a variety of contemporary external tissue expanders...
February 2018: Expert Review of Medical Devices
https://www.readbyqxmd.com/read/29202272/fish-as-a-model-to-assess-chemical-toxicity-in-bone
#14
REVIEW
Ignacio Fernández, Paulo J Gavaia, Vincent Laizé, M Leonor Cancela
Environmental toxicology has been expanding as growing concerns on the impact of produced and released chemical compounds over the environment and human health are being demonstrated. Among the toxic effects observed in organisms exposed to pollutants, those affecting skeletal tissues (osteotoxicity) have been somehow overlooked in comparison to hepato-, immune-, neuro- and/or reproductive toxicities. Nevertheless, sub-lethal effects of toxicants on skeletal development and/or bone maintenance may result in impaired growth, reduced survival rate, increased disease susceptibility and diminished welfare...
January 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29064545/the-morphological-characters-of-the-male-external-genitalia-of-the-european-hedgehog-erinaceus-europaeus
#15
Ghasem Akbari, Mohammad Babaei, Nader Goodarzi
This study was conducted to depict anatomical characteristics of the penis in hedgehog. Seven sexually mature male European hedgehogs were used. Following anesthesia, the animals were scarified with chloroform inhalation. Gross penile characteristics such as length and diameter were thoroughly explored and measured using digital calipers. Tissue samples stained with Hematoxylin - Eosin and Masson's trichrome for microscopic analysis. The penis of the European hedgehog was composed of a pair of corpus cavernosum penis and the glans penis without corpus spongiosum penis...
October 24, 2017: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/29030904/development-and-maintenance-of-the-brain-s-immune-toolkit-microglia-and-non-parenchymal-brain-macrophages
#16
REVIEW
Jose P Lopez-Atalaya, Katharine E Askew K, Amanda Sierra, Diego Gomez-Nicola
Microglia and non-parenchymal macrophages located in the perivascular space, the meninges and the choroid plexus are independent immune populations that play vital roles in brain development, homeostasis, and tissue healing. Resident macrophages account for a significant proportion of cells in the brain and their density remains stable throughout the lifespan thanks to constant turnover. Microglia develop from yolk sac progenitors, later evolving through intermediate progenitors in a fine-tuned process in which intrinsic factors and external stimuli combine to progressively sculpt their cell-type specific transcriptional profiles...
October 14, 2017: Developmental Neurobiology
https://www.readbyqxmd.com/read/28841613/dedifferentiated-adipocytes-promote-adipose-tissue-generation-within-an-external-suspension-device
#17
Jingjing Ma, Yao Yao, Jing Wang, Ziqing Dong, Tao Zhou, Feng Lu, Yunjun Liao, Jianhua Gao
BACKGROUND: Mature adipocytes can dedifferentiate into fibroblast-like cells in vitro and acquire proliferation and redifferentiation/transdifferentiation abilities. A soft-tissue expander can induce adipocyte dedifferentiation in vivo. This study combined a tissue expander and an external suspension device to generate a large volume of adipose tissue. METHODS: A soft-tissue expander was implanted beneath the dorsal adipose flaps of rabbits. After 7 days of expansion, the expander was removed and an external suspension device was applied...
September 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28760620/microcontact-printing-of-polydopamine-on-thermally-expandable-hydrogels-for-controlled-cell-adhesion-and-delivery-of-geometrically-defined-microtissues
#18
Yu Bin Lee, Se-Jeong Kim, Eum Mi Kim, Hayeon Byun, Hyung-Kwan Chang, Jungyul Park, Yu Suk Choi, Heungsoo Shin
Scaffold-free harvest of microtissue with a defined structure has received a great deal of interest in cell-based assay and regenerative medicine. In this study, we developed thermally expandable hydrogels with spatially controlled cell adhesive patterns for rapid harvest of geometrically controlled microtissue. We patterned polydopamine (PD) on to the hydrogel via microcontact printing (μCP), in linear shapes with widths of 50, 100 and 200μm. The hydrogels facilitated formation of spatially controlled strip-like microtissue of human dermal fibroblasts (HDFBs)...
July 28, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28751248/reshapable-polymeric-hydrogel-for-controlled-soft-tissue-expansion-in-vitro-and-in-vivo-evaluation
#19
John Garner, Darrel Davidson, George J Eckert, Clark T Barco, Haesun Park, Kinam Park
Tissue expansion is the process by which extra skin is generated using a device that applies pressure from underneath the skin. Over the course of weeks to months, stretching by this pressure creates a flap of extra tissue that can be used to cover a defect area or enclose a permanent implant. Conventional tissue expanders require a silicone shell inflated either by external injections of saline solution or air, or by internal osmotic pressure generated by a hydrophilic polymer. In this study, a shell-free tissue expander comprised only of a chemically cross-linked biocompatible polymeric hydrogel is developed...
September 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28746132/three-dimensional-cross-platform-planning-for-complex-spinal-procedures-a-new-method-adaptive-to-different-navigation-systems
#20
Michael Kosterhon, Angelika Gutenberg, Sven R Kantelhardt, Jens Conrad, Amr Nimer Amr, Joachim Gawehn, Alf Giese
STUDY DESIGN: A feasibility study. OBJECTIVE: To develop a method based on the DICOM standard which transfers complex 3-dimensional (3D) trajectories and objects from external planning software to any navigation system for planning and intraoperative guidance of complex spinal procedures. SUMMARY OF BACKGROUND DATA: There have been many reports about navigation systems with embedded planning solutions but only few on how to transfer planning data generated in external software...
August 2017: Clinical Spine Surgery
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