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https://www.readbyqxmd.com/read/29470432/optical-method-for-estimating-the-chlorophyll-contents-in-plant-leaves
#1
Madaín Pérez-Patricio, Jorge Luis Camas-Anzueto, Avisaí Sanchez-Alegría, Abiel Aguilar-González, Federico Gutiérrez-Miceli, Elías Escobar-Gómez, Yvon Voisin, Carlos Rios-Rojas, Ruben Grajales-Coutiño
This work introduces a new vision-based approach for estimating chlorophyll contents in a plant leaf using reflectance and transmittance as base parameters. Images of the top and underside of the leaf are captured. To estimate the base parameters (reflectance/transmittance), a novel optical arrangement is proposed. The chlorophyll content is then estimated by using linear regression where the inputs are the reflectance and transmittance of the leaf. Performance of the proposed method for chlorophyll content estimation was compared with a spectrophotometer and a Soil Plant Analysis Development (SPAD) meter...
February 22, 2018: Sensors
https://www.readbyqxmd.com/read/29468396/vermicompost-dose-and-mycorrhization-determine-the-efficiency-of-copper-phytoremediation-by-canavalia-ensiformis
#2
Natielo Almeida Santana, Caroline Maria Rabuscke, Valdemir Bittencourt Soares, Hilda Hildebrand Soriani, Fernando Teixeira Nicoloso, Rodrigo Josemar Seminoti Jacques
The phytoremediation of copper (Cu)-contaminated sandy soils can be influenced by the addition of vermicompost to the soil and the mycorrhization of plants. The objective of this study was to evaluate the effects of inoculation with the mycorrhizal fungus Rhizophagus clarus and the addition of different doses of bovine manure vermicompost on the phytoremediation of a sandy soil with a high Cu content using Canavalia ensiformis. Soil contaminated with 100 mg kg-1 Cu received five doses of vermicompost and was cultivated with C...
February 21, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29437230/lectin-isolated-from-japanese-red-sword-beans-canavalia-gladiata-as-a-potential-cancer-chemopreventive-agent
#3
Satsuki Une, Koji Nonaka, Junich Akiyama
In this study, we investigated the chemical and biological profile of lectin isolated from Japanese red sword beans (Canavalia gladiata; RSBs). RSB lectin was purified using maltamyl-Sepharose 4B and subjected to amino acid composition and partial amino acid sequencing analyses, and evaluated for blood and carbohydrate specificity, mitogenic activity, splenic natural killer (NK) cell activity, and its effect on B16 melanoma cell proliferation, compared with Concanavalin A (Con A). The amino acid composition and sequences of RSB lectin were similar to those of Con A...
February 13, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29387380/nutritional-value-of-raw-canavalia-ensiformis-and-its-utilization-as-partial-replacement-for-soybean-meal-in-the-diet-of-clarias-gariepinus-burchell-1822-fingerlings
#4
Shola G Solomon, Victor T Okomoda, Obekpa Oguche
The nutritional value of raw Jack bean meal (Canavalia ensiformis) as a partial substitute for soybeans meal was investigated in this study. Preliminary investigation on nutrient composition revealed that lysine, histidine, and phenylalanine were significantly higher in C. ensiformis seed meal compared to soybean meal. However, crude protein and other essential amino acids were significantly lower. Feeding trial was then conducted to investigate the effect of replacing about 40% soybeans meal (at 58.8% inclusion) with C...
January 2018: Food Science & Nutrition
https://www.readbyqxmd.com/read/29374843/rearing-system-for-the-predatory-phytoseiid-euseius-concordis-acari-phytoseiidae
#5
Elias Soares de Figueiredo, Marcela Massaro, Stephanie do Carmo, Gilberto José de Moraes
Several species of predatory mites, especially those of the family Phytoseiidae, are potentially useful for the control of pest mites and insects. Among the phytoseiids, Euseius concordis (Chant), a species commonly found in South America, has been studied for possible use as biological control agent of pest mites. Given that Euseius species are known to feed on plant leaves and on pollen, the objective of this study was to determine plant species and pollen sources suitable to establish a pilot method of production of that species, based on a set of comparisons in the laboratory...
January 27, 2018: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/29276657/subtilisin-inhibitor-like-protein-pplpi-1-from-leaves-of-pigeonpea-cajanus-cajan-cv-bsmr-736-exhibits-inhibition-against-helicoverpa-armigera-gut-proteinases
#6
Faiyaz K Shaikh, Prafull P Gadge, Manohar V Padul, Manvendra S Kachole
Helicoverpa armigera is an orthodox rival of many crop plants affecting agricultural economy. Plant leaves found to accumulate proteinase inhibitors, although this insect pest chooses leaves for laying eggs. Plant defense response at this juncture is not fully explored. In this context, here we are reporting proteinase inhibitor (ppLPI-1) having significant homology with the I13 family from leaves of pigeonpea (cv. BSMR 736). The isolation of ppLPI-1 was carried out from leaves of field-grown pigeonpea under an outbreak of H...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29224356/heating-and-soaking-influence-the-in-vitro-hindgut-fermentation-in-pigs-of-tropical-legume-grains
#7
Julieta Torres, Luz Munoz, Michael Peters, Carlos Montoya
The effect of different thermal (raw versus autoclaving or boiling for 5 and 20 min) and soaking (with or without) treatments on the in vitro hindgut fermentation in pigs of undigested residue collected after in vitro foregut digestion of tropical legumes' grains (Canavalia brasiliensis; Lablab purpureus; pink, red and white Vigna unguiculata) were investigated. The undigested residue was fermented with a pig faecal inoculum to determine fermentability, gas and short-chain fatty acid (SCFA) productions. Soaked raw legumes increased the production of SCFAs (e...
December 9, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29191143/a-crude-sword-bean-canavalia-gladiata-extract-is-gelated-by-cooling
#8
Kaho Nishizawa, Yasuhiro Arii
White sword bean (Canavalia gladiata) seeds have the potential to be utilized in the manufacturing of processed foods owing to their high protein and carbohydrate content. Our previous reports explored the use of the sword bean as a source of food materials by preparing extracts in distilled water. In the present study, we found that one such extract can be gelated by cooling. The gelling substances were extracted by boiling and simultaneously stirring a suspension containing ground beans. Few proteins were present in the gelated extract...
December 1, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29189184/impact-of-electron-beam-irradiation-on-functional-attributes-of-seeds-of-two-coastal-wild-legume-landraces-of-canavalia
#9
Kandikere R Sridhar, Prabhavathi Supriya, Sudeep D Ghate
BACKGROUND: Vegetable proteins have widespread application in the food industry as functional ingredients in food formulations according to the recent patents. Requirement to develop less expensive protein-rich supplementary foods has resulted in shift of emphasis towards lesser known wild than popular legumes. OBJECTIVE: The aim of this study is to expose seeds of two coastal sand dune wild legumes of the Southwest India (Canavalia cathartica and C. maritima) to different doses of electron-beam (EB) irradiation to assess changes in functional attributes...
November 29, 2017: Recent Patents on Biotechnology
https://www.readbyqxmd.com/read/29156505/cross-reactivity-of-polyclonal-antibodies-against-canavalia-ensiformis-jack-bean-urease-and-h-pylori-urease-subunit-a-fragments
#10
Zbigniew Jerzy Kaminski, Inga Relich, Iwona Konieczna, Wieslaw Kaca, Beata Kolesinska
Overlapping decapeptide fragments of H. pylori urease subunit A (UreA) were synthesized and tested with polyclonal antibodies against Canavalia ensiformis (Jack bean) urease. The linear epitopes of UreA identified using the dot blot method were then examined using epitope mapping. For this purpose, series of overlapping fragments of UreA, frameshifted +/- 4 amino acid residues were synthesized. Most of the UreA epitopes which reacted with the Jack bean urease polyclonal antibodies had been recognized in previous studies by monoclonal antibodies against H...
November 20, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29114355/molecular-cytogenetic-characterization-and-comparison-of-the-two-cultivated-canavalia-species-fabaceae
#11
Chao-Wen She, Lin Wei, Xiang-Hui Jiang
The two cultivated Canavalia (Adanson, 1763) species, Canavalia gladiata (N. J. von Jacquin, 1788) A. P. de Candolle, 1825 and Canavalia ensiformis (Linnaeus, 1753) A. P. de Candolle, 1825 are closely related based on morphological and molecular phylogenetic data. However, the similarities and differences in genome organization between them have not been evaluated at molecular cytogenetic level. Here, detailed karyotypes of both species were constructed using combined PI and DAPI (CPD) staining, rDNA-FISH and self-genomic in situ hybridization (sGISH)...
2017: Comparative Cytogenetics
https://www.readbyqxmd.com/read/29025256/heterometal-coordinated-monomeric-concanavalin-a-at-ph-7-5-from-canavalia-ensiformis
#12
Nam-Jin Chung, Yeo Reum Park, Dong-Heon Lee, Jung Hee Park, Seung Jae Lee
The structure of concanavalin A (ConA) has been studied intensively due to its specific interactions with carbohydrates and its heterometal (Ca(2+) and Mn(2+)) coordination. Most structures from X-ray crystallography have shown ConA as a dimer or tetramer, because the complex formation requires specific crystallization conditions. Here, we reported a monomeric structure of ConA with a resolution of 1.6 Å, which revealed that metal coordination could trigger sugar binding ability. The calcium coordination residue, Asn14, changes the orientation of carbohydrate binding residues and biophysical details, including structural information, providing valuable clues for the development and application of detection kits using ConA...
October 13, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28926823/preparation-characterization-and-application-of-urease-nanoparticles-for-construction-of-an-improved-potentiometric-urea-biosensor
#13
Seema Jakhar, C S Pundir
The nanoparticles (NPs) aggregates of commercial urease from jack beans (Canavalia ensiformis) were prepared by desolvation and glutaraldehyde crosslinking and functionalized by cysteamine dihydrochloride. These enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM), UV and Fourier transform infrared (FTIR) spectroscopy. The TEM images of urease NPs showed their size in the range, 18-100nm with an average of 51.2nm. The ENPs were more active and stable with a longer shelf life than native enzyme molecules...
September 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28851635/a-novel-polyurethane-nano-zno-matrix-for-immobilization-of-chitinolytic-enzymes-and-optical-sensing-of-chitin
#14
Preety, Vinita Hooda
Purified chitinase from Vigna mungo and N-acetyl β glucosaminidase (NAGase) from Canavalia ensiformis were immobilized on to the novel polyurethane (PU)/zinc oxide nanoparticles (nano ZnO) composite matrix with a conjugation yield of 0.785±0.01mg/cm(2) and 96.19±0.85% retention of specific activity. Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) also confirmed the presence of nano ZnO and enzymes on the PU support. Thus synthesized PU/nano ZnO/chitinase/NAGase conjugates were optimized with respect to pH, temperature and substrate concentration and successfully employed for development of an absorbance based optical biosensor for chitin determination in stored wheat grains...
August 26, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28803976/canavalia-bonariensis-lectin-molecular-bases-of-glycoconjugates-interaction-and-antiglioma-potential
#15
Benildo Sousa Cavada, Mayara Torquato Lima Silva, Vinicius Jose Silva Osterne, Vanir Reis Pinto-Junior, Ana Paula Machado do Nascimento, Ingrid Alessandra Victoria Wolin, Isabella Aparecida Heinrich, Clareane Avelino Simplicio Nobre, Cleane Gomes Moreira, Claudia Figueiredo Lossio, Cintia Renata Costa Rocha, Jorge Luiz Martins, Kyria Santiago Nascimento, Rodrigo Bainy Leal
CaBo is a mannose/glucose-specific lectin purified from seeds of Canavalia bonariensis. In the present work, we report the CaBo crystal structure determined to atomic resolution in the presence of X-man, a specific ligand. Similar to the structural characteristics of other legume lectins, CaBo presented the jellyroll motif, a metal binding site occupied by calcium and manganese ions close to the carbohydrate-recognition domain (CRD). In vitro test of CaBo cytotoxicity against glioma cells demonstrated its ability to decrease the cellular viability and migration by induction of autophagy and cell death...
August 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28756174/the-potent-anti-cancer-activity-of-dioclea-lasiocarpa-lectin
#16
Ana C S Gondim, Isolda Romero-Canelón, Eduardo H S Sousa, Claudia A Blindauer, Jennifer S Butler, María J Romero, Carlos Sanchez-Cano, Bruno L Sousa, Renata P Chaves, Celso S Nagano, Benildo S Cavada, Peter J Sadler
The lectin DLasiL was isolated from seeds of the Dioclea lasiocarpa collected from the northeast coast of Brazil and characterized for the first time by mass spectrometry, DNA sequencing, inductively coupled plasma-mass spectrometry, electron paramagnetic resonance, and fluorescence spectroscopy. The structure of DLasiL lectin obtained by homology modelling suggested strong conservation of the dinuclear Ca/Mn and sugar-binding sites, and dependence of the solvent accessibility of tryptophan-88 on the oligomerisation state of the protein...
July 16, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28711300/raft-dependent-endocytic-movement-and-intracellular-cluster-formation-during-t%C3%A2-cell-activation-triggered-by-concanavalin-a
#17
Satomi Yabuuchi, Satoshi Endo, KeangOk Baek, Kunihide Hoshino, Yoshio Tsujino, Mun'delanji C Vestergaard, Masahiro Takagi
Certain food ingredients can stimulate the human immune system. A lectin, concanavalin A (ConA), from Canavalia ensiformis (jack bean) is one of the most well-known food-derived immunostimulants and mediates activation of cell-mediated immunity through T cell proliferation. Generally, T cell activation is known to be triggered by the interaction between T cells and antigen-presenting cells (APCs) via a juxtacrine (contact-dependent) signaling pathway. The mechanism has been well characterized and is referred to as formation of the immunological synapse (IS)...
July 12, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28693997/lectin-from-canavalia-villosa-seeds-a-glucose-mannose-specific-protein-and-a-new-tool-for-inflammation-studies
#18
Claudia F Lossio, Cleane G Moreira, Renata M F Amorim, Clareane S Nobre, Mayara T L Silva, Cornevile C Neto, Vanir R Pinto-Junior, Ivanice B Silva, Julia Campos, Ana Maria S Assreuy, Benildo S Cavada, Kyria S Nascimento
With important carbohydrate binding properties, lectins are proteins able to decipher the glycocode, and as such, they can be used in bioassays involving cell-cell communication, protein targeting, inflammation, and hypernociception, among others. In this study, a new glucose/mannose-specific lectin from Canavalia villosa seeds (Cvill) was isolated by a single affinity chromatography step in a Sephadex(®) G-50 column, with a purification yield of 19.35mg of lectin per gram of powdered seed. Analysis of intact protein by mass spectrometry showed the lectin is composed of three polypeptide chains, including a 25...
December 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28638403/jaburetox-update-on-a-urease-derived-peptide
#19
REVIEW
Arlete Beatriz Becker-Ritt, Camila Saretta Portugal, Célia Regina Carlini
Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. Urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kDa), which is released upon digestion of the protein by insect enzymes...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28602911/jaburetox-induced-toxic-effects-on-the-hemocytes-of-rhodnius-prolixus-hemiptera-reduviidae
#20
Natalia R Moyetta, Valquiria Broll, Ana Paula A Perin, Augusto F Uberti, Matheus V Coste Grahl, Fernanda Staniscuaski, Celia R Carlini, Leonardo L Fruttero
Jaburetox is a recombinant peptide derived from a Canavalia ensiformis urease that presents toxic effects upon several species of insects, phytopathogenic fungi and yeasts of medical importance. So far, no toxicity of Jaburetox to mammals has been shown. Previous reports have identified biochemical targets of this toxic peptide in insect models, although its mechanism of action is not completely understood. In this work, we aimed to characterize the effects of Jaburetox in hemolymphatic insect cells. For this purpose, the model insect and Chagas' disease vector Rhodnius prolixus was used...
June 8, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
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