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https://www.readbyqxmd.com/read/28428694/a-microarray-immunoassay-for-serum-thyrotropin-and-thyroglobulin-using-antibodies-immobilized-on-track-etched-membranes
#1
Bharti Jain, J Kumarasamy, Chandrakala Gholve, Savita Kulkarni, M G R Rajan
Serum thyroglobulin (Tg) and thyroid stimulating hormone (TSH) measurements have evolved as important analytes for monitoring the prognosis of patients with differentiated thyroid cancer, post-thyroidectomy. Individual analyte immunoassay is the current practice in clinical pathology, but the simultaneous assay for all relevant analytes for a given disease, can reduce assay costs, improve patient compliance and give the clinician more information for an unequivocal diagnosis. Microarray immunoassay (MI) can achieve this goal and, hence, we have developed and validated a immuno-radiometric MI for quantitation of serum TSH and Tg by using highly micro-porous polycarbonate (PC) track-etched membranes (TEM) to immobilize the monoclonal anti-TSH and polyclonal anti-Tg antibodies in ~1 mm diameter spots...
June 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28397878/a-novel-time-resolved-fluoroimmunoassay-for-the-quantitative-detection-of-antibodies-against-the-phospholipase-a2-receptor
#2
Biao Huang, Liang Wang, Yi Zhang, Jue Zhang, Qiuhua Zhang, Hualong Xiao, Bin Zhou, Zhuxing Sun, Ya-Nan Cao, Yu Chen, Zhigang Hu, Huiming Sheng
A highly sensitive time-resolved fluoroimmunoassay (TRFIA) was developed to quantify serum antibodies against the phospholipase A2 receptor (anti-PLA2R-IgG) for differential diagnosis of membranous nephropathy. Recombinant PLA2R (rPLA2R) was coated onto 96-well plates as a capture. A goat-anti-human IgG tracer was prepared with europium-chelate for detection. After bound/free separation by washing, the fluorescence counts of bound tracer were measured for quantifying serum anti-PLA2R-IgG concentration. A purified anti-PLA2R-IgG calibrator was first prepared for ensuring that consistent quantitative results could be obtained...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28343870/cd15-focus-score-infection-diagnosis-and-stratification-into-low-virulence-and-high-virulence-microbial-pathogens-in-periprosthetic-joint-infection
#3
V T Krenn, M Liebisch, B Kölbel, N Renz, T Gehrke, M Huber, M G Krukemeyer, A Trampuz, H Resch, V Krenn
INTRODUCTION: The aim of the work was to validate the CD15 focus score for the infection pathology of periprosthetic joint infection in a large group and to clarify whether a stratification into low-virulence and high-virulence microbial pathogens is possible by means of the CD15 focus score (quantification of CD15 positive granulocytes). METHODS: The histopathology of 275 synovial tissue samples taken intraoperatively during revision operations (n=127 hip, n=141 knee, n=2 shoulder, n=5 ankle) was evaluated according to the SLIM consensus classification (SLIM=synovial-like interface membrane)...
January 12, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28335787/risk-factors-of-atherosclerotic-tissue-types-in-single-vessel-and-intermediate-coronary-lesions-a-cross-sectional-study
#4
Xianjin Wang, Qun Chen, Yu Xu, Yanqing Wang, Yang Yang, Ming Gu, Haihua Xu, Yanfang Zhao
BACKGROUND: Few data exist that correlate lesion-related risk factors such as conventional cardiovascular risks or lipoprotein-associated phospholipase A2 (Lp-PLA2) with tissue types within atherosclerotic plaques in patients with single-vessel and intermediate coronary lesions. METHODS: One hundred and ninety-two patients with single-vessel and intermediate coronary lesions were enrolled in a cross-sectional study and divided into two groups: stable angina pectoris (SAP) and acute coronary syndrome (ACS) groups...
March 23, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28324708/removal-of-haloacetic-acids-from-swimming-pool-water-by-reverse-osmosis-and-nanofiltration
#5
Linyan Yang, Qianhong She, Man Pun Wan, Rong Wang, Victor W-C Chang, Chuyang Y Tang
Recent studies report high concentrations of haloacetic acids (HAAs), a prevalent class of toxic disinfection by-products, in swimming pool water (SPW). We investigated the removal of 9 HAAs by four commercial reverse osmosis (RO) and nanofiltration (NF) membranes. Under typical SPW conditions (pH 7.5 and 50 mM ionic strength), HAA rejections were >60% for NF270 with molecular weight cut-off (MWCO) equal to 266 Da and equal or higher than 90% for XLE, NF90 and SB50 with MWCOs of 96, 118 and 152 Da, respectively, as a result of the combined effects of size exclusion and charge repulsion...
March 10, 2017: Water Research
https://www.readbyqxmd.com/read/28319942/right-congenital-diaphragmatic-hernias-is-there-a-correlation-between-prenatal-lung-volume-and-postnatal-survival-as-in-isolated-left-diaphragmatic-hernias
#6
Teresa Victoria, Enrico Danzer, Edward R Oliver, J Christopher Edgar, Suzanne Iyoob, Emily A Partridge, Ann M Johnson, William H Peranteau, Beverly G Coleman, Alan W Flake, Mark P Johnson, Holly H Hedrick, N Scott Adzick
OBJECTIVES: Whereas left-sided congenital diaphragmatic hernias (L-CDH) have been extensively studied and their prognostic parameters delineated, right-sided hernias (R-CDH) have not. Published results remain inconclusive. The aim of this study is to evaluate if proven prognostic indicators of postnatal survival in the fetus with L-CDH apply to the fetus with R-CDH. METHODS: Retrospective single-center study of R-CDH fetuses with available prenatal studies assessed for fetal lung volume by means of ultrasound-measured observed versus expected (O/E) lung area to head circumference (LHR) and magnetic resonance-calculated O/E total lung volume (TLV) in a 12-year time period...
March 21, 2017: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/28319660/metal-organic-framework-uio-66-layer-a-highly-oriented-membrane-with-good-selectivity-and-hydrogen-permeance
#7
Sebastian Friebe, Benjamin Geppert, Frank Steinbach, Jürgen Caro
The 3D metal-organic framework (MOF) structure UiO-66 [Zr6O4(OH)4(bdc)6], featuring triangular pores of approximately 6 Å, has been successfully prepared as a thin supported membrane layer with high crystallographic orientation on ceramic α-Al2O3 supports. The adhesion of the MOF layer to the ceramic support was investigated in different taxing conditions. Furthermore, by coating this UiO-66 membrane with a thin polyimide (Matrimid) top layer, we prepared a multilayer composite. Said membranes have been evaluated in the separation of hydrogen (H2) from different binary mixtures at room temperature...
March 28, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28266628/evaluation-of-dynamic-changes-in-interstitial-fluid-proteome-following-microdialysis-probe-insertion-trauma-in-trapezius-muscle-of-healthy-women
#8
Maria V Turkina, Nazdar Ghafouri, Björn Gerdle, Bijar Ghafouri
Microdialysis (MD) has been shown to be a promising technique for sampling of biomarkers. Implantation of MD probe causes an acute tissue trauma and provokes innate response cascades. In order to normalize tissue a two hours equilibration period for analysis of small molecules has been reported previously. However, how the proteome profile changes due to this acute trauma has yet to be fully understood. To characterize the early proteome events induced by this trauma we compared proteome in muscle dialysate collected during the equilibration period with two hours later in "post-trauma"...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28222207/impact-of-the-type-of-dialysis-membranes-on-the-circulating-concentration-of-markers-of-vitamin-d-metabolism
#9
Etienne Cavalier, Pablo U Torres, Bernard E Dubois, Nicole Smelten, Hans Pottel, Jean-Marie Krzesinski, Pierre Delanaye
INTRODUCTION: Kidney Disease Improving Global Outcomes (KDIGO) guidelines recommend vitamin D supplementation in hemodialyzed patients to monitor 25(OH)-vitamin D 25(OH)D levels. However, patient-to-patient inconsistency can be observed in response to the treatment. In this study, we aimed to evaluate the impact of the dialysis membrane on 25(OH)D, albumin (Alb) and vitamin D-binding protein (VDBP), the major players of vitamin D transport and storage. MATERIAL AND METHODS: Alb (Cobas), VDBP (R&D) and 25(OH)D (liquid chromatography-tandem mass spectrometry) were measured in 75 patients before and after a 4-hour dialysis session...
February 18, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28214720/treatment-efficiency-and-economic-feasibility-of-biological-oxidation-membrane-filtration-and-separation-processes-and-advanced-oxidation-for-the-purification-and-valorization-of-olive-mill-wastewater
#10
L Ioannou-Ttofa, I Michael-Kordatou, S C Fattas, A Eusebio, B Ribeiro, M Rusan, A R B Amer, S Zuraiqi, M Waismand, C Linder, Z Wiesman, J Gilron, D Fatta-Kassinos
Olive mill wastewater (OMW) is a major waste stream resulting from numerous operations that occur during the production stages of olive oil. The resulting effluent contains various organic and inorganic contaminants and its environmental impact can be notable. The present work aims at investigating the efficiency of (i) jet-loop reactor with ultrafiltration (UF) membrane system (Jacto.MBR), (ii) solar photo-Fenton oxidation after coagulation/flocculation pre-treatment and (iii) integrated membrane filtration processes (i...
February 12, 2017: Water Research
https://www.readbyqxmd.com/read/28207795/organ-dysfunction-during-continuous-veno-venous-high-cut-off-hemodialysis-in-patients-with-septic-acute-kidney-injury-a-prospective-observational-study
#11
Gianluca Villa, Cosimo Chelazzi, Elena Morettini, Lucia Zamidei, Serafina Valente, A Lucia Caldini, Giovanni Zagli, A Raffaele De Gaudio, Stefano Romagnoli
BACKGROUND: Continuous veno-venous hemodialysis with high cut-off membranes (HCO-CVVHD) removes inflammatory mediators involved in organ dysfunction during sepsis. The aim of the present study was to assess the variations in SOFA score and identify early predictors of short-term mortality in a cohort of patients with septic shock, treated with HCO-CVVHD for acute kidney injury (AKI). METHODS: An observational prospective multicenter cohort study was conducted in four mixed medical-surgical ICUs...
2017: PloS One
https://www.readbyqxmd.com/read/28202790/reactivity-of-anti-pedv-structural-protein-antibodies-to-porcine-enteric-coronaviruses-diagnostic-implications
#12
Luis Gabriel Gimenez-Lirola, Jianqiang Zhang, Jose Antonio Carrillo-Avila, Qi Chen, Ronaldo Magtoto, Korakrit Poonsuk, David H Baum, Pablo Piñeyro, Jeffrey Zimmerman
The development of porcine epidemic diarrhea virus (PEDV) antibody-based assays is important for detecting infected animals, confirming previous virus exposure, and monitoring sow herd immunity. However, the potential cross-reactivity among porcine coronaviruses is a major concern for the development of pathogen-specific assays. In this study, we used serum samples (n = 792) from pigs of precisely known infection status and a multiplex fluorescent microbead-based immunoassay and/or enzyme-linked immunoassay platform to characterize the antibody response against PEDV whole-virus (WV) particles and recombinant polypeptides derived from the four PEDV structural proteins, i...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28166268/consequences-of-increasing-convection-onto-patient-care-and-protein-removal-in-hemodialysis
#13
Nathalie Gayrard, Alain Ficheux, Flore Duranton, Caroline Guzman, Ilan Szwarc, Fernando Vetromile, Chantal Cazevieille, Philippe Brunet, Marie-Françoise Servel, Àngel Argilés, Moglie Le Quintrec
INTRODUCTION: Recent randomised controlled trials suggest that on-line hemodiafiltration (OL-HDF) improves survival, provided that it reaches high convective volumes. However, there is scant information on the feasibility and the consequences of modifying convection volumes in clinics. METHODS: Twelve stable dialysis patients were treated with high-flux 1.8 m2 polysulphone dialyzers and 4 levels of convection flows (QUF) based on GKD-UF monitoring of the system, for 1 week each...
2017: PloS One
https://www.readbyqxmd.com/read/28101787/a-recombinant-highly-thermostable-%C3%AE-mannanase-retman26-from-thermophilic-bacillus-subtilis-tbs2-expressed-in-pichia-pastoris-and-its-ph-and-temperature-stability
#14
Zhangcai Luo, Jing Miao, Guoying Li, Yao Du, Xiaobin Yu
A gene encoding a highly thermostable β-mannanase from a thermophilic Bacillus subtilis (TBS2) was successfully expressed in Pichia pastoris. The maximum activity of the recombinant thermostable β-mannanase (ReTMan26) was 5435 U/mL, which was obtained by high-density, fed-batch cultivation after 168-h induction with methanol in a 50-L bioreactor. The protein yield reached 3.29 mg/mL, and the protein had a molecular weight of ~42 kDa. After fermentation, ReTMan26 was purified using a 10-kDa cut-off membrane and Sephadex G-75 column...
January 18, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28073845/agreement-between-programmed-cell-death-ligand-1-diagnostic-assays-across-multiple-protein-expression-cut-offs-in-non-small-cell-lung-cancer
#15
Marianne J Ratcliffe, Alan Sharpe, Anita Midha, Craig Barker, Marietta Scott, Paul Scorer, Hytham Al-Masri, Marlon Rebelatto, Jill Walker
PURPOSE: agents:Immunotherapies targeting programmed cell death-1 (PD-1) and programmed cell death ligand-1 (PD-L1) demonstrate encouraging antitumor activity and manageable tolerability in non-small cell lung cancer (NSCLC), especially in patients with high tumor PD-L1 expression, as detected by companion or complementary diagnostic assays developed for individual agents. A laboratory is unlikely to use multiple assay platforms. Furthermore, commercially available diagnostic assays are not standardized and different assay methods could lead to inappropriate treatment selection...
January 10, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28042837/purification-and-characterization-of-antioxidant-peptides-of-pseudosciaena-crocea-protein-hydrolysates
#16
Ningning Zhang, Chong Zhang, Yuanyuan Chen, Baodong Zheng
Two peptides with antioxidant activity were isolated from Pseudosciaena crocea proteins. Pseudosciaena crocea muscle was hydrolyzed with neutral protease to obtain Pseudosciaena crocea protein hydrolysates (PCPH). After ultrafiltration through molecular weight cut-off membranes of 10, 5 and 3 kDa and assessment of free radical scavenging ability, the fraction (PCPH-IV) with the highest antioxidant activity was obtained. Several purification steps, i.e., ion exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography, were applied to further purify PCPH-IV...
December 30, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27987984/biological-and-structural-analyses-of-bovine-heparin-fractions-of-intermediate-and-high-molecular-weight
#17
Alexsandro V Nogueira, Daiana L Drehmer, Marcello Iacomini, Guilherme L Sassaki, Thales R Cipriani
Low molecular weight heparin, which is generally obtained by chemical and enzymatic depolymerization of unfractionated heparin, has high bioavailability and can be subcutaneously injected. The aim of the present investigation was to fractionate bovine heparin using a physical method (ultrafiltration through a 10kDa cut-off membrane), avoiding structural modifications that can be caused by chemical or enzymatic treatments. Two fractions with different molecular weights were obtained: the first had an intermediate molecular weight (B-IMWH; Mn=9587Da) and the other had a high molecular weight (B-HMWH; 22,396Da)...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27888732/biorefinery-of-microalgal-soluble-proteins-by-sequential-processing-and-membrane-filtration
#18
C Safi, G Olivieri, R P Campos, N Engelen-Smit, W J Mulder, L A M van den Broek, L Sijtsma
A mild biorefinery process was investigated on the microalga Nannochloropsis gaditana, to obtain an enriched fraction of water soluble proteins free from chlorophyll. After harvesting, a 100g.L(-1) solution of cells was first subjected to cell disruption by either high-pressure homogenization (HPH) or enzymatic treatment (ENZ). HPH resulted in a larger release of proteins (49%) in the aqueous phase compared to the Alcalase incubation (35%). In both cases, an ultrafiltration/diafiltration (UF/DF) was then performed on the supernatant obtained from cell disruption by testing different membrane cut-off (1000kDa, 500kDa and 300kDa)...
February 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27887601/protocol-for-a-prospective-randomized-study-on-neurophysiological-assessment-of-lower-urinary-tract-function-in-a-healthy-cohort
#19
RANDOMIZED CONTROLLED TRIAL
Stéphanie van der Lely, Martina Stefanovic, Melanie R Schmidhalter, Marta Pittavino, Reinhard Furrer, Martina D Liechti, Martin Schubert, Thomas M Kessler, Ulrich Mehnert
BACKGROUND: Lower urinary tract symptoms are highly prevalent and a large proportion of these symptoms are known to be associated with a dysfunction of the afferent pathways. Diagnostic tools for an objective and reproducible assessment of afferent nerve function of the lower urinary tract are missing. Previous studies showed first feasibility results of sensory evoked potential recordings following electrical stimulation of the lower urinary tract in healthy subjects and patients. Nevertheless, a refinement of the methodology is necessary...
November 25, 2016: BMC Urology
https://www.readbyqxmd.com/read/27863531/effects-of-continuous-renal-replacement-therapy-on-linezolid-pharmacokinetic-pharmacodynamics-a-systematic-review
#20
Gianluca Villa, Paola Di Maggio, A Raffaele De Gaudio, Andrea Novelli, Riccardo Antoniotti, Enrico Fiaccadori, Chiara Adembri
BACKGROUND: Major alterations in linezolid pharmacokinetic/pharmacodynamic (PK/PD) parameters might be expected in critically ill septic patients with acute kidney injury (AKI) who are undergoing continuous renal replacement therapy (CRRT). The present review is aimed at describing extracorporeal removal of linezolid and the main PK-PD parameter changes observed in critically ill septic patients with AKI, who are on CRRT. METHOD: Citations published on PubMed up to January 2016 were systematically reviewed according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) statement...
November 19, 2016: Critical Care: the Official Journal of the Critical Care Forum
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