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High cut off membrane

Sonia R Lakhotia, Mausumi Mukhopadhyay, Premlata Kumari
In this paper, a new approach to synthesize thin-film nanocomposite membranes using cerium oxide (CeO2 ) nanoparticles (NPs) by pre-seeding interfacial polymerization method was reported. Prepared membranes were examined using contact angle, molecular weight cut-off (MWCO), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and scanning probe microscopy (SPM) to observe its hydrophilicity, pore size, morphology, surface chemistry, and roughness, respectively. Surface charges of the prepared membranes were also qualitatively calculated with the help of contact angle measurements by using the Grahame equation...
March 21, 2018: Scientific Reports
Odile Francesca Restaino, Maria Giovanna Borzacchiello, Ilaria Scognamiglio, Luigi Fedele, Alberto Alfano, Elena Porzio, Giuseppe Manco, Mario De Rosa, Chiara Schiraldi
BACKGROUND: Thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and could be potentially employed as bioremediation tools and bioscavengers. But nowadays their manufacturing in high yields is still an issue that limits their industrial applications. In this work we aimed to set up a high yield production and purification biotechnological process of two recombinant PLLs expressed in E. coli, the wild type SacPox from Sulfolobus acidocaldarius and a triple mutated SsoPox C258L/I261F/W263A, originally from Sulfolobus solfataricus...
March 20, 2018: BMC Biotechnology
Inga Markeviciute, Wolfgang Frenzel
Sample preparation is a key issue in the analysis of many real samples using ion chromatography (IC). One of the many means of sample preparation that has received considerable attention in recent years is the stopped-flow in-line dialysis approach hyphenated to IC. Using appropriate dialysis membranes undesirable high-molecular weight compounds, colloids and dispersed particulate matter are retained whereas the analyte ions pass through the membrane and are separated and quantified by IC. However, in the many papers about analytical use of in-line dialysis (not only in connection with IC) the retention of undesirable compounds has never been quantified...
August 3, 2018: Analytica Chimica Acta
Hitomi Mukaibo, Tonghui Wang, Victor H Perez-Gonzalez, Jirachai Getpreecharsawas, Jack T Wurzer, Blanca Lapizco-Encinas, James L McGrath
Insulator-based dielectrophoresis (iDEP) is a simple, scalable mechanism that can be used for directly manipulating particle trajectories in pore-based filtration and separation processes. However, iDEP manipulation of nanoparticles presents unique challenges as the dielectrophoretic force (<b><i>F</i></b><sub>DEP</sub>) exerted on the nanoparticles can easily be overshadowed by opposing kinetic forces. In this study, a molecularly thin, SiN-based nanoporous membrane (NPN) is explored as a breakthrough technology that enhances <b><i>F</i></b><sub>DEP</sub>...
March 12, 2018: Nanotechnology
Sara Bravaccini, Maurizio Puccetti, Martine Bocchini, Sara Ravaioli, Monica Celli, Emanuela Scarpi, Ugo De Giorgi, Maria Maddalena Tumedei, Giandomenico Raulli, Loredana Cardinale, Giovanni Paganelli
Prostate cancer (PCa) patients are risk-stratified on the basis of clinical stage and PSA level at diagnosis and the Gleason Score (GS) in prostate biopsy. However, these parameters are not completely accurate in discriminating between high- and low-risk disease, creating a need for a reliable marker to determine aggressiveness. Prostate-specific membrane antigen (PSMA) appears to fulfill this need. We analyzed 79 prostate biopsies and 28 prostatectomies to assess whether PSMA expression detected by immunohistochemistry is related to GS...
March 9, 2018: Scientific Reports
Davide Pierleoni, Matteo Minelli, Simone Ligi, Meganne Christian, Sebastian Funke, Niklas Reineking, Vittorio Morandi, Ferruccio Doghieri, Vincenzo Palermo
The performance of membranes for gas separation is currently limited by the Robeson limit, stating that it is impossible to have high gas permeability and high gas selectivity at the same time. We describe the production of membranes based on graphene oxide (GO) and poly(ethyleneimine) (PEI) multilayers able to overcome such limit. The PEI chains acts as molecular spacers in between the GO sheets, yielding a highly reproducible, periodic multi-layered structure with constant spacing of 3.7 nm, giving a record combination of gas permeability and selectivity...
March 9, 2018: ACS Applied Materials & Interfaces
Hatice Kansu-Celik, Yasemin Tasci, Burcu Kisa Karakaya, Mehmet Cinar, Tuba Candar, Gamze Sinem Caglar
OBJECTIVE: To evaluate the value of maternal serum advanced glycation end products (AGEs) level at 11-13 weeks' gestation for the prediction of preterm labor and or preterm premature rupture of membranes (PPROM). MATERIALS AND METHODS: This prospective cross-sectional study is performed in a university-affiliated hospital between February and April 2016. The participants of this study are low risk pregnant women. Blood samples for maternal AGEs level were collected in the first trimester of pregnancy and all women completed their antenatal follow-up and delivered in our center...
March 5, 2018: Journal of Maternal-fetal & Neonatal Medicine
Manisha Rewatkar, Shuchi Jain, Manish Jain, Kanchan Mohod
Prelabour rupture of the membranes (PLROM) is defined as rupture of membranes before the onset of labour. It is one of the most common clinical events, where pregnancy can turn into a high-risk situation for mother and foetus. As prevention of PLROM is difficult, one has to concentrate on management to reduce its complications. Accurate prediction of infection remains a main challenge in cases of PLROM. We conducted a prospective study of all women admitted for PLROM at or after 34-41 weeks of gestation to investigate the predictive value of C-reactive protein (CRP) and white blood cell (WBC) count for early-onset neonatal infection (EONI) and maternal chorioamnionitis...
February 12, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
Kevin Willy, Matthias Girndt, Jakob Voelkl, Roman Fiedler, Peter Martus, Markus Storr, Ralf Schindler, Daniel Zickler
BACKGROUND: Vascular calcification is a common phenomenon in patients with chronic kidney disease and strongly associated with increased cardiovascular mortality. Vascular calcification is an active process mediated in part by inflammatory processes in vascular smooth muscle cells (VSMC). These could be modified by the insufficient removal of proinflammatory cytokines through conventional high-flux (HF) membranes. Recent trials demonstrated a reduction of inflammation in VSMC by use of dialysis membranes with a higher and steeper cut-off...
December 22, 2017: Blood Purification
Ekaterina Stepanova, Steven P Gygi, Joao A Paulo
High-throughput proteomic profiling necessitates thorough optimization of all procedures to achieve a comprehensive analysis. We explore the use of a filter aided sample preparation method - termed Filter-based Protein Digestion (FPD) - in comparison to common chemical-based precipitation techniques for TMT whole proteome analysis. We divided a mammalian whole cell lysate for parallel protein processing with trichloroacetic acid (TCA; n=3), chloroform-methanol (C-M; n=2), and FPD using either 10 kDa (n=3) or 30 kDa (n=3) molecular weight cut-off filters...
February 5, 2018: Journal of Proteome Research
Antonio Santoro
In recent years, the population of chronic dialysis has grown in number but also in age and frequency of co-morbidies such as cardiac diseases, vascular pathologies, diabetes, etc. The majority of patients on chronic hemodialysis are over 70 years and, given the high number of comorbidities, they often exhibit poor tolerance to dialysis treatments. A non-tolerated dialytic treatment can have side-effects that would require an intensification of the dialysis sessions and many hospitalizations. Consequently, the problematic dialysis treatments, as well as harmful for the patient, become economically more detrimental than other treatments apparently more expensive but more tolerated ones In the current days we have, thanks to the huge developments in dialysis technology, powerful weapons to ensure effective and scarcely symptomatic dialysis treatments to the majority of the HD patients...
February 2018: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
Manuela A Hoffmann, Matthias Miederer, Helmut J Wieler, Christian Ruf, Frank M Jakobs, Mathias Schreckenberger
Background: Radiolabeled prostate-specific membrane antigen (PSMA) has proven to be a highly accurate method to detect recurrence and metastases of prostate cancer, but only sparse data is available about its performance in the diagnosis of clinically significant primary prostate cancer. Methods: We compared 68Ga-PSMA-11 PET/CT in 25 patients with 18FEC PET/CT in 40 patients with suspected prostate carcinoma based on an increased PSA level.The PET/CT results were compared with the histopathologic Gleason Score (GS) of biopsies...
December 19, 2017: Oncotarget
Luna Djirackor, Dilem Shakir, Helen Kalirai, Goran Petrovski, Sarah E Coupland
PURPOSE: Nestin, a member of the intermediate filament protein family, has been described as a putative cancer stem cell marker (CSC) in uveal melanoma and poor prognostic factor in a variety of tumours, including cutaneous melanoma. In this study, we examined the expression of nestin in primary (PUM) and metastatic uveal melanoma (MUM) samples, and correlated the findings with histological, clinical and survival data. METHODS: Nestin expression was assessed by immunohistochemistry in 141 PUM and 26 MUM samples; 11 PUM cases were matched with their corresponding metastases...
January 16, 2018: Acta Ophthalmologica
Eva Schepers, Griet Glorieux, Sunny Eloot, Michael Hulko, Adriana Boschetti-de-Fierro, Werner Beck, Bernd Krause, Wim Van Biesen
BACKGROUND: Membranes with increasing pore size are introduced to enhance removal of large uremic toxins with regular hemodialysis. These membranes might theoretically have higher permeability for bacterial degradation products. In this paper, permeability for bacterial degradation products of membranes of comparable composition with different pore size was investigated with a new in vitro set-up that represents clinical flow and pressure conditions. METHODS: Dialysis was simulated with an AK200 machine using a low-flux, high-flux, medium cut-off (MCO) or high cut-off (HCO) device (n = 6/type)...
January 5, 2018: BMC Nephrology
Ashraf Eldaly, Eman Omran, Mohamed A Youssef, Ahmed Abdallah, Ahmed Metwally, Hisham Haggag, Rasha Elkaffas
PURPOSE: The study aimed at assessment of the accuracy of the β-hCG test in vaginal washing fluid for diagnosis of prelabor rupture of membranes (PROM). PATIENTS AND METHODS: Two groups of pregnant women from 17 to 38 weeks of gestation were recruited. The first group (PROM group) included 50 pregnant women with unequivocal PROM. The other group included 50 pregnant women with intact membranes. A sterile speculum examination was performed. If less than 5 cc was collected or no fluid found, 10 cc sterile saline was sprinkled on the vaginal wall and 5 cc were recollected in a sterile syringe...
January 10, 2018: Journal of Maternal-fetal & Neonatal Medicine
Michelle R Ugolini-Lopes, Luciana Parente C Seguro, Maitê Xavier F Castro, Danielle Daffre, Alex C Lopes, Eduardo F Borba, Eloisa Bonfá
Objective: Two recent important lupus nephritis trials reported that proteinuria was a good predictor of renal outcome in Caucasians, but data on real-life situation, other races and severe nephritis are lacking to substantiate this finding as a simple test to guide clinical practice. The aim of this study was to validate proteinuria as a predictor of long-term renal outcome in real-life situation in a racially diverse group of patients with severe nephritis. Methods: Proteinuria, serum creatinine (SCr) and urine red blood cells were assessed at baseline and after 3, 6 and 12 months, as early predictors of long-term renal outcome (SCr <1...
2017: Lupus Science & Medicine
Liang Dong, Xiuhong Zhang, Fengming Liang, Xuan Yu, Ting Yang, Lang Li
OBJECTIVE: To investigate the prognostic value of oxygen challenge test (OCT) for patients with cardiogenic shock receiving extracorporeal membrane oxygenation (ECMO). METHODS: A retrospective analysis was conducted. Seventy-eight patients diagnosed with cardiogenic shock receiving veno-arterial (V-A) ECMO admitted to Department of intensive care unit (ICU) of Wuxi People's Hospital Affiliated to Nanjing Medical University from June 2012 to May 2017 were enrolled...
December 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Sung-Ju Im, Jungwon Choi, Jung-Gil Lee, Sanghyun Jeong, Am Jang
A new concept of volume-retarded osmosis and low-pressure membrane (VRO-LPM) hybrid process was developed and evaluated for the first time in this study. Commercially available forward osmosis (FO) and ultrafiltration (UF) membranes were employed in a VRO-LPM hybrid process to overcome energy limitations of draw solution (DS) regeneration and production of permeate in the FO process. To evaluate its feasibility as a water reclamation process, and to optimize the operational conditions, cross-flow FO and dead-end mode UF processes were individually evaluated...
March 2018: Chemosphere
Gang Han, Yingnan Feng, Tai-Shung Chung, Martin Weber, Christian Maletzko
This study has demonstrated the application of tight ultrafiltration (UF) membranes for effective removal of textile dyes from water at a low pressure. Novel UF hollow fiber membranes with well-defined nanopores and surface charges were developed via a single-step spinning process without any post-treatment. The newly developed tight UF hollow fibers not only possess a small mean pore diameter of 1.0-1.3 nm with a molecular weight cutoff (MWCO) of 1000-2000 Da but also have a high pure water permeability (PWP) of 82...
December 19, 2017: Environmental Science & Technology
Zaccaria Ricci, Stefano Romagnoli, Claudio Ronco
Innovation in continuous renal replacement therapies (CRRT) utilized to treat acute kidney injury (AKI) and sepsis, has brought new machines and techniques. Part of these new advances are due to the availability of innovative biomaterials and the construction of membranes with larger pores and wide distribution of pore sizes. This includes the creation of a new generation of high cut-off membranes whose utilization in clinical practice is promising for the wide spectrum of solutes that are removed during extracorporeal therapies...
November 24, 2017: International Journal of Artificial Organs
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