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https://www.readbyqxmd.com/read/28710997/dynamics-of-torque-teno-virus-plasma-dnaemia-in-allogeneic-stem-cell-transplant-recipients
#1
Eliseo Albert, Carlos Solano, Tania Pascual, Ignacio Torres, Lisa Macera, Daniele Focosi, Fabrizio Maggi, Estela Giménez, Paula Amat, David Navarro
BACKGROUND: Torque Teno virus (TTV) plasma DNA load directly correlate with the level of immunosuppresion in different clinical settings. It is uncertain whether this may be the case in allogeneic hematopoietic stem cell transplant recipients (allo-HSCT). OBJECTIVES: We characterized the dynamics of TTV DNAemia in patients undergoing T-cell replete allo-SCT. STUDY DESIGN: Retrospective single-center observational study including 72 allo-HSCT patients...
July 8, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28702856/dynamics-of-torque-teno-virus-viremia-could-predict-risk-of-complications-after-allogeneic-hematopoietic-stem-cell-transplantation
#2
Ramona Gilles, Marco Herling, Udo Holtick, Eva Heger, Sabine Awerkiew, Irina Fish, Konstantin Höller, Saleta Sierra, Elena Knops, Rolf Kaiser, Christof Scheid, Veronica Di Cristanziano
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an established treatment option for several hematological diseases. However, the first year post-transplantation is often complicated by infections and graft-versus-host disease (GVHD). Improvements in immunological monitoring could reduce such post-transplant complications. Torque Teno virus (TTV), a chronically persisting DNA virus, is reported to be a marker for immune function in immunocompromised patients. In the present study, the TTV kinetics were analyzed to investigate the potential role of TTV viremia as immune-competence read-out after allo-HSCT...
July 12, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28542207/metagenomic-sequencing-complements-routine-diagnostics-in-identifying-viral-pathogens-in-lung-transplant-recipients-with-unknown-etiology-of-respiratory-infection
#3
Dagmara W Lewandowska, Peter W Schreiber, Macé M Schuurmans, Bettina Ruehe, Osvaldo Zagordi, Cornelia Bayard, Michael Greiner, Fabienne D Geissberger, Riccarda Capaul, Andrea Zbinden, Jürg Böni, Christian Benden, Nicolas J Mueller, Alexandra Trkola, Michael Huber
BACKGROUND: Lung transplant patients are a vulnerable group of immunosuppressed patients that are prone to frequent respiratory infections. We studied 60 episodes of respiratory symptoms in 71 lung transplant patients. Almost half of these episodes were of unknown infectious etiology despite extensive routine diagnostic testing. METHODS: We re-analyzed respiratory samples of all episodes with undetermined etiology in order to detect potential viral pathogens missed/not accounted for in routine diagnostics...
2017: PloS One
https://www.readbyqxmd.com/read/28527409/molecular-characterization-of-pigeon-torque-teno-virus-pttv-in-jiangsu-province
#4
Zhicheng Zhang, Wei Dai, Dingzhen Dai
The torque teno virus (TTV) is a recently discovered DNA virus that has been detected in many different hosts, including humans, livestock and poultry. To date, there is no report of pigeon TTV (PTTV) from anywhere in the world. To investigate the distribution of PTTV in pigeons from the eastern Chinese province of Jiangsu and characterize their genomes, we employed PCR to detect PTTV in 144 samples collected from 6 pigeon plants in Jiangsu province, amplify complete genomes from representative samples and analyze genetic characteristics using bioinformatics...
May 6, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28489804/comparison-of-learning-performance-of-2-intubating-laryngeal-mask-airways-in-novice-a-randomized-crossover-manikin-study
#5
RANDOMIZED CONTROLLED TRIAL
Zi-Jia Liu, Jie Yi, Wei-Yun Chen, Xiu-Hua Zhang, Yu-Guang Huang
BACKGROUND: Intubating laryngeal mask airways (LMAs) such as i-gel and Aura-i could serve as rescue devices in resuscitation and further ensure the airway by facilitating trachea intubation without ventilation interruption. But data regarding intubating LMAs in novice are limited and skill degeneration without regular training has not been evaluated. So we designed this prospective randomized crossover manikin study to compare the learning performance of 2 intubating LMAs (i-gel and Aura-i)...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28482844/transplacental-transmission-of-torque-teno-virus
#6
Elena A Tyschik, Sophia M Shcherbakova, Ruslan R Ibragimov, Denis V Rebrikov
BACKGROUND: TTV has been detected in almost every human tissue type or body fluid reaching near 100% prevalence. Several studies report mother-to-child postnatal transmission of TTV in infancy but the risk of transplacental transmission of TTV is still unclear. METHODS: The blood and plasma collected postpartum from 100 mother-child pairs were analyzed using TTV-specific qPCR. Samples were collected from the peripheral vein of the mother and the umbilical cord. RESULTS: Eighty four percent of pregnant women were TTV positive (median titers: 8 × 10(4) copies/mL; range: 10(3) - 3 × 10(7))...
May 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28169509/nonalcoholic-fatty-liver-disease-and-measures-of-early-brain-health-in-middle-aged-adults-the-cardia-study
#7
Lisa B VanWagner, James G Terry, Lisa S Chow, Amy C Alman, Hakmook Kang, Katherine H Ingram, Christina Shay, Cora E Lewis, R Nick Bryan, Lenore J Launer, J Jeffrey Carr
OBJECTIVE: To assess associations between nonalcoholic fatty liver disease (NAFLD) and measures of brain health in a population-based sample of adults. METHODS: Participants from the CARDIA study (Y25 exam; age 43-55 years) with concurrent computed tomography quantification of liver fat, visceral adipose tissue (VAT), and brain magnetic resonance (MR) images were included (n = 505). NAFLD was identified after exclusion of other causes of liver fat. Total tissue volume (TTV) and gray matter cerebral blood flow (GM-CBF) were estimated using 3T brain MR images...
March 2017: Obesity
https://www.readbyqxmd.com/read/28130627/crescent-pyramid-and-drop-set-systems-do-not-promote-greater-strength-gains-muscle-hypertrophy-and-changes-on-muscle-architecture-compared-with-traditional-resistance-training-in-well-trained-men
#8
RANDOMIZED CONTROLLED TRIAL
Vitor Angleri, Carlos Ugrinowitsch, Cleiton Augusto Libardi
PURPOSE: The aim of this study was to compare the effects of crescent pyramid (CP) and drop-set (DS) systems with traditional resistance training (TRAD) with equalized total training volume (TTV) on maximum dynamic strength (1-RM), muscle cross-sectional area (CSA), pennation angle (PA), and fascicle length (FL). METHODS: Thirty-two volunteers had their legs randomized in a within-subject design in TRAD (3-5 sets of 6-12 repetitions at 75% 1-RM), CP (3-5 sets of 6-15 repetitions at 65-85% 1-RM), and DS (3-5 sets of ~50-75% 1-RM to muscle failure) protocols...
February 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28031027/prevalence-of-anelloviruses-ttv-ttmdv-and-ttmv-in-healthy-blood-donors-and-in-patients-infected-with-hbv-or-hcv-in-qatar
#9
Ahmed A Al-Qahtani, Enas S Alabsi, Raed AbuOdeh, Lukman Thalib, Gheyath K Nasrallah
BACKGROUND: Anelloviruses (TTV, TTMV, and TTMDV) have been associated with non A-G hepatitis. The goal of the current study was to estimate the prevalence of these anelloviruses in Qatar. METHODS: A total of 607 blood samples (500 healthy donors, and 53 HBV-and 54 HCV-positive patients) representing different nationalities were tested for the presence of TTV, TTMV, and TTMDV DNA by nested PCR. RESULTS: Prevalence rates for the three viruses were high in all studied groups, and exceeding 95% in the HBV group (for TTV and TTMDV)...
December 28, 2016: Virology Journal
https://www.readbyqxmd.com/read/27986348/updated-outcome-and-analysis-of-tumor-response-in-mobile-spine-and-sacral-chordoma-treated-with-definitive-high-dose-photon-proton-radiation-therapy
#10
Peyman Kabolizadeh, Yen-Lin Chen, Norbert Liebsch, Francis J Hornicek, Joseph H Schwab, Edwin Choy, Daniel I Rosenthal, Andrzej Niemierko, Thomas F DeLaney
PURPOSE: Treatment of spine and sacral chordoma generally involves surgical resection, usually in conjunction with radiation therapy. In certain circumstances where resection may result in significant neurologic or organ dysfunction, patients can be treated definitively with radiation therapy alone. Herein, we report the outcome and the assessment of tumor response to definitive radiation therapy. METHODS AND MATERIALS: A retrospective analysis was performed on 40 patients with unresected chordoma treated with photon/proton radiation therapy...
February 1, 2017: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/27924796/detection-and-phylogenetic-analysis-of-torque-teno-virus-in-salivary-and-tumor-biopsy-samples-from-head-and-neck-carcinoma-patients
#11
Andrea Hettmann, Anett Demcsák, Ádám Bach, Gábor Decsi, Ágnes Dencs, Dóra Pálinkó, László Rovó, Katalin Nagy, Janos Minarovits, Mária Takács
OBJECTIVES: Because torque teno virus (TTV) has been implicated in tumorigenesis as a cocarcinogen, we studied TTV prevalence in saliva and biopsy samples from head and neck cancer (HNCC) patients, patients with premalignant lesions of oral cancer, and controls. We also wished to determine the TTV genotypes in HNCC patients. METHODS: A seminested polymerase chain reaction (PCR) amplifying the N22 region of the TTV genome, as well as direct sequencing of PCR fragments, was used...
2016: Intervirology
https://www.readbyqxmd.com/read/27878979/prevalence-of-the-novel-torque-teno-sus-virus-species-k2b-from-pigs-in-the-united-states-and-lack-of-association-with-post-weaning-multisystemic-wasting-syndrome-or-mulberry-heart-disease
#12
A J Rogers, Y-W Huang, C L Heffron, T Opriessnig, A R Patterson, X-J Meng
The family Anelloviridae includes a number of viruses infecting humans (Torque teno viruses, TTV) and other animals including swine (Torque teno sus viruses, TTSuV). Two genetically distinct TTSuV species have been identified from swine thus far (TTSuV1 and TTSuVk2), although their definitive association with disease remains debatable. In 2012, a novel TTSuV species was identified from commercial swine serum and classified in the genus Kappatorquevirus as TTSuVk2b. The other Kappatorquevirus species, TTSuVk2a, has been associated with post-weaning multisystemic wasting syndrome (PMWS) when coinfected with porcine circovirus type 2 (PCV2)...
November 23, 2016: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/27851668/instability-resistance-training-improves-neuromuscular-outcome-in-parkinson-s-disease
#13
Carla Silva-Batista, Daniel Montie Corcos, Renato Barroso, Fabian J David, Hélcio Kanegusuku, Cláudia Forjaz, Marco Túlio DE Mello, Hamilton Roschel, Valmor Tricoli, Carlos Ugrinowitsch
PURPOSE: This study compared the effects of resistance training (RT) and RT with instability (RTI) on neuromuscular and total training volume (TTV) outcomes obtained as part of the Instability Resistance Training Trial in Parkinson's disease. It also used a linear multiple regression (forward stepwise method) to identify the contribution of neuromuscular outcomes to previously published improvements in the timed-up-and-go test and the Unified Parkinson's Disease Rating Scale, motor subscale score...
April 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/27836843/waterborne-viruses-and-f-specific-coliphages-in-mixed-use-watersheds-microbial-associations-host-specificities-and-affinities-with-environmental-land-use-factors
#14
Tineke H Jones, Julie Brassard, Edward Topp, Graham Wilkes, David R Lapen
From the years 2008 to 2014, a total of 1,155 water samples were collected (spring to fall) from 24 surface water sampling sites located in a mixed-used but predominantly agricultural (i.e., dairy livestock production) river basin in eastern Ontario, Canada. Water was analyzed for viable F-specific DNA (F-DNA) and F-specific RNA (F-RNA) (genogroup I [GI] to GIV) coliphage and a suite of molecularly detected viruses (norovirus [GI to GIV], torque teno virus [TTV], rotavirus, kobuvirus, adenovirus, astrovirus, hepatitis A, and hepatitis E)...
February 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27780187/predictors-of-successful-downstaging-of-hepatocellular-carcinoma-outside-milan-criteria
#15
Arvind R Murali, Carlos Romero-Marrero, Charles Miller, Federico Aucejo, Abraham Levitin, Amanjit Gill, Gordon McLennan, Karunakaravel Karuppasamy, Nizar N Zein, Rocio Lopez, K V Narayanan Menon
BACKGROUND: Patients with hepatocellular carcinoma (HCC) outside Milan criteria (MC) may be candidates for liver transplantation (LT) after successful downstaging. Factors that predict successful downstaging are unclear. We aimed to identify the predictors of successful downstaging of HCC in patients outside MC. METHODS: We performed a retrospective cohort study on consecutive patients with HCC outside MC who received downstaging with locoregional therapy. Clinical and laboratory variables, tumor characteristics including total tumor volume (TTV) and up-to-7 criteria were recorded...
November 2016: Transplantation
https://www.readbyqxmd.com/read/27731934/the-perioperative-lung-transplant-virome-torque-teno-viruses-are-elevated-in-donor-lungs-and-show-divergent-dynamics-in-primary-graft-dysfunction
#16
A A Abbas, J M Diamond, C Chehoud, B Chang, J J Kotzin, J C Young, I Imai, A R Haas, E Cantu, D J Lederer, K C Meyer, R K Milewski, K M Olthoff, A Shaked, J D Christie, F D Bushman, R G Collman
Primary graft dysfunction (PGD) is a principal cause of early morbidity and mortality after lung transplantation, but its pathogenic mechanisms are not fully clarified. To date, studies using standard clinical assays have not linked microbial factors to PGD. We previously used comprehensive metagenomic methods to characterize viruses in lung allografts >1 mo after transplant and found that levels of Anellovirus, mainly torque teno viruses (TTVs), were significantly higher than in nontransplanted healthy controls...
May 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/27699405/paucibacterial-microbiome-and-resident-dna-virome-of-the-healthy-conjunctiva
#17
Thuy Doan, Lakshmi Akileswaran, Dallin Andersen, Benjamin Johnson, Narae Ko, Angira Shrestha, Valery Shestopalov, Cecilia S Lee, Aaron Y Lee, Russell N Van Gelder
Purpose: To characterize the ocular surface microbiome of healthy volunteers using a combination of microbial culture and high-throughput DNA sequencing techniques. Methods: Conjunctival swab samples from 107 healthy volunteers were analyzed by bacterial culture, 16S rDNA gene deep sequencing (n = 89), and biome representational in silico karyotyping (BRiSK; n = 80). Swab samples of the facial skin (n = 42), buccal mucosa (n = 50), and environmental controls (n = 27) were processed in parallel...
October 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27633952/a-diverse-virome-in-kidney-transplant-patients-contains-multiple-viral-subtypes-with-distinct-polymorphisms
#18
Asha Rani, Ravi Ranjan, Halvor S McGee, Ahmed Metwally, Zahraa Hajjiri, Daniel C Brennan, Patricia W Finn, David L Perkins
Recent studies have established that the human urine contains a complex microbiome, including a virome about which little is known. Following immunosuppression in kidney transplant patients, BK polyomavirus (BKV) has been shown to induce nephropathy (BKVN), decreasing graft survival. In this study we investigated the urine virome profile of BKV+ and BKV- kidney transplant recipients. Virus-like particles were stained to confirm the presence of VLP in the urine samples. Metagenomic DNA was purified, and the virome profile was analyzed using metagenomic shotgun sequencing...
September 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27525643/torque-teno-virus-load-inverse-association-with-antibody-mediated-rejection-after-kidney-transplantation
#19
Martin Schiemann, Elisabeth Puchhammer-Stöckl, Farsad Eskandary, Philip Kohlbeck, Susanne Rasoul-Rockenschaub, Andreas Heilos, Nicolas Kozakowski, Irene Görzer, Željko Kikić, Harald Herkner, Georg A Böhmig, Gregor Bond
BACKGROUND: Antibody-mediated rejection (AMR) represents one of the cardinal causes of late allograft loss after kidney transplantation, and there is great need for noninvasive tools improving early diagnosis of this rejection type. One promising strategy might be the quantification of peripheral blood DNA levels of the highly prevalent and apathogenic Torque Teno virus (TTV), which might mirror the overall level of immunosuppression and thus help determine the risk of alloimmune response...
February 2017: Transplantation
https://www.readbyqxmd.com/read/27510954/detection-and-molecular-characterization-of-torque-teno-virus-ttv-in-uruguay
#20
Florencia Cancela, Natalia Ramos, Santiago Mirazo, Victoria Mainardi, Solange Gerona, Juan Arbiza
Torque Teno Virus (TTV), member of Anelloviridae family, is considered a worldwide distributed emergent virus and is currently classified into seven genogroups. Interestingly, the pathogenicity of TTV remains unclear. However, it has been constantly associated to hepatitis cases of unknown etiology (HUE) as well as extensively studied in concurrent infections with Hepatitis B Virus (HBV), Hepatitis C Virus (HCV) and Human Immunodeficiency Virus type 1 (HIV-1). In South America, TTV epidemiological data is scant, involving some studies from Brazil, Venezuela, Colombia and Bolivia...
October 2016: Infection, Genetics and Evolution
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