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https://www.readbyqxmd.com/read/28493527/characterization-of-a-sandwich-elisa-for-the-quantification-of%C3%A2-all-human-periostin-isoforms
#1
Elisabeth Gadermaier, Manfred Tesarz, Andreea Ana-Maria Suciu, Jacqueline Wallwitz, Gabriela Berg, Gottfried Himmler
BACKGROUND: Periostin (osteoblast-specific factor OSF-2) is a secreted protein occurring in seven known isoforms, and it is involved in a variety of biological processes in osteology, tissue repair, oncology, cardiovascular and respiratory systems or allergic manifestations. To analyze functional aspects of periostin, or the ability of periostin as potential biomarker in physiological and pathological conditions, there is the need for a precise, well-characterized assay that detects periostin in peripheral blood...
May 11, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28483425/risk-assessment-using-cytochrome-p450-time-dependent-inhibition-assays-at-single-time-and-concentration-in-the-early-stage-of-drug-discovery
#2
Mai Kosaka, Yohei Kosugi, Hideki Hirabayashi
In this article, we proposed a risk assessment strategy for CYP3A time-dependent inhibition (TDI) during drug discovery based on a through retrospective study of 13 reference drugs, some of which are known to have in vitro TDI potential, but have unknown clinical relevance. First, the traditional parameter kinact/KI, recommended by regulatory authorities for necessity decision-making in clinical drug-drug interaction (DDI) studies, was investigated as a predictive index for clinical TDI liability. The cutoff value of 1...
May 5, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28465155/effect-of-liver-disease-on-hepatic-transporter-expression-and-function
#3
REVIEW
Nilay Thakkar, Jason R Slizgi, Kim L R Brouwer
Liver disease can alter the disposition of xenobiotics and endogenous substances. Regulatory agencies such as the Food and Drug Administration (FDA) and the European Medicines Evaluation Agency (EMEA) recommend, if possible, studying the effect of liver disease on drugs under development to guide specific dose recommendations in these patients. While extensive research has been conducted to characterize the effect of liver disease on drug-metabolizing enzymes, emerging data have implicated that the expression and/or function of hepatobiliary transport proteins also are altered in liver disease...
April 29, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28272979/efficacy-of-phase-3-trial-of-rts-s-as01-malaria-vaccine-the-need-for-an-alternative-development-plan
#4
Shima Mahmoudi, Hossein Keshavarz
Although vaccines would be the ideal tool for control, prevention, elimination, and eradication of many infectious diseases, developing of parasites vaccines such as malaria vaccine is very complex. The most advanced malaria vaccine candidate RTS,S, a pre-erythrocytic vaccine, has been recommended for licensure by EMEA. The results of this phase III trial suggest that this candidate malaria vaccine has relatively little efficacy, and the vaccine apparently will not meet the goal of malaria eradication by itself...
March 8, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28064422/improved-detection-of-mineral-oil-toxicity-using-an-extended-mouse-embryo-assay
#5
Alessandra J Ainsworth, Jolene R Fredrickson, Dean E Morbeck
PURPOSE: Successful in vitro fertilization (IVF) relies on sound laboratory methods and culture conditions which depend on sensitive quality control (QC) testing. This study aimed to improve the sensitivity of mouse embryo assays (MEA) for detection of mineral oil toxicity. METHODS: Five experiments were conducted to study modifications of the standard mouse embryo assay (MEA) in order to improve sensitivity using clinical grade mineral oil with known peroxide concentrations...
March 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27884198/use-of-poly-adp-ribose-polymerase-parp-inhibitors-in-cancer-cells-bearing-ddr-defects-the-rationale-for-their-inclusion-in-the-clinic
#6
REVIEW
Aniello Cerrato, Francesco Morra, Angela Celetti
BACKGROUND: DNA damage response (DDR) defects imply genomic instability and favor tumor progression but make the cells vulnerable to the pharmacological inhibition of the DNA repairing enzymes. Targeting cellular proteins like PARPs, which cooperate and complement molecular defects of the DDR process, induces a specific lethality in DDR defective cancer cells and represents an anti-cancer strategy. Normal cells can tolerate the DNA damage generated by PARP inhibition because of an efficient homologous recombination mechanism (HR); in contrast, cancer cells with a deficient HR are unable to manage the DSBs and appear especially sensitive to the PARP inhibitors (PARPi) effects...
November 24, 2016: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/27802918/high-throughput-assay-for-quantification-of-the-plasma-concentrations-of-thiopental-using-automated-solid-phase-extraction-spe-directly-coupled-to-lc-ms-ms-instrumentation
#7
Seyed Mojtaba Moosavi, Kiran Shekar, John Fraser, Maree T Smith, Sussan Ghassabian
Most previous assays for thiopental are time-consuming due to laborious sample extraction steps prior to analysis using gas chromatography or high pressure liquid chromatography. Here, we describe the first high-throughput liquid chromatography - tandem mass spectrometry (LC-MS/MS) method for quantification of thiopental concentrations in samples of human plasma. Robotic on-line solid phase extraction (SPE) was used to elute the analytes of interest from samples of human plasma (50μL) loaded onto C18 SPE cartridges to which were added aliquots (50μL) of internal standard solution (thiopental-d5 100ng/mL) and 0...
December 1, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27694429/determination-of-nickel-and-manganese-contaminants-in-pharmaceutical-iron-supplements-using-energy-dispersive-x-ray-fluorescence
#8
Pedro Cardoso, Pedro Amaro, José Paulo Santos, Joaquim T de Assis, Maria Luisa Carvalho
In this study, we investigate the capability of energy dispersive X-ray fluorescence (EDXF) spectrometry in a triaxial geometry apparatus as a fast and nondestructive determination method of both dominant and contaminant elements in pharmaceutical iron supplements. The following iron supplements brands with their respective active ingredients were analyzed: Neutrofer fólico (iron gylcinate), Anemifer (iron(II) sulfate monohydrate), Noripurum (iron(III)-hydroxide polymaltose complex), Sulferbel (iron(II) sulfate monohydrate), and Combiron Fólico (carbonyl iron)...
March 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/27554158/biocide-and-antibiotic-resistance-of-enterococcus-faecalis-and-enterococcus-faecium-isolated-from-the-swine-meat-chain
#9
Lucia Rizzotti, Franca Rossi, Sandra Torriani
In this study nine strains of Enterococcus faecalis and 12 strains of Enterococcus faecium, isolated from different sample types in the swine meat chain and previously characterized for the presence of antibiotic resistance genes, were examined for phenotypic tolerance to seven biocides (chlorexidine, benzalkonium chloride, triclosan, sodium hypochlorite, 2-propanol, formaldehyde and hydrogen peroxide) and resistance to nine antibiotics (ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol)...
December 2016: Food Microbiology
https://www.readbyqxmd.com/read/27551429/efficacy-of-varicella-vzv-vaccination-an-update-for-the-clinician
#10
Lili Wang, Lucy Zhu, Hua Zhu
Varicella-zoster virus (VZV) infection causes two distinct clinical conditions. Primary varicella infection results in chickenpox, a contagious rash illness typically seen among children. VZV can reactivate years after the initial infection to cause herpes zoster (HZ) and lead to post-herpetic neuralgia, a common complication resulting in persistent pain that may last for years after the zoster rash resolves. A person's risk of having longer lasting and more severe pain associated with HZ increases with age...
January 2016: Therapeutic Advances in Vaccines
https://www.readbyqxmd.com/read/27508598/-op-lb03-09-the-acute-effect-of-the-specific-creatine-kinase-inhibitor-beta-gpa-in-healthy-man-abc-trial-a-randomized-placebo-and-active-controlled-first-in-human-trial
#11
F A Karamat, D Horjus, Y Haan, L Van Der Woude, M Schaap, I Oudman, G Van Montfrans, R Nieuwland, J Clark, A Sturk, L Brewster
OBJECTIVE: There is increasing evidence that the ATP generating enzyme creatine kinase (CK) is involved in hypertension. The enzyme is central to the regeneration of ATP by using creatine phosphate and ADP, thereby forming creatine and ATP. We recently showed that beta-guanidinopropionic acid (GPA), a creatine analogue and competitive CK inhibitor, effectively and safely reduced blood pressure in the spontaneously hypertensive rat. This renders GPA to be potentially beneficial for blood pressure lowering...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27474528/use-of-pamidronate-for-osteoporosis-treatment-in-public-health-care-in-brazil
#12
Leila Bianchet Zanatta, Cristina Marcatto, Cassio Slompo Ramos, Nadila Mañas, Carolina Moreira, Victoria Borba
PURPOSE: The use of bisphosphonates for osteoporosis is effective in reducing the risk of fractures. However, oral formulations are sometimes not well tolerated or are contraindicated. Due to its availability in Brazilian public health system, pamidronate is frequently prescribed for osteoporosis, despite the lack of studies demonstrating its anti-fracture efficacy and the absence of FDA or EMEA approval for this purpose. The aim of this study was to evaluate the bone mineral density (BMD) response to pamidronate in a group of women with osteoporosis in a tertiary care hospital...
July 7, 2016: Revista Brasileira de Reumatologia
https://www.readbyqxmd.com/read/26947494/sphingosine-1-phosphate-receptor-1-agonists-a-patent-review-2013-2015
#13
REVIEW
Miguel Guerrero, Mariangela Urbano, Edward Roberts
INTRODUCTION: The sphingosine-1-phosphate (S1P) regulates diverse biological functions including cell proliferation, endothelial cell chemotaxis, angiogenesis, immune cell trafficking, mitogenesis, heart rate. The first-in-class S1P1,3-5-R pan-agonist fingolimod (FTY720) was approved by the FDA and EMEA for the treatment of relapsing-remitting multiple sclerosis, though the most common adverse effect is bradycardia which occurs in the early stage of treatment and resolves within the first 24 h despite continuing treatment...
2016: Expert Opinion on Therapeutic Patents
https://www.readbyqxmd.com/read/26921826/pbt-assessment-and-prioritization-of-contaminants-of-emerging-concern-pharmaceuticals
#14
Alessandro Sangion, Paola Gramatica
The strong and widespread use of pharmaceuticals, together with incorrect disposal procedures, has recently made these products contaminants of emerging concern (CEC). Unfortunately, little is known about pharmaceuticals' environmental behaviour and ecotoxicity, so that EMEA (European Medicines Agency) released guidelines for the pharmaceuticals' environmental risk assessment. In particular, there is a severe lack of information about persistence, bioaccumulation and toxicity (PBT) of the majority of the thousands of substances on the market...
May 2016: Environmental Research
https://www.readbyqxmd.com/read/26905265/review-of-the-clinical-and-economic-burden-of-antibody-mediated-rejection-in-renal-transplant-recipients
#15
REVIEW
Gorden Muduma, Isaac Odeyemi, Jayne Smith-Palmer, Richard F Pollock
UNLABELLED: Antibody-mediated rejection (AbMR) is a leading cause of late graft loss in kidney transplant recipients, accounting for up to 60% of late graft failures. AbMR manifests as two distinct phenotypes: the first occurs in the immediate post-transplant period in sensitized patients; the second occurs in the late post-transplant period and has been associated with non-adherence to immunosuppression. The present review summarizes the current treatment options for AbMR, its clinical and economic burden, and approaches for reducing the risk of AbMR...
March 2016: Advances in Therapy
https://www.readbyqxmd.com/read/26834967/nurses-perceptions-of-medication-adherence-in-schizophrenia-results-of-the-adhes-cross-sectional-questionnaire-survey
#16
Robin Emsley, Koksal Alptekin, Jean-Michel Azorin, Fernando Cañas, Vincent Dubois, Philip Gorwood, Peter M Haddad, Dieter Naber, José Manuel Olivares, Georgios Papageorgiou, Miguel Roca, Pierre Thomas, Ludger Hargarter, Andreas Schreiner
OBJECTIVES: Poor adherence to antipsychotic treatment is a widespread problem within schizophrenia therapy with serious consequences including increased risks of relapse and rehospitalization. Mounting evidence supports the key roles that nurses play in monitoring patient progress and facilitating long-term treatment adherence. The Adherencia Terapéutica en la Esquizofrenia (ADHES) nurses' survey was designed to assess the opinions of nurses on the causes and management of partial/nonadherence to antipsychotic medication...
December 2015: Therapeutic Advances in Psychopharmacology
https://www.readbyqxmd.com/read/26669612/quantitation-of-brinzolamide-in-dried-blood-spots-by-a-novel-lc-qtof-ms-ms-method
#17
Anargyros Foivas, Anđelija Malenović, Nađa Kostić, Marija Božić, Miroslav Knežević, Yannis L Loukas, Yannis Dotsikas
In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0...
February 5, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/26617328/telemedicine-as-a-special-case-of-machine-translation
#18
Krzysztof Wołk, Krzysztof Marasek, Wojciech Glinkowski
Machine translation is evolving quite rapidly in terms of quality. Nowadays, we have several machine translation systems available in the web, which provide reasonable translations. However, these systems are not perfect, and their quality may decrease in some specific domains. This paper examines the effects of different training methods when it comes to Polish-English Statistical Machine Translation system used for the medical data. Numerous elements of the EMEA parallel text corpora and not related OPUS Open Subtitles project were used as the ground for creation of phrase tables and different language models including the development, tuning and testing of these translation systems...
December 2015: Computerized Medical Imaging and Graphics: the Official Journal of the Computerized Medical Imaging Society
https://www.readbyqxmd.com/read/26555843/is-the-step-wise-tiered-approach-for-era-of-pharmaceuticals-useful-for-the-assessment-of-cancer-therapeutic-drugs-present-in-marine-environment
#19
G V Aguirre-Martínez, C Okello, M J Salamanca, C Garrido, T A Del Valls, M L Martín-Díaz
Methotrexate (MTX) and tamoxifen (TMX) cancer therapeutic drugs have been detected within the aquatic environment. Nevertheless, MTX and TMX research is essentially bio-medically orientated, with few studies addressing the question of its toxicity in fresh water organisms, and none to its' effect in the marine environment. To the authors' knowledge, Environmental Risk Assessments (ERA) for pharmaceuticals has mainly been designed for freshwater and terrestrial environments (European Medicines Agency-EMEA guideline, 2006)...
January 2016: Environmental Research
https://www.readbyqxmd.com/read/26210585/a-novel-fully-validated-lc-ms-ms-method-for-quantification-of-pyridoxal-5-phosphate-concentrations-in-samples-of-human-whole-blood
#20
Sussan Ghassabian, Lyn Griffiths, Maree T Smith
Quantification of pyridoxal-5'-phosphate (PLP) in biological samples is challenging due to the presence of endogenous PLP in matrices used for preparation of calibrators and quality control samples (QCs). Hence, we have developed an LC-MS/MS method for accurate and precise measurement of the concentrations of PLP in samples (20μL) of human whole blood that addresses this issue by using a surrogate matrix and minimizing the matrix effect. We used a surrogate matrix comprising 2% bovine serum albumin (BSA) in phosphate buffer saline (PBS) for making calibrators, QCs and the concentrations were adjusted to include the endogenous PLP concentrations in the surrogate matrix according to the method of standard addition...
September 1, 2015: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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