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https://www.readbyqxmd.com/read/28806777/a-synthetic-sickness-screen-for-senescence-re-engagement-targets-in-mutant-cancer-backgrounds
#1
Claire J Cairney, Lauren S Godwin, Alan E Bilsland, Sharon Burns, Katrina H Stevenson, Lynn McGarry, John Revie, Jon D Moore, Ceri M Wiggins, Rebecca S Collinson, Clare Mudd, Elpida Tsonou, Mahito Sadaie, Dorothy C Bennett, Masashi Narita, Christopher J Torrance, W Nicol Keith
Senescence is a universal barrier to immortalisation and tumorigenesis. As such, interest in the use of senescence-induction in a therapeutic context has been gaining momentum in the past few years; however, senescence and immortalisation remain underserved areas for drug discovery owing to a lack of robust senescence inducing agents and an incomplete understanding of the signalling events underlying this complex process. In order to address this issue we undertook a large-scale morphological siRNA screen for inducers of senescence phenotypes in the human melanoma cell line A375P...
August 14, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28806752/the-escrt-iii-pathway-facilitates-cardiomyocyte-release-of-cbin1-containing-microparticles
#2
Bing Xu, Ying Fu, Yan Liu, Sosse Agvanian, Robert C Wirka, Rachel Baum, Kang Zhou, Robin M Shaw, TingTing Hong
Microparticles (MPs) are cell-cell communication vesicles derived from the cell surface plasma membrane, although they are not known to originate from cardiac ventricular muscle. In ventricular cardiomyocytes, the membrane deformation protein cardiac bridging integrator 1 (cBIN1 or BIN1+13+17) creates transverse-tubule (t-tubule) membrane microfolds, which facilitate ion channel trafficking and modulate local ionic concentrations. The microfold- generated microdomains continuously reorganize, adapting in response to stress to modulate the calcium signaling apparatus...
August 14, 2017: PLoS Biology
https://www.readbyqxmd.com/read/28806702/hmgb1-redox-during-sepsis
#3
Wasan Abdulmahdi, Devika Patel, May M Rabadi, Tala Azar, Edson Jules, Mark Lipphardt, Rameen Hashemiyoon, Brian B Ratliff
During sepsis, the alarmin HMGB1 is released from tissues and promotes systemic inflammation that results in multi-organ damage, with the kidney particularly susceptible to injury. The severity of inflammation and pro-damage signaling mediated by HMGB1 appears to be dependent on the alarmin's redox state. Therefore, we examined HMGB1 redox in kidney cells during sepsis. Using intravital microscopy, CellROX labeling of kidneys in live mice indicated increased ROS generation in the kidney perivascular endothelium and tubules during lipopolysaccharide (LPS)-induced sepsis...
August 4, 2017: Redox Biology
https://www.readbyqxmd.com/read/28806700/a-large-raman-scattering-cross-section-molecular-embedded-sers-aptasensor-for-ultrasensitive-aflatoxin-b1-detection-using-cs-fe3o4-for-signal-enrichment
#4
Quansheng Chen, Mingxiu Yang, Xiaojing Yang, Huanhuan Li, Zhiming Guo, M H Rahma
With growing concern on oil safety problems, developing a simple and sensitive method to detect Aflatoxin B1 (AFB1), a common mycotoxin in peanut oil, is very necessary. In this study, Surface-enhanced Raman Scattering (SERS) aptasensor was developed for ultrasensitive AFB1 detection using the amino-terminal AFB1 aptamer (NH2-DNA1); and thiol-terminal AFB1 complementary aptamer (SH-DNA2) conjugated magnetic-beads (CS-Fe3O4) as enrichment nanoprobe and AuNR@DNTB@Ag nanorods (ADANRs) as reporter nanoprobe respectively...
August 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28806670/transmission-surface-plasmon-resonance-techniques-and-their-potential-biosensor-applications
#5
REVIEW
Chutiparn Lertvachirapaiboon, Akira Baba, Sanong Ekgasit, Kazunari Shinbo, Keizo Kato, Futao Kaneko
Transmission surface plasmon resonance (TSPR) is an unusual extraordinary optical transmission that is more transparent at certain wavelengths than expected by classical theory. The three main plasmonic structures that providing this phenomenon are nanohole arrays, diffraction gratings, and nanoslit arrays. This extraordinary optical transmission phenomenon is produced as a result of surface plasmon excitations. The shifting in TSPR responses upon changing of dielectric environment at the surface of a metallic film was observed...
August 1, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28806656/sensitive-surface-enhanced-raman-scattering-of-tio2-ag-nanowires-induced-by-photogenerated-charge-transfer
#6
Xin Zhao, Weiwei Zhang, Chuchu Peng, Yujie Liang, Wenzhong Wang
In this work, the semiconductor TiO2 nanowires were successfully coupled with plasmonic metal Ag nanoparticles to fabricate hybrid nanostructures with enhanced sensitive Raman substrate. The SERS activities of fabricated hybrid nanostructures were evaluated by detecting the Raman signals of R6G molecules. The fabricated TiO2/Ag nanowire/nanoparticle hybrid nanostructures show sensitive detection ability for R6G molecules. Based on the band structures of Ag nanoparticle, TiO2 nanowire and R6G molecule, the enhanced sensitive SERS activities of hybrid nanostructures is ascribed to an efficient charge transfer process, in which the photogenerated electrons transfer to the conduction band of TiO2 nanowires from metal Ag nanoparticles, and then to LUMO level of R6G molecules, leading to enhanced SERS activities...
August 8, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28806534/knockdown-of-snail-inhibits-epithelial-mesenchymal-transition-of-human-laryngeal-squamous-cell-carcinoma-hep-2-cells-through-vdr-signaling-pathway
#7
Xue Zhao, Dan Yu, Jingpu Yang, Kai Xue, Yan Liu, Chunshun Jin
It has been well-documented that Snail plays a decisive role in various tumors. However, the direct effect of Snail on laryngeal squamous cell carcinoma (LSCC) has not been elaborated. In this study, we firstly detected the expression of Snail in 14 samples of patients with LSCC and found that its content was high in cancer tissues compared with adjacent tissues. Then we established LSCC Hep-2 cells with Snail silencing and validated the knockdown efficiency by western blotting and real-time PCR. Results showed that silencing of Snail significantly inhibited the ability of adhesion, migration, and invasion of Hep-2 cells...
August 14, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28806446/sema3a-reduces-sprouting-of-adult-rod-photoreceptors-in-vitro
#8
Frank Kung, Weiwei Wang, Tracy S Tran, Ellen Townes-Anderson
Purpose: Rod photoreceptor terminals respond to retinal injury with retraction and sprouting. Since the guidance cue Semaphorin3A (Sema3A) is observed in the retina after injury, we asked whether Sema3A contributes to structural plasticity in rod photoreceptors. Methods: We used Western blots and alkaline phosphatase (AP)-tagged neuropilin-1 (NPN-1) to detect the expression of Sema3A in an organotypic model of porcine retinal detachment. We then examined Sema3A binding to cultured salamander rod photoreceptors using AP-tagged Sema3A...
August 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28806067/immunosensor-employing-stable-solid-1-amino-2-naphthyl-phosphate-and-ammonia-borane-toward-ultrasensitive-and-simple-point-of-care-testing
#9
Jeongwook Seo, Hyeri Ha, Seonhwa Park, Al-Monsur Jiaul Haque, Sinyoung Kim, Jung Min Joo, Haesik Yang
Biosensors for ultrasensitive point-of-care testing require dried reagents with long-term stability and a high signal-to-background ratio. Although ortho-substituted diaromatic dihydroxy and aminohydroxy compounds undergo fast redox reactions, they are not used as electrochemical signaling species because they are readily oxidized and polymerized by dissolved oxygen. In this report, stable, solid 1-amino-2-naphthyl phosphate (1A2N-P) and ammonia-borane (H3N-BH3) are respectively employed as a substrate for alkaline phosphatase (ALP) and a reductant for electrochemical-chemical (EC) redox cycling...
August 14, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28806064/conditional-displacement-hybridization-assay-for-multiple-snp-phasing
#10
Tsz Wing Fan, Henson L Lee Yu, I-Ming Hsing
The two chromosomal copies of the human genome are highly polymorphic, and the allelic content on each strand can dictate a person's biological outcomes. While many of the current diagnostic tools are able to detect the presence of multiple mutations at the same time, most cannot determine the phase of these mutations unless long-range PCR or sequencing techniques are used or if templates are compartmentalized into single copies prior to amplification. Here, an enzyme-coupled hybridization assay, named Conditional Displacement Hybridization Assay (CDHA), is described for the concurrent and rapid determination of the presence and phase of SNP variants...
August 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28806060/a-smart-dna-machine-for-carcinoembryonic-antigen-detection-by-exonuclease-iii-assisted-target-recycling-and-dna-walker-cascade-amplification
#11
Mengqi He, Kun Wang, Wen-Jing Wang, Yong-Liang Yu, Jian-Hua Wang
Synthetic DNA machine performs quasi-mechanical movements in response to external intervention, suggesting the promise of constructing sensitive and specific biosensors. Herein, a smart DNA walker biosensor for label-free detection of carcinoembryonic antigen (CEA) is developed for the first time by a novel cascade amplification strategy of exonuclease (Exo) III-assisted target recycling (ERA) and DNA walker. ERA as the first stage of amplification generates the walker-DNA, while the autonomous traveling of the walker-DNA on the substrate-modified silica microspheres as the second stage of amplification produces an ultrasensitive fluorescent signal with the help of N-methylmesoporphyrin IX (NMM)...
August 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28805807/notch-signaling-pathway-and-gene-expression-profiles-during-early-in-vitro-differentiation-of-liver-derived-mesenchymal-stromal-cells-to-osteoblasts
#12
Ksymena Urbanek, Marta Lesiak, Daniel Krakowian, Halina Koryciak-Komarska, Wirginia Likus, Piotr Czekaj, Damian Kusz, Aleksander L Sieroń
Notch signaling is a key signaling pathway for cell proliferation and differentiation. Therefore, we formulated a working hypothesis that Notch signaling can be used to detect early osteoblastic differentiation of mesenchymal stromal cells. Changes in expression and distribution of Notch 1, 2, 3, and Delta1 in the cytoplasm and nuclei of rat liver-derived mesenchymal stromal cells differentiating into osteoblasts were investigated, together with the displacement of intracellular domains (ICDs) of the receptors...
August 14, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28805751/characterization-of-quadratic-nonlinearity-between-motion-artifact-and-acceleration-data-and-its-application-to-heartbeat-rate-estimation
#13
Sunho Kim, Sungbin Im, Taehyung Park
Accelerometers are applied to various applications to collect information about movements of other sensors deployed at diverse fields ranging from underwater area to human body. In this study, we try to characterize the nonlinear relationship between motion artifact and acceleration data. The cross bicoherence test and the Volterra filter are used as the approaches to detection and modeling. We use the cross bicoherence test to directly detect in the frequency domain and we indirectly identify the nonlinear relationship by improving the performance of eliminating motion artifact in heartbeat rate estimation using a nonlinear filter, the second-order Volterra filter...
August 14, 2017: Sensors
https://www.readbyqxmd.com/read/28805692/real-time-rain-rate-evaluation-via-satellite-downlink-signal-attenuation-measurement
#14
Filippo Giannetti, Ruggero Reggiannini, Marco Moretti, Elisa Adirosi, Luca Baldini, Luca Facheris, Andrea Antonini, Samantha Melani, Giacomo Bacci, Antonio Petrolino, Attilio Vaccaro
We present the NEFOCAST project (named by the contraction of "Nefele", which is the Italian spelling for the mythological cloud nymph Nephele, and "forecast"), funded by the Tuscany Region, about the feasibility of a system for the detection and monitoring of precipitation fields over the regional territory based on the use of a widespread network of new-generation Eutelsat "SmartLNB" (smart low-noise block converter) domestic terminals. Though primarily intended for interactive satellite services, these devices can also be used as weather sensors, as they have the capability of measuring the rain-induced attenuation incurred by the downlink signal and relaying it on an auxiliary return channel...
August 12, 2017: Sensors
https://www.readbyqxmd.com/read/28805606/detection-of-sea-type-%C3%AE-thalassemia-in-embryo-biopsies-by-digital-pcr
#15
Ta-Hsien Lee, Ya-Chiung Hsu, Chia Lin Chang
OBJECTIVE: Accurate and efficient pre-implantation genetic diagnosis (PGD) based on the analysis of single or oligo-cells is needed for timely identification of embryos that are affected by deleterious genetic traits in in vitro fertilization (IVF) clinics. Polymerase chain reaction (PCR) is the backbone of modern genetic diagnoses, and a spectrum of PCR-based techniques have been used to detect various thalassemia mutations in prenatal diagnosis (PND) and PGD. Among thalassemias, SEA-type α-thalassemia is the most common variety found in Asia, and can lead to Bart's hydrops fetalis and serious maternal complications...
August 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28805489/atorvastatin-attenuates-experimental-contrast-induced-acute-kidney-injury-a-role-for-tlr4-myd88-signaling-pathway
#16
Rongzheng Yue, Chuan Zuo, Jing Zeng, Baihai Su, Ye Tao, Songmin Huang, Rui Zeng
OBJECTIVES: To investigate the protective effect of different atorvastatin doses on contrast-induced acute kidney injury and the related mechanism. METHODS: Healthy male Sprague-Dawley (SD) rats were randomly divided into the blank control group, experimental control group and different-dose atorvastatin groups. A rat model of contrast-induced acute kidney injury was established. We detected changes in serum creatinine (Scr) and blood urea nitrogen (BUN) before and after model establishment, observed and scored renal tubular injury, analyzed rat renal cell apoptosis, and measure the expression of signal pathway proteins and downstream inflammatory factors...
November 2017: Renal Failure
https://www.readbyqxmd.com/read/28805443/the-effect-of-the-proportion-of-mismatching-trials-and-task-orientation-on-the-confidence-accuracy-relationship-in-unfamiliar-face-matching
#17
Rachel G Stephens, Carolyn Semmler, James D Sauer
Unfamiliar, one-to-one face matching has been shown to be error-prone. However, it is unknown whether there is a strong relationship between confidence and accuracy in this task. If there is, then confidence could be used as an indicator of accuracy in real-world face matching settings such as border security, where the objectively correct decision is typically unknown. Two experiments examined the overall confidence-accuracy relationship, as well as the relationship for positive (match) and negative (mismatch) decisions...
August 14, 2017: Journal of Experimental Psychology. Applied
https://www.readbyqxmd.com/read/28805339/what-happens-in-the-pith-stays-in-the-pith-tissue-localized-defense-responses-facilitate-chemical-niche-differentiation-between-two-spatially-separated-herbivores
#18
Gisuk Lee, Youngsung Joo, Sang-Gyu Kim, Ian T Baldwin
Herbivore attack is known to elicit systemic defense responses that spread throughout the hostplant and influence the performance of other herbivores. While these plant-mediated indirect competitive interactions are well-described, and the co-existence of herbivores from different feeding guilds is common, the mechanisms of co-existence are poorly understood. In both field and glasshouse experiments with a native tobacco, Nicotiana attenuata, we found no evidence of negative interactions when plants were simultaneously attacked by two spatially separated herbivores: a leaf chewer Manduca sexta and a stem borer Trichobaris mucorea...
August 14, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28805061/a-triple-helix-molecular-switch-electrochemical-ratiometric-biosensor-for-ultrasensitive-detection-of-nucleic-acids
#19
Erhu Xiong, Zhenzhen Li, Xiaohua Zhang, Jiawan Zhou, Xiaoxia Yan, Yunqing Liu, Jinhua Chen
Biomolecular receptors such as nucleic acids that switch between two or more conformations upon binding to a specific target can be used to build specific and sensitive biosensors. In this work, based on the electrochemical dual-signaling ratiometric strategy and triple-helix molecular switch, we developed a selective, reusable, and simple electrochemical DNA (E-DNA) biosensor for target DNA (T-DNA) detection. A hairpin DNA capture probe labeled with methylene blue (MB-DNA) self-assembles on the surface of gold electrode (GE) through Au-S bond, and then a single-strand DNA modified with two ferrocenes (Fc-DNA) on each end to enhance the oxidation signal hybridizes with the MB-DNA to form a triple-helix conformation...
August 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28804779/language-mapping-using-t2-prepared-bold-functional-mri-in-the-presence-of-large-susceptibility-artifacts-initial-results-in-patients-with-brain-tumor-and-epilepsy
#20
Jun Hua, Xinyuan Miao, Shruti Agarwal, Chetan Bettegowda, Alfredo Quiñones-Hinojosa, John Laterra, Peter C M Van Zijl, James J Pekar, Jay J Pillai
At present, presurgical functional mapping is the most prevalent clinical application of functional magnetic resonance imaging (fMRI). Signal dropouts and distortions caused by susceptibility effects in the current standard echo planar imaging (EPI)-based fMRI images are well-known problems and pose a major hurdle for the application of fMRI in several brain regions, many of which are related to language mapping in presurgical planning. Such artifacts are particularly problematic in patients with previous surgical resection cavities, craniotomy hardware, hemorrhage, and vascular malformation...
June 2017: Tomography: a Journal for Imaging Research
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