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https://www.readbyqxmd.com/read/29778001/patchy-gold-coated-fe-3-o-4-nanospheres-with-enhanced-catalytic-activity-applied-for-paper-based-bipolar-electrode-electrochemiluminescence-aptasensors
#1
Xin Zhang, Ning Bao, Xiliang Luo, Shou-Nian Ding
In this work, novel multifunctional patchy gold coated Fe3 O4 hybrid nanoparticles (PG-Fe3 O4 NPs) have been successfully synthesized in aqueous medium via a facile adsorption-reduction method. A rational formation mechanism has been proposed by monitoring the morphological evolution. The PG-Fe3 O4 NPs retained the good magnetic property and exhibited excellent catalytical effeciency towards the electrochemical reduction of hydrogen peroxide. Chronoamperometric and amperometric experiments indicated a relatively high catalytic rate constant of 3...
May 10, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29777816/aptamer-based-assay-for-monitoring-genetic-disorder-phenylketonuria-pku
#2
Mohammad Hasanzadeh, Amir Zargami, Hossein Navay Baghban, Ahad Mokhtarzadeh, Nasrin Shadjou, Soltanali Mahboob
The genetic disorder phenylketonuria (PKU) is the inability to metabolize phenylalanine because of a lack of the enzyme phenylalanine hydroxylase. Phenylalanine is used to biochemically form proteins, coded for by DNA. The development of an apta-assay for detection of l-Phenylalanine is presented in this work. A highly specific DNA-aptamer, selected to l-Phenylalanine was immobilized onto a gold nanostructure and electrochemical measurements were performed in a solution containing the phosphate buffer solution with physiological pH...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29777784/cd146-mediates-an-e-cadherin-to-n-cadherin-switch-during-tgf-%C3%AE-signaling-induced-epithelial-mesenchymal-transition
#3
Yanbin Ma, Haofeng Zhang, Chaoliang Xiong, Zheng Liu, Qingji Xu, Jing Feng, Jun Zhang, Zhaoqing Wang, Xiyun Yan
Cadherin switch is an initiating factor of epithelial-mesenchymal transition (EMT) and is intimately correlated with cancer metastatic potential; however, its underlying mechanisms remain unclear. Here, using a transforming growth factor-β (TGF-β)-induced EMT model, we provide explicit evidence that CD146, with elevated expression and activity in a variety of cancers, is a key factor involved in the cadherin switch. We show that CD146 can be induced by TGF-β signaling. Moreover, CD146 expression is positively correlated with the activation levels of STAT3/Twist and ERK pathways...
May 16, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29777776/distinct-roles-for-the-cell-adhesion-molecule-contactin2-in-the-development-and-function-of-neural-circuits-in-zebrafish
#4
Suman Gurung, Emilia Asante, Devynn Hummel, Ashley Williams, Oren Feldman-Schultz, Mary C Halloran, Vinoth Sittaramane, Anand Chandrasekhar
Contactin2 (Cntn2)/Transient Axonal Glycoprotein 1 (Tag1), a neural cell adhesion molecule, has established roles in neuronal migration and axon fasciculation in chick and mouse. In zebrafish, antisense morpholino-based studies have indicated roles for cntn2 in the migration of facial branchiomotor (FBM) neurons, the guidance of the axons of the nucleus of the medial longitudinal fascicle (nucMLF), and the outgrowth of Rohon-Beard (RB) central axons. To study functions of Cntn2 in later stages of neuronal development, we generated cntn2 mutant zebrafish using CRISPR-Cas9...
May 16, 2018: Mechanisms of Development
https://www.readbyqxmd.com/read/29777762/astrocyte-elevated-gene-1-is-a-novel-regulator-of-astrogliosis-and-excitatory-amino-acid-transporter-2-via-interplaying-with-nuclear-factor-%C3%AE%C2%BAb-signaling-in-astrocytes-from-amyotrophic-lateral-sclerosis-mouse-model-with-hsod1-g93a-mutation
#5
Xiang Yin, Shuyu Wang, Yan Qi, Xudong Wang, Hongquan Jiang, Tianhang Wang, Yueqing Yang, Ying Wang, Chunting Zhang, Honglin Feng
AEG-1 has received extensive attention on cancer research. However, little is known about its roles in astrogliosis of Amyotrophic lateral sclerosis (ALS). In this study, we detected AEG-1 expression in hSOD1G93A -positive (mut-SOD1) astrocytes and wild type (wt-SOD1) astrocytes, and intend to elucidate its potential functions in ALS related astrogliosis and the always accompanied dysregulated glutamate clearance. Results showed elevated protein and mRNA levels of AEG-1 in mut-SOD1 astrocytes; Also, NF-κB signaling pathway related proteins and inflammatory cytokines were upregulated in mut-SOD1 astrocytes; AEG-1 knockdown attenuated astrocytes proliferation and pro-inflammatory release; also we found that AEG-1 silence inhibited translocation of p65 from cytoplasma to nuclear, which was associated with inhibited NF-κB signaling...
May 16, 2018: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/29777731/tumor-necrosis-factor-receptor-2-is-required-for-ischemic-preconditioning-mediated-neuroprotection-in-the-hippocampus-following-a-subsequent-longer-transient-cerebral-ischemia
#6
Jae-Chul Lee, Chan Woo Park, Myoung Cheol Shin, Jun Hwi Cho, Hyang-Ah Lee, Young-Myeong Kim, Joon Ha Park, Ji Hyeon Ahn, Jeong Hwi Cho, Hyun-Jin Tae, In Koo Hwang, Tae-Kyeong Lee, Moo-Ho Won, Il Jun Kang
Tumor Necrosis Factor-α (TNF-α) is a proinflammatory cytokine implicated in neuronal damage in response to cerebral ischemia. Ischemic preconditioning (IPC) provides neuroprotection against a subsequent severer or longer transient ischemia by ischemic tolerance. Here, we focused on the role of TNF-α in IPC-mediated neuroprotection against neuronal death following a subsequent longer transient cerebral ischemia (TCI). Gerbils used in this study were randomly assigned to eight groups; sham group, TCI operated group, IPC plus (+) sham group, IPC + TCI operated group, sham + etanercept (an inhibitor of TNF-a) group, TCI + etanercept group, IPC + sham + etanercept group, and IPC + TCI + etanercept group...
May 16, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29777700/transient-receptor-potential-ankyrin-1-channels-are-involved-in-spontaneous-peptide-hormone-release-from-astrocytes
#7
Mai Takizawa, Kazuki Harada, Kazuaki Nakamura, Takashi Tsuboi
Astrocytes, a large population of glial cells, detect neurotransmitters and respond by increasing intracellular Ca2+ concentration ([Ca2+ ]i ) and releasing chemical molecules called gliotransmitters. Recently discovered Ca2+ influx through transient receptor potential ankyrin 1 (TRPA1) channels is reported to cause spontaneous [Ca2+ ]i increase in astrocytes. While several physiological functions of TRPA1-mediated spontaneous Ca2+ signal have been revealed, relation with gliotransmitter release, especially peptide hormone exocytosis is largely unknown...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777679/development-of-dnazyme-based-pcr-signal-cascade-amplification-for-visual-detection-of-listeria-monocytogenes-in-food
#8
Zhanmin Liu, Chenhui Yao, Cuiyun Yang, Yanming Wang, Sibao Wan, Junyi Huang
Listeria monocytogenes is an important food-borne pathogen, and it can cause severe diseases. Rapid detection of L. monocytogenes is crucial to control this pathogen. A simple and robust strategy based on the cascade of PCR and G-quadruplex DNAzyme catalyzed reaction was used to detect L. monocytogenes. In the presence of hemin and the aptamer formed during PCR, the catalytic horseradish peroxidase-mimicking G-quadruplex DNAzymes allow the colorimetric responses of target DNA from L. monocytogenes. This assay can detect genomic DNA of L...
May 16, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29777674/an-approach-for-brain-controlled-prostheses-based-on-scene-graph-steady-state-visual-evoked-potentials
#9
Rui Li, Xiaodong Zhang, Hanzhe Li, Liming Zhang, Zhufeng Lu, Jiangcheng Chen
Brain control technology can restore communication between the brain and a prosthesis, and choosing a Brain-Computer Interface (BCI) paradigm to evoke electroencephalogram (EEG) signals is an essential step for developing this technology. In this paper, the Scene Graph paradigm used for controlling prostheses was proposed; this paradigm is based on Steady-State Visual Evoked Potentials (SSVEPs) regarding the Scene Graph of a subject's intention. A mathematic model was built to predict SSVEPs evoked by the proposed paradigm and a sinusoidal stimulation method was used to present the Scene Graph stimulus to elicit SSVEPs from subjects...
May 16, 2018: Brain Research
https://www.readbyqxmd.com/read/29777599/immense-random-colocalization-revealed-by-automated-high-content-image-cytometry-seriously-questions-fish-as-gold-standard-for-detecting-eml4-alk-fusion
#10
Gábor Smuk, Tamás Tornóczky, László Pajor, Ilse Chudoba, Béla Kajtár, Veronika Sárosi, Gábor Pajor
EML4-ALK gene fusion (inv2(p21p23)) of non-small cell lung cancer (NSCLC) predisposes to tyrosine kinase inhibitor treatment. One of the gold standard diagnostics is the dual color (DC) break-apart (BA) FISH technique, however, the unusual closeness of the involved genes has been suggested to raise likelihood of random co-localization (RCL) of signals. Although this is suspected to decrease sensitivity (often to as low as 40-70%), the exact level and effect of RCL has not been revealed thus far. Signal distances were analyzed to the 0...
May 19, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29777586/platelets-autotaxin-and-lysophosphatidic-acid-signaling-win-win-factors-for-cancer-metastasis
#11
REVIEW
Raphael Leblanc, Audrey Houssin, Olivier Peyruchaud
Platelets play a crucial role in the survival of metastatic cells in the blood circulation. Interaction of tumor cells with platelets leads to the production of plethoric factors among which our review will focus on lysophosphatidic acid (LPA) because platelets are the highest producers of this bioactive lysophospholipid in the organism. LPA promotes platelet aggregation and blocking platelet function leads to inhibition of breast cancer cell metastasis through decreased LPA signaling. Autotaxin (ATX), a lysophospholipase D responsible for basal concentration of LPA in blood, was detected in platelet α-granules...
May 19, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29777445/a-reverse-genetics-system-for-enterovirus-d68-using-human-rna-polymerase-i
#12
Minglei Pan, Shuai Gao, Zhenwei Zhou, Keke Zhang, Sihua Liu, Zhiyun Wang, Tao Wang
Human enterovirus D68 (EV-D68) is a highly contagious virus, which causes respiratory tract infections. However, no effective vaccines are currently available for controlling EV-D68 infection. Here, we developed a reverse genetics system to recover EV-D68 minireplicons and infectious EV-D68 from transfected plasmids using the RNA polymerase I (Pol I) promoter. The EV-D68 minireplicons contained the luciferase reporter gene, which flanked by the non-coding regions of the EV-D68 RNA. The luciferase signals could be detected in cells after transfection and Pol I promoter-mediated luciferase signal was significantly stronger than that mediated by the T7 promoter...
May 17, 2018: Virus Genes
https://www.readbyqxmd.com/read/29777330/down-regulating-il-6-gp130-targets-improved-the-anti-tumor-effects-of-5-fluorouracil-in-colon-cancer
#13
Sanhong Li, Jilai Tian, Hongming Zhang, Shoubing Zhou, Xiyong Wang, Lei Zhang, Jiapeng Yang, Zhigang Zhang, Zhenling Ji
Recent studies have confirmed that IL-6/GP130 targets are closely associated with tumor growth, metastasis and drug resistance. 5-Fluorouracil (5-FU) is the most common chemotherapeutic agent for colon cancer but is limited due to chemoresistance and high cytotoxicity. Bazedoxifene (BZA), a third-generation selective estrogen receptor modulator, was discovered by multiple ligand simultaneous docking and drug repositioning approaches to have a novel function as an IL-6/GP130 target inhibitor. Thus, we speculated that in colon cancer, the anti-tumor efficacy of 5-FU might be increased in combination with IL-6/GP130 inhibitors...
May 18, 2018: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/29777313/gold-nanocluster-based-ratiometric-fluorescent-probes-for-hydrogen-peroxide-and-enzymatic-sensing-of-uric-acid
#14
Dongqin Yang, Minchuan Luo, Junwei Di, Yifeng Tu, Jilin Yan
A method is described for ratiometric fluorometric assays of H2 O2  by using two probes that have distinct response profiles. Under the catalytic action of ferrous ion, the 615 nm emission of protein-stabilized gold nanoclusters (under 365 nm photoexcitation) is quenched by H2 O2 , while an increased signal is generated with a peak at 450 nm by oxidizing coumarin with the H2 O2 /Fe(II) system to form a blue emitting fluorophore. These decrease/increase responses give a ratiometric signal. The ratio of the fluorescences at the two peaks are linearly related to the concentration of H2 O2 in the range from 0...
May 18, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29777309/fluorometric-determination-of-vibrio-parahaemolyticus-using-an-f-0-f-1-atpase-based-aptamer-and-labeled-chromatophores
#15
Nuo Duan, Shijia Wu, Huiling Zhang, Ying Zou, Zhouping Wang
An F0 F1 -ATPase-based aptasensor is described for the fluorometric determination of Vibrio parahaemolyticus. Chromatophores containing F0 F1 -ATPases were first prepared from Rhodospirillum rubrum cells. Then, an aptamer-functionalized chromatophore acts as the capture probe, and a chromatophore labeled with the pH probe fluorescein acts as the signalling probe. In the presence of V. parahaemolyticus, the rotation rate of F0 F1 -ATPase is decreased due to the formation of the aptamer-chromatophore complex...
May 18, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29777008/multispectral-fluorescence-imaging-as-a-tool-to-separate-healthy-and-disease-related-lymphatic-anatomies-during-robot-assisted-laparoscopic-procedures
#16
Philippa Meershoek, Gijs H KleinJan, Matthias N van Oosterom, Esther M Wit, Danny M van Willigen, Kevin P Bauwens, Erik J van Gennep, Alexandre M Mottrie, Henk G van der Poel, Fijs van Leeuwen
To reduce the invasive nature of extended pelvic lymph node dissections (ePLND) in prostate cancer, we have developed a multicolor fluorescence guidance approach that enables the discrimination between prostate draining lymph nodes (LNs) and lower limb draining LNs. Methods: In five porcine models multispectral-fluorescence guidance was performed using da Vinci Si- and Xi-robot consoles. They received fluorescein into the lower limb(s) and indocyanine green (ICG)-nanocolloid into the prostate. Results: Fluorescein was detected in 29 LNs (average of 3...
May 18, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29776952/activity-based-concept-to-screen-biological-matrices-for-opiates-and-synthetic-opioids
#17
Annelies Cannaert, Lakshmi Vasudevan, Melissa Friscia, Amanda L A Mohr, Sarah M R Wille, Christophe P Stove
BACKGROUND: Detection of new highly potent synthetic opioids is challenging as new compounds enter the market. Here we present a novel screening method for the detection of opiates and (synthetic) opioids based on their activity. METHODS: A cell-based system was set up in which activation of the μ -opioid receptor (MOR) led to recruitment of β-arrestin 2, resulting in functional complementation of a split NanoLuc luciferase and allowing readout via bioluminescence...
May 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29776597/the-role-of-fdg-pet-in-detecting-rejection-after-liver-transplantation
#18
Ashley M Watson, Neal Bhutiani, Prejesh Philips, Eric G Davis, Mary Eng, Robert M Cannon, Christopher M Jones
BACKGROUND: The activation and increased metabolic activity of T cells in acute cellular rejection could allow fluoro-2-deoxyglucose positron emission tomography to be utilized for detection of acute cellular rejection. The objective of this study was to evaluate the effectiveness of fluoro-2-deoxyglucose positron emission tomography in detecting acute cellular rejection in the clinical setting. METHODS: Fluoro-2-deoxyglucose positron emission tomography studies were performed on 88 orthotopic liver transplant patients at 7 and 17 days postoperatively (first positron emission tomography and second positron emission tomography, respectively)...
May 15, 2018: Surgery
https://www.readbyqxmd.com/read/29776446/valproic-acid-attenuates-traumatic-spinal-cord-injury-induced-inflammation-via-stat1-and-nf-%C3%AE%C2%BAb-pathway-dependent-of-hdac3
#19
Shoubo Chen, Jingfang Ye, Xiangrong Chen, Jinnan Shi, Wenhua Wu, Wenping Lin, Weibin Lin, Yasong Li, Huangde Fu, Shun Li
BACKGROUND: Microglial polarization with M1/M2 phenotype shifts and the subsequent neuroinflammatory responses are vital contributing factors for spinal cord injury (SCI)-induced secondary injury. Nuclear factor-κB (NF-κB) is considered the central transcription factor of inflammatory mediators, which plays a crucial role in microglial activation. Lysine acetylation of STAT1 seems necessary for NF-kB pathway activity, as it is regulated by histone deacetylases (HDACs). There have been no studies that have explained if HDAC inhibition by valproic acid (VPA) affects the NF-κB pathway via acetylation of STAT1 dependent of HDAC activity in the microglia-mediated central inflammation following SCI...
May 18, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29776333/transcriptomic-analysis-of-genes-in-soybean-in-response-to-peronospora-manshurica-infection
#20
Hang Dong, Shuangfeng Shi, Chong Zhang, Sihui Zhu, Mei Li, Jie Tan, Yue Yu, Liping Lin, Shirong Jia, Xujing Wang, Yuanhua Wu, Yuhui Liu
BACKGROUND: Soybean downy mildew (SDM), caused by Peronospora manshurica (Pm), is a major fungal disease in soybean. To date, little is known regarding the defense mechanism at molecular level and how soybean plants response to Pm infection. In this study, differential gene expression in SDM-resistant (HR) and SDM-susceptible (HS) genotype was analyzed by RNA-seq to identify differentially expressed genes (DEGs) following Pm infection. RESULTS: Of a total of 55,017 genes mapped to the soybean reference genome sequences, 2581 DEGs were identified...
May 18, 2018: BMC Genomics
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