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https://www.readbyqxmd.com/read/28917607/selection-of-reliable-reference-genes-for-gene-expression-studies-in-botrytis-cinerea
#1
He Ren, Xiaoqing Wu, Yuping Lyu, Hongzi Zhou, Xueying Xie, Xinjian Zhang, Hetong Yang
Botrytis cinerea is an important plant pathogen causing grey mold disease in a wide range of plant species. The aim of this study was to identify reliable reference genes that can be used for the analysis of relative gene expression in B. cinerea with quantitative real-time reverse transcription PCR (qRT-PCR). Six commonly used housekeeping genes including actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ubiquitin (UBQ), ubiquitin-conjugating enzyme (UCE), α-tubulin (α-TUB) and β-tubulin (β-TUB) were selected to test their expression stabilities in B...
September 13, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28916765/radiation-damage-at-the-active-site-of-human-alanine-glyoxylate-aminotransferase-reveals-that-the-cofactor-position-is-finely-tuned-during-catalysis
#2
Giorgio Giardina, Alessandro Paiardini, Riccardo Montioli, Barbara Cellini, Carla Borri Voltattorni, Francesca Cutruzzolà
The alanine:glyoxylate aminotransferase (AGT), a hepatocyte-specific pyridoxal-5'-phosphate (PLP) dependent enzyme, transaminates L-alanine and glyoxylate to glycine and pyruvate, thus detoxifying glyoxylate and preventing pathological oxalate precipitation in tissues. In the widely accepted catalytic mechanism of the aminotransferase family, the lysine binding to PLP acts as a catalyst in the stepwise 1,3-proton transfer, interconverting the external aldimine to ketimine. This step requires protonation by a conserved aspartate of the pyridine nitrogen of PLP to enhance its ability to stabilize the carbanionic intermediate...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28912866/compositional-analysis-of-various-layers-of-upper-urinary-tract-stones-by-infrared-spectroscopy
#3
Zhang He, Zhang Jing, Zheng Jing-Cun, Hu Chuan-Yi, Gao Fei
The objective of the present study was to determine the composition of various layers of upper urinary stones and assess the mechanisms of stone nucleation and aggregation. A total of 40 integrated urinary tract stones with a diameter of >0.8 cm were removed from the patients. All of the stones were cut in half perpendicularly to the longitudinal axis. Samples were selected from nuclear, internal and external layers of each stone. Fourier transform infrared spectroscopy (FT-IR) was adopted for qualitative and quantitative analysis of all of the fragments and compositional differences among nuclear, internal and external layers of various types of stone were subsequently investigated...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28906061/opposite-effect-of-polymorphic-mutations-on-the-electrostatic-aggregation-of-human-alanine-glyoxylate-aminotransferase-implications-for-the-pathogenesis-of-primary-hyperoxaluria-type-i
#4
Mirco Dindo, Carolina Conter, Barbara Cellini
Protein aggregates formation is the basis of several misfolding diseases, including those displaying loss-of-function pathogenesis. Although aggregation is often attributed to the population of intermediates exposing hydrophobic surfaces, the contribution of electrostatic forces has recently gained attention. Here we combined computational and in vitro studies to investigate the aggregation process of human peroxisomal alanine:glyoxylate aminotransferase (AGT), a pyridoxal 5'-phosphate (PLP)-dependent enzyme involved in glyoxylate detoxification...
September 14, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28905951/order-disorder-phase-transition-in-an-anhydrous-pyrazole-based-proton-conductor-the-enhancement-of-electrical-transport-properties
#5
M Widelicka, K Pogorzelec-Glaser, A Pietraszko, P Ławniczak, R Pankiewicz, A Łapiński
The crystal structure of 1H-pyrazol-2-ium hydrogen oxalate has been studied at 100 K. It consists of two-dimensional layers built with one-dimensional chains that contain pyrazolium and oxalate acids bonded by N-HO and O-HO hydrogen bonds. According to the X-ray data and the Quantum Theory of Atoms in Molecules, it was shown that weak and moderate hydrogen bonds are present in the crystal at room temperature. The thermal stability was studied with the DSC, TGA, and DTG methods: three endothermic peaks are observed at 384, 420, and 469 K...
September 14, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28895601/n-n-diethyl-diaminopropane-copper-ii-oxalate-self-reducible-complex-for-the-solution-based-synthesis-of-copper-nanocrystals
#6
Takanari Togashi, Masato Nakayama, Ryosuke Miyake, Keirei Uruma, Katsuhiko Kanaizuka, Masato Kurihara
Metal oxalates (C2O4(2-), ox) have been explored as promising precursors for the direct transformation of their oxalate moieties into metallic or metal oxide crystals via thermal decomposition without the formation of any byproducts due to releasing CO2 gas. The copper(ii) oxalate (Cu(ox)) crystal is a coordination polymer composed of an infinite coordination network with a thermal decomposition temperature around 300 °C; however, their insoluble nature in any solvents and relatively high decomposition temperature do not allow the solution-based syntheses of surface-modified metallic Cu nanocrystals (NCs) in the presence of various surfactants such as long-chain alkylamines and alkylcarboxylates which have been used for increasing the dispersibility of NCs in organic solvents...
September 12, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28894252/bof-steel-slag-as-a-low-cost-sorbent-for-vanadium-v-removal-from-soil-washing-effluent
#7
Yuchen Gao, Jianguo Jiang, Sicong Tian, Kaimin Li, Feng Yan, Nuo Liu, Meng Yang, Xuejing Chen
Soil washing is an effective remediation method to remove heavy metals from contaminated soil. However, it produces wastewater that contains large amounts of heavy metals, which lead to serious pollution. This study investigated the removal of vanadium (V) from synthetic soil washing effluent using BOF steel slag. The effects of particle size, slag dosage, initial pH, and initial vanadium concentration on removal behavior were studied. Adsorption kinetics and isotherms were also analyzed. The results showed that the vanadium removal efficiency increased as the steel slag particle size decreased and as the amount of slag increased...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28894241/an-enhanced-electrochemical-and-cycling-properties-of-novel-boronic-ionic-liquid-based-ternary-gel-polymer-electrolytes-for-rechargeable-li-licoo2-cells
#8
K Karuppasamy, Hyun-Seok Kim, Dongkyu Kim, Dhanasekaran Vikraman, K Prasanna, A Kathalingam, Ramakant Sharma, Hee Woo Rhee
A new generation of boronic ionic liquid namely 1-ethyl-3-methylimidazolium difluoro(oxalate)borate (EMImDFOB) was synthesized by metathesis reaction between 1-ethyl-3-methylimiazolium bromide and lithium difluoro(oxalate)borate (LiDFOB). Ternary gel polymer electrolyte membranes were prepared using electrolyte mixture EMImDFOB/LiDFOB with poly vinylidenefluoride-co-hexafluoropropylene (PVdF-co-HFP) as a host matrix by facile solvent-casting method and plausibly demonstrated its feasibility to use in lithium ion batteries...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28892797/study-of-the-intensification-of-solar-photo-fenton-degradation-of-carbamazepine-with-ferrioxalate-complexes-and-ultrasound
#9
A J Expósito, J M Monteagudo, A Durán, I San Martín, L González
The intensification of the solar photo-Fenton system with ferrioxalate photoactive complexes and ultrasound applied to the mineralization of 15mg/L carbamazepine aqueous solution (CBZ) was evaluated. The experiments were carried out in a solar compound parabolic collector (CPC) pilot plant reactor coupled to an ultrasonic processor. The dynamic behavior of hydroxyl radicals generated under the different studied reaction systems was discussed. The initial concentrations of hydrogen peroxide and ferrous/oxalic acid and pH were found to be the most significant variables (32...
August 30, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28891390/pathology-and-epidemiology-of-oxalate-nephrosis-in-cheetahs
#10
Emily P Mitchell, Molly E Church, Sarah M Nemser, Betsy Jean Yakes, Eric R Evans, Renate Reimschuessel, Karin Lemberger, Peter N Thompson, Karen A Terio
To investigate cases of acute oxalate nephrosis without evidence of ethylene glycol exposure, archived data and tissues from cheetahs ( Acinonyx jubatus) from North America ( n = 297), southern Africa ( n = 257), and France ( n = 40) were evaluated. Renal and gastrointestinal tract lesions were characterized in a subset of animals with ( n = 100) and without ( n = 165) oxalate crystals at death. Crystals were confirmed as calcium oxalate by Raman spectroscopy in 45 of 47 cheetahs tested. Crystals were present in cheetahs from 3...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28891172/urolithiasis-is-prevalent-and-associated-with-reduced-bone-mineral-density-in-%C3%AE-thalassaemia-major
#11
Phillip Wong, Frances Milat, Peter J Fuller, Peter G Kerr, James C G Doery, Danielle H Oh, Dana Jackson, Matthew T Gillespie, Donald K Bowden, Sant-Rayn Pasricha, Ken K Lau
Asymptomatic urolithiasis is common and of mixed composition in patients with β-thalassaemia major. Twenty-seven subjects were imaged using dual-energy computer tomography to determine the presence and composition of urolithiasis. The prevalence of urolithiasis was 59% and affected patients generally had multiple stones, often with more than one component: struvite (33%), calcium oxalate (31%) and cystine (22%). Hypercalciuria was present in 78% of subjects and calcium-containing urolithiasis was associated with reduced femoral neck Z scores...
September 2017: Internal Medicine Journal
https://www.readbyqxmd.com/read/28886706/can-randall-s-plug-composed-of-calcium-oxalate-form-via-the-free-particle-mechanism
#12
F Grases, O Söhnel
BACKGROUND: The likelihood of a Randall's plug composed of calcium oxalate monohydrate (COM) forming by the free particle mechanism in a model of kidney with a structure recently described by Robertson was examined at the most favourable conditions for the considered mechanism. METHODS: The Robertson model of the kidney is used in the following development. The classical theory of crystallization was used for calculations. RESULTS: Initial COM nuclei were assumed to form at the beginning of the ascending loop of Henle where the supersaturation with respect to COM has been shown to reach the threshold level for spontaneous nucleation...
September 8, 2017: BMC Urology
https://www.readbyqxmd.com/read/28882031/a-multiregional-italian-cohort-of-24-hour-urine-metabolic-evaluation-in-renal-stone-formers
#13
Francesco Esperto, Martino Marangella, Alberto Trinchieri, Michele Petrarulo, Roberto Miano
BACKGROUND: Nephrolithiasis is a common condition with several studies documenting an increased prevalence over the past four decades. EAU and AUA guidelines recommend 24-hour urine metabolic evaluation in high-risk stone formers. Aim of this study is to retrospectively evaluate the first three years of experience with Lithotest® (Biohealth Italia Srl, Turin, Italy) through the analysis of demographic, clinical and biochemical data collected from a large cohort of patients with kidney stones...
September 7, 2017: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/28878507/improvement-of-yield-and-quality-of-two-spinacia-oleracea-l-varieties-by-using-different-fertilizing-approaches
#14
Ola Alessa, Safaa Najla, Ramzi Murshed
The effects of different fertilizers [the control with no fertilizer (C), inorganic fertilization (I), combined inorganic and organic fertilizer (IOHumax1) and (IOHumax2)] on yield and nutrients contents of two spinach varieties ("Balady and Virofly") were investigated. Significant effects of variety were observed on vegetative growth and nutrients contents. While Virofly had significantly higher leaf area (236.96 cm(2)), stem diameter (7.43 mm) and fresh weight of vegetative and radical parts (15.05 and 0...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878341/a-botrytis-cinerea-klp-7-kinesin-acts-as-a-virulence-determinant-during-plant-infection
#15
Pamil Tayal, Sumit Raj, Esha Sharma, Manoj Kumar, Vikram Dayaman, Nidhi Verma, Abhimanyu Jogawat, Meenakshi Dua, Rupam Kapoor, Atul Kumar Johri
Botrytis cinerea is a necrotrophic pathogen that infects many important crops. In an attempt to unravel some novel factors that govern pathogenicity in B. cinerea, Agrobacterium tumefaciens mediated transformation (ATMT) was deployed, and a number of tagged transformants were generated. Among these, a mutant, BCM-29 exhibited slower growth rate, reduced conidia size, conidiation and penetration. The mutant was also defective in secretion of oxalic acid (OA) and exhibited reduced activities of polygalacturonase (PG) and pectin methyl esterases (PME)...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28875258/molecular-analysis-of-oxalate-induced-endoplasmic-reticulum-stress-mediated-apoptosis-in-the-pathogenesis-of-kidney-stone-disease
#16
Albert Abhishek, Shaly Benita, Monika Kumari, Divya Ganesan, Eldho Paul, Ponnusamy Sasikumar, Ayyavu Mahesh, Subramani Yuvaraj, Tharmarajan Ramprasath, Govindan Sadasivam Selvam
Oxalate, a non-essential end product of metabolism, causes hyperoxaluria and eventually calcium oxalate (CaOx) stone disease. Kidney cells exposed to oxalate stress results in generation of reactive oxygen species (ROS) and progression of stone formation. Perturbations in endoplasmic reticulum (ER) result in accumulation of misfolded proteins and Ca(2+) ions homeostasis imbalance and serve as a common pathway for various diseases, including kidney disorders. ER stress induces up-regulation of pro-survival protein glucose-regulated protein 78 (GRP78) and pro-apoptotic signaling protein C/EBP homologous protein (CHOP)...
September 5, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28874210/ultrastructural-elemental-and-mineralogical-analysis-of-vascular-calcification-in-atherosclerosis
#17
Ida Perrotta, Edoardo Perri
Over the past few decades, remarkable progress has been achieved in terms of understanding the molecular and cellular mechanisms of atherosclerotic vascular calcification and the important role of matrix vesicles in initiating and propagating pathologic tissue mineralization has been widely recognized. Despite these recent advances, however, no definitive data are currently available regarding the texture and composition of the minerals that grow in the vessel wall during the course of the disease. Using different electron microscopy imaging and analysis, we demonstrate that vascular cells can produce and secrete more than one type of matrix vesicles which act as sites for initial mineral deposition independently of their structural features...
September 6, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/28872864/measurement-of-the-energy-and-high-pressure-dependence-of-x-ray-induced-decomposition-of-crystalline-strontium-oxalate
#18
David Goldberger, Egor Evlyukhin, Petrika Cifligu, Yonggang Wang, Michael Pravica
We report measurements of the X-ray induced decomposition of crystalline strontium oxalate (SrC2O4) as function of energy and high-pressure in two experiments. SrC2O4 at ambient conditions was irradiated with monochromatic synchrotron X-rays ranging in energy from 15 to 28 keV. A broad resonance of the decomposition yield was observed with a clear maximum when irradiating with 20 keV X-rays and ambient pressure. Little or no decomposition was observed at 15 keV which is below the Sr K-shell energy of 16.12 keV suggesting that excitation of K electrons may play an important role in the destabilization of the C2O42- anion...
September 5, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28871300/polymer-immobilized-hybrid-multi-catalyst-architecture-for-enhanced-electrochemical-oxidation-of-glycerol
#19
Florika C Macazo, David P Hickey, Sofiene Abdellaoui, Matthew S Sigman, Shelley D Minteer
The development of a hybrid, tri-catalytic architecture is demonstrated by immobilizing MWCNTs, TEMPO-modified linear poly(ethylenimine) and oxalate decarboxylase on an electrode to enable enhanced electrochemical oxidation of glycerol. This immobilized, hybrid catalytic motif results in a synergistic 3.3-fold enhancement of glycerol oxidation and collects up to 14 electrons per molecule of glycerol.
September 14, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28867090/nutrients-and-natural-toxic-substances-in-commonly-consumed-jerusalem-artichoke-helianthus-tuberosus-l-tuber
#20
Kunchit Judprasong, Nidthida Archeepsudcharit, Kedsiri Chantapiriyapoon, Pharrunrat Tanaviyutpakdee, Piya Temviriyanukul
This study determined nutrients, chemical contaminants, (insecticide residues and heavy metals), and natural toxic substances (nitrate, nitrite, cyanide, oxalate, phytate, and trypsin inhibitor) in tubers of Jerusalem artichokes-Kaentawan in the Thai language-grown in four major provinces in Thailand. They were purchased, prepared, homogenized, and freeze-dried for further analysis using standard methods. All Kaentawan samples contained considerable amounts of fructans and dietary fiber (15.4±0.2gand3.2±0...
January 1, 2018: Food Chemistry
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