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https://www.readbyqxmd.com/read/28435474/the-vision-of-industrie-4-0-in-the-making-a-case-of-future-told-tamed-and-traded
#1
Sabine Pfeiffer
Since industrial trade fair Hannover Messe 2011, the term "Industrie 4.0" has ignited a vision of a new Industrial Revolution and has been inspiring a lively, ongoing debate among the German public about the future of work, and hence society, ever since. The discourse around this vision of the future eventually spread to other countries, with public awareness reaching a temporary peak in 2016 when the World Economic Forum's meeting in Davos was held with the motto "Mastering the Fourth Industrial Revolution...
2017: Nanoethics
https://www.readbyqxmd.com/read/28425289/imaging-metastable-states-and-transitions-in-proteins-by-trajectory-map
#2
Chuanbiao Zhang, Jin Yu, Xin Zhou
It has been a long-standing and intriguing issue to develop robust methods to identify metastable states and interstate transitions from simulations or experimental data in order to understand the functional conformational changes of proteins. Most of the existing methods are usually hard to define the complicated boundaries of the states in the conformational space, and thus often lead to parameter-sensitive results. Here we present a new approach, named as visualized Trajectory Map (vTM), to identify the metastable states and the rare interstate transitions, by considering both the conformation similarity and the temporal-successiveness of conformations...
April 20, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28419507/a-rapid-solvent-accessible-surface-area-estimator-for-coarse-grained-molecular-simulations
#3
Shuai Wei, Charles L Brooks, Aaron T Frank
The rapid and accurate calculation of solvent accessible surface area (SASA) is extremely useful in the energetic analysis of biomolecules. For example, SASA models can be used to estimate the transfer free energy associated with biophysical processes, and when combined with coarse-grained simulations, can be particularly useful for accounting for solvation effects within the framework of implicit solvent models. In such cases, a fast and accurate, residue-wise SASA predictor is highly desirable. Here, we develop a predictive model that estimates SASAs based on Cα-only protein structures...
June 5, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28402469/health-system-functionality-in-a-low-income-country-in-the-midst-of-conflict-the-case-of-yemen
#4
Naseeb Qirbi, Sharif A Ismail
Background:  Although the literature on effects of armed conflict on population health is extensive, detailed assessments of effects on public health 'systems' are few. This article aims to help address this deficit through the medium of a case study on Yemen, describing health system and health outcome performance prior to the internationalisation of the conflict there in March 2015, before assessing the impact of war on health system functionality since that time. Method : Review of peer- and non-peer reviewed literature from 2005 to 2016 from academic sources, multilateral organizations, donors and governmental and non-governmental organizations, augmented by secondary data analysis...
April 11, 2017: Health Policy and Planning
https://www.readbyqxmd.com/read/28401474/tracking-effects-of-sil1-increase-taking-a-closer-look-beyond-the-consequences-of-elevated-expression-level
#5
Thomas Labisch, Stephan Buchkremer, Vietxuan Phan, Laxmikanth Kollipara, Christian Gatz, Chris Lentz, Kay Nolte, Jörg Vervoorts, José Andrés González Coraspe, Albert Sickmann, Stephanie Carr, René P Zahedi, Joachim Weis, Andreas Roos
SIL1 acts as a co-chaperone for the major ER-resident chaperone BiP and thus plays a role in many BiP-dependent cellular functions such as protein-folding control and unfolded protein response. Whereas the increase of BiP upon cellular stress conditions is a well-known phenomenon, elevation of SIL1 under stress conditions was thus far solely studied in yeast, and different studies indicated an adverse effect of SIL1 increase. This is seemingly in contrast with the beneficial effect of SIL1 increase in surviving neurons in neurodegenerative disorders such as amyotrophic lateral sclerosis and Alzheimer's disease...
April 11, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28397157/the-dynamics-of-de-adoption-a-case-study-of-policy-change-de-adoption-and-replacement-of-an-evidence-based-hiv-intervention
#6
Virginia R McKay, M Margaret Dolcini, Lee D Hoffer
Evidence-based intervention (EBI) de-adoption and its influence on public health organizations are largely unexplored within public health implementation research. However, a recent shift in support for HIV prevention EBIs by the Centers for Disease Control and Prevention provides an opportunity to explore EBI de-adoption. The current mixed-method study examines EBI de-adoption and the subsequent impact on a community-based organization (CBO) dedicated to HIV prevention. We conducted a case study with a CBO implementing RESPECT, an HIV prevention EBI, over 5 years (2010-2014), but then de-adopted the intervention...
April 10, 2017: Translational Behavioral Medicine
https://www.readbyqxmd.com/read/28390054/virtual-patients-in-a-behavioral-medicine-massive-open-online-course-mooc-a-qualitative-and-quantitative-analysis-of-participants-perceptions
#7
Anne H Berman, Gabriele Biguet, Natalia Stathakarou, Beata Westin-Hägglöf, Kerstin Jeding, Cormac McGrath, Nabil Zary, Andrzej A Kononowicz
OBJECTIVE: The purpose of this article is to explore learners' perceptions of using virtual patients in a behavioral medicine Massive Open Online Course (MOOCs) and thereby describe innovative ways of disseminating knowledge in health-related areas. METHODS: A 5-week MOOC on behavioral medicine was hosted on the edX platform. The authors developed two branched virtual patients consisting of video recordings of a live standardized patient, with multiple clinical decision points and narration unfolding depending on learners' choices...
April 7, 2017: Academic Psychiatry
https://www.readbyqxmd.com/read/28390017/interaction-of-tio2-nanoparticles-with-proteins-from-aquatic-organisms-the-case-of-gill-mucus-from-blue-mussel
#8
Adeline Bourgeault, Véronique Legros, Florence Gonnet, Regis Daniel, Aurélie Paquirissamy, Clémence Bénatar, Olivier Spalla, Corinne Chanéac, Jean-Philippe Renault, Serge Pin
To better understand the mechanisms of TiO2 nanoparticle (NP) uptake and toxicity in aquatic organisms, we investigated the interaction of NPs with the proteins found in gill mucus from blue mussels. Mucus is secreted by many aquatic organisms and is often their first line of defense against pathogens, xenobiotics, and other sources of environmental stress. Here, five TiO2 NPs and one SiO2 NP were incubated with gill mucus and run out on a one-dimensional polyacrylamide gel for a comparative qualitative analysis of the free proteins in the mucosal solution and the proteins bound to NPs...
April 7, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28360918/the-unfolded-protein-response-supports-plant-development-and-defense-as-well-as-responses-to-abiotic-stress
#9
REVIEW
Yan Bao, Stephen H Howell
The unfolded protein response (UPR) is a stress response conserved in eukaryotic organisms and activated by the accumulation of misfolded proteins in the endoplasmic reticulum (ER). Adverse environmental conditions disrupt protein folding in the ER and trigger the UPR. Recently, it was found that the UPR can be elicited in the course of plant development and defense. During vegetative plant development, the UPR is involved in normal root growth and development, the effect of which can be largely attributed to the influence of the UPR on plant hormone biology...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28346457/confined-space-facilitates-g-quadruplex-formation
#10
Prakash Shrestha, Sagun Jonchhe, Tomoko Emura, Kumi Hidaka, Masayuki Endo, Hiroshi Sugiyama, Hanbin Mao
Molecular simulations suggest that the stability of a folded macromolecule increases in a confined space due to entropic effects. However, due to the interactions between the confined molecular structure and the walls of the container, clear-cut experimental evidence for this prediction is lacking. Here, using DNA origami nanocages, we show the pure effect of confined space on the property of individual human telomeric DNA G-quadruplexes. We induce targeted mechanical unfolding of the G-quadruplex while leaving the nanocage unperturbed...
March 27, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28338903/disulfide-bridges-as-essential-elements-for-the-thermostability-of-lytic-polysaccharide-monooxygenase-lpmo10c-from-streptomyces-coelicolor
#11
Magali Tanghe, Barbara Danneels, Matthias Last, Koen Beerens, Ingeborg Stals, Tom Desmet
Lytic polysaccharide monooxygenases (LPMOs) are crucial components of cellulase mixtures but their stability has not yet been studied in detail, let alone been engineered for industrial applications. In this work, we have evaluated the importance of disulfide bridges for the thermodynamic stability of Streptomyces coelicolor LPMO10C. Interestingly, this enzyme was found to retain 34% of its activity after 2-h incubation at 80°C while its apparent melting temperature (Tm) is only 51°C. When its three disulfide bridges were broken, however, irreversible unfolding occurred and no residual activity could be detected after a similar heat treatment...
March 9, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28334996/amelogenesis-imperfecta-caused-by-n-terminal-enamelin-point-mutations-in-mice-and-men-is-driven-by-endoplasmic-reticulum-stress
#12
Steven J Brookes, Martin J Barron, Claire E L Smith, James A Poulter, Alan J Mighell, Chris F Inglehearn, Catriona J Brown, Helen Rodd, Jennifer Kirkham, Michael J Dixon
"Amelogenesis imperfecta" (AI) describes a group of inherited diseases of dental enamel that have major clinical impact. Here, we identify the aetiology driving AI in mice carrying a p.S55I mutation in enamelin; one of the most commonly mutated proteins underlying AI in humans. Our data indicate that the mutation inhibits the ameloblast secretory pathway leading to ER stress and an activated unfolded protein response (UPR). Initially, with the support of the UPR acting in pro-survival mode, Enamp.S55I heterozygous mice secreted structurally normal enamel...
March 11, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28329414/a-protocol-for-the-development-of-a-critical-thinking-assessment-tool-for-nurses-using-a-delphi-technique
#13
Elisabeth Jacob, Christine Duffield, Darren Jacob
AIM: The aim of this study is to develop an assessment tool to measure the critical thinking ability of nurses. BACKGROUND: As an increasing number of complex patients are admitted to hospitals, the importance of nurses recognising changes in health status and picking up on deterioration is more important. To detect early signs of complication requires critical thinking skills. Registered nurses are expected to commence their clinical careers with the necessary critical thinking skills to ensure safe nursing practice...
March 22, 2017: Journal of Advanced Nursing
https://www.readbyqxmd.com/read/28327745/dynamics-of-miura-patterned-foldable-sheets-in-shear-flow
#14
Sarit Dutta, Michael D Graham
We study the dynamics of piecewise rigid sheets containing predefined crease lines in shear flow. The crease lines act like hinge joints along which the sheet may fold rigidly, i.e. without bending any other crease line. We choose the crease lines such that they tessellate the sheet into a two-dimensional array of parallelograms. Specifically, we focus on a particular arrangement of crease lines known as a Miura-pattern in the origami community. When all the hinges are fully open the sheet is planar, whereas when all are closed the sheet folds over itself to form a compact flat structure...
March 22, 2017: Soft Matter
https://www.readbyqxmd.com/read/28327610/molecular-level-detection-and-localization-of-mechanical-damage-in-collagen-enabled-by-collagen-hybridizing-peptides
#15
Jared L Zitnay, Yang Li, Zhao Qin, Boi Hoa San, Baptiste Depalle, Shawn P Reese, Markus J Buehler, S Michael Yu, Jeffrey A Weiss
Mechanical injury to connective tissue causes changes in collagen structure and material behaviour, but the role and mechanisms of molecular damage have not been established. In the case of mechanical subfailure damage, no apparent macroscale damage can be detected, yet this damage initiates and potentiates in pathological processes. Here, we utilize collagen hybridizing peptide (CHP), which binds unfolded collagen by triple helix formation, to detect molecular level subfailure damage to collagen in mechanically stretched rat tail tendon fascicle...
March 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28327150/research-impact-of-systems-level-long-term-care-research-a-multiple-case-study
#16
Anita Kothari, Nedra Peter, Melissa Donskov, Tracy Luciani
BACKGROUND: Traditional reporting of research outcomes and impacts, which tends to focus on research product publications and grant success, does not capture the value, some contributions, or the complexity of research projects. The purpose of this study was to understand the contributions of five systems-level research projects as they were unfolding at the Bruyère Centre for Learning, Research and Innovation (CLRI) in long-term care (LTC) in Ottawa, Ontario, Canada. The research questions were, (1) How are partnerships with research end-users (policymakers, administrators and other public/private organisations) characterised? (2) How have interactions with the CLRI Management Committee and Steering Committee influenced the development of research products? (3) In what way have other activities, processes, unlinked actors or organisations been influenced by the research project activities? METHODS: The study was guided by Kok and Schuit's concept of research impacts, using a multiple case study design...
March 21, 2017: Health Research Policy and Systems
https://www.readbyqxmd.com/read/28317116/anatomical-segmentation-of-the-human-medial-prefrontal-cortex
#17
M Córcoles-Parada, N C J Müller, M Ubero, V M Serrano-Del-Pueblo, F Mansilla, P Marcos-Rabal, E Artacho-Pérula, M Dresler, R Insausti, G Fernández, M Muñoz-López
The medial prefrontal areas 32, 24, 14, and 25 (mPFC) form part of the limbic memory system, but little is known about their functional specialization in humans. To add anatomical precision to structural and functional magnetic resonance imaging (MRI) data, we aimed to identify these mPFC subareas in histological preparations of human brain tissue, determine sulci most consistently related with mPFC areal boundaries, and use these sulci to delineate mPFC areas in MRIs. To achieve this, we obtained three-dimensional MRI data from 11 ex vivo hemispheres and processed them for cyto- and myelo-architectonic analysis...
March 20, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28315699/the-neglected-functions-of-intrinsically-disordered-proteins-and-the-origin-of-life
#18
REVIEW
Laurent Jaeken
The example of gelatine shows that extended proteins behave quite differently than globular ones: with water they form a gel. Historically the colloid view of protoplasm was discredited in favour of membrane-(pump)-theory (MPT), but unjustified. In his association-induction hypothesis Ling demonstrates that MPT is full of contradictions and that the colloid view has to be re-considered. In that case IDP's play a crucial role in this. What Ling calls the 'living state' consists of the unitary protoplasmic structure from which it was experimentally demonstrated that it can survive and keep Na(+) and K(+) concentrations without a delineating membrane...
March 15, 2017: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/28300624/neurotoxicity-of-the-steroidal-alkaloids-tomatine-and-tomatidine-is-rip1-kinase-and-caspase-independent-and-involves-the-eif2%C3%AE-branch-of-the-endoplasmic-reticulum
#19
Daniela Correia da Silva, Paula B Andrade, Patrícia Valentão, David M Pereira
Steroidal alkaloids are a class of natural products that occur in several species of the Solanaceae family. In the case of the tomato plant (Lycopersicon esculentum Mill.), tomatine and its aglycone, tomatidine, are the most representative molecules. These steroidal alkaloids have already shown several potentially useful biological activities, from anticancer to anti-inflammatory or antibacterial. In this work, the toxicity of these molecules in neuronal cells, namely in the neuroblastoma cell line SH-SY5Y, was assessed, emphasis being given to the cellular mechanisms underlying the effects observed...
March 12, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28297603/improving-the-efficiency-of-non-equilibrium-sampling-in-the-aqueous-environment-via-implicit-solvent-simulations
#20
Hui Liu, Fu Chen, Huiyong Sun, Dan Li, Tingjun Hou
By means of estimators based on non-equilibrium work, equilibrium free energy differences or potentials of mean force (PMFs) of a system of interest can be computed from biased molecular dynamics (MD) simulations. The approach, however, is often plagued by slow conformational sampling and poor convergence, especially when the solvent effects are taken into account. Here, as a possible way to alleviate the problem, several widely used implicit-solvent models, which are derived from the analytic generalized Born (GB) equation and implemented in the AMBER suite of programs, were employed in free energy calculations based on non-equilibrium work and evaluated for their abilities to emulate explicit water...
March 23, 2017: Journal of Chemical Theory and Computation
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