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https://www.readbyqxmd.com/read/28433823/simultaneous-monitoring-of-intra-and-extracellular-nitric-oxide-in-living-cells-by-means-of-dual-color-fluorescence-imaging
#1
Hui-Wen Yao, Jian-Bo Chen, Xiao-Feng Guo, Hong Wang
A dual-color fluorescence imaging method for simultaneous monitoring of intra- and extracellular nitric oxide (NO) was developed. Assisted by confocal laser scanning microscope, the intra- and extracellular NO can be successfully visualized by using two selected probes, 4,4-difluoro-8-(3,4-diaminophenyl)-3,5-bis(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene (p-MOPB) and disodium 2,6-disulfonate-1,3-dimethyl-5-hexadecyl-8-(3,4-diaminophenyl)-4,4'-difluoro-4-bora-3a,4a-diaza-s-indacene (DSDMHDAB), which display distinct membrane permeability and show different colors of fluorescence after reaction with NO...
April 19, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28432000/determination-of-thiol-to-protein-ratio-and-drug-to-antibody-ratio-by-in-line-size-exclusion-chromatography-with-post-column-reaction
#2
Kenichiro Furuki, Toshimasa Toyo'oka
An in-line size-exclusion (SE) ultra-high-performance liquid chromatography (UHPLC)- 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB) method to quantify thiols in monoclonal antibodies (mAb) when manufacturing antibody-drug conjugates (ADCs) was developed. The mAbs are separated on an SE-UHPLC column and monitored with a UV detector at a wavelength of 280 nm. Eluents are channeled into a reaction coil and mixed with DTNB to form 5-thio-2-nitrobenzoic acid (TNB). Thiol concentration is calculated using absorption at 412 nm...
April 18, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28426220/fluorescent-neuraminidase-assay-based-on-supramolecular-dye-capture-after-enzymatic-cleavage
#3
Wenqi Liu, César F A Gómez-Durán, Bradley D Smith
A conceptually new type of enzymatic cleavage assay is reported that utilizes in-situ supramolecular capture of the fluorescent product. A squaraine-derived substrate with large blocking groups at each end of its structure cannot be threaded by a tetralactam macrocycle until the blocking groups are removed by enzyme cleavage. A prototype design responds to viral neuraminidase, an indicator of influenza infection, and also measures susceptibility of the sample to neuraminidase inhibitor drugs. The substrate structure incorporates three key features; (a) a bis(4-amino-3-hydroxyphenyl)squaraine core with bright deep-red fluorescence and excellent photostability, (b) an N-methyl group at each end of the squaraine core that ensures fast macrocycle threading kinetics, and (c) sialic acid blocking groups that prevent macrocycle threading until they are removed by viral neuraminidase...
April 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28415842/spectrophotometric-quantification-of-peroxidase-with-p-phenylene-diamine-for-analyzing-peroxidase-encapsulating-lipid-vesicles
#4
Ya Zhang, Yannick R F Schmid, Sandra Luginbühl, Qiang Wang, Petra S Dittrich, Peter Walde
A spectrophotometric assay for the determination of horseradish peroxidase (HRP) in aqueous solution with p-phenylenediamine (PPD, benzene-1,4-diamine) as electron donor substrate and hydrogen peroxide (H2O2) as oxidant was developed. The oxidation of PPD by HRP/H2O2 leads to the formation of Bandrowski's base ((3E,6E)-3,6-bis[(4-aminophenyl)imino]cyclohexa-1,4-diene-1,4-diamine), which can be quantified by following the increase in absorbance at 500 nm. The assay was applied for monitoring the activity of HRP inside ≈180 nm-sized lipid vesicles (liposomes), prepared from POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and purified by size exclusion chromatography...
April 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28414228/molecular-aspects-of-a-robust-assay-for-ferroxidase-function-of-ceruloplasmin
#5
Laura Cortes, Blaine R Roberts, Anthony G Wedd, Zhiguang Xiao
Ceruloplasmin (Cp) is one of the most complex multicopper oxidase enzymes and plays an essential role in the metabolism of iron in mammals. Ferrous ion supplied by the ferroportin exporter is converted by Cp to ferric ion that is accepted by plasma metallo-chaperone transferrin. Study of the enzyme at the atomic and molecular level has been hampered by the lack of a suitable ferrous substrate. We have developed the classic chromophoric complex Fe(II)Hx(Tar)2 (H2Tar, 4-(2-thiazolylazo)resorcinol; x = 0-2; overall charge omitted) as a robust substrate for evaluation of the ferroxidase function of Cp and related enzymes...
April 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28413239/tuning-the-si-n-interaction-in-metalated-oligosilanylsilatranes
#6
Mohammad Aghazadeh Meshgi, Rainer Zitz, Małgorzata Walewska, Judith Baumgartner, Christoph Marschner
Most known silatrane chemistry is concerned with examples where the attached silatrane substituent atom is that of an element more electronegative than silicon. The current study features silylated silatranes with a range of electropositive elements attached to the silyl group. The resulting compounds show different degrees of electron density on the silatrane-substituted silicon atom. This directly affects the Si-N interaction of the silatrane which can be monitored either by (29)Si NMR spectroscopy or directly by single crystal XRD analysis of the Si-N distance...
April 10, 2017: Organometallics
https://www.readbyqxmd.com/read/28410821/-analgesia-nociception-index-for-perioperative-analgesia-monitoring-in-spinal-surgery
#7
Guldem Turan, Arzu Yıldırım Ar, Yıldız Yigit Kuplay, Oznur Demiroluk, Mustafa Gazi, Nur Akgun, Erhan Celikoglu
BACKGROUND AND OBJECTIVES: The Analgesia Nociception Index is an index used to measure the levels of pain, sympathetic system activity and heart rate variability during general anesthesia. In our study, Analgesia Nociception Index monitoring in two groups who had undergone spinal stabilization surgery and were administered propofol-remifentanil (Total Intravenous Anesthesia) and sevoflurane-remifentanyl anesthesia was compared regarding its significance for prediction of postoperative early pain...
April 11, 2017: Revista Brasileira de Anestesiologia
https://www.readbyqxmd.com/read/28406989/escdl-1-a-new-cell-line-derived-from-chicken-embryonic-stem-cells-supports-efficient-replication-of-mardiviruses
#8
Jean-François Vautherot, Christian Jean, Laetitia Fragnet-Trapp, Sylvie Rémy, Danièle Chabanne-Vautherot, Guillaume Montillet, Aurélie Fuet, Caroline Denesvre, Bertrand Pain
Marek's disease virus is the etiological agent of a major lymphoproliferative disorder in poultry and the prototype of the Mardivirus genus. Primary avian somatic cells are currently used for virus replication and vaccine production, but they are largely refractory to any genetic modification compatible with the preservation of intact viral susceptibility. We explored the concept of induction of viral replication permissiveness in an established pluripotent chicken embryonic stem cell-line (cES) in order to derive a new fully susceptible cell-line...
2017: PloS One
https://www.readbyqxmd.com/read/28406618/microwave-mediated-synthesis-of-bulky-lanthanide-porphyrin-phthalocyanine-triple-deckers-electrochemical-and-magnetic-properties
#9
Hong-Guang Jin, Xiaoqin Jiang, Irina A Kühne, Sylvain Clair, Valérie Monnier, Christophe Chendo, Ghenadie Novitchi, Annie K Powell, Karl M Kadish, Teodor Silviu Balaban
Five heteroleptic lanthanide porphyrin-bis-phthalocyanine triple-decker complexes with bulky peripheral groups were prepared via microwave-assisted synthesis and characterized in terms of their spectroscopic, electrochemical, and magnetic properties. These compounds, which were easily obtained under our preparative conditions, would normally not be accessible in large quantities using conventional synthetic methods, as a result of the low yield resulting from steric congestion of bulky groups on the periphery of the phthalocyanine and porphyrin ligands...
April 13, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28391200/quasi-periodicities-detection-using-phase-rectified-signal-averaging-in-eeg-signals-as-a-depth-of-anesthesia-monitor
#10
Quan Liu, Yi-Feng Chen, Shou-Zen Fan, Maysam Abbod, Jiann-Shing Shieh
Phase-rectified signal averaging (PRSA) has been known to be a useful method to detect periodicities in non-stationary biological signals. Determination of quasi-periodicities in electroencephalogram (EEG) is a candidate for quantifying the changes of depth of anesthesia (DOA). In this paper, DOA monitoring capacity of periodicities detected using PRSA was quantified by assessing EEG signals collected from 56 patients during surgery. The method is compared to sample entropy (SampEn), detrended fluctuation analysis (DFA) and permutation entropy (PE)...
April 3, 2017: IEEE Transactions on Neural Systems and Rehabilitation Engineering
https://www.readbyqxmd.com/read/28375129/advanced-oxidation-of-acridine-orange-by-aqueous-alkaline-iodine
#11
Rafia Azmat, Noshab Qamar, Raheela Naz, Anum Khursheed
The advanced oxidation process is certainly used for the dye waste water treatment. In this continuation a new advanced oxidation via aqueous alkaline iodine was developed for the oxidation of acridine orange (AO) {3, 6 -bis (dimethylamino) acridine zinc chloride double salt}. Oxidation Kinetics of AO by alkaline solution of iodine was investigated spectrophotometrically at λmax 491 nm. The reaction was monitored at various operational parameters like several concentrations of dye and iodine, pH, salt electrolyte and temperature...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28371228/a-self-quenching-system-based-on-bis-naphthalimide-a-dual-two-photon-channel-gsh-fluorescent-probe
#12
Wei Shen, Jingyan Ge, Siyang He, Ruoyu Zhang, Chengyan Zhao, Yong Fan, Shian Yu, Bin Liu, Qing Zhu
A single fluorescent signal output often does not satisfy the detection accuracy requirements for bioactive compounds in biological systems. It is of great interest to develop a dual-channel turn-on fluorescence sensing system for self-validated detection. Herein, we report a self-quenching nanoparticle composed of dual two-photon dyes of naphthalimides, displaying almost no fluorescence at both channels, owing to aggregation-caused quenching (ACQ). A dual two-photon-channel fluorescent probe for GSH was further developed by inserting a disulfide bond between two naphthalimides...
April 1, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28362087/elucidating-the-structures-of-the-low-and-high-ph-mo-v-species-in-respiratory-nitrate-reductase-a-combined-epr-14-15-n-hyscore-and-dft-study
#13
Julia Rendon, Frédéric Biaso, Pierre Ceccaldi, René Toci, Farida Seduk, Axel Magalon, Bruno Guigliarelli, Stéphane Grimaldi
Respiratory nitrate reductases (Nars), members of the prokaryotic Mo/W-bis Pyranopterin Guanosine dinucleotide (Mo/W-bisPGD) enzyme superfamily, are key players in nitrate respiration, a major bioenergetic pathway widely used by microorganisms to cope with the absence of dioxygen. The two-electron reduction of nitrate to nitrite takes place at their active site, where the molybdenum ion cycles between Mo(VI) and Mo(IV) states via a Mo(V) intermediate. The active site shows two distinct pH-dependent Mo(V) electron paramagnetic resonance (EPR) signals whose structure and catalytic relevance have long been debated...
March 31, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28357586/behavioral-inhibition-system-sensitivity-enhances-motor-cortex-suppression-when-watching-fearful-body-expressions
#14
Sara Borgomaneri, Francesca Vitale, Alessio Avenanti
Influential theories suggest that a defensive behavioral inhibition system (BIS) supports the inhibition of action tendencies when facing potential threats. However, little is known about threat-related inhibitory mechanisms in humans and their relations to inter-individual differences in BIS sensitivity. To address this issue, we used paired-pulse TMS to investigate early human motor cortex (M1) responses to social signals of potential threats, like another's fearful body posture. In two experiments, participants observed pictures of fearful and happy postures, and neutral postures that were either dynamic (in Exp1) or static (in Exp2)...
March 29, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/28337908/detection-of-2-4-6-trinitrotoluene-using-a-miniaturized-disposable-electrochemical-sensor-with-an-ionic-liquid-gel-polymer-electrolyte-film
#15
Holly A Yu, Junqiao Lee, Simon W Lewis, Debbie S Silvester
A new electrochemical method to detect and quantify the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solutions is demonstrated. A disposable thin-film electrode modified with a droplet of a gel-polymer electrolyte (GPE) was immersed directly into samples of TNT at concentrations of 1-10 μg/mL. The GPE contained the hydrophobic room-temperature ionic liquid (RTIL) trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl)imide ([P14,6,6,6][NTf2]) and the polymer poly(hexyl methacrylate). The RTIL acted to preconcentrate TNT into the GPE and provided ionic conductivity...
April 4, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28328660/addressing-the-barriers-to-bioimpedance-spectroscopy-use-in-major-burns-alternate-electrode-placement
#16
Pippa Kenworthy, Tiffany L Grisbrook, Michael Phillips, William Gibson, Fiona M Wood, Dale W Edgar
Bioimpedance spectroscopy (BIS) is a method used to assess body composition and fluid distribution. As a technology for measurement of fluid shifts during acute burn resuscitation, there are potential barriers to its use due to the location of wounds. This study aimed to determine whether alternate electrode positions were a suitable alternative compared to standardized (manufacturer) positions in moderate to large size burns for the measurement of BIS resistance and fluid changes. BIS measurements were collected in standard and alternate electrode placements and in an open wound and Acticoat™ dressing condition...
March 15, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/28320979/exposure-to-4-4-methylene-bis-2-chloroaniline-mboca-in-new-south-wales-australia
#17
Kiran Shankar, Vivian Fung, Mahinda Seneviratne, Gregory E O'Donnell
OBJECTIVES: This study was conducted to determine the level of exposure of 4, 4'-methylene bis(2-chloroaniline) (MbOCA) in New South Wales (NSW), Australia. METHODS: An integrated occupational hygiene and biological monitoring program were used to assess the workers' exposure to MbOCA via inhalation, ingestion and dermal contact. This was conducted by personal air monitoring, static air monitoring and surface contamination monitoring of the work environment and biological monitoring of the workers' exposure to MbOCA at nine workplaces in NSW...
March 17, 2017: Journal of Occupational Health
https://www.readbyqxmd.com/read/28320196/extended-timed-up-and-go-assessment-as-a-clinical-indicator-of-cognitive-state-in-parkinson-s-disease
#18
Tess Evans, Alexa Jefferson, Michelle Byrnes, Sue Walters, Soumya Ghosh, Frank L Mastaglia, Brian Power, Ryan S Anderton
OBJECTIVE: To evaluate a modified extended Timed Up and Go (extended-TUG) assessment against a panel of validated clinical assessments, as an indicator of Parkinson's disease (PD) severity and cognitive impairment. METHODS: Eighty-seven participants with idiopathic PD were sequentially recruited from a Movement Disorders Clinic. An extended-TUG assessment was employed which required participants to stand from a seated position, walk in a straight line for 7m, turn 180° and then return to the start, in a seated position...
April 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28298244/frailty-related-to-anesthesia-guided-by-the-index-bispectral-frail-study-study-protocol-for-a-randomized-controlled-trial
#19
Morgan Le Guen, Marie Herr, Antoine Bizard, Caroline Bichon, Nathalie Boichut, Thierry Chazot, Ngai Liu, Joel Ankri, Marc Fischler
BACKGROUND: Currently, patients older than 60 years of age represent 25% of the population and are at an increased risk during surgery. Therefore, reducing postoperative morbidity and mortality is a major concern in medical research and practice. Dependence on caregivers and cognitive impairment represent two major risk factors in the elderly, especially in frail patients after surgery under general anesthesia. In this context, continuous monitoring of the depth of anesthesia using a bispectral index (BIS) sensor may reduce the occurrence of impairments by gaining better control of the anesthetic depth...
March 16, 2017: Trials
https://www.readbyqxmd.com/read/28275163/axonal-degeneration-in-retinal-ganglion-cells-is-associated-with-a-membrane-polarity-sensitive-redox-process
#20
Mohammadali Almasieh, Maria-Magdalena Catrinescu, Loïc Binan, Santiago Costantino, Leonard A Levin
Axonal degeneration is a pathophysiological mechanism common to several neurodegenerative diseases. The slow Wallerian degeneration (Wld(S)) mutation, which results in reduced axonal degeneration in the central and peripheral nervous systems, has provided insight into a redox-dependent mechanism by which axons undergo self-destruction. We studied early molecular events in axonal degeneration with single-axon laser axotomy and time-lapse imaging, monitoring the initial changes in transected axons of purified retinal ganglion cells (RGCs) from wild-type and Wld(S) rat retinas using a polarity-sensitive annexin-based biosensor (annexin B12-Cys101,Cys260-N,N'-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine)...
April 5, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
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