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https://www.readbyqxmd.com/read/28528133/chemical-synthesis-of-flower-like-hybrid-cu-oh-2-cuo-electrode-application-of-polyvinyl-alcohol-and-triton-x-100-to-enhance-supercapacitor-performance
#1
S K Shinde, V J Fulari, D-Y Kim, N C Maile, R R Koli, H D Dhaygude, G S Ghodake
In this research article, we report hybrid nanomaterials of copper hydroxide/copper oxide (Cu(OH)2/CuO). A thin films were prepared by using a facile and cost-effective successive ionic layer adsorption and reaction (SILAR) method. As-synthesized and hybrid Cu(OH)2/CuO with two different surfactants polyvinyl alcohol (PVA) and triton-X 100 (TRX-100) was prepared having distinct morphological, structural, and supercapacitor properties. The surface of the thin film samples were examined by scanning electron microscopy (SEM)...
May 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28512695/arsenic-induces-thioredoxin-1-and-apoptosis-in-human-liver-hhl-5-cells
#2
Yuanyuan Li, Yuanyuan Zhang, Yanhui Gao, Wei Zhang, Xiaohui Cui, Junqiu Liu, Yudan Wei
To further characterize the mechanisms underlying liver toxicity induced by arsenic, we examined in this study the effect of arsenic on thioredoxin (Trx) and the apoptotic signaling pathways in human liver HHL-5 cells. The cells were treated with 0, 2, 5, and 10 μM of sodium arsenite for 24 h, and the changes of Trx1 and thioredoxin reductase (TrxR1) as well as intracellular ROS and apoptosis were examined. A concentration-dependent increase in mRNA and protein levels of Trx1 and TrxR1 was observed in arsenic-treated cells...
May 17, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28505020/the-role-of-thioredoxin-1-in-suppression-sepsis-through-inhibiting-mitochondrial-induced-apoptosis-in-spleen
#3
Guobing Chen, Xiang Li, Mengbing Huang, Xiaoshuang Zhou, Ye Li, Xiaoqin Mao, Jie Bai
Sepsis is a serious public health issue and the leading cause of death in critically ill patients in intensive care units. Thioredoxin-1 (Trx-1) is a protein of regulating redox, as well as a modulator of inflammation and apoptosis. Our previous study reported that Trx-1 decreased endoplasmic reticulum-mediated inflammation involved in lung in a model of experimental sepsis. However, its effect on mitochondrial-mediated apoptosis in spleen has not been reported. We studied whether Trx-1 could prevent spleen cells apoptosis in sepsis...
June 2017: Shock
https://www.readbyqxmd.com/read/28500266/n-terminal-thioredoxin-reductase-domains-and-thioredoxins-act-concertedly-in-seedling-development
#4
Valle Ojeda, Juan M Pérez-Ruiz, María-Cruz González, Victoria A Nájera, Mariam Sahrawy, Antonio Jesús Serrato, Peter Geigenberger, Francisco J Cejudo
Thiol-dependent redox regulation of enzyme activity plays a central role in the rapid acclimation of chloroplast metabolism to ever fluctuating light availability. This regulatory mechanism relies on ferredoxin (Fdx) reduced by the photosynthetic electron transport chain, which fuels reducing power to thioredoxins (Trxs) via a Fdx-dependent Trx reductase (FTR). In addition, chloroplasts harbour an NADPH-dependent Trx reductase, which has a joint Trx domain at the C-terminus, termed NTRC. Thus, a relevant issue in chloroplast function is to establish the relationship between these two redox systems and its impact on plant development...
May 12, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28489026/redox-regulation-of-the-tumor-suppressor-pten-by-hydrogen-peroxide-and-tert-butyl-hydroperoxide
#5
Ying Zhang, Seong-Jeong Han, Iha Park, Inyoung Kim, Kee-Oh Chay, Seok Mo Kim, Dong Il Jang, Tae-Hoon Lee, Seung-Rock Lee
Organic peroxides and hydroperoxides are skin tumor promoters. Free radical derivatives from these compounds are presumed to be the prominent mediators of tumor promotion. However, the molecular targets of these species are unknown. Phosphatase and tensin homologs deleted on chromosome 10 (PTEN) are tumor suppressors that play important roles in cell growth, proliferation, and cell survival by negative regulation of phosphoinositol-3-kinase/protein kinase B signaling. PTEN is reversibly oxidized in various cells by exogenous and endogenous hydrogen peroxide...
May 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28483515/thioredoxin-1-promotes-colorectal-cancer-invasion-and-metastasis-through-crosstalk-with-s100p
#6
Feiyan Lin, Peili Zhang, Zhigui Zuo, Fule Wang, Ruichun Bi, Wenjing Shang, Aihua Wu, Ju Ye, Shaotang Li, Xuecheng Sun, Jianbo Wu, Lei Jiang
Thioredoxin-1 (Trx-1) is a small redox-regulating protein, which plays an important role in several cellular functions. Despite recent advances in understanding the biology of Trx-1, the role of Trx-1 and its underlying signaling mechanism in colorectal cancer (CRC) metastasis have not been extensively studied. In this study, we observed that Trx-1 expression is increased in CRC tissues compared to the paired non-cancerous tissues and is significantly correlated with clinical staging, lymph node metastasis and poor survival...
May 5, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28470597/harnessing-the-profinity-exact%C3%A2-system-for-expression-and-purification-of-heterologous-proteins-in-e-coli
#7
Yoav Peleg, Vadivel Prabahar, Dominika Bednarczyk, Tamar Unger
Highly purified recombinant proteins in large quantities are valuable material for biochemical and structural studies. To achieve this goal, versatile tools were developed to increase the expression of the recombinant proteins and to facilitate the purification process. Fusion tags are commonly used for enhancing expression and solubility and some can be used in the purification process. However, these tags may need to be removed by treatment with specific proteases in order to obtain the tag-free protein. The Profinity eXact™ system provides an alternative system for a fusion tag, enhancing expression and purification in one-step...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28461233/inhibition-of-reductase-systems-by-2-aapa-modulates-peroxiredoxin-oxidation-and-mitochondrial-function-in-a172-glioblastoma-cells
#8
Luiz Felipe de Souza, Ariana Ern Schmitz, Luana Caroline Schüler da Silva, Karen Andrinéia de Oliveira, Cláudia Beatriz Nedel, Carla Inês Tasca, Andreza Fabro de Bem, Marcelo Farina, Alcir Luiz Dafre
Thiol homeostasis has a critical role in the maintenance of proper cellular functions and survival, being coordinated by the action of several reductive enzymes, including glutathione (GSH)/glutathione reductase (GR) and thioredoxin (Trx)/thioredoxin reductase (TrxR) systems. Here, we investigated the effects of the GR inhibitor 2-acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylthiocarbonylamino)phenylthiocarbamoylsulfanyl]propionic acid (2-AAPA) on the activity of thiol reductases (GR and TrxR), redox balance and mitochondrial function of A172 glioblastoma cells...
April 29, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28461071/host-oxidative-folding-pathways-offer-novel-anti-chikungunya-virus-drug-targets-with-broad-spectrum-potential
#9
Rose M Langsjoen, Albert J Auguste, Shannan L Rossi, Christopher M Roundy, Heidy N Penate, Maria Kastis, Matthew K Schnizlein, Kevin C Le, Sherry L Haller, Rubing Chen, Stanley J Watowich, Scott C Weaver
Alphaviruses require conserved cysteine residues for proper folding and assembly of the E1 and E2 envelope glycoproteins, and likely depend on host protein disulfide isomerase-family enzymes (PDI) to aid in facilitating disulfide bond formation and isomerization in these proteins. Here, we show that in human HEK293 cells, commercially available inhibitors of PDI or modulators thereof (thioredoxin reductase, TRX-R; endoplasmic reticulum oxidoreductin-1, ERO-1) inhibit the replication of CHIKV chikungunya virus (CHIKV) in vitro in a dose-dependent manner...
April 28, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28447047/replacement-of-threonine-55-with-glycine-decreases-the-reduction-rate-of-ostrx20-by-glutathione
#10
Mitra Roodgar-Nashta, Azar Shahpiri
Thioredoxins (Trxs) are small ubiquitous oxidoreductase proteins with two redox-active Cys residues in a conserved active site (WCG/PPC) that regulate numerous target proteins via thiol/disulfide exchanges in the cells of prokaryotes and eukaryotes. The isoforms OsTrx23 with a typical active site (WCGPC) and OsTrx20 with an atypical active site (WCTPC) are two Trx h- type isoforms in rice that were previously found to be reduced by NADPH-dependent thioredoxin reductase and GSH/Grx system, respectively. In the present work the reduction of mutants G41TOsTrx23, T55GOsTrx20, K48DOsTrx20 and T55G-K48D OsTrx20 as well as wild types OsTrx23 and OsTrx20 were tested in the reaction containing either NADPH/NTR or glutathione (GSH)...
March 2017: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28445781/mitochondrial-ros-control-of-cancer
#11
REVIEW
María Del Pilar Sosa Idelchik, Ulrike Begley, Thomas J Begley, J Andrés Melendez
Mitochondria serves a primary role in energy maintenance but also function to govern levels of mitochondria-derived reactive oxygen species (mROS). ROS have long been established to play a critical role in tumorigenesis and are now considered to be integral to the regulation of diverse signaling networks that drive proliferation, tumor cell survival and malignant progression. mROS can damage DNA, activate oncogenes, block the function of tumor suppressors and drive migratory signaling. The mitochondrion's oxidant scavenging systems including SOD2, Grx2, GPrx, Trx and TrxR are key of the cellular redox tone...
April 23, 2017: Seminars in Cancer Biology
https://www.readbyqxmd.com/read/28444333/a-non-classical-member-of-the-protein-disulfide-isomerase-family-pdi7-of-arabidopsis-thaliana-localizes-to-the-cis-golgi-and-endoplasmic-reticulum-membranes
#12
Christen Y L Yuen, Pengfei Wang, Byung-Ho Kang, Kristie Matsumoto, David A Christopher
Members of the protein disulfide isomerase (PDI)-C subfamily are chimeric proteins containing the thioredoxin (Trx) domain of PDIs, and the conserved N- and C-terminal Pfam domains of Erv41p/Erv46p-type cargo receptors. They are unique to plants and chromalveolates. The Arabidopsis genome encodes three PDI-C isoforms: PDI7, PDI12, and PDI13. Here we demonstrate that PDI7 is a 65-kDa integral membrane glycoprotein expressed throughout many Arabidopsis tissues. Using a PDI7-specific antibody, we show through immunoelectron microscopy that PDI7 localizes to the endoplasmic reticulum (ER) and Golgi membranes in wild-type root tip cells, and was also detected in vesicles...
April 21, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28443683/redox-signaling-mediated-by-thioredoxin-and-glutathione-systems-in-the-central-nervous-system
#13
Xiaoyuan Ren, Lili Zou, Xu Zhang, Vasco Branco, Jun Wang, Cristina Carvalho, Arne Holmgren, Jun Lu
SIGNIFICANCE: The thioredoxin (Trx) and glutathione (GSH) systems play important roles in maintaining the redox balance in the brain, a tissue that is prone to oxidative stress due to its high-energy demand. These two disulfide reductase systems are active in various areas of the brain and are considered to be critical antioxidant systems in the central nervous system (CNS). Various neuronal disorders have been characterized to have imbalanced redox homeostasis. Recent Advances: In addition to their detrimental effects, recent studies have highlighted that reactive oxygen species/reactive nitrogen species (ROS/RNS) act as critical signaling molecules by modifying thiols in proteins...
May 18, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28442424/thioredoxin-is-not-a-marker-for-treatment-resistance-depression-but-associated-with-cognitive-function-an-rtms-study
#14
Efruz Pirdogan Aydın, Abdullah Genc, Mihriban Dalkıran, Ece Türkyilmaz Uyar, Ömer Akil Özer, Kayıhan Oğuz Karamustafalıoğlu
Elevated oxidative stress is known to play an important role in development of depression and cognitive dysfunction. To date, thioredoxin (TRX), an antioxidant protein, has been investigated as a marker for psychiatric disorders such as schizophrenia, bipolar disorder and autism but its relationship with depression is yet to be unknown. The aim of this study is to detect the TRX levels in patients with treatment-resistant depression (TRD), analyse the effect of rTMS (repetitive transcranial magnetic stimulation) application on TRX levels and display the relationship of TRX with cognitive areas...
April 22, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28427799/assessing-cumulative-acute-toxicity-of-chemoradiotherapy-in-head-and-neck-cancer-with-or-without-induction-chemotherapy
#15
Bhartesh A Shah, Muhammad M Qureshi, Jennifer M Logue, Timothy P Cooley, Ken S Zaner, Scharukh Jalisi, Minh Tam Truong
BACKGROUND: To compare cumulative acute toxicity in head and neck cancer patients treated with concurrent chemoradiotherapy alone (CCRT) versus induction chemotherapy (IC) followed by CCRT (I/CCRT). METHODS: 77 patients underwent definitive CCRT (30 I/CCRT and 47 CCRT). Toxicity was graded using the Common Terminology Criteria for Adverse Events version 4.0. Using the TAME adverse event reporting system, short-term toxicity (T) scores were generated for IC (TIC), CCRT (TCCRT), total treatment duration (TRx), post-treatment period (TPT) and an overall score (Toverall) from treatment start to post treatment period...
April 12, 2017: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/28423690/preimplantation-factor-pif-protects-cultured-embryos-against-oxidative-stress-relevance-for-recurrent-pregnancy-loss-rpl-therapy
#16
Lindsay F Goodale, Soren Hayrabedran, Krassimira Todorova, Roumen Roussev, Sivakumar Ramu, Christopher Stamatkin, Carolyn B Coulam, Eytan R Barnea, Robert O Gilbert
Recurrent pregnancy loss (RPL) affects 2-3% of couples. Despite a detailed work-up, the etiology is frequently undefined, leading to non-targeted therapy. Viable embryos and placentae express PreImplantation Factor (PIF). Maternal circulating PIF regulates systemic immunity and reduces circulating natural killer cells cytotoxicity in RPL patients. PIF promotes singly cultured embryos' development while anti-PIF antibody abrogates it. RPL serum induced embryo toxicity is negated by PIF. We report that PIF rescues delayed embryo development caused by <3 kDa RPL serum fraction likely by reducing reactive oxygen species (ROS)...
May 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28422068/a-low-cost-bluetooth-low-energy-transceiver-for-wireless-sensor-network-applications-with-a-front-end-receiver-matching-network-reusing-power-amplifier-load-inductor
#17
Zhen Liang, Bin Li, Mo Huang, Yanqi Zheng, Hui Ye, Ken Xu, Fangming Deng
In this work, a low cost Bluetooth Low Energy (BLE) transceiver for wireless sensor network (WSN) applications, with a receiver (RX)-matching network-reusing power amplifier (PA) load inductor, is presented. In order to decrease the die area, only two inductors were used in this work. Besides the one used in the voltage control oscillator (VCO), the PA load inductor was reused as the RX impedance matching component in the front-end. Proper controls have been applied to achieve high transmitter (TX) input impedance when the transceiver is in the receiving mode, and vice versa...
April 19, 2017: Sensors
https://www.readbyqxmd.com/read/28414055/dihydromyricetin-attenuated-ang-ii-induced-cardiac-fibroblasts-proliferation-related-to-inhibitory-of-oxidative-stress
#18
Qiuyi Song, Lulu Liu, Jin Yu, Jingyao Zhang, Mengting Xu, Linlin Sun, Huiqin Luo, Zhaosong Feng, Guoliang Meng
Dihydromyricetin (DMY) is one of the most important flavonoids in vine tea, which showed several pharmacological effects. However, information about the potential role of DMY on angiotensin II (Ang II) induced cardiac fibroblasts proliferation remains unknown. In the present study, cardiac fibroblasts isolated from neonatal Sprague-Dawley rats were pretreated with different concentrations of DMY (0-320μM) for 4h, or DMY (80μM) for different time (0-24h), followed by Ang II (100nM) stimulation for 24h, Then number of cardiac fibroblasts and content of hydroxyproline was measured...
April 13, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28411237/physical-interaction-between-human-ribonucleotide-reductase-large-subunit-and-thioredoxin-increases-colorectal-cancer-malignancy
#19
Meng Lou, Qian Liu, Guoping Ren, Jiling Zeng, Xueping Xiang, Yongfeng Ding, Qinghui Lin, Tingting Zhong, Xia Liu, Lijun Zhu, Hongyan Qi, Jing Shen, Haoran Li, Jimin Shao
Ribonucleotide reductase (RR) is the rate-limiting enzyme in DNA synthesis by catalyzing the reduction of ribonucleotides to deoxyribonucleotides. During each enzymatic turnover, reduction of the active site disulfide in the catalytic large subunit is performed by a pair of shuttle cysteine residues in its C-terminal tail. Thioredoxin (Trx) and Glutaredoxin (Grx) are ubiquitous redox proteins, catalyzing thiol-disulfide exchange reactions. Here, immunohistochemical examination of clinical colorectal cancer (CRC) specimens revealed that human thioredoxin1 (hTrx1), but not human glutaredoxin1 (hGrx1), was upregulated along with human RR large subunit (RRM1) in cancer tissues, and the expression levels of both proteins were correlated with cancer malignancy stage...
April 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28410843/left-ventricular-hypertrophy-does-not-prevent-heart-failure-in-experimental-hypertension
#20
H Hernán Gómez Llambí, G Cao, M Donato, D Suárez, G Ottaviano, A Müller, B Buchholz, R Gelpi, M Otero-Losada, J Milei
BACKGROUND: Left ventricular hypertrophy (LVH) secondary to hypertension has been accepted to prevent heart failure (HF) while paradoxically increasing cardiovascular morbi-mortality. OBJECTIVES: To evaluate whether antihypertensive treatment inhibits LVH, restores beta-adrenergic response and affects myocardial oxidative metabolism. METHODS: Ninety spontaneously hypertensive rats (SHR) were distributed into groups and treated (mg/kg, p.o...
March 27, 2017: International Journal of Cardiology
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