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https://www.readbyqxmd.com/read/28322443/all-trans-retinoic-acid-ameliorates-hepatic-stellate-cell-activation-via-suppression-of-thioredoxin-interacting-protein-expression
#1
Hiroki Shimizu, Toshiaki Tsubota, Keita Kanki, Goshi Shiota
Activation of hepatic stellate cells (HSCs) is the effector factor of hepatic fibrosis and hepatocellular carcinoma (HCC) development. Accumulating evidence suggests that retinoic acids (RAs), derivatives of vitamin A, contribute to prevention of liver fibrosis and carcinogenesis, however, regulatory mechanisms of RAs still remain exclusive. To elucidate RA signaling pathway, we previously performed a genome-wide screening of RA-responsive genes by in silico analysis of RA-response elements, and identified 26 RA-responsive genes...
March 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28296345/a-cleavable-self-assembling-tag-strategy-for-preparing-proteins-and-peptides-with-an-authentic-n-terminus
#2
Qing Zhao, Bihong Zhou, Xianxing Gao, Lei Xing, Xu Wang, Zhanglin Lin
Recombinant protein expression and purification remains a central need for biotechnology. Herein, we report a streamlined protein and peptide purification strategy using short self-assembling peptides and a C-terminal cleavage intein. In this strategy, the fusion protein is first expressed as an aggregate induced by the self-assembling peptide. Upon simple separation, the target protein or peptide with an authentic N-terminus is then released in the solution by intein-mediated cleavage. Different combinations of four self-assembling peptides (ELK16, L6 KD, FK and FR) with three inteins (Sce VMA, Mtu ΔI-CM and Ssp DnaB) were explored...
March 15, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28289905/expression-profile-of-desiccation-tolerance-factors-in-intertidal-seaweed-species-during-the-tidal-cycle
#3
Camila Fierro, Camilo López-Cristoffanini, Andrés Meynard, Carlos Lovazzano, Francisco Castañeda, Eduardo Guajardo, Loretto Contreras-Porcia
The transcriptional modulation of desiccation tolerance factors in P. orbicularis explains its successful recuperation after water deficit. Differential responses to air exposure clarify seaweed distribution along intertidal rocky zones. Desiccation-tolerant seaweed species, such as Pyropia orbicularis, can tolerate near 96% water loss during air exposure. To understand the phenotypic plasticity of P. orbicularis to desiccation, several tolerance factors were assessed by RT-qPCR, Western-blot analysis, and enzymatic assays during the natural desiccation-rehydration cycle...
March 13, 2017: Planta
https://www.readbyqxmd.com/read/28281242/regulation-of-a-kinase-anchoring-protein-12-by-heat-shock-protein-a12b-to-prevent-ventricular-dysfunction-following-acute-myocardial-infarction-in-diabetic-rats
#4
Vaithinathan Selvaraju, Sumanth C Suresh, Mahesh Thirunavukkarasu, Jayakanthan Mannu, Jocelyn L C Foye, Premendu P Mathur, J Alexander Palesty, Juan A Sanchez, David W McFadden, Nilanjana Maulik
We examined the effects of overexpressing HSPA12B on angiogenesis and myocardial function by intramyocardial administration of adenovirus encoding HSPA12B (Ad. HSPA12B) in a streptozotocin-induced diabetic rat subjected to myocardial infarction. Rats were divided randomly into six groups: control sham (CS) + Ad.LacZ, control myocardial infarction (CMI) + Ad.LacZ, control MI + Ad.HSPA12B, diabetic sham (DS) + Ad.LacZ, diabetic MI + Ad.LacZ and diabetic MI + Ad.HSPA12B. Following MI or sham surgery, the respective groups received either Ad...
March 9, 2017: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28270496/jab1-csn5-thioredoxin-signaling-in-relapsed-acute-monocytic-leukemia-under-oxidative-stress
#5
Fuling Zhou, Yunbao Pan, Yongchang Wei, Ronghua Zhang, Gaigai Bai, Qiuju Shen, Shan Meng, Xiao-Feng Le, Michael Andreeff, Francois X Claret
PURPOSE: High levels of ROS and ineffective antioxidant systems contribute to oxidative stress, which affects the function of hematopoietic cells in acute myeloid leukemia (AML); however, the mechanisms by which ROS lead to malignant transformation in relapsed AML-M5 are not completely understood. We hypothesized that alterations in intracellular ROS would trigger AML-M5 relapse by activating the intrinsic pathway. EXPERIMENTAL DESIGN: We studied ROS levels and conducted JAB1/COPS5 and TRX gene expression analyses with blood samples obtained from 60 matched AML-M5 patients at diagnosis and relapse and conducted mechanism studies of Jab1's regulation of Trx in leukemia cell lines...
March 7, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28253042/monitoring-elite-soccer-players-external-loads-using-real-time-data
#6
Steve Barrett
PURPOSE: The principle aim of the study was to assess the validity of measuring locomotor activities and PlayerLoad using Real-Time (RT) data collection during soccer training. METHODS: Twenty-nine (n=29) English soccer players participated. Each player wore the same MEMS device (S5, Optimeye, CatapultSports, Melbourne, Australia) during twenty-one training sessions (n= 331 data sets) in the 2015/2016 and 2016/2017 season. A Real-Time receiver (TRX, Catapultsports, Melbourne, Australia) was used to collect the locomotor activities and PlayerLoad data in RT and compared with the post-event downloaded (PED) data...
March 2, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/28251482/analysis-of-the-interactions-between-thioredoxin-and-20-selenoproteins-in-chicken
#7
Qi Liu, Jie Yang, Jingzeng Cai, Yilin Luan, Hamid Sattar, Man Liu, Shiwen Xu, Ziwei Zhang
Thioredoxin (Trx) is a small molecular protein with complicated functions in a number of processes, including inflammation, apoptosis, embryogenesis, cardiovascular disease, and redox regulation. Some selenoproteins, such as glutathione peroxidase (Gpx), iodothyronine deiodinase (Dio), and thioredoxin reductase (TR), are involved in redox regulation. However, whether there are interactions between Trx and selenoproteins is still not known. In the present paper, we used a Modeller, Hex 8.0.0, and the KFC2 Server to predict the interactions between Trx and selenoproteins...
March 2, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28246333/distinct-electron-transfer-from-ferredoxin-thioredoxin-reductase-to-multiple-thioredoxin-isoforms-in-chloroplasts
#8
Keisuke Yoshida, Toru Hisabori
Thiol-based redox regulation is considered to support light-responsive control of various chloroplast functions. The redox cascade via ferredoxin-thioredoxin reductase (FTR)/thioredoxin (Trx) has been recognized as a key to transmitting reducing power; however, the Arabidopsis thaliana genome sequencing has revealed that as many as five Trx subtypes encoded by a total of ten nuclear genes are targeted to chloroplasts. Because each Trx isoform seems to have a distinct target selectivity, the electron distribution from FTR to multiple Trxs is thought to be the critical branch point for determining the consequence of chloroplast redox regulation...
February 28, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28228180/age-related-oxidative-stress-and-antioxidant-capacity-in-heat-stressed-broilers
#9
A P Del Vesco, A S Khatlab, E S R Goes, K S Utsunomiya, J S Vieira, A R Oliveira Neto, E Gasparino
We aimed to evaluate the effects of acute heat stress (HS) and age on the redox state in broilers aged 21 and 42 days. We evaluated the expression of genes related to antioxidant capacity, the production of hydrogen peroxide (H2O2), and the activity of antioxidant enzymes in the liver, as well as oxidative stress markers in the liver and plasma. The experiment had a completely randomized factorial design with two thermal environments (thermoneutral and HS, 38°C for 24 h) and two ages (21 and 42 days). Twenty-one-day-old animals exposed to HS showed the highest thioredoxin reductase 1 (TrxR1) (P<0...
February 23, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28214375/anti-fatigue-effects-of-troxerutin-on-exercise-endurance-capacity-oxidative-stress-and-mmp-9-levels-in-trained-male-rats
#10
Mohammad Zamanian, Mohammad R Hajizadeh, Ali Esmaeili Nadimi, Ali Shamsizadeh, Mohammad Allahtavakoli
The aim of this study was to investigate effects of troxerutin (TRX) on endurance capacity, oxidative stress and MMP-9 levels in trained male rats. Forty male Wistar rats were divided into five groups. The control (Vehicle) and exercise training (5 days/week) with vehicle treatment (Exercise), exercise training with TRX treatment at 75 (Ex-TRX75), 150 (Ex-TRX150), and 300 mg/kg (Ex-TRX300). The treated groups received TRX by gavage every day while the other groups received water for 30 days. On the 30(th) day, rats were sacrificed immediately after exhaustive swimming test, and some biochemical parameters were measured...
February 18, 2017: Fundamental & Clinical Pharmacology
https://www.readbyqxmd.com/read/28192930/mitochondria-targeted-and-intracellular-biothiol-triggered-hyperpolarized-129-xe-magnetofluorescent-biosensor
#11
Qingbin Zeng, Qianni Guo, Yaping Yuan, Yuqi Yang, Bin Zhang, Lili Ren, Xiaoxiao Zhang, Qing Luo, Maili Liu, Louis-S Bouchard, Xin Zhou
Biothiols such as gluthathione (GSH), cysteine (Cys), homocysteine (Hcy), and thioredoxin (Trx) play vital roles in cellular metabolism. Various diseases are associated with abnormal cellular biothiol levels. Thus, the intracellular detection of biothiol levels could be a useful diagnostic tool. A number of methods have been developed to detect intracellular thiols, but sensitivity and specificity problems have limited their applications. To address these limitations, we have designed a new biosensor based on hyperpolarized xenon magnetic resonance detection, which can be used to detect biothiol levels noninvasively...
February 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28185719/the-induction-of-oxidative-stress-in-cervix-carcinoma-cells-by-levoglucosenone-derived-4-s-salicyl-derivative-and-1-4-s-thio-disaccharides-part-4
#12
Joanna Sarnik, Anna Czubatka-Bienkowska, Anna Macieja, Roman Bielski, Zbigniew J Witczak, Tomasz Poplawski
(1-4)-S-thiodisaccharides were shown to kill various cancer cell lines, including cervix, lung, mammary-gland and colon by unknown mechanisms. Here we identified two actions of levoglucosenone derived (1-4)-S-thiodisaccharides against cervix cancer cells: induction of oxidative stress and DNA damage. In consequence, (1-4)-S-thiodisaccharides lowered the cellular GSH level and changed the expression profile of genes encoding key proteins involved with oxidative stress response. We also observed that (1-4)-S-thiodisaccharides induced DNA damage and interfered with the thioredoxin (Trx) system...
January 22, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28183062/thioredoxin-trxo1-interacts-with-proliferating-cell-nuclear-antigen-pcna-and-its-overexpression-affects-the-growth-of-tobacco-cell-culture
#13
Aingeru Calderón, Ana Ortiz-Espín, Raquel Iglesias-Fernández, Pilar Carbonero, Federico Vicente Pallardó, Francisca Sevilla, Ana Jiménez
Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions...
April 2017: Redox Biology
https://www.readbyqxmd.com/read/28183007/thioredoxin-and-metallothionein-homeostasis-related-proteins-in-lip-carcinogenesis
#14
Patrícia C Caldeira, Laíssa S Silva, Aline C Batista, Maria Cássia F Aguiar
OBJECTIVE: Thioredoxin (Trx) and metallothionein (MT) are involved in the development of some carcinomas; however, the role of these proteins in labial carcinogenesis has not yet been tested. The aims of the study were to evaluate and to correlate the immunoexpression of Trx and MT in actinic cheilitis, lip squamous cell carcinoma, and normal vermillion lip mucosa. DESIGN: Immunohistochemistry was undertaken for Trx and MT in samples of actinic cheilitis, lip squamous cell carcinoma, and normal lip mucosa...
February 1, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28179506/thioredoxin-reverses-age-related-hypertension-by-chronically-improving-vascular-redox-and-restoring-enos-function
#15
Rob H P Hilgers, Venkatesh Kundumani-Sridharan, Jaganathan Subramani, Leon C Chen, Luis G Cuello, Nancy J Rusch, Kumuda C Das
The incidence of high blood pressure with advancing age is notably high, and it is an independent prognostic factor for the onset or progression of a variety of cardiovascular disorders. Although age-related hypertension is an established phenomenon, current treatments are only palliative but not curative. Thus, there is a critical need for a curative therapy against age-related hypertension, which could greatly decrease the incidence of cardiovascular disorders. We show that overexpression of human thioredoxin (TRX), a redox protein, in mice prevents age-related hypertension...
February 8, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28178212/anti-inflammatory-activity-of-sanghuangporus-sanghuang-mycelium
#16
Wang-Ching Lin, Jeng-Shyan Deng, Shyh-Shyun Huang, Sheng-Hua Wu, Chin-Chu Chen, Wan-Rong Lin, Hui-Yi Lin, Guan-Jhong Huang
Acute lung injury (ALI) is characterized by inflammation of the lung tissue and oxidative injury caused by excessive accumulation of reactive oxygen species. Studies have suggested that anti-inflammatory or antioxidant agents could be used for the treatment of ALI with a good outcome. Therefore, our study aimed to test whether the mycelium extract of Sanghuangporus sanghuang (SS-1), believed to exhibit antioxidant and anti-inflammatory properties, could be used against the excessive inflammatory response associated with lipopolysaccharides (LPS)-induced ALI in mice and to investigate its possible mechanism of action...
February 7, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28168934/a-comparative-evaluation-of-3-different-free-form-deformable-image-registration-and-contour-propagation-methods-for-head-and-neck-mri
#17
Sara Broggi, Elisa Scalco, Maria Luisa Belli, Gerlinde Logghe, Dirk Verellen, Stefano Moriconi, Anna Chiara, Anna Palmisano, Renata Mellone, Claudio Fiorino, Giovanna Rizzo
PURPOSE: To validate and compare the deformable image registration and parotid contour propagation process for head and neck magnetic resonance imaging in patients treated with radiotherapy using 3 different approaches-the commercial MIM, the open-source Elastix software, and an optimized version of it. MATERIALS AND METHODS: Twelve patients with head and neck cancer previously treated with radiotherapy were considered. Deformable image registration and parotid contour propagation were evaluated by considering the magnetic resonance images acquired before and after the end of the treatment...
January 1, 2017: Technology in Cancer Research & Treatment
https://www.readbyqxmd.com/read/28168010/rutin-as-a-mediator-of-lipid-metabolism-and-cellular-signaling-pathways-interactions-in-fibroblasts-altered-by-uva-and-uvb-radiation
#18
Agnieszka Gęgotek, Paula Rybałtowska-Kawałko, Elżbieta Skrzydlewska
Background. Rutin is a natural nutraceutical that is a promising compound for the prevention of UV-induced metabolic changes in skin cells. The aim of this study was to examine the effects of rutin on redox and endocannabinoid systems, as well as proinflammatory and proapoptotic processes, in UV-irradiated fibroblasts. Methods. Fibroblasts exposed to UVA and UVB radiation were treated with rutin. The activities and levels of oxidants/antioxidants and endocannabinoid system components, as well as lipid, DNA, and protein oxidation products, and the proinflammatory and pro/antiapoptotic proteins expression were measured...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28146421/the-hdac-inhibitor-ar42-interacts-with-pazopanib-to-kill-trametinib-dabrafenib-resistant-melanoma-cells-in-vitro-and-in-vivo
#19
Laurence Booth, Jane L Roberts, Cindy Sander, John Lee, John M Kirkwood, Andrew Poklepovic, Paul Dent
Studies focused on the killing of activated B-RAF melanoma cells by the histone deacetylase (HDAC) inhibitor AR42. Compared to other tumor cell lines, PDX melanoma isolates were significantly more sensitive to AR42-induced killing. AR42 and the multi-kinase inhibitor pazopanib interacted to activate: an eIF2α-Beclin1 pathway causing autophagosome formation; an eIF2α-DR4/DR5/CD95 pathway; and an eIF2α-dependent reduction in the expression of c-FLIP-s, MCL-1 and BCL-XL. AR42 did not alter basal chaperone activity but increased the ability of pazopanib to inhibit HSP90, HSP70 and GRP78...
January 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28142057/the-effect-of-classical-swine-fever-virus-ns5a-and-ns5a-mutants-on-oxidative-stress-and-inflammatory-response-in-swine-testicular-cells
#20
Wang Dong, Huifang Lv, Yifan Wang, Xiaomeng Li, Cheng Li, Lu Wang, Chengbao Wang, Kangkang Guo, Yanming Zhang
Infection with classical swine fever virus (CSFV) results in highly significant economic losses; this infection is characterized by being highly contagious and accompanied by hyperthermia and systemic bleeding. Oxidative stress (OS) plays a critical role in the pathological process of viral infection. The function of the nonstructural protein 5A (NS5A) in the pathogenesis of CSFV has not been completely understood. Here, OS and the inflammatory response were studied with NS5A and substitution mutants in swine testicular (ST) cells...
January 13, 2017: Research in Veterinary Science
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