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https://www.readbyqxmd.com/read/28808882/-1-h-13-c-and-15-n-chemical-shift-assignments-of-saccharomyces-cerevisiae-type-1-thioredoxin-in-the-oxidized-state-by-solution-nmr-spectroscopy
#1
Anwar Iqbal, Fabio C L Almeida
Thioredoxins (Trx) are ubiquitous proteins that regulate several biochemical processes inside the cell. Trx is an important player, displaying oxidoreductase activity and helping to keep and regulate the oxidative state of the cellular environment. Trx also participates in the regulation of many cellular functions, such as DNA synthesis, protection against oxidative stress, cell cycle and signal transduction. The oxidized Trx is the target for another set of proteins, such as thioredoxin reductase (TrR), which used the reductive potential of NADPH...
August 14, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/28782589/the-role-of-thioredoxin-1-in-resisting-methamphetamine-induced-rewarding-effect
#2
Mengbing Huang, Lingpin Kong, Lihua Yang, Xiang Li, Xiaoshuang Zhou, Ye Li, Jie Bai
Methamphetamine (METH) is a highly addictive drug of abuse which induces behavioral sensitization and rewarding effects. Thioredoxin-1 (Trx-1) is a redox protein and plays role in regulating activity of transcription factor, such as cAMP responsive element-binding protein (CREB), AP-1, p53, is emerging as an important modulator of neuronal function. It has been reported that Trx-1 is involved in morphine dependence. In this study, we examined the rewarding effect after METH administration by conditioned place preference (CPP) of mice, and detected the levels of dopamine and the activity of cAMP responsive element-binding protein (CREB), the expressions of ΔFosB and cyclin-dependent kinase 5 (CDK5) in the ventral tegmental area (VTA) and nucleus accumbens (NAc) in mice...
August 4, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28780733/brain-derived-neurotrophic-factor-increases-synaptic-protein-levels-via-the-mapk-erk-signaling-pathway-and-nrf2-trx-axis-following-the-transplantation-of-neural-stem-cells-in-a-rat-model-of-traumatic-brain-injury
#3
Tao Chen, Yu Wu, Yuzi Wang, Jigao Zhu, Haiying Chu, Li Kong, Liangwei Yin, Haiying Ma
Brain-derived neurotrophic factor (BDNF) plays an important role in promoting the growth, differentiation, survival and synaptic stability of neurons. Presently, the transplantation of neural stem cells (NSCs) is known to induce neural repair to some extent after injury or disease. In this study, to investigate whether NSCs genetically modified to encode the BDNF gene (BDNF/NSCs) would further enhance synaptogenesis, BDNF/NSCs or naive NSCs were directly engrafted into lesions in a rat model of traumatic brain injury (TBI)...
August 5, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28774816/redox-regulation-of-the-tumor-suppressor-pten-by-the-thioredoxin-system-and-cumene-hydroperoxide
#4
Seong-Jeong Han, Ying Zhang, Inyoung Kim, Kee-Oh Chay, Hyun Joong Yoon, Dong Il Jang, Sung Yeul Yang, Jiyoung Park, Hyun Ae Woo, Iha Park, Seung-Rock Lee
Intracellular redox status influences the oxidation and enzyme activity of the tumor suppressor phosphatase and tensin homolog on chromosome 10 (PTEN). Cumene hydroperoxide (CuHP), an organic hydroperoxide, is a known tumor promoter. However, molecular targets and action mechanism of CuHP in tumor promotion have not been well characterized. In this study, we investigated the effect of CuHP on the redox state of PTEN in HeLa cells. In addition, the intracellular reducing system of oxidized PTEN was analyzed using a biochemical approach and the effect of CuHP on this reducing system was also analyzed...
August 1, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28771858/intrauterine-delivery-of-subunit-vaccines-induces-a-systemic-and-mucosal-immune-response-in-rabbits
#5
Jonathan Alexander Pasternak, Glenn Hamonic, Nikki M Forsberg, Colette L Wheler, Michael K Dyck, Heather L Wilson
PROBLEM: Mucosal vaccines have long been sought after to improve protection though the production of both a mucosal and systemic immune response, and are thought to be particularly effective at the site of induction. Development of such vaccines has, however, been delayed by the general propensity to develop immune tolerance to antigens encountered at mucosal sites. This study aimed to determine whether an appropriately formulated subunit vaccine delivered to the uterine lumen would effectively trigger induction of immunity over tolerance...
August 3, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28757467/high-level-expression-and-purification-of-soluble-form-of-human-natural-killer-cell-receptor-nkr-p1-in-hek293s-gnti-cells
#6
Jan Bláha, Barbora Kalousková, Ondřej Skořepa, Samuel Pažický, Petr Novák, Ondřej Vaněk
Human natural killer receptor protein 1 (NKR-P1, CD161, gene klrb1) is a C-type lectin-like receptor of natural killer (NK) cells responsible for recognition of its cognate protein ligand lectin-like transcript 1 (LLT1). NKR-P1 is the single human orthologue of the prototypical rodent NKR-P1 receptors. Naturally, human NKR-P1 is expressed on the surface of NK cells, where it serves as inhibitory receptor; and on T and NKT cells functioning as co-stimulatory receptor promoting secretion of IFNγ. Most notably, it is expressed on Th17 and Tc17 lymphocytes where presumably promotes targeting into LLT1 expressing immunologically privileged niches...
July 27, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28757135/synergism-between-thioredoxin-reductase-inhibitor-ethaselen-and-sodium-selenite-in-inhibiting-proliferation-and-inducing-death-of-human-non-small-cell-lung-cancer-cells
#7
Xiaoqing Zheng, Wei Xu, Ruoxuan Sun, Hanwei Yin, Chaoran Dong, Huihui Zeng
New effective treatment for human non-small cell lung cancer (NSCLC) is needed. The thioredoxin (Trx) system composes of thioredoxin reductase (TrxR), Trx and NADPH. In this study, we combined an organic selenium compound--TrxR inhibitor ethaselen (BBSKE) with low dosage sodium selenite to inhibit proliferation and induce death of NSCLC cells, and identified underlying mechanisms. Synergistic anti-proliferation effect of BBSKE and selenite was found in human NSCLC cell lines, A549, NCI-H1299 and NCI-1266. A significant increase of apoptosis, necrosis and autophagy were observed in the group of BBSKE plus selenite in A549 cells...
July 27, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28755477/thioredoxin-interacting-protein-induced-%C3%AE-synuclein-accumulation-via-inhibition-of-autophagic-flux-implications-for-parkinson-s-disease
#8
Cun-Jin Su, Yu Feng, Teng-Teng Liu, Xu Liu, Jun-Jie Bao, Ai-Ming Shi, Duan-Min Hu, Tong Liu, Yun-Li Yu
AIMS: Thioredoxin-interacting protein (TXNIP) is associated with activation of oxidative stress through inhibition of thioredoxin (Trx). However, some evidences point out that TXNIP acts as a scaffolding protein in signaling complex independent of cellular redox regulation. The autophagy-lysosomal pathway plays important roles in the clearance of misfolded proteins and dysfunctional organelles. Lysosomal dysfunction has been involved in several neurodegenerative disorders including Parkinson's disease (PD)...
July 29, 2017: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/28751915/gene-expression-and-molecular-characterization-of-a-xylanase-from-chicken-cecum-metagenome
#9
Hind Al-Darkazali, Vithaya Meevootisom, Duangnate Isarangkul, Suthep Wiyakrutta
A xylanase gene xynAMG1 with a 1,116-bp open reading frame, encoding an endo-β-1,4-xylanase, was cloned from a chicken cecum metagenome. The translated XynAMG1 protein consisted of 372 amino acids including a putative signal peptide of 23 amino acids. The calculated molecular mass of the mature XynAMG1 was 40,013 Da, with a theoretical pI value of 5.76. The amino acid sequence of XynAMG1 showed 59% identity to endo-β-1,4-xylanase from Prevotella bryantii and Prevotella ruminicola and 58% identity to that from Prevotella copri...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28735961/binding-of-pmclipsp2-to-microbial-cell-wall-components-and-activation-of-the-propo-activating-system-in-the-black-tiger-shrimp-penaeus-monodon
#10
Narach Khorattanakulchai, Piti Amparyup, Anchalee Tassanakajon
Clip domain serine proteinases (ClipSPs) play an important role in the prophenoloxidase-activating (proPO) system. In the shrimp Penaeus monodon, the ClipSP PmClipSP2 has been previously shown to bind to microbial polysaccharides (LPS and β-1,3-glucan) and likely activates the proPO system. To reveal the binding site of the PmClipSP2 protein, the N-terminal clip domain (Clip-PmClipSP) and C-terminal SP domain (SP-PmClipSP2) were separately cloned. The recombinant proteins were then assayed for their binding properties and involvement in proPO activation...
July 20, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28734839/recombinant-expression-of-ixolaris-a-kunitz-type-inhibitor-from-the-tick-salivary-gland-for-nmr-studies
#11
V S De Paula, F H S Silva, I M B Francischetti, R Q Monteiro, A P Valente
Ixolaris is an anticoagulant protein identified in the tick saliva of Ixodes scapularis. Ixolaris contains 2 Kunitz like domains and binds to Factor Xa or Factor X as a scaffold for inhibition of the Tissue Factor (TF)/Factor VIIa (FVIIa). In contrast to tissue factor pathway inhibitor (TFPI), however, Ixolaris does not bind to the active site cleft of FXa. Instead, complex formation is mediated by the FXa heparin-binding exosite. Due to its potent and long-lasting antithrombotic activity, Ixolaris is a promising agent for anticoagulant therapy...
July 19, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28712392/-protection-of-rho-associated-protein-kinase-inhibitor-y-27632-in-mrl-lpr-mice
#12
Yuanyuan Wang, Xiaoyun Jia, Qiyi Wang, Ming Ye, Yang Lu, Jiajia Zhang, Sheng Chen, Yanglei Li, Junying Wu, Changhao Xie
Objective To investigate the immunological effect of Rho-associated protein kinase inhibitor Y-27632 on MRL/lpr mice and explore its underlying mechanisms. Methods The study collected 10 wild-type C57BL/6 mice as normal controls and 20 MRL/lpr mice that were randomly assigned to MRL/lpr group and 5 mg/kg Y-27632-treated MRL/lpr group, with 10 mice in each group. Superoxide dismutase (SOD), methane dicarboxylic aldehyde (MDA), interleukin 6 (IL-6), IL-1β and tumor necrosis factor α (TNF-α) in the serum or spleen were detected by ELISA...
July 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28706574/neuronal-damage-induced-by-perinatal-asphyxia-is-attenuated-by-postinjury-glutaredoxin-2-administration
#13
Juan Ignacio Romero, Mariana Inés Holubiec, Tamara Logica Tornatore, Stéphanie Rivière, Eva-Maria Hanschmann, Rodolfo Alberto Kölliker-Frers, Julia Tau, Eduardo Blanco, Pablo Galeano, Fernando Rodríguez de Fonseca, Christopher Horst Lillig, Francisco Capani
The general disruption of redox signaling following an ischemia-reperfusion episode has been proposed as a crucial component in neuronal death and consequently brain damage. Thioredoxin (Trx) family proteins control redox reactions and ensure protein regulation via specific, oxidative posttranslational modifications as part of cellular signaling processes. Trx proteins function in the manifestation, progression, and recovery following hypoxic/ischemic damage. Here, we analyzed the neuroprotective effects of postinjury, exogenous administration of Grx2 and Trx1 in a neonatal hypoxia/ischemia model...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28698569/ahpc-of-the-mycobacterial-antioxidant-defense-system-and-its-interaction-with-its-reducing-partner-thioredoxin-c
#14
Chui Fann Wong, Joon Shin, Malathy Sony Subramanian Manimekalai, Wuan Geok Saw, Zhan Yin, Shashi Bhushan, Arvind Kumar, Priya Ragunathan, Gerhard Grüber
Despite the highly oxidative environment of the phagosomal lumen, the need for maintaining redox homeostasis is a critical aspect of mycobacterial biology. The pathogens are equipped with the sophisticated thioredoxin- (Trx) and peroxiredoxin system, including TrxC and the alkyl hydroperoxide reductase subunit C (AhpC), whereby TrxC is one of the reducing partners of AhpC. Here we visualize the redox modulated dodecamer ring formation of AhpC from Mycobacterium bovis (BCG strain; MbAhpC) using electron microscopy and present novel insights into the unique N-terminal epitope (40 residues) of mycobacterial AhpC...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694441/the-glyceraldehyde-3-phosphate-dehydrogenase-gapdh-of-corynebacterium-diphtheriae-is-redox-controlled-by-protein-s-mycothiolation-under-oxidative-stress
#15
Melanie Hillion, Marcel Imber, Brandán Pedre, Jörg Bernhardt, Malek Saleh, Vu Van Loi, Sandra Maaß, Dörte Becher, Leonardo Astolfi Rosado, Lorenz Adrian, Christoph Weise, Rüdiger Hell, Markus Wirtz, Joris Messens, Haike Antelmann
Mycothiol (MSH) is the major low molecular weight (LMW) thiol in Actinomycetes and functions in post-translational thiol-modification by protein S-mycothiolation as emerging thiol-protection and redox-regulatory mechanism. Here, we have used shotgun-proteomics to identify 26 S-mycothiolated proteins in the pathogen Corynebacterium diphtheriae DSM43989 under hypochlorite stress that are involved in energy metabolism, amino acid and nucleotide biosynthesis, antioxidant functions and translation. The glyceraldehyde-3-phosphate dehydrogenase (GapDH) represents the most abundant S-mycothiolated protein that was modified at its active site Cys153 in vivo...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28692378/photosynthetic-activity-of-cotyledons-is-critical-during-post-germinative-growth-and-seedling-establishment
#16
Valle Ojeda, Victoria A Nájera, Maricruz González, Juan M Pérez-Ruiz, Francisco J Cejudo
Thioredoxins (Trxs) play a relevant role in thiol-dependent redox regulation, which allows the rapid adaptation of chloroplast metabolism to unpredictable environmental conditions. In chloroplasts, Trxs use reducing equivalents provided by photoreduced ferredoxin (Fdx) via the action of a ferredoxin-thioredoxin reductase (FTR), thus linking redox regulation to light. In addition, these organelles contain an NADPH-thioredoxin reductase, NTRC, with a Trx domain at the C-terminus. NTRC efficiently reduces 2-Cys peroxiredoxins (Prxs), hence having antioxidant function...
July 10, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28688762/thioredoxin-attenuates-oxidized-low-density-lipoprotein-induced-oxidative-stress-in-human-umbilical-vein-endothelial-cells-by-reducing-nadph-oxidase-activity
#17
Beidong Chen, Li Meng, Tao Shen, Huan Gong, Ruomei Qi, Yanyang Zhao, Jie Sun, Li Bao, Gexin Zhao
Oxidative stress is recognized as one of the most important contributing factors to the development of atherosclerosis. Oxidized low-density lipoprotein (ox-LDL) can induce vascular reactive oxygen species (ROS) production, trigger endothelial dysfunction and initiate the progression of atherosclerosis. Previous studies have demonstrated that thioredoxin-1 (Trx) is one of the key regulators of intracellular redox, which is pivotal in atherogenesis. However, the regulation mechanism is still unclear. In this study, we investigated the effects of Trx1 on NADPH oxidase in human umbilical vein endothelial cells (HUVECs), whose ROS level is mainly produced by NADPH oxidase, especially Nox4 isoform...
September 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28680816/redox-modulation-of-thimet-oligopeptidase-activity-by-hydrogen-peroxide
#18
Marcelo Y Icimoto, Juliana C Ferreira, César H Yokomizo, Larissa V Bim, Alyne Marem, Joyce M Gilio, Vitor Oliveira, Iseli L Nantes
Thimet oligopeptidase (EC 3.4.24.15, TOP) is a cytosolic mammalian zinc protease that can process a diversity of bioactive peptides. TOP has been pointed out as one of the main postproteasomal enzymes that process peptide antigens in the MHC class I presentation route. In the present study, we describe a fine regulation of TOP activity by hydrogen peroxide (H2O2). Cells from a human embryonic kidney cell line (HEK293) underwent an ischemia/reoxygenation-like condition known to increase H2O2 production. Immediately after reoxygenation, HEK293 cells exhibited a 32% increase in TOP activity, but no TOP activity was observed 2 h after reoxygenation...
July 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28680529/mir-382-contributes-to-renal-tubulointerstitial-fibrosis-by-downregulating-hspd1
#19
Yi Fang, Ting Xie, Ning Xue, Qing Kuang, Zheng Wei, Mingyu Liang, Xiaoqiang Ding
Redox imbalance plays an important role in the pathogenesis of CKD progression. Previously, we demonstrated that microRNA-382 (miR-382) contributed to TGF-β1-induced loss of epithelial polarity in human kidney epithelial cells, but its role in the development of renal tubulointerstitial fibrosis remains unknown. In this study, we found that with 7 days of unilateral ureteral obstruction (UUO) in mice, the abundance of miR-382 in the obstructed kidney was significantly increased. Meanwhile, the protein expression of heat shock protein 60 (HSPD1), a predicted target of miR-382, was reduced after 7 days of UUO...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28671680/monitoring-thioredoxin-redox-with-a-genetically-encoded-red-fluorescent-biosensor
#20
Yichong Fan, Merna Makar, Michael X Wang, Hui-Wang Ai
Thioredoxin (Trx) is one of the two major thiol antioxidants, playing essential roles in redox homeostasis and signaling. Despite its importance, there is a lack of methods for monitoring Trx redox dynamics in live cells, hindering a better understanding of physiological and pathological roles of the Trx redox system. In this work, we developed the first genetically encoded fluorescent biosensor for Trx redox by engineering a redox relay between the active-site cysteines of human Trx1 and rxRFP1, a redox-sensitive red fluorescent protein...
June 26, 2017: Nature Chemical Biology
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