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https://www.readbyqxmd.com/read/28934716/automatic-detection-of-micronuclei-by-cell-microscopic-image-processing
#1
Mohammad Taghi Bahreyni Toossi, Hosein Azimian, Omid Sarrafzadeh, Shokoufeh Mohebbi, Shokouhozaman Soleymanifard
With the development and applications of ionizing radiation in medicine, the radiation effects on human health get more and more attention. Ionizing radiation can lead to various forms of cytogenetic damage, including increased frequencies of micronuclei (MNi) and chromosome abnormalities. The cytokinesis block micronucleus (CBMN) assay is widely used method for measuring MNi to determine chromosome mutations or genome instability in cultured human lymphocytes. The visual scoring of MNi is time-consuming and scorer fatigue can lead to inconsistency...
August 12, 2017: Mutation Research
https://www.readbyqxmd.com/read/28934481/automatic-identification-of-informative-regions-with-epigenomic-changes-associated-to-hematopoiesis
#2
Enrique Carrillo-de-Santa-Pau, David Juan, Vera Pancaldi, Felipe Were, Ignacio Martin-Subero, Daniel Rico, Alfonso Valencia
Hematopoiesis is one of the best characterized biological systems but the connection between chromatin changes and lineage differentiation is not yet well understood. We have developed a bioinformatic workflow to generate a chromatin space that allows to classify 42 human healthy blood epigenomes from the BLUEPRINT, NIH ROADMAP and ENCODE consortia by their cell type. This approach let us to distinguish different cells types based on their epigenomic profiles, thus recapitulating important aspects of human hematopoiesis...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28934310/sanger-and-next-generation-sequencing-data-for-characterization-of-ctl-epitopes-in-archived-hiv-1-proviral-dna
#3
Camille Tumiotto, Lionel Riviere, Pantxika Bellecave, Patricia Recordon-Pinson, Alice Vilain-Parce, Gwenda-Line Guidicelli, Hervé Fleury
One of the strategies for curing viral HIV-1 is a therapeutic vaccine involving the stimulation of cytotoxic CD8-positive T cells (CTL) that are Human Leucocyte Antigen (HLA)-restricted. The lack of efficiency of previous vaccination strategies may have been due to the immunogenic peptides used, which could be different from a patient's virus epitopes and lead to a poor CTL response. To counteract this lack of specificity, conserved epitopes must be targeted. One alternative is to gather as many data as possible from a large number of patients on their HIV-1 proviral archived epitope variants, taking into account their genetic background to select the best presented CTL epitopes...
2017: PloS One
https://www.readbyqxmd.com/read/28934302/predicting-couple-therapy-outcomes-based-on-speech-acoustic-features
#4
Md Nasir, Brian Robert Baucom, Panayiotis Georgiou, Shrikanth Narayanan
Automated assessment and prediction of marital outcome in couples therapy is a challenging task but promises to be a potentially useful tool for clinical psychologists. Computational approaches for inferring therapy outcomes using observable behavioral information obtained from conversations between spouses offer objective means for understanding relationship dynamics. In this work, we explore whether the acoustics of the spoken interactions of clinically distressed spouses provide information towards assessment of therapy outcomes...
2017: PloS One
https://www.readbyqxmd.com/read/28934238/a-tool-to-automatically-analyze-electromagnetic-tracking-data-from-high-dose-rate-brachytherapy-of-breast-cancer-patients
#5
Th I Götz, G Lahmer, V Strnad, Ch Bert, B Hensel, A M Tomé, E W Lang
During High Dose Rate Brachytherapy (HDR-BT) the spatial position of the radiation source inside catheters implanted into a female breast is determined via electromagnetic tracking (EMT). Dwell positions and dwell times of the radiation source are established, relative to the patient's anatomy, from an initial X-ray-CT-image. During the irradiation treatment, catheter displacements can occur due to patient movements. The current study develops an automatic analysis tool of EMT data sets recorded with a solenoid sensor to assure concordance of the source movement with the treatment plan...
2017: PloS One
https://www.readbyqxmd.com/read/28934170/mobile-based-analysis-of-malaria-infected-thin-blood-smears-automated-species-and-life-cycle-stage-determination
#6
Luís Rosado, José M Correia da Costa, Dirk Elias, Jaime S Cardoso
Microscopy examination has been the pillar of malaria diagnosis, being the recommended procedure when its quality can be maintained. However, the need for trained personnel and adequate equipment limits its availability and accessibility in malaria-endemic areas. Rapid, accurate, accessible diagnostic tools are increasingly required, as malaria control programs extend parasite-based diagnosis and the prevalence decreases. This paper presents an image processing and analysis methodology using supervised classification to assess the presence of malaria parasites and determine the species and life cycle stage in Giemsa-stained thin blood smears...
September 21, 2017: Sensors
https://www.readbyqxmd.com/read/28933906/neural-bases-of-automaticity
#7
Mathieu Servant, Peter Cassey, Geoffrey F Woodman, Gordon D Logan
Automaticity allows us to perform tasks in a fast, efficient, and effortless manner after sufficient practice. Theories of automaticity propose that across practice processing transitions from being controlled by working memory to being controlled by long-term memory retrieval. Recent event-related potential (ERP) studies have sought to test this prediction, however, these experiments did not use the canonical paradigms used to study automaticity. Specifically, automaticity is typically studied using practice regimes with consistent mapping between targets and distractors and spaced practice with individual targets, features that these previous studies lacked...
September 21, 2017: Journal of Experimental Psychology. Learning, Memory, and Cognition
https://www.readbyqxmd.com/read/28933724/an-automatic-multi-target-independent-analysis-framework-for-non-planar-infrared-visible-registration
#8
Xinglong Sun, Tingfa Xu, Jizhou Zhang, Zishu Zhao, Yuankun Li
In this paper, we propose a novel automatic multi-target registration framework for non-planar infrared-visible videos. Previous approaches usually analyzed multiple targets together and then estimated a global homography for the whole scene, however, these cannot achieve precise multi-target registration when the scenes are non-planar. Our framework is devoted to solving the problem using feature matching and multi-target tracking. The key idea is to analyze and register each target independently. We present a fast and robust feature matching strategy, where only the features on the corresponding foreground pairs are matched...
July 26, 2017: Sensors
https://www.readbyqxmd.com/read/28933707/high-accuracy-automatic-classification-of-parkinsonian-tremor-severity-using-machine-learning-method
#9
Hyoseon Jeon, Woongwoo Lee, Hyeyoung Park, Hong Ji Lee, Sang Kyong Kim, Han Byul Kim, Beomseok Jeon, Kwang Suk Park
Although clinical aspirations for new technology to accurately measure and diagnose Parkinsonian tremors exist, automatic scoring of tremor severity using machine learning approaches has not yet been employed. Objective: This study aims to maximize the scientific validity of automatic tremor-severity classification using machine learning algorithms to score Parkinsonian tremor severity in the same manner as the Unified Parkinson's Disease Rating Scale (UPDRS) used to rate scores in real clinical practice...
September 21, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28933556/the-assembly-behavior-of-organically-interlinked-gold-nanoparticle-composite-films-a-quartz-crystal-microbalance-investigation
#10
Yelyena Daskal, Tina Tauchnitz, Frederic Güth, Rosemarie Dittrich, Yvonne Joseph
Thin films based on dodecylamine stabilized gold nanoparticles interlinked with different organic molecules are prepared by automatic layer-by-layer self-assembly in a microfluidic quartz crystal microbalance (QCM) cell, to obtain an in-situ insight on the film formation by ligand/linker exchange reactions. The influence of interlinking functional groups and the length of the organic linker molecule on assembly behavior is investigated. Namely alkyldithiols with different lengths are compared to alkyldiamines and alkylbisdithiocarbamates with a C8 alkylic molecular backbone...
September 21, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28932696/an-automated-workflow-for-quantifying-rna-transcripts-in-individual-cells-in-large-data-sets
#11
Matthew C Pharris, Tzu-Ching Wu, Xinping Chen, Xu Wang, David M Umulis, Vikki M Weake, Tamara L Kinzer-Ursem
Advanced molecular probing techniques such as single molecule fluorescence in situ hybridization (smFISH) or RNAscope can be used to assess the quantity and spatial location of mRNA transcripts within cells. Quantifying mRNA expression in large image sets usually involves automated counting of fluorescent spots. Though conventional spot counting algorithms may suffice, they often lack high-throughput capacity and accuracy in cases of crowded signal or excessive noise. Automatic identification of cells and processing of many images is still a challenge...
2017: MethodsX
https://www.readbyqxmd.com/read/28932653/a-bio-inspired-approach-for-the-design-of-a-multifunctional-robotic-end-effector-customized-for-automated-maintenance-of-a-reconfigurable-vibrating-screen
#12
O A Makinde, K Mpofu, R Vrabic, B I Ramatsetse
The development of a robotic-driven maintenance solution capable of automatically maintaining reconfigurable vibrating screen (RVS) machine when utilized in dangerous and hazardous underground mining environment has called for the design of a multifunctional robotic end-effector capable of carrying out all the maintenance tasks on the RVS machine. In view of this, the paper presents a bio-inspired approach which unfolds the design of a novel multifunctional robotic end-effector embedded with mechanical and control mechanisms capable of automatically maintaining the RVS machine...
2017: Robotics and Biomimetics
https://www.readbyqxmd.com/read/28932179/non-invasive-real-time-monitoring-of-yeast-volatilome-by-ptr-tof-ms
#13
Iuliia Khomenko, Irene Stefanini, Luca Cappellin, Valentina Cappelletti, Pietro Franceschi, Duccio Cavalieri, Tilmann D Märk, Franco Biasioli
INTRODUCTION: Producing a wide range of volatile secondary metabolites Saccharomyces cerevisiae influences wine, beer, and bread sensory quality and hence selection of strains based on their volatilome becomes pivotal. A rapid on-line method for volatilome assessing of strains growing on standard solid media is still missing. OBJECTIVES: Methodologically, the aim of this study was to demonstrate the automatic, real-time, direct, and non-invasive monitoring of yeast volatilome in order to rapidly produce a robust large data set encompassing measurements relative to many strains, replicates and time points...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28932019/towards-identifying-dyslexia-in-standard-indonesian-the-development-of-a-reading-assessment-battery
#14
Bernard A J Jap, Elisabeth Borleffs, Ben A M Maassen
With its transparent orthography, Standard Indonesian is spoken by over 160 million inhabitants and is the primary language of instruction in education and the government in Indonesia. An assessment battery of reading and reading-related skills was developed as a starting point for the diagnosis of dyslexia in beginner learners. Founded on the International Dyslexia Association's definition of dyslexia, the test battery comprises nine empirically motivated reading and reading-related tasks assessing word reading, pseudoword reading, arithmetic, rapid automatized naming, phoneme deletion, forward and backward digit span, verbal fluency, orthographic choice (spelling), and writing...
2017: Reading and Writing
https://www.readbyqxmd.com/read/28931888/automatic-classification-of-cancerous-tissue-in-laserendomicroscopy-images-of-the-oral-cavity-using-deep-learning
#15
Marc Aubreville, Christian Knipfer, Nicolai Oetter, Christian Jaremenko, Erik Rodner, Joachim Denzler, Christopher Bohr, Helmut Neumann, Florian Stelzle, Andreas Maier
Oral Squamous Cell Carcinoma (OSCC) is a common type of cancer of the oral epithelium. Despite their high impact on mortality, sufficient screening methods for early diagnosis of OSCC often lack accuracy and thus OSCCs are mostly diagnosed at a late stage. Early detection and accurate outline estimation of OSCCs would lead to a better curative outcome and a reduction in recurrence rates after surgical treatment. Confocal Laser Endomicroscopy (CLE) records sub-surface micro-anatomical images for in vivo cell structure analysis...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931809/automatic-and-adaptive-heterogeneous-refractive-index-compensation-for-light-sheet-microscopy
#16
Duncan P Ryan, Elizabeth A Gould, Gregory J Seedorf, Omid Masihzadeh, Steven H Abman, Sukumar Vijayaraghavan, Wendy B Macklin, Diego Restrepo, Douglas P Shepherd
Optical tissue clearing has revolutionized researchers' ability to perform fluorescent measurements of molecules, cells, and structures within intact tissue. One common complication to all optically cleared tissue is a spatially heterogeneous refractive index, leading to light scattering and first-order defocus. We designed C-DSLM (cleared tissue digital scanned light-sheet microscopy) as a low-cost method intended to automatically generate in-focus images of cleared tissue. We demonstrate the flexibility and power of C-DSLM by quantifying fluorescent features in tissue from multiple animal models using refractive index matched and mismatched microscope objectives...
September 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28931626/pacequant-a-tool-for-high-throughput-quantification-of-pavement-cell-shape-characteristics
#17
Birgit Möller, Yvonne Poeschl, Romina Plötner, Katharina Bürstenbinder
Pavement cells (PCs) are the most frequently occurring cell type in the leaf epidermis and play important roles in leaf growth and function. In many plant species, PCs form highly complex jigsaw puzzle shaped cells with interlocking lobes. Understanding of their development is of high interest for plant science research because of their importance for leaf growth and hence for plant fitness and crop yield. Studies of PC development, however, are limited because robust methods are lacking that enable automatic segmentation and quantification of PC shape parameters suitable to reflect their cellular complexity...
September 20, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28931455/the-effect-of-concentrate-supplementation-on-milk-production-and-cow-traffic-in-early-and-late-lactation-in-a-pasture-based-automatic-milking-system
#18
J Shortall, C Foley, R D Sleator, B O'Brien
The objective of this experiment was to establish the effect of low-concentrate (LC) and high-concentrate (HC) supplementation in the early and late periods of lactation on milk production and cow traffic in a pasture-based automatic milking (AM) system. In total, 40 cows (10 primiparous and 30 multiparous) were randomly assigned to one of the two treatments. The experimental periods for the early and late lactation trials extended from 23 February to 12 April 2015 and 31 August to 18 October 2015, respectively (49 days in each trial period)...
September 21, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28931305/dental-flossing-and-automaticity-a-longitudinal-moderated-mediation-analysis
#19
Kyra Hamilton, Sheina Orbell, Mikaela Bonham, Jeroen Kroon, Ralf Schwarzer
We investigated the role of normative support, behavioural automaticity, and action control in predicting dental flossing behaviour. Between May and October 2015, 629 Australian young adults completed a questionnaire assessing constructs of normative support and automaticity, and a 2-week follow-up of dental flossing behaviour and action control, resulting in n = 241 persons for longitudinal analysis. Findings supported the hypotheses that the effect of normative support on behaviour would be mediated via automaticity, and the effect of automaticity would be moderated by action control...
September 20, 2017: Psychology, Health & Medicine
https://www.readbyqxmd.com/read/28931067/in-vivo-imaging-system-for-explants-analysis-a-new-approach-for-assessment-of-cell-transplantation-effects-in-large-animal-models
#20
Weronika Zarychta-Wiśniewska, Anna Burdzinska, Radosław Zagozdzon, Bartosz Dybowski, Marta Butrym, Zdzisław Gajewski, Leszek Paczek
INTRODUCTION: Despite spectacular progress in cellular transplantology, there are still many concerns about the fate of transplanted cells. More preclinical studies are needed, especially on large animal models, to bridge the translational gap between basic research and the clinic. Herein, we propose a novel approach in analysis of cell transplantation effects in large animals explants using in vivo imaging system (IVIS®) or similar equipment. MATERIAL AND METHODS: In the in vitro experiment cells labeled with fluorescent membrane dyes: DID (far red) or PKH26 (orange) were visualized with IVIS®...
2017: PloS One
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