Liang Xue, Pengcheng Wang, Lianshui Wang, Emily Renzi, Predrag Radivojac, Haixu Tang, Randy Arnold, Jian-Kang Zhu, W Andy Tao
Global phosphorylation changes in plants in response to environmental stress have been relatively poorly characterized to date. Here we introduce a novel mass spectrometry-based label-free quantitation method that facilitates systematic profiling plant phosphoproteome changes with high efficiency and accuracy. This method employs synthetic peptide libraries tailored specifically as internal standards for complex phosphopeptide samples and accordingly, a local normalization algorithm, LAXIC, which calculates phosphopeptide abundance normalized locally with co-eluting library peptides...
August 2013: Molecular & Cellular Proteomics: MCP