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mitosis in melanoma

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https://www.readbyqxmd.com/read/27873142/university-hospital-waterford-5-year-experience-of-cutaneous-melanoma
#1
R Sehgal, C X Cheung, R Alradadi, D A Healy, R Landers, G T O'Donoghue
INTRODUCTION: The incidence of cutaneous melanoma (CM) continues to rise in Ireland. Despite significant advances in melanoma molecular therapy, surgery remains the mainstay of treatment for CM. The University Hospital Waterford (UHW) prospectively maintained CM registry was established in 2010. AIM: To summarize 5-year experience (2010-2015) of primary CM presenting to UHW. METHODS: Data were retrospectively obtained from a central electronic pathology and radiology repository augmented by HIPE data and theatre logs...
November 21, 2016: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/27835901/mutational-activation-of-braf-confers-sensitivity-to-transforming-growth-factor-beta-inhibitors-in-human-cancer-cells
#2
Lindsay C Spender, G John Ferguson, Sijia Liu, Chao Cui, Maria Romina Girotti, Gary Sibbet, Ellen B Higgs, Morven K Shuttleworth, Tom Hamilton, Paul Lorigan, Michael Weller, David F Vincent, Owen J Sansom, Margaret Frame, Peter Ten Dijke, Richard Marais, Gareth J Inman
Recent data implicate elevated transforming growth factor-β (TGFβ) signalling in BRAF inhibitor drug-resistance mechanisms, but the potential for targeting TGFβ signalling in cases of advanced melanoma has not been investigated. We show that mutant BRAFV600E confers an intrinsic dependence on TGFβ/TGFβ receptor 1 (TGFBR1) signalling for clonogenicity of murine melanocytes. Pharmacological inhibition of the TGFBR1 blocked the clonogenicity of human mutant BRAF melanoma cells through SMAD4-independent inhibition of mitosis, and also inhibited metastasis in xenografted zebrafish...
November 9, 2016: Oncotarget
https://www.readbyqxmd.com/read/27613330/invitro-and-in-vivo-antitumor-activity-of-a-novel-carbonyl-ruthenium-compound-the-ct-rucl-co-dppb-bipy-pf-6-dppb-1-4-bis-diphenylphosphine-butane-and-bipy-2-2-bipyridine
#3
Andréa P Carnizello, Marília I F Barbosa, Monize Martins, Natália H Ferreira, Pollyanna F Oliveira, Geórgia M Magalhães, Alzir A Batista, Denise C Tavares
This study performed in vitro and in vivo biological assays of the ruthenium (II) compound ct-[RuCl(CO)(dppb)(bipy)]PF6 (where, dppb=1,4-bis(diphenylphosphine)butane and bipy=2,2'-bipyridine). The cytotoxic activity of this compound was evaluated against different tumor cell lines (HeLa, human cervical adenocarcinoma; MCF7, human breast adenocarcinoma; MO59J, human glioblastoma; HepG2, hepatocellular carcinoma and B16F10, murine melanoma) and healthy cell line (V79, Chinese hamster lung fibroblasts), by XTT (sodium 2,3'-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl]-3,4-tetrazolium-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) method...
August 25, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27602146/an-observational-study-regarding-the-rate-of-growth-in-vertical-and-radial-growth-phase-superficial-spreading-melanomas
#4
Roberto Betti, Elena Agape, Raffaella Vergani, Laura Moneghini, Amilcare Cerri
The natural history of superficial spreading melanomas (SSMs) involves the progression from a radial growth phase (RGP) to a vertical growth phase (VGP). Currently, a patient's history represents the only method to estimate the rate of tumor growth. The present study aimed to verify whether the estimated rate of growth (ROG) of SSMs with a RGP or VGP exhibited any differences, and to evaluate the possible implications for the most important prognostic determinants. ROG was quantified as the ratio between Breslow's thickness in millimeters (mm) and the time of tumor growth in months, defined as the time between the date that the patient had first noticed the lesion in which melanoma subsequently developed and the date on which the patient first felt this lesion changed...
September 2016: Oncology Letters
https://www.readbyqxmd.com/read/27554118/p21-is-responsible-for-ionizing-radiation-induced-bypass-of-mitosis
#5
Xu Rui Zhang, Yong Ai Liu, Fang Sun, He Li, Su Wen Lei, Ju Fang Wang
OBJECTIVE: To explore the role of p21 in ionizing radiation-induced changes in protein levels during the G2/M transition and long-term G2 arrest. METHODS: Protein expression levels were assessed by western blot in the human uveal melanoma 92-1 cells after treatment with ionizing radiation. Depletion of p21 was carried out by employing the siRNA technique. Cell cycle distribution was determined by flow cytometry combined with histone H3 phosphorylation at Ser28, an M-phase marker...
July 2016: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/27543214/ki-67-p53-and-p16-expression-and-g691s-ret-polymorphism-in-desmoplastic-melanoma-dm-a-clinicopathologic-analysis-of-predictors-of-outcome
#6
Nicholas F Lawrence, Marc R Hammond, Dennie T Frederick, Yuhua Su, Dora Dias-Santagata, April Deng, M Angelica Selim, Meera Mahalingam, Keith T Flaherty, Mai P Hoang
BACKGROUND: The prognostic role Ki-67, p53, and p16 immunostains and RET (rearranged during transfection) polymorphism in desmoplastic melanoma has not been evaluated. OBJECTIVE: We sought to identify potential prognostic markers. METHODS: We performed Ki-67, p53, and p16 immunostains on 66 desmoplastic melanomas, and sequenced RET G691 polymorphism and recurrent mutations of 17 cancer genes in 55 and 20 cases, respectively. RESULTS: Recurrence and metastasis were documented in 11 of 66 (17%) and 26 of 66 (39%) patients, respectively...
September 2016: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/27272799/diagnostic-and-prognostic-value-of-proex-c-and-glut1-in-melanocytic-lesions
#7
Shaofeng Yan, Bryan N Coffing, Zhongze Li, Haiyi Xie, Jeoffry B Brennick, Haaris A Beg, Heather M Froehlich, Wendy A Wells
BACKGROUND: Melanoma is one of the most aggressive types of skin cancer. The purpose of this study was to evaluate the use of two biomarkers, ProEx C and glucose transporter isoform 1 (GLUT1), in the diagnosis and prognostication of melanoma. MATERIALS AND METHODS: We analyzed 129 melanomas and 59 benign nevi in a tissue microarray using immunohistochemical method with antibodies to topoisomerase IIα (TOP2A) and minichromosome maintenance complex component 2 (MCM2) using ProEx C and to GLUT1...
June 2016: Anticancer Research
https://www.readbyqxmd.com/read/26982777/melanoma-patterns-of-distant-relapse-a-study-of-108-cases-from-a-south-brazilian-center
#8
Rodrigo Kraft Rovere, Maria Eduarda Pires de Souza, Danielle Louise da Maia Cidral, Sara Fernanda Hilgert, Yasmine Rodrigues Chamse Ddine, Carlos Efrain Stein, Giuliano Santos Borges, Adma Silva de Lima
BACKGROUND: The incidence of cutaneous melanoma has increased over the last decades. Recurrences occur most frequently within the first 2-3 years after diagnosis but patients carry a lifelong risk of relapse. Nevertheless, there is no consensus in the literature on what screening tests patients should undergo. OBJECTIVES: To evaluate the most common melanoma metastasis sites among a South Brazilian population from a city with one of the highest melanoma rates, and establish the best screening method for these patients...
January 2016: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/26980024/braf-and-epithelial-mesenchymal-transition-in-primary-cutaneous-melanoma-a-role-for-snail-and-e-cadherin
#9
Brendon Mitchell, Dominick A Leone, John K Feller, Shi Yang, Meera Mahalingam
In vitro studies in melanoma indicate that up-regulation of the transcriptional repressor Snail occurs with a concomitant decrease of its target E-cadherin, both hallmarks of epithelial-mesenchymal transition-an association not established in vivo. We sought to elucidate the relationship between BRAF, Snail, E-cadherin, and established histopathologic prognosticators in primary cutaneous melanoma. Archived annotated samples with a diagnosis of primary cutaneous melanoma were retrieved (n = 68 cases; 34 BRAF mutant and 34 BRAF wild type) and immunohistochemically stained for Snail and E-cadherin protein expression...
June 2016: Human Pathology
https://www.readbyqxmd.com/read/26868000/the-protein-phosphatase-6-catalytic-subunit-ppp6c-is-indispensable-for-proper-post-implantation-embryogenesis
#10
Honami Ogoh, Nobuhiro Tanuma, Yasuhisa Matsui, Natsuki Hayakawa, Ayaka Inagaki, Mami Sumiyoshi, Yuki Momoi, Ayako Kishimoto, Mai Suzuki, Nozomi Sasaki, Tsukasa Ohuchi, Miyuki Nomura, Yuriko Teruya, Keiko Yasuda, Toshio Watanabe, Hiroshi Shima
Ppp6c, which encodes the catalytic subunit of phosphoprotein phosphatase 6 (PP6), is conserved among eukaryotes from yeast to humans. In mammalian cells, PP6 targets IκBε for degradation, activates DNA-dependent protein kinase to trigger DNA repair, and is reportedly required for normal mitosis. Recently, Ppp6c mutations were identified as candidate drivers of melanoma and skin cancer. Nonetheless, little is known about the physiological role of Ppp6c. To investigate this function in vivo, we established mice lacking the Ppp6c phosphatase domain by crossing heterozygous mutants...
February 2016: Mechanisms of Development
https://www.readbyqxmd.com/read/26859408/absence-of-tumor-infiltrating-lymphocyte-is-a-reproducible-predictive-factor-for-sentinel-lymph-node-metastasis-a-multicenter-database-study-by-the-brazilian-melanoma-group
#11
João Pedreira Duprat, Eduard René Brechtbülh, Bianca Costa de Sá, Mauro Enokihara, Jose Humberto Fregnani, Gilles Landman, Marcus Maia, Felice Riccardi, Francisco Alberto Belfort, Alberto Wainstein, Luciana F Moredo, Higino Steck, Miguel Brandão, Marcelo Moreno, Eduardo Miranda, Ivan Dunshee de Oliveira Santos
AIMS: The aim of this study is to confirm the function of tumor-infiltrating lymphocytes (TILs) in sentinel lymph node (SLN) metastasis. MATERIALS AND METHODS: This retrospective study included 633 patients with invasive melanoma who underwent sentinel lymph node biopsy in 7 referral centers certified by the Brazilian Melanoma Group. Independent risk factors of sentinel node metastasis (SNL) were identified by multiple logistic regression. RESULTS: SLN metastasis was detected in 101 of 633 cases (16...
2016: PloS One
https://www.readbyqxmd.com/read/26827186/association-of-sentinel-lymph-node-diameter-with-melanoma-metastasis
#12
Justin Merkow, Alessandro Paniccia, Edward Jones, Teresa Jones, Maggie Hodges, Robert Stovall, Nicole Kounalakis, Csaba Gajdos, Karl Lewis, William Robinson, Rene Gonzalez, Nathan Pearlman, Martin McCarter
BACKGROUND: Lymph nodes are an important part of the immune system and the size of the lymph node reflects local immunologic activity. The purpose of this study was to examine the association between sentinel lymph node (SLN) size and the presence of nodal metastasis in patients with melanoma. METHODS: Retrospective review of a prospectively maintained database of patients undergoing SLN biopsy for cutaneous melanoma between February 1995 and January 2013. The maximum pathologic diameter and the volume of the largest node was used...
August 2016: American Journal of Surgery
https://www.readbyqxmd.com/read/26663842/structure-elucidation-antimicrobial-and-cytotoxic-activities-of-a-halimane-isolated-from-vellozia-kolbekii-alves-velloziaceae
#13
Carmelita G Silva, Helvécio M Santos Júnior, Jussara P Barbosa, Gisela L Costa, Felipe A R Rodrigues, Denilson F Oliveira, Letícia V Costa-Lotufo, Ruy J V Alves, Elis C A Eleutherio, Claudia M Rezende
A new halimane diterpene was isolated from Vellozia kolbekii Alves (Velloziaceae) and identified as (5R,8R,9S,13R)-halim-1,10-ene-15,16-diol (1). It showed cytotoxicity against three human cancer cell lines, SF-295 (glioblastoma), MDA-MB-435 (melanoma), and HCT-8 (colon adenocarcinoma). In the mechanism of cytotoxic action, halimane 1 interferes in two major phases of the cell cycle: in S phase, in which DNA synthesis occurs and where it is very sensitive to damage, and G2M phase which is the phase of preparation for mitosis and mitosis itself, showing apoptosis-inducing properties...
December 2015: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/26542815/a-retrospective-multicenter-analysis-of-the-predictive-value-of-mitotic-rate-for-sentinel-lymph-node-sln-positivity-in-thin-melanomas
#14
MULTICENTER STUDY
Heidi Wat, Ambikaipakan Senthilselvan, Thomas G Salopek
BACKGROUND: There is a paucity of studies to substantiate whether the presence of a single mitosis justifies sentinel lymph node (SLN) biopsy (SLNB) in thin melanomas. OBJECTIVE: We sought to determine if mitotic rate is associated with SLNB outcome when taking into account other prognostic factors. METHODS: All cases of melanoma that underwent SLNB in the province of Alberta, Canada, between 2007 and 2013 were reviewed through a provincial tumor database...
January 2016: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/26521212/vitamin-d-receptor-polymorphisms-and-survival-in-patients-with-cutaneous-melanoma-a-population-based-study
#15
MULTICENTER STUDY
Irene Orlow, Anne S Reiner, Nancy E Thomas, Pampa Roy, Peter A Kanetsky, Li Luo, Susan Paine, Bruce K Armstrong, Anne Kricker, Loraine D Marrett, Stefano Rosso, Roberto Zanetti, Stephen B Gruber, Hoda Anton-Culver, Richard P Gallagher, Terence Dwyer, Klaus Busam, Colin B Begg, Marianne Berwick
Factors known to affect melanoma survival include age at presentation, sex and tumor characteristics. Polymorphisms also appear to modulate survival following diagnosis. Result from other studies suggest that vitamin D receptor (VDR) polymorphisms (SNPs) impact survival in patients with glioma, renal cell carcinoma, lung, breast, prostate and other cancers; however, a comprehensive study of VDR polymorphisms and melanoma-specific survival is lacking. We aimed to investigate whether VDR genetic variation influences survival in patients with cutaneous melanoma...
January 2016: Carcinogenesis
https://www.readbyqxmd.com/read/26504497/a-gene-expression-signature-identifying-transient-dnmt1-depletion-as-a-causal-factor-of-cancer-germline-gene-activation-in-melanoma
#16
Julie Cannuyer, Aurélie Van Tongelen, Axelle Loriot, Charles De Smet
BACKGROUND: Many human tumors show aberrant activation of a group of germline-specific genes, termed cancer-germline (CG) genes, several of which appear to exert oncogenic functions. Although activation of CG genes in tumors has been linked to promoter DNA demethylation, the mechanisms underlying this epigenetic alteration remain unclear. Two main processes have been proposed: awaking of a gametogenic program directing demethylation of target DNA sequences via specific regulators, or general deficiency of DNA methylation activities resulting from mis-targeting or down-regulation of the DNMT1 methyltransferase...
2015: Clinical Epigenetics
https://www.readbyqxmd.com/read/26476911/atypical-spitz-tumor-of-uncertain-biologic-potential-with-inopportune-localization-in-a-7-year-old-boy
#17
LETTER
Nedeljka Glavan, Gordana Zamolo, Miljenko Katunarić, Nives Jonjić
No abstract text is available yet for this article.
2015: Acta Dermatovenerologica Croatica: ADC
https://www.readbyqxmd.com/read/26278815/detection-of-mitotic-figures-in-thin-melanomas-immunohistochemistry-does-not-replace-the-careful-search-for-mitotic-figures-in-hematoxylin-eosin-stain
#18
COMPARATIVE STUDY
Karl Ottmann, Michael Tronnier, Christina Mitteldorf
BACKGROUND: The mitotic rate is an important prognostic criterion in patients with thin melanoma ≤ 1 mm. OBJECTIVE: The aim of this study was to investigate the reproducibility of the mitotic rate in thin melanoma in hematoxylin-eosin (H&E) stain and compare it with the detection of mitotic figures by immunohistochemistry. METHODS: The number of mitoses stated in the routine diagnostic report in 190 pT1 melanomas was compared with the number gained from re-evaluation of H&E sections and the number detected after staining with the mitotic marker, phosphohistone H3 (PHH3)...
October 2015: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/26277601/-update-on-surgical-treatment-of-primary-and-metastatic-cutaneous-melanoma
#19
María Alejandra Zuluaga-Sepúlveda, Ivonne Arellano-Mendoza, Jorge Ocampo-Candiani
Melanoma is a common cutaneous tumour. It is of great importance due to its increasing incidence and aggressive behaviour, with metastasis to lymph nodes and internal organs. When suspecting melanoma, excisional biopsy should be performed to obtain complete histological information in order to determine the adverse factors such as ulceration, mitosis rate, and Breslow depth, which influence preoperative staging and provide data for sentinel lymph biopsy decision making. The indicated management for melanoma is wide local excision, observing recommended and well-established excision margins, depending on Breslow depth and anatomical location of the tumour...
January 2016: Cirugia y Cirujanos
https://www.readbyqxmd.com/read/26274027/psf-sfpq-is-a-very-common-gene-fusion-partner-in-tfe3-rearrangement-associated-perivascular-epithelioid-cell-tumors-pecomas-and-melanotic-xp11-translocation-renal-cancers-clinicopathologic-immunohistochemical-and-molecular-characteristics-suggesting-classification
#20
Qiu Rao, Qin Shen, Qiu-yuan Xia, Zi-yu Wang, Biao Liu, Shan-shan Shi, Qun-li Shi, Hong-lin Yin, Bo Wu, Sheng-bing Ye, Li Li, Jie-Yu Chen, Min-hong Pan, Qing Li, Rui Li, Xuan Wang, Ru-song Zhang, Bo Yu, Heng-hui Ma, Zhen-feng Lu, Xiao-jun Zhou
An increasing number of TFE3 rearrangement-associated tumors, such as TFE3 rearrangement-associated perivascular epithelioid cell tumors (PEComas), melanotic Xp11 translocation renal cancers, and melanotic Xp11 neoplasms, have recently been reported. We examined 12 such cases, including 5 TFE3 rearrangement-associated PEComas located in the pancreas, cervix, or pelvis and 7 melanotic Xp11 translocation renal cancers, using clinicopathologic, immunohistochemical, and molecular analyses. All the tumors shared a similar morphology, including a purely nested or sheet-like architecture separated by a delicate vascular network, purely epithelioid cells displaying a clear or granular eosinophilic cytoplasm, a lack of papillary structures and spindle cell or fat components, uniform round or oval nuclei containing small visible nucleoli, and, in most cases (11/12), melanin pigmentation...
September 2015: American Journal of Surgical Pathology
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