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Drosophila melanogaster insect innate immunity

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https://www.readbyqxmd.com/read/28706521/evolution-and-function-of-thioester-containing-proteins-and-the-complement-system-in-the-innate-immune-response
#1
REVIEW
Upasana Shokal, Ioannis Eleftherianos
The innate immune response is evolutionary conserved among organisms. The complement system forms an important and efficient immune defense mechanism. It consists of plasma proteins that participate in microbial detection, which ultimately results in the production of various molecules with antimicrobial activity. Thioester-containing proteins (TEPs) are a superfamily of secreted effector proteins. In vertebrates, certain TEPs act in the innate immune response by promoting recruitment of immune cells, phagocytosis, and direct lysis of microbial invaders...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28705811/proprotein-convertase-furin1-expression-in-the-drosophila-fat-body-is-essential-for-a-normal-antimicrobial-peptide-response-and-bacterial-host-defense
#2
Saara Aittomäki, Susanna Valanne, Tapio Lehtinen, Sampsa Matikainen, Tuula A Nyman, Mika Rämet, Marko Pesu
Invading pathogens provoke robust innate immune responses in Dipteran insects, such as Drosophila melanogaster In a systemic bacterial infection, a humoral response is induced in the fat body. Gram-positive bacteria trigger the Toll signaling pathway, whereas gram-negative bacterial infections are signaled via the immune deficiency (IMD) pathway. We show here that the RNA interference-mediated silencing of Furin1-a member of the proprotein convertase enzyme family-specifically in the fat body, results in a reduction in the expression of antimicrobial peptides...
July 13, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28656506/comparing-naturally-occurring-glycosylated-forms-of-proline-rich-antibacterial-peptide-drosocin
#3
Deepti S Lele, Gagandeep Kaur, Menithalaxmi Thiruvikraman, Kanwal J Kaur
Antimicrobial peptides (AMPs) are key players of innate immunity. Amongst various classes of AMPs, proline rich AMPs from insects enjoy special attention with few members of this class bearing O-glycosylation as post-translational modification. Drosocin, a 19 amino acid glycosylated AMP is a member of proline rich class, synthesized in the haemolymph of Drosophila melanogaster upon bacterial challenge. We report herein the chemical synthesis of drosocin carrying disaccharide (β-Gal(1 → 3)α-GalNAc) and comparison of its structural and functional properties with another naturally occurring monoglycosylated form of drosocin i...
June 27, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28497117/rna-interference-restricts-rift-valley-fever-virus-in-multiple-insect-systems
#4
Isabelle Dietrich, Stephanie Jansen, Gamou Fall, Stephan Lorenzen, Martin Rudolf, Katrin Huber, Anna Heitmann, Sabine Schicht, El Hadji Ndiaye, Mick Watson, Ilaria Castelli, Benjamin Brennan, Richard M Elliott, Mawlouth Diallo, Amadou A Sall, Anna-Bella Failloux, Esther Schnettler, Alain Kohl, Stefanie C Becker
The emerging bunyavirus Rift Valley fever virus (RVFV) is transmitted to humans and livestock by a large number of mosquito species. RNA interference (RNAi) has been characterized as an important innate immune defense mechanism used by mosquitoes to limit replication of positive-sense RNA flaviviruses and togaviruses; however, little is known about its role against negative-strand RNA viruses such as RVFV. We show that virus-specific small RNAs are produced in infected mosquito cells, in Drosophila melanogaster cells, and, most importantly, also in RVFV vector mosquitoes...
May 2017: MSphere
https://www.readbyqxmd.com/read/28476910/the-immune-phenotype-of-three-drosophila-leukemia-models
#5
Badrul Arefin, Martin Kunc, Robert Krautz, Ulrich Theopold
Many leukemia patients suffer from dysregulation of their immune system, making them more susceptible to infections and leading to general weakening (cachexia). Both adaptive and innate immunity are affected. The fruit fly Drosophila melanogaster has an innate immune system, including cells of the myeloid lineage (hemocytes). To study Drosophila immunity and physiology during leukemia, we established three models by driving expression of a dominant-active version of the Ras oncogene (Ras(V12) ) alone or combined with knockdowns of tumor suppressors in Drosophila hemocytes...
July 5, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28102430/innate-and-intrinsic-antiviral-immunity-in-drosophila
#6
REVIEW
Assel Mussabekova, Laurent Daeffler, Jean-Luc Imler
The fruit fly Drosophila melanogaster has been a valuable model to investigate the genetic mechanisms of innate immunity. Initially focused on the resistance to bacteria and fungi, these studies have been extended to include antiviral immunity over the last decade. Like all living organisms, insects are continually exposed to viruses and have developed efficient defense mechanisms. We review here our current understanding on antiviral host defense in fruit flies. A major antiviral defense in Drosophila is RNA interference, in particular the small interfering (si) RNA pathway...
June 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/27932997/characterization-of-reproductive-dormancy-in-male-drosophila-melanogaster
#7
Olga I Kubrak, Lucie Kučerová, Ulrich Theopold, Sören Nylin, Dick R Nässel
Insects are known to respond to seasonal and adverse environmental changes by entering dormancy, also known as diapause. In some insect species, including Drosophila melanogaster, dormancy occurs in the adult organism and postpones reproduction. This adult dormancy has been studied in female flies where it is characterized by arrested development of ovaries, altered nutrient stores, lowered metabolism, increased stress and immune resistance and drastically extended lifespan. Male dormancy, however, has not been investigated in D...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27811341/the-insect-derived-antimicrobial-peptide-metchnikowin-targets-fusarium-graminearum-%C3%AE-1-3-glucanosyltransferase-gel1-which-is-required-for-the-maintenance-of-cell-wall-integrity
#8
Mohammad-Reza Bolouri Moghaddam, Andreas Vilcinskas, Mohammad Rahnamaeian
Antimicrobial peptides (AMPs) are essential components of the insect innate immune system. Their diversity provides protection against a broad spectrum of microbes and they have several distinct modes of action. Insect-derived AMPs are currently being developed for both medical and agricultural applications, and their expression in transgenic crops confers resistance against numerous plant pathogens. The antifungal peptide metchnikowin (Mtk), which was originally discovered in the fruit fly Drosophila melanogaster, is of particular interest because it has potent activity against economically important phytopathogenic fungi of the phylum Ascomycota, such as Fusarium graminearum, but it does not harm beneficial fungi such as the mycorrhizal basidiomycete Piriformospora indica...
April 1, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/27317784/variation-and-evolution-in-the-glutamine-rich-repeat-region-of-drosophila-argonaute-2
#9
William H Palmer, Darren J Obbard
RNA interference pathways mediate biological processes through Argonaute-family proteins, which bind small RNAs as guides to silence complementary target nucleic acids . In insects and crustaceans Argonaute-2 silences viral nucleic acids, and therefore acts as a primary effector of innate antiviral immunity. Although the function of the major Argonaute-2 domains, which are conserved across most Argonaute-family proteins, are known, many invertebrate Argonaute-2 homologs contain a glutamine-rich repeat (GRR) region of unknown function at the N-terminus ...
2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/26847920/co-expression-of-dorsal-and-rel2-negatively-regulates-antimicrobial-peptide-expression-in-the-tobacco-hornworm-manduca-sexta
#10
Xue Zhong, Xiang-Jun Rao, Hui-Yu Yi, Xin-Yu Lin, Xiao-Hong Huang, Xiao-Qiang Yu
Nuclear factor κB (NF-κB) plays an essential role in regulation of innate immunity. In mammals, NF-κB factors can form homodimers and heterodimers to activate gene expression. In insects, three NF-κB factors, Dorsal, Dif and Relish, have been identified to activate antimicrobial peptide (AMP) gene expression. However, it is not clear whether Dorsal (or Dif) and Relish can form heterodimers. Here we report the identification and functional analysis of a Dorsal homologue (MsDorsal) and two Relish short isoforms (MsRel2A and MsRel2B) from the tobacco hornworm, Manduca sexta...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26847724/immunity-of-the-greater-wax-moth-galleria-mellonella
#11
REVIEW
Iwona Wojda
Investigation of insect immune mechanisms provides important information concerning innate immunity, which in many aspects is conserved in animals. This is one of the reasons why insects serve as model organisms to study virulence mechanisms of human pathogens. From the evolutionary point of view, we also learn a lot about host-pathogen interaction and adaptation of organisms to conditions of life. Additionally, insect-derived antibacterial and antifungal peptides and proteins are considered for their potential to be applied as alternatives to antibiotics...
June 2017: Insect Science
https://www.readbyqxmd.com/read/26653876/genome-wide-identification-and-expression-profiling-of-serine-proteases-and-homologs-in-the-diamondback-moth-plutella-xylostella-l
#12
Hailan Lin, Xiaofeng Xia, Liying Yu, Liette Vasseur, Geoff M Gurr, Fengluan Yao, Guang Yang, Minsheng You
BACKGROUND: Serine proteases (SPs) are crucial proteolytic enzymes responsible for digestion and other processes including signal transduction and immune responses in insects. Serine protease homologs (SPHs) lack catalytic activity but are involved in innate immunity. This study presents a genome-wide investigation of SPs and SPHs in the diamondback moth, Plutella xylostella (L.), a globally-distributed destructive pest of cruciferous crops. RESULTS: A total of 120 putative SPs and 101 putative SPHs were identified in the P...
December 10, 2015: BMC Genomics
https://www.readbyqxmd.com/read/26546607/protection-of-insects-against-viral-infection-by-apoptosis-dependent-phagocytosis
#13
Firzan Nainu, Yumiko Tanaka, Akiko Shiratsuchi, Yoshinobu Nakanishi
We investigated whether phagocytosis participates in the protection of insects from viral infection using the natural host-virus interaction between Drosophila melanogaster and Drosophila C virus (DCV). Drosophila S2 cells were induced to undergo apoptotic cell death upon DCV infection. However, UV-inactivated virus was unable to cause apoptosis, indicating the need for productive infection for apoptosis induction. S2 cells became susceptible to phagocytosis by hemocyte-derived l(2)mbn cells after viral infection, and the presence of phagocytes in S2 cell cultures reduced viral proliferation...
December 15, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/26437768/drosophila-innate-immunity-regional-and-functional-specialization-of-prophenoloxidases
#14
Jan P Dudzic, Shu Kondo, Ryu Ueda, Casey M Bergman, Bruno Lemaitre
BACKGROUND: The diversification of immune systems during evolution involves the expansion of particular gene families in given phyla. A better understanding of the metazoan immune system requires an analysis of the logic underlying such immune gene amplification. This analysis is now within reach due to the ease with which we can generate multiple mutations in an organism. In this paper, we analyze the contribution of the three Drosophila prophenoloxidases (PPOs) to host defense by generating single, double and triple mutants...
2015: BMC Biology
https://www.readbyqxmd.com/read/26198742/scrutinizing-the-immune-defence-inventory-of-camponotus-floridanus-applying-total-transcriptome-sequencing
#15
Shishir K Gupta, Maria Kupper, Carolin Ratzka, Heike Feldhaar, Andreas Vilcinskas, Roy Gross, Thomas Dandekar, Frank Förster
BACKGROUND: Defence mechanisms of organisms are shaped by their lifestyle, environment and pathogen pressure. Carpenter ants are social insects which live in huge colonies comprising genetically closely related individuals in high densities within nests. This lifestyle potentially facilitates the rapid spread of pathogens between individuals. In concert with their innate immune system, social insects may apply external immune defences to manipulate the microbial community among individuals and within nests...
2015: BMC Genomics
https://www.readbyqxmd.com/read/26177454/resistance-to-innate-immunity-contributes-to-colonization-of-the-insect-gut-by-yersinia-pestis
#16
Shaun C Earl, Miles T Rogers, Jennifer Keen, David M Bland, Andrew S Houppert, Caitlynn Miller, Ian Temple, Deborah M Anderson, Melanie M Marketon
Yersinia pestis, the causative agent of bubonic and pneumonic plague, is typically a zoonotic vector-borne disease of wild rodents. Bacterial biofilm formation in the proventriculus of the flea contributes to chronic infection of fleas and facilitates efficient disease transmission. However prior to biofilm formation, ingested bacteria must survive within the flea midgut, and yet little is known about vector-pathogen interactions that are required for flea gut colonization. Here we establish a Drosophila melanogaster model system to gain insight into Y...
2015: PloS One
https://www.readbyqxmd.com/read/25992475/systemic-bacterial-infection-and-immune-defense-phenotypes-in-drosophila-melanogaster
#17
Sarah Khalil, Eliana Jacobson, Moria C Chambers, Brian P Lazzaro
The fruit fly Drosophila melanogaster is one of the premier model organisms for studying the function and evolution of immune defense. Many aspects of innate immunity are conserved between insects and mammals, and since Drosophila can readily be genetically and experimentally manipulated, they are powerful for studying immune system function and the physiological consequences of disease. The procedure demonstrated here allows infection of flies by introduction of bacteria directly into the body cavity, bypassing epithelial barriers and more passive forms of defense and allowing focus on systemic infection...
2015: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/25979357/parkin-mediated-responses-against-infection-and-wound-involve-tspo-vdac-complex-in-drosophila
#18
Jae Ho Cho, Jeong Hyang Park, Chang Geon Chung, Hyun-Jung Shim, Keun Hye Jeon, Seong-Woon Yu, Sung Bae Lee
Parkin, an E3 ubuquitin ligase associated with Parkinson's disease (PD), has recently been implicated in mediating innate immunity. However, molecular details regarding parkin-mediated immune response remain to be elucidated. Here, we identified mitochondrial TSPO-VDAC complex to genetically interact with parkin in mediating responses against infection and wound in Drosophila. The loss-of-function mutation in parkin results in defective immune response against bacterial infection. Additionally, parkin mutant larvae showed hypersensitivity against wound regardless of bacterial infection...
July 17, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25797797/peptidoglycan-recognition-protein-s2-from-silkworm-integument-characterization-microbe-induced-expression-and-involvement-in-the-immune-deficiency-pathway
#19
Jie Yang, Xiaonan Wang, Shunming Tang, Zhongyuan Shen, Jinmei Wu
Peptidoglycan recognition protein (PGRP) binds specifically to peptidoglycan and plays an important role as a pattern recognition receptor in the innate immunity of insects. The cDNA of a short-type PGRP, an open reading frame of 588 bp encoding a polypeptide of 196 amino acids, was cloned from Bombyx mori. A phylogenetic tree was constructed, and the results showed that BmPGRP-S2 was most similar to Drosophila melanogaster PGRP (DmPGRP-SA). The induced expression profile of BmPGRP-S2 in healthy Escherichia coli- and Bacillus subtilis-challenged B...
2015: Journal of Insect Science
https://www.readbyqxmd.com/read/25770426/dip1-plays-an-antiviral-role-against-dcv-infection-in-drosophila-melanogaster
#20
Qi Zhang, Liqin Zhang, Xinlei Gao, Shuishui Qi, Zhaoxia Chang, Qingfa Wu
Disconnected Interacting Protein 1 (DIP1) is a dsRNA-binding protein that participates in a wide range of cellular processes. Whether DIP1 is involved in innate immunity remains unclear. Here, DIP1 was found to play an antiviral role in S2 cells. Its antiviral action is specific for DCV infection and not for DXV infection. dip1 mutant flies are hypersensitive to DCV infection. The increased mortality in dip1 mutant flies is associated with the accumulation of DCV positive-stranded RNAs in vivo. This study demonstrated that dip1 is a novel antiviral gene that restricts DCV replication in vitro and in vivo...
May 1, 2015: Biochemical and Biophysical Research Communications
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