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yongping huang

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https://www.readbyqxmd.com/read/28271691/comparative-analysis-of-serum-allergen-sige-detected-by-western-blotting-and-fluorescent-enzyme-immunoassay
#1
Xi Chen, Peiyan Zheng, Nili Wei, Huimin Huang, Wenting Luo, Yongping Lin, Baoqing Sun
BACKGROUND: Various new methods to diagnose allergens have gradually developed in recent years. The present study aimed to evaluate the diagnostic performance and clinical utility of Allergy-Q detection system (Proteometech Inc., Korea) on serum allergen-specific IgE (sIgE). METHODS: Immunoblotting (Allergy-Q system) and fluorescent enzyme immunoassay (ImmunoCAP system, Phadia Inc., Sweden) were used to detect serum total IgE and allergen sIgE of 244 cases of allergic diseases, followed by comparative analysis of the results...
March 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28187533/comparison-of-analytic-performances-of-cellsearch-and-ifish-approach-in-detecting-circulating-tumor-cells
#2
Yuan Sheng, Ting Wang, Hengyu Li, Zhenzhen Zhang, Jianghao Chen, Chenyang He, Yongping Li, Yonggang Lv, Juliang Zhang, Cheng Xu, Zhen Wang, Chen Huang, Ling Wang
Circulating tumor cells (CTCs) have been widely used to predict the prognosis of breast cancer patients. The aim of the present study was to compare the performances of Cellsearch and immunostaining-fluorescence in situ hybridization (iFISH) in detecting CTCs in breast cancer patients. Forty-five newly diagnosed breast cancer patients and 14 healthy donors were recruited and their CTCs were detected by both Cellsearch and iFISH. Correlation between clinicopathological features and CTCs was investigated. We found that the positive rate of CTC detected by iFISH was significantly higher than by Cellsearch system (91% vs 38%)...
January 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28127609/-installation-art-like-hierarchical-self-assembly-of-giant-polymeric-elliptical-platelets
#3
Lei Huang, Zuotao Lei, Tong Huang, Yongfeng Zhou, Yongping Bai
This paper reports the aqueous self-assembly of giant elliptical platelets over 20 μm in axial length, from a novel polyamide. Both the self-assembly pathway and mechanism were studied using morphology and X-ray characterizations. The polymer first self-organizes into small quadrangular frustum pyramid platelets, and then these small platelets can be further installed into giant elliptical platelets through an "installation art"-like hierarchical self-assembly process driven by crystallization. The as-prepared regular giant platelets can further aggregate together into multi-horned or flower-like superstructures...
January 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28122981/transgenic-crispr-cas9-mediated-viral-gene-targeting-for-antiviral-therapy-of-bombyx-mori-nucleopolyhedrovirus-bmnpv
#4
Shuqing Chen, Chengxiang Hou, Honglun Bi, Yueqiang Wang, Jun Xu, Muwang Li, Anthony A James, Yongping Huang, Anjiang Tan
We developed a novel anti-viral strategy by combining transposon-based transgenesis and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system for direct cleavage of the Bombyx mori nucleopolyhedrovirus (BmNPV) genome DNA to promote virus clearance in silkworms. We demonstrate that transgenic silkworms constitutively-expressing Cas9 and guide RNAs (gRNAs) targeting the BmNPV immediate early-1 (ie-1) gene and me53 gene effectively induce target-specific cleavage and subsequent mutagenesis, especially large segment deletions (∼ 7 kilobase-pairs [kb]) in BmNPV genomes and thus exhibit a robust suppression of BmNPV proliferation...
January 25, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28103247/bombyx-mori-p-element-somatic-inhibitor-bmpsi-is-a-key-auxiliary-factor-for-silkworm-male-sex-determination
#5
Jun Xu, Shuqing Chen, Baosheng Zeng, Anthony A James, Anjiang Tan, Yongping Huang
Manipulation of sex determination pathways in insects provides the basis for a wide spectrum of strategies to benefit agriculture and public health. Furthermore, insects display a remarkable diversity in the genetic pathways that lead to sex differentiation. The silkworm, Bombyx mori, has been cultivated by humans as a beneficial insect for over two millennia, and more recently as a model system for studying lepidopteran genetics and development. Previous studies have identified the B. mori Fem piRNA as the primary female determining factor and BmMasc as its downstream target, while the genetic scenario for male sex determination was still unclear...
January 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28057597/depletion-of-juvenile-hormone-esterase-extends-larval-growth-in-bombyx-mori
#6
Zhongjie Zhang, Xiaojing Liu, Takahiro Shiotsuki, Zhisheng Wang, Xia Xu, Yongping Huang, Muwang Li, Kai Li, Anjiang Tan
Two major hormones, juvenile hormone (JH) and 20-hydroxyecdysone (20E), regulate insect growth and development according to their precisely coordinated titres, which are controlled by both biosynthesis and degradation pathways. Juvenile hormone esterase (JHE) is the primary JH-specific degradation enzyme that plays a key role in regulating JH titers, along with JH epoxide hydrolase (JHEH) and JH diol kinase (JHDK). In the current study, a loss-of-function analysis of JHE in the silkworm, Bombyx mori, was performed by targeted gene disruption using the transgenic CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/RNA-guided Cas9 nucleases) system...
February 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28039161/the-different-t-cell-receptor-repertoires-in-breast-cancer-tumors-draining-lymph-nodes-and-adjacent-tissues
#7
Ting Wang, Changxi Wang, Jinghua Wu, Chenyang He, Wei Zhang, Jiayun Liu, Ruifang Zhang, Yonggang Lv, Yongping Li, Xiaojing Zeng, Hongzhi Cao, Xiuqing Zhang, Xun Xu, Chen Huang, Ling Wang, Xiao Liu
T lymphocytes infiltrate the microenvironment of breast cancer tumors and play a pivotal role in tumor immune surveillance. Relationships between the T-cell receptors (TCR) borne by T cells within tumors, in the surrounding tissues, and in draining lymph nodes are largely unexplored in human breast cancer. Consequently, information about the relative extent of possible T-cell exchange between these tissues is also lacking. Here, we have analyzed the TCR repertoire of T cells using multiplex PCR and high-throughput sequencing of the TCRβ chain in the tissues of tumor, adjacent nontumor, and axillary lymph nodes of breast cancer patients...
February 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/27998055/pillared-structure-design-of-mxene-with-ultra-large-interlayer-spacing-for-high-performance-lithium-ion-capacitors
#8
Jianmin Luo, Wenkui Zhang, Huadong Yuan, Chengbin Jin, Liyuan Zhang, Hui Huang, Chu Liang, Yang Xia, Jun Zhang, Yongping Gan, Xinyong Tao
Two-dimensional transition metal carbide materials (termed MXene) have attracted huge attention in the field of electrochemical energy storage due to their excellent electrical conductivity, high volumetric capacity, etc. Herein, with inspiration from the interesting structure of pillared interlayered clays, we attempt to fabricate pillared Ti3C2 MXene (CTAB-Sn(IV)@Ti3C2) via a facile liquid-phase cetyltrimethylammonium bromide (CTAB) pre-pillaring and Sn4+ pillaring method. The interlayer spacing of Ti3C2 MXene can be controlled according to the size of intercalated pre-pillaring agent (cationic surfactant) and can reach 2...
December 21, 2016: ACS Nano
https://www.readbyqxmd.com/read/27989760/mir-200a-acts-as-an-oncogene-in-colorectal-carcinoma-by-targeting-pten
#9
Yijun Li, Jinglu Sun, Yongping Cai, Yan Jiang, Xiaoqing Wang, Xiaojuan Huang, Yu Yin, Hao Li
The expression pattern of miR-200a in different types of cancer is diverse, and its mechanism in tumorigenesis has yet to be elucidated. In this study, miR-200a was significantly upregulated in the cancer tumor tissues of colorectal cancer (CRC) patients, and its expression was positively correlated with the degree of tumor differentiation. Overexpression of miR-200a enhanced cell proliferation, migration, and invasion. To understand the potential mechanism of miR-200a in tumorigenesis, we showed that miR-200a directly targeted phosphatase and tensin homolog (PTEN)...
October 27, 2016: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/27983967/mir-200a-acts-as-an-oncogene-in-colorectal-carcinoma-by-targeting-pten
#10
Yijun Li, Jinglu Sun, Yongping Cai, Yan Jiang, Xiaoqing Wang, Xiaojuan Huang, Yu Yin, Hao Li
The expression pattern of miR-200a in different types of cancer is diverse, and its mechanism in tumorigenesis has yet to be elucidated. In this study, miR-200a was significantly upregulated in the cancer tumor tissues of colorectal cancer (CRC) patients, and its expression was positively correlated with the degree of tumor differentiation. Overexpression of miR-200a enhanced cell proliferation, migration, and invasion. To understand the potential mechanism of miR-200a in tumorigenesis, we showed that miR-200a directly targeted phosphatase and tensin homolog (PTEN)...
December 2016: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/27940541/insecticidal-specificity-of-cry1ah-to-helicoverpa-armigera-is-determined-by-binding-of-apn1-via-domain-ii-loops-2-and-3
#11
Zishan Zhou, Yuxiao Liu, Gemei Liang, Yongping Huang, Alejandra Bravo, Mario Soberón, Fuping Song, Xueping Zhou, Jie Zhang
Bacillus thuringiensis Cry1Ah protein is highly toxic against Helicoverpa armigera but shows no toxicity against Bombyx mori larvae. In contrast, the closely related Cry1Ai toxin showed the opposite phenotype: high activity against B. mori but no toxicity against H. armigera. Analysis of binding of Cry1Ah to brush border membrane vesicle (BBMV) proteins from H. armigera and B. mori by surface plasmon resonance revealed association of toxin binding with insect specificity. Pulldown experiments identified aminopeptidase N1 (APN1) as a Cry1Ah binding protein that was not observed in the assays using B...
February 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27939158/the-arca-gene-contributes-to-the-serum-resistance-and-virulence-of-haemophilus-parasuis-serovar-13-clinical-strain-ep3
#12
Lingqiang Ding, Xintian Wen, Lvqin He, Xuefeng Yan, Yongping Wen, Sanjie Cao, Xiaobo Huang, Rui Wu, Yiping Wen
As a global transcriptional factor, ArcA regulates the expression of hundreds of genes involved in aerobic and anaerobic metabolism. Here we deleted arcA gene and investigated the biological characteristics of arcA deletion mutant (ΔarcA) in Haemophilus parasuis (H. parasuis) serovar 13 clinical strain EP3. Results indicated that deletion of arcA impaired growth of EP3 strain under anaerobic condition, and reduced virulence of EP3 strain in mice. Additionally, the ΔarcA strain showed greater sensitivity in porcine serum and produced less biofilm mass than the EP3 strain...
November 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27917903/global-dna-methylation-variations-after-short-term-heat-shock-treatment-in-cultured-microspores-of-brassica-napus-cv-topas
#13
Jun Li, Qian Huang, Mengxiang Sun, Tianyao Zhang, Hao Li, Biyun Chen, Kun Xu, Guizhen Gao, Feng Li, Guixin Yan, Jiangwei Qiao, Yongping Cai, Xiaoming Wu
Heat stress can induce the cultured microspores into embryogenesis. In this study, whole genome bisulphite sequencing was employed to study global DNA methylation variations after short-term heat shock (STHS) treatments in cultured microspores of Brassica napus cv. Topas. Our results indicated that treatment on cultured Topas microspores at 32 °C for 6 h triggered DNA hypomethylation, particularly in the CG and CHG contexts. And the total number of T32 (Topas 32 °C for 6 h) vs. T0 (Topas 0 h) differentially methylated region-related genes (DRGs) was approximately two-fold higher than that of T18 (Topas 18 °C for 6 h) vs...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27871022/vascular-targeted-photodynamic-therapy-of-gastric-antral-vascular-ectasia-gave
#14
Haixia Qiu, Yongping Mao, Jing Zeng, Ying Wang, Jiaying Zhang, Naiyang Huang, Qingsen Liu, Yunsheng Yang, Enqiang Linghu, Ying Gu
BACKGROUND AND STUDY AIM: Vascular-targeted photodynamic therapy (V-PDT) has been used for several benign vascular diseases. The aim of this pilot study was to demonstrate the potential benefits of VPDT in the treatment of gastric antral vascular ectasia (GAVE). PATIENTS AND METHODS: Data from patients with GAVE (n=5) who underwent endoscopic V-PDT were analyzed retrospectively. Pre- and post-V-PDT clinical and endoscopic features, hemoglobin levels, and transfusion requirement were compared...
January 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27867075/sexually-dimorphic-traits-in-the-silkworm-bombyx-mori-are-regulated-by-doublesex
#15
Jun Xu, Shuai Zhan, Shuqing Chen, Baosheng Zeng, Zhiqian Li, Anthony A James, Anjiang Tan, Yongping Huang
The DM domain genes, doublesex (dsx) in insects, or their structural homologs, male abnormal 3 (mab-3) in nematodes and Dmrt1 (doublesex and mab-3-related transcription factor 1) in mammals, are downstream regulators of the sex determination pathway that control sexually dimorphic development. Despite the functional importance of dsx and its potential applications in sterile insect technologies (SITs), the mechanisms by which it controls sexually dimorphic traits and the subsequent developmental gene networks in insects are poorly understood...
January 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27803977/the-china-mental-health-survey-ii-design-and-field-procedures
#16
Zhaorui Liu, Yueqin Huang, Ping Lv, Tingting Zhang, Hong Wang, Qiang Li, Jie Yan, Yaqin Yu, Changgui Kou, Xiufeng Xu, Jin Lu, Zhizhong Wang, Hongyan Qiu, Yifeng Xu, Yanling He, Tao Li, Wanjun Guo, Hongjun Tian, Guangming Xu, Xiangdong Xu, Yanjuan Ma, Linhong Wang, Limin Wang, Yongping Yan, Bo Wang, Shuiyuan Xiao, Liang Zhou, Lingjiang Li, Liwen Tan, Hongguang Chen, Chao Ma
China Mental Health Survey (CMHS), which was carried out from July 2013 to March 2015, was the first national representative community survey of mental disorders and mental health services in China using computer-assisted personal interview (CAPI). Face-to-face interviews were finished in the homes of respondents who were selected from a nationally representative multi-stage disproportionate stratified sampling procedure. Sample selection was integrated with the National Chronic Disease and Risk Factor Surveillance Survey administered by the National Centre for Chronic and Non-communicable Disease Control and Prevention in 2013, which made it possible to obtain both physical and mental health information of Chinese community population...
November 1, 2016: Social Psychiatry and Psychiatric Epidemiology
https://www.readbyqxmd.com/read/27796403/the-china-mental-health-survey-cmhs-i-background-aims-and-measures
#17
Yueqin Huang, Zhaorui Liu, Hong Wang, Xing Guan, Hongguang Chen, Chao Ma, Qiang Li, Jie Yan, Yaqin Yu, Changgui Kou, Xiufeng Xu, Jin Lu, Zhizhong Wang, Lan Liu, Yifeng Xu, Yanling He, Tao Li, Wanjun Guo, Hongjun Tian, Guangming Xu, Xiangdong Xu, Shuyun Lv, Linhong Wang, Limin Wang, Yongping Yan, Bo Wang, Shuiyuan Xiao, Liang Zhou, Lingjiang Li, Liwen Tan
The China Mental Health Survey (CMHS) is the first nationally representative community survey on mental disorders and mental health services in China. One-step diagnoses for mood disorders, anxiety disorders and substance use disorders were obtained using the Composite International Diagnostic Interview-3.0 (CIDI-3.0), according to the criteria and definition of the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV). A two-step procedure was applied for schizophrenia and other psychotic disorders, using psychosis screening section in CIDI-3...
October 31, 2016: Social Psychiatry and Psychiatric Epidemiology
https://www.readbyqxmd.com/read/27780040/functional-coordination-of-wave-and-wasp-in-c-%C3%A2-elegans-neuroblast-migration
#18
Zhiwen Zhu, Yongping Chai, Yuxiang Jiang, Wenjing Li, Huifang Hu, Wei Li, Jia-Wei Wu, Zhi-Xin Wang, Shanjin Huang, Guangshuo Ou
Directional cell migration is critical for metazoan development. We define two molecular pathways that activate the Arp2/3 complex during neuroblast migration in Caenorhabditis elegans. The transmembrane protein MIG-13/Lrp12 is linked to the Arp2/3 nucleation-promoting factors WAVE or WASP through direct interactions with ABL-1 or SEM-5/Grb2, respectively. WAVE mutations partially impaired F-actin organization and decelerated cell migration, and WASP mutations did not inhibit cell migration but enhanced migration defects in WAVE-deficient cells...
October 24, 2016: Developmental Cell
https://www.readbyqxmd.com/read/27746496/reconstruction-of-kuwada-grade-iv-chronic-achilles-tendon-rupture-by-minimally-invasive-technique
#19
Xudong Miao, Yongping Wu, Huimin Tao, Disheng Yang, Lu Huang
BACKGROUND: Transfer of a flexor hallucis longus (FHL) tendon can not only reconstruct the Achilles tendon but also provide ischemic tendinous tissues with a rich blood supply to enhance wound healing. This retrospective study aims to investigate clinical outcomes in patients who underwent repair of Kuwada grade IV chronic Achilles tendon rupture with long hallucis longus tendons harvested using a minimally invasive technique. MATERIALS AND METHODS: 35 patients who were treated for Kuwada grade IV Achilles tendon injuries from July 2006 to June 2011 were included in this retrospective study...
September 2016: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/27442652/case-report-of-a-li-fraumeni-syndrome-like-phenotype-with-a-de-novo-mutation-in-chek2
#20
Xuehan Zhuang, Yongping Li, Hongzhi Cao, Ting Wang, Jianghao Chen, Jiayun Liu, Liya Lin, Rui Ye, Xinyang Li, Shuang Liu, Weiyang Li, Yonggang Lv, Juliang Zhang, Chenyang He, Xun Xu, Zhen Wang, Chen Huang, Xiao Liu, Ling Wang
BACKGROUND: Cases of multiple tumors are rarely reported in China. In our study, a 57-year-old female patient had concurrent squamous cell carcinoma, mucoepidermoid carcinoma, brain cancer, bone cancer, and thyroid cancer, which has rarely been reported to date. METHODS: To determine the relationship among these multiple cancers, available DNA samples from the thyroid, lung, and skin tumors and from normal thyroid tissue were sequenced using whole exome sequencing...
July 2016: Medicine (Baltimore)
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