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https://www.readbyqxmd.com/read/29236750/identification-of-gad65-aa-114-122-reactive-memory-like-nk-cells-in-newly-diagnosed-type-1-diabetic-patients-by-hla-class-i-pentamers
#1
Valentina Perri, Elena Gianchecchi, Loredana Cifaldi, Marsha Pellegrino, Ezio Giorda, Marco Andreani, Marco Cappa, Alessandra Fierabracci
Type 1 diabetes is an autoimmune disease, in which pancreatic β cells are destroyed by autoreactive T cells in genetically predisposed individuals. Serum beta cell autoantibody specificities have represented the mainstay for classifying diabetes as autoimmune-mediated and for stratifying risk in first-degree relatives. In recent years, approaches were attempted to solve the difficult issue of detecting rare antigen-specific autoreactive T cells and their significance to etiopathogenesis such as the use of the MHC multimer technology...
2017: PloS One
https://www.readbyqxmd.com/read/29224003/human-leukocyte-antigen-g-inhibits-the-anti-tumor-effect-of-natural-killer-cells-via-immunoglobulin-like-transcript-2-in-gastric-cancer
#2
Rui Wan, Zi-Wei Wang, Hui Li, Xu-Dong Peng, Guang-Yi Liu, Jun-Ming Ou, An-Qi Cheng
BACKGROUND/AIMS: Human leukocyte antigen-G (HLA-G) plays an important role in inhibiting natural killer (NK) cell function and promoting immune escape. However, the specific mechanism of HLA-G on NK in gastric cancer (GC) remains not well understood. This study investigated the expression of HLA-G in GC and the role of HLA-G-effected NK cells in GC progression. METHODS: HLA-G expression in GC tissues obtained from 49 patients with GC was analyzed by immunohistochemistry and western blot...
December 7, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29198970/the-choriocarcinoma-cell-line-jeg-3-upregulates-regulatory-t-cell-phenotypes-and-modulates-pro-inflammatory-cytokines-through-hla-g
#3
Wenna Nascimento Melsted, Sara Hyldig Matzen, Mads Hald Andersen, Thomas Vauvert F Hviid
An understanding of the interactions between immune cells and trophoblast cells, as well as choriocarcinoma cells, are of extreme importance in reproductive immunology and cancer immunology. In this study, we found that the human HLA-G-positive choriocarcinoma cell line JEG-3 upregulates CD4+CD25hiCD127lo T cells, increases the expression of HLA-G+CD4+ and CD8+ T cells, and decreases the expression of ILT2+ on CD4+ T cells in resting PBMCs after six days of co-culture. Expression of HLA-G on JEG-3 cells did not affect regulatory T cell phenotypes, but promoted modulation of pro-inflammatory cytokines IFN-γ, TNF-α and IL-17A...
November 30, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/29123965/ig-like-transcript-2-ilt2-suppresses-t-cell-function-in-chronic-lymphocytic-leukemia
#4
Mónica Villa-Álvarez, Seila Lorenzo-Herrero, Ana P Gonzalez-Rodriguez, Alejandro López-Soto, Angel R Payer, Esther Gonzalez-Garcia, Leticia Huergo-Zapico, Segundo Gonzalez
Chronic lymphocytic leukemia (CLL) is associated with a profound dysregulation of the immune system. Loss of T cell function is frequently caused in cancer by sustained signaling of inhibitory receptors. Here, we analyzed the role of the novel inhibitory receptor Ig-like transcript 2 (ILT2) in the pathogenesis of CLL. We observed that ILT2 expression was markedly reduced on leukemic cells, whereas it was increased on CD8 and CD4 T cells from CLL patients, particularly in those patients harboring chromosome 11q deletion, which includes the ATM gene...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/29058308/analysis-of-the-regulatory-function-of-nk-cells-from-patients-with-systemic-lupus-erythematosus
#5
Daniela De Jesús Cruz-González, Diana Gómez-Martin, Lourdes Baranda, Carlos Abud-Mendoza, Jorge Alcocer-Varela, Roberto González-Amaro, Adriana E Monsiváis-Urenda
Natural Killer cells (NK cells) participates in the regulation of the immune response. However, the immunomodulatory function of NK cells in systemic lupus erythematosus (SLE) is not well understood. The aim of this study was to evaluate the regulatory function of NK cells in SLE patients and to identify the NK cells involved in the pathogenesis of this complex disease. We analyzed the expression of NK receptors and costimulatory molecules in peripheral NK cells (CD3-CD56+) from SLE patients as well as the numbers of HLA-DR/CD11c+ NK cells...
October 23, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28932645/intratumor-heterogeneity-of-immune-checkpoints-in-primary-renal-cell-cancer-focus-on-hla-g-ilt2-ilt4
#6
Nathalie Rouas-Freiss, Joel LeMaoult, Jérôme Verine, Diana Tronik-Le Roux, Stéphane Culine, Christophe Hennequin, François Desgrandchamps, Edgardo D Carosella
The establishment and maintenance of anti-tumor immune responses are the objectives of cancer immunotherapy. Despite recent promising advances, the effectiveness of these approaches has been limited by the multiple immunosuppressive mechanisms developed by tumors (checkpoint). The aim of the present study was to demonstrate intratumor heterogeneity at the levels of immune escape strategies and tumor-host relationships. We focused on well-known checkpoints such as PD1/PDL1 and on a new checkpoint involving HLA-G and its receptors ILT2/ILT4...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28767147/programmed-death-1-is-highly-expressed-on-cd8-cd57-t-cells-in-patients-with-stable-multiple-sclerosis-and-inhibits-their-cytotoxic-response-to-epstein-barr-virus
#7
Maria T Cencioni, Roberta Magliozzi, Richard Nicholas, Rehiana Ali, Omar Malik, Richard Reynolds, Giovanna Borsellino, Luca Battistini, Paolo A Muraro
Growing evidence points to a deregulated response to Epstein-Barr virus (EBV) in the central nervous system of patients with multiple sclerosis (MS) as a possible cause of disease. We have investigated the response of a subpopulation of effector CD8+ T cells to EBV in 36 healthy donors and in 35 patients with MS in active and inactive disease. We have measured the expression of markers of degranulation, the release of cytokines, cytotoxicity and the regulation of effector functions by inhibitory receptors, such as programmed death 1 (PD-1) and human inhibitor receptor immunoglobulin-like transcript 2 (ILT2)...
December 2017: Immunology
https://www.readbyqxmd.com/read/28523429/kir-lilrb-and-their-ligands-genes-as-potential-biomarkers-in-recurrent-implantation-failure
#8
REVIEW
Izabela Nowak, Karolina Wilczyńska, Jacek R Wilczyński, Andrzej Malinowski, Paweł Radwan, Michał Radwan, Piotr Kuśnierczyk
Reproductive failure in humans is a very important social and economic problem, because nowadays women decide to conceive later in life and delay motherhood. Unfortunately, with increasing age they have less chance for natural fertilization and maintenance of pregnancy. Many of them need assisted reproductive technology. Approximately 10% of women after in vitro fertilization-embryo transfers experience recurrent implantation failure (RIF). Multiple factors may contribute to RIF, including oocyte and sperm quality, parental chromosomal anomalies, genetic or metabolic abnormalities of the embryo, poor uterine receptivity, immunological disturbances in the implantation site, and some gynecologic pathologies such as endometriosis, uterine fibroids, hydrosalpinx and endometrial polyps...
May 18, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28135606/hla-g-variability-and-haplotypes-detected-by-massively-parallel-sequencing-procedures-in-the-geographicaly-distinct-population-samples-of-brazil-and-cyprus
#9
Erick C Castelli, Petroula Gerasimou, Michelle A Paz, Jaqueline Ramalho, Iane O P Porto, Thálitta H A Lima, Andréia S Souza, Luciana C Veiga-Castelli, Cristhianna V A Collares, Eduardo A Donadi, Celso T Mendes-Junior, Paul Costeas
The HLA-G molecule presents immunomodulatory properties that might inhibit immune responses when interacting with specific Natural Killer and T cell receptors, such as KIR2DL4, ILT2 and ILT4. Thus, HLA-G might influence the outcome of situations in which fine immune system modulation is required, such as autoimmune diseases, transplants, cancer and pregnancy. The majority of the studies regarding the HLA-G gene variability so far was restricted to a specific gene segment (i.e., promoter, coding or 3' untranslated region), and was performed by using Sanger sequencing and probabilistic models to infer haplotypes...
March 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28129605/mesenchymal-stem-cells-upregulate-treg-cells-via-shla-g-in-sle-patients
#10
Chen Chen, Jun Liang, Genhong Yao, Haifeng Chen, Bingyu Shi, Zhuoya Zhang, Cheng Zhao, Huayong Zhang, Lingyun Sun
BACKGROUND: Soluble human leukocyte antigen-G (sHLA-G) is a non-classical HLA class I molecule, exhibiting strong immunosuppressive properties by inducing the differentiation of T regulatory cells (Treg). Mesenchymal stem cells (MSCs) transplantation alleviates disease progression in systemic lupus erythematosus (SLE) patients. However, the underlying mechanisms are largely unknown. OBJECTIVES: To explore whether sHLA-G is involved in upregulating effects of MSCs on Treg, which contributes to therapeutic effects of MSCs transplantation in SLE...
March 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28093266/comparative-analysis-of-nk-cell-receptor-repertoire-in-adults-and-very-elderly-subjects-with-cytomegalovirus-infection
#11
COMPARATIVE STUDY
Guillermo Juárez-Vega, Velia Rangel-Ramírez, Adriana Monsiváis-Urenda, Perla Niño-Moreno, Christian Garcia-Sepúlveda, Daniel E Noyola, Roberto González-Amaro
Human cytomegalovirus (HCMV) infection in children and young adults has been associated with changes in the innate immune system. We herein analyzed the possible effect of very long term HCMV infection on the expression of several NK cell receptors. Ninety HCMV-seropositive individuals were included and classified as young adults (n=30), elderly (n=30) and very elderly subjects (n=30). A peripheral blood sample was obtained and the expression of NK cell receptors (NKG2A, NKG2C, ILT2, CD161, KIR2DL1, KIR3DL1, and KIR3DL2) by NK and other lymphocyte subsets was assessed by flow cytometry...
March 2017: Human Immunology
https://www.readbyqxmd.com/read/28088953/-research-of-mhla-g-and-the-receptor-expression-with-kidney-rejection-and-cmv-active-infection-following-kidney-transplantation
#12
L L Bi, Y Gao, Y J Sun, X R Kong, X Y He, X H Ma, W H Zhang, L Xiao, B Y Shi
Objective: To study the expression of membrane HLA-G (mHLA-G) and the receptor immunoglobulin-like transcript 2(ILT2) on lymphocyte and find their association with rejection and cytomegalovirus (CMV) infection after renal transplantation. Methods: A total of 88 cases of renal transplant recipients for the first time from February 2014 to February 2016 were studied in this work. Recipients can be divided into rejection group (n=12) and stable renal function group (n=41) according to whether rejection occurred...
January 10, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27320605/hla-g1-but-not-hla-g3-suppresses-human-monocyte-macrophage-mediated-swine-endothelial-cell-lysis
#13
H Eguchi, A Maeda, P C Lo, R Matsuura, E L Esquivel, M Asada, R Sakai, K Nakahata, T Yamamichi, S Umeda, K Deguchi, T Ueno, H Okuyama, S Miyagawa
The inhibitory function of HLA-G1, a class Ib molecule, on monocyte/macrophage-mediated cytotoxicity was examined. The expression of inhibitory receptors that interact with HLA-G, immunoglobulin-like transcript 2 (ILT2), ILT4, and KIR2DL4 (CD158d) on in vitro-generated macrophages obtained from peripheral blood mononuclear cells and the phorbol 12-myristate 13-acetate (PMA)-activated THP-1 cells were examined by flow cytometry. cDNAs of HLA-G1, HLA-G3, HLA-E, and human β2-microglobulin were prepared, transfected into pig endothelial cells (PECs), and macrophage- and the THP-1 cell-mediated PEC cytolysis was then assessed...
May 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27197252/inherent-and-tumor-driven-immune-tolerance-in-the-prostate-microenvironment-impairs-natural-killer-cell-antitumor-activity
#14
Christine Pasero, Gwenaëlle Gravis, Mathilde Guerin, Samuel Granjeaud, Jeanne Thomassin-Piana, Palma Rocchi, Maria Paciencia-Gros, Flora Poizat, Mélanie Bentobji, Francine Azario-Cheillan, Jochen Walz, Naji Salem, Serge Brunelle, Alessandro Moretta, Daniel Olive
The field of immunotherapy for solid tumors, such as prostate cancer, has been recently focusing on therapies that can counter tumor-mediated immunosuppression. Precise quantification and characterization of the immune infiltrates in tumors is crucial to improve treatment efficacy. Natural killer (NK) cells, major components of the antitumor immune system, have never been isolated from prostate tumors, despite their suspected role in disease progression. Here, we examined the frequency, phenotype, and functions of NK cells infiltrating control and tumor prostate tissues...
April 15, 2016: Cancer Research
https://www.readbyqxmd.com/read/27057628/identification-of-novel-micrornas-regulating-hla-g-expression-and-investigating-their-clinical-relevance-in-renal-cell-carcinoma
#15
Simon Jasinski-Bergner, Adi Reches, Christine Stoehr, Chiara Massa, Evamaria Gonschorek, Stefan Huettelmaier, Juliane Braun, Sven Wach, Bernd Wullich, Verena Spath, Ena Wang, Francesco M Marincola, Ofer Mandelboim, Arndt Hartmann, Barbara Seliger
The non-classical human leukocyte antigen G (HLA-G) is expressed at a high frequency in renal cell carcinoma (RCC) and is associated with a higher tumor grade and a poor clinical outcome. This might be caused by the HLA-G-mediated inhibition of the cytotoxicity of T and NK cells. Therefore a selective targeting of HLA-G might represent a powerful strategy to enhance the immunogenicity of RCC lesions. Recent studies identified a number of HLA-G-regulating microRNAs (miRs) and demonstrated an inverse expression of some of these miRs with HLA-G in RCC in vitro and in vivo...
May 3, 2016: Oncotarget
https://www.readbyqxmd.com/read/26874236/multiplex-bead-based-immunoassay-for-the-free-soluble-forms-of-the-hla-g-receptors-ilt2-and-ilt4
#16
Ching-Lien Wu, Signe Goul Svendsen, Adrien Riviere, François Desgrandchamps, Edgardo D Carosella, Joël LeMaoult
Human leukocyte antigen (HLA)-G is an immune-inhibitory molecule that exerts its function via interaction with two main inhibitory receptors: ILT2 and ILT4. This interaction is considered to be an immune checkpoint. HLA-G can be found as a soluble molecule, but it is not known if its receptors can also be found as soluble molecules. In this work, we present a multiplex luminex-based assay to measure soluble ILT2 (sILT2) and soluble ILT4 (sILT4) molecules together. It is based on two antibody pairs, GHI/75 and HP-F1-PE for ILT2 and 27D6 and 42D1-PE for ILT4...
September 2016: Human Immunology
https://www.readbyqxmd.com/read/26711571/altered-dendritic-cell-subset-distribution-in-patients-with-parkinson-s-disease-impact-of-cmv-serostatus
#17
David Goldeck, Walter Maetzler, Daniela Berg, Lilly Oettinger, Graham Pawelec
Parkinson's disease (PD) is characterised by low-level systemic inflammation, which may be at least partly due to pathophysiological activation of immunity. Here, the frequencies of different types of circulating dendritic cells (DCs) with and without a pro-inflammatory phenotype were determined in PD patients and controls. A high proportion of older people is infected with cytomegalovirus (CMV), which acts as a chronic antigenic stressor that could also contribute to increased inflammation. Following this idea, we found higher frequencies of myeloid DCs with a pro-inflammatory CD16+ILT2(high) phenotype in CMV-positive PD patients than controls, suggesting the potential involvement of CMV in exacerbating PD...
January 15, 2016: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/26460949/rescuing-lymphocytes-from-hla-g-immunosuppressive-effects-mediated-by-the-tumor-microenvironment
#18
Danli Wu, Isere Kuiaste, Philippe Moreau, Edgardo Carosella, Patricia Yotnda
Several studies have demonstrated that the antitumor activities of both T and natural killer (NK) effector populations are limited by the immunosuppressive strategies of tumors. In several malignant transformations, the expression of HLA-G by tumor cells rises dramatically, rendering them strongly immunosuppressive. In this study, we postulated that the absence of HLA-G receptors would prevent the immunosuppressive effects of both soluble and membrane-bound HLA-G. Thus, we investigated the therapeutic potential of effector NK cells genetically modified to downregulate the expression of ILT2 (HLA-G receptor) on their cell surfaces...
November 10, 2015: Oncotarget
https://www.readbyqxmd.com/read/26293482/a-phenotypic-analysis-of-regulatory-t-cells-and-uterine-nk-cells-from-first-trimester-pregnancies-and-associations-with-hla-g
#19
Snezana Djurisic, Lillian Skibsted, Thomas Vauvert F Hviid
PROBLEM: The prevalence of regulatory T cells and NK cells expressing activation and HLA-G receptors, and the influence of in vivo sHLA-G and mHLAG on HLA-G receptors expressed by NK cells in the uterine compartment is unclear. METHOD OF STUDY: KIR2DL4 and/or ILT2 expression on regulatory T cells and NK cells from the placental bed and peripheral blood in first trimester was assessed using flow cytometry. Expression of mHLA-G on trophoblast cells and sHLA-G in 'uterine' and peripheral blood was determined with ELISA and flow cytometry, and specific associations with expression levels of cognate receptors or activation markers on immune cells were determined...
November 2015: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/26195547/pseudomonas-aeruginosa-quorum-sensing-molecule-n-3-oxododecanoyl-l-homoserine-lactone-induces-hla-g-expression-in-human-immune-cells
#20
Daria Bortolotti, Joel LeMaoult, Claudio Trapella, Dario Di Luca, Edgardo D Carosella, Roberta Rizzo
HLA-G is a nonclassical class I human leukocyte antigen (HLA) involved in mechanisms of immune tolerance. The objective of this study was to determine whether N-(3-oxododecanoyl)-l-homoserine lactone (3O-C12-HSL), a quorum sensing molecule produced by Pseudomonas aeruginosa, could modify HLA-G expression to control the host immune response. We evaluated the ability of 3O-C12-HSL to induce HLA-G expression in primary immune cells, monocytes (U937 and THP1), and T-cell lines (Jurkat) in vitro and analyzed the cellular pathway responsible for HLA-G expression...
October 2015: Infection and Immunity
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