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https://www.readbyqxmd.com/read/28228149/fasciolopsis-buski-digenea-fasciolidae-from-china-and-india-may-represent-distinct-taxa-based-on-mitochondrial-and-nuclear-ribosomal-dna-sequences
#1
Jun Ma, Miao-Miao Sun, Jun-Jun He, Guo-Hua Liu, Lin Ai, Mu-Xin Chen, Xing-Quan Zhu
BACKGROUND: Fasciolopsis buski is a zoonotic intestinal fluke infecting humans and pigs, but it has been seriously neglected. It is yet to know whether there is any genetic diversity among F. buski from different geographical locations, particularly in sequences of nuclear ribosomal DNA (rDNA) and mitochondrial (mt) DNA. Therefore, we determined the sequences of partial 18S, the complete internal transcribed spacer (ITS) rDNA and the complete mt genome of F. buski from China, compared the rDNA and mtDNA sequences with those of isolates from India and Vietnam, and assessed the phylogenetic relationships of this fluke and related fasciolid trematodes based on the mtDNA dataset...
February 22, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28222637/genotyping-leishmania-promastigotes-isolated-from-patients-with-cutaneous-leishmaniasis-in-south-eastern-turkey
#2
Fahriye Ekşi, Orhan Özgöztaşı, Tekin Karslıgil, Mustafa Sağlam
Objective Cutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods. Methods Novy-MacNeal-Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal transcribed spacer 1 (ITS1) of the Leishmania genome in cultures containing Leishmania promastigotes from 100 patients, which were genotyped with a restriction fragment length polymorphism (RFLP) analysis...
February 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28219759/reevaluating-species-number-distribution-and-endemism-of-the-coral-genus-pocillopora-lamarck-1816-using-species-delimitation-methods-and-microsatellites
#3
P Gélin, B Postaire, C Fauvelot, H Magalon
Species delimitation methods based on genetic information, notably using single locus data, have been proposed as means of increasing the rate of biodiversity description, but can also be used to clarify complex taxonomies. In this study, we explore the species diversity within the cnidarian genus Pocillopora, widely distributed in the tropical belt of the Indo-Pacific Ocean. From 943 Pocillopora colonies sampled in the Western Indian Ocean, the Tropical Southwestern Pacific and Southeast Polynesia, representing a huge variety of morphotypes, we delineated Primary Species Hypotheses (PSH) applying the Automatic Barcode Gap Discovery method, the Poisson tree processes algorithm and the Generalized mixed Yule-coalescent model on two mitochondrial markers (Open Reading Frame and Dloop) and reconstructing a haploweb using one nuclear marker (Internal Transcribed Spacer 2)...
February 17, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28217436/the-first-report-of-onychomycosis-caused-by-cryptococcus-friedmannii-naganishia-friedmannii-a-basidiomycetous-yeast
#4
Masoome Ekhtiari, Shirin Farahyar, Mehraban Falahati, Elham Razmjou, Mahtab Ashrafi-Khozani, Zeinab Ghasemi, Ziba Abbasi-Nejat
Yeasts are common etiologic agents of onychomycosis. This study reported a case of onychomycosis due to Cryptococcus friedmannii (Naganishia friedmannii). This yeast was isolated of the right great toenail of 57-year-old man. Microscopic examination of nail scrapings showed budding cells with thin capsule. Sequence analyzes of the internal transcribed spacer regions was closely related to Cryptococcus friedmannii. The results of susceptibility testing showed the Cryptococcus friedmannii to be sensitive to fluconazole, itraconazole and amphotericin B...
March 2017: Medical Mycology Case Reports
https://www.readbyqxmd.com/read/28216881/specific-pcr-identification-between-peucedanum-praeruptorum-and-angelica-decursiva-and-identification-between-them-and-adulterant-using-dna-barcode
#5
Bang-Xing Han, Yuan Yuan, Lu-Qi Huang, Qun Zhao, Ling-Ling Tan, Xiang-Wen Song, Xiao-Mei He, Tao Xu, Feng Liu, Jian Wang
BACKGROUND: The traditional Chinese medicine (TCM) Qianhu and Zihuaqianhu are the dried roots of Peucedanum praeruptorum and Angelica decursiva, respectively. Since the plant sources of Qianhu and Zihuaqianhu are more complex, the chemical compositions of P. praeruptorum and A. decursiva are significantly different, and many adulterants exist because of the differences in traditional understanding and medication habits. Therefore, the rapid and accurate identification methods are required...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28215215/the-mitochondrial-genome-of-ancylostoma-tubaeforme-from-cats-in-china
#6
X L Shi, Y Q Fu, A Y Abdullahi, M W Wang, F Yang, X G Yu, W D Pan, X X Yan, J X Hang, P Zhang, G Q Li
Ancylostoma tubaeforme may infect canids, felids and humans, and pose a potential risk to public health. Polymerase chain reaction (PCR) techniques were used to amplify the complete mitochondrial (mt) genome sequence of A. tubaeforme from cats and to analyse its sequence characteristics after molecular identification based on the internal transcribed spacer ITS1+ sequence. The results show that the complete mt genome sequence (GenBank accession number KY070315) of A. tubaeforme from cats was 13,730 bp in length, including 12 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, two non-coding regions and an AT-rich region...
February 20, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28205355/trametes-polyzona-an-emerging-filamentous-basidiomycete-in-r%C3%A3-union-island
#7
Alexandre Gauthier, Julien Jaubert, Nicolas Traversier, Jérôme Lemant, Laurent Balu, Dea Garcia-Hermoso, Stephane Welti, Anne Favel, Sandrine Picot, Gautier Hoarau
We describe two serious Trametes polyzona pulmonary infections, which occurred in Réunion Island, in critically ill patients. The identification was performed using sequencing of the internal transcribed spacer region of ribosomal DNA and D1/D2 region of 28S rDNA. In one case, the significance of T. polyzona in the pathological process was certain, proven by histopathological evidence of fungal lung infection. T. polyzona, an emerging filamentous basidiomycete, prevalent in tropical areas, has not been described so far in human infections...
February 16, 2017: Mycoses
https://www.readbyqxmd.com/read/28199443/secondary-structure-of-nrdna-internal-transcribed-spacers-as-a-useful-tool-to-align-highly-divergent-species-in-phylogenetic-studies
#8
Giovanna C Giudicelli, Geraldo Mäder, Gustavo A Silva-Arias, Priscilla M Zamberlan, Sandro L Bonatto, Loreta B Freitas
Recently, it has been suggested that internal transcribed spacer (ITS) sequences are under selective constraints to preserve their secondary structure. Here, we investigate the patterns of the ITS nucleotide and secondary structure conservation across the Passiflora L. genus to evaluate the potential use of secondary structure data as a helpful tool for the alignment in taxonomically complex genera. Considering the frequent use of ITS, this study also presents a perspective on future analyses in other plant groups...
February 13, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28198807/paenibacillus-polymyxa-nsy50-suppresses-fusarium-wilt-in-cucumbers-by-regulating-the-rhizospheric-microbial-community
#9
Lu Shi, Nanshan Du, Sheng Shu, Jin Sun, Shuzhan Li, Shirong Guo
Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. The aim of the present study was to evaluate the effects of NSY50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to Fusarium oxysporum. Bacterial and fungal communities were investigated by Illumina sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) regions (ITS1 and ITS2)...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28195147/geosmin-producing-species-of-coelosphaerium-synechococcales-cyanobacteria-in-lake-shinji-japan
#10
T Godo, Y Saki, Y Nojiri, M Tsujitani, S Sugahara, S Hayashi, H Kamiya, S Ohtani, Y Seike
In Lake Shinji, Japan, periodic outbreaks of musty odour have occurred since mid-May 2007. Although the substance responsible for the odour was identified as geosmin, the odour-producing organism was unknown. We cultivated an axenic unialgal strain and determined that a species of Coelosphaerium (Synechococcales) was responsible for the production of geosmin in Lake Shinji. Our analysis was conducted using gas chromatography/mass spectrometry to determine the odorous compound. To determine the algae species, it was observed by optical microscopy to describe its morphological characteristics and the polymerase chain reaction was used to characterise the nucleotide sequence of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer region...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28195093/molecular-identification-of-candida-species-isolated-from-cases-of-neonatal-candidemia-using-polymerase-chain-reaction-restriction-fragment-length-polymorphism-in-a-tertiary-care-hospital
#11
Akeela Fatima, Gulnaz Bashir, Tehmeena Wani, Abiroo Jan, Amrish Kohli, Mosin S Khan
CONTEXT: Candida spp. is an emerging cause of bloodstream infections worldwide. Delay in speciation of Candida isolates by conventional methods and resistance to antifungal drugs in various Candida species are responsible for the increase in morbidity and mortality due to candidemia. Hence, the rapid identification of Candida isolates is very important for the proper management of patients with candidemia. AIMS: The aim was to re-evaluate the identification of various Candida spp...
January 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28192986/biosynthesis-based-quantitative-analysis-of-151-secondary-metabolites-of-licorice-to-differentiate-medicinal-glycyrrhiza-species-and-their-hybrids
#12
Wei Song, Xue Qiao, Kuan Chen, Ying Wang, Shuai Ji, Jin Feng, Kai Li, Yan Lin, Min Ye
Secondary metabolites are usually the bioactive components of medicinal plants. The difference in the secondary metabolisms of closely related plant species and their hybrids has rarely been addressed. In this study, we conducted a holistic secondary metabolomics analysis of three medicinal Glycyrrhiza species (G. uralensis, G. glabra, and G. inflata), which are used as the popular herbal medicine licorice. The Glycyrrhiza species (genotype) for 95 batches of samples were identified by DNA barcodes of the internal transcribed spacer and trnV-ndhC regions, and the chemotypes were revealed by LC/UV- or LC/MS/MS-based quantitative analysis of 151 bioactive secondary metabolites, including 17 flavonoid glycosides, 24 saponins, and 110 free phenolic compounds...
February 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28187538/first-record-of-the-root-knot-nematode-i-meloidogyne-i-i-minor-i-in-new-zealand-with-description-sequencing-information-and-key-to-known-species-of-i-meloidogyne-i-in-new-zealand
#13
Zeng Qi Zhao, Wellcome Ho, Ruth Griffin, Michael Surrey, Robert Taylor, Lee T Aalders, Nigel L Bell, Yu Mei Xu, Brett J R Alexander
Meloidogyne minor Karssen et al. 2004 was collected from perennial ryegrass (Lolium perenne L.) growing in a sports ground in Christchurch, New Zealand. This is a new record for M. minor, the first report of this nematode occurring in New Zealand, and the second report from the southern hemisphere (after Chile). In general, the New Zealand isolate of M. minor corresponds well to the descriptions of M. minor given by Karssen et al. (2004). The New Zealand isolate is characterized by having a female with dorsally curved stylet, 13-14 μm long, with transversely ovoid knobs slightly sloping backwards from shaft; rounded perineal pattern; and male with stylet 16-19 μm long, large transversely ovoid knobs sloping slightly backwards from shaft; head region not set off, labial disc elevated, lateral lips prominent; and second stage juvenile 370-390 μm long, with hemizonid posterior but adjacent to excretory pore; tail 53-63 μm long; and a distinct hyaline tail terminus 14-18 μm long...
February 9, 2017: Zootaxa
https://www.readbyqxmd.com/read/28182623/evaluation-of-dna-barcodes-in-codonopsis-campanulaceae-and-in-some-large-angiosperm-plant-genera
#14
De-Yi Wang, Qiang Wang, Ying-Li Wang, Xiao-Guo Xiang, Lu-Qi Huang, Xiao-Hua Jin
DNA barcoding is expected to be one of the most promising tools in biological taxonomy. However, there have been no agreements on which core barcode should be used in plants, especially in species-rich genera with wide geographical distributions. To evaluate their discriminatory power in large genera, four of the most widely used DNA barcodes, including three plastid regions (matK, rbcL, trnH-psbA) and nuclear internal transcribed spacer (nrITS), were tested in seven species-rich genera (Ficus, Pedicularis, Rhodiola, Rhododendron,Viburnum, Dendrobium and Lysimachia) and a moderate size genus, Codonopsis...
2017: PloS One
https://www.readbyqxmd.com/read/28181116/diverse-microbial-communities-in-non-aerated-compost-teas-suppress-bacterial-wilt
#15
W K Mengesha, S M Powell, K J Evans, K M Barry
Non-aerated compost teas (NCTs) are water extracts of composted organic materials and are used to suppress soil borne and foliar disease in many pathosystems. Greenhouse trials were used to test the effectiveness of NCTs to suppress potato bacterial wilt caused by Ralstonia solanacearum on plants grown in soils inoculated with a virulent isolate of the pathogen (biovar II). NCTs prepared from matured compost sources: agricultural waste (AWCT), vermicompost (VCT) and solid municipal waste (SMWCT) were evaluated at three initial application times (7 days before inoculation, at time of inoculation and 7 days after inoculation) prior to weekly applications, in a randomized complete-block design...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28177048/use-of-fluorescent-oligonucleotide-probes-for-differentiation-between-paracoccidioides-brasiliensis-and-paracoccidioides-lutzii-in-yeast-and-mycelial-phase
#16
Thales Domingos Arantes, Raquel Cordeiro Theodoro, Marcus de Melo Teixeira, Eduardo Bagagli
BACKGROUND: Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible to culture. OBJECTIVE: In this study, we aimed to standardise an in situ hybridisation technique for the differentiation between the pathogenic species Paracoccidioides brasiliensis and Paracoccidioides lutzii, by using species-specific DNA probes targeting the internal transcribed spacer-1 (ITS-1) of the rRNA gene...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28177044/comparison-of-lamp-and-pcr-for-molecular-mass-screening-of-sand-flies-for-leishmania-martiniquensis-infection
#17
Saruda Tiwananthagorn, Hirotomo Kato, Ranchana Yeewa, Amontip Muengpan, Raxsina Polseela, Saovanee Leelayoova
BACKGROUND: Leishmaniasis caused by Leishmania martiniquensis infection has been reported in human and domestic animals of Martinique Island, Germany, Switzerland, USA, Myanmar and Thailand. The peculiar clinical features of disseminated cutaneous and visceral forms co-existence render the urgent need of specific diagnostic tool to identify the natural sand fly vectors for effective prevention and control strategies. Loop-mediated isothermal amplification (LAMP) of 18S rRNA gene as well as polymerase chain reaction (PCR) of minicircle kinetoplast DNA gene (PCR-mkDNA) have never been applied to detect L...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28176339/phylogeographic-insights-on-the-evolutionary-breakdown-of-heterostyly
#18
Wei Zhou, Spencer C H Barrett, Hai-Dong Li, Zhi-Kun Wu, Xin-Jia Wang, Hong Wang, De-Zhu Li
The breakdown of heterostyly to homostyly is a classic system for the investigation of evolutionary transitions from outcrossing to selfing. Loss of sexual polymorphism is characterized by changes to population morph structure and floral morphology. Here, we used molecular phylogeography to investigate the geographical context for the breakdown process in Primula chungensis, a species with distylous and homostylous populations. We genotyped plants from 20 populations throughout the entire range in south-west China using the chloroplast intergenic spacer (trnL-trnF), nuclear internal transcribed spacer (ITS) and 10 nuclear microsatellite loci, and determined the genetic relationships among populations and the variation in floral traits associated with homostyle evolution...
February 8, 2017: New Phytologist
https://www.readbyqxmd.com/read/28173801/cryptococcus-gattii-molecular-type-vgii-infection-associated-with-lung-disease-in-a-goat
#19
Evelin Catarine da Silva, Juliana Mariotti Guerra, Luciana Neves Torres, Alessandra Maria Dias Lacerda, Raquel Gonçalves Gomes, Danilo Marin Rodrigues, Rodrigo Albergaria Réssio, Priscilla Anne Melville, Camila Cecilia Martin, Fernando José Benesi, Lílian Rose Marques de Sá, Bruno Cogliati
BACKGROUND: Cryptococcus gattii-induced cryptococcosis is an emerging infectious disease of humans and animals with worldwide distribution and public health importance due to its significant morbidity and mortality rate. The present study aimed to report a case of pulmonary infection by C. gattii molecular type VGII in State of São Paulo, Brazil. CASE PRESENTATION: A 5-year-old goat showing intermittent dry cough, ruminal tympany, anorexia, fever, tachycardia and tachypnea was presented for necropsy at the Veterinary Hospital of the School of Veterinary Medicine and Animal Sciences, São Paulo University, São Paulo, Brazil...
February 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28168603/metschnikowia-persici-sp-nov-a-novel-protease-producing-yeast-species-from-china
#20
Chen Wang, Yang Liu, Tao-Tao Zhang, Cai-Ge Lu, Ya Liu, Dian-Peng Zhang, Wei-Cheng Liu
Three yeast strains, named as FHL-A, FHL-B, and FHL-C, were isolated from peach fruit surfaces collected from different regions in the North of China highly produced protease and were presented as single separate group in the genus Metschnikowia by sequence comparisons of 26S rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region. BLASTn alignments on NCBI showed that the similarity of 26S rRNA gene sequences of the three strains to all sequences of other yeasts accessed into the GenBank/EMBL/DDBJ and other database was very low (≦93%)...
February 6, 2017: Current Microbiology
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