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https://www.readbyqxmd.com/read/28920836/mycobacterium-aquiterrae-sp-nov-a-rapidly-growing-bacterium-isolated-from-groundwater
#1
Jae-Chan Lee, Kyung-Sook Whang
A strain representing a rapidly growing, Gram-stain-positive, aerobic, rod-shaped, non-motile, non-sporulating and non-pigmented species of the genus Mycobacterium, designated strain S-I-6T, was isolated from groundwater at Daejeon in Korea. The strain grew at temperatures between 10 and 37 °C (optimal growth at 25 °C), between pH 4.0 and 9.0 (optimal growth at pH 7.0) and at salinities of 0-5 % (w/v) NaCl, growing optimally with 2 % (w/v) NaCl. Phylogenetic analyses based on multilocus sequence analysis of the 16S rRNAgene, hsp65, rpoB and the 16S-23S internal transcribed spacer indicated that strain S-I-6T belonged to the rapidly growing mycobacteria, being most closely related to Mycobacterium sphagni...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920171/an-investigation-of-the-host-specificity-of-metacercariae-of-species-of-apophallus-digenea-heterophyidae-in-freshwater-fishes-using-morphological-experimental-and-molecular-methods
#2
Diána Sándor, Kálmán Molnár, David I Gibson, Csaba Székely, Gábor Majoros, Gábor Cech
Metacercariae of species of the genus Apophallus Lühe, 1909, infecting the fins and skin of freshwater fishes, frequently cause black spot disease. Two species, Apophallus muehlingi (Jägerskiöld, 1899) and A. donicus (Skrjabin & Lindtrop, 1919), are known to occur in Hungarian fishes. It has generally been thought that metacercariae of A. muehlingi infect cyprinid fishes, whereas those of A. donicus develop in percids. As part of a morphological, experimental and molecular study, metacercariae were collected from 99 infected specimens of five cyprinid hosts (Abramis brama, Blicca bjoerkna, Chondrostoma nasus, Squalius cephalus, Scardinius erythrophthalmus) and 18 infected specimens of two percid hosts (Gymnocephalus cernua, Perca fluviatilis) in Hungarian natural waters (Lake Balaton, River Danube)...
September 17, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28918151/internal-transcribed-spacer-its-sequencing-reveals-considerable-fungal-diversity-in-dairy-products
#3
A J Buehler, R L Evanowski, N H Martin, K J Boor, M Wiedmann
Fungi are important spoilage organisms in dairy products. However, little is known about the diversity of naturally occurring spoilage fungi in raw milk and processed dairy products, due at least in part to the fact that classical fungal identification methods require considerable expertise. To gain further insight into the fungal diversity in the dairy system, we isolated fungi from raw milk, raw and pasteurized milk cheese, and yogurt using the selective dichloran rose bengal chloramphenicol agar. In total, 361 fungal isolates were obtained and further characterized by DNA sequencing of the internal transcribed spacer (ITS) region and the nuclear ribosomal large subunit (LSU) rRNA gene if needed...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917305/genetic-variability-of-cloned-cytauxzoon-felis-ribosomal-rna-its1-and-its2-genomic-regions-from-domestic-cats-with-varied-clinical-outcomes-from-five-states
#4
Dana A Pollard, Mason V Reichard, Leah A Cohn, Andrea M James, Patricia J Holman
Cytauxzoon felis is a tick-borne hemoparasite that causes cytauxzoonosis in domestic cats in the United States. Historically, feline cytauxzoonosis was reported to be nearly always fatal. However, increasing evidence of cats surviving acute infection and/or harboring a chronic, subclinical infection has suggested the existence of different C. felis strains that may vary in pathogenicity. In this study, the intraspecific variation of the C. felis first and second ribosomal RNA internal transcribed spacer (ITS1, ITS2) regions was assessed for any clinical outcome or geographic associations...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28912769/adaptation-of-surface-associated-bacteria-to-the-open-ocean-a-genomically-distinct-subpopulation-of-phaeobacter-gallaeciensis-colonizes-pacific-mesozooplankton
#5
Heike M Freese, Anika Methner, Jörg Overmann
The marine Roseobacter group encompasses numerous species which occupy a large variety of ecological niches. However, members of the genus Phaeobacter are specifically adapted to a surface-associated lifestyle and have so far been found nearly exclusively in disjunct, man-made environments including shellfish and fish aquacultures, as well as harbors. Therefore, the possible natural habitats, dispersal and evolution of Phaeobacter spp. have largely remained obscure. Applying a high-throughput cultivation strategy along a longitudinal Pacific transect, the present study revealed for the first time a widespread natural occurrence of Phaeobacter in the marine pelagial...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28912763/the-microbial-community-dynamics-during-the-vitex-honey-ripening-process-in-the-honeycomb
#6
Yaqin Wen, Lin Wang, Yue Jin, Jinzhen Zhang, Lei Su, Xiaoling Zhang, Jinhui Zhou, Yi Li
The bacterial and fungal communities of vitex honey were surveyed by sequencing the 16S rRNA gene and the internal transcribed spacer region of ribosomal DNA. Vitex honey samples were analyzed at different stage of ripening; the vitex flower was also analyzed, and the effect of the chemical composition in the experimental setup was assessed. The results confirmed the presence of dominant Bacillus spp. as the dominant bacterial in honey, and yeast related genera was the main fungal in the honey, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28911667/dna-barcode-and-identification-of-the-varieties-and-provenances-of-taiwan-s-domestic-and-imported-made-teas-using-ribosomal-internal-transcribed-spacer-2-sequences
#7
Shih-Chieh Lee, Chia-Hsiang Wang, Cheng-En Yen, Chieh Chang
The major aim of made tea identification is to identify the variety and provenance of the tea plant. The present experiment used 113 tea plants [Camellia sinensis (L.) O. Kuntze] housed at the Tea Research and Extension Substation, from which 113 internal transcribed spacer 2 (ITS2) fragments, 104 trnL intron, and 98 trnL-trnF intergenic sequence region DNA sequences were successfully sequenced. The similarity of the ITS2 nucleotide sequences between tea plants housed at the Tea Research and Extension Substation was 0...
April 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28910286/potential-dna-barcodes-for-melilotus-species-based-on-five-single-loci-and-their-combinations
#8
Fan Wu, Jinxing Ma, Yuqin Meng, Daiyu Zhang, Blaise Pascal Muvunyi, Kai Luo, Hongyan Di, Wenli Guo, Yanrong Wang, Baochang Feng, Jiyu Zhang
Melilotus, an annual or biennial herb, belongs to the tribe Trifolieae (Leguminosae) and consists of 19 species. As an important green manure crop, diverse Melilotus species have different values as feed and medicine. To identify different Melilotus species, we examined the efficiency of five candidate regions as barcodes, including the internal transcribed spacer (ITS) and two chloroplast loci, rbcL and matK, and two non-coding loci, trnH-psbA and trnL-F. In total, 198 individuals from 98 accessions representing 18 Melilotus species were sequenced for these five potential barcodes...
2017: PloS One
https://www.readbyqxmd.com/read/28906012/pacbio-metabarcoding-of-fungi-and-other-eukaryotes-errors-biases-and-perspectives
#9
Leho Tedersoo, Ave Tooming-Klunderud, Sten Anslan
Second-generation, high-throughput sequencing methods have greatly improved our understanding of the ecology of soil microorganisms, yet the short barcodes (< 500 bp) provide limited taxonomic and phylogenetic information for species discrimination and taxonomic assignment. Here, we utilized the third-generation Pacific Biosciences (PacBio) RSII and Sequel instruments to evaluate the suitability of full-length internal transcribed spacer (ITS) barcodes and longer rRNA gene amplicons for metabarcoding Fungi, Oomycetes and other eukaryotes in soil samples...
September 14, 2017: New Phytologist
https://www.readbyqxmd.com/read/28904417/itaconic-acid-production-by-filamentous-fungi-in-starch-rich-industrial-residues
#10
Richa Bafana, Sarvanadevi Sivanesan, R A Pandey
Several fungi and starch-rich industrial residues were screened for itaconic acid (IA) production. Out of 15 strains, only three fungal strains were found to produce IA, which was confirmed by HPLC and GC-MS analysis. These strains were identified as Aspergillus terreus strains C1 and C2, and Ustilago maydis strain C3 by sequencing of 18S rRNA gene and internal transcribed spacer regions. Cis-aconitate decarboxylase (cad) gene, which encodes a key enzyme in IA production in A. terreus, was characterized from strains C1 and C2...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28899837/an-integrative-taxonomic-study-of-pavanelliella-spp-monogenoidea-dactylogyridae-with-the-description-of-a-new-species-from-the-nasal-cavities-of-an-amazon-pimelodid-catfish
#11
Julio C Aguiar, Antônio A M Maia, Marcia R M Silva, Paulo S Ceccarelli, Marcus V Domingues, Edson A Adriano
The present study is an integrative taxonomic analysis of Pavanelliella spp. (Monogenoidea, Dactylogyridae), and describes a new species from the nasal cavities of the Amazonian pimelodid catfish Brachyplatystoma rousseauxii (Siluriformes Pimelodidae) from the Tapajós River (Amazon Basin, Pará state, Brazil). Pavanelliella jarii sp. n. is characterized by the presence of 3-4 rings in the male copulatory organ, the absence of rings around the vaginal atrium and by its sinuous vaginal canal, which sometimes forms 0...
September 9, 2017: Parasitology International
https://www.readbyqxmd.com/read/28895520/three-novel-species-of-d-xylose-assimilating-yeasts-barnettozyma-xylosiphila-sp-nov-barnettozyma-xylosica-sp-nov-and-wickerhamomyces-xylosivorus-f-a-sp-nov
#12
Ryuichi Kobayashi, Atit Kanti, Hiroko Kawasaki
This study describes three novel xylose-assimilating yeasts, which were isolated from decayed wood collected from Bung Hatta Botanical Garden in West Sumatra and Cibodas Botanic Garden in West Java, or from litter from Eka Karya Bali Botanic Garden in Bali, Indonesia. Phylogenetic analysis was performed based on the sequences of the D1/D2 domains of the large ribosomal subunit (LSU), the small ribosomal subunit (SSU), the internal transcribed spacer (ITS) and elongation factor-1α (EF-1α), and the three strains were found to represent three novel species belonging to genera Barnettozyma or Wickerhamomyces...
September 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28895518/lactobacillus-curtus-sp-nov-isolated-from-beer-in-finland
#13
Yuki Asakawa, Nobuchika Takesue, Shizuka Asano, Satoshi Shimotsu, Kazumaru Iijima, Koji Suzuki, Yasuo Motoyama, Masayuki Aizawa
A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae. However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176T with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S-23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L...
September 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28892290/is-microbial-terroir-related-to-geographic-distance-between-vineyards
#14
Toshiko Miura, Roland Sánchez, Luis E Castañeda, Karina Godoy, Olga Barbosa
While there are substantial studies suggesting that characteristics of wine are related to regional microbial community composition (microbial terroir), there has been little discussion about what factors affect variation in regional microbial community composition. In this study, we compared the microbial community composition of leaves and berries of a grape variety (Carmenere) from six different Chilean vineyards within 35 km of each other. In order to determine relationships between spatial proximity and microbial compositional dissimilarity, we sequenced amplicons of the internal transcribed spacer (ITS) region for fungi and 16S rRNA gene for bacteria...
September 11, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28889913/phylogenetic-relationships-of-chlamydomyces-harzia-olpitrichum-and-their-sexual-allies-melanospora-and-sphaerodes
#15
Neil P Schultes, Besnik Murtishi, De-Wei Li
Phylogenetic analyses using internal transcribed spacer (ITS), large subunit rRNA (LSU), and small subunit (SSU) sequence data showed that Harzia, Chlamydomyces, and Olpitrichum are con-generic. Thus, Chlamydomyces, and Olpitrichum were reduced to synonymy of Harzia. The generic concept was amended and expanded accordingly. Eight new combinations were proposed. Melanospora and Sphaerodes are phylogenetically related to Harzia. However, several members of Melanospora and Sphaerodes are polyphyletic and belong to Hypocreales or Microascales in Sordariomycetes...
October 2017: Fungal Biology
https://www.readbyqxmd.com/read/28889158/detection-and-identification-of-cutaneous-leishmaniasis-isolates-by-culture-polymerase-chain-reaction-and-sequence-analyses-in-syrian-and-central-anatolia-patients
#16
Yunus E Beyhan, Mehmet Karakus, Alper Karagoz, Mesut Mungan, Aysegul T Ozkan, Murat Hokelek
To characterize the cutaneous leishmaniasis (CL) isolates of Syrian and Central Anatolia patients at species levels. Methods: Skin scrapings of 3 patients (2 Syrian, 1 Turkish) were taken and examined by direct examination, culture in Novy-MacNeal-Nicole (NNN) medium, internal transcribed spacer polymerase chain reaction and sequence analysis (PCR). Results:According to microscopic examination, culture and PCR methods, 3 samples were detected positive. The sequencing results of all isolates in the study were identified as Leishmania tropica...
September 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28888828/contribution-of-dark-septate-fungi-to-the-nutrient-uptake-and-growth-of-rice-plants
#17
Carlos Vergara, Karla Emanuelle Campos Araujo, Luiziene Soares Alves, Sônia Regina de Souza, Leandro Azevedo Santos, Claudete Santa-Catarina, Krisle da Silva, Gilmara Maria Duarte Pereira, Gustavo Ribeiro Xavier, Jerri Édson Zilli
The use of dark septate fungi (DSE) to promote plant growth can be beneficial to agriculture, and these organisms are important allies in the search for sustainable agriculture practices. This study investigates the contribution of dark septate fungi to the absorption of nutrients by rice plants and their ensuing growth. Four dark septate fungi isolates that were identified by Internal transcribed spacer phylogeny were inoculated in rice seeds (Cv. Piauí). The resulting root colonization was estimated and the kinetic parameters Vmax and Km were calculated from the nitrate contents of the nutrient solution...
August 23, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28887800/acipensericola-glacialis-n-sp-digenea-aporocotylidae-from-heart-of-lake-sturgeon-acipenser-fulvescens-rafinesque-acipenseriformes-acipenseridae-in-the-great-lakes-basin-lake-winnebago-system-usa
#18
Micah B Warren, Jackson R Roberts, Cova R Arias, Ryan P Koenigs, Stephen A Bullard
Acipensericola glacialis n. sp. infects the heart of lake sturgeon, Acipenser fulvescens (Rafinesque), in the Lake Winnebago System and differs from its only congener, Acipensericola petersoni Bullard, Snyder, Jensen & Overstreet, 2008, by having a dendritic intestine, deeply-lobed testes, a post-ovarian oötype, and a common genital pore that is medial to the dextral caecum. Acipensericola petersoni has a non-dendritic intestine, testes that are not deeply lobed, an oötype that is at level of the ovary (ventral to the ovary), and a common genital pore that is dorsal to the dextral caecum...
September 8, 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28884665/prototheca-blaschkeae-subsp-brasiliensis-subsp-nov-isolated-from-cow-milk
#19
Stefano Morandi, Paola Cremonesi, Milena Povolo, Emanuele Capra, Tiziana Silvetti, Bianca Castiglioni, Márcio Garcia Ribeiro, Ana Carolina Alves, Geraldo Márcio da Costa, Mario Luini, Milena Brasca
A strain of an achlorophyllic alga, named PR24T, was isolated from cow milk samples from the state of Minas Gerais, Brazil. Based on 18S rDNA, 28S rRNA, D1/D2 region of the LSU rDNA and SSU rRNA gene sequence similarities, this strain was found to be a member of the genus Prototheca and closely related to Prototheca blaschkeae SAG2064T. However, the novel strain could easily be distinguished from recognized Prototheca species by internal transcribed spacer, species-specific PCR, single-strand conformation polymorphism-PCR analysis and phenotypic characteristics...
September 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28882174/molecular-phylogeny-of-anopheles-hyrcanus-group-members-based-on-its2-rdna
#20
Yuan Fang, Wen-Qi Shi, Yi Zhang
BACKGROUND: The Anopheles hyrcanus group includes 25 species, and is widely distributed in the Oriental and Palaearctic regions. Several species within this group are vectors of malaria, lymphatic filariasis and Japanese encephalitis. It is difficult or impossible to identify cryptic species based on their morphological characteristics, with some closely related species of the Hyrcanus Group have similar adult morphological characteristics. Thus, their molecular identification has been an important complementary method to traditional morphological taxonomy...
September 7, 2017: Parasites & Vectors
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