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https://www.readbyqxmd.com/read/29783021/a-hyper-diverse-genus-of-acanthocephalans-revealed-by-tree-based-and-non-tree-based-species-delimitation-methods-ten-cryptic-species-of-neoechinorhynchus-in-middle-american-freshwater-fishes
#1
Carlos Daniel Pinacho-Pinacho, Martín García-Varela, Ana L Sereno-Uribe, Gerardo Pérez-Ponce de León
The genus Neoechinorhynchus represents a hyper-diverse group of acanthocephalans, parasites of fresh and brackish water fish and freshwater turtles, with approximately 116 species described worldwide. Forty-nine species have been recorded in the Americas, nine of them in Middle America. Even though species delimitation methods using DNA sequences have been rarely used for parasitic helminths, the genetic library for species of Neoechinorhynchus has grown in the past few years, enhancing the possibility of using these methods for inferring evolutionary relationships and for establishing more robust species boundaries...
May 18, 2018: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/29782003/collection-and-extraction-of-occupational-air-samples-for-analysis-of-fungal-dna
#2
Angela R Lemons, William G Lindsley, Brett J Green
Traditional methods of identifying fungal exposures in occupational environments, such as culture and microscopy-based approaches, have several limitations that have resulted in the exclusion of many species. Advances in the field over the last two decades have led occupational health researchers to turn to molecular-based approaches for identifying fungal hazards. These methods have resulted in the detection of many species within indoor and occupational environments that have not been detected using traditional methods...
May 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781796/starmerella-reginensis-f-a-sp-nov-and-starmerella-kourouensis-f-a-sp-nov-isolated-from-flowers-in-french-guiana
#3
Tiemele Laurent Simon Amoikon, Cécile Grondin, Théodore N'Dédé Djéni, Noémie Jacques, Serge Casaregola
Analysis of yeasts isolated from various biotopes in French Guiana led to the identification of two strains isolated from flowers and designated CLIB 1634T and CLIB 1707T . Comparison of the D1/D2 domain of the large subunit (LSU D1/D2) rRNA gene sequences of CLIB 1634T and CLIB 1707T to those in the GenBank database revealed that these strains belong to the Starmerella clade. Strain CLIB 1634T was shown to diverge from the closely related Starmerella apicola type strain CBS 2868T with a sequence divergence of 1...
May 21, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29781058/hammondia-sp-oocysts-shed-by-a-brazilian-fox-lycalopex-vetulus-differ-from-hammondia-heydorni-and-hammondia-triffittae
#4
Luís F P Gondim, Rodrigo M Soares, Silvia C Osaki, Alessandra Snak, Laura R Grillo, Nelson L M Fernandes, Anderson L de Carvalho
A Brazilian fox (Lycalopex vetulus) was rescued from a highway, and 16 days after maintained in captivity, the fox shed oocysts with sizes compatible with Hammondia sp. and Neospora caninum. DNA extracted from oocysts were initially tested in two PCRs targeting the internal transcribed spacer 1 (ITS-1) of the rDNA of Hammondia heydorni and the Nc-5 gene of N. caninum. A 270-bp product was visualized in the PCR for H. heydorni. No amplification was observed for N. caninum PCR. Since ITS-1-based PCR is not sufficient to differentiate Hammondia species derived from canids, oocyst DNA was examined using multilocus sequence analysis of five genetic fragments [intron 1 of the alpha tubulin gene (intron 1), internal transcribed spaces 1 and 2 (ITS-1 and ITS-2) of the rDNA, 28S rRNA gene (D2/D3 domain), and heat shock protein 70 (Hsp70)]...
May 21, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29780363/microbial-community-analyses-of-the-deteriorated-storeroom-objects-in-the-tianjin-museum-using-culture-independent-and-culture-dependent-approaches
#5
Zijun Liu, Yanhong Zhang, Fengyu Zhang, Cuiting Hu, Genliang Liu, Jiao Pan
In the storeroom C7 of the Tianjin Museum, one wooden desk and two leather luggages dated back to Qing dynasty (1644-1912 AD) presented viable microbial contamination. The aim of the present study was to investigate microbial communities responsible for the biodeterioration of storeroom objects using a combination of culture-independent and culture-dependent methods as well microscopic techniques. Scanning electron microscopy (SEM) revealed that the microflora on three storeroom objects were characterized by a marked presence of Eurotium halophilicum ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780014/evaluation-of-in-vitro-and-in-vivo-drug-efficacy-over-leishmania-tropica-a-pilot-study
#6
Ahmet Özbilgin, İbrahim Çavuş, Ahmet Yıldırım, Tuğba Kaya, Hatice Ertabaklar
OBJECTIVE: Two pentavalent antimonials, meglumine antimoniate (Glucantime®, France) and sodium stibogluconate (Pentostam®, England), are used to treat cutaneous leishmaniasis (CL) in Turkey. The present study, serving as a guidebook for young researchers, aims to provide basis for conducting drug resistance tests and active ingredient scanning in in vitro and in vivo models. METHODS: A CL isolate kept in liquid nitrogen was initially thawed and genotyped by real-time polymerase chain reaction (PCR) using ITS1 prob...
March 2018: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29780013/use-of-internal-transcribed-spacer-sequence-polymorphisms-as-a-method-for-trichomonas-vaginalis-genotyping
#7
Hatice Ertabaklar, Sema Ertuğ, Serçin Özlem Çalışkan, Erdoğan Malatyalı, Bülent Bozdoğan
OBJECTIVE: Trichomonas vaginalis is the most common non-viral, sexually transmitted pathogen with a worldwide distribution. The aim of the present study was to design a new genotyping tool for T. vaginalis isolates using internal transcribed spacer (ITS) sequences. METHODS: First, a total of 20 cryopreserved T. vaginalis isolates were thawed and genomic DNA was isolated from fresh cultures. A polymerase chain reaction (PCR) was performed to amplify the ITS regions and the amplicons were sequenced...
March 2018: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29778767/molecular-morphological-and-experimental-assessment-of-the-life-cycle-of-posthodiplostomum-nanum-trematoda-diplostomidae-from-brazil-with-phylogenetic-evidence-of-the-paraphyly-of-the-genus-posthodiplostomum-dubois-1936
#8
Danimar López-Hernández, Sean A Locke, Alan Lane de Melo, Élida Mara Leite Rabelo, Hudson Alves Pinto
Species of the genus Posthodiplostomum are intestinal parasites of piscivorous birds with worldwide distribution. Recent molecular surveys have focused on developmental stages from vertebrate hosts, with few sequences from larvae found in molluscs. Moreover, most published molecular data are from collections in North America, Europe and Asia, and there are no data for South American species. In the present study, cercariae found in the freshwater limpet Gundlachia ticaga from Brazil were used for morphological, experimental and molecular studies...
May 17, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29778198/molecular-characterization-of-dermatophytes-isolated-from-cattle-in-plateau-state-nigeria
#9
James S Dalis, Haruna M Kazeem, Jacob K P Kwaga, Clara N Kwanashie, Bitrus Yakubu, Olajide A Owolodun, Anvou R Jambol
Dermatophytes from cattle were successfully characterized to species and strain levels for the first time in Nigeria. This study was undertaken to isolate and characterize dermatophytes from cattle in Plateau State, Nigeria. Two molecular techniques were utilized. The first was the use of polymerase chain reaction (PCR) to amplify the internal transcribed spacer regions of the ribosomal DNA using ITS-1 and ITS-4 as primers. This was followed by restriction fragment length polymorphism analysis of the amplified ITS regions using the enzyme MvaI to identify dermatophyte species...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29777991/co-evolutionary-associations-between-root-associated-microbiomes-and-root-transcriptomes-in-wild-and-cultivated-rice-varieties
#10
Lei Tian, Shaohua Shi, Lina Ma, Fahad Nasir, Xiujun Li, Lam-Son Phan Tran, Chunjie Tian
The plants and root-associated microbiomes are closely related. Plant metabolic substances can serve as a nutrient source for the microbiome, and in return, the microbiome can regulate the production of plant metabolic substances. Wild rice (Oryza rufipogon), as the ancestor of cultivated rice (Oryza sativa), has changed several metabolic pathways and root-associated microbiome during evolution. Thus, the study of the different associations between metabolic pathways and root-associated microbiomes in wild and cultivated rice varieties is important for rice breeding...
April 13, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29773434/tinea-blepharo-ciliaris-in-a-13-year-old-girl-caused-by-trichophyton-benhamiae
#11
Fang-Ying Wang, Pei-Lun Sun
Tinea blepharo-ciliaris is a rare form of dermatophyte infection which involves eyelids and associated eyelashes. We report a 13-year-old girl with type I diabetes mellitus who had right eyelid swelling and eyelash loss for two weeks. The lesions were presented as erythematous patches with scales and tiny pustules on the right upper and lower eyelids with broken eyelashes. Two additional annular erythematous patches with scaly active borders were found on her right forearm and right thigh. Microscopic examination of broken eyelashes demonstrated many chains of arthroconidia and hyaline hyphae in an endothrix invasion pattern...
May 14, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29772216/human-liver-fluke-opisthorchis-viverrini-trematoda-opisthorchiidae-in-central-myanmar-new-records-of-adults-and-metacercariae-identified-by-morphology-and-molecular-analysis
#12
Oranuch Sanpool, Win Pa Pa Aung, Rutchanee Rodpai, Wanchai Maleewong, Pewpan M Intapan
Opisthorchis-like metacercariae were found in cyprinoid fish, Puntius brevis, bought from markets in the Bago region, Central Myanmar. Adult worms recovered from experimentally-infected hamsters resembled Opisthorchis viverrini. DNA was extracted from adults and metacercariae. A portion of the mitochondrial cytochrome c oxidase subunit 1 (cox1) and the internal transcribed spacer 2 (ITS2) regions were amplified using the polymerase chain reaction and then sequenced. The sequences confirmed that the flukes were O...
May 14, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29771641/inhabiting-plant-roots-nematodes-and-truffles-polyphilus-a-new-helotialean-genus-with-two-globally-distributed-species
#13
Samad Ashrafi, Dániel G Knapp, Damien Blaudez, Michel Chalot, Jose G Maciá-Vicente, Imre Zagyva, Abdelfattah A Dababat, Wolfgang Maier, Gábor M Kovács
Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode Heterodera filipjevi. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail...
March 26, 2018: Mycologia
https://www.readbyqxmd.com/read/29769615/indoor-wet-cells-as-a-habitat-for-melanized-fungi-opportunistic-pathogens-on-humans-and-other-vertebrates
#14
Xiaofang Wang, Wenying Cai, A H G Gerrits van den Ende, Junmin Zhang, Ting Xie, Liyan Xi, Xiqing Li, Jiufeng Sun, Sybren de Hoog
Indoor wet cells serve as an environmental reservoir for a wide diversity of melanized fungi. A total of 313 melanized fungi were isolated at five locations in Guangzhou, China. Internal transcribed spacer (rDNA ITS) sequencing showed a preponderance of 27 species belonging to 10 genera; 64.22% (n = 201) were known as human opportunists in the orders Chaetothyriales and Venturiales, potentially causing cutaneous and sometimes deep infections. Knufia epidermidis was the most frequently encountered species in bathrooms (n = 26), while in kitchens Ochroconis musae (n = 14), Phialophora oxyspora (n = 12) and P...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29768494/a-new-method-based-on-snp-of-nrdna-its-to-identify-saccharum-spontaneum-and-its-progeny-in-the-genus-saccharum
#15
Shan Yang, Xueting Li, Fei Huang, Yongji Huang, Xinlong Liu, Jiayun Wu, Qinnan Wang, Zuhu Deng, Rukai Chen, Muqing Zhang
The identification of germplasm resources is an important aspect of sugarcane breeding. The aim of this study was to introduce a new method for identifying Saccharum spontaneum and its progeny. First, we cloned and sequenced nuclear ribosomal DNA internal transcribed spacer (nrDNA-ITS) sequences from 20 Saccharum germplasms. Analysis of these nrDNA-ITS sequences showed a stable mutation at base 89. Primers (FO13, RO13, FI16, and RI16) were then designed for tetra-primer amplification refractory mutation system (ARMS) PCR based on mutations at base 89 of the nrDNA-ITS sequence...
2018: PloS One
https://www.readbyqxmd.com/read/29762744/forensically-relevant-blow-flies-in-lebanon-survey-and-identification-using-molecular-markers-diptera-calliphoridae
#16
Salman Shayya, Régis Debruyne, André Nel, Dany Azar
Calliphoridae are among the first insects associated to decomposing animal remains. We have collected 1,841 specimens of three calliphorid genera: Calliphora, Lucilia, and Chrysomya, from different Lebanese localities as a first step in implementing a database of insects of forensic relevance for the country. Blow-flies are crucial for the estimation of the postmortem interval. DNA-based identification is a rapid and accurate method, often used for morphologically similar species, especially for immatures or incomplete specimens...
May 12, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29762080/molecular-identification-of-hysterothylacium-spp-in-fishes-from-the-southern-mediterranean-sea-southern-italy
#17
Antonella Costa, Stefania Graci, Gaetano Cammilleri, Maria Drussilla Buscemi, Rosaria Collura, Antonio Vella, Vincenzo Ferrantelli
In this research a total of 3,017 fish samples belonging to 11 species from Sicily's Mediterranean coasts were examined for the presence of Hysterothylacium spp. larvae, which were then used in subsequent phylogenetic studies. Fifty-eight Raphidascaridid parasitic nematodes were found in the examined fish, with prevalence values ranging from 0.2% in anchovies to 60% in forkbeard samples. Twenty-seven parasites were identified as Hysterothylacium fabri and Hysterothylacium aduncum by sequencing the following regions: the nuclear ribosomal internal transcribed spacer (ITS) and the mitochondrial cytochrome c oxidase subunit II (cox2)...
May 15, 2018: Journal of Parasitology
https://www.readbyqxmd.com/read/29757853/multiplex-polymerase-chain-reaction-assay-for-screening-of-mycotoxin-genes-from-ocular-isolates-of-fusarium-species
#18
Orapin Anutarapongpan, Jorge Maestre-Mesa, Eduardo C Alfonso, Terrence P OʼBrien, Darlene Miller
PURPOSE: To identify mycotoxin genes among clinical ocular isolates of Fusarium species and to correlate these with clinical outcomes of Fusarium keratitis. METHODS: Fifty-four clinical isolates of Fusarium were retrieved from the Bascom Palmer Eye Institute Ocular Microbiology Laboratory data bank. Multiplex polymerase chain reactions were run to confirm the identification of Fusarium species [internal transcribed spacer sequence, translation elongation factor 1-α (TEF) and β-tubulin] and to detect the presence of genes encoding production of fumonisin B mycotoxins (FUM1 and FUM8) and trichothecene mycotoxins (deoxynivalenol and nivalenol)...
May 1, 2018: Cornea
https://www.readbyqxmd.com/read/29745769/comparative-study-of-biofilm-formation-on-biocidal-antifouling-and-fouling-release-coatings-using-next-generation-dna-sequencing
#19
Mark O Winfield, Adrian Downer, Jennifer Longyear, Marie Dale, Gary L A Barker
The bacterial and eukaryotic communities forming biofilms on six different antifouling coatings, three biocidal and three fouling-release, on boards statically submerged in a marine environment were studied using next-generation sequencing. Sequenced amplicons of bacterial 16S ribosomal DNA and eukaryotic ribosomal DNA internal transcribed spacer were assigned taxonomy by comparison to reference databases and relative abundances were calculated. Differences in species composition, bacterial and eukaryotic, and relative abundance were observed between the biofilms on the various coatings; the main difference was between coating type, biocidal compared to fouling-release...
May 10, 2018: Biofouling
https://www.readbyqxmd.com/read/29743617/complete-mitochondrial-and-rdna-complex-sequences-of-important-vector-species-of-biomphalaria-obligatory-hosts-of-the-human-infecting-blood-fluke-schistosoma-mansoni
#20
Si-Ming Zhang, Lijing Bu, Martina R Laidemitt, Lijun Lu, Martin W Mutuku, Gerald M Mkoji, Eric S Loker
Using high throughput Illumina sequencing technology, we determined complete sequences for the mitochondrial genome (mitogenome) and nuclear ribosomal DNA (rDNA) complex for three African freshwater snail taxa within the genus Biomphalaria, B. pfeifferi, B. sudanica and B. choanomphala, and for two laboratory strains of B. glabrata originating from the Neotropics. Biomphalaria snails are obligate vectors of the blood fluke Schistosoma mansoni, a major etiologic agent of human intestinal schistosomiasis. Our data show that mitogenomes from African and Neotropical Biomphalaria are highly conserved...
May 9, 2018: Scientific Reports
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