Alma Hortensia Serafin Muñoz, Kevin Kubachka, Kazimierz Wrobel, J Felix Gutierrez Corona, Santha K V Yathavakilla, Joseph A Caruso, Katarzyna Wrobel
The incorporation of Se to fungi has been studied, focusing on element distribution among different cellular compartments and, in particular, polysaccharide structures contained in cell walls. Se-enriched mycelia of Pleurotus ostreatus were obtained in submerged cultures. The incorporation of selenium from the growth medium to mycelia was observed with the relative distribution between cytosol plus cell membranes fraction (CCM) and cell walls fraction (CW) of about 44 and 56%, respectively. CCM fractions were analyzed by size exclusion chromatography with on-line UV (280 nm) and ICP-MS detection (80Se)...
May 3, 2006: Journal of Agricultural and Food Chemistry