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Counter current chromatography

Hao Yin, Yubai Wei, Rouwen Chen, Si Zhang, Lijuan Long, Hang Yin, Xinpeng Tian, Weihong He
For identifying the synergistic compounds from Pongamia pinnata, an approach based on high-speed counter-current chromatography (HSCCC) combined with isobolographic analysis was designed to detect the synergistic effects in the complex mixture [...].
March 3, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Lihong Zhang, Yanyan Wang, Xiuyun Guo, Shihua Wu
Countercurrent chromatography (CCC) is an efficient separation technique without the solid support matrix, largely depending on the partition of two-immiscible liquid phases in the separation column. Since the helical coil planet centrifuge was invented in early 1970s by Yoichiro Ito, a series of coils columns, including spiral coils and conical coils columns have been developed for CCC separation. In this work, we introduced a new simple and efficient concentrical coils column for CCC separation, which was prepared by winding the whole polytetrafluoroethylene (PTFE) tube into the circular grooves from the rotation axis in the same direction...
March 31, 2017: Journal of Chromatography. A
Yanyan Wang, Lihong Zhang, Hui Zhou, Xiuyun Guo, Shihua Wu
Counter-current chromatography (CCC) is an efficient liquid-liquid partition chromatography technique without support matrix. Despite there are many significant advancements in the CCC separation of natural products especially for non-ionic neutral compounds, CCC isolation of ionic compounds including alkaloids is still a challenging process guide by classical partition coefficients (K) or distribution ratio (KC) because their partition coefficient could not be equal to distribution ratio in common ionic conditions...
March 24, 2017: Journal of Chromatography. A
Sainan Li, Jianxu Zhang, Senlin Li, Chunming Liu, Shu Liu, Zhiqiang Liu
Stroke is one of the most common diseases worldwide. Lactate dehydrogenase inhibitors are widely used in the treatment of ischemic stroke, with natural products considered a promising source of lactate dehydrogenase inhibitors. In this study, ultrafiltration liquid chromatography coupled with mass spectrometry was used for the screening and identification of lactate dehydrogenase inhibitors from Poria cocos. Five lactate dehydrogenase inhibitors were selected: dehydropachymic acid, pachymic acid, dehydrotrametenolic acid, trametenolic acid, and eburicoic acid...
February 20, 2017: Journal of Separation Science
Kwangho Song, Kyoung Jin Lee, Yeong Shik Kim
A novel application of counter-current chromatography (CCC) to enrich plant extracts using direct and continuous injection (CCC-DCI) was developed to fractionate sesquiterpenoids from the buds of Tussilago farfara L. In this study, an n-hexane-acetonitrile-water (HAcW) solvent system was separately pumped into the CCC column, and an extraction solution (45% acetonitrile) was directly and continuously injected into the CCC column. Since the extraction solution was used as a mobile phase in this method, solvent consumption could be greatly reduced...
March 17, 2017: Journal of Chromatography. A
Aneta Spórna-Kucab, Joanna Jagodzińska, Sławomir Wybraniec
Betacyanins, known as antioxidants and chemopreventive natural compounds with colourful properties, were extracted from purple flowers of Gomphrena globosa L. belonging to the Amaranthaceae family and separated for the first time by ion-pair high-speed counter-current chromatography (HSCCC). The pigments were detected by LC-DAD-ESI-MS/MS technique. Separation of betacyanins (300mg) by HSCCC was accomplished in four solvent systems: tert-butyl methyl ether - butanol - acetonitrile - water (0.7% and 1.0% HFBA - heptafluorobutyric acid - system I and III) and tert-butyl methyl ether - butanol - methanol - water (0...
March 17, 2017: Journal of Chromatography. A
Hongmei Li, Tao Chen, Jing Sun, Weiqing Wang, Yulin Li
Two groups of xanthone isomers (1-hydroxy-3,5,8-trimethoxyxanthone and 1-hydroxy-3,7,8-trimethoxyxanthone, 1,8-dihydroxy-3,7-dimethoxyxanthone and 1,8-dihydroxy-3,5-dimethanolxanthone) and two other xanthones (3-methoxy-1,5,8-trihydroxyxanthone and 3,5-dimethoxy-1-hydroxyxanthone) were separated from Swertia franchetiana. First, a solvent system composed of petroleum ether/methanol/water (2:1:0.6, v/v) was developed for the liquid-liquid extraction of these xanthones from the crude extract. Then, an efficient method was established for one step separation of these six xanthones by high-speed counter-current chromatography using n-hexane/ethyl acetate/methanol/ethanol/water (6:4:4:2:4, v/v) as the solvent system...
February 6, 2017: Journal of Separation Science
Daijie Wang, Ning Du, Lei Wen, Heng Zhu, Feng Liu, Xiao Wang, Jinhua Du, Shengbo Li
In this work, the n-butanol extract from leaves of Lonicera japonica Thunb. (L. japonica) was reacted with DPPH and subjected to a HPLC analysis for the guided screening antioxidants (DPPH-HPLC experiments). Then, nine antioxidants, including flavonoid glycosides and caffeoylquinic acid derivatives, were isolated and purified from leaves of L. japonica using high speed counter-current chromatography (HSCCC) and prep-HPLC. The n-butanol extract was firstly isolated by HSCCC using methyl tert-butyl ether/n-butanol/acetonitrile/water (0...
February 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Dabing Ren, Binsong Han, Zhongquan Xin, Shasha Ma, Wenbin Liu, Lunzhao Yi
High-speed countercurrent chromatography combined with preparative high-performance liquid chromatography was successfully used to separate seven phenolic compounds from Stenoloma chusanum Ching. A biphasic solvent system composed of hexane/ethyl acetate/methanol/water (1:2:1:2, v/v) was used for the first step high-speed countercurrent chromatography separation in elution-extrusion mode. A mobile phase composed of acetonitrile (18%) and pure water (82%) was used for further preparative high-performance liquid chromatography purification...
January 25, 2017: Journal of Separation Science
Junfei Tao, Rongwei Yan, Leilei Zhao, Dongmei Wang, Xinjun Xu
10-Deacetylbaccatin III, an important semisynthetic precursor of paclitaxel and docetaxel, can be extracted from Taxus wallichiana Zucc. A process for the isolation and purification of 10-deacetylbaccatin III (1), baccatin III (2), and 7β-xylosyl-10-deacetyltaxol (3) from the leaves and branches of Taxus wallichiana Zucc. via macroporous resin column chromatography combined with high-speed countercurrent chromatography or reversed-phase flash chromatography was developed in this study. After fractionation by macroporous resin column chromatography, 80% methanol fraction was selected based on high-performance liquid chromatography and liquid chromatography with mass spectrometry qualitative analysis...
March 2017: Journal of Separation Science
Martha Knight, Rodrigo Lazo-Portugal, Saeyoung Nate Ahn, Steingrimur Stefansson
Over the last decade man-made carbon nanostructures have shown great promise in electronic applications, but they are produced as very heterogeneous mixtures with different properties so the achievement of a significant commercial application has been elusive. The dimensions of single-wall carbon nanotubes are generally a nanometer wide, up to hundreds of microns long and the carbon nanotubes have anisotropic structures. They are processed to have shorter lengths but they need to be sorted by diameter and chirality...
February 3, 2017: Journal of Chromatography. A
Wenhui Jiang, Hu Shan, Jiying Song, Haitao Lü
A rapid and efficient method for the separation and purification of ombuoside from Gynostemma pentaphyllum by microwave-assisted extraction coupled with high-speed counter-current chromatography (HSCCC) was successfully developed. Using an orthogonal array design L9 (3(4)), the extraction conditions, including microwave power, irradiation time, solid-to-liquid ratio and extraction times, were optimized. Ombuoside was isolated and purified from the crude extraction by HSCCC with two-phase solvent system composed of n-hexane:ethyl acetate:ethanol:water (5:6:5:5, v/v) in a single run...
January 2017: Journal of Chromatographic Science
Eutrophe Le Doux Kamto, Tatiane S C Carvalho, Joséphine Ngo Mbing, Marie C N Matene, Dieudonné E Pegnyemb, Gilda G Leitão
A total of 14 compounds were isolated from the ethanol bark extract of O. kirkii S. Moore (Fabaceae) by alternating isocratic and step gradient elution high-speed counter-current chromatography (HSCCC) methods, using several solvent systems with reference to the polarity of compounds being purified. The extract was successively fractionated with generic solvent systems including n-hexane-ethanol-water (4:2:2) and ethyl acetate-water (1:1). Resulting fractions were further purified using the following preparative gradient elution consisting of ethyl acetate-n-butanol-water (X:Y:10), (X:Y=9:1 (I); 8:2 (II); 7:3 (III); 6:4 (IV); 5:5 (V); 4:6 (VI) 3:7 (VII) and n-hexane- ethyl acetate-methanol-water (1:X:1:1), X=1, 2, 2...
January 13, 2017: Journal of Chromatography. A
Nikhil Kateja, Harshit Agarwal, Vishwanath Hebbi, Anurag S Rathore
Affordability of biopharmaceuticals continues to be a challenge, particularly in developing economies. This has fuelled advancements in manufacturing that can offer higher productivity and better economics without sacrificing product quality in the form of an integrated continuous manufacturing platform. While platform processes for monoclonal antibodies have existed for more than a decade, development of an integrated continuous manufacturing process for bacterial proteins has received relatively scant attention...
December 15, 2016: Biotechnology Progress
Yuwei Zhang, Hua Lin, Shanshan Li, Jianbo Chen, Yinshi Sun, Yuxin Li
High-speed counter-current chromatography was applied for the first time for the separation and purification of bioactive compounds contained in the stem bark of Juglans mandshurica Maxim. Silica gel column chromatography was first used to obtain three composition-enriched target fractions from a crude J. mandshurica methanol extract. Three independent high-speed counter-current chromatography processes were then used to further isolate 13 bioactive compounds, namely, six galloyl glucose derivatives, three flavonones, three naphthoquinones, and ethyl gallate...
December 9, 2016: Journal of Separation Science
Yanyan Wang, Lihong Zhang, Dingding Wang, Xiuyun Guo, Shihua Wu
Counter-current chromatography (CCC) is a solid support-free liquid-liquid partition chromatography and has wide applications. However, CCC separation is still a challenging process and the selection of appropriate solvent system for separation of target compound(s) is still relatively time-consuming. In this work, we introduced a room temperature ionic liquids-based salting-in strategy for the rapid selection of suitable solvent systems for CCC separation. In the randomly selected solvent systems, such as ethyl acetate-water, n-butanol-water, n-pentanol-water, n-hexanol-water, and n-octanol-water, several ionic liquids such as [AMIM]Cl, [MAMIM]Cl, and [BMIM]Cl can increase the solubility of the solutes in the lower phase, which made a dose-dependent decreasing of partition coefficient of solute in the two-phase solvent system...
December 23, 2016: Journal of Chromatography. A
Aihua Peng, Peter Hewitson, Haoyu Ye, Liansuo Zu, Ian Garrard, Ian Sutherland, Lijuan Chen, Svetlana Ignatova
Counter-current chromatography (CCC) has been widely used as a preparative separation method to purify natural products from plant extracts and fermentation broths. Traditionally, throughput optimization in CCC has focused on sample concentration and sample volume. In this paper sample injection was considered as consisting of three variables: injection flow rate, post-injection flow rate and sample solvent. The effects of these parameters were studied using a honokiol purification from a Magnolia officinalis bark extract as a case study aiming to achieve the highest throughput/yield ratio for greater than 99% purity of this potential anti-cancer drug obtained for submission to the Chinese FDA...
December 9, 2016: Journal of Chromatography. A
Kun Cao, Yi Xu, Tian-Ming Zhao, Qing Zhang
BACKGROUND: Sesquiterpenoids, such as tussilagone, has effects of raising blood pressure, antiplatelet aggregation, and anti-inflammation activities, which is regarded as index compound for quality control of Tussilago farfara L. OBJECTIVE: This study was aimed to obtain an effective method for fast isolation of sesquiterpenoids from T. farfara L. by high-speed counter-current chromatography (HSCCC). MATERIALS AND METHODS: A solvent optimization method for HSCCC was presented, i...
October 2016: Pharmacognosy Magazine
Yoichiro Ito, Xiaofeng Ma, Robert Clary
A simple tool is introduced which can modify the shape of tubing to enhance the partition efficiency in high-speed countercurrent chromatography. It consists of a pair of interlocking identical gears, each coaxially holding a pressing wheel to intermittently compress plastic tubing in 0 - 10 mm length at every 1 cm interval. The performance of the processed tubing is examined in protein separation with 1.6 mm ID PTFE tubing intermittently pressed in 3 mm and 10 mm width both at 10 mm intervals at various flow rates and revolution speeds...
2016: Current Chromatography
Artak E Kostanyan
The closed-loop recycling counter-current chromatography (CLR CCC) is performed in several consecutive separation stages. First, the loop is opened, and within a specified time the solution of solutes in the mobile phase is continuously fed to the column. After the solutes loading is finished, the loop is closed, and the first separation stage starts. After a certain number of cycles the first fraction of solutes is eluted, the loop is closed, and the second separation stage starts, and so on. In this study, simple equations are presented allowing the simulation of such separation processes...
November 4, 2016: Journal of Chromatography. A
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