Read by QxMD icon Read

Counter current chromatography

Matic Grom, Mirijam Kozorog, Simon Caserman, Andrej Pohar, Blaž Likozar
Protein A-based affinity chromatography is a highly-efficient separation method to capture, purify and isolate biosimilar monoclonal antibodies (mAb) - an important medical product of biopharmaceutical industrial manufacturing. It is considered the most expensive step in purification downstream operations; therefore, its performance optimization offers a great cost saving in the overall production expenditure. The biochemical mixture-separating specific interaction experiments with Chinese hamster ovary (CHO) cell culture harvest, containing glycosylated extracellular immunoglobulins (Ig), were made using five different state-of-the-art commercial resins...
March 1, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jinzhe He, Peng Fan, Simin Feng, Ping Shao, Peilong Sun
High-speed counter-current chromatography (HSCCC) was used to separate and purify two isoflavones for the first time from Hericium erinaceum ( H. erinaceum ) mycelium using a two-phase solvent system composed of chloroform-dichloromethane-methanol-water (4:2:3:2, v / v / v / v ). These two isoflavones were identified as genistein (4',5,7-trihydroxyisoflavone, C15 H10 O₅) and daidzein (4',7-dihydroxyisoflavone, C15 H10 O₄), using infrared spectroscopy (IR), electro-spary ionisation mass (ESI-MS), ¹H-nuclear magnetic resonance (NMR) and13 C-NMR spectra...
March 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Xuehui Liu, Rongrong Zhou, Bingbing Shen, Lin Chen, Qingyijun Zhou, Dan Wan, Shuihan Zhang
BACKGROUND: Lonicera macranthoides is a Chinese herb that contains a large number of bioactive spanions possessing important pharmacological activities, such as anti-tumour activity. However, detailed information about their anti-tumor activity and bioactive compounds is limited. METHODS: In order to evaluate the scientific basis, the method of high-speed counter-current chromatography (HSCCC) combined with high performance liquid chromatography mass spectrometry (HPLC-ESI-QTOF/MS) has been developed to separate, purify and analyze saponins from Lonicera macranthoides...
February 26, 2018: Current Pharmaceutical Biotechnology
Zhenjia Zheng, Xiao Wang, Pengli Liu, Meng Li, Hongjing Dong, Xuguang Qiao
Burdock roots are healthy dietary supplements and a kind of famous traditional Chinese medicine, which contains large amounts of caffeoylquinic acid derivatives. However, little research has been reported on the preparative separation of these compounds from burdock roots. In the present study, a combinative method of HSCCC and semi-preparative HPLC was developed for the semi-preparative separation of caffeoylquinic acid derivatives from the burdock roots. The ethyl acetate extract of burdock roots was first fractionated by MCI macroporous resin chromatography and give three fractions (Fr...
February 15, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Gabor Jarvas, Marton Szigeti, Andras Guttman
Capillary electrophoresis connected to electrospray ionization mass spectrometry is a promising combination to analyze complex biological samples. The use of sheathless electrospray ionization interfaces, such as a porous nanoelectrospray capillary emitter, requires the application of forward flow (either by pressure or electroosmosis) to maintain the electrospray process. The analysis of solute molecules with strong negative charges (e.g., aminopyrenetrisulfonate labeled glycans) necessitates a reversed-polarity capillary electrophoresis separation mode, in which case the electroosmotic flow is counter current, thus pressure assistance is necessary...
February 19, 2018: Journal of Separation Science
Yi Yang, Jiao Yang, Chen Fang, Dongyu Gu, Ying Ma, Yoichiro Ito
In the present study, a novel model of type-I counter-current chromatography was developed to simplify the separation column manufacture and extend its application ranges. In this system, a multilayer coil was used as the separation column of type-I counter-current chromatography for the first time. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), theoretical plate (N) and peak resolution (Rs) using dipeptides and DNP-amino acids as test samples with two classic solvent systems composed of 1-butanol-acetic acid-water (4:1:5, v/v) (BAW) and hexane-ethyl acetate-methanol-0...
February 13, 2018: Journal of Chromatography. A
Jinru Liang, Wenji Sun
A mass spectrometry guided elution-extrusion counter-current chromatography protocol was developed to separate chemical components from Cornus officinalis Sieb. et Zucc. Ion current extraction, a mass spectrometry based data post-acquisition method, was utilized to boost the separation power and scope of counter-current chromatography technique in this study. As a peak re-picking and denoising tool, ion current extraction was carried out to process the liquid chromatography with mass spectrometry and the counter-current chromatography with mass spectrometry data...
February 14, 2018: Journal of Separation Science
Wenjuan Duan, Wenhua Ji, Yuanan Wei, Ruixuan Zhao, Zijian Chen, Yanling Geng, Feng Jing, Xiao Wang
High-speed counter-current chromatography (HSCCC) coupled with precolumn derivatization was developed for isolating and purifying fructo-oligosaccharides (FOSs). Firstly, the total FOSs were precolumn derivatized and then separated by high-speed counter-current chromatography (HSCCC) with two-phase solvent system petroleum ether- n -butanol-methanol-water (3:2:1:4, v / v ). Secondly, the obtained compounds were deacetylated and the fructo-oligosaccharides (FOSs) with high purity were obtained. Their structures were identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR)...
February 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Makiko Shimizu, Tatsuki Kondo, Tetsuya Fukuoka, Toshihiro Tanaka, Hiroshi Yamazaki
A high activity of cytochrome P450 2D6 (CYP2D6) reportedly leads toxicity of dihydrocodeine/codeine by increasing toxic potential of their metabolite dihydromorphine/morphine, which are further metabolized to highly active dihydromorphine 6-O-glucuronide and the less active morphine 3-O-glucorinide but rapidly excreted into urine as water-soluble forms. A case of acute respiratory depression after administration of prescribed dihydrocodeine phosphate (2.0 mg/day divided TID for 2 days) to a 1-month-old baby boy genotyped as cytochrome CYP2D6*1/*10-*36 is described...
January 30, 2018: Therapeutic Drug Monitoring
Elwira Sieniawska, Rafal Sawicki, Joanna Golus, Marta Swatko-Ossor, Grazyna Ginalska, Krystyna Skalicka-Wozniak
The most commonly used plant source of β-elemene is Curcuma wenyujin Y. H. Chen & C. Ling (syn. of Curcuma aromatic Salisb.) with its content in supercritical CO₂ extract up to 27.83%. However, the other rich source of this compound is Nigella damascena L. essential oil, in which β-elemene accounts for 47%. In this work, the effective protocol for preparative isolation of β-elemene from a new source-N. damascena essential oil-using high performance counter-current chromatography HPCCC was elaborated...
January 28, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jonatas Vicente Milato, Raphael Salles Ferreira Silva, Fabiana de Souza Figueiredo, Debora de Almeida Azevedo, Carlos Alexandre Bulchi Ribeiro, Gilda Guimarães Leitão
A solvent system was developed for selective isolation by high-speed counter-current chromatography (HSCCC) of the benzoquinone 7α-hydroxyroileanone, 1, a bioactive diterpene from a dichloromethane extract of Tetradenia riparia leaves. Several solvent systems were initially studied, including hexane-ethyl acetate-methanol-water in several ratios, hexane-acetone-methanol-water, hexane-ethanol-water and hexane-acetonitrile-methanol, which gave recovery rates for the target compound between 13.4 and 35.9%. The new solvent system hexane-5% aqueous Na2CO3 (1:1) was developed based on the chemical ionization reaction of the benzoquinone hydroxyl group in the basic pH of the carbonate solution, prompted by the extraction procedure used for the extraction of lapachol (a natural naphtoquinone) from a Tabebuia species wood...
January 9, 2018: Journal of Chromatography. A
Lin Zhang, Sandeep Yadav, Y John Wang, Olivier Mozziconacci, Christian Schӧneich
PURPOSE: L-Histidine (L-His) and polysorbate 20 (PS20) are two excipients frequently included in parenteral products to stabilize biotherapeutics. The objective of the current work was to investigate the impact of L-His on PS20 stability in aqueous solutions when subjected to forced oxidation and accelerated stability testing. METHODS: The stability of PS20 in L-His buffer was compared with that in acetate buffer. Forced oxidation of PS20 in these two buffer systems was initiated by a free radical generator, 2,2'-azobis (2-amidinopropane) hydrochloride (AAPH), while accelerated stability tests were carried out at 40°C...
January 16, 2018: Pharmaceutical Research
Tao Chen, Ping Wang, Nana Wang, Chongyang Sun, Xue Yang, Hongmei Li, Guoying Zhou, Yulin Li
The separation of polar compounds by high-speed counter-current chromatography is still regarded as a challenge. In this study, an efficient strategy for the separation of three polar compounds from Rheum tanguticum has been successfully conducted by using high-speed counter-current chromatography. X-5 macroporous resin chromatography was used for the fast enrichment of the target compounds. Then, the target fraction was directly introduced into high-speed counter-current chromatography for separation using ethyl acetate/glacial acetic acid/water (100:1:100, v/v) as the solvent system...
January 13, 2018: Journal of Separation Science
Jia Xiong, Mary H Grace, Debora Esposito, Slavko Komarnytsky, Fei Wang, Mary Ann Lila
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g-1, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g-1, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264...
November 2017: Chinese Journal of Natural Medicines
Yanyan Wang, Lihong Zhang, Xiuyun Guo, Shihua Wu
Ionic liquids have been widely used for the extraction and separation of bioactive natural and synthetic mixtures. In this study, we provided an updated example by using an ionic liquid-based salting-in counter-current chromatography (CCC) strategy for the separation of hydrophobic tanshinones without subsequent column chromatography purification. Several ionic liquids such as 1-allyl-3-methylimidazolium chloride ([AMIM]Cl), 1-methallyl-3-methylimidazolium chloride ([MAMIM]Cl) and 1-butyl-3-ethylimidazolium chloride [BMIM]Cl could significantly decrease the partition coefficients (K) of tanshinones in the selected two-phase solvent composed of hexane-ethyl acetate-methanol-ionic liquid aqueous solution (5:5:6:4, v/v)...
December 26, 2017: Journal of Chromatography. A
Denglang Zou, Xuelin Zhu, Fan Zhang, Yurong Du, Jianbin Ma, Renwang Jiang
This study presents an efficient strategy based on liquid-liquid extraction with three-phase solvent system and high speed counter-current chromatography for rapid enrichment and separation of epimers of minor bufadienolide from toad meat. The reflux extraction conditions were optimized by response surface methodology firstly and a novel three-phase solvent system composed of n-hexane/methyl acetate/acetonitrile/water (3:6:5:5, v/v) was developed for liquid-liquid extraction of the crude extract. This integrative extraction process could enrich minor bufadienolide from complex matrix efficiently and minimize the loss of minor targets induced by repeated extraction with different kinds of organic solvent occurred in the classical liquid two-phase extraction...
January 4, 2018: Journal of Agricultural and Food Chemistry
Dan Wang, Yan Mu, Hongjing Dong, Huijiao Yan, Cui Hao, Xiao Wang, Lisi Zhang
Diaphragma juglandis fructus contains various bioactive constituents. Fourteen compounds were isolated from Diaphragma juglandis fructus by preparative high performance liquid chromatography (pre-HPLC) and high-speed counter-current chromatography (HSCCC). Their structures were identified by nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). Compounds (+)-dehydrovomifoliol (12), (6R,9R)-9-hydroxymegastigman-4-en-3-one (13) and (6R,9S)-9-hydroxymegastigman-4-en-3-one (14) are found from Juglans regia L...
December 29, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Hui Zou, Yan Ma, Zhenzhen Xu, Xiaojun Liao, Ailiang Chen, Shuming Yang
Three anthocyanins were isolated from strawberry extract by high-speed counter-current chromatography, using a biphasic mixture of tert-butyl methyl ether, n-butanol, acetonitrile, water and trifluoroacetic acid (2.5:2.0:2.5:5.0:1.0%). The anthocyanins were identified as pelargonidin-3-rutinoside, cyanidin-3-glucoside and pelargonidin-3-glucoside with purity of 95.6%, 96.2% and 99.3% respectively. Additionally, the copigmentation reaction rates between pelargonidin-3-glucoside and phenolic acids (catechin or epicatechin) at pH 1...
May 1, 2018: Food Chemistry
Aneta Spórna-Kucab, Angelika Milo, Agnieszka Kumorkiewicz, Sławomir Wybraniec
Betacyanins, natural plant pigments exhibiting antioxidant and chemopreventive properties, were extracted from Celosia spicata (Thouars) Spreng. inflorescences and separated by high-speed counter-current chromatography (HSCCC) in two polar solvent systems composed of: TBME - 1-BuOH - ACN - H2O (0.7% HFBA, 2:2:1:5, v/v/v/v) (system I) and EtOH - ACN - 1-PrOH - (NH4)2SO4satd.soln - H2O (0.5:0.5:0.5:1.2:1, v/v/v/v/v) (system II). The systems were used in the head-to-tail (system I) and tail-to-head (system II) mode...
November 23, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Chao Han, Wenli Wang, Guimin Xue, Dingqiao Xu, Tianyu Zhu, Shanshan Wang, Pei Cai, Jianguang Luo, Lingyi Kong
Cu(II) ion was selected as an additive to improve the enantioseparation efficiency of three dihydroflavone enantiomers in high-speed counter-current chromatography (HSCCC), using hydroxypropyl-β-cyclodextrin (HP-β-CyD) as the chiral selector. The influences of important parameters, including the metal ion, the concentrations of HP-β-CyD and the Cu(II) ion, and the sample size were investigated. Under optimal conditions, three dihydroflavone enantiomers, including (±)-hesperetin, (±)-naringenin, and (±)-farrerol, were successfully enantioseparated...
January 12, 2018: Journal of Chromatography. A
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"