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https://www.readbyqxmd.com/read/28545258/marker-assisted-molecular-profiling-deletion-mutant-analysis-and-rna-seq-reveal-a-disease-resistance-cluster-associated-with-uromyces-appendiculatus-infection-in-common-bean-phaseolus-vulgaris-l
#1
Antonette R Todd, Nicole Donofrio, Venkateswara R Sripathi, Phillip E McClean, Rian K Lee, Marcial Pastor-Corrales, Venu Kal Kalavacharla
Common bean (Phaseolus vulgaris L.) is an important legume, useful for its high protein and dietary fiber. The fungal pathogen Uromyces appendiculatus (Pers.) Unger can cause major loss in susceptible varieties of the common bean. The Ur-3 locus provides race specific resistance to virulent strains or races of the bean rust pathogen along with Crg, (Complements resistance gene), which is required for Ur-3-mediated rust resistance. In this study, we inoculated two common bean genotypes (resistant "Sierra" and susceptible crg) with rust race 53 of U...
May 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28545231/atmospheric-sampling-on-ascension-island-using-multirotor-uavs
#2
Colin Greatwood, Thomas S Richardson, Jim Freer, Rick M Thomas, A Rob MacKenzie, Rebecca Brownlow, David Lowry, Rebecca E Fisher, Euan G Nisbet
As part of an NERC-funded project investigating the southern methane anomaly, a team drawn from the Universities of Bristol, Birmingham and Royal Holloway flew small unmanned multirotors from Ascension Island for the purposes of atmospheric sampling. The objective of these flights was to collect air samples from below, within and above a persistent atmospheric feature, the Trade Wind Inversion, in order to characterise methane concentrations and their isotopic composition. These parameters allow the methane in the different air masses to be tied to different source locations, which can be further analysed using back trajectory atmospheric computer modelling...
May 23, 2017: Sensors
https://www.readbyqxmd.com/read/28544820/delays-to-care-in-pediatric-lupus-patients-from-the-childhood-arthritis-and-rheumatology-research-alliance-legacy-registry
#3
Tamar B Rubinstein, Wenzhu B Mowrey, Norman T Ilowite, Dawn M Wahezi
OBJECTIVES: Prompt treatment for lupus is important to prevent morbidity. A potential barrier to early treatment of pediatric lupus is delayed presentation to a pediatric rheumatologist. To better understand factors contributing to delayed presentation among pediatric lupus patients, we examined differences in demographic and clinical characteristics of lupus patients within the Childhood Arthritis and Rheumatology Research Alliance (CARRA) Legacy Registry with regards to time between symptom onset and presentation to a pediatric rheumatologist...
May 23, 2017: Arthritis Care & Research
https://www.readbyqxmd.com/read/28544327/genetic-variation-at-the-th2-immune-gene-il13-is-associated-with-ige-mediated-paediatric-food-allergy
#4
S E Ashley, H-T T Tan, R Peters, K J Allen, P Vuillermin, S C Dharmage, M L K Tang, J Koplin, A Lowe, A-L Ponsonby, J Molloy, M C Matheson, R Saffery, J A Ellis, D Martino
BACKGROUND: Food allergies pose a considerable world-wide public health burden with incidence as high as one in ten in 12-month-old infants. Few food allergy genetic risk variants have yet been identified. The Th2 immune gene IL13 is a highly plausible genetic candidate as it is central to the initiation of IgE class switching in B cells. OBJECTIVE: Here, we sought to investigate whether genetic polymorphisms at IL13 are associated with the development of challenge-proven IgE-mediated food allergy...
April 24, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28542065/data-for-community-health-assessment-in-rural-colorado-a-comparison-of-electronic-health-records-to-public-health-surveys-to-describe-childhood-obesity
#5
Margaret J Gutilla, Arthur J Davidson, Matthew F Daley, G Brooke Anderson, Julie A Marshall, Sheryl Magzamen
CONTEXT: Community-level data are necessary to inform community health assessments and to plan for appropriate interventions. However, data derived from public health surveys may be limited or unavailable in rural locations. OBJECTIVE: We compared 2 sources of data for community health assessment in rural Colorado, electronic health records (EHRs) and routine public health surveys. DESIGN: Comparison of cross-sectional measures of childhood/youth obesity prevalence and data quality...
July 2017: Journal of Public Health Management and Practice: JPHMP
https://www.readbyqxmd.com/read/28541483/malaria-host-candidate-genes-validated-by-association-with-current-recent-and-historic-measures-of-transmission-intensity
#6
Nuno Sepúlveda, Alphaxard Manjurano, Susana G Campino, Martha Lemnge, John Lusingu, Raimos Olomi, Kirk A Rockett, Christina Hubbart, Anna Jeffreys, Kate Rowlands, Taane G Clark, Eleanor M Riley, Chris J Drakeley
Background: Human malaria susceptibility is determined by multiple genetic factors. It is however unclear which genetic variants remain important over time. Methods: Genetic associations of 175 high quality polymorphisms within several malaria candidate genes were examined in a sample of 8,096 individuals from northeast Tanzania using altitude, seroconversion rates and parasite rates as proxies of historical, recent and current malaria transmission intensity. A principal component (PC) analysis was used to derive two alternative measures of overall malaria propensity of a location across different time scales...
May 25, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28540423/direct-treatment-of-high-strength-soft-drink-wastewater-using-a-down-flow-hanging-sponge-reactor-performance-and-microbial-community-dynamics
#7
Junhui Liao, Curtis Fang, Jimmy Yu, Arun Sathyagal, Eric Willman, Wen-Tso Liu
A stand-alone down-flow hanging sponge (DHS) system with a two-stage configuration was operated for 700 days to treat synthetic soft drink wastewater at 3000 mg/L chemical oxygen demand (COD). Throughout the operation, >90% COD and total organic carbon (TOC) removal efficiency was obtained by the first stage, and a final effluent of COD <60 mg/L (TOC <20 mg/L) was consistently maintained with the second stage. Lower organic removal efficiency was observed to closely correlate with lower pH, higher volatile fatty acid (VFA) concentration, and higher suspended solid (SS) in the effluent...
May 24, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28537240/-point-mutations-in-the-dna-binding-domain-of-p53-contribute-to-glioma-progression-and-poor-prognosis
#8
P P Sarma, D Dutta, Z Mirza, K Kr Saikia, B Kr Baishya
TP53 mutations play a significant role in glioma tumorigenesis. When located in in the DNA binding domain, these mutations can perturb p53 protein conformation and its function, often culminating in altered downstream signaling. Here we describe prevalent pattern of TP53 point mutations in a cohort of 40 glioma patients and show their relevance to gliomagenesis. Point mutations in exon 5-9 of TP53 gene were detected by DNA sequencing. Possible influence of identified mutations at the function of p53 was studied computationally and correlated with the survival...
March 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28537034/polycyclic-aromatic-hydrocarbons-in-urban-soil-of-novi-sad-serbia-occurrence-and-cancer-risk-assessment
#9
Biljana D Škrbić, Nataša Đurišić-Mladenović, Đorđe J Tadić, Jelena Đ Cvejanov
Contents of 16 polycyclic aromatic hydrocarbons were analyzed in 30 soil samples from 15 locations in Novi Sad, Serbia, assessing for the first time the corresponding health risks in the Serbian urban zone. Total concentrations were in the range of 22-2247 μg kg(-1), with a mean and median value of 363 and 200 μg kg(-1), respectively. Comparison with the relevant maximum allowed contents proposed by the Serbian government and with the Dutch target values implied that soils from the urban area of Novi Sad were "suitable as residential soils" and that no intervention would be needed if the current levels were retained...
May 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28536289/modulation-of-aneuploidy-in-leishmania-donovani-during-adaptation-to-different-in-vitro-and-in-vivo-environments-and-its-impact-on-gene-expression
#10
F Dumetz, H Imamura, M Sanders, V Seblova, J Myskova, P Pescher, M Vanaerschot, C J Meehan, B Cuypers, G De Muylder, G F Späth, G Bussotti, J R Vermeesch, M Berriman, J A Cotton, P Volf, J C Dujardin, M A Domagalska
Aneuploidy is usually deleterious in multicellular organisms but appears to be tolerated and potentially beneficial in unicellular organisms, including pathogens. Leishmania, a major protozoan parasite, is emerging as a new model for aneuploidy, since in vitro-cultivated strains are highly aneuploid, with interstrain diversity and intrastrain mosaicism. The alternation of two life stages in different environments (extracellular promastigotes and intracellular amastigotes) offers a unique opportunity to study the impact of environment on aneuploidy and gene expression...
May 23, 2017: MBio
https://www.readbyqxmd.com/read/28536071/analysis-of-anelloviridae-sequences-characterized-from-serial-human-and-animal-biological-samples
#11
Sandra Bédarida, Bertrand Dussol, Michel Signoli, Philippe Biagini
Rolling-circle amplification-sequence-independent single primer amplifications (RCA-SISPA) and/or RCA-PCR-based approaches were applied to serial human plasma and animal (domestic cat) saliva samples. Complete SENV-H-related and PRA4 Anelloviridae genomes were characterized and analysed over time (~16 and 6.5years for human and animal samples, respectively). Genomic sequences and deduced putative coding regions were compared. Comparable values, i.e. ~2×10(-4)subs/site/year, were obtained for estimated rates of non-synonymous substitutions...
May 20, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28535591/effect-of-water-stage-and-tree-stand-composition-on-spatiotemporal-differentiation-of-spring-water-chemistry-draining-carpathian-flysch-slopes-gorce-mts
#12
Michał Jasik, Stanisław Małek, Mirosław Żelazny
The purpose of this study was to identify the factors affecting spring water chemistry in different tree stands and to measure the influence of water stage on the physicochemical parameters of spring waters in a small Carpathian catchment. Water samples were collected three times per year at various stages of the water: after the spring thaw, after a period of heavy rain and after a dry period in 2011 and 2012. Water samples were left in the laboratory to reach room temperature (19-20°C) and analyzed for EC (reference T=25°C) and pH...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535589/water-soluble-organic-aerosols-in-indo-gangetic-plain-igp-insights-from-aerosol-mass-spectrometry
#13
Abhishek Chakraborty, Pradhi Rajeev, Prashant Rajput, Tarun Gupta
Filter samples collected during winter of 2015-16 from two polluted urban locations (Allahabad and Kanpur) residing within Indo-Gangetic plain (IGP) showed high levels of water-soluble organic aerosols (WSOA). Total organic aerosols (OA) in submicron fraction, measured at Kanpur in real time via Aerosol Mass Spectrometer also showed substantially high concentration levels. WSOA to OA contribution in Kanpur was found to be very high (around 55%) indicating significant contributions from secondary OA (SOA). On average, WSOA oxidation ratio (O/C) was found to be higher (15-20%) in Kanpur than at Allahabad...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535562/isocentric-navigation-of-percutaneous-endoscopic-transforaminal-discectomy-at-the-l5-s1-level-in-difficult-puncture-cases-a-technical-note
#14
Guoxin Fan, Teng Wang, Shuo Hu, Xiaofei Guan, Xin Gu, Shisheng He
BACKGROUND: Accurate puncture during percutaneous transforaminal endoscopic discectomy at the L5/S1 level in cases with high iliac crest and narrow foramen were difficult, even though the difficulties of foraminoplasty could be overcome by advanced instruments like reamers. OBJECTIVES: The report aimed to describe an isocentric navigation technique with a definite pathway in difficult puncture cases at the L5/S1 level. STUDY DESIGN: Technical note...
May 2017: Pain Physician
https://www.readbyqxmd.com/read/28535345/natural-predominance-of-abscisic-acid-in-pongammia-pinnata-karanj-honey-contributed-to-its-strong-antimutagenicity
#15
Sudhanshu Saxena, Jyoti Tripathi, Suchandra Chatterjee, Satyendra Gautam
Various samples of raw (unprocessed) floral honey collected from different geographical locations of India were assayed for its antimutagenicity against ethyl methanesulfonate in E. coli MG1655 cells through rifampicin resistance assay. A monofloral honey ('Pongammia pinnata', local name 'Karanj') displayed maximum antimutagenicity (78.0 ± 1.7; P≤0.05). Solid phase extraction (using amberlite XAD-2 resin) followed by HPLC resulted into different peaks displaying varying antimutagenicity. Peak at retention time (Rt) 27...
May 23, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28535339/ecophysiological-examination-of-the-lake-erie-microcystis-bloom-in-2014-linkages-between-biology-and-the-water-supply-shutdown-of-toledo-ohio
#16
Morgan Michelle Steffen, Timothy W Davis, Robert Michael McKay, George S Bullerjahn, Lauren E Krausfeldt, Joshua M A Stough, Michelle L Neitzey, Naomi E Gilbert, Gregory L Boyer, Thomas H Johengen, Duane C Gossiaux, Ashley M Burtner, Danna Palladino, Mark Rowe, Gregory J Dick, Kevin Meyer, Shawn Levy, Braden Boone, Richard Stumpf, Timothy Wynne, Paul V Zimba, Danielle B Gutierrez, Steven W Wilhelm
Annual cyanobacterial blooms dominated by Microcystis have occurred in western Lake Erie (USA/Canada) during summer months since 1995. The production of toxins by bloom-forming cyanobacteria can lead to drinking water crises, such as the one experienced by the city of Toledo in August of 2014, when the city was rendered without drinking water for > 2 days. It is important to understand the conditions and environmental cues that were driving this specific bloom to provide a scientific framework for management of future bloom events...
May 23, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28532940/effect-of-individual-level-and-socioeconomic-factors-on-long-term-survival-after-cataract-surgery-over-a%C3%A2-30-year%C3%A2-period
#17
Claudia Geue, Sven Jonuscheit
PURPOSE: To evaluate survival and the risk for mortality after cataract surgery in relation to individual-level and socioeconomic factors in Scotland over 3 decades. SETTING: Linked healthcare data, United Kingdom. DESIGN: Representative population-based study. METHODS: A 5% random sample of Scottish decedents linked to hospital records (1981 to 2012) was assessed. Survival time, survival probability, and determinants of mortality were evaluated after the first and second recorded hospital episodes for cataract surgery...
April 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28530346/-seeds-of-mindfulness-in-the-desert-preliminary-results-from-a-clinical-trial-in-the-mindfulness-clinic-mental-health-center-beer-sheva
#18
Lee Tibi Cohen, Keren Reiner Noy, Ayelet Shmulevich, Oded Arbel
INTRODUCTION: Mindfulness-based therapies are rooted in the Buddhist tradition, and promote cultivation of qualities such as open awareness, acceptance and compassion. While various studies have demonstrated the efficacy of integrating mindfulness in therapeutic practice for people suffering from clinical conditions such as depression and anxiety as well as the non-clinical population, this was rarely replicated in Israel. In the Mindfulness Clinic, located in the Mental Health Center, Beer Sheva, mindfulness workshops are now available for diverse populations dealing with various challenges such as daily stress, chronic pain, and depression...
December 2016: Harefuah
https://www.readbyqxmd.com/read/28528416/time-trends-of-persistent-organic-pollutants-in-benthic-and-pelagic-indicator-fishes-from-puget-sound-washington-usa
#19
James E West, Sandra M O'Neill, Gina M Ylitalo
We modeled temporal trends in polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and dichlorodiphenyltrichloroethane and its metabolites (DDTs) in two indicator fish species representing benthic and pelagic habitats in Puget Sound, Washington, USA. English sole (Parophrys vetulus, benthic) index sites and larger-scale Pacific herring (Clupea pallasii, pelagic) foraging areas represented a wide range of possible contamination conditions, with sampling locations situated adjacent to watersheds exhibiting high, medium and low development...
May 20, 2017: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28528277/determining-the-number-of-test-fires-needed-to-represent-the-variability-present-within-9mm-luger-firearms
#20
Eric F Law, Keith B Morris, Casey M Jelsema
Many studies have been performed in recent years in the field of firearm examination with the goal of providing an objective method for comparisons of fired cartridge cases. No published research to support the number of test fires needed to represent the variability present within the impressions left on a cartridge case could be found. When a suspect firearm is submitted to a firearm examiner, typically two to four test fires are performed. The recovered cartridge cases are compared to each other to determine which characteristics from the firearm are reproducing, and then compared to any cartridge cases collected at a crime scene...
May 3, 2017: Forensic Science International
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