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https://www.readbyqxmd.com/read/29352392/impact-of-dexamethasone-concentration-on-cartilage-tissue-formation-from-human-synovial-derived-stem-cells-in-vitro
#1
Ryota Chijimatsu, Masato Kobayashi, Kosuke Ebina, Toru Iwahashi, Yosuke Okuno, Makoto Hirao, Atsunori Fukuhara, Norimasa Nakamura, Hideki Yoshikawa
Human synovial mesenchymal stem cells (hSMSCs) are a promising cell source for cartilage regeneration because of their superior chondrogenic potential in vitro. This study aimed to further optimize the conditions for inducing chondrogenesis of hSMSCs, focusing on the dose of dexamethasone in combination with transforming growth factor-β3 (TGFβ3) and/or bone morphogenetic protein-2 (BMP2). When hSMSCs-derived aggregates were cultured with TGFβ3, dexamethasone up to 10 nM promoted chondrogenesis, but attenuated it with heterogeneous tissue formation when used at concentrations over than 100 nM...
January 19, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29352270/non-canonical-wnt-induces-chondrocyte-de-differentiation-through-frizzled-6-and-dvl-2-b-raf-camkii%C3%AE-syndecan-4-axis
#2
Zhe Xie, Mostafa Khair, Irfan Shaukat, Patrick Netter, Didier Mainard, Lydia Barré, Mohamed Ouzzine
Dysregulation of Wnt signaling has been implicated in developmental defects and in the pathogenesis of many diseases such as osteoarthritis; however, the underlying mechanisms are poorly understood. Here, we report that non-canonical Wnt signaling induced loss of chondrocyte phenotype through activation of Fz-6/DVL-2/SYND4/CaMKIIα/B-raf/ERK1/2 cascade. We show that in response to Wnt-3a, Frizzled 6 (Fz-6) triggers the docking of CaMKIIα to syndecan 4 (SYND4) and that of B-raf to DVL-2, leading to the phosphorylation of B-raf by CaMKIIα and activation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) signaling, which leads to chondrocyte de-differentiation...
January 19, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29350647/rotator-cuff-calcific-tendinopathy-from-diagnosis-to-treatment
#3
Vito Chianca, Domenico Albano, Carmelo Messina, Federico Midiri, Giovanni Mauri, Alberto Aliprandi, Michele Catapano, Lorenzo Carlo Pescatori, Cristian Giuseppe Monaco, Salvatore Gitto, Anna Pisani Mainini, Angelo Corazza, Santi Rapisarda, Grazia Pozzi, Antonio Barile, Carlo Masciocchi, Luca Maria Sconfienza
Rotator cuff calcific tendinopathy (RCCT) is a very common condition caused by the presence of calcific deposits in the rotator cuff (RC) or in the subacromial-subdeltoid (SASD) bursa when calcification spreads around the tendons. The pathogenetic mechanism of RCCT is still unclear. It seems to be related to cell-mediated disease in which metaplastic transformation of tenocytes into chondrocytes induces calcification inside the tendon of the RC. RCCT is a frequent finding in the RC that may cause significant shoulder pain and disability...
January 19, 2018: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/29350537/cartilage-morphological-and-histological-findings-after-reconstruction-of-the-glenoid-with-an-iliac-crest-bone-graft
#4
Alexander Auffarth, Herbert Resch, Nicholas Matis, Martin Hudelmaier, Wolfgang Wirth, Rosemarie Forstner, Daniel Neureiter, Andreas Traweger, Philipp Moroder
BACKGROUND: The J-bone graft is presumably representative of iliac crest bone grafts in general and allows anatomic glenoid reconstruction in cases of bone defects due to recurrent traumatic anterior shoulder dislocations. As a side effect, these grafts have been observed to be covered by some soft, cartilage-like tissue when arthroscopy has been indicated after such procedures. PURPOSE: To evaluate the soft tissue covering of J-bone grafts by use of magnetic resonance imaging (MRI) and histological analysis...
January 1, 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29350420/over-expression-of-dec1-inhibits-myogenic-differentiation-by-modulating-myog-activity-in-bovine-satellite-cell
#5
Yongzhen Huang, Xinsheng Lai, Linyong Hu, Chuzhao Lei, Xianyong Lan, Chunlei Zhang, Yun Ma, Li Zheng, Yue-Yu Bai, Fengpeng Lin, Hong Chen
Differentiated embryo chondrocyte 1 (DEC1), a member of basic-helix-loop-helix transcription factor Bhlhe40, also called stimulated by retinoic acid 13, STRA13, plays an important role in the regulation of adipogenesis, tumorigenesis, peripheral circadian output, response to hypoxia and development of metabolic syndrome. Previous studies suggested that DEC1 was involved in skeletal muscle development; however, its precise role in myoblast differentiation has not been determined. In the present study, we showed that DEC1 expressed ubiquitously in different bovine tissues and was down-regulated in differentiated bovine satellite cells...
January 19, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29349457/effects-of-tetrahedral-dna-nanostructures-on-autophagy-in-chondrocytes
#6
Sirong Shi, Shiyu Lin, Yong Li, Tao Zhang, Xiaoru Shao, Taoran Tian, Tengfei Zhou, Qianshun Li, Yunfeng Lin
Tetrahedral DNA nanostructures (TDNs) have gathered great attention and are being widely used in biomedicine. We demonstrated that autophagy increased after exposure to TDNs (250 nM) along with the up-regulation of several autophagy-related genes and proteins. TDNs enhanced cell autophagy through the PI3K/AKT/mTOR signaling pathway.
January 19, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29348392/rapamycin-prevents-the-intervertebral-disc-degeneration-via-inhibiting-differentiation-and-senescence-of-annulus-fibrosus-cells
#7
Changhong Gao, Bin Ning, Chenglin Sang, Ying Zhang
The effects of bleomycin and rapamycin on cellular senescence and differentiation of rabbit annulus fibrosus stem cells (AFSCs) were investigated using a cell culture model. The results showed that bleomycin induced cellular senescence in AFSCs as evidenced by senescence-associated secretory phenotype. The morphology of AFSCs was changed from cobblestone-like cells to pancake-like cells. The senescence-associated β-galactosidase activity, the protein expression of P16 and P21, and inflammatory-related marker gene levels IL-1β, IL-6, and TNF-α were increased in bleomycin-treated AFSCs in a dose-dependent manner...
January 18, 2018: Aging
https://www.readbyqxmd.com/read/29346562/scaffold-free-trachea-regeneration-by-tissue-engineering-with-bio-3d-printing
#8
Daisuke Taniguchi, Keitaro Matsumoto, Tomoshi Tsuchiya, Ryusuke Machino, Yosuke Takeoka, Abdelmotagaly Elgalad, Kiyofumi Gunge, Katsunori Takagi, Yasuaki Taura, Go Hatachi, Naoto Matsuo, Naoya Yamasaki, Koichi Nakayama, Takeshi Nagayasu
OBJECTIVES: Currently, most of the artificial airway organs still require scaffolds; however, such scaffolds exhibit several limitations. Alternatively, the use of an autologous artificial trachea without foreign materials and immunosuppressants may solve these issues and constitute a preferred tool. The rationale of this study was to develop a new scaffold-free approach for an artificial trachea using bio-3D printing technology. Here, we assessed the circumferential tracheal replacement using scaffold-free trachea-like grafts generated from isolated cells in an inbred animal model...
January 15, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29346012/the-combination-of-probiotic-complex-rosavin-and-zinc-improves-pain-and-cartilage-destruction-in-an-osteoarthritis-rat-model
#9
Ji Ye Kwon, Seung Hoon Lee, JooYeon Jhun, JeongWon Choi, KyungAh Jung, Keun Hyung Cho, Seok Jung Kim, Chul Woo Yang, Sung-Hwan Park, Mi-La Cho
Osteoarthritis (OA), a degenerative disorder, induces pain, joint inflammation, and destruction of the articular cartilage matrix. Probiotic complex, rosavin, and zinc have been used as dietary supplements that exhibit anti-inflammatory and antioxidant properties. However, there is no evidence demonstrating a synergic effect in OA. This study aims to determine whether combination with probiotic complex, rosavin, and zinc decreases progression of monosodium iodoacetate (MIA)-induced OA rat model. The combination improved pain levels by preventing cartilage damage...
January 18, 2018: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29345353/hipop-mesenchymal-population-has-high-potential-for-repairing-injured-peripheral-nerves
#10
Yukako Yamauchi, Shousaku Itoh, Haruna Naruse, Yuki Itoh, Makoto Abe, Takumi Kagioka, Mikako Hayashi
Bone marrow stromal cells (BMSCs) are reportedly a heterogeneous population of mesenchymal stem cells (MSCs). Recently, we developed a simple strategy for the enrichment of MSCs with the capacity to differentiate into osteoblasts, chondrocytes, and adipocytes. On transplantation, the progenitor-enriched fractions can regenerate the bone with multiple lineages of donor origin and are thus called "highly purified osteoprogenitors" (HipOPs). Although our previous studies have demonstrated that HipOPs are enriched with MSCs and exhibit a higher potential to differentiate into osteoblasts, adipocytes, and chondrocytes than BMSCs, their potential to differentiate into neural cells has not been clarified...
January 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29344659/degree-of-endplate-chondrocyte-degeneration-in-different-tension-regions-during-mechanical-stimulation
#11
Yongming Xu, Shujuan Xu, Zhi Gao, Liang Xiao, Fei Xiao, Hongguang Xu, Xiaoling Zhang
The aim of this study was to explore the degree of degeneration of endplate chondrocytes in different tension regions induced by intermittent cyclic mechanical tension (ICMT) in vitro. Rat endplate chondrocytes were harvested and treated with 10% ICMT for 8 h/day with a frequency of 0.5 Hz. A cartilage degeneration model was induced using an FX‑5000T cell strain‑loading system. The experiment was divided into the central region and the peripheral region, according to the contact area between the loading post and the six‑well flexible silicone rubber BioFlex plates...
January 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29343853/differential-tissue-specific-temporal-and-spatial-expression-patterns-of-the-aggrecan-gene-is-modulated-by-independent-enhancer-elements
#12
Ian M H Li, Ke Liu, Alice Neal, Peter D Clegg, Sarah De Val, George Bou-Gharios
The transcriptional mechanism through which chondrocytes control the spatial and temporal composition of the cartilage tissue has remained largely elusive. The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue. We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation. Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29342317/osteoblast-like-mc3t3-e1-cells-prefer-glycolysis-for-atp-production-but-adipocyte-like-3t3-l1-cells-prefer-oxidative-phosphorylation
#13
Anyonya R Guntur, Akos A Gerencser, Phuong T Le, Victoria E DeMambro, Sheila A Bornstein, Shona A Mookerjee, David E Maridas, David E Clemmons, Martin D Brand, Clifford J Rosen
Mesenchymal stromal cells (MSCs) are early progenitors that can differentiate into osteoblasts, chondrocytes and adipocytes. We hypothesized that osteoblasts and adipocytes utilize distinct bioenergetic pathways during MSC differentiation. To test this hypothesis, we compared the bioenergetic profiles of pre-osteoblast MC3T3-E1 cells and calvarial osteoblasts with pre-adipocyte 3T3L1 cells, before and after differentiation. Differentiated MC3T3-E1 osteoblasts met ATP demand mainly by glycolysis with minimal reserve glycolytic capacity, whereas non-differentiated cells generated ATP through oxidative phosphorylation...
January 17, 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29339787/cilia-related-protein-spef2-regulates-osteoblast-differentiation
#14
Mari S Lehti, Henna Henriksson, Petri Rummukainen, Fan Wang, Liina Uusitalo-Kylmälä, Riku Kiviranta, Terhi J Heino, Noora Kotaja, Anu Sironen
Sperm flagellar protein 2 (SPEF2) is essential for motile cilia, and lack of SPEF2 function causes male infertility and primary ciliary dyskinesia. Cilia are pointing out from the cell surface and are involved in signal transduction from extracellular matrix, fluid flow and motility. It has been shown that cilia and cilia-related genes play essential role in commitment and differentiation of chondrocytes and osteoblasts during bone formation. Here we show that SPEF2 is expressed in bone and cartilage. The analysis of a Spef2 knockout (KO) mouse model revealed hydrocephalus, growth retardation and death prior to five weeks of age...
January 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29339093/the-proteoglycan-biglycan-mediates-inflammatory-response-by-activating-tlr-4-in-human-chondrocytes-inhibition-by-specific-sirna-and-high-polymerized-hyaluronan
#15
Angela Avenoso, Angela D'Ascola, Michele Scuruchi, Giuseppe Mandraffino, Alberto Calatroni, Antonino Saitta, Salvatore Campo, Giuseppe M Campo
Cartilage degeneration are hallmarks of wear, tear, mechanical and inflammatory damage of the joint cartilage. Tissue degradation as well as compromising the integrity and function of the organ, produces different intermediates, directly able to stimulate further inflammatory effect, therefore, amplifying the inflammation response. Biglycan is a soluble component of the extracellular matrix that is released during tissue injury. It has been reported that released biglycan is an endogenous ligand for TLR-2/4 in some cell type...
January 12, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29339024/nrf2-are-pathway-attenuates-oxidative-and-apoptotic-response-in-human-osteoarthritis-chondrocytes-by-activating-erk1-2-elk1-p70s6k-p90rsk-signaling-axis
#16
Nazir M Khan, Imran Ahmad, Tariq M Haqqi
Nrf2, a redox regulated transcription factor, has recently been shown to play a role in cartilage integrity but the mechanism remains largely unknown. Osteoarthritis (OA) is a multifactorial disease in which focal degradation of cartilage occurs. Here, we studied whether Nrf2 exerts chondroprotective effects by suppressing the oxidative stress and apoptosis in IL-1β stimulated human OA chondrocytes. Expression of Nrf2 and its target genes HO-1, NQO1 and SOD2 was significantly high in OA cartilage compared to normal cartilage and was also higher in damaged area compared to smooth area of OA cartilage of the same patient...
January 12, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29335505/perk-mediated-translational-control-is-required-for-collagen-secretion-in-chondrocytes
#17
Satoshi Hisanaga, Masato Miyake, Shusuke Taniuchi, Miho Oyadomari, Masatoshi Morimoto, Ryosuke Sato, Jun Hirose, Hiroshi Mizuta, Seiichi Oyadomari
As chondrocytes are highly secretory and they experience a variety of stresses, physiological unfolded protein response (UPR) signalling is essential for extracellular matrix (ECM) secretion and chondrogenesis. In the three branches of the UPR pathway, PERK governs the translational attenuation and transcriptional upregulation of amino acid and redox metabolism and induction of apoptosis. It was previously demonstrated that a defect of the PERK branch of the UPR signalling pathway causes the accumulation of unfolded proteins, leading to cell death without perturbing endoplasmic reticulum (ER)-to-Golgi transport in pancreatic β cells...
January 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29334399/chondrocyte-burst-promotes-space-for-mineral-expansion
#18
Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Letycia Mary Iida, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto
Analysis of tissue development from multidisciplinary approaches can result in more integrative biological findings, and can eventually allow the development of more effective bioengineering methods. In this study, we analyzed the initial steps of mineral formation during secondary ossification of mouse femur based on biological and bioengineering approaches. We first found that some chondrocytes burst near the mineralized area. External factors that could trigger chondrocyte burst were then investigated. Chondrocyte burst was shown to be modulated by mechanical and osmotic pressure...
January 15, 2018: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29334180/nitric-oxide-dependent-degradation-of-polyethylene-glycol-modified-single-walled-carbon-nanotubes-implications-for-intra-articular-delivery
#19
Kunal Bhattacharya, Cristiano Sacchetti, Pedro M Costa, Jens Sommertune, Birgit D Brandner, Andrea Magrini, Nicola Rosato, Nunzio Bottini, Massimo Bottini, Bengt Fadeel
Polyethylene glycol (PEG)-modified carbon nanotubes have been successfully employed for intra-articular delivery in mice without systemic or local toxicity. However, the fate of the delivery system itself remains to be understood. In this study 2 kDa PEG-modified single-walled carbon nanotubes (PNTs) are synthesized, and trafficking and degradation following intra-articular injection into the knee-joint of healthy mice are studied. Using confocal Raman microspectroscopy, PNTs can be imaged in the knee-joint and are found to either egress from the synovial cavity or undergo biodegradation over a period of 3 weeks...
January 15, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29331857/baicalin-alleviates-il-1%C3%AE-induced-inflammatory-injury-via-down-regulating-mir-126-in-chondrocytes
#20
Xirui Yang, Qi Zhang, Zhaomeng Gao, Chunyan Yu, Lei Zhang
Baicalin is a flavonoid extracted from Scutellaria baicalensis Georgi, with anti-inflammatory and anti-apoptotic activities. The objective of this study was to explore the effect and mechanism of baicalin on chondrocyte inflammatory response in OA. Different concentrations of IL-1β (0, 0.1, 2, 5 and 10 ng/mL) were used to simulate inflammatory injury in CHON-001 cells. The expression of miR-126 was altered by transfection with miR-126 mimic. Thereafter, cells were treated with baicalin, and cell viability, apoptosis, the expressions of apoptosis-related protein and pro-inflammatory factors were respectively detected using CCK-8 assay, flow cytometry, qRT-PCR and western blot analysis...
January 11, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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