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https://www.readbyqxmd.com/read/28221968/isolation-identification-and-characterization-of-foodborne-pathogens-isolated-from-egg-internal-contents-in-china
#1
Zhao Ge, Song Xue, Zhao Jianmei, L I Yuehua, Wang Juan, Huang Xiumei, Q U Zhina, Wang Yudong, Yan Shigan, Wang Junwei
Eggs continue to be significant in terms of the world economy and human nutrition. The aim of this study was to estimate the prevalence of foodborne pathogens in a cross section of egg production types in China and to examine the virulence features of the isolated pathogens. Three hundred eggs from three provinces were tested for Salmonella , Escherichia coli , Staphylococcus aureus , and Campylobacter . Two eggs were positive for Salmonella , one was positive for both E. coli and S. aureus , and none were positive for Campylobacter ...
December 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221965/explanatory-variables-associated-with-campylobacter-and-escherichia-coli-concentrations-on-broiler-chicken-carcasses-during-processing-in-two-slaughterhouses
#2
Ewa Pacholewicz, Arno Swart, Jaap A Wagenaar, Len J A Lipman, Arie H Havelaar
This study aimed at identifying explanatory variables that were associated with Campylobacter and Escherichia coli concentrations throughout processing in two commercial broiler slaughterhouses. Quantative data on Campylobacter and E. coli along the processing line were collected. Moreover, information on batch characteristics, slaughterhouse practices, process performance, and environmental variables was collected through questionnaires, observations, and measurements, resulting in data on 19 potential explanatory variables...
December 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221919/variation-in-campylobacter-multilocus-sequence-typing-subtypes-from-chickens-as-detected-on-three-plating-media
#3
M E Berrang, S R Ladely, R J Meinersmann, J E Line, B B Oakley, N A Cox
The objective of this study was to compare subtypes of Campylobacter jejuni and Campylobacter coli detected on three selective Campylobacter plating media to determine whether each medium selected for different subtypes. Fifty ceca and 50 carcasses (representing 50 flocks) were collected from the evisceration line in a commercial broiler processing plant. Campylobacter was cultured and isolated from cecal contents and carcass rinses on Campy-Cefex, Campy Line, and RF Campylobacter jejuni/coli agars. When a positive result was obtained with all three media, one colony of the most prevalent morphology on each medium was selected for further analysis by full genome sequencing and multilocus sequence typing...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221913/an-immunoassay-for-quantification-of-contamination-by-raw-meat-juice-on-food-contact-surfaces
#4
Fur-Chi Chen, Sandria Godwin, Edgar Chambers
Raw chicken products often are contaminated with Salmonella and Campylobacter , which can be transmitted from packages to contact surfaces. Raw meat juices from these packages also provide potential media for cross-contamination. There are limited quantitative data on the levels of consumer exposure to raw meat juice during shopping for and handling of chicken products. An exposure assessment is needed to quantify the levels of transmission and to assess the risk. An enzyme-linked immunosorbent assay (ELISA) was developed and validated for quantitative detection of raw meat juice on hands and various food contact surfaces...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221911/molecular-subtyping-and-source-attribution-of-campylobacter-isolated-from-food-animals
#5
Gregory H Tyson, Heather P Tate, Jason Abbott, Thu-Thuy Tran, Claudine Kabera, Emily Crarey, Shenia Young, Patrick F McDERMOTT, Grisselle Sprague, Mark Campbell, Oyewole Adeyemo, Johnette Browne-Silva, Michael Myers, Sutawee Thitaram, Shaohua Zhao
Campylobacter spp. commonly cause gastrointestinal illness in humans. Poultry meats have long been considered the predominant source of these infections, but few in-depth Campylobacter source attribution studies have been completed. We analyzed more than 1,300 Campylobacter isolates recovered from a number of animal and food sources, including dairy and beef cattle, pigs, poultry, and retail poultry meat, and compared them with Campylobacter isolates recovered from human clinical samples. Each isolate was subtyped using pulsed-field gel electrophoresis (PFGE) with SmaI and queried against the Centers for Disease Control and Prevention PulseNet database to identify human isolates with indistinguishable patterns...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221899/improvement-of-karmali-agar-by-supplementation-with-tazobactam-for-detecting-campylobacter-in-raw-poultry
#6
Young-Ji Kim, Chon-Jung Whan, Hong-Seok Kim, Kwang-Yeop Kim, Jin-Hyeok Yim, Seung-Hak Cho, Kun-Ho Seo
In this study, Karmali agar was modified by adding tazobactam (T-Karmali agar) to suppress the growth of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli , which frequently contaminates raw poultry meat. By inoculating 30 Campylobacter spp. strains and 25 ESBL-producing E. coli strains onto Karmali agar and T-Karmali agar containing various concentrations of the antibacterial agent, we determined the optimum concentration of tazobactam to be 4 mg/liter. The Campylobacter spp. isolation rate on T-Karmali agar (13...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221843/campylobacter-spp-prevalence-and-levels-in-raw-milk-a-systematic-review-and-meta-analysis
#7
T Christidis, K D M Pintar, A J Butler, A Nesbitt, M K Thomas, B Marshall, F Pollari
Campylobacteriosis is the leading bacterial gastrointestinal disease internationally, contributing significantly to the enteric illness burden. Cases have been associated with the consumption of raw milk, a behavior that has garnered attention recently. Estimates of the prevalence and levels of Campylobacter spp. in raw milk are lacking, which hinders risk assessment attempts. This article is a systematic review and meta-analysis of reported prevalence and levels of zoonotic Campylobacter spp. in the raw milk of cows, goats, and sheep in Canada, the United States, Europe, Australia, and New Zealand...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28220790/in-vivo-genome-editing-with-a-small-cas9-orthologue-derived-from-campylobacter-jejuni
#8
Eunji Kim, Taeyoung Koo, Sung Wook Park, Daesik Kim, Kyoungmi Kim, Hee-Yeon Cho, Dong Woo Song, Kyu Jun Lee, Min Hee Jung, Seokjoong Kim, Jin Hyoung Kim, Jeong Hun Kim, Jin-Soo Kim
Several CRISPR-Cas9 orthologues have been used for genome editing. Here, we present the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni (CjCas9), for efficient genome editing in vivo. After determining protospacer-adjacent motif (PAM) sequences and optimizing single-guide RNA (sgRNA) length, we package the CjCas9 gene, its sgRNA sequence, and a marker gene in an all-in-one adeno-associated virus (AAV) vector and produce the resulting virus at a high titer. CjCas9 is highly specific, cleaving only a limited number of sites in the human or mouse genome...
February 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28217442/whole-genome-sequencing-and-analysis-of-campylobacter-coli-yh502-from-retail-chicken-reveals-a-plasmid-borne-type-vi-secretion-system
#9
Sandeep Ghatak, Yiping He, Sue Reed, Terence Strobaugh, Peter Irwin
Campylobacter is a major cause of foodborne illnesses worldwide. Campylobacter infections, commonly caused by ingestion of undercooked poultry and meat products, can lead to gastroenteritis and chronic reactive arthritis in humans. Whole genome sequencing (WGS) is a powerful technology that provides comprehensive genetic information about bacteria and is increasingly being applied to study foodborne pathogens: e.g., evolution, epidemiology/outbreak investigation, and detection. Herein we report the complete genome sequence of Campylobacter coli strain YH502 isolated from retail chicken in the United States...
March 2017: Genomics Data
https://www.readbyqxmd.com/read/28213012/evaluating-and-improving-terminal-hygiene-practices-on-broiler-farms-to-prevent-campylobacter-cross-contamination-between-flocks
#10
Tara Battersby, D Walsh, P Whyte, D Bolton
The objectives of this study were to evaluate current cleaning practices in broiler houses by testing a range of sites after cleaning and disinfection and to test the efficacy of the most commonly used methods in a commercial broiler house after flock harvesting. Cleaning procedures on 20 broiler houses (10 separate farms) were examined by testing a range of sampling points (feeders, drinkers, walls, etc.) for total viable count (TVC), total Enterobacteriaceae count (TEC) and Campylobacter spp. after cleaning and disinfection, using culture based methods...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28208612/enteric-pathogens-and-their-toxin-induced-disruption-of-the-intestinal-barrier-through-alteration-of-tight-junctions-in-chickens
#11
REVIEW
Wageha A Awad, Claudia Hess, Michael Hess
Maintaining a healthy gut environment is a prerequisite for sustainable animal production. The gut plays a key role in the digestion and absorption of nutrients and constitutes an initial organ exposed to external factors influencing bird's health. The intestinal epithelial barrier serves as the first line of defense between the host and the luminal environment. It consists of a continuous monolayer of intestinal epithelial cells connected by intercellular junctional complexes which shrink the space between adjacent cells...
February 10, 2017: Toxins
https://www.readbyqxmd.com/read/28205571/erratum-genome-analysis-of-campylobacter-concisus-strains-from-patients-with-inflammatory-bowel-disease-and-gastroenteritis-provides-new-insights-into-pathogenicity
#12
Heung Kit Leslie Chung, Alfred Tay, Sophie Octavia, Jieqiong Chen, Fang Liu, Rena Ma, Ruiting Lan, Stephen M Riordan, Michael C Grimm, Li Zhang
No abstract text is available yet for this article.
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28202878/campylobacter-upsaliensis-isolated-from-dog-produces-high-titer-of-cytolethal-distending-toxin
#13
Srinuan Somroop, Noritoshi Hatanaka, Sharda Prasad Awasthi, Kentaro Okuno, Masahiro Asakura, Atsushi Hinenoya, Shinji Yamasaki
Cytolethal distending toxin (CDT) consisting of CdtA, CdtB and CdtC has been reported to be a possible virulence factor of campylobacters including C. upsaliensis. In our previous study, the cdtB gene-based PCR-restriction fragment length polymorphisms (RFLP) assay for detection and differentiation of 7 Campylobacter species yielded 3 different RFLP patterns (Cu-I to Cu-III). In this study, entire cdt (Cucdt) genes of each pattern were sequenced to see whether there are any differences in cdt genes, its amino acid sequences and biological activity of CuCDT...
February 16, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28202797/the-epiquant-framework-for-computing-the-epidemiological-concordance-of-microbial-subtyping-data
#14
Benjamin M Hetman, Steven K Mutschall, James E Thomas, Victor P J Gannon, Clifford G Clark, Frank Pollari, Eduardo N Taboada
A fundamental assumption in the use and interpretation of microbial subtyping results for public health investigations is that isolates that appear to be related based on molecular subtyping data are expected to share commonalities with respect to their origin, history and distribution. Critically, no approach currently exists for systematically assessing the underlying epidemiology of subtyping results. Our aim was to develop a method for directly quantifying the similarity between bacterial isolates using basic sampling metadata and to develop a framework for computing the epidemiologic concordance of microbial typing results...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28201757/oxidative-and-nitrosative-stress-defences-of-helicobacter-and-campylobacter-species-that-counteract-mammalian-immunity
#15
Annika Flint, Alain Stintzi, Lígia M Saraiva
No abstract text is available yet for this article.
November 1, 2016: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/28196097/temporal-induction-of-pro-inflammatory-and-regulatory-cytokines-in-human-peripheral-blood-mononuclear-cells-by-campylobacter-jejuni-and-campylobacter-coli
#16
Eman Hamza, Sonja Kittl, Peter Kuhnert
Campylobacter jejuni along with C. coli are major cause of human gastroenteritis worldwide. So far, the human immune response against Campylobacter is not entirely clear. We hypothesize that it is coordinated by an interaction between pro-inflammatory and regulatory cytokines which is influenced by bacterial and host-individual differences. Accordingly, we used peripheral blood mononuclear cells (PBMC) from healthy donors to study the primary systemic immune response to C. jejuni and C. coli. PBMC were stimulated by different strains of C...
2017: PloS One
https://www.readbyqxmd.com/read/28194914/genetic-diversity-and-antimicrobial-resistance-profiles-of-campylobacter-coli-and-campylobacter-jejuni-isolated-from-broiler-chicken-in-farms-and-at-slaughter-in-central-italy
#17
S Pergola, M P Franciosini, F Comitini, M Ciani, S De Luca, S Bellucci, L Menchetti, P Casagrande Proietti
AIMS: Genetic diversity and antimicrobial resistance of C. coli and C. jejuni were investigated along the broiler chicken production chain in central Italy. METHODS AND RESULTS: Campylobacter spp. isolated from cloacal swabs in farms (n = 116) and from neck skin of chilled and eviscerated carcasses at slaughter (n = 24) were identified as C. coli (n = 99) and C. jejuni (n = 41) by multiplex PCR. Characterisation by s-AFLP revealed a specific genotype of Campylobacter for each farm...
February 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28194411/antibiotic-resistance-of-campylobacter-recovered-from-faeces-and-carcasses-of-healthy-livestock
#18
Akosua B Karikari, Kwasi Obiri-Danso, Enoch H Frimpong, Karen A Krogfelt
Campylobacter is of major significance in food safety and human and veterinary medicine. This study highlighted resistance situation in the area of veterinary public health in Ghana. Using selective mCCDA agar, isolates were confirmed phenotypically on API CAMPY and genotypically by multiplex PCR of IpxA gene. The susceptibility profile of species to common and relevant antibiotics was determined by the Kirby-Bauer disk diffusion method. Cattle, sheep, goat, and pig faecal samples analysed, respectively, yielded 13...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28190406/an-assessment-of-the-microbiological-quality-of-liver-based-p%C3%A3-t%C3%A3-in-england-2012-13-comparison-of-samples-collected-at-retail-and-from-catering-businesses
#19
J McLAUCHLIN, F Jørgensen, H Aird, A Charlett, N Elviss, D Fenelon, A Fox, C Willis, C F L Amar
The purpose of this study was to investigate the microbiological quality of liver pâté. During 2012-13, a total of 870 samples, unrelated to the investigation of food-poisoning outbreaks, were collected either at retail (46%), catering (53%) or the point of manufacture (1%) and were tested using standard methods to detect Salmonella spp. or Campylobacter spp., and to enumerate for Listeria spp., including Listeria monocytogenes, Clostridium perfringens, coagulase-positive staphylococci including Staphylococcus aureus, Bacillus spp...
February 13, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28190368/rectal-bleeding-in-neonates-due-to-campylobacter-enteritis
#20
Jessica Hirschel, Denise Herzog, Gregor W Kaczala
No abstract text is available yet for this article.
February 1, 2017: Clinical Pediatrics
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