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https://www.readbyqxmd.com/read/28646425/hydroxylamine-induced-oxidation-of-ferrous-carbonylated-truncated-hemoglobins-from-mycobacterium-tuberculosis-and-campylobacter-jejuni-is-limited-by-carbon-monoxide-dissociation
#1
Paolo Ascenzi, Chiara Ciaccio, Tecla Gasperi, Alessandra Pesce, Lucia Caporaso, Massimo Coletta
Hydroxylamine (HA) is an oxidant of ferrous globins and its action has been reported to be inhibited by CO, even though this mechanism has not been clarified. Here, kinetics of the HA-mediated oxidation of ferrous carbonylated Mycobacterium tuberculosis truncated hemoglobin N and O (Mt-trHbN(II)-CO and Mt-trHbO(II)-CO, respectively) and Campylobacter jejuni truncated hemoglobin P (Cj-trHbP(II)-CO), at pH 7.2 and 20.0 °C, are reported. Mixing Mt-trHbN(II)-CO, Mt-trHbO(II)-CO, and Cj-trHbP(II)-CO solution with the HA solution brings about absorption spectral changes reflecting the disappearance of the ferrous carbonylated derivatives with the concomitant formation of the ferric species...
June 23, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28646056/campylobacter-levels-in-fresh-chickens-down-on-2016-retailer-survey-shows
#2
(no author information available yet)
No abstract text is available yet for this article.
June 24, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28642840/cytolethal-distending-toxin-enhances-radiosensitivity-in-prostate-cancer-cells-by-regulating-autophagy
#3
Hwai-Jeng Lin, Hsin-Ho Liu, Chia-Der Lin, Min-Chuan Kao, Yu-An Chen, Chuan Chiang-Ni, Zhi-Pei Jiang, Mei-Zi Huang, Chun-Jung Lin, U-Ging Lo, Li-Chiung Lin, Cheng-Kuo Lai, Ho Lin, Jer-Tsong Hsieh, Cheng-Hsun Chiu, Chih-Ho Lai
Cytolethal distending toxin (CDT) produced by Campylobacter jejuni contains three subunits: CdtA, CdtB, and CdtC. Among these three toxin subunits, CdtB is the toxic moiety of CDT with DNase I activity, resulting in DNA double-strand breaks (DSB) and, consequently, cell cycle arrest at the G2/M stage and apoptosis. Radiation therapy is an effective modality for the treatment of localized prostate cancer (PCa). However, patients often develop radioresistance. Owing to its particular biochemical properties, we previously employed CdtB as a therapeutic agent for sensitizing radioresistant PCa cells to ionizing radiation (IR)...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28640123/recurrent-campylobacter-jejuni-bacteremia-in-a-patient-with-hypogammaglobulinemia-a-case-report
#4
Youie Kim, Ju Ae Shin, Seung Beom Han, Bin Cho, Dae Chul Jeong, Jin Han Kang
RATIONALE: Although some cases of recurrent bacteremia due to Campylobacter jejuni have been reported in immunocompromised patients, antibiotic treatment strategies to eradicate C. jejuni and prevent recurrent infections in immunocompromised patients have not been established. Authors' experience of such rare cases should be shared for improving patients' outcomes. PATIENT CONCERNS: An 18-year-old boy with hypogammaglobulinemia, who received intravenous immunoglobulin replacement therapy every 3 weeks, was admitted to hospital repeatedly due to recurrent diarrhea and cellulitis of the leg...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28636341/biochemical-characterization-of-wbkc-an-n-formyltransferase-from-brucella-melitensis
#5
Alexander S Riegert, Daniel P Chantigian, James B Thoden, Peter A Tipton, Hazel M Holden
It has become increasingly apparent within the last several years that unusual N-formylated sugars are often found on the O-antigens of such Gram negative pathogenic organisms as Francisella tularensis, Campylobacter jejuni, and Providencia alcalifaciens, amongst others. Indeed, in some species of Brucella, for example, the O-antigen contains 1,2-linked 4-formamido-4,6-dideoxy-alpha-D-mannosyl groups. These sugars, often referred to as N-formylperosamine, are synthesized in pathways initiating with GDP-mannose...
June 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28634541/campylobacter-jejuni-myocarditis-a-journey-from-the-gut-to-the-heart
#6
M Toba Obafemi, Homeyra Douglas
OBJECTIVES: Campylobacter jejuni is an unusual cause of myocarditis and could easily be missed. METHODS: We describe a case of a 25 year old man, who presented with 3 day history of vomiting and diarrhoea, followed by chest pain and significant high sensitive troponin rise. RESULTS: The patient's profuse diarrhoea was accompanied by raised inflammatory markers, electrocardiogram changes and evidence of cardiomyopathy on transthoracic echocardiogram...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28633450/comparative-genomics-of-all-three-campylobacter-sputorum-biovars-and-a-novel-cattle-associated-c-sputorum-clade
#7
William G Miller, Emma Yee, Mary H Chapman, James L Bono
Campylobacter sputorum is a non-thermotolerant campylobacter that is primarily isolated from food animals such as cattle and sheep. C. sputorum is also infrequently associated with human illness. Based on catalase and urease activity, three biovars are currently recognized within C. sputorum: bv. sputorum (catalase negative, urease negative), bv. fecalis (catalase positive, urease negative), and bv. paraureolyticus (catalase negative, urease positive). A multi-locus sequence typing (MLST) method was recently constructed for C...
June 19, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28630348/analysis-of-a-dual-domain-phosphoglycosyl-transferase-reveals-a-ping-pong-mechanism-with-a-covalent-enzyme-intermediate
#8
Debasis Das, Petr Kuzmic, Barbara Imperiali
Phosphoglycosyl transferases (PGTs) are integral membrane proteins with diverse architectures that catalyze the formation of polyprenol diphosphate-linked glycans via phosphosugar transfer from a nucleotide diphosphate-sugar to a polyprenol phosphate. There are two PGT superfamilies that differ significantly in overall structure and topology. The polytopic PGT superfamily, represented by MraY and WecA, has been the subject of many studies because of its roles in peptidoglycan and O-antigen biosynthesis. In contrast, less is known about a second, extensive superfamily of PGTs that reveals a core structure with dual domain architecture featuring a C-terminal soluble globular domain and a predicted N-terminal membrane-associated domain...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28629878/campylobacter-bacteremia-in-london-a-44-year-single-center-study
#9
Geraldine A O'Hara, Joseph R A Fitchett, John L Klein
PURPOSE: Campylobacter species are a well-recognized but rare cause of bloodstream infection. METHODS: Here we reviewed 41 cases of Campylobacter bloodstream infection occurring at a single center in London over 44years, comprising 0.2% of all recorded episodes during this time period. RESULTS: Patients had a mean age of 46years and, contrasting with previous reports, nearly 50% of our patients did not have significant comorbidities. Ciprofloxacin resistance increased over the study period with 35% of isolates overall being resistant compared with only 3% exhibiting macrolide resistance...
June 2, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28629508/campylobacter-pinnipediorum-sp-nov-isolated-from-pinnipeds-comprising-campylobacter-pinnipediorum-subsp-pinnipediorum-subsp-nov-and-campylobacter-pinnipediorum-subsp-caledonicus-subsp-nov
#10
Maarten J Gilbert, William G Miller, Judy St Leger, Mary H Chapman, Arjen J Timmerman, Birgitta Duim, Geoffrey Foster, Jaap A Wagenaar
During independent diagnostic screenings of otariid seals in California (USA) and phocid seals in Scotland (UK), Campylobacter-like isolates, which differed from the established taxa of the genus Campylobacter, were cultured from abscesses and internal organs of different seal species. A polyphasic study was undertaken to determine the taxonomic position of these six isolates. The isolates were characterized by 16S rRNA gene and AtpA sequence analysis and by conventional phenotypic testing. The whole-genome sequences were determined for all isolates, and the average nucleotide identity (ANI) was determined...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28628752/high-prevalence-of-resistance-to-fluoroquinolones-and-tetracycline-campylobacter-spp-isolated-from-poultry-in-poland
#11
Anna Woźniak-Biel, Gabriela Bugla-Płoskońska, Alicja Kielsznia, Kamila Korzekwa, Anna Tobiasz, Agnieszka Korzeniowska-Kowal, Alina Wieliczko
Campylobacter spp. is a major cause of foodborne diseases in humans, particularly when transmitted by the handling or consumption of undercooked poultry meat. Most Campylobacter infections are self-limiting, but antimicrobial treatment (e.g., fluoroquinolones and macrolides) is necessary in severe or prolonged cases. The indiscriminate use of these drugs, both in clinical medicine and animal production, has a major impact on public health. The aim of the present study was to identify Campylobacter strains, isolated from turkey and broilers, using both PCR and the matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) methods to reveal the accuracy of identification, as well to evaluate the antimicrobial and genetic resistance of the investigated strains...
June 19, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28627855/multiplex-pcr-gastro-intestinal-panels-for-the-management-of-traveller-s-diarrhea-are-they-accurate-and-useful
#12
Laurence Rochat, Antony Croxatto, Serge De Vallière, Valérie D'Acremont, Blaise Genton
Until recently, the search for enteropathogens causing travellers' diarrhea was based on stool culture (Campylobacter spp., Salmonella spp. and Shigella spp.), direct microscopy with (Cryptosporidium spp.) or without specific staining (Giardia lamblia, Entamoeba histolytica) or specific antigen detection (Giardia lamblia, Entamoeba histolytica). Molecular analyses are progressively replacing traditional diagnostic methods but their clinical usefulness remains to be better defined. This article attempts to describe the advantages and disadvantages of these new molecular methods and to illustrate situations where they could be useful using clinical cases frequently encountered in the practice of travel medicine...
May 3, 2017: Revue Médicale Suisse
https://www.readbyqxmd.com/read/28627052/influence-of-different-protein-sources-in-the-broiler-diet-on-the-presence-of-campylobacter-spp-in-excreta-and-caecal-content
#13
C F Visscher, A Abd El-Wahab, M F E Ahmed, J Hankel, V Taube, J Kamphues
The aim of the present study was to examine possible effects of a different protein supply on the presence of Campylobacter in excreta and caecal content of broiler chickens. Therefore, 220 one-day-old chickens were fed five different diets in the experimental period (day 8 onwards). In the control group (CON; n = 3 boxes), a soya bean-based industrially produced complete diet containing 5-10% whole wheat and coccidiostats was fed, whereas in the remaining groups, four own-made complete diets with four protein sources and combinations (4 × 2 boxes; soya bean meal-based diet = SBM; rapeseed meal-containing diet = RSM; haemoglobin meal-containing diet = HGP; algae-containing diet = ALG) were offered...
June 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28623550/antibiotic-susceptibility-profiles-among-campylobacter-isolates-obtained-from-international-travelers-between-2007-and-2014
#14
A Post, D Martiny, N van Waterschoot, M Hallin, U Maniewski, E Bottieau, M Van Esbroeck, E Vlieghe, S Ombelet, O Vandenberg, J Jacobs
Campylobacter infection is a common cause of diarrhea among international travelers. We studied antibiotic resistance patterns among Campylobacter isolates obtained from international travelers according to travel destination. Three collections of isolates obtained from international travelers between 2007 and 2014 (Institute of Tropical Medicine, the "Laboratoire Hospitalier Universitaire de Bruxelles "and the Belgian National Reference Centre for Campylobacter) were used. Isolates were tested for minimal inhibitory concentration (MIC) values (E-test macromethod) for fluoroquinolones, macrolides, tetracyclines, amoxicillin-clavulanic acid, and meropenem...
June 17, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28622473/nonculturability-might-underestimate-the-occurrence-of-campylobacter-in-broiler-litter
#15
Issmat I Kassem, Yosra A Helmy, Dipak Kathayat, Rosario A Candelero-Rueda, Anand Kumar, Loic Deblais, Huang-Chi Huang, Orhan Sahin, Qijing Zhang, Gireesh Rajashekara
We investigated the contribution of litter to the occurrence of Campylobacter on three broiler farms, which were known to have low (LO) and high (HI-A and HI-B) Campylobacter prevalence. For this purpose, we collected litter samples (n = 288) during and after two rearing cycles from each farm. We evaluated the occurrence of Campylobacter (using selective enrichment and quantitative real-time polymerase chain reaction [q-PCR] analysis) in the litter samples as well as the litter's pH and moisture content...
June 16, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28621455/addition-of-rifampicin-to-bolton-broth-to-inhibit-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-for-the-detection-of-campylobacter
#16
Jung-Whan Chon, Young-Ji Kim, Young-Jo Kim, Ji Young Jung, Dongryeoul Bae, Saeed Khan, Kun-Ho Seo, Kidon Sung
Exponential growth of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in Campylobacter media has become a common problem for the detection of Campylobacter in chicken meats. We investigated the minimum inhibitory concentration of 40 ESBL-producing E. coli isolates from meats obtained from various countries against antibacterial agents in Bolton broth (cefoperazone, vancomycin, and trimethoprim). All ESBL-producing E. coli strains were resistant to cefoperazone and vancomycin, whereas 50% of them were resistant to trimethoprim and grew in Bolton broth...
June 16, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28619810/complete-genome-sequence-of-the-campylobacter-cuniculorum-type-strain-lmg-24588
#17
William G Miller, Emma Yee, Joana Revez, James L Bono, Mirko Rossi
Campylobacter cuniculorum is a thermotolerant species isolated from farmed rabbits (Oryctolagus cuniculus). Although C. cuniculorum is highly prevalent in rabbits farmed for human consumption, the pathogenicity of this organism in humans is still unknown. This study describes the whole-genome sequence of the C. cuniculorum type strain LMG 24588 (=CCUG 56289(T)).
June 15, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28619796/draft-genome-sequence-of-campylobacter-jejuni-cam970-and-c-%C3%A2-coli-cam962-associated-with-a-large-outbreak-of-foodborne-illness-in-fukuoka-japan-in-2016
#18
Hiroshi Asakura, Naoto Takahashi, Shiori Yamamoto, Hiroyuki Maruyama
Here, we report the draft genome sequences of Campylobacter jejuni CAM970 and C. coli CAM962, which were associated with a large outbreak of foodborne illness originating from undercooked chicken sushi in Fukuoka, Japan, in May 2016. Their genome sizes were 1,690,901 and 1,704,736 bp, with 22 and 23 rRNAs, 9 and 9 tRNAs, and 411× and 419× coverage for C. jejuni CAM970 and C. coli CAM962, respectively.
June 15, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28619636/isolation-and-characterization-of-a-non-specific-lipid-transfer-protein-from-chelidonium-majus-l-latex
#19
Robert Nawrot, Damian Jozefiak, Anna Sip, Dorota Kuzma, Oskar Musidlak, Anna Gozdzicka-Jozefiak, Jakub Barylski
Plant non-specific lipid transfer proteins (nsLTPs) are small basic proteins, which mostly play a role in intracellular lipid transport and antimicrobial defense. Recently it was shown using shotgun proteomic approach that the whole plant extract of Chelidonium majus L. (Papaveraceae) contains relatively abundant nsLTPs. Therefore the aim of the work was to isolate and characterize nsLTP from C. majus latex. RESULTS: obtained using PCR approach with degenerate primers showed nsLTP protein from C...
June 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28619013/mlst-genotypes-of-campylobacter-jejuni-isolated-from-broiler-products-dairy-cattle-and-human-campylobacteriosis-cases-in-lithuania
#20
Sigita Ramonaite, Egle Tamuleviciene, Thomas Alter, Neringa Kasnauskyte, Mindaugas Malakauskas
BACKGROUND: Campylobacter (C.) jejuni is the leading cause of human campylobacteriosis worldwide. We performed a molecular epidemiological study to investigate the genetic relationship among C. jejuni strains isolated from human diarrhoeal patients, broiler products and dairy cattle in Lithuania. METHODS: The C. jejuni isolates from human clinical cases, dairy cattle and broiler products were genotyped using multilocus sequence typing (MLST). Allele numbers for each housekeeping gene, sequence type (ST), and clonal complex (CC) were assigned by submitting the DNA sequences to the C...
June 15, 2017: BMC Infectious Diseases
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