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https://www.readbyqxmd.com/read/28212593/micrornas-and-endometriosis-distinguishing-drivers-from-passengers-in-disease-pathogenesis
#1
Warren B Nothnick
Endometriosis is a disease common in women of reproductive age, characterized by pelvic pain and infertility. Despite its prevalence, the factors and mechanisms which contribute to the development and survival of ectopic lesions remain uncertain. MicroRNAs (miRNAs) are small RNA molecules that regulate posttranscriptional gene regulation which have been proposed to contribute to the pathogenesis of many diseases including that of endometriosis. This review summarizes the results of initial studies describing differentially expressed miRNAs between endometriotic lesion tissue and eutopic endometrium...
February 17, 2017: Seminars in Reproductive Medicine
https://www.readbyqxmd.com/read/28212419/evaluating-the-lexico-grammatical-differences-in-the-writing-of-native-and-non-native-speakers-of-english-in-peer-reviewed-medical-journals-in-the-field-of-pediatric-oncology-creation-of-the-genuine-index-scoring-system
#2
Alberto Alexander Gayle, Motomu Shimaoka
INTRODUCTION: The predominance of English in scientific research has created hurdles for "non-native speakers" of English. Here we present a novel application of native language identification (NLI) for the assessment of medical-scientific writing. For this purpose, we created a novel classification system whereby scoring would be based solely on text features found to be distinctive among native English speakers (NS) within a given context. We dubbed this the "Genuine Index" (GI). METHODOLOGY: This methodology was validated using a small set of journals in the field of pediatric oncology...
2017: PloS One
https://www.readbyqxmd.com/read/28208500/sign-changes-as-a-universal-concept-in-first-passage-time-calculations
#3
Wilhelm Braun, Rüdiger Thul
First-passage-time problems are ubiquitous across many fields of study, including transport processes in semiconductors and biological synapses, evolutionary game theory and percolation. Despite their prominence, first-passage-time calculations have proven to be particularly challenging. Analytical results to date have often been obtained under strong conditions, leaving most of the exploration of first-passage-time problems to direct numerical computations. Here we present an analytical approach that allows the derivation of first-passage-time distributions for the wide class of nondifferentiable Gaussian processes...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28203521/down-regulation-of-mir-26b-induces-cisplatin-resistance-in-nasopharyngeal-carcinoma-by-repressing-jag1
#4
Lei Shi, Wei Yin, Zhiyu Zhang, Guanggang Shi
Therapy against nasopharyngeal carcinoma (NPC) is hurdled by chemoresistance. Recent studies found that microRNA (miRNA) are important regulators of cancer resistance. In this study, we aimed to explore the role and mechanism of miR-26b in regulating NPC cisplatin (CDDP) resistance. Real-time PCR was used to evaluate miR-26b levels in CDDP-resistant and CDDP-sensitive NPC cells, as well as human NPC tissues. MiR-26b was ectopically overexpressed in CDDP-resistant cells, followed by monitoring changes in cell viability and apoptosis...
December 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/28203047/transplantation-of-autologous-chondrocytes-ex-vivo-expanded-using-thermoreversible-gelation-polymer-in-a-rabbit-model-of-articular-cartilage-defect
#5
Sivaraman Arumugam, Balasubramanyan Bhupesh Karthik, Rajeswar Chinnuswami, Yuichi Mori, Hiroshi Yoshioka, Rajappa Senthilkumar, Rajmohan Mathaiyan, Karthick Ramalingam, Preethy Senthilkumar, Samuel J K Abraham
Graft failure due to de-differentiation of the chondrocytes during in vitro culture and after transplantation is a major hurdle in Autologous Chondrocyte Implantation (ACI). We, herein, report the transplantation of autologous chondrocytes ex vivo expanded using a Thermo-reversible Gelation Polymer (TGP) in a rabbit model. A full thickness chondral defect was created in one of the knee joints in each of the six rabbits of the study and autologous chondrocytes in vitro expanded using TGP scaffold were transplanted after 10 weeks...
June 2017: Journal of Orthopaedics
https://www.readbyqxmd.com/read/28199114/curcumin-loaded-blood-stable-polymeric-micelles-for-enhancing-therapeutic-effect-on-erythroleukemia
#6
Feirong Gong, Dan Chen, Xin Teng, Junhua Ge, Xianfeng Ning, Ya-Ling Shen, Jian Li, Shanfeng Wang
Curcumin has high potential in suppressing many types of cancer and overcoming multi-drug resistance in a multi-faceted manner by targeting diverse molecular targets. However, the rather low systemic bioavailability resulted from its extremely low aqueous solubility and rapid metabolism and excretion in vivo has hampered its application in cancer therapy. With the primary aim to increase the aqueous solubility of curcumin while retaining its stability in the blood circulation before it accesses the tumor site, here we report preparation of curcumin-loaded N-(tert-butoxycarbonyl)-L-phenylalanine end-capped methoxy-poly(ethylene glycol)-block-poly(ε-caprolactone) (mPEG-PCL-Phe(Boc)) micelles with high stability both in vitro and in vivo and anti-tumor efficacy...
February 15, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28197943/hydrothermal-synthesized-magnetically-separable-mesostructured-h2ti3o7-%C3%AE-fe2o3-nanocomposite-for-organic-dye-removal-via-adsorption-and-its-regeneration-reuse-through-synergistic-non-radiation-driven-h2o2-activation
#7
Harsha Narayani, Manu Jose, K Sriram, Satyajit Shukla
Hydrogen titanate (H2Ti3O7) nanotubes/nanosheets (HTN) are emerging class of adsorbent material which possess unique property of activating hydrogen peroxide (H2O2) to generate the reactive oxygen species (ROS), such as superoxide radical ions (O2(.-)) and hydroxyl radicals (·OH), effective in the decomposition of surface-adsorbed dye. However, HTN are non-magnetic which create hurdle in their effective separation from the treated aqueous solution. To overcome this issue, magnetic nanocomposites (HTNF) composed of HTN and maghemite (γ-Fe2O3) nanoparticles have been processed by subjecting the core-shell magnetic photocatalyst consisting of γ-Fe2O3/silica (SiO2)/titania (TiO2), having varying amounts of TiO2 in the shell to the hydrothermal conditions...
February 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28196091/pre-breeding-of-lentil-lens-culinaris-medik-for-herbicide-resistance-through-seed-mutagenesis
#8
Muhammad Rizwan, Muhammad Aslam, Muhammad Jawad Asghar, Ghulam Abbas, Tariq Mahmud Shah, Hussein Shimelis
Lentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116) were treated with two different concentrations of ethyl methanesulfonate (EMS; 0.1 and 0.2%), hydrazine hydrate (HH; 0.02 and 0.03%) and sodium azide (SA; 0.01 and 0.02%) to develop M1 seed. The M2 was screened against two herbicides including Ally Max 28...
2017: PloS One
https://www.readbyqxmd.com/read/28193218/a-combined-model-reduction-algorithm-for-controlled-biochemical-systems
#9
Thomas J Snowden, Piet H van der Graaf, Marcus J Tindall
BACKGROUND: Systems Biology continues to produce increasingly large models of complex biochemical reaction networks. In applications requiring, for example, parameter estimation, the use of agent-based modelling approaches, or real-time simulation, this growing model complexity can present a significant hurdle. Often, however, not all portions of a model are of equal interest in a given setting. In such situations methods of model reduction offer one possible approach for addressing the issue of complexity by seeking to eliminate those portions of a pathway that can be shown to have the least effect upon the properties of interest...
February 13, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/28192137/a-strategic-approach-for-direct-recovery-and-stabilization-of-fusarium-sp-ict-sac1-cutinase-from-solid-state-fermented-broth-by-carrier-free-cross-linked-enzyme-aggregates
#10
Sandeep A Chaudhari, Rekha S Singhal
The major hurdles in commercial exploitation of cutinase (having both esterolutic and lipolytic activities) with potent industrial applications are its high production cost, operational instability and reusability. Although commercially available in immobilized form, its immobilization process (synthesis of support/carrier) makes it expensive. Herein we tried to address multiple issues of production cost, stability, and reusability, associated with cutinase. Waste watermelon rinds, an agroindustrial waste was considered as a cheap support for solid state fermentation (SSF) for cutinase production by newly isolated Fusarium sp...
February 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28188786/increased-glycosylation-efficiency-of-recombinant-proteins-in-escherichia-coli-by-auto-induction
#11
Ning Ding, Chunguang Yang, Shenxia Sun, Lichi Han, Yao Ruan, Longhua Guo, Xuejun Hu, Jianing Zhang
Escherichia coli cells have been considered as promising hosts for producing N-glycosylated proteins since the successful production of N-glycosylated protein in E. coli with the pgl (N-linked protein glycosylation) locus from Campylobacter jejuni. However, one hurdle in producing N-glycosylated proteins in large scale using E. coli is inefficient glycan glycosylation. In this study, we developed a strategy for the production of N-glycosylated proteins with high efficiency via an optimized auto-induction method...
February 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28188529/dual-color-multiplex-analysis-of-protein-microarrays-for-precision-medicine
#12
Solomon Yeon, Florian Bell, Michael Shultz, Grace Lawrence, Michael Harpole, Virginia Espina
Generating molecular information in a clinically relevant time frame is the first hurdle to truly integrating precision medicine in health care. Reverse phase protein microarrays are being utilized in clinical trials for quantifying posttranslationally modified signal transduction proteins and cellular signaling pathways, allowing direct comparison of the activation state of proteins from multiple specimens, or individual patient specimens, within the same array. This technology provides diagnostic and therapeutic information critical to precision medicine...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28187410/jllumina-a-comprehensive-java-based-api-for-statistical-illumina-infinium-humanmethylation450-and-methylationepic-data-processing
#13
Diogo Almeida, Ida Skov, Jesper Lund, Afsaneh Mohammadnejad, Artur Silva, Fabio Vandin, Qihua Tan, Jan Baumbach, Richard Röttger
Measuring differential methylation of the DNA is the nowadays most common approach to linking epigenetic modifications to diseases (called epigenome-wide association studies, EWAS). For its low cost, its efficiency and easy handling, the Illumina HumanMethylation450 BeadChip and its successor, the Infinium MethylationEPIC BeadChip, is the by far most popular techniques for conduction EWAS in large patient cohorts. Despite the popularity of this chip technology, raw data processing and statistical analysis of the array data remains far from trivial and still lacks dedicated software libraries enabling high quality and statistically sound downstream analyses...
December 18, 2016: Journal of Integrative Bioinformatics
https://www.readbyqxmd.com/read/28187294/comprehensible-knowledge-model-creation-for-cancer-treatment-decision-making
#14
Muhammad Afzal, Maqbool Hussain, Wajahat Ali Khan, Taqdir Ali, Sungyoung Lee, Eui-Nam Huh, Hafiz Farooq Ahmad, Arif Jamshed, Hassan Iqbal, Muhammad Irfan, Manzar Abbas Hydari
BACKGROUND: A wealth of clinical data exists in clinical documents in the form of electronic health records (EHRs). This data can be used for developing knowledge-based recommendation systems that can assist clinicians in clinical decision making and education. One of the big hurdles in developing such systems is the lack of automated mechanisms for knowledge acquisition to enable and educate clinicians in informed decision making. MATERIALS AND METHODS: An automated knowledge acquisition methodology with a comprehensible knowledge model for cancer treatment (CKM-CT) is proposed...
January 29, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28186887/accelerated-singular-value-based-ultrasound-blood-flow-clutter-filtering-with-randomized-singular-value-decomposition-rsvd-and-randomized-spatial-downsampling
#15
Pengfei Song, Joshua Trzasko, Armando Manduca, Bo Qiang, Ramanathan Kadirvel, David Kallmes, Shigao Chen
Singular value decomposition (SVD)-based ultrasound blood flow clutter filters have recently demonstrated substantial improvement in clutter rejection for ultrafast plane wave microvessel imaging, and have become the commonly used clutter filtering method for many novel ultrafast imaging applications such as functional ultrasound and super-resolution imaging. At present, however, the computational burden of SVD remains as a major hurdle for practical implementation and clinical translation of this method. To address this challenge, in the study we present two blood flow clutter filtering methods based on randomized singular value decomposition (rSVD) and randomized spatial downsampling to accelerate SVD clutter filtering with minimal compromise to the clutter filter performance...
February 7, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28185995/study-protocol-community-links-to-establish-alcohol-recovery-clear-for-women-leaving-jail
#16
Jennifer E Johnson, Yael Chatav Schonbrun, Bradley Anderson, Megan Kurth, Christine Timko, Michael Stein
PURPOSE: This article describes the protocol for a randomized effectiveness trial of a method to link alcohol use disordered women who are in pretrial jail detention with post-release 12-step mutual help groups. BACKGROUND: Jails serve 15 times more people per year than do prisons and have very short stays, posing few opportunities for treatment or treatment planning. Alcohol use is associated with poor post-jail psychosocial and health outcomes including sexually transmitted diseases and HIV, especially for women...
February 7, 2017: Contemporary Clinical Trials
https://www.readbyqxmd.com/read/28185836/differential-gene-expression-profiling-of-human-adipose-stem-cells-differentiating-into-smooth-muscle-like-cells-by-tgf%C3%AE-1-bmp4
#17
Ayşe Eser Elçin, Mahmut Parmaksiz, Arin Dogan, Sukran Seker, Serap Durkut, Klara Dalva, Yaşar Murat Elçin
Regenerative repair of the vascular system is challenging from the perspectives of translational medicine and tissue engineering. There are fundamental hurdles in front of creating bioartificial arteries, which involve recaputilation of the three-layered structure under laboratory settings. Obtaining and maintaining smooth muscle characteristics is an important limitation, as the transdifferentiated cells fail to display mature phenotype. This study aims to shed light on the smooth muscle differentiation of human adipose stem cells (hASCs)...
February 6, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28185769/intact-wound-repair-activity-of-human-mesenchymal-stem-cells-after-ym155-mediated-selective-ablation-of-undifferentiated-human-embryonic-stem-cells
#18
Keun-Tae Kim, Ho-Chang Jeong, C-Yoon Kim, Eun-Young Kim, Si-Hyun Heo, Seung-Ju Cho, Ki-Sung Hong, Hyuk-Jin Cha
BACKGROUND: Risk of teratoma formation during human pluripotent stem cell (hPSC)-based cell therapy is one of the technical hurdles that must be resolved before their wider clinical application. To this end, selective ablation of undifferentiated hPSCs has been achieved using small molecules whose application should be safe for differentiated cells derived from the hPSCs. OBJECTIVE: However, the functional safety of such small molecules in the cells differentiated from hPSCs has not yet been extensively validated...
January 31, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28185742/do-we-need-a-vaccine-against-moraxella-catarrhalis-in-chronic-lung-disease-what-are-the-options-and-opportunities
#19
REVIEW
Antonia C Perez, Timothy F Murphy
Moraxella catarrhalis is the second most common cause of exacerbations in adults with COPD, resulting in enormous morbidity and mortality in this clinical setting. Vaccine development for M. catarrhalis has lagged behind the other two important causes of exacerbations in COPD, nontypeable Haemophilus influenzae and Streptococcus pneumoniae. While no licensed vaccine is currently available for M. catarrhalis, several promising candidate vaccine antigens have been identified and characterized and are close to entering clinical trials...
February 6, 2017: Vaccine
https://www.readbyqxmd.com/read/28185539/prospects-of-gene-therapy-for-pulmonary-diseases-progress-and-limitations
#20
Garyfalia Karponi, Nikolaos Zogas, Kalliopi Domvri, Paul Zarogoulidis, Georgia Trakada, Stefanos Roumeliotis, Wolfgang Hohenforst-Schmidt, Kaid Darwiche, Lutz Freitag, Konstantinos Zarogoulidis
BACKGROUND: Despite the proof of principle that gene therapy can cure various monogenic diseases, limited clinical progress has been noted for gene therapy of the respiratory system. Certain anatomic features of the lungs, along with the suboptimal gene delivery vehicles utilized up to now, have significantly delayed successful clinical practice. Thus, the need for additional improvements towards safety and efficacy of the procedure is indispensable. OBJECTIVE: The objective of this work was to review the progress and limitations of gene therapy in the treatment of lung disease with a focus on monogenic disease, chronic obstructive pulmonary disease and asthma and to present studies that provide a proof of principle that it works in different model systems and in patients...
February 9, 2017: Medicinal Chemistry
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