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https://www.readbyqxmd.com/read/26378228/identification-of-the-iswi-chromatin-remodeling-complex-of-the-early-branching-eukaryote-trypanosoma-brucei
#1
Tara M Stanne, Mani Shankar Narayanan, Sophie Ridewood, Alexandra Ling, Kathrin Witmer, Manish Kushwaha, Simone Wiesler, Bill Wickstead, Jennifer Wood, Gloria Rudenko
ISWI chromatin remodelers are highly conserved in eukaryotes and are important for the assembly and spacing of nucleosomes, thereby controlling transcription initiation and elongation. ISWI is typically associated with different subunits, forming specialized complexes with discrete functions. In the unicellular parasite Trypanosoma brucei, which causes African sleeping sickness, TbISWI down-regulates RNA polymerase I (Pol I)-transcribed variant surface glycoprotein (VSG) gene expression sites (ESs), which are monoallelically expressed...
November 6, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26172005/influence-of-respiratory-viruses-on-the-evaluation-of-the-13-valent-pneumococcal-conjugate-vaccine-effectiveness-in-children-under-5-years-old-a-time-series-study-for-the-2001-2013-period
#2
Ángela Gentile, María del Valle Juarez, María Florencia Luciön, Viviana Sandra Romanin, Norberto Giglio, Julia Bakin
INTRODUCTION: S treptococcus pneumoniae is the main agent in bacterial consolidated pneumonias. In 2012, the 13-valent pneumococcal conjugate vaccine was introduced in the Argentine national immunization schedule for immunocompetent children as of two months old with a two-dose schedule plus a booster. OBJECTIVE: To analyze the influence of respiratory viruses on the evaluation of the 13-valent pneumococcal conjugate vaccine effectiveness in relation to the number of hospitalizations for radiologically-confirmed consolidated pneumonias (RCCP)...
August 2015: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/25907133/binding-of-imidazole-1-methylimidazole-and-4-nitroimidazole-to-yeast-cytochrome-c-peroxidase-ccp-and-the-distal-histidine-mutant-ccp-h52l
#3
James E Erman, Diana Chinchilla, Jason Studer, Lidia B Vitello
Imidazole, 1-methylimidazole and 4-nitroimidazole bind to yeast cytochrome c peroxidase (yCcP) with apparent equilibrium dissociation constants (KD(app)) of 3.3±0.4, 0.85±0.11, and ~0.2M, respectively, at pH7. This is the weakest imidazole binding to a heme protein reported to date and it is about 120 times weaker than imidazole binding to metmyoglobin. Spectroscopic changes associated with imidazole and 1-methylimidazole binding to yCcP suggest partial ionization of bound imidazole to imidazolate. The pKa for ionization of bound imidazole is estimated to be 7...
August 2015: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/22940664/-mitral-valve-replacement-after-previous-coronary-artrey-bypass-grafting-cabg-with-functioning-left-internal-thoracic-artery-lita-grafts-in-an-elderly-patient-report-of-a-case
#4
Hiroshi Furukawa, Hitoshi Aono, Masanobu Samukawa, Akihiko Ohkado
An 85-year-old woman had a history of coronary artery bypass grafting (CABG) performed 7 years ago, and dyspnea on effort had been worsening recently. Since echocardiography showed severe mitral valve regurgitation( MR), mitral valve repair was suggested. Preoperative enhanced computed tomography (CT) showed the patent functioning left internal thoracic artery (LITA) graft. Mitral valve replacement (MVR) using a 25 mm CEP bioprosthesis was performed successfully via resternotomy without any intraoperative injury of the heart...
September 2012: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/21031514/comparison-of-magnetic-resonance-imaging-with-transthoracic-echocardiography-in-the-diagnosis-of-ventricular-septal-defect-associated-coronary-cusp-prolapse
#5
COMPARATIVE STUDY
Norihiko Yoshimura, Yoshiro Hori, Yousuke Horii, Hiroshi Suzuki, Satoshi Hasegawa, Masashi Takahashi, Hiroshi Watanabe
PURPOSE: To compare magnetic resonance imaging (MRI) with transthoracic echocardiography (TTE) in the diagnosis of coronary cusp prolapse (CCP) as a complication of ventricular septal defect (VSD). MATERIALS AND METHODS: Twenty-three patients (10 males, 13 females, mean age 9 years) with clinically suspected CCP were included in a retrospective study. Long and short axis cine images and electrocardiogram (ECG)-gated axial T1-weighted image MRI images were evaluated for deformity and protrusion of right and noncoronary cusps...
November 2010: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/19117496/outcomes-from-a-respiratory-coordinated-care-program-rccp-providing-community-based-interventions-for-copd-patients-from-1998-to-2006
#6
Nick Spiliopoulos, Judith Donoghue, Elizabeth Clark, Mary Dunford
The Respiratory Coordinated Care Program (RCCP) based at St George Hospital, is a specialised community program designed to assist people with advanced COPD (DRG groupings E65A/E65B) to live optimally well at home. The aim of the program is to reduce hospital admission rates, readmission rates, and hospital length of stay (LOS). Additional components of RCCP include a Pulmonary Rehabilitation program and an early discharge service. An improvement in patient outcomes over subsequent years 1998-2006, is demonstrated when compared to the national peer DRG with regards to LOS, readmission rates and hospital admissions per patient per year, pre and post recruitment to the RCCP...
December 2008: Contemporary Nurse
https://www.readbyqxmd.com/read/17554094/management-of-high-caries-risk-and-high-caries-activity-patients-rampant-caries-control-program-rccp
#7
Sandra Guzmán-Armstrong, John J Warren
The purpose of this article is to describe the Rampant Caries Control Program established in the operative dentistry third-year clinic at the University of Iowa College of Dentistry. This program represents a new approach to the management of high caries risk and high caries activity patients who come to the school. The patients are referred initially to the third-year operative clinic only for disease control (phase I) to help them decrease the caries risk and activity. The disease control phase has three important components: 1) caries risk assessment evaluation and reevaluation throughout their treatment, in which individual risk factors are identified and recommendations are made; 2) caries removal and placement of transitional restorations using fluoride release restorative materials (glass ionomers); and 3) chemotherapeutic agents and preventive treatment in which a therapeutic regimen for prevention and nonsurgical treatment is established according to each patient's individual risk factors...
June 2007: Journal of Dental Education
https://www.readbyqxmd.com/read/17128976/characterization-of-four-covalently-linked-yeast-cytochrome-c-cytochrome-c-peroxidase-complexes-evidence-for-electrostatic-interaction-between-bound-cytochrome-c-molecules
#8
Siddhartha Nakani, Lidia B Vitello, James E Erman
Four covalent complexes between recombinant yeast cytochrome c and cytochrome c peroxidase (rCcP) were synthesized via disulfide bond formation using specifically designed protein mutants (Papa, H. S., and Poulos, T. L. (1995) Biochemistry 34, 6573-6580). One of the complexes, designated V5C/K79C, has cysteine residues replacing valine-5 in rCcP and lysine-79 in cytochrome c with disulfide bond formation between these residues linking the two proteins. The V5C/K79C complex has the covalently bound cytochrome c located on the back-side of cytochrome c peroxidase, approximately 180 degrees from the primary cytochrome c-binding site as defined by the crystallographic structure of the 1:1 noncovalent complex (Pelletier, H...
December 5, 2006: Biochemistry
https://www.readbyqxmd.com/read/16893189/characterization-of-a-covalently-linked-yeast-cytochrome-c-cytochrome-c-peroxidase-complex-evidence-for-a-single-catalytically-active-cytochrome-c-binding-site-on-cytochrome-c-peroxidase
#9
Siddartha Nakani, Thanarat Viriyakul, Robert Mitchell, Lidia B Vitello, James E Erman
A covalent complex between recombinant yeast iso-1-cytochrome c and recombinant yeast cytochrome c peroxidase (rCcP), in which the crystallographically defined cytochrome c binding site [Pelletier, H., and Kraut, J. (1992) Science 258, 1748-1755] is blocked, was synthesized via disulfide bond formation using specifically engineered cysteine residues in both yeast iso-1-cytochrome c and yeast cytochrome c peroxidase [Papa, H. S., and Poulos, T. L. (1995) Biochemistry 34, 6573-6580]. Previous studies on similar covalent complexes, those that block the Pelletier-Kraut crystallographic site, have demonstrated that samples of the covalent complexes have detectable activities that are significantly lower than those of wild-type yCcP, usually in the range of approximately 1-7% of that of the wild-type enzyme...
August 15, 2006: Biochemistry
https://www.readbyqxmd.com/read/16584174/heterologous-overexpression-and-purification-of-cytochrome-c-from-rhodobacter-capsulatus-and-a-mutant-k42e-in-the-dimerization-region-mutation-does-not-alter-oligomerization-but-impacts-the-heme-iron-spin-state-and-nitric-oxide-binding-properties
#10
Wilhelmina M Huston, Colin R Andrew, Amy E Servid, Alison L McKay, Andrew P Leech, Clive S Butler, James W B Moir
Rhodobacter capsulatus cytochrome c' (RCCP) has been overexpressed in Escherichia coli, and its spectroscopic and ligand-binding properties have been investigated. It is concluded that the heterologously expressed protein is assembled correctly, as judged by UV-vis absorption, EPR, and resonance Raman (RR) spectroscopy of the unligated protein as well as forms in which the heme is ligated by CO or NO. To probe the oligomerization state of RCCP and its potential influence on heme reactivity, we have compared the properties of wild-type RCCP with a mutant (K42E) that lacks a salt bridge at the subunit interface...
April 11, 2006: Biochemistry
https://www.readbyqxmd.com/read/15952773/accessibility-of-the-distal-heme-face-rather-than-fe-his-bond-strength-determines-the-heme-nitrosyl-coordination-number-of-cytochromes-c-evidence-from-spectroscopic-studies
#11
Colin R Andrew, Lenord J Kemper, Tammy L Busche, Arianne M Tiwari, Michael C Kecskes, James M Stafford, Lea C Croft, Shen Lu, Pierre Moënne-Loccoz, Willa Huston, James W B Moir, Robert R Eady
The heme coordination chemistry and spectroscopic properties of Rhodobacter capsulatus cytochrome c' (RCCP) have been compared to data from Alcaligenes xylosoxidans (AXCP), with the aim of understanding the basis for their different reactivities with nitric oxide (NO). Whereas ferrous AXCP reacts with NO to form a predominantly five-coordinate heme-nitrosyl complex via a six-coordinate intermediate, RCCP forms an equilibrium mixture of six-coordinate and five-coordinate heme-nitrosyl species in approximately equal proportions...
June 21, 2005: Biochemistry
https://www.readbyqxmd.com/read/15299853/structure-of-cytochrome-c-from-rhodobacter-capsulatus-strain-st-louis-an-unusual-molecular-association-induced-by-bridging-zn-ions
#12
T H Tahirov, S Misaki, T E Meyer, M A Cusanovich, Y Higuchi, N Yasuoka
Rhodobacter capsulatus strain St Louis cytochrome c' (RCCP-SL) has been crystallized and the structure solved by molecular replacement. It was refined at 2.1 A resolution to an R value of 18.4%, and compared with Rhodobacter capsulatus strain M110 cytochrome c' (RCCP-M110). Although these two proteins are very similar in sequence and structure, the intermolecular interaction is largely different. In RCCP-M110, the molecules dimerize through interaction of helix B to form an antiparallel arrangement. When crystallized in the presence of Zn ions, molecules of RCCP-SL were found to be arranged as linear polymers connected by the bridging Zn ions...
November 1, 1997: Acta Crystallographica. Section D, Biological Crystallography
https://www.readbyqxmd.com/read/14745149/severity-indices-of-right-coronary-cusp-prolapse-and-aortic-regurgitation-complicating-ventricular-septal-defect-in-the-outlet-septum-which-defect-should-be-closed
#13
Hideshi Tomita, Yoshio Arakaki, Yasuo Ono, Osamu Yamada, Toshikatsu Yagihara, Shigeyuki Echigo
BACKGROUND: The factors that may determine the evolution of right coronary cusp prolapse (RCCP) and regurgitation (AR) associated with a ventricular septal defect in the outlet septum (outlet VSD) have not been clarified. METHODS AND RESULTS: The Doppler echocardiograms of 316 patients were grouped according to both the development of RCCP, and the values of the right coronary cusp deformity index (RCCD) and the right coronary cusp imbalance index (R/L). All detected AR was </= slight, and not progressive in patients with both RCCD <0...
February 2004: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/12962502/temperature-ph-and-solvent-isotope-dependent-properties-of-the-active-sites-of-resting-state-and-cyanide-ligated-recombinant-cytochrome-c-peroxidase-h52l-revealed-by-proton-hyperfine-resonance-spectra
#14
James D Satterlee, Marina I Savenkova, Miriam Foshay, James E Erman
Comparative proton NMR studies have been carried out on high-spin and low-spin forms of recombinant native cytochrome c peroxidase (rCcP) and its His52 --> Leu variant. Proton NMR spectra of rCcP(H52L) (high spin) and rCcP(H52L)CN (low spin) reveal the presence of multiple enzyme forms in solution, whereas only single enzyme forms are found in spectra of wild-type and recombinant wild-type CcP and CcPCN near neutral pH. The spectroscopic behaviors of these forms have been studied in detail when pH, temperature, and solvent isotope composition were varied...
September 16, 2003: Biochemistry
https://www.readbyqxmd.com/read/12827249/the-development-and-evaluation-of-consensus-chloroplast-primer-pairs-that-possess-highly-variable-sequence-regions-in-a-diverse-array-of-plant-taxa
#15
Sang-Min Chung, Jack E Staub
Although universal or consensus chloroplast primers are available, they are limited by their number and genomic distribution. Therefore, a set of consensus chloroplast primer pairs for simple sequence repeats (ccSSRs) analysis was constructed from tobacco (Nicotiana tabacum L.) chloroplast sequences. These were then tested for their general utility in the genetic analysis of a diverse array of plant taxa. In order to increase the number of ccSSRs beyond that previously reported, the target sequences for SSR motifs was set at A or T ( n >/= 7) mononucleotide repeats...
August 2003: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/11407730/imbalance-of-cusp-width-and-aortic-regurgitation-associated-with-aortic-cusp-prolapse-in-ventricular-septal-defect
#16
H Tomita, Y Arakaki, Y Ono, O Yamada, T Yagihara, S Echigo
The Doppler echocardiograms of the aortic valve and associated aortic regurgitation (AR) were reviewed in 72 patients with a ventricular septal defect (VSD). Group I comprised 13 patients without any deformity of the aortic cusp for > or = 10 years, group 2 included 35 patients who did not develop AR for > or = 10 years after right coronary cusp prolapse (RCCP) was first detected, group 3 comprised 11 patients with RCCP and AR in whom the AR remained subclinical for > or = 10 years, and group 4 was 13 patients who underwent surgical treatment because of moderate to severe AR...
June 2001: Japanese Circulation Journal
https://www.readbyqxmd.com/read/10346559/practice-profile-riverside-critical-care-physicians-rccp-columbus-ohio
#17
(no author information available yet)
No abstract text is available yet for this article.
1998: Hospitalist
https://www.readbyqxmd.com/read/9826513/basis-for-monomer-stabilization-in-rhodopseudomonas-palustris-cytochrome-c-derived-from-the-crystal-structure
#18
N Shibata, S Iba, S Misaki, T E Meyer, R G Bartsch, M A Cusanovich, Y Morimoto, Y Higuchi, N Yasuoka
The crystal structure of an unusual monomeric cytochrome c' from Rhodopseudomonas palustris (RPCP) has been determined at 2.3 A resolution. RPCP has the four-helix (helices A, B, C and D) bundle structure similar to dimeric cytochromes c'. However the amino acid composition of the surface of helices A and B in RPCP is remarkably different from that of the dimeric cytochromes c'. This surface forms the dimer interface in the latter proteins. RPCP has seven charged residues on this surface contrary to the dimeric cytochromes c', which have only two or three charged groups on the corresponding surface...
December 4, 1998: Journal of Molecular Biology
https://www.readbyqxmd.com/read/9637840/-outcome-of-emergency-surgery-for-stanford-type-a-aneurysms-selection-of-operative-procedures-and-supported-systems-of-cerebral-protection
#19
T Ichihara, H Ishida, T Asakura, Y Sakai, K Yasuura
Fourteen surgical cases of acute dissecting aneurysms of Stanford type A were reported. Clinical symptoms, operative methods, pathophysiology, cerebral protection, supported systems, and prognosis were examined. All patients underwent surgery within 5 days of symptom onset. The patient population consisted 8 males and 6 females with a mean age of 56.6 years. Ascending aortic replacement was performed using a vascular graft 12 patients. ECC was performed under deep hypothermia. Femoral artery cannulation and retrograde cerebral perfusions was performed in all cases...
June 1998: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/9635221/resolving-conflict-creatively-evaluating-the-developmental-effects-of-a-school-based-violence-prevention-program-in-neighborhood-and-classroom-context
#20
J L Aber, S M Jones, J L Brown, N Chaudry, F Samples
This study evaluated the short-term impact of a school-based violence prevention initiative on developmental processes thought to place children at risk for future aggression and violence and examined the influence of classroom and neighborhood contexts on the effectiveness of the violence prevention initiative. Two waves of developmental data (fall and spring) were analyzed from the 1st year of the evaluation of the Resolving Conflict Creatively Program (RCCP), which includes 5053 children from grades two to six from 11 elementary schools in New York City...
1998: Development and Psychopathology
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