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Michael A Taylor, Jong W Yu, Thomas L Howell, Stephen H Jones
Vibrio parahaemolyticus is the leading cause of seafood-borne human infections in the United States, and many of these illnesses are associated with consumption of raw molluscan shellfish. V. parahaemolyticus levels in shellfish vary temporally and spatially with environmental conditions in and around production areas. The objective of this study was to study the potential for reducing levels of V. parahaemolyticus in live oysters by relaying them during higher-risk warm weather to a site with elevated salinity and consistently low V...
March 15, 2018: Journal of Food Protection
Thi Thu Tra Vu, Thomas Alter, Stephan Huehn
This study was conducted to determine the prevalence of Vibrio spp. in retail seafood in Berlin, Germany. A total of 160 raw seafood samples from supermarkets and seafood shops, consisting of shrimp ( n = 80) and bivalves ( n = 80), were investigated for the presence of Vibrio spp. using the International Organization for Standardization ISO/TS 21872 method and a multiplex PCR. The overall prevalence of Vibrio spp. in retail seafood was 55% (95% CI: 47.2 to 62.8%). The prevalence of Vibrio spp. in shrimp was slightly higher than in bivalves (57...
March 8, 2018: Journal of Food Protection
Chung Thong Lim, Márta Korbonits
Pituitary adenomas are the third most common central nervous system tumours and arise from the anterior pituitary within the pituitary fossa. The signs and symptoms of patients with pituitary adenomas vary from 'mass effects' caused by a large adenoma to features secondary to excess pituitary hormones produced by the functioning pituitary adenoma. Detailed histopathological assessment, based on novel classifications and the latest WHO guidelines, helps to categorise pituitary adenomas into different subtypes and identify features that, in some cases, help to predict their behaviour...
March 2, 2018: Endocrine Practice
Arleta K Skrzynska, Elisabetta Maiorano, Marco Bastaroli, Fatemeh Naderi, Jesús M Míguez, Gonzalo Martínez-Rodríguez, Juan M Mancera, Juan A Martos-Sitcha
The hypothalamus-pituitary-interrenal (HPI) and hypothalamus-sympathetic-chromaffin cell (HSC) axes are involved in the regulation of the stress response in teleost. In this regard, the activation of a complex network of endocrine players is needed, including corticotrophin-releasing hormone (Crh), Crh binding protein (Crhbp), proopiomelanocortin (Pomc), thyrotropin-releasing hormone (Trh), arginine vasotocin (Avt), and isotocin (It) to finally produce pleiotropic functions. We aimed to investigate, using the gilthead sea bream ( Sparus aurata ) as a biological model, the transcriptomic response of different endocrine factors ( crh, crhbp, pomc s, trh ), neuropeptides ( avt and it ), and their specific receptors ( avtrv1a, avtrv2 , and itr ) in four important target tissues (hypothalamus, pituitary, kidney and liver), after an acute stress situation...
2018: Frontiers in Physiology
Daniel Castillo, Diliana Pérez-Reytor, Nicolás Plaza, Sebastián Ramírez-Araya, Carlos J Blondel, Gino Corsini, Roberto Bastías, David E Loyola, Víctor Jaña, Leonardo Pavez, Katherine García
Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. As reported in other countries, after the rise and fall of the pandemic strain in Chile, other post-pandemic strains have been associated with clinical cases, including strains lacking the major toxins TDH and TRH. Since the presence or absence of tdh and trh genes has been used for diagnostic purposes and as a proxy of the virulence of V. parahaemolyticus isolates, the understanding of virulence in V. parahaemolyticus strains lacking toxins is essential to detect these strains present in water and marine products to avoid possible food-borne infection...
2018: Frontiers in Microbiology
Christian Schmidt, Alper Öner, Miriam Mann, Katja Krockenberger, Melanie Abbondanzieri, Bernard Brandewiede, Armin Brüge, Gisela Hostenkamp, Axel Kaiser, Henriette Neumeyer, Andreas Ziegler
BACKGROUND: Cardiovascular diseases are the major cause of death globally and represent a major economic burden on health care systems. Positive effects of disease management programs have been shown for patients with heart failure (HF). Remote monitoring and telemonitoring with active intervention are beneficial in atrial fibrillation (AF) and therapy-resistant hypertension (TRH), respectively. For these patients, we have developed a novel integrated care concept (NICC) which combines telemedicine with intensive support by a care center, including a call center, an integrated care network including inpatient and outpatient care providers and guideline therapy for patients...
February 20, 2018: Trials
Umit Yilmaz, Suat Tekin, Mehmet Demir, Yilmaz Cigremis, Suleyman Sandal
The aim of this study was to evaluate the impact of intracerebroventricular chronic fibroblast growth factor 21 (FGF21) infusion on hypothalamic-pituitary-thyroid (HPT) axis, energy metabolism, food intake and body weight. Thirty male Wistar albino rats were used and divided into three groups including control, sham (vehicle) and FGF21 infused groups (n = 10). Intracerebroventricularly, FGF21 and vehicle groups were infused for 7 days with FGF21 (0.72 µg/day) and artificial cerebrospinal fluid, respectively...
February 7, 2018: Journal of Physiological Sciences: JPS
Xin-Yuan Cao, Xu Hua, Jian-Wei Xiong, Wen-Ting Zhu, Jun Zhang, Ling Chen
Triclosan (TCS), a broad-spectrum antimicrobial agent, is widely used in clinical settings and various personal care products. The aim of this study was to evaluate the influence of TCS on reproductive endocrine and function. Here, we show that the exposure of adult female mice to 10 or 100 mg/kg/day TCS caused prolongation of diestrus, and decreases in antral follicles and corpora lutea within 2 weeks. TCS mice showed decreases in the levels of serum luteinizing hormone (LH), follicle-stimulating hormone (FSH) and progesterone, and gonadotrophin-releasing hormone (GnRH) mRNA with the lack of LH surge and elevation of prolactin (PRL)...
2018: Frontiers in Molecular Neuroscience
Christian Ott, Klaas F Franzen, Tobias Graf, Joachim Weil, Roland E Schmieder, Michael Reppel, Kai Mortensen
Ambulatory blood pressure (BP) and central BP are better predictors for overall cardiovascular risk and mortality than brachial BP. Renal denervation (RDN) has been shown to reduce office brachial and central BP as well as brachial ambulatory BP, but data on central ambulatory BP are limited. Patients (N = 94) with treatment resistant hypertension (TRH) who underwent RDN were included. Ambulatory BP, including central pressures, hemodynamics, and arterial stiffness were measured at baseline and 3, 6, 12 months after RDN by an oscillometric device (MobiloGraph™ )...
January 25, 2018: Journal of Clinical Hypertension
Claudia Rivetti, Bruno Campos, Benjamín Piña, Demetrio Raldúa, Yasuhiko Kato, Hajime Watanabe, Carlos Barata
Tryptophan hydroxylase (TRH) is the rate limiting enzyme in the serotonin synthesis. CRISPR-Cas9 technology was used to generate seven indel TRH mutants in Daphnia magna. Mono-allelic indel TRH-/+ clones showed normal levels of serotonin, measured by both immunohistochemistry and mass spectrometry (LC-MS/MS), whereas bi-allelic indel TRH-/- clones showed no detectable levels of serotonin. Life history and behavioral responses of TRH-/- clones showed the anti-phenotype of those exposed to selective serotonin reuptake inhibitors (SSRI)...
January 24, 2018: Scientific Reports
Lina Holmqvist, Kristina Bengtsson Boström, Thomas Kahan, Linus Schiöler, Miriam Qvarnström, Björn Wettermark, Per Hjerpe, Jan Hasselström, Karin Manhem
PURPOSE: To assess drug adherence in patients treated with ≥3 antihypertensive drug classes, with both controlled and uncontrolled blood pressure and describe associated factors for nonadherence. METHODS: Patients with hypertension, without cardiovascular comorbidity, aged >30 years treated with ≥3 antihypertensive drug classes were followed for 2 years. Both patients with treatment resistant hypertension (TRH) and patients with controlled hypertension were included...
January 19, 2018: Pharmacoepidemiology and Drug Safety
Rui Huang, Xiwen Liao, Qiaochuan Li
Background: Tumor protein p53 (TP53) mutations are not only a risk factor in acute myeloid leukemia (AML) but also a potential biomarker for individualized treatment options. This study aimed to investigate potential pathways and genes associated with TP53 mutations in adult de novo AML. Methods: An RNA sequencing dataset of adult de novo AML was downloaded from The Cancer Genome Atlas database. Differentially expressed genes (DEGs) were identified by edgeR of the R platform...
2018: OncoTargets and Therapy
Marina O Fernandez, Nadia S Bourguignon, Paula Arocena, Matías Rosa, Carlos Libertun, Victoria Lux-Lantos
Bisphenol A (BPA) is a component of polycarbonate plastics, epoxy resins and polystyrene found in many common products. Several reports revealed potent in vivo and in vitro effects. In this study we analyzed the effects of the exposure to BPA in the hypothalamic-pituitary-thyroid axis in female rats, both in vivo and in vitro. Female Sprague-Dawley rats were injected sc from postnatal day 1 (PND1) to PND10 with BPA: 500 μg 50 μl-1 oil (B500), or 50 μg 50 μl-1 (B50), or 5 μg 50 μl-1 (B5). Controls were injected with 50 μl vehicle during the same period...
January 2, 2018: Toxicology Letters
Kaoru Kobayashi, Yoshikazu Abe, Hiroshi Harada, Emiko Oota, Takuro Endo, Hiroo Takeda
1. The non-clinical pharmacokinetic profiles of rovatirelin, a novel thyrotropin-releasing hormone (TRH) analogue, were investigated in vivo and in vitro. 2. Rovatirelin orally administered to rats and dogs was rapidly absorbed and bioavailability was estimated to be 7.3 and 41.3%, respectively. The extent of plasma protein binding of rovatirelin in rats, dogs, and humans was low in all species (∼15%). The permeability of rovatirelin from blood to brain (permeability-surface area) ranged from 1.04 ± 0.14 to 1...
January 31, 2018: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Ignacio Ruiz-Jarabo, J A Martos-Sitcha, C Barragán-Méndez, G Martínez-Rodríguez, J M Mancera, F J Arjona
In euryhaline teleosts, the hypothalamus-pituitary-thyroid and hypothalamus-pituitary-interrenal axes (HPT and HPI, respectively) are regulated in response to environmental stimuli such as salinity changes. However, the molecular players participating in this physiological process in the gilthead seabream (Sparus aurata), a species of high value for aquaculture, are still not identified and/or fully characterized in terms of gene expression regulation. In this sense, this study identifies and isolates the thyrotropin-releasing hormone (trh) mRNA sequence from S...
December 23, 2017: Fish Physiology and Biochemistry
Di Sun, Liting Zhou, Shuyue Wang, Te Liu, Jian Zhu, Yiyang Jia, Jin Xu, Huaiji Chen, Qi Wang, Feng Xu, Yuezhu Zhang, Lin Ye
Di-(2-ethylhexyl) phthalate (DEHP) is extensively used in many personal care and consumer products, which has resulted in widespread human exposure. Limited studies have suggested that exposure to DEHP may affect thyroid function, but little is known about the effect and mechanisms of DEHP exposure on the hypothalamic-pituitary-thyroid axis (HPTA). The present study was conducted to elucidate the potential mechanisms in which DEHP disrupts the function of the HPTA. Wistar rats were administered DEHP by gavage at 0, 5, 50, and 500 mg/kg/day for 28 days and then sacrificed within 24 h following the last dose...
December 9, 2017: Endocrine Journal
S I Jacob, R J Geor, P S D Weber, P A Harris, M E McCue
Diagnosis of equine pituitary pars intermedia dysfunction (PPID) remains a challenge as multiple factors (stress, exercise, and time of year) influence ACTH and cortisol concentrations. To assess endocrine status in a study designed to evaluate the effects of age and diet on glucose and insulin dynamics, we performed thyrotropin-releasing hormone (TRH) stimulation tests and overnight dexamethasone suppression tests in March, May, August, and October on 16 healthy Thoroughbred and Standardbred mares and geldings...
April 2018: Domestic Animal Endocrinology
Ayako Sakuda, Chiho Suzuki-Minakuchi, Kazuhiro Matsui, Yurika Takahashi, Kazunori Okada, Hisakazu Yamane, Masaki Shintani, Hideaki Nojiri
The incompatibility (Inc) P-7 group plasmid pCAR1 can be efficiently transferred among bacteria in artificial microcosms in the presence of divalent cations Ca2+ and Mg2+ . One-on-one mating assays between Pseudomonas strains with different plasmids showed that the promotion of conjugation efficiency by divalent cations was exhibited in other plasmids, including pB10 and NAH7; however, this effect was larger in IncP-7 plasmids. The impact on pCAR1 conjugation differed according to donor-recipient pairs, and conjugation efficiency promotion was clearly detected between the donors P...
January 2018: Microbiology
Mohammad M Obaidat, Alaa E Bani Salman, Amira A Roess
Vibrio parahaemolyticus is a leading cause of seafood-associated illness. This study investigated the prevalence, virulence, and antibiotic resistance of V. parahaemolyticus in three low- and middle-income countries. Freshly caught fish samples (n = 330) imported to Jordan from Yemen, India, and Egypt were tested. The overall prevalence of V. parahaemolyticus was 15% (95% confidence interval: 11 to 19%). Three isolates (6%) were positive for the thermostable direct hemolysin-related (trh) gene, and all isolates was negative for the thermostable direct hemolysin (tdh) gene...
December 2017: Journal of Food Protection
Sara Elmahdi, Salina Parveen, Sylvia Ossai, Ligia V DaSilva, Michael Jahncke, John Bowers, John Jacobs
Vibrio parahaemolyticus (Vp) and Vibrio vulnificus (Vv) are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the USA. Though multiple-antibiotic resistant Vp and Vv have been reported resistance patterns in vibrios are not as well documented as other food-borne bacterial pathogens. Salinity relaying (SR) is a Post-Harvest Processing (PHP) treatment to reduce the abundance of these pathogens in shellfish harvested during the warmer months. The purpose of this study was to evaluate the antimicrobial susceptibility (AMS), pathogenicity and genetic profiles of Vp and Vv recovered from oysters during an oyster relay study...
November 17, 2017: Applied and Environmental Microbiology
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