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Lacey Lemonakis, KaWang Li, Jeremy M Adler, Cangliang Shen
This study aims to evaluate the microbiological quality and efficacy of antimicrobials to inactivate unstressed or cold-stress adapted Salmonella and Enterococcus on broiler carcasses and wings processed at a small USDA-inspected slaughter facility in West Virginia. The first part of the study included 42 carcasses that were pre- and secondarily-enriched in bacterial media followed by streak-plating onto XLT-4 and HardyCHROM™-agar Salmonella and confirmation using an API20E-kit. The aerobic plate counts (APC), Escherichia coli (ECC), total coliforms (TCC), and yeast/molds were analyzed on petri-films...
September 1, 2017: Poultry Science
Jun Xiang Ong, Victoria Yu Ting Pang, Li Min Tng, Wee Han Ang
A new preassembled ratiometric sensing platform was constructed from a coumarin donor and a rhodamine acceptor designed for through-bond energy transfer (TBET). A phenylacetylene linker was installed to disrupt the planarity of the extended conjugated system but retaining the efficient energy transfer between the donor and acceptor motifs. To demonstrate its versatility as a sensing platform, we conjugated recognition motifs through amide coupling reactions to yield two TBET chemosensors capable of sensing either endogenously produced NO and ClO-...
October 19, 2017: Chemistry: a European Journal
Murali Mohan Thota, Kakollu Sudha, D L Malini, Singiri Bindhu Madhavi
AIM: The aim of the study was to evaluate the effect of different irrigating solutions used in final irrigation on depth of sealer penetration into dentinal tubules. MATERIALS AND METHODS: Thirty recently extracted, human mandibular premolar teeth with single canals were randomly divided into two groups, and one of the two irrigants was used in each group - Group A (Chitosan) and Group B (Ethylenediaminetetraacetic acid). All the teeth were obturated with gutta-percha and AH 26(®) sealer labeled with fluorescent dye...
July 2017: Contemporary Clinical Dentistry
Tanoy Dutta, Falguni Chandra, Apurba L Koner
A "naked-eye" detection of health hazardous bisulfite (HSO3(-)) and hypochlorite (ClO(-)) using an indicator dye (Quinaldine Red, QR) in a wide range of pH is demonstrated. The molecule contains a quinoline moiety linked to an N,N-dimethylaniline moiety with a conjugated double bond. Treatment of QR with HSO3(-) and ClO(-), in aqueous solution at near-neutral pH, resulted in a colorless product with high selectivity and sensitivity. The detection limit was 47.8μM and 0.2μM for HSO3(-) and ClO(-) respectively...
October 12, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Qing Liu, Fang Li, Hao Lu, Man Li, Jing Liu, Shuangling Zhang, Qingjie Sun, Liu Xiong
Starch nanoparticles (SNPs) have attracted much research interest recently due to their biodegradability and biocompatibility. However, practical utilization of SNPs is generally restricted due to their weak colloid stability and reduced functionality. In this work, SNPs were functionally modified by sodium hypochlorite (1-5% active chlorine based on dry SNPs weight). The degree of modification, particle size, stability, and adsorption characteristics of the SNPs were systematically investigated. The results showed that as the active chlorine concentration increased, the carbonyl and carboxyl contents increased to 0...
March 1, 2018: Food Chemistry
Ebrahim Aboualizadeh, Violet V Bumah, Daniela S Masson-Meyers, Janis T Eells, Carol J Hirschmugl, Chukuka S Enwemeka
Disinfectants and biocidal products have been widely used to combat Methicillin-resistant Staphylococcus aureus (MRSA) infections in homes and healthcare environments. Although disruption of cytoplasmic membrane integrity has been documented as the main bactericidal effect of biocides, little is known about the biochemical alterations induced by these chemical agents. In this study, we used Fourier transform infrared (FT-IR) spectroscopy and chemometric tools as an alternative non-destructive technique to determine the bactericidal effects of commonly used disinfectants against MRSA USA-300...
2017: PloS One
Ricardo Abreu da Rosa, Manuela Favarin Santini, José Antônio Poli de Figueiredo, Fernanda Visioli, Jefferson Ricardo Pereira, Rodrigo Ricci Vivan, Francisco Montagner, Marcus Vinícius Reis Só
BACKGROUND: This study aimed to evaluate the antibacterial effect and the biofilm disruption promoted by antimicrobial photodynamic therapy (aPDT) associated with sodium hypochlorite (NaOCl) and chlorexidine (CHX) over monospecies and multispecies biofilms. METHODS: In monospecies model, forty-six premolars were inoculated with Enterococcus faecalis for 21days and divided into three groups: saline, CHX and NaOCl. After irrigation, aPDT was performed. Samples were collected at baseline (S1) and after irrigation (S2) and aPDT (S3)...
October 12, 2017: Photodiagnosis and Photodynamic Therapy
Manikandan Ekambaram, Robert P Anthonappa, Sharaschandra R Govindool, Cynthia Ky Yiu
OBJECTIVE: To compare bonding of dental adhesive to hypomineralized enamel (HE) after pre-treatment with either 5% sodium hypochlorite (NaOCl) solution or papain-based papacarie gel. METHODS: Normal enamel (NE) and HE obtained from hypomineralized first permanent molars were acid-etched with 32% phosphoric acid and randomly allocated into no deproteinization, deproteinization using 5% NaOCl, or deproteinization usping papacarie gel groups. Subsequently, the specimens were bonded, packed with composite resins and subjected to micro-shear bond strength (MSBS) testing and the data analysed using 2-way ANOVA and Tukey tests...
October 11, 2017: Journal of Dentistry
David Jager, Daniel Kupka, Miroslava Vaclavikova, Lucia Ivanicova, George Gallios
Degradation of commercial grade Reactive Black 5 (RB5) azo dye by chemical and electrochemical treatment was examined using a dimensionally stable anode and stainless steel cathodes as electrode materials, with NaCl as supporting electrolyte. The electrochemical treatment was compared to the chemical treatment with hypochlorite generated by electrolysis. The compounds present in the commercial grade RB5 azo dye and the products of its electrochemical degradation were separated using ion-pairing high performance liquid chromatography on reversed phase...
September 27, 2017: Chemosphere
Husam Ballout, Moritz Hertel, Jonas Doehring, Eckehard Kostka, Stefan Hartwig, Sebastian Paris, Saskia Preissner
The aim of this investigation was to evaluate the effects of two different cold atmospheric plasma (CAP) sources, photodynamic therapy and sodium hypochlorite (NaOCl), on infected root canals. Therefore, 50 standardized curved human root canals were infected with Enterococcus faecalis and assigned to five groups - negative control (NC), plasma jet (CAP I), dielectric barrier discharge (CAP II), photodynamic therapy (PDT), and NaOCl + PUI - for 30 s. Colony forming units (CFUs) were determined. NaOCl was significantly more effective at reducing CFUs than all test groups (p < ...
October 12, 2017: Journal of Biophotonics
Pascale Van Rooij, Frank Pasmans, Yanaika Coen, An Martel
The recently emerged chytrid fungus Batrachochytrium salamandrivorans (Bsal) causes European salamander declines. Proper hygiene protocols including disinfection procedures are crucial to prevent disease transmission. Here, the efficacy of chemical disinfectants in killing Bsal was evaluated. At all tested conditions, Biocidal®, Chloramine-T®, Dettol medical®, Disolol®, ethanol, F10®, Hibiscrub®, potassium permanganate, Safe4®, sodium hypochlorite, and Virkon S®, were effective at killing Bsal. Concentrations of 5% sodium chloride or lower, 0...
2017: PloS One
C Ramírez-Bommer, K Gulabivala, Y-L Ng, A Young
AIM: To characterise chemical degradation of the principal constituents of dentine after exposure to NaOCl and EDTA using Infrared Spectroscopy (ATR-FTIR). METHODOLOGY: Ground dentine particles, from extracted permanent human molars, were passed through sieves of 38 to1000 μm to provide six size ranges. Portions (250 mg) of each size range were reacted with 5mL of 2.5% NaOCl for 2-10 minutes; or 17% EDTA for 5-1440 minutes. Powders larger than 75μm were also sequentially exposed to NaOCl/EDTA/NaOCl each for 10 minutes...
October 9, 2017: International Endodontic Journal
Orianne Dumas, Aleta S Wiley, Catherine Quinot, Raphaëlle Varraso, Jan-Paul Zock, Paul K Henneberger, Frank E Speizer, Nicole Le Moual, Carlos A Camargo
Disinfectant use has been associated with adverse respiratory effects among healthcare workers. However, the specific harmful agents have not been elucidated. We examined the association between occupational exposure to disinfectants and asthma control in the Nurses' Health Study II, a large cohort of female nurses.Nurses with asthma were invited in 2014 to complete two questionnaires on their current occupation and asthma (response rate 80%). Asthma control was defined by the Asthma Control Test (ACT). Exposure to major disinfectants was evaluated by a job-task-exposure matrix (JTEM)...
October 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
Kh M El-Dakhly, A S M Aboshinaf, W M Arafa, L N Mahrous, E El-Nahass, A F Gharib, P J Holman, T M Craig
The effect of six available and commercial disinfectants on the embryonation and larval development of Toxascaris leonina eggs was studied. Dettol® and Virkon® both induced a 100% reduction in larval development (P ≤ 0.05). Dettol® resulted in deformed eggshells and a halt in embryonal development at 1 week post exposure. All Virkon®-treated eggs showed an early embryonic lysis 24 h post exposure. TH4+ and 70% ethanol both significantly (P ≤ 0.05) affected larval development, with 58.8 and 85.8% reduction, respectively...
October 4, 2017: Journal of Helminthology
E V Zambiazzi, A T Bruzi, S R Guilherme, D R Pereira, J G Lima, A M Zuffo, F O Ribeiro, A E S Mendes, S H M Godinho, M L M Carvalho
Estimating genotype x environment (GxE) parameters for quality and yield in soybean seed grown in different environments in Minas Gerais State was the goal of this study, as well as to evaluate interaction effects of GxE for soybean seeds yield and quality. Seeds were produced in three locations in Minas Gerais State (Lavras, Inconfidentes, and Patos de Minas) in 2013/14 and 2014/15 seasons. Field experiments were conducted in randomized blocks in a factorial 17 x 6 (GxE), and three replications. Seed yield and quality were evaluated for germination in substrates paper and sand, seedling emergence, speed emergency index, mechanical damage by sodium hypochlorite, electrical conductivity, speed aging, vigor and viability of seeds by tetrazolium test in laboratory using completely randomized design...
September 27, 2017: Genetics and Molecular Research: GMR
Prabal Sharma, Sandeep Garg, Nidhi Mangtani Kalra
INTRODUCTION: Denture cleansing is essential for maintaining the prosthesis and oral health, so choosing an appropriate cleanser which is not only efficient but also not adversely affects the properties of denture base resin itself with prolonged use is important. Hence, the present study was undertaken. AIM: To evaluate the effect of different chemical cleansers on surface roughness and flexural strength of heat cure denture base resin when used daily for three months...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
Matilde Ciccia, Roksana Chakrokh, Dario Molinazzi, Angela Zanni, Patrizia Farruggia, Fabrizio Sandri
AIM: The study reports a 2-year single-center experience of the practice of skin antisepsis using a 0.05% sodium hypochlorite solution before central venous catheter placement in neonates. METHODS: Eligible subjects included any hospitalized neonate who needed a central line for at least 48 hours. Infants were excluded if they had a generalized or localized skin disorder. An ad hoc Excel (Microsoft Corp, Redmond, WA) file was used to record the data from each patient...
September 26, 2017: American Journal of Infection Control
Gisselle M Chávez-Andrade, Mário Tanomaru-Filho, Elisandra M Rodrigues, Ana Lívia Gomes-Cornélio, Gisele Faria, Maria Inês B Bernardi, Juliane M Guerreiro-Tanomaru
OBJECTIVE: To evaluate the cytotoxicity, genotoxicity and antibacterial activity of poly(vinyl alcohol)-coated silver nanoparticles (AgNPs-PVA) and farnesol (FAR). DESIGN: The cytotoxicity (% of cell viability) was evaluated by MTT assay and the genotoxicity (% of DNA in the tail) was evaluated by Comet assay. Root canal disinfection with different irrigating protocols was evaluated ex vivo in human teeth contaminated with Enterococcus faecalis for 21days. Three microbiological samples were collected: initial (after contamination); post-irrigation (after irrigation); and final (after 7days)...
September 25, 2017: Archives of Oral Biology
Patricia P Wright, Bill Kahler, Laurence J Walsh
Endodontic irrigating solutions may interact chemically with one another. This is important, because even when solutions are not admixed, they will come into contact with one another during an alternating irrigation technique, forming unwanted by-products, which may be toxic or irritant. Mixing or alternating irrigants can also reduce their ability to clean and disinfect the root canal system of teeth by changing their chemical structure with subsequent loss of the active agent, or by inducing precipitate formation in the root canal system...
September 29, 2017: Materials
Sobhnamayan F, Adl A, Ghanbaran S
STATEMENT OF PROBLEM: Previous studies have shown discoloration of mineral trioxide aggregate (MTA) in contact with root canal irrigation solutions. However, there are limited data on colour stability of other calcium silicate-based materials (CSMs). OBJECTIVES: This in vitro study aimed to evaluate the colour stability of three CSMs in contact with different irrigation solutions. MATERIALS AND METHODS: Three CSMs including White MTA (wMTA) Angelus, calcium enriched mixture (CEM), and Biodentine were assessed in this study...
June 2017: J Dent Biomater
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