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Cora Roehlecke, Monika Valtink, Annika Frenzel, Doris Goetze, Lilla Knels, Henning Morawietz, Richard H W Funk
PURPOSE: Intracellular formation of advanced glycation end products (AGEs) is a crucial pathological process in retinal diseases such as age-related macular degeneration (AMD) or diabetic retinopathy (DR). Glyoxal is a physiological metabolite produced during formation of AGEs and has also been shown to derive from photodegraded bisretinoid fluorophores in aging retinal pigment epithelial (RPE) cells. METHODS: Flow cytometry was combined with either: 1) immunocytochemical staining to detect glyoxal induced formation of N(ε)-carboxymethyllysine (CML)-modifications of intracellular proteins (AGEs) and changes in the production of stress response proteins; or 2) vital staining to determine apoptosis rates (annexin V binding), formation of intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and changes in intracellular pH upon treatment of cells with glyoxal...
August 12, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
Te-Chun Hsia, Mei-Chin Yin, Mei-Chin Mong
Effects of carboxymethyllysine (CML) and pentosidine, two advanced glycation end-products (AGEs), upon invasion and migration in A549 and Calu-6 cells, two non-small cell lung cancer (NSCLC) cell lines were examined. CML or pentosidine at 1, 2, 4, 8 or 16 μmol/L were added into cells. Proliferation, invasion and migration were measured. CML or pentosidine at 4-16 μmol/L promoted invasion and migration in both cell lines, and increased the production of reactive oxygen species, tumor necrosis factor-α, interleukin-6 and transforming growth factor-β1...
2016: International Journal of Molecular Sciences
Ali Aljohi, Sabine Matou-Nasri, Nessar Ahmed
The accumulation of advanced glycation endproducts (AGEs) and oxidative stress underlie the pathogenesis of diabetic complications. In many developing countries, diabetes treatment is unaffordable, and plants such as bitter gourd (or bitter melon; Momordica charantia) are used as traditional remedies because they exhibit hypoglycaemic properties. This study compared the antiglycation and antioxidant properties of aqueous extracts of M. charantia pulp (MCP), flesh (MCF) and charantin in vitro. Lysozyme was mixed with methylglyoxal and 0-15 mg/ml of M...
2016: PloS One
Ma Etzabel Villegas-Rodríguez, Jaime Uribarri, Sergio E Solorio-Meza, Martha E Fajardo-Araujo, Weijing Cai, Sofía Torres-Graciano, Rubén Rangel-Salazar, Kazimierz Wrobel, Ma Eugenia Garay-Sevilla
AIM: The purpose of the study was the simultaneous measurement of all the different components of the AGE-RAGE axis as well as several non-invasive markers of cardiovascular disease (CVD) in a cohort of newly diagnosed diabetic patients. MATERIALS AND METHODS: In 80 newly diagnosed diabetic patients we measured serum carboxymethyllysine (CML), soluble RAGE (sRAGE) and peripheral mononuclear (PMNC) RAGE and AGER1 mRNA together with ICAM-1, VCAM-1, and malondialdehyde (MDA)...
2016: PloS One
Frédéric J Tessier, Céline Niquet-Léridon, Philippe Jacolot, Céline Jouquand, Michaël Genin, Ann-Marie Schmidt, Nicolas Grossin, Eric Boulanger
SCOPE: N(ɛ) -Carboxymethyl-lysine (CML) is a prominent advanced glycation end-product which is not only found in vivo but also in food. It is known that a percentage of the dietary CML (dCML) is absorbed into the circulation and only partly excreted in the urine. Several studies have tried to measure how much dCML remains in tissues. However obstacles to interpreting the data have been found. METHODS AND RESULTS: A new protocol which discriminates dCML from native CML (nCML) has been developed...
July 9, 2016: Molecular Nutrition & Food Research
Janusz Strzelczyk, Magdalena Szumska, Aleksandra Damasiewicz-Bodzek, Anna Krywult, Michał Długaszek, Justyna Czubilińska, Kaja Gawlik, Konrad Synowiec, Krystyna Tyrpień-Golder, Karolina Poczkaj, Beata Kos-Kudła
INTRODUCTION: The glycation process is a non-enzymatic modification of proteins occurring due to the reactions of reductive carbohydrates. The glycated residues lose their biological functions, and their removal process is ineffective. They accumulate, and as a result they cause an immunological response. The aim of this study was a determination of the concentrations of advanced glycation end-products and antibodies against carboxymethyl lysine (anti-CML) and carboxyethyl lysine (anti-CEL) in the sera of Graves' orbitopathy patients...
2016: Endokrynologia Polska
Huilin Liu, Dan Wu, Kaiwen Zhou, Jing Wang, Baoguo Sun
A novel molecularly imprinted polymer (MIP) based on hydrophobic CdSe/ZnS quantum dots (HBQDs) was synthesized via an approach that combines MIPs for the selectivity and sensitivity with semiconductor HBQDs as optosensing material (HBQDs@MIP). Here, using a one-pot water-in-oil reverse microemulsion polymerization, the HBQDs@MIP was used as a molecular recognition element to construct an N(ε)-carboxymethyllysine (CML) optosensor. The HBQDs were coated with silica to endow the nanoparticles with high dispersibility and broad compatibility with hydrophilic analytes...
November 15, 2016: Food Chemistry
Huilin Liu, Xiaomo Chen, Lin Mu, Jing Wang, Baoguo Sun
Hydrophobic CdSe/ZnS quantum dots (QDs) coated with a molecularly imprinted polymer (MIP) sensitized with graphene (Gra-QDs@MIP) were prepared through a one-pot reverse microemulsion polymerization at room temperature. Gra-QDs@MIP was used as a molecular recognition element to construct a N(ε)-carboxymethyllysine (CML) optosensor. Graphene was used as a polymerization platform to increase the stability and kinetic binding properties of the system. Reverse microemulsion polymerization can anchor silica spheres on the surface of the QDs...
June 15, 2016: Journal of Agricultural and Food Chemistry
Mona S Calvo, Anita Mehrotra, Robert B Beelman, Girish Nadkarni, Lingzhi Wang, Weijing Cai, Boon Cher Goh, Michael D Kalaras, Jaime Uribarri
Adults with metabolic syndrome from different race/ethnicities are often predisposed to developing type 2 diabetes (T2D); however, growing evidence suggests that healthy diets and lifestyle choices can significantly slow or prevent progression to T2D. This poorly understood relationship to healthy dietary patterns and prevention of T2D motivated us to conduct a retrospective analysis to determine the potential impact of a minor dietary lifestyle change (daily mushroom consumption) on known T2D risk factors in racially diverse adults with confirmed features of the metabolic syndrome...
September 2016: Plant Foods for Human Nutrition
Daiva Stanislovaitienė, Dalia Žaliūnienė, Rasa Steponavičiūtė, Reda Žemaitienė, Olivija Gustienė, Remigijus Žaliūnas
BACKGROUND AND OBJECTIVE: An association between coronary artery disease (CAD) and age-related macular degeneration (ARMD) has long been postulated, but exact mechanisms remain unclear. The global prevalence of CAD and ARMD increases and early biomarkers for early diagnosis of these diseases are necessary. The aim of this study was to investigate the plasma level of oxidative stress biomarker CML in patients with and without angiographic findings of atherosclerosis in the coronary arteries (CADath+ and CADath-, respectively) and to assess if there was an association of CAD with ARMD...
2016: Medicina
Ann-Katrin Holik, Barbara Lieder, Nicole Kretschy, Mark M Somoza, Sandra Held, Veronika Somoza
Advanced glycation endproducts, formed in vivo, but also by the Maillard reaction upon thermal treatment of foods, have been associated with the progression of pathological conditions such as diabetes mellitus. In addition to the accumulation with age, exogenous AGEs are introduced into the circulation from dietary sources. In this study, we investigated the effects of addition of free N(ϵ) -carboxymethyllysine (CML), a well-characterized product of the Maillard reaction, on adipogenesis in 3T3-L1 preadipocytes...
October 2016: Journal of Cellular Biochemistry
T G Hulshof, P Bikker, A F B van der Poel, W H Hendriks
An experiment was conducted to determine protein quality in processed protein sources using the content of AA, -methylisourea (OMIU)-reactive Lys, Maillard reaction products (MRP), and cross-link products; the standardized ileal digestibility (SID) of CP and AA; and growth performance in growing pigs as criteria. Differences in protein quality were created by secondary toasting (at 95°C for 30 min) of soybean meal (SBM) and rapeseed meal (RSM) in the presence of lignosulfonate resulting in processed SBM (pSBM) and processed RSM (pRSM)...
March 2016: Journal of Animal Science
Saurabh Awasthi, Kamatchi Sankaranarayanan, N T Saraswathi
Glycation induced amyloid fibrillation is fundamental to the development of many neurodegenerative and cardiovascular complications. Excessive non-enzymatic glycation in conditions such as hyperglycaemia results in the increased accumulation of advanced glycation end products (AGEs). AGEs are highly reactive pro-oxidants, which can lead to the activation of inflammatory pathways and development of oxidative stress. Recently, the effect of non-enzymatic glycation on protein structure has been the major research area, but the role of specific AGEs in such structural alteration and induction of fibrillation remains undefined...
June 15, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Ulrike Moeckel, Anja Duerasch, Alexander Weiz, Michael Ruck, Thomas Henle
After suspensions of micellar casein or nonmicellar sodium caseinate had been heated, respectively, in the presence and absence of glucose for 0-4 h at 100 °C, glycation compounds were quantitated. The formation of Amadori products as indicators for the "early" Maillard reaction were in the same range for both micellar and nonmicellar caseins, indicating that reactive amino acid side chains within the micelles are accessible for glucose in a comparable way as in nonmicellar casein. Significant differences, however, were observed concerning the formation of the advanced glycation end products (AGEs), namely, N(ε)-carboxymethyllysine (CML), pyrraline, pentosidine, and glyoxal-lysine dimer (GOLD)...
April 13, 2016: Journal of Agricultural and Food Chemistry
Cynthia Helou, Pascale Gadonna-Widehem, Nathalie Robert, Gérard Branlard, Jacques Thebault, Sarah Librere, Sylvain Jacquot, Julie Mardon, Agnès Piquet-Pissaloux, Sophie Chapron, Antoine Chatillon, Céline Niquet-Léridon, Frédéric J Tessier
The aim of this study was to develop a white bread with improved nutrient contents and reduced levels of potentially harmful Maillard reaction products such as N(ε)-carboxymethyllysine (CML) and 5-hydroxymethylfurfural (HMF). Assays were carried out through a full factorial experimental design allowing the simultaneous analysis of four factors at two levels: (1) wheat flour extraction rates (ash content: 0.60%-0.72%), (2) leavening agents (bakers' yeast - bakers' yeast and sourdough), (3) prebaking and (4) baking conditions (different sets of time and temperature)...
June 15, 2016: Food & Function
Stéphane Jaisson, Pierre-François Souchon, Aurore Desmons, Anne-Sophie Salmon, Brigitte Delemer, Philippe Gillery
OBJECTIVES: To quantify serum advanced glycation end-products (AGEs) at the onset of type 1 diabetes mellitus and to determine their potential usefulness as retrospective indicators of glycemic balance. STUDY DESIGN: Carboxymethyllysine (CML) and pentosidine concentrations were determined by liquid chromatography-tandem mass spectrometry in 3 groups of children with type 1 diabetes mellitus: group (Gr) 1, subjects included at disease onset (n = 36); Gr2, subjects with diabetes of 5 years duration (n = 48); Gr3, subjects with diabetes of 10 years duration and in control subjects (n = 33)...
May 2016: Journal of Pediatrics
Anand Prasad, James R Lane, Sotirios Tsimikas, Ehtisham Mahmud, Srikrishna Khandrika, Peter Bekker, Manjusha Ilapakurti, Dan Nguyen, Amir Ravandi, Travis Israel
Evidence implicates a role of advanced glycation end products (AGEs) in the development of atherosclerosis. The present study examined the relationship between plasma levels of AGEs and the clinical and angiographic characteristics of patients with symptomatic peripheral arterial disease (PAD). A total of 40 consecutive patients with symptomatic lower extremity PAD undergoing invasive evaluation were enrolled. Clinical history, angiographic data, and plasma levels of total AGE (tAGE), N'-carboxymethyllysine (CML), and high-sensitivity C-reactive protein were obtained...
March 2016: International Journal of Angiology: Official Publication of the International College of Angiology, Inc
La Salete Martins, José C Oliveira, José Ramón Vizcaíno, Isabel Fonseca, Carlos Gouveia, Donzília Silva, António C Henriques, Irene L Noronha, Anabela Rodrigues
Diabetes mellitus leads to increased Advanced Glycation End Products (AGE) production, which has been associated with secondary diabetic complications. Type 1 diabetic patients undergoing pancreas-kidney transplantation (SPKT) can restore normoglycemia and renal function, eventually decreasing AGE accumulation. We aimed to prospectively study AGE evolution after SPKT. Circulating AGE were assessed in 20 patients, at time 0 (T0), 3 months (T3), 6 months (T6), and 12 months (T12) after successful SPKT. Global AGE and carboxymethyllysine (CML) were analyzed, as well as advanced oxidation protein products (AOPP)...
2016: Oxidative Medicine and Cellular Longevity
Xiaohua Sun, Juming Tang, Jing Wang, Barbara A Rasco, Keqiang Lai, Yiqun Huang
The effect of commercial sterilization treatments on the levels of advanced glycation endproducts (AGEs) in meats was investigated. The amounts of both free and protein-bound N(ε)-carboxymethyllysine (CML) and N(ε)-carboxyethyllysine (CEL) in beef (rump, ribeye, short plate), pork (hind leg, tenderloin, belly), and chicken (chicken breasts, drumsticks) were determined using an HPLC-MS/MS method. Beef and pork had a small proportion (raw <15%; sterilized <8%) of free AGEs compared to the total AGEs, but raw chicken breasts had very high levels of free CEL (7...
June 2016: Meat Science
C van Rooijen, G Bosch, C I Butré, A F B van der Poel, P A Wierenga, L Alexander, W H Hendriks
During processing of foods, the Maillard reaction occurs, resulting in the formation of advanced Maillard reaction products (MRP). Varying amounts of MRP have been found in commercially processed pet foods. Dietary MRP can be absorbed and contribute to the endogenous pool of MRP and possibly the etiology of age-related diseases. The aim of the present study was to determine urinary excretion of dietary MRP in cats fed commercial moist and dry foods. A pilot study with 10 cats, conducted to determine the adaptation time required for stable urinary excretion of MRP when changing to a diet with contrasting MRP content, showed an adaptation time of 1 d for all components...
January 2016: Journal of Animal Science
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