Andreas Lindqvist, Mia Abels, Liliya Shcherbina, Mtakai Ngara, Dmytro Kryvokhyzha, Sabrina Chriett, Matteo Riva, Abul Fajul, Mohammad Barghouth, Cheng Luan, Lena Eliasson, Olav Larsen, Mette M Rosenkilde, Enming Zhang, Erik Renström, Nils Wierup
Cocaine and amphetamine-regulated transcript (CART) is expressed in pancreatic islet cells and neuronal elements. We have previously established insulinotropic actions of CART in human and rodent islets. The receptor for CART in the pancreatic beta cells is unidentified. We used RNA sequencing of Cartpt knockdown (KD) INS-1 832/13 cells and identified GPR162 as the most Cartpt -regulated receptor. We therefore tested if GPR162 mediates the effects of CART in beta cells. Binding of CART to GPR162 was established using proximity ligation assay, radioactive binding, and co-immunoprecipitation, and KD of Gpr162 mRNA caused reduced binding...
December 15, 2023: IScience