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https://www.readbyqxmd.com/read/29787378/larval-hemolymph-of-rhinoceros-beetle-allomyrina-dichotoma-enhances-insulin-secretion-through-atf3-gene-expression-in-ins-1-pancreatic-%C3%AE-cells
#1
Seung-Whan Kim, Hyun-Woo Suh, Bo-Kyung Yoo, Kisang Kwon, Kweon Yu, Ji-Young Choi, O-Yu Kwon
In this study, we show that INS-1 pancreatic β-cells treated for 2 h with hemolymph of larvae of rhinoceros beetle, Allomyrina dichotoma, secreted about twice as much insulin compared to control cells without such treatment. Activating transcription factor 3 (ATF3) was the highest upregulated gene in DNA chip analysis. The A. dichotoma hemolymph dose-dependently induced increased expression levels of genes encoding ATF3 and insulin. Conversely, treatment with ATF3 siRNA inhibited expression levels of both genes and curbed insulin secretion...
May 22, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/29772703/alpha-mangostin-improves-insulin-secretion-and-protects-ins-1-cells-from-streptozotocin-induced-damage
#2
Dahae Lee, Young-Mi Kim, Kiwon Jung, Young-Won Chin, Ki Sung Kang
Alpha (α)-mangostin, a yellow crystalline powder with a xanthone core structure, is isolated from mangosteen ( Garcinia mangostana ), which is a tropical fruit of great nutritional value. The aim of the present study was to investigate the anti-diabetic effects of α-mangostin and to elucidate the molecular mechanisms underlying its effect on pancreatic beta (β)-cell dysfunction. To assess the effects of α-mangostin on insulin production, rat pancreatic INS-1 cells were treated with non-toxic doses of α-mangostin (1⁻10 μM) and its impact on insulin signaling was examined by Western blotting...
May 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29755519/simulated-cholinergic-reinnervation-of-%C3%AE-ins-1-cells-antidiabetic-utility-of-heterotypic-pseudoislets-containing-%C3%AE-cell-and-cholinergic-cell
#3
Ao Jiao, Feng Li, Chengshuo Zhang, Wu Lv, Baomin Chen, Jialin Zhang
Cholinergic neurons can functionally support pancreatic islets in controlling blood sugar levels. However, in islet transplantation, the level of cholinergic reinnervation is significantly lower compared to orthotopic pancreatic islets. This abnormal reinnervation affects the survival and function of islet grafts. In this study, the cholinergic reinnervation of beta cells was simulated by 2D and 3D coculture of INS-1 and NG108-15 cells. In 2D culture conditions, 20 mM glucose induced a 1.24-fold increase ( p < 0...
2018: International Journal of Endocrinology
https://www.readbyqxmd.com/read/29754287/protective-role-of-the-elovl2-docosahexaenoic-acid-axis-in-glucolipotoxicity-induced-apoptosis-in-rodent-beta-cells-and-human-islets
#4
Lara Bellini, Mélanie Campana, Claude Rouch, Marta Chacinska, Marco Bugliani, Kelly Meneyrol, Isabelle Hainault, Véronique Lenoir, Jessica Denom, Julien Véret, Nadim Kassis, Bernard Thorens, Mark Ibberson, Piero Marchetti, Agnieszka Blachnio-Zabielska, Céline Cruciani-Guglielmacci, Carina Prip-Buus, Christophe Magnan, Hervé Le Stunff
AIMS/HYPOTHESIS: Dietary n-3 polyunsaturated fatty acids, especially docosahexaenoic acid (DHA), are known to influence glucose homeostasis. We recently showed that Elovl2 expression in beta cells, which regulates synthesis of endogenous DHA, was associated with glucose tolerance and played a key role in insulin secretion. The present study aimed to examine the role of the very long chain fatty acid elongase 2 (ELOVL2)/DHA axis on the adverse effects of palmitate with high glucose, a condition defined as glucolipotoxicity, on beta cells...
May 12, 2018: Diabetologia
https://www.readbyqxmd.com/read/29753073/glycine-transporter-1-and-glycine-receptor-mediate-the-antioxidant-effect-of-glycine-in-diabetic-rat-islets-and-ins-1-cells
#5
Lei Chen, Junqing Zhang, Changhong Li, Ziwei Wang, Jingjing Li, Dan Zhao, Suxia Wang, Hong Zhang, Youyuan Huang, Xiaohui Guo
Oxidative stress is the main inducer of β-cell damage, which underlies the pathogenesis of diabetes. Evidence suggests that glycine, a recognized antioxidant, may improve β-cell function; however, its mechanism in protecting diabetic β-cells against oxidative stress has not been directly investigated. Using a streptozotocin-induced diabetic rat model and INS-1 pancreatic β-cells, we evaluated whether glycine can attenuate diabetic β-cell damage induced by oxidative stress. In diabetic rats, glycine stimulated insulin secretion; enhanced plasma glutathione (GSH), catalase and superoxide dismutase levels; reduced plasma 8-hydroxy-2 deoxyguanosine and islet p22phox levels; and improved islet β-cell mitochondrial degeneration and insulin granule degranulation...
May 9, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29750032/protection-of-silver-nanoparticles-using-eysenhardtia-polystachya-in-peroxide-induced-pancreatic-%C3%AE-cell-damage-and-their-antidiabetic-properties-in-zebrafish
#6
Abraham Heriberto Garcia Campoy, Rosa Martha Perez Gutierrez, Gabriela Manriquez-Alvirde, Alethia Muñiz Ramirez
Background: The aim was to explore the efficacy of extract of Eysenhardtia polystachya -loaded silver nanoparticles (EP/AgNPs) on pancreatic β cells, INS-1 cells, and zebrafish as a valuable model for the study of diabetes mellitus. Materials and methods: EP/AgNPs were synthesized using methanol/water bark extract of E. polystachya and characterized using various physicochemical techniques. Results: Immersion of adult zebrafish in 111 mM glucose solution resulted in a sustained hyperglycemic, hyperlipidemic state, and serum insulin levels decreased...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29725251/glucagon-like-peptide-1-receptor-agonist-and-glucagon-increase-glucose-stimulated-insulin-secretion-in-beta-cells-via-distinct-adenylyl-cyclases
#7
Young-Sun Lee, Hee-Sook Jun
Diabetes mellitus is a chronic disease in which the pancreas no longer produces enough insulin. Pancreatic alpha cell mass increases in response to insufficient insulin secretion. However, the reason for this increase is not clear. It is possible that the increased alpha-cells may stimulate compensatory insulin release in response to the insufficient insulin such as insulin resistance. In this study, we investigated whether glucagon and glucagon-like peptide-1 (GLP-1), hormones produced by alpha cells, contribute to insulin secretion in INS-1 cells, a beta cell line...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29691580/-adenovirus-mediated-overexpression-of-thioredoxin-interaction-protein-inhibits-ins-1-islet-%C3%AE-cell-proliferation
#8
Zhu-Jie Cao, Yan-Jin Feng, Dan Li, Jin Wang, Hai-Yan Huo, Xu-Mei Zhang, Xiang-Ying Jiao
Diabetes can cause a significant increase in the expression of thioredoxin (Trx)-interacting protein (TXNIP), which binds to Trx and inhibits its activity. The present study was aimed to investigate the effect of TXNIP on proliferation of rat INS-1 islet β cells and the underlying mechanism. TXNIP overexpressing adenovirus vectors (Ad-TXNIP-GFP and Ad-TXNIPc247s-GFP) were constructed and used to infect INS-1 cells. Ad-TXNIPc247s-GFP vector carries a mutant C247S TXNIP gene, and its expression product (TXNIPc247s) cannot attach and inhibit Trx activity...
April 25, 2018: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/29691579/-angiotensin-ii-promotes-the-expression-of-txnip-through-angiotensin-ii-type-1-receptor-in-islet-%C3%AE-cells
#9
Yan-Jin Feng, Jin Wang, Zhu-Jie Cao, Dan Li, Hai-Yan Huo, Xu-Mei Zhang, Xiang-Ying Jiao
This study investigated the effect of angiotensin II (Ang II) on apoptosis and thioredoxin-interacting protein (TXNIP) expression in INS-1 islet cells and the underlying mechanism. INS-1 cells cultured in vitro were treated with different concentration of Ang II for different time, and the viability was measured using cell counting kit-8 (CCK-8). After treatment with 1 × 10-6 mol/L Ang II for 24 h, flow cytometry and Western blot were used to measure the cell apoptosis, and Western blot was used to analyze the protein expression of TXNIP, carbohydrate response element-binding protein (ChREBP) and angiotensin II type 1 receptor (AT1R)...
April 25, 2018: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/29678908/the-lim-homeodomain-protein-isl1-mediates-the-function-of-tcf7l2-in-pancreatic-beta-cells
#10
Weijuan Shao, Vivian Szeto, Zhuolun Song, Lili Tian, Zhong-Ping Feng, Cristina M Nostro, Tianru Jin
Pancreatic β-cell Tcf7l2 deletion or its functional knockdown suggested the essential role of this Wnt pathway effector in controlling insulin secretion, glucose homeostasis, and β-cell gene expression. As the LIM homeodomain protein Isl1 is a suggested Wnt pathway downstream target, we hypothesize that it mediates metabolic functions of Tcf7l2. We aimed to determine the role of Isl1 in mediating the function of Tcf7l2 and the incretin hormone GLP-1 in pancreatic β-cells. Effect of dominant negative TCF7L2 (TCF7L2DN) mediated Wnt pathway functional knockdown on Isl1 expression was determined in βTCFDN mouse islets and in the rat insulinoma cell line INS-1 832/13...
April 20, 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29620270/pioglitazone-microrna%C3%A2-141-foxa2-a-novel-axis-in-pancreatic-%C3%AE-%C3%A2-cells-proliferation-and-insulin-secretion
#11
Xin Yu, Liyong Zhong
MicroRNAs (miRs) are considered to be effective, post‑transcriptional regulators in the pathophysiology of type 2 diabetes (T2D) and promising treatment targets. However, the function of miR‑141 remains to be elucidated. In the present study, upregulation of miR‑141 was demonstrated in diabetic mice and elderly diabetic patients. Using reverse transcriptase‑quantitative polymerase chain reaction, luciferase reporter assays and western blotting, forkhead box A2 (FOXA2) was identified as a direct target gene of miR‑141...
March 28, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29587416/knocking-down-insulin-receptor-in-pancreatic-beta-cell-lines-with-lentiviral-small-hairpin-rna-reduces-glucose-stimulated-insulin-secretion-via-decreasing-the-gene-expression-of-insulin-glut2-and-pdx1
#12
Jie Wang, Wenyi Gu, Chen Chen
Type 2 diabetes (T2D) is a metabolic disorder characterized by beta cell dysfunction and insulin resistance in fat, muscle and liver cells. Recent studies have shown that the development of insulin resistance in pancreatic beta cell lines may contribute to beta cell dysfunction in T2D. However, there still is a lack of detailed investigations regarding the mechanisms by which insulin deficiency may contribute in diabetes. In this study, we firstly established a stable insulin receptor knockdown cell line in pancreatic beta cells INS-1 (InsRβKD cells) using anti InsRβ small hairpin RNA (InsRβ-shRNA) encoded by lentiviral vectors...
March 26, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29571019/involvement-of-estrogen-receptors-in-silibinin-protection-of-pancreatic-%C3%AE-cells-from-tnf%C3%AE-or-il-1%C3%AE-induced-cytotoxicity
#13
Jing Yang, Yue Sun, Fanxing Xu, Weiwei Liu, Toshihiko Hayashi, Satoshi Onodera, Shin-Ichi Tashiro, Takashi Ikejima
Silibinin is a polyphenolic flavonoid that exhibits anticarcinogenic, anti-inflammatory and cytoprotective effects. The effect of silibinin on pancreatic islet β-cell is yet largely unknown in spite of well documented-hepatoprotective effects. Protecting the functional insulin-producing β-cells in the pancreas is a major therapeutic challenge in the patients with type 1 (T1DM) or type 2 diabetes mellitus (T2DM). This study reports the effect of silibinin on the rat pancreatic β-cell line, INS-1, damaged with pro-inflammatory cytokine, TNFα or IL-1β...
March 20, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29565305/mitochondrial-morphology-and-function-of-the-pancreatic-%C3%AE-cells-ins-1-model-upon-chronic-exposure-to-sub-lethal-cadmium-doses
#14
Adeline Jacquet, Cécile Cottet-Rousselle, Josiane Arnaud, Kevin Julien Saint Amand, Raoua Ben Messaoud, Marine Lénon, Christine Demeilliers, Jean-Marc Moulis
The impact of chronic cadmium exposure and slow accumulation on the occurrence and development of diabetes is controversial for human populations. Islets of Langerhans play a prominent role in the etiology of the disease, including by their ability to secrete insulin. Conversion of glucose increase into insulin secretion involves mitochondria. A rat model of pancreatic β-cells was exposed to largely sub-lethal levels of cadmium cations applied for the longest possible time. Cadmium entered cells at concentrations far below those inducing cell death and accumulated by factors reaching several hundred folds the basal level...
March 22, 2018: Toxics
https://www.readbyqxmd.com/read/29553151/evaluating-the-effects-of-iadhfl-on-inhibiting-dpp-iv-activity-and-expression-in-caco-2-cells-and-contributing-to-the-amount-of-insulin-released-from-ins-1-cells-in-vitro
#15
Chi Zhang, Huaigao Liu, Shangwu Chen, Yongkang Luo
Dipeptidyl peptidase-IV (DPP-IV) is a serine exo-peptidase that can inactivate incretins by removing N-terminal dipeptides. Currently, inhibiting the DPP-IV activity is a common treatment for type 2 diabetes (T2D). The goal of this study is to investigate whether IADHFL, a novel DPP-IV inhibitory peptide identified from bighead carp (Hypophthalmichthys nobilis), has the potential to modulate T2D. IADHFL remained stable after simulated gastrointestinal digestion and significantly decreased the activity and expression of both soluble and membrane-bound DPP-IV after 24 h and 48 h of treatment...
April 25, 2018: Food & Function
https://www.readbyqxmd.com/read/29541192/pseudo-hemorrhagic-region-formation-in-pancreatic-neuroendocrine-tumors-is-a-result-of-blood-vessel-dilation-followed-by-endothelial-cell-detachment
#16
Zai Wang, Liang Peng, Yu-Li Song, Shiqing Xu, Zhan Hua, Ni Fang, Min Zhai, Honglin Liu, Qing Fang, Tingting Deng, Wenjian Zhang, Yuan-Jia Chen, Jinning Lou
Aberrant blood vessel formation and hemorrhage may contribute to tumor progression and are potential targets in the treatment of several types of cancer. Pancreatic neuroendocrine tumors (PNETs) are highly vascularized, particularly when they are well-differentiated. However, the process of vascularization and endothelial cell detachment in PNETs is poorly understood. In the present study, 132 PNET clinical samples were examined and a special type of hemorrhagic region was observed in ~30% of the samples regardless of tumor subtype...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29525672/isoferulic-acid-attenuates-methylglyoxal-induced-apoptosis-in-ins-1-rat-pancreatic-%C3%AE-cell-through-mitochondrial-survival-pathways-and-increasing-glyoxalase-1-activity
#17
Aramsri Meeprom, Catherine B Chan, Weerachat Sompong, Sirichai Adisakwattana
Methylglyoxal (MG) is a reactive precursor to advanced glycation end-products (AGEs), which exert deleterious effects on cells and tissues. MG also causes pancreatic β-cell dysfunction and apoptosis. Isoferulic acid (IFA), a naturally occurring cinnamic acid derivative, is considered to be an antiglycating agent. However, the effect of IFA on MG-induced pancreatic β-cell dysfunction remains unknown. The objective of this study was to determine the protective effect of IFA against MG-induced mitochrondrial dysfunction and apoptosis in INS-1 pancreatic β-cells...
May 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29523142/lipid-accelerating-the-fibril-of-islet-amyloid-polypeptide-aggravated-the-pancreatic-islet-injury-in-vitro-and-in-vivo
#18
Xiao-Dan Mo, Li-Ping Gao, Qing-Jun Wang, Jie Yin, Yu-Hong Jing
BACKGROUND: The fibrillation of islet amyloid polypeptide (IAPP) triggered the amyloid deposition, then enhanced the loss of the pancreatic islet mass. However, it is not clear what factor is the determinant in development of the fibril formation. The aim of this study is to investigate the effects of lipid on IAPP fibril and its injury on pancreatic islet. METHODS: The fibril form of human IAPP (hIAPP) was tested using thioflavin-T fluorescence assay and transmission electron microscope technology after incubated with palmitate for 5 h at 25 °C...
March 9, 2018: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29514930/inositol-polyphosphate-multikinase-regulation-of-trypanosoma-brucei-life-stage-development
#19
Igor Cestari, Atashi Anupama, Kenneth Stuart
Many cellular processes change during the Trypanosoma brucei life cycle as this parasite alternates between the mammalian host and tsetse fly vector. We show that the inositol phosphate pathway helps regulate these developmental changes. Knockdown of inositol polyphosphate multikinase (IPMK), which phosphorylates Ins(1,4,5)P3 and Ins(1,3,4,5)P4, resulted in changes in bloodstream forms that are characteristic of insect stage procyclic forms. These changes include expression of the procyclic surface coat, up-regulation of RNA-binding proteins that we show to regulate stage-specific transcripts, and activation of oxidative phosphorylation with increased ATP production in bloodstream forms...
May 1, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29514130/effects-of-polysaccharide-from-portulaca-oleracea-l-on-voltage-gated-na-channel-of-ins-1-cells
#20
Qingjuan Hu, Yuping Li, Chao Liu, Li-Ping Huang, Lvming Zeng, Songhua Wang, Hao Song, Hailong Peng, Jianhua Huang, Chong Chen, Li-Hua Yao
Our previous work showed that polysaccharide isolated from Portulaca oleracea L. (POP) has an insulinotropic effect. The voltage-gated Na+ channel (VGSC) in the excitement phase plays an important role. This work aims to study the effect of POP on the voltage-gated Na+ channel current (INa ) and its channel dynamic characteristics in insulin-secreting β-cell line (INS-1) cells of rat. Our results revealed that POP can inhibit the amplitude of INa and improve cell survival in a concentration-dependent manner...
May 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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